An ecoregion is defined as a large unit of land or water containing a geographically distinct assemblage of species, communities, and ecological processes that share a large majority of their species composition, dynamics, and environmental conditions, and that interact in ways essential for their sustained viability.[1] This framework emphasizes differentiation from adjacent areas due to barriers to species dispersal or unique abiotic factors such as climate, geology, and soils.[1]The concept of ecoregions gained prominence through the work of the World Wildlife Fund (WWF), which published a comprehensive global classification of terrestrial ecoregions in 2001, delineating 867 such units nested within 14 biomes and 8 biogeographic realms.[1] This system builds on earlier ecological regionalizations but provides a standardized tool for assessing biodiversity patterns at a scale larger than individual habitats yet smaller than continents.[1] A parallel marine classification, the Marine Ecoregions of the World (MEOW), was developed in 2007, identifying 232 ecoregions across 12 realms and 62 provinces to address coastal and shelf biodiversity.[2] A corresponding freshwater classification, the Freshwater Ecoregions of the World (FEOW), was developed in 2008 by WWF and The Nature Conservancy, delineating 426 ecoregions.[3]Ecoregions serve as foundational units for conservation planning, enabling prioritization of areas like the WWF's Global 200 ecoregions, which highlight exceptional biodiversity and endemism for targeted protection efforts.[4] They integrate biotic and abiotic elements to map ecosystems holistically, supporting strategies that maintain ecological integrity amid threats like habitat fragmentation and climate change.[5] In practice, organizations such as the U.S. Environmental Protection Agency apply ecoregion-based approaches to evaluate environmental resources and guide restoration across North America's 15 level I ecoregions.[6]
Definition and Classification
Definition
An ecoregion is a relatively large unit of land or water containing a distinct assemblage of natural communities and species, with boundaries that approximate the aggregated extent of those communities sharing a large majority of their species, dynamics, and environmental conditions.[1] These units are shaped by recurring patterns of ecosystems influenced by major factors such as geology, climate, topography, hydrology, and evolutionary history, which together determine the characteristic biodiversity and ecological processes within the region.[5][7]Key attributes of ecoregions include distinct assemblages of plant and animal species, ecological communities, and interacting environmental factors, often facing similar disturbance regimes like fire or flooding that maintain their structure and function.[1] Boundaries between ecoregions are typically non-abrupt and fuzzy, reflecting gradual ecological transitions rather than sharp demarcations, as species distributions and environmental gradients blend across adjacent areas.[4]Ecoregions differ from related concepts in scale and focus: biomes represent broader, climate-dominated zones that encompass multiple ecoregions, such as the temperate broadleaf forest biome containing various ecoregions adapted to local variations, while ecosystems denote smaller-scale interactions of organisms and their immediate environment within an ecoregion.[1] Typically spanning hundreds of thousands to millions of square kilometers, ecoregions provide a mesoscale framework for understanding global biodiversity patterns, for example, the Congo Basinrainforest ecoregion covering about 1.8 million square kilometers.
Classification Systems
Classification systems for ecoregions employ a variety of approaches to delineate areas of relative ecological homogeneity, ranging from hierarchical frameworks based on environmental drivers to comprehensive global schemes informed by biodiversity patterns. These systems typically integrate abiotic factors such as climate, geology, and hydrology with biotic elements like vegetation and species distributions to create nested hierarchies that facilitate ecological analysis and management.[5]One foundational algorithmic approach is Robert G. Bailey's hierarchical system for North American ecoregions, first mapped in 1976 and expanded in subsequent editions. This framework classifies ecoregions into domains, divisions, provinces, sections, and districts based primarily on macroclimatic zones (using the Köppen-Trewartha system), physiographic features (such as landforms from mountains to plains), and potential natural vegetation (e.g., tundra, forests, or steppes). Bailey's method emphasizes the interaction of these factors to define ecosystem complexes, with mountains often treated separately due to altitudinal zonation, enabling a scalable structure for resource assessment across the continent.[8]In contrast, expert-driven holistic methods, such as James M. Omernik's ecoregion framework developed for the U.S. Environmental Protection Agency, rely on integrative analysis of multiple environmental variables to delineate nested levels (I through IV). Omernik's system identifies broad Level I ecoregions based on similarities in geology, physiography, hydrology, climate, soils, potential natural vegetation, and wildlife, then refines them into finer sublevels through expert judgment and spatial data overlays. This approach, first outlined in 1987 and refined over decades, prioritizes ecological similarity across resource mosaics rather than rigid boundaries, supporting applications in water quality and land management.[9][5]Major global frameworks have emerged to standardize ecoregion delineation worldwide, notably those from the World Wildlife Fund (WWF). The Terrestrial Ecoregions of the World (TEOW), published in 2001, divides the planet's land surface into 867 ecoregions nested within 14 biomes and 8 biogeographic realms, drawing on expert consultations, existing regional classifications, and GIS overlays of vegetation, landforms, and historical biogeography to capture distinct assemblages of species and habitats. Complementing this, the Marine Ecoregions of the World (MEOW, 2007) establishes 232 coastal and shelf ecoregions across 12 realms and 62 provinces, using biogeographic data on species distributions, oceanography, and larval dispersal to define units of relative biodiversity homogeneity. Similarly, the Freshwater Ecoregions of the World (FEOW, 2008) identifies 426 ecoregions aligned with watersheds, primarily based on freshwater fishspecies compositions, endemism patterns, and hydrological barriers, incorporating evolutionary history and regional expert input for conservation prioritization.[10][11][12]Contemporary ecoregion mapping increasingly integrates advanced data sources, including Geographic Information Systems (GIS) for spatial analysis, remote sensing for vegetation and land cover monitoring, and biodiversity databases for species occurrence data. For instance, GIS platforms enable overlaying layers of climatic models, satellite-derived imagery (e.g., Landsat or MODIS), and databases like the Global Biodiversity Information Facility (GBIF) to refine boundaries and assess ecological transitions, as seen in the development of TEOW and updates to national systems. This data fusion enhances accuracy and scalability, allowing for dynamic updates in response to environmental changes.[13][14]
History
Early Developments
The foundational concepts underlying ecoregions trace back to the early 19th century, when Alexander von Humboldt explored the relationships between vegetation distribution and environmental gradients such as altitude, temperature, and humidity during his expeditions in the Americas. Humboldt's observations, detailed in works like Essai sur la géographie des plantes (1807), established the idea of biogeographic zones where plant communities form distinct belts influenced by climatic and physiographic factors, laying the groundwork for later ecological classifications.[15]In the mid-20th century, Russian ecologist Viktor Sukachev advanced these ideas through his development of biogeocenology, formalizing the concept of biogeocenosis—a spatially discrete unit integrating living organisms, their environment, and interactions—in the 1940s and 1950s. This approach emphasized holistic ecosystem units beyond isolated vegetation, influencing global ecological thought by highlighting biotic-abiotic interdependencies. The term "ecoregion" itself was coined in 1962 by Canadian forest ecologist Orie L. Loucks in his classification of forest ecosystems in the Maritime Provinces, where he defined ecoregions as areas with relatively homogeneous climate, physiography, and vegetation suitable for management purposes.[16]Key milestones in the United States included A. W. Kuchler's 1964 map of potential natural vegetation across the conterminous U.S., which delineated approximately 116 vegetation types based primarily on climax plant communities and their environmental controls, providing a vegetation-centric framework for resource planning. Building on this, Robert G. Bailey introduced a more comprehensive ecoregions framework in 1976 for the U.S. Forest Service, dividing the country into 53 provinces (the finest-level ecoregions) within a hierarchy of 9 domains and 31 divisions that integrated climate, physiography, hydrology, soils, and potential vegetation to support ecological land classification and management.Internationally, the UNESCO Man and the Biosphere (MAB) Programme, launched in 1971, spurred global biome mapping efforts, with its 1973 publication on the International Classification and Mapping of Vegetation offering a standardized physiognomic system for delineating major vegetation units at scales useful for biosphere reserves and conservation planning. In the 1980s, the WorldWildlife Fund (WWF) began emphasizing biome-level classifications in its conservation strategies, such as campaigns targeting tropical forests and other major biomes, which paved the way for finer-scale ecoregion delineations by integrating biodiversity patterns with habitat types.Over this period, ecoregion concepts shifted from predominantly vegetation-based mappings, like Kuchler's, to integrated biotic-abiotic models exemplified by Bailey's framework, which incorporated climatic regimes and geomorphic processes to better capture ecosystem dynamics and resilience. This evolution reflected growing recognition that ecoregions must account for multifaceted environmental drivers to inform sustainable land use.
Modern and Recent Advances
In the early 21st century, the World Wildlife Fund (WWF) advanced ecoregion classification through its Terrestrial Ecoregions of the World (TEOW) framework, initially published in 2001, which delineated 867 terrestrial ecoregions based on biogeographic analyses of species distributions and environmental factors.[1] This system was refined in the 2017 RESOLVE Ecoregions dataset, updating the count to 846 ecoregions by incorporating higher-resolution data on vegetation, geology, and climate to better capture ecological boundaries and reduce overlaps from the original mapping.[17] The revised dataset has facilitated integration with the International Union for Conservation of Nature (IUCN) threat assessments, enabling ecoregion-scale evaluations of biodiversity risks such as habitat loss and invasive species, as demonstrated in WWF's Living Planet Reports that overlay IUCN Red List data onto ecoregion maps for global conservation prioritization.[18]In the United States, the Environmental Protection Agency (EPA) refreshed its Level III and IV ecoregion delineations in early 2025, incorporating updated satellite-derived land cover data to refine boundaries for 105 Level III and over 1,000 Level IV units, enhancing applications in water quality monitoring and ecosystem restoration.[19] Concurrently, the Federal Geographic Data Committee (FGDC) released Ecosystem Vegetation Classification (EcoVeg) Version 3.0 in October 2025, aligning with the U.S. National Vegetation Classification (USNVC) standard to introduce a restructured eight-level hierarchy from biomes to local associations, peer-reviewed for consistency and integrated with global typologies like the IUCN Global Ecosystem Typology.[20] This update improves accessibility through interactive maps and open data portals, supporting finer-scale ecoregion mapping for biodiversity inventories and land management.[21]Globally, the One Earth initiative introduced its Bioregions 2020 framework in the early 2020s, defining 185 bioregions as intermediate units between biogeographic realms and ecoregions to emphasize ecological connectivity and human impacts, building on the 846 ecoregions for a hierarchical view of planetary life-support systems.[22] Complementing this, a 2025 global landscape pattern assessment analyzed land use/land cover (LULC) changes from 1992 to 2020 using the HILDA+ dataset, revealing trends such as the expansion and fragmentation of unmanaged grass/shrublands alongside declines in forest and pasture/rangeland areas, informing ecoregion vulnerability to human-induced alterations.[23]Technological integrations have further propelled ecoregion advancements, with AI-driven mapping leveraging Landsat satellite imagery for automated boundary delineation and change detection; for instance, machine learning models applied to multispectral Landsat data since the 2020s enable real-time monitoring of vegetation shifts within ecoregions, improving accuracy over traditional methods.[24] Open-access datasets, such as the Ecoregions 2017 hosted at ecoregions.appspot.com, provide downloadable shapefiles under a CC-BY 4.0 license, fostering collaborative research and integration with platforms like Google Earth Engine for scalable analyses.[25]
Importance
Ecological Role
Ecoregions serve as fundamental units of evolutionary distinctiveness, often encompassing biodiversity hotspots that host unique assemblages of species with high levels of endemism, where a significant proportion of flora and fauna are found nowhere else on Earth. For instance, the WWF's Global 200 ecoregions were selected based on criteria including exceptional species richness and endemism rates.[26][27]Within ecoregion-specific biomes, ecosystem dynamics are regulated by localized processes such as nutrient cycling, where decomposers and plants recycle essential elements like nitrogen and phosphorus, maintaining soil fertility and primary productivity. Hydrology is similarly influenced, with ecoregions shaping water flow patterns through vegetation cover and topography, as seen in forested ecoregions that moderate runoff and groundwater recharge. Carbon sequestration occurs prominently in these units, with biomes like tropical rainforests and temperate forests storing vast amounts of carbon in biomass and soils; for example, the Piedmont ecoregion in the southeastern United States sequesters approximately 3.3 million metric tons of carbon per year through land cover changes.[26][28]Ecoregions enhance ecological resilience by facilitating connectivity across landscapes, supporting metapopulations where subpopulations exchange individuals to buffer against local extinctions. This connectivity promotes gene flow, preserving genetic diversity; in large-scale ecoregions like the boreal forest, migratory pathways enable seasonal movements that sustain population viability and adaptive potential.[26][29]Ecoregions provide critical ecosystem services, including pollination by native insects and birds that sustain plant reproduction and agriculture, soil formation through organic matter accumulation and microbial activity, and habitat provisioning for diverse taxa. These functions are often quantified using species richness indices, such as the Shannon diversity index, indicating robust ecological support for these services in high-biodiversity ecoregions like the Amazon basin.[26][7]
Conservation Applications
Ecoregions serve as fundamental units for designing and implementing conservation strategies, enabling the prioritization of areas that capture the full spectrum of global biodiversity while addressing threats at ecologically meaningful scales. By delineating regions with relatively homogeneous species assemblages and ecological processes, ecoregions facilitate targeted interventions that enhance representation in protected area networks and support policy frameworks aimed at halting biodiversity loss.[1]The World Wildlife Fund (WWF) launched the Global 200 initiative in 2001 to identify priority ecoregions for conservation, selecting 238 terrestrial, freshwater, and marine ecoregions that collectively represent the planet's major habitat types and harbor exceptional biodiversity. This framework prioritizes these areas for action to ensure the preservation of evolutionary processes and species diversity, guiding WWF's ecoregion-based conservation programs worldwide. Although the core list remains foundational, WWF has integrated updates through ongoing assessments, such as incorporating new threat data and aligning with global targets like the UN Sustainable Development Goals.[30]Ecoregions play a key role in aligning protected areas with international biodiversity targets, including the Convention on Biological Diversity's (CBD) 30x30 goal under the Kunming-Montreal Global Biodiversity Framework, which aims to conserve at least 30% of terrestrial and marine areas by 2030 through effective and equitable systems. As of 2024, global protected areas cover approximately 17% of land and 8% of oceans, highlighting gaps addressed through ecoregion-based planning.[31][32] Gap analyses conducted by the International Union for Conservation of Nature (IUCN) and WWF utilize ecoregion boundaries to evaluate representation gaps, identifying underprotected areas where species and habitats are inadequately covered by existing reserves. For instance, these analyses reveal that only a fraction of ecoregions achieve 30% protection, informing expansions of networks like the World Database on Protected Areas to better safeguard biodiversity hotspots.[31]In regional contexts, ecoregion-based planning enhances localized conservation efforts. In the United States, NatureServe and partners like The Nature Conservancy conduct ecoregional assessments to map biodiversity priorities, assess threats, and recommend protected area expansions across western North American ecoregions, such as deserts and grasslands, to maintain ecological connectivity. Similarly, in Europe, the European Environment Agency (EEA) employs biogeographical regions—analogous to ecoregions—as a framework under the EU Habitats Directive to evaluate conservation status and guide the Natura 2000 network, ensuring habitat types are represented proportionally across regions like the Atlantic and Boreal zones.[33][34][35]Monitoring ecoregion health relies on indices that track biodiversity trends and ecosystem integrity. WWF adapts tools like the Living Planet Index, which measures vertebrate population changes, to ecoregion scales in regional reports to detect declines and evaluate conservation outcomes, such as in freshwater ecoregions where pressures from habitat fragmentation are acute. These metrics support adaptive management, allowing conservationists to refine strategies based on verifiable indicators of ecosystem vitality.[36]
Ecoregion Types
Terrestrial Ecoregions
Terrestrial ecoregions encompass the diverse land-based ecosystems of Earth, classified hierarchically by the World Wildlife Fund (WWF) into eight biogeographic realms, such as the Nearctic and Afrotropical, which reflect broad evolutionary histories and continental-scale patterns.[1] These realms are further subdivided into 14 biomes, including tropical and subtropical moist broadleaf forests, temperate grasslands and savannas, and deserts and xeric shrublands, capturing major vegetation and climate zones.[1] In total, this system delineates 867 terrestrial ecoregions, each defined by relatively homogeneous environmental conditions, species assemblages, and ecological processes that distinguish them from neighboring areas.[1]These ecoregions span approximately 149 million square kilometers of Earth's land surface, representing the full extent of habitable terrestrial habitats excluding polar ice caps and barren rock.[37] Biodiversity within terrestrial ecoregions peaks in the tropics, where factors like stable climates and high solar energy support exceptional species richness; for instance, the Neotropical realm's Amazon Rainforest ecoregion hosts over 40,000 plant species and 3,000 vertebrate species, far exceeding temperate zones.[38] In contrast, the Palearctic realm's Siberian Taiga, the world's largest contiguous forest, covers about 12 million square kilometers but features lower diversity with dominant coniferous trees and adapted mammals like moose and wolves.[1]Key characteristics of terrestrial ecoregions include dominant vegetation structures that shape habitat complexity, with forests comprising six of the 14 biomes and covering roughly 31 percent of global land area through dense canopies of broadleaf and needleleaf trees.[17] Soil types vary distinctly by biome, such as the nutrient-poor, acidic podzols in boreal forests or the fertile, clay-rich alfisols in temperate deciduous woodlands, influencing nutrientcycling and plant adaptations.[1] Faunal assemblages are biome-specific, featuring high endemism in tropical realms—e.g., primates and felids in Afrotropical savannas—while arid ecoregions support specialized reptiles and ungulates resilient to water scarcity.[39]
Marine Ecoregions
Marine ecoregions are delineated through the Marine Ecoregions of the World (MEOW) framework, a hierarchical biogeographic classification system developed to capture patterns of marine biodiversity in coastal and shelf areas. This system organizes the world's marine environments into 12 realms, 62 provinces, and 232 distinct ecoregions, focusing primarily on waters shallower than 200 meters where most coastal and shelf ecosystems occur.[11] The framework emphasizes both coastal zones, including intertidal and benthic habitats, and the overlying pelagic zones within shelf areas, providing a nested structure for analyzing ecological similarity and distinctiveness.[11]Key characteristics of marine ecoregions are shaped by oceanographic processes such as currents, salinity gradients, and upwelling, which drive nutrient distribution, temperature variations, and species assemblages. Ocean currents transport larvae and influence connectivity between regions, while salinity variations—often resulting from freshwater inflows or evaporation—create unique hydrographic conditions that support specialized communities. Upwelling brings nutrient-rich deep waters to the surface, fostering high productivity in certain ecoregions and enabling diverse food webs. These factors distinguish marine ecoregions from terrestrial ones by prioritizing dynamic water mass movements over static land-based features like soil or topography.[11]Representative examples illustrate these influences. The Coral Triangle, located within the Central Indo-Pacific realm, exemplifies a high-diversity hotspot driven by the Indonesian Throughflow—a major current system that mixes Pacific and Indian Ocean waters, promoting exceptional coral and fish diversity through enhanced nutrient upwelling and larval dispersal. In contrast, the California Current ecoregion in the Cold Temperate Northeast Pacific province (part of the Temperate Northern Pacific realm) features seasonal upwelling along temperate shelves, supporting productive fisheries and kelp forests adapted to cooler, nutrient-laden waters.[40] Similarly, the Agulhas Bank ecoregion in the Agulhas province (Temperate Southern Africa realm) is influenced by the swift Agulhas Current, which generates intermittent upwelling and eddies, sustaining subtropical to temperate biodiversity in a transition zone between tropical and polar influences.[41]These ecoregions collectively span the global ocean, which covers approximately 70% of Earth's surface, though MEOW specifically targets the biologically rich coastal and continental shelf areas representing about 7.7% of total ocean extent. This distribution ensures comprehensive coverage of nearshore habitats critical for global marine productivity, from polar ice-influenced realms to equatorial tropical systems.[11]Conservation priorities for marine ecoregions highlight their role in protecting biodiversity hotspots, with many exhibiting high levels of endemism due to isolation by currents or bathymetric barriers. The MEOW framework facilitates integration with marine protected areas (MPAs) by enabling systematic gap analyses and representativeness assessments, allowing planners to prioritize underrepresented ecoregions for protection and enhance global MPA networks. For instance, it has been used to evaluate how MPAs align with ecoregional boundaries, promoting more effective ecosystem-based management.[11]
Freshwater Ecoregions
Freshwater ecoregions represent distinct biogeographic units within inland aquatic systems, delineated to capture patterns of freshwater biodiversity and support conservation efforts. The primary framework for their classification is the Freshwater Ecoregions of the World (FEOW), developed by the World Wildlife Fund and collaborators, which identifies 426 ecoregions spanning nearly all non-marine areas of the globe. These ecoregions are organized into 12 major habitat types, including large lakes, large river deltas, montane freshwaters, xeric freshwaters and endorheic basins, temperate coastal rivers, temperate upland rivers, temperate floodplain rivers and wetland complexes, tropical and subtropical coastal rivers, tropical and subtropical upland rivers, tropical and subtropical floodplain rivers and wetland complexes, polar freshwaters, and oceanic islands.[42][43] This classification emphasizes ecological and evolutionary processes unique to freshwater systems, distributed across major biogeographic realms such as the Nearctic, Afrotropical, and Indomalaya.These ecoregions are primarily defined by variations in hydrology—such as flow regimes, seasonal flooding, and water residence times—along with water chemistry factors like pH, salinity, temperature, and nutrient levels, which together shape assemblages of aquatic organisms. Endemic species richness often serves as a key indicator of ecoregional boundaries, reflecting historical isolation and adaptation. For instance, the Laurentian Great Lakes ecoregion (ID 116) in the Nearctic realm encompasses the watersheds of Lakes Superior, Michigan, Huron, Erie, and Ontario, holding over 20% of the world's surface freshwater across 245,000 km² of lake area. Its oligotrophic waters, influenced by glacial origins and underlying acidic Canadian Shield bedrock, support a diverse cold-water fish community, including endemic cisco species flocks such as the bloater (Coregonus hoyi) and the extinct blue pike (Sander vitreus glaucus).[44]Globally, freshwater ecoregions are concentrated within major river basins, where they form complex networks of interconnected habitats that facilitate species dispersal and ecological processes. In the Indomalaya realm, the Mekong Delta ecoregion (ID 729) exemplifies a large river deltahabitat, encompassing the lower Mekong River and tributaries like the Tonle Sap, with extensive peat swamp forests and seasonal flooding that sustains high biodiversity. This area features lowland tropical hydrology with bimodal flood pulses, supporting endemic fishes such as Akysis similis and Hemibagrus spilopterus amid diverse wetland complexes. Similarly, in the Afrotropical realm, the Upper Congo ecoregion (ID 540) within the vast Congo Basin highlights tropical upland river characteristics, with the Lualaba River's slow-flowing reaches and rapids transitioning from savanna to equatorial forest, fostering endemics like Barbus papilio and Chiloglanis marlieri in its riverine and swamp habitats.[45][46]A critical aspect of freshwater ecoregion dynamics is connectivity, which enables migration and gene flow among aquatic species but is increasingly threatened by anthropogenic barriers. Dams fragment habitats by blocking longitudinal river connectivity, altering flow regimes and sediment transport essential for downstream ecosystems. Global analyses indicate that only 37% of rivers longer than 1,000 km remain free-flowing over their entire length, with dams being the primary cause of this loss, underscoring the vulnerability of many ecoregions to such alterations.[47]
Challenges
Climate Change Effects
Climate change is driving significant shifts in the distribution and composition of ecoregions worldwide, primarily through biome migrations as species and ecosystems respond to warming temperatures and altered precipitation patterns. A seminal study identified 89 globally exceptional ecoregions, noting that even under moderate warming scenarios, their entire ranges would experience extreme monthly temperatures exceeding historical norms with less than 2°C of local warming by 2070.[48] Recent projections using Coupled Model Intercomparison Project Phase 6 (CMIP6) models indicate more pronounced biome shifts than earlier estimates in South America, with widespread novel climate conditions emerging across terrestrial biomes by mid-century under high-emission scenarios.[49] These migrations are expected to accelerate in mountainous and polar regions, where velocity of climate change— the rate at which species must move to track suitable conditions—could exceed natural dispersal capabilities.[50]Vulnerability hotspots are particularly evident in Arctic and tropical ecoregions, where warming facilitates biological invasions that disrupt native assemblages. A 2023 analysis found that climate change alters invasion vulnerability across global ecoregions, with Arctic tundra facing heightened risks from non-native species establishment due to expanded thermal tolerances and reduced winter barriers.[51] In North America, projections highlight potential refugia—areas of relative climatic stability—such as certain high-elevation and coastal areas, which may persist as analogs to current conditions amid broader ecoregion displacement.[52] These refugia could serve as critical havens for biodiversity, though their efficacy depends on connectivity and protection from secondary stressors.Ecoregion functionalities are also compromised, with notable impacts on ecosystem services like carbon sequestration and water regulation. In forest ecoregions, climate-induced stressors such as droughts and fires have led to reduced carbon storage, with western U.S. forests showing declines in live biomass carbon across multiple ecoregions since the early 2000s.[53] Globally, up to 32.6% of forest ecoregions are projected to be highly vulnerable under high-emission pathways, exacerbating net carbon losses.[54] In North American freshwater ecoregions, altered hydrology, including shifts in streamflow timing and magnitude, disrupts nutrient cycling and habitat availability in riverine systems.[55]Recent assessments underscore the cascading effects on conservationinfrastructure. A 2021 study revealed that climate-driven ecoregion displacements could undermine protected area targets, with 4.6% of global protected lands experiencing novel climates by the mid- to late 21st century under 2°C warming, reducing their representativeness of original ecoregion types.[56] In the Kangchenjunga Landscape of the Hindu Kush Himalaya (HKH) region, UNFCCC reports from the early 2020s document rapid ecoregion shifts, including upward migration of montane forests and degradation of alpine meadows.[57] These changes highlight the need for dynamic conservation strategies that account for projected displacements, particularly in regions like the HKH where they threaten water security for over 240 million people downstream.
Human Impacts and Management
Human activities have profoundly altered ecoregions through habitat loss, primarily driven by deforestation, agriculture, and urbanization. In tropical moist forests, an estimated 17% of the original area has been lost since 1990, with over 420 million hectares of global forest cover converted worldwide by 2020, more than 90% of which occurred in tropical regions.[58][59] This loss disrupts ecosystem structure and reduces biodiversity, often converting biodiverse habitats into monoculture plantations or urban developments.Habitat fragmentation exacerbates these effects by creating barriers to animal migration and gene flow in terrestrial ecoregions. Approximately 70% of remaining global forest is located within 1 km of an edge, exposing it to edge effects like increased human access and altered microclimates that hinder wildlife movement.[60] Fragmented landscapes also facilitate the spread of invasive species, as disturbed patches provide entry points and reduced native competition allow invasives to establish and proliferate, further degrading native biodiversity.[61]Pollution from industrial emissions, agricultural runoff, and urban waste introduces contaminants that acidify soils and water bodies, impairing ecosystem functions across ecoregions. Atmospheric deposition of nitrogen and sulfur, for instance, leads to eutrophication and toxicity in forests and wetlands, reducing plant growth and altering species composition.[62]Overexploitation, including overfishing in marine ecoregions and excessive logging or hunting in terrestrial ones, depletes key species at rates exceeding natural replenishment. Examples include the collapse of fish stocks in coastal ecoregions due to industrial trawling and the decline of large mammals in savanna ecoregions from poaching, threatening trophic balances.[63]Management strategies emphasize ecoregion-scale restoration to mitigate these impacts and promote resilience. The EcoVeg approach, a vegetation-based classification system, supports adaptive management by mapping ecosystem types for targeted restoration efforts, such as replanting native species in fragmented U.S. landscapes to reconnect habitats.[64] International agreements like the Ramsar Convention provide frameworks for wetland ecoregion protection, designating sites for conservation and wise use to maintain hydrological and biodiversity functions through regulated land practices.[65]Ongoing monitoring relies on global land use and land cover (LULC) datasets to track human-induced changes at fine resolutions. Updated 2025 datasets, such as the 10 m global land cover reference aligned with Sentinel-2 imagery, enable detection of habitat alterations and evaluation of management effectiveness across ecoregions.[66] These tools support data-driven interventions, like prioritizing anti-deforestation measures in high-risk tropical areas.