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Choroid

The choroid is a thin, vascularized layer of tissue forming part of the , the middle tunic of the eye, situated between the (the white outer layer) and the at the back of the eyeball. It spans the posterior two-thirds of the eye, extending from the to the ora serrata, and is characterized by its dense network of blood vessels embedded in rich in melanocytes. With a thickness varying from approximately 100 to 370 micrometers depending on age, location, and measurement method (thickest subfoveally), the choroid plays a critical role in ocular . Structurally, the choroid consists of several distinct layers: the outermost suprachoroid, containing and melanocytes; Haller's layer with large vessels; Sattler's layer with medium-sized vessels; and the innermost choriocapillaris, a bed adjacent to , which separates it from the . This multilayered organization supports its high blood flow—estimated at rates up to ten times that of the per unit weight—facilitating and oxygen delivery to the avascular outer and optic nerve head. The melanin's abundance in the choroid absorbs stray light, preventing internal reflections that could degrade and protecting photoreceptors from . Beyond its vascular and optical functions, the choroid contributes to ocular through barrier properties, , and modulation of eye growth. The choriocapillaris acts as a selective barrier, regulating fluid and protein exchange between the bloodstream and retinal interstitium via fenestrated . It also secretes growth factors such as transforming growth factor-beta (TGF-β) and , influencing scleral remodeling and emmetropization—the process aligning refractive development with visual demands—and diurnal variations in choroidal thickness (approximately 20-35 micrometers) help maintain retinal apposition. Clinically, choroidal dysfunction underlies conditions like choroiditis (), age-related (via impaired permeability), and progression, often diagnosed through imaging modalities such as .

Anatomy

Macroscopic structure

The choroid constitutes the middle vascular layer of the , positioned between the externally and the internally within the posterior segment of the eye. It forms a thin, dark brown membrane due to its high content, which aids in light absorption. This layer covers the posterior two-thirds of the eyeball, extending from the posteriorly to the ora serrata anteriorly. The choroid exhibits a roughly cup-shaped configuration that conforms to the spherical of the eyeball. Its thickness varies regionally, ranging from approximately 50 to 350 μm in adults, thickest in the subfoveal region, with greater dimensions observed temporally and in posterior areas compared to nasal and anterior regions. At birth, the average thickness measures approximately 0.2 mm, progressively thinning to about 0.08 mm by age 90. The choroid adheres externally to the via forming the suprachoroidal space, a potential cavity that allows limited mobility between layers. Internally, it connects to the through , a thin . This structure is perforated by the at its posterior termination and by ciliary vessels, facilitating passage to adjacent ocular components. Notable macroscopic features include the inner adjacency of the choriocapillaris, a directly apposed to , and the presence of perivascular spaces surrounding larger vessels, which exhibit regional variations in density.

Microscopic structure

The choroid exhibits a distinct layered at the microscopic level, typically divided into four principal layers from outer to inner: the suprachoroid, the vascular layer, the choriocapillaris, and the interface with . The outermost suprachoroid, also known as the lamina fusca, consists of a thin layer of rich in melanocytes and fibers, which anchors the choroid to the and contributes to its pigmentation. Beneath this lies the vascular layer, subdivided into medium-sized vessels (Sattler's layer) and larger vessels (Haller's layer), forming a network that supports the choroid's high vascular density. The innermost choriocapillaris is a lobular directly adjacent to , facilitating nutrient exchange with the , while itself serves as a thin, pentalaminar extracellular barrier at the choroid-retinal pigment epithelium interface. At the cellular level, the choroid is composed primarily of melanocytes, which provide dense pigmentation to absorb and prevent internal reflections, particularly prominent in humans. Endothelial cells line the extensive vascular , with fibroblasts producing the supportive and immune cells such as macrophages and dendritic cells residing within the for local immune . and cells also contribute to vascular stability in the medium and large vessel layers. The of the choroid is characterized by that accommodates its vascular elements, containing primarily types I and III in the stroma, type IV in basement membranes, and fibers that impart elasticity to the vessel walls. This matrix supports the choroid's flexibility and permeability while maintaining structural integrity. Notable microscopic features include the fenestrated of the choriocapillaris, which features numerous pores allowing high permeability for components to reach the outer . Additionally, the choroid lacks conventional lymphatic vessels, relying instead on venous for clearance.

Vascular supply

The choroid receives its arterial supply primarily from the short posterior ciliary arteries, which arise as branches of the and typically number between 10 and 20 per eye. These arteries penetrate the in a radial pattern surrounding the head, branching into larger-caliber vessels that form the outer and middle vascular layers of the choroid, supplying the majority of its posterior and central extent. Two , also branching from the , provide supply to the anterior periphery of the choroid, extending forward to form an anastomotic ring near the ora serrata and contributing to the vascular network in this region. Venous drainage from the choroid occurs through four to five vortex veins, which collect blood from postcapillary venules and pierce the at sites posterior to the equator before emptying into the superior and inferior ophthalmic veins. The choroid contains no true lymphatic vessels, though perivascular spaces surrounding the choroidal vasculature facilitate movement and exchange. The short posterior ciliary arteries account for the bulk of choroidal , penetrating the at multiple discrete sites to distribute across the ; within the inner choriocapillaris layer, this results in a high vascular organized into numerous lobules. Choroidal blood flow is autoregulated mainly via a myogenic response in the cells of the medium and large vessels, helping maintain stable despite fluctuations in perfusion pressure. The choroidal circulation features high flow rates with low oxygen extraction, typically around 3-5% from the passing through the .

Function

Nutritional support

The choroid plays a crucial role in oxygen delivery to the outer and (RPE) primarily through from the , a fenestrated layer adjacent to . This vascular network supplies the majority of oxygen required by the photoreceptors and outer retinal layers, where metabolic demand is high due to phototransduction processes. Studies indicate that under normal conditions, the choroid accounts for approximately 50-60% of total retinal oxygen consumption, with the potential to supply up to 97% of the oxygen needs between the choriocapillaris and deep retinal capillaries during hyperoxic states. The exceptionally high blood flow rate in the choroid, estimated at around 800 ml per 100 g of per minute—one of the highest in the —facilitates this efficient oxygen transfer by maintaining a steep gradient across the RPE. Nutrient transport from the choroid to the outer occurs via passive diffusion and active mechanisms across , enabling the delivery of essential metabolites such as glucose, , and fatty acids. The fenestrated of the choriocapillaris allows high permeability to small molecules like glucose and for subsequent uptake by the RPE and photoreceptors. Glucose, the primary energy substrate for the , is shuttled through RPE transporters (e.g., ) from choroidal blood to support retinal , while fatty acids contribute to photoreceptor membrane renewal. Additionally, the choroid facilitates () transport to the RPE via the receptor STRA6, where it is converted to 11-cis- for photoreceptor function in the . Waste removal from the outer retina is mediated by the choroid through the clearance of metabolic byproducts such as (CO2) and , which diffuse back across into the choriocapillaris for systemic elimination. Photoreceptors produce via aerobic (Warburg effect), which the RPE absorbs and exports to the choroid, preventing accumulation in the subretinal space. The choroid also supports RPE of shed photoreceptor outer segments by providing nutrients that sustain this energy-intensive process, indirectly aiding debris clearance. This bidirectional exchange is enhanced by the choriocapillaris's fenestrations, which permit rapid diffusion of waste products. Specific mechanisms underlying these functions include the high permeability of the choriocapillaris's fenestrated capillaries, which lack a continuous and allow selective passage of plasma components up to the size of small proteins while restricting larger molecules. This structure optimizes nutrient influx and waste efflux. Furthermore, the choroid contributes to and balance in the outer by supporting RPE-mediated (HCO3-) transport; the RPE uses Na+/ cotransporters to move from the subretinal space to choroidal blood, buffering CO2-induced and maintaining stable extracellular for . Diurnal fluctuations in choroidal blood flow and thickness further aid in maintaining and temperature stability.

Thermoregulation and barrier functions

The choroid plays a crucial role in of the through its extensive vascular network, which facilitates heat dissipation from retinal metabolic activity and . The high in the choroid, accounting for approximately 85% of the ocular supply, acts as a to stabilize retinal , preventing overheating during exposure to intense or increased metabolic demands. This modulation occurs via both passive mechanisms, such as convective through the choriocapillaris, and active neural regulation, where sympathetic innervation adjusts choroidal in response to thermal loads, ensuring the maintains a conducive to optimal photoreceptor function. In addition to , the choroid contributes to barrier functions as part of the outer blood-retinal barrier (oBRB), where the fenestrated of the choriocapillaris interacts with and the (RPE) to regulate the passage of molecules and s. Although the choriocapillaris features fenestrations that allow permeability, the overall oBRB integrity relies on tight junctions primarily in the RPE, which prevent unregulated leakage from the choroidal vasculature into the subretinal space. This structure enables the choroid to maintain ion and balance in the , actively pumping ions and water to counteract osmotic pressures and inhibit retinal under normal conditions. The choroidal flow dynamics further support this by facilitating rapid clearance of excess interstitial , ensuring the virtual subretinal space remains free of accumulation. The choroid also supports immune modulation by housing resident immune cells, including macrophages, dendritic cells, and mast cells, which conduct surveillance for pathogens entering via the vascular supply outside the blood- barrier. These cells detect microbial threats and initiate localized inflammatory responses without compromising integrity, serving as a frontline in the . Complementing this, choroidal melanocytes produce , whose content varies with individual pigmentation levels and enhances UV protection by absorbing radiation and stray light to minimize . This scavenging of further reduces oxidative damage to adjacent tissues, linking pigmentation density directly to ocular resilience against environmental stressors.

Development and comparative aspects

Embryonic development

The choroid originates from derived from the , which condenses into layers proximal to the optic cup during early . Melanocytes within the choroid arise from cells, which migrate and differentiate into pigmented cells that contribute to the choroid's pigmentation. This process begins around the fourth week of , when a vascular forms from mesenchymal cells surrounding the optic cup, marking the initial establishment of the choroidal vasculature. By the sixth week of , mesenchymal tissue invades the space between the optic cup and , initiating vascular invasion and the formation of the choriocapillaris layer through . This is primarily driven by (VEGF) secreted by the (RPE), which induces endothelial cell proliferation and capillary formation adjacent to the RPE. Simultaneously, melanoblasts migrate from the into the developing choroid, integrating with the vascular elements to provide pigmentation. The choroidal fissure, an embryonic indentation in the optic cup, closes by the seventh week, a critical event that ensures proper formation of the choroid and prevents , a congenital defect resulting from incomplete closure. The choriocapillaris fully differentiates by the eighth week, supporting the inner choroidal layers, while the complete layering of the choroid— including the Haller's large vessels, Sattler's medium vessels, and choriocapillaris—is achieved by the third month of gestation. During this early phase, the retina remains avascular overall, with its outer layers receiving nutrition from the maturing choroidal vasculature and its inner layers and the lens supplied by the transient hyaloid artery, which regresses as the inner retinal vessels develop in the later stages of gestation and postnatally. Maturation of the choroid continues postnatally, with refinements in vascular density and pigmentation occurring after birth.

Variations across vertebrates

The choroid exhibits significant structural variations across classes, reflecting evolutionary adaptations to diverse visual demands and environmental transitions from to terrestrial habitats. Over approximately 540 million years, the choroid has evolved to support increasing retinal metabolic needs, transitioning from simple diffusive supply in early forms to more complex vascular and supplemental structures in terrestrial lineages. In cartilaginous such as sharks, the choroid features prominent iridescent layers formed by crystals in the choroidal , which enhance low-light vision and contribute to by reflecting ambient light to disrupt silhouettes in environments. Bony (teleosts) show a less developed choriocapillaris compared to terrestrial vertebrates, relying instead on rectangular choroidal glands—vascular networks akin to pseudobranch functions—that concentrate oxygen for nutrition, particularly in species with thick retinas. These glands, often forming a rete mirabile, underscore the choroid's role in adaptations during early in settings. In amphibians, the choroid maintains a primarily diffusive vascular supply to support thin retinas, evolving as a bridge in the aquatic-to-terrestrial transition where ocular demands shifted with emerging aerial . Reptiles exhibit a thicker choroid with the conus papillaris, a vascular projection analogous to the pecten, which supplements choroidal circulation to nourish avascular regions of the and reduce dependence on alone. Birds possess an even more specialized choroid, featuring the —a pigmented, comb-like vascular structure extending into the vitreous humor—that provides direct nutritional support to the metabolically active, avascular , enhancing efficiency in high-acuity for flight and predation. This likely co-evolved with in sauropsids, distinguishing and choroids from those in earlier lineages. Among mammals, the choroid generally mirrors the configuration with high vascular density in the choriocapillaris to sustain outer layers, though pigmentation varies notably; in albinos, reduced in choroidal melanocytes leads to , altering light scattering and contributing to visual pathway anomalies. These interclass differences highlight the choroid's evolutionary , from guanine-based reflectors in forms for enhanced to specialized projections in sauropsids for optimized terrestrial . In bony , melanocytes additionally incorporate crystals forming a tapetal layer within the choroid, which reflects light back through the to improve in dim conditions.

Clinical significance

Associated disorders

The choroid is susceptible to various inflammatory disorders, collectively termed choroiditis, which involve of the choroidal tissue often extending to the as . These conditions can arise from infectious agents, such as , the most common cause of infectious , leading to focal necrotizing lesions in the choroid and . Autoimmune-mediated forms, including serpiginous choroiditis, present as recurrent, asymmetrically bilateral characterized by grayish-white lesions at the margin that progress in a serpentine pattern, resulting from immune-mediated non-perfusion of the choriocapillaris and subsequent of the (RPE) and choroid. Pathophysiologically, these processes cause scarring, photoreceptor damage, and progressive vision loss due to disruption of the choriocapillaris and overlying retinal layers. Vascular disorders of the choroid include (CSCR), a condition involving multifactorial choroidal circulatory disturbances that lead to hyperpermeability and fluid leakage from the choroid into the subretinal space, causing serous typically in the . In age-related (AMD), particularly the neovascular or wet form, (CNV) develops as abnormal, fragile vessels proliferate from the choroid through into the sub-RPE space, driven by overexpression of (VEGF) from dysfunctional RPE cells; this affects the choroid in nearly all wet AMD cases, which comprise about 10-15% of total AMD. These vascular changes compromise the blood-retinal barrier and contribute to , hemorrhage, and central vision impairment. Neoplastic conditions primarily affecting the choroid encompass , the most common primary intraocular , originating from uveal melanocytes in approximately 85-90% of cases. This tumor arises sporadically in the choroid, with an annual incidence of 5-7.5 cases per million individuals, predominantly in those of European descent, and can lead to , vitreous hemorrhage, or to the liver in up to 50% of cases over time. Metastatic tumors to the choroid, often from or primaries, represent another neoplastic category, where tumor emboli lodge in the choroidal vasculature, causing multifocal yellow-white lesions and secondary retinal changes. Congenital anomalies include choroidal coloboma, a developmental defect resulting from incomplete closure of the embryonic optic fissure, leading to a gap-like absence of choroidal tissue, often accompanied by defects in the RPE and , which increases the risk of or visual field defects. In albinism, particularly oculocutaneous forms, hypopigmentation of the choroid due to reduced in the RPE and exposes underlying structures to excessive , heightening phototoxicity and oxidative damage to the choroid and , thereby exacerbating risks of macular and .

Diagnostic and therapeutic approaches

(OCT) is a primary non-invasive imaging modality for evaluating choroidal structure, offering axial resolution of approximately 5-10 μm to assess thickness, subretinal fluid, and choroidal lesions. Enhanced depth imaging OCT (EDI-OCT) and swept-source OCT (SS-OCT) enhance visualization of the choroid by improving penetration depth up to 2-3 mm, enabling precise measurement of choroidal thickness in conditions like () and . angiography (ICGA) provides detailed assessment of choroidal vascular flow and perfusion, particularly useful for detecting hypofluorescent lesions and , and is preferred over () due to its strong protein binding, which allows deeper penetration into choroidal tissues without significant leakage from vessels. Fundus autofluorescence (FAF) imaging aids in evaluating distribution and accumulation in the choroid and , helping to identify patterns in inflammatory or degenerative disorders. , including A-scan and B-scan modes, is employed for measuring tumor dimensions and acoustic properties in choroidal masses like melanomas, with resolutions sufficient for detecting extrascleral extension. assists in differentiating choroidal from vascular abnormalities by highlighting leakage patterns, though it is less effective for deep choroidal structures compared to ICGA. Therapeutic approaches for choroidal conditions target underlying vascular, inflammatory, or neoplastic processes. Intravitreal anti-vascular endothelial growth factor (anti-VEGF) injections, such as ranibizumab, are standard for choroidal neovascularization in AMD and pathologic myopia, inhibiting angiogenesis and reducing leakage with repeated dosing every 4-8 weeks initially. Photodynamic therapy (PDT) using verteporfin, administered intravenously followed by laser activation, selectively occludes abnormal choroidal vessels in wet AMD and polypoidal choroidal vasculopathy, often combined with anti-VEGF for improved outcomes. For inflammatory choroidopathies like Vogt-Koyanagi-Harada disease, systemic corticosteroids serve as first-line therapy to suppress acute inflammation, with transition to immunosuppressants such as cyclosporine or mycophenolate mofetil for steroid-sparing control in chronic cases. Surgical interventions include pars plana vitrectomy to address vitreoretinal complications from choroidal tumors or detachments, often combined with endoresection to remove intraocular masses while preserving the globe. In advanced choroidal melanoma with poor visual prognosis or complications, enucleation remains the definitive treatment to prevent metastasis. Emerging therapies for choroidal melanoma include belzupacap sarotalocan (AU-011), a virus-like drug conjugate in phase 3 clinical trials as of 2025, showing promise for early-stage tumors with vision preservation, and proton beam therapy, which a 2025 meta-analysis confirmed achieves high local control rates (up to 95%) and overall survival benefits.

Historical context

Early descriptions

The choroid, a vascular and pigmented layer of the eye, was first alluded to in texts as a spongy inner distinct from the denser . Around 400 BCE, of Abdera provided the earliest known description and illustration, referring to it as the "chitoon malista somphos" (more spongy ), emphasizing its porous, vascular nature compared to the outer coats. This observation laid the groundwork for later understandings of its membrane-like quality. In the 2nd century AD, of advanced these ideas by systematically delineating the eye's layered structure in his anatomical treatises, identifying the choroid as one of the principal membranes alongside the , , lens capsule, and . He described it as the vascular tunic, highlighting its role in enclosing the eye's internal fluids and its resemblance to a skin-like sheath due to its thin, enveloping form—termed "choroeides" in , evoking a membrane akin to the (the fetal afterbirth sac). This , rooted in "chorioeidēs" (resembling the chorion), reflected its folded, vascular appearance in dissections, though early anatomists often confused it with adjacent structures like the , mistaking its pigmentation for part of the sensory layer. Arabic scholars in the medieval period built upon Galenic foundations, incorporating empirical observations into comprehensive medical encyclopedias. (Ibn Sina, 980–1037 AD) in his detailed the eye's tunics as part of the , viewed as protective and nutritive. During the , anatomical precision improved through dissection and illustration. , in his seminal 1543 work De Humani Corporis Fabrica, depicted the choroid as an integral component of the , illustrating its continuity with the and in cross-sectional views of the eye, thereby correcting some Galenic inaccuracies about layer separations. Gabriele Falloppio, in his 1561 Observationes Anatomicae, expanded on Vesalius's work in ocular . In the 17th and 18th centuries, techniques like vascular injection revealed the choroid's intricate blood supply. , in the 1690s and early 1700s, pioneered wax injections into ocular vessels, vividly demonstrating the choroid's dense capillary network and its role in circulation, which his son later termed the "tunica Ruyschiana" for the complex including the and choriocapillaris. These efforts solidified the choroid's identity as a distinct, highly vascular entity before microscopic advancements.

Modern advancements

In the 19th century, significant progress in choroidal anatomy was made through histological studies, with Karl Bruch identifying and describing in 1844 as a distinct layer separating the choroid from the . This discovery, detailed in Bruch's doctoral thesis, highlighted the membrane's role as a thin, acellular structure essential for choroidal-retinal interactions. Concurrently, histologists such as advanced the understanding of choroidal layering using improved microscopy techniques, delineating the vascular and stromal components in the mid-1800s and establishing the foundational multilayered organization observed in modern histology. The 20th century brought technological innovations that revealed choroidal function and microstructure. In the 1950s, Harold R. Novotny and David L. Alvis pioneered , first demonstrated in 1959, which allowed noninvasive visualization of choroidal blood flow by injecting sodium fluorescein and capturing serial fundus images. This technique revolutionized the assessment of choroidal circulation, enabling the detection of vascular leaks and perfusion abnormalities. By the , electron microscopy provided ultrastructural insights, with studies revealing the fenestrated endothelium of the choriocapillaris, a key feature facilitating nutrient exchange with the , as documented in early examinations of human choroidal tissue. In the 1970s, Anders Bill's quantitative studies in animal models, such as cats, measured choroidal blood flow rates and oxygen extraction, demonstrating high flow volumes—up to 50 times that of the —and its sensitivity to changes. Sohan Hayreh's research during this period further elucidated choroidal hemodynamics, showing limited autoregulation in the choroidal vasculature compared to the retina, with blood flow responding passively to systemic pressure variations in primate and human studies from the 1960s to 1990s. Entering the 21st century, optical coherence tomography (OCT), developed in the early 1990s, evolved to enable in vivo choroidal imaging; enhanced-depth imaging OCT in the 2000s allowed precise thickness mapping, revealing subfoveal choroidal thicknesses averaging 272–354 μm in healthy adults. Genetic research linked choroidal alterations to age-related macular degeneration (AMD), with variants in the complement factor H (CFH) gene, such as Y402H identified in 2005, increasing AMD risk by impairing choroidal neovascularization regulation. Post-2000 advancements in swept-source OCT have quantified dynamic choroidal changes, showing age-related thinning—at a rate of approximately 3 μm per year—and thinning during , up to 10-20 μm in young subjects, aiding in understanding adaptive vascular responses. In the , integration of choroidal imaging with retinal studies via multimodal OCT and has advanced , enabling risk stratification for through combined genetic and vascular biomarkers to tailor therapies like injections; as of 2025, models improve detection of with over 90% accuracy in multimodal datasets.