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Gray treefrog

The gray treefrog (Dryophytes versicolor), also known as the eastern gray treefrog, is a small, arboreal species of frog in the family Hylidae, native to eastern North America and distinguished by its remarkable ability to change skin color from gray or brown to green for camouflage against tree bark or foliage.^[1] Adults typically measure 32–60 mm (1.25–2.4 in) in length, with males slightly smaller than females, and feature rough, warty skin, prominent white spots beneath each eye, large adhesive toe pads for climbing, and hidden bright yellow or orange mottling on the undersides of their hind legs that serves as a warning to predators.^[2] It forms a cryptic species complex with the morphologically identical Cope's gray treefrog (D. chrysoscelis), the primary differences being D. versicolor's tetraploid chromosome number (4n=48) compared to the diploid D. chrysoscelis (2n=24), along with slower, more melodic advertisement calls in the former due to larger larynx size from polyploidy.^[3]^[4] Gray treefrogs primarily inhabit deciduous and mixed woodlands, swamps, forest edges, and even suburban areas with ample trees and nearby water, often climbing to heights of up to 20 m in the canopy during the non-breeding season.^[2] Their geographic range spans from southeastern Canada (including southern Quebec, Ontario, and New Brunswick) southward through the northeastern and midwestern United States to northern Florida and central Texas, with a preference for humid, temperate climates.^[1]^[2] Nocturnal and arboreal, they spend days concealed in tree crevices or under bark and emerge at night to forage on small invertebrates, including insects like beetles, moths, and spiders, as well as snails and slugs, using their extendable tongues and sticky digits to capture prey.^[1] Tadpoles are herbivorous, consuming algae and organic detritus in aquatic environments.^[1] Reproduction occurs from late April to August in temporary or semi-permanent pools, ponds, and flooded areas, where males perch on vegetation 0.5–2 m above water and emit a loud, short trill—lasting about 3 seconds at 16–34 pulses per second—for up to 4 hours nightly to defend territories and attract females.^[1]^[3] Females deposit small clutches of 10–40 eggs (up to 2,000 total per season) in gelatinous masses attached to submerged vegetation, which hatch into tadpoles within 3–7 days; metamorphosis to froglets takes 45–65 days, with sexual maturity reached at 2 years.^[1]^[3] The species complex's evolutionary origin traces to a single autopolyploid genome duplication event in D. versicolor from an ancestral D. chrysoscelis-like population approximately 100,000–426,000 years ago, with evidence of ongoing hybridization in overlap zones contributing to genetic diversity.^[4] Although classified as Least Concern globally by the IUCN due to its wide distribution and large population, gray treefrogs experience localized declines from habitat fragmentation, road mortality, and pollutants like the pesticide carbaryl, which can cause 10–90% tadpole mortality.^[2] They require at least 60 m of undisturbed terrestrial buffer around breeding wetlands for survival, underscoring the need for habitat preservation in forested and riparian zones.^[2] In some regions, such as parts of North Carolina, D. versicolor is considered significantly rare, while overall trends remain stable with lifespans of 7–9 years in the wild.^[3]^[5]

Taxonomy and identification

Scientific classification

The gray treefrog, formally known as Dryophytes versicolor (LeConte, 1825), belongs to the family Hylidae, a diverse group of arboreal frogs primarily distributed across the Americas. In 2016, Duellman et al. revised the taxonomy, transferring many North American species from the genus Hyla to Dryophytes based on phylogenetic analyses.^[6] This species represents a tetraploid member of the North American treefrog clade, distinguished genetically from its diploid relatives through polyploid speciation events that occurred multiple times during the Pleistocene epoch.^[5]^[7]

Rank	Classification
Kingdom	Animalia
Phylum	Chordata
Class	Amphibia
Order	Anura
Family	Hylidae
Genus	Dryophytes
Species	versicolor

The binomial name Dryophytes versicolor reflects its taxonomic history, with the genus Dryophytes derived from Greek roots drys (tree) and phyton (plant), emphasizing its arboreal lifestyle, while the specific epithet versicolor originates from Latin versi (various or turned) and color (color), alluding to the frog's ability to change skin coloration.^[9]^[10] Historical synonyms include Hyla versicolor LeConte, 1825 (the basionym), Hyla phaeocrypta Cope, 1889, Hyla richardii Baird, 1854, and earlier designations such as Hyla verrucosa Daudin in Sonnini de Manoncourt and Latreille, 1801.^[8]^[2] Genetically, D. versicolor is characterized by a tetraploid chromosome number of 2n=48, twice that of its close relative Dryophytes chrysoscelis (2n=24), which arose through autopolyploidy via genome duplication from a D. chrysoscelis-like ancestor; this ploidy difference prevents interbreeding despite morphological similarities.^[7]^[11] Phylogenetically, the species is nested within the subfamily Hylinae of Hylidae, forming part of a cryptic species complex with D. chrysoscelis in eastern North America, where it occupies a derived position in the treefrog radiation supported by mitochondrial and nuclear DNA analyses.^[5]^[12]

Distinction from similar species

The gray treefrog (Dryophytes versicolor) is morphologically cryptic with its close relative, Cope's gray treefrog (Dryophytes chrysoscelis), and reliable field identification often relies on acoustic and genetic characteristics rather than external appearance alone.^[13] Both species exhibit similar gray to green dorsal coloration with lichen-like patterns, warty skin texture, and bright yellow flash colors on the concealed surfaces of the thighs and groin, though some observations suggest the thigh pigmentation in D. versicolor may appear slightly less vivid.^[14] Subtle morphological clues include a marginally rougher dorsal skin texture in D. versicolor compared to the smoother skin of D. chrysoscelis, as well as a slightly larger average snout-vent length (up to 60 mm versus 50 mm), but these traits show considerable overlap and are not diagnostic without confirmation.^[15] The most reliable distinction in the field is the male advertisement call, which serves as a reproductive isolating mechanism. The gray treefrog produces a slow, musical trill lasting 2–3 seconds at a pulse rate of 15–35 pulses per second, sounding bird-like and resonant, while Cope's gray treefrog emits a faster, buzzier trill at 45–70 pulses per second.^[9] These pulse rates vary with temperature—increasing by about 1–2 pulses per second per degree Celsius—but show no overlap between species at equivalent temperatures, allowing acoustic differentiation even in sympatric areas.^[16] Genetically, the species differ in ploidy level: D. versicolor is tetraploid with 2n=48 chromosomes, whereas D. chrysoscelis is diploid with 2n=24 chromosomes, a distinction confirmed through cytogenetic analysis or nucleolar counts in tissue samples.^[11] In research settings, genetic and acoustic surveys, including playback experiments and chromosomal assays, are employed to verify identifications, particularly in zones of range overlap.^[13] Their ranges overlap sympatrically in the southern portions of the gray treefrog's distribution, such as parts of the central and southeastern United States, but become allopatric northward where D. versicolor predominates in the Northeast and Midwest, while D. chrysoscelis is more common in the Southeast.^[17] This geographic pattern aids preliminary identification but requires corroboration via calls or genetics in transitional zones.^[16]

Physical description

Morphology

The gray treefrog (Hyla versicolor) is a medium-sized anuran with adults typically measuring 32–65 mm in snout-vent length (1.25–2.5 inches), with females generally larger than males (32–51 mm for males and 33–60 mm for females).^[2]^[7] The body exhibits a robust, arboreal build adapted for climbing, featuring expanded disc-like adhesive pads on the tips of all digits that secrete mucus to facilitate attachment to vertical surfaces.^[18]^[7] The skin on the dorsal surface is warty and granular, providing a textured appearance, while the ventral surface is smooth; unlike toads, gray treefrogs lack parotoid glands behind the eyes.^[7] The head is broad with large eyes featuring horizontal pupils, and a prominent white spot beneath each eye, and the limbs are well-developed, with long hind legs suited for jumping and partial webbing between the toes of the hind feet to aid in swimming.^[19]^[1]^[2] Sexual dimorphism is evident in the throat region, where males possess paired, spherical vocal sacs used for amplifying calls and a darker throat with blackish spots, features absent in females.^[2] Internally, the species has simple lungs for pulmonary respiration but relies significantly on cutaneous respiration through the moist skin, which supplements oxygen uptake especially during periods of inactivity or hibernation.^[7]^[20]

Coloration and variation

The gray treefrog (Hyla versicolor) exhibits remarkable variation in coloration, ranging from light gray to vibrant green or brown, enabling effective camouflage against diverse backgrounds such as tree bark or foliage.^[7] This variability arises from the activity of dermal chromatophores, including melanophores that contain melanin for darker tones, iridophores that reflect light to produce blue and green hues, and xanthophores that contribute yellow pigments, allowing the frog to adjust its appearance through pigment dispersion or aggregation.^[21] Color changes occur relatively rapidly, often within seconds to minutes, though full adjustments can take up to several hours depending on environmental conditions, distinguishing this process from the near-instantaneous shifts seen in chameleons.^[22]^[23] Dorsal patterns typically feature mottled or blotched markings in darker shades, enhancing blending with textured surfaces, while the concealed undersides of the hind legs, including the thighs and groin, display bright yellow to orange mottling that remains hidden during rest but can be revealed suddenly.^[7]^[1] These patterns primarily serve a camouflage function, allowing the frog to match arboreal substrates and evade visual predators by reducing detection through background resemblance.^[23] Ontogenetically, juveniles often appear brighter green shortly after metamorphosis, facilitating integration into leafy environments, whereas adults tend toward duller grays or browns, with minimal sexual dimorphism in coloration beyond subtle throat darkening in breeding males.^[7]^[13] Physiological triggers for color variation include background contrast, temperature, humidity, and stress, with the frog darkening on cooler, darker, or humid substrates and lightening on warmer, brighter ones to optimize thermoregulation and concealment.^[24]^[22] For instance, exposure to a dark background prompts melanophore expansion, while elevated temperatures accelerate pigment contraction for lighter tones.^[25] Stress hormones like norepinephrine further modulate these responses, enhancing adaptive shifts during environmental challenges.^[21]

Distribution and habitat

Geographic range

The gray treefrog (Dryophytes versicolor, formerly Hyla versicolor) is native to eastern North America, with its range spanning southeastern Canada from southern Manitoba and New Brunswick southward through the northeastern and south-central United States to eastern Texas, northern Florida, and westward to the Great Plains including North Dakota and eastern Oklahoma.^[5]^[7] This distribution covers approximately 2.5 million square kilometers across five Canadian provinces and 33 U.S. states, though populations are discontinuous in certain prairie regions where suitable wooded habitats are fragmented.^[5] The species' range has remained largely stable since its formal description in 1825, with no evidence of major contractions but potential for minor local extirpations due to habitat loss or isolation in peripheral areas.^[5]^[9] Recent observations indicate possible expansions in the Maritime provinces of Canada, potentially linked to human-mediated dispersal.^[26] The species occupies diverse forested elevations up to mid-highland zones in the Appalachian Mountains.^[27] In the central and southern portions of its range, the gray treefrog exhibits sympatry with the closely related Cope's gray treefrog (D. chrysoscelis), particularly in areas from the Midwest to the Southeast, where the two species co-occur but are distinguished by genetic, chromosomal, and vocal differences.^[5]^[28] This overlap spans regions like Missouri, Arkansas, and Virginia, contributing to challenges in historical mapping but not affecting overall range stability.^[29]

Habitat preferences

The gray treefrog (Dryophytes versicolor) primarily inhabits deciduous and mixed forests, including oak-hickory woodlands, where it utilizes arboreal microhabitats such as tree trunks, branches, and foliage for refuge and foraging.^[30] These frogs are highly arboreal, often found in the canopy or on bark during non-breeding periods, and they adapt to woodland edges, farmlands, and suburban areas with sufficient tree cover.^[31] In suburban environments, they tolerate habitat fragmentation provided that connected wetlands and forested buffers persist to support movement between sites.^[32] For breeding, gray treefrogs select temporary or permanent wetlands, such as woodland ponds, swamps, roadside ditches, flooded fields, and sloughs, with a strong preference for fishless waters to minimize predation on eggs and larvae.^[33] They favor vegetated edges of these wetlands, often using emergent vegetation or adjacent shrubs for calling and oviposition sites.^[34] Reproductive success is notably higher in fishless habitats compared to those with predatory fish.^[35] During the day, gray treefrogs seek refuges in tree crevices, under loose bark, or in small tree cavities, particularly in species like white oak (Quercus alba), to avoid desiccation and predators.^[30] At night, they emerge for arboreal foraging, typically limiting activity to adjacent trees within an estimated home range of about 530 m² and average nightly movements of 6-53 m.^[30] Seasonally, they shift to higher canopy positions in summer under moist conditions, while overwintering from late fall through spring under leaf litter, in shallow soil burrows, or occasionally on elevated branches near the forest floor.^[30] These terrestrial activities occur within 200-300 m of breeding wetlands, emphasizing the need for forested buffers to connect aquatic and upland habitats.^[30]^[34]

Behavior and ecology

Activity patterns

The gray treefrog exhibits a predominantly nocturnal and crepuscular activity pattern, remaining inactive during daylight hours when it seeks shelter in shaded arboreal retreats such as tree cavities, leaf axils, or bark crevices to avoid desiccation and predation.^[30] This diurnal inactivity contrasts with heightened mobility at dusk and dawn, as well as throughout the night, when individuals emerge to forage and navigate their habitat.^[1] Seasonally, gray treefrogs are active from late March or April through October, aligning with warmer temperatures and insect availability, before entering hibernation from November to March.^[33] During hibernation, they burrow into forest debris, leaf litter, or shallow soil depressions beneath logs, where they tolerate extracellular freezing of body fluids down to -6 to -8°C, facilitated by the accumulation of glycerol as a cryoprotectant that prevents ice crystal damage to cells.^[36] This freeze-tolerant strategy allows survival in subzero conditions without significant metabolic activity, with frogs emerging in spring as soil temperatures rise above freezing.^[37] Adult gray treefrogs maintain localized movement patterns, typically foraging within small home ranges of approximately 500 m², often confined to a few adjacent trees and covering average linear distances of 50-80 m during active periods, though post-breeding migrations to overwintering sites can extend up to several hundred meters from breeding areas.^[30] Juveniles, upon metamorphosis, rapidly disperse from natal ponds—often within hours to days—climbing into low vegetation and trees to establish terrestrial habits, with some individuals traveling up to 800 m from the water. Activity levels are strongly influenced by environmental conditions, with increased movement and foraging occurring on warm, humid nights when temperatures exceed 15°C and relative humidity is high, conditions that enhance mobility while minimizing water loss.^[38] In contrast, cooler or drier weather suppresses nocturnal excursions, leading to prolonged retreats.^[30]

Diet and feeding

The gray treefrog (Hyla versicolor) is a carnivorous predator as an adult, primarily consuming insects such as ants (comprising 41.2% of the diet), beetles (29.6%), moths, flies, and crickets, along with occasional spiders and other small arthropods.^[39] These prey items reflect a generalist foraging approach in terrestrial habitats, where frogs exploit abundant woodland invertebrates.^[40] Adults employ a sit-and-wait ambush strategy from vegetation perches, relying on visual cues to detect movement and projecting their adhesive tongue to capture prey.^[9] Feeding is predominantly nocturnal, with individuals often opportunistically gathering near artificial lights that attract insects, such as porch lamps in suburban and forested areas.^[41] Stomach content mass varies seasonally and by location, increasing with distance from breeding ponds—up to higher volumes in arboreal refugia compared to pond edges—indicating peak intake during non-breeding periods when frogs forage farther afield.^[40] Tadpoles exhibit an ontogenetic dietary shift, adopting a herbivorous and detritivorous lifestyle by grazing on algae, periphyton, and organic detritus in pond substrates rather than pursuing animal prey.^[7] This transition underscores the species' role in aquatic and terrestrial food webs, where adults help control pest insect populations, including mosquitoes, gnats, and flies, in both forest and suburban ecosystems.^[7]

Predation and defense

The gray treefrog faces predation across its life stages from a variety of vertebrates and invertebrates. Adults are preyed upon by birds such as wading species and herons, snakes including ribbon snakes, garter snakes, and watersnakes, small mammals, and larger frogs like bullfrogs.^[7]^[33] Tadpoles are vulnerable to fish, predaceous aquatic insects, salamander larvae, and other amphibians, while eggs and newly metamorphosed froglets may be consumed by fishing spiders, bullfrogs, and green frogs.^[33]^[7] To counter these threats, gray treefrogs employ a suite of morphological and behavioral defenses. Their cryptic coloration allows them to blend seamlessly with tree bark and foliage, often remaining immobile during the day to avoid detection.^[7] When disturbed, adults reveal bright yellow or orange flash colors on their hidden inner thighs during leaps, serving as a deimatic display to startle predators and facilitate escape.^[42] Additionally, their skin produces noxious secretions that render them unpalatable and irritating to predators like shrews, providing a chemical deterrent.^[9] Behavioral adaptations further enhance survival. As nocturnal animals, gray treefrogs minimize exposure to diurnal predators by being active primarily at night and calling after dusk.^[7] They adopt a freezing posture when threatened and rely on rapid jumping to evade capture, often combining this with the flash display for maximum effect.^[42] Tadpoles detect predators via chemosensory cues, reducing activity and releasing alarm substances to alert conspecifics.^[7] Eggs and tadpoles face heightened risks in shallow, temporary water bodies like vernal pools, where predation is intense and desiccation can occur if pools evaporate prematurely; adults mitigate this by selecting sites with low fish presence.^[11] Overall survival reflects these pressures: adults exhibit relatively high longevity, with records exceeding 7 years in the wild and captivity, while tadpole mortality often reaches 70–90% due to predation, exacerbated by stressors that can elevate lethality.^[7]^[43]^[44]

Reproduction

Breeding biology

The breeding season of the gray treefrog (Dryophytes versicolor) typically spans from late April to August, varying by region and weather conditions, initiated by evening air temperatures exceeding 15°C and accompanied by rainfall, with active chorusing lasting 4–8 weeks at individual sites.^[7]^[31] Breeding occurs primarily in fishless or low-predation aquatic habitats such as temporary ponds, swamps, flooded fields, and woodland pools, where females deposit eggs in shallow water attached to submerged or floating vegetation in communal masses.^[33]^[2] Females produce one or more clutches per season, totaling 1,000–2,000 eggs or more, with larger individuals or those in superior body condition yielding up to 2,217 eggs; these are laid in multiple small clusters of 10–40 eggs each, often overlapping with those of other females.^[45]^[7] Sexual maturity is reached by females at 2–3 years of age, while males breed annually after 1 year, enabling repeated participation in choruses across seasons.^[7]^[45] Male chorusing synchronizes breeding activity through acoustic stimulation, which influences hormonal levels such as progesterone and prostaglandins in females, promoting ovarian development and phonotactic responses to calls.^[46]^[47]

Mating behaviors

Males of the gray treefrog (Dryophytes versicolor) primarily advertise for mates through a slow trill call, consisting of a series of pulses delivered at a rate of 16–34 pulses per second, which serves as the species' advertisement call to attract females during breeding choruses.^[48] These calls are produced from perches typically 0.5–2 m above the water surface in vegetation surrounding breeding ponds, allowing for effective sound propagation in the chorus environment.^[49] Males aggregate into choruses at these sites, with call characteristics such as duration and effort adjusted in response to neighboring males to maintain competitive vocal output.^[50] In the mating process, males arrive at breeding sites earlier than females, often defending specific calling sites within the chorus to establish territory and increase visibility to approaching mates.^[51] Gravid females, arriving later, exhibit phonotaxis by orienting toward and moving to the choruses based on the collective advertisement calls, perching near pond edges to evaluate potential mates acoustically before selecting one.^[50] Male-male competition occurs both acoustically and physically within dense choruses, where overlapping calls create interference that can reduce a male's attractiveness to females.^[51] Interactions may escalate from vocal exchanges to physical wrestling over calling sites, with larger or heavier males typically dominating these contests and securing better positions.^[52] This aggression helps maintain spacing and priority access to receptive females.^[51] Females exercise mate choice primarily through preferences for acoustic traits in advertisement calls, favoring longer calls (e.g., those with 18–27 pulses) over shorter ones, as these indicate higher male body size and overall health or genetic quality.^[53] In multi-male encounters, females often approach choruses and select among several calling males, sometimes encountering satellite males attempting to intercept, though primary choice is based on call duration and effort.^[50] Once a female selects a male, mating proceeds via axillary amplexus, where the male grasps the female around the torso to stimulate egg release, with females potentially engaging in multiple amplexus events across the breeding season if not fully depleted of eggs.^[50]

Larval development

The eggs of the gray treefrog (Dryophytes versicolor) are deposited in small gelatinous masses containing 10–40 eggs each, which typically float on the water surface or loosely attach to submerged vegetation in quiet pools. These masses are light-jellied and may sink slightly as development progresses, with hatching occurring 3–7 days after oviposition, depending on water temperature.^[1]^[2]^[54] Newly hatched tadpoles are herbivorous, primarily consuming algae, diatoms, and organic detritus scraped from substrates, and feature a dental formula of 2/3 that supports this feeding mode. Their morphology includes a rounded body, dorsolaterally positioned eyes, a sinistral spiracle, and a prominent tail fin—often with red-orange pigmentation and black blotches—for propulsion through water, enabling effective swimming in lentic habitats. Tadpoles grow to a maximum total length of approximately 50 mm before metamorphosis.^[55]^[2]^[56] Larval growth spans 6–8 weeks (45–65 days post-hatching), culminating in metamorphosis when individuals reach 15–25 mm in snout-vent length; warmer water temperatures accelerate this process, potentially shortening the duration. Throughout this stage, tadpoles form schools in shallow pond margins, where they are highly susceptible to mortality from habitat desiccation in temporary wetlands. Tadpoles face predation from aquatic invertebrates and fish, though some may employ unpalatability or behavioral avoidance.^[7]^[1]^[9] Metamorphosis involves the emergence of forelimbs around Gosner stage 42, concurrent development of functional lungs for aerial respiration, and resorption of the tail and gills. Emerging froglets then leave the water, often climbing emergent vegetation to avoid terrestrial predators and desiccation while completing the transition to a terrestrial lifestyle.^[55]^[2]

Interactions

Gray treefrogs exhibit social behaviors primarily during the breeding season, where males aggregate into choruses to collectively attract females. These choruses consist of calling males spaced in vegetation near breeding ponds, with individuals adjusting their vocal output based on group size and proximity to neighbors. In small groups of two males, individuals tend to avoid overlapping calls, but this avoidance diminishes in larger choruses of up to eight or more, where call overlap increases due to the complexity of interactions.^[51] Such dynamics facilitate mutual attraction by amplifying the overall acoustic signal, making the breeding site more detectable to females from a distance.^[7] Within choruses, alternative mating tactics emerge, including the presence of satellite males that do not call but position themselves near dominant calling males to intercept approaching females. Satellite males exploit the calling efforts of others, potentially increasing their mating success without the energetic costs of advertisement calls. This behavior is more common in dense choruses, where competition for calling sites is high.^[7] Outside of breeding, gray treefrogs show loose aggregations centered around foraging opportunities, such as porch lights or street lamps that attract insects. Multiple individuals may congregate in these areas to feed on the abundant prey, forming temporary groups without overt aggression. Unlike during breeding, there is no territoriality in these non-reproductive contexts, allowing males and females to tolerate the presence of conspecifics of similar size. Several frogs may also share the same tree if food resources are plentiful, indicating resource-driven social tolerance.^[33]^[57]^[7] During hibernation, gray treefrogs seek sheltered microhabitats under leaf litter, logs, or bark to overwinter, where the insulating cover provides thermal and moisture benefits. They are primarily solitary during this period.^[7] Evidence for kin recognition in gray treefrogs is limited, with no robust studies demonstrating discrimination among adults or tadpoles. Tadpole schooling behavior has been observed in some anuran species to mitigate cannibalism risks among siblings, but specific data for gray treefrog larvae are scarce, and opportunistic cannibalism occurs regardless of relatedness.^[58] Communication among gray treefrogs extends beyond acoustic signals to include tactile cues, particularly during mating. Females initiate amplexus by approaching a calling male and touching him with their forelimbs, prompting the male to grasp her in response. This physical contact ensures proper positioning for egg fertilization.^[1]^[7]

Interspecific interactions

The gray treefrog (Hyla versicolor) engages in interspecific competition primarily with its close relative, Cope's gray treefrog (H. chrysoscelis), for calling sites during breeding choruses in sympatric regions. In areas where both species co-occur, such as parts of Missouri, H. versicolor males preferentially select higher and more exposed perches, while H. chrysoscelis males favor lower, more sheltered locations, leading to spatial segregation that minimizes direct overlap.^[59] This partitioning is complemented by acoustic differences, as H. versicolor produces calls with a slower pulse rate (approximately 21.5 pulses per second at 20°C) compared to the faster calls of H. chrysoscelis, facilitating species recognition and reducing interference in noisy choruses.^[60] Such mechanisms likely evolved to mitigate competitive interactions for mates and resources in shared wetland habitats. As part of trophic interactions, H. versicolor serves as prey for various larger herpetofauna and other vertebrates. Bullfrogs (Lithobates catesbeianus), green frogs (L. clamitans), and fishing spiders (Dolomedes triton) commonly consume juvenile and adult gray treefrogs, while eggs and tadpoles are vulnerable to predaceous aquatic insects, salamander larvae, and fish in breeding ponds.^[33] Conversely, H. versicolor acts as a host to several parasites, notably trematode flatworms such as Ribeiroia ondatrae and Alaria species, which infect tadpoles and can cause malformations or increased mortality, with prevalence influenced by environmental factors like agricultural proximity.^[61]^[2] These parasitic interactions highlight the species' role in wetland parasite transmission dynamics, where infection rates are lower in H. versicolor compared to some sympatric anurans due to potential immune advantages.^[2] Hybridization between H. versicolor and H. chrysoscelis occurs occasionally in sympatric zones, producing triploid offspring that are viable but exhibit greatly reduced fertility.^[62] Experimental and field observations indicate that while mixed choruses form, mate discrimination based on call differences limits crossbreeding, though occasional hybrids contribute to gene flow from the diploid H. chrysoscelis into the tetraploid H. versicolor genome. Recent genomic analyses (as of 2022) reveal a complex history of hybridization and whole-genome duplication, with significant gene flow shaping distinct polyploid lineages from ancestral H. chrysoscelis-like populations.^[63]^[64] In commensal relationships, H. versicolor benefits from anthropogenic light sources, often perching near porch lights or building exteriors to exploit concentrations of insects drawn to illumination, enhancing foraging efficiency without apparent cost to humans.^[33] Similarly, the species utilizes beaver (Castor canadensis)-created ponds as breeding sites, where impounded wetlands provide fishless, semi-permanent pools ideal for larval development, indirectly facilitated by beaver engineering.^[65] Within ecological communities, H. versicolor functions as an indicator of wetland health, owing to its biphasic life cycle and permeable skin, which render it sensitive to water quality degradation, habitat fragmentation, and pollutants.^[66] Presence and reproductive success in ponds signal intact ecosystem connectivity between aquatic and terrestrial habitats, with declines often reflecting broader environmental stressors.^[66]

Conservation status

Population trends

The gray treefrog (Dryophytes versicolor) is classified as Least Concern on the IUCN Red List, with the 2015 assessment (published 2017) noting its broad distribution across eastern North America as a buffer against potential declines, and populations remaining stable as of recent evaluations.^[67]^[2] This status reflects the species' abundance in suitable forested and wetland habitats, where it maintains viable populations without evidence of widespread reduction.^[67] Demographics show an approximately balanced sex ratio, with studies reporting roughly 0.73 females per male in surveyed breeding aggregations, and longevity up to 7 years in captivity (wild lifespan unknown).^[68]^[69] Monitoring efforts, including call surveys through the North American Amphibian Monitoring Program (NAAMP), indicate no global population decline. Regionally, populations remain stable in the northeast United States and Canada, while some local decreases have been noted in urbanizing areas of the southern range, potentially linked to habitat fragmentation though not indicative of broader trends.^[70]

Threats and conservation measures

The gray treefrog (Dryophytes versicolor) faces significant threats from habitat loss primarily driven by urbanization and agricultural expansion, which fragment essential wetlands and upland forests used for breeding and foraging. These activities reduce the availability of ephemeral ponds critical for larval development and disrupt migration corridors, leading to isolated populations with decreased genetic diversity.^[1]^[71] Road mortality exacerbates this fragmentation, as adult treefrogs migrating to breeding sites at night are frequently killed by vehicle traffic, with studies showing higher mortality rates near wetlands during peak breeding seasons in spring and summer.^[72]^[73] Emerging threats include sporadic infections from the chytrid fungus Batrachochytrium dendrobatidis (Bd), which causes chytridiomycosis and can increase larval susceptibility to environmental stressors, though prevalence in D. versicolor remains low compared to other amphibians. Pesticides, particularly herbicides like glyphosate (in Roundup) and insecticides like carbaryl, pose lethal risks to tadpoles even at sublethal concentrations found in agricultural runoff, impairing development, growth, and immune function while interacting synergistically with Bd to heighten mortality.^[74]^[75]^[43] Climate change further compounds these pressures by altering breeding phenology, with warmer temperatures advancing chorus initiation and potentially desynchronizing mating calls from optimal conditions, as observed in long-term monitoring across North America. Prolonged droughts reduce pond hydroperiods, causing premature drying that accelerates larval development but lowers survival rates and metamorphic quality in D. versicolor.^[76] Conservation measures focus on mitigating these threats through wetland restoration projects, often supported by easements that protect breeding habitats from development, as implemented in post-mining landscapes in the eastern United States. Establishing vegetated buffer zones around wetlands and forests during forestry operations helps maintain connectivity and reduce edge effects, with guidelines recommending widths of at least 60 meters to support amphibian movements.^[77]^[78]^[2] Ongoing research emphasizes long-term monitoring protocols to detect cryptic population declines, using call surveys and genetic sampling to track Bd prevalence and habitat quality across urban-rural gradients. Recent 2020s studies highlight the need for investigating urban adaptation, such as tolerance to pollutants in stormwater ponds, to inform targeted interventions that enhance resilience in fragmented landscapes.^[79]^[80]^[81]

References

[1]
Gray tree frog - National Zoo
The gray tree frog's scientific name is Hyla versicolor, which comes from the Latin for "variable color." It is named for its ability to alter its skin color ...
[2]
Hyla versicolor - AmphibiaWeb
However, habitat preservation is still important. Hyla versicolor requires terrestrial habitat adjacent to breeding sites as well as the breeding wetlands, with ...
[3]
Gray Treefrog - North Carolina - Davidson College
Gray treefrogs have rough skin, a whitish spot under each eye, and orange spots on hind legs. They can change color and breed in water. Cope's have a harsh ...
[4]
The Complex History of Genome Duplication and Hybridization in ...
The North American gray treefrog complex, consisting of the diploid Hyla chrysoscelis and the tetraploid H. versicolor, has long been used as a model system.<|control11|><|separator|>
[5]
Dryophytes versicolor - NatureServe Explorer
Gray Treefrog - Natureserve Global Rank: G5: This species has a large range in the eastern United States and southeastern Canada.
[6]
Dryophytes versicolor (Gray Treefrog) | BioLib.cz
Gray Treefrog Dryophytes versicolor (LeConte, 1825) ; kingdom Animalia - animals ; phylum Chordata - chordates ; class Amphibia - amphibians ; family Hylidae - tree ...<|separator|>
[7]
Hyla versicolor (Gray Treefrog) - Animal Diversity Web
Conservation Status. Hyla versicolor is not currently classified as endangered or of special concern. However, habitat destruction and human pollutants are ...
[8]
Gray Treefrog - Virginia Herpetological Society
Cope's Gray Treefrogs (Dryophytes chrysoscelis) and Gray Treefrogs (Dryophytes versicolor) are members of a cryptic, diploid-tetraploid species complex. This ...
[9]
Dryophytes versicolor - The Center for North American Herpetology
(2023, Molecular Ecology 32: 4863–4879) provided an updated view of genome duplication and hybridization in this species complex based on genome-wide nDNA data ...
[10]
Amphibians of North Carolina
Holloway et al. (2006) proposed that H. versicolor arose via several speciation events from H. chrysoscelis-like diploid ancestors and two other now extinct ...<|separator|>
[11]
The complete mitochondrial genome of Dryophytes versicolor
In this study, the mitochondrial genome of D. versicolor was sequenced to analyze the phylogenetic relationships among Hylidae and investigate mitochondrial ...Missing: etymology chromosome
[12]
Gray Treefrog Complex - INHS Herpetology Collection
Description: Medium-sized (up to 6 cm SVL) brown or greenish brown frog with a black star-shaped or irregular X-shaped blotch on back.
[13]
Cope's Gray Treefrog (Hyla chrysoscelis) and ... - Carleton College
Jul 26, 2022 · The only way to tell the two species apart is by call (find and listen to their different calls here). Their skin secretions may be irritating ...
[14]
https://www.carleton.edu/arboretum/about/species/fauna/reptiles/gray-treefrog/
[15]
[PDF] Advertisement Call and Distribution of the Treefrogs Hyla ...
ABSTRACT The gray treefrog complex consists of two sibling species that are indistinguishable morphologically, the diploid Hyla chrysoscelis and the tetraploid ...
[16]
Gray Treefrogs (Hyla chrysoscelis/versicolor) - Indiana Herp Atlas
These two frogs are superficially morphologically indistinguishable, but the Eastern Gray Treefrog (Hyla versicolor) is tetraploid (i.e., has four sets of ...Missing: acoustic | Show results with:acoustic
[17]
Cope's Gray Treefrog (Hyla chrysoscelis) - SREL herpetology
Cope's Gray Treefrog is a large treefrog with variable gray to green color, found in the Southeast, and is nocturnal, feeding on small invertebrates.Missing: biology | Show results with:biology
[18]
Gray Treefrog - PA HERP IDENTIFICATION
Scientific Name: Hyla versicolor. Size: 1.6-2 inches (4-5 cm) in length ... The dorsal skin is covered with small warts. The ventral skin is granular ...
[19]
[PDF] CHAPTER 14-1 AMPHIBIANS: ANURAN ADAPTATIONS
Jul 30, 2010 · roughened skin, large toe suction pads, and fringes on the legs, as well as its ability to flatten its body, suggest that it is an arboreal ...Missing: dimorphism | Show results with:dimorphism
[20]
The effect of stress and stress hormones on dynamic colour-change ...
Chromatophores are made up of three cell types that contain different pigments, melanophores (contain melanin), iridophores (light reflecting structures) and ...
[21]
Gray Treefrog - Hyla versicolor - NatureWorks - New Hampshire PBS
Class: Amphibia ; Order: Anura ; Family: Hylidae ; Genus: Hyla ; Characteristics. Gray Tree Frog The gray treefrog is about two inches in length. It is green, gray, ...
[22]
Testing multiple hypotheses on the colour change of treefrogs in ...
Mar 14, 2023 · Evidence to date suggests that thermoregulation and camouflage are the main pressures that influence frogs' adaptive colour change responses.
[23]
Physiological Color Change in the Green Treefrog - jstor
Thus, the control mechanism underlying color change responses to background appearance and to temperature may be related. ... the treefrog, Hyla versicolor Wied.
[24]
Factors Controlling Color Change in the Tree Frog, Hyla versicolor ...
Hyla versicolor responds to light by melanophore expansion, and to darkness by contraction. The completenes of contraction depends upon duration of the ...
[25]
Apparent Continuing Expansion in the Range of the Gray Treefrog ...
Recent observations of the Gray Treefrog, Hyla versicolor, in the Maritimes suggest that this species may be expanding its range and abundance, perhaps partly ...
[26]
Great Smoky Mountains - NPS Natural History Handbook
On the Newfound Gap road, Tennessee side, between 3,500 and 5,000 feet elevation ... The snoring drone of the gray treefrog is one of the late-spring and summer ...
[27]
Hyla chrysoscelis (Cope's Gray Treefrog) - Animal Diversity Web
The male's advertisement call is the main trait to distinguish the eastern gray treefrog ( H. versicolor ) from Cope's gray treefrog ( H. chrysoscelis ). In ...
[28]
Hyla chrysoscelis – H. versicolor Gray Treefrog complex
Years of collection range from 1890 to present. The range extents and areas of sympatry in this species complex are poorly resolved in Arkansas, with H.Missing: km2 | Show results with:km2
[29]
Seasonal Terrestrial Microhabitat Use by Gray Treefrogs (Hyla ...
Sep 1, 2008 · In general, daily activity appeared to be limited to several adjacent trees, and overwintering sites were in close proximity to arboreal ...
[30]
DNR: Fish & Wildlife: Cope's Gray Treefrog and Eastern ... - IN.gov
Habitat. Highly arboreal, found in trees or shrubs in woodlands and swamp forests, adapts well to farmlands and edges of cities. Breeding Habitat.Missing: preferences | Show results with:preferences
[31]
https://www.in.gov/dnr/fish-and-wildlife/wildlife-resources/animals/copes-gray-treefrog-and-eastern-gray-treefrog/
[32]
Gray Treefrog and Cope's Gray Treefrog | Missouri Department of ...
Gray treefrogs overwinter belowground. Like some other frogs, they produce a substance in their blood that functions as antifreeze. See Habitat and Conservation ...<|separator|>
[33]
[PDF] Wetland Buffer Zones and Beyond - Shutesbury.org
The Grey Treefrog is generally found in moist areas during the summer and is ... Habitat Loss and Fragmentation for Wetland Amphibian Assemblages Wetlands,.Missing: Gray | Show results with:Gray
[34]
[PDF] AGRICULTURAL PONDS SUPPORT AMPHIBIAN POPULATIONS
Gray treefrog reproductive success was higher ... Because of the high risk of predation by fish, most amphibians require fishless habitats to breed and survive ( ...
[35]
Eastern Grey Treefrog - Mountain Lake Biological Station
Jul 10, 2014 · H. versicolor emerges from hibernation in the early spring, but must build up its energy reserves before beginning to call.Missing: facts | Show results with:facts
[36]
The cryoprotectant system of Cope's gray treefrog, Dryophytes ... - NIH
Cope's gray treefrog (Dryophytes chrysoscelis) is one of few freeze-tolerant frogs that mobilize glycerol as a cryoprotectant, yet cold and freezing-induced ...
[37]
[PDF] Southern Gray Treefrog - NJ.gov
High humidity and temperatures above 17°C initiate breed- ing activity. Voice: The Northern Gray Treefrog has a slow trill while that of the Southern Gray ...
[38]
https://www.nj.gov/dep/fgw/ensp/pdf/species/so_gray_treefrog.pdf
[39]
Diet of the Gray Treefrog (Hyla Versicolor) in Relation to Foraging ...
Aug 6, 2025 · Females captured farther from breeding ponds tend to have greater stomach content mass than do those captured near breeding ponds (Mahan and ...
[40]
Gray Treefrog | Oklahoma Department of Wildlife Conservation
Gray treefrogs (Hyla versicolor) and Cope's gray treefrogs (Hyla chrysoscelis) are two of the most common treefrogs in the eastern half of the United States.Missing: Rana synonym
[41]
Hidden black and yellow thigh color acts as an aposematic signal in ...
Sep 5, 2023 · Our findings support the hypothesis that hidden color patches act as aposematic signals in Gray Treefrogs and suggest that more species benefit ...
[42]
Predator-induced stress makes the pesticide carbaryl more ... - PNAS
We have shown that very low concentrations of just one pesticide can cause high rates of mortality in gray treefrogs.
[43]
Costs and Benefits of a Predator-Induced Polyphenism in the Gray ...
-The phenotypes of gray treefrog (Hyla chrysoscelis) tadpoles vary depending on whether predators are ... ing that predation would reduce tadpole survival by 70%.
[44]
[PDF] Effect of body size, age and timing of breeding on clutch and egg ...
Dec 30, 2021 · We collected clutch size (fecundity) and egg size data of female Eastern Gray Treefrogs, Hyla versicolor, and examined whether observed ...Missing: hormonal | Show results with:hormonal
[45]
Conspecific acoustic signals influence ovarian development and ...
... acoustic signals influence ovarian development and hormonal levels in female gray treefrogs (Hyla versicolor). Conspecific acoustic signals can act as cues ...
[46]
Hormonal modulation of phonotaxis and advertisement-call ...
We found that female gray treefrogs (Hyla versicolor) treated with progesterone and prostaglandin exhibited phonotaxis to synthetic male advertisement signals ...
[47]
https://pubmed.ncbi.nlm.nih.gov/18926827/
[48]
Life History of the Gray Treefrog (Hyla chrysoscelis) in - jstor
before sunrise. Most males called from perches above ground. Average perch height of calling males was 1.51 m (SD = ...
[49]
None
### Mating Behaviors in Gray Treefrog (Hyla versicolor)
[50]
None
### Summary of Male-Male Competition in Gray Treefrogs (Hyla versicolor)
[51]
The role of body size on the outcome, escalation and duration of ...
We found a weak but statistically significant relationship between body size and success in aggressive interactions in H. versicolor.Missing: wrestling | Show results with:wrestling
[52]
[PDF] Advertisement call duration indicates good genes for offspring ...
gray tree frogs (Hyla versicolor) prefer male advertise- ment calls of long call duration, which can be indicators of enhanced offspring growth performance ...
[53]
Amphibian Egg Mass Identification - Field Herp Forum
Jun 10, 2010 · The versicolor/chrysoscelis eggs I have found were freshly laid, but floating on the surface. Maybe they sink after they begin developing.
[54]
[PDF] A Field Guide to Amphibian Larvae and Eggs of Minnesota ...
Gray and Cope's Gray Treefrog (Hyla versicolor/H. chrysoscelis) ... Gray Treefrogs and Cope's Gray Treefrogs cannot be distinguished based on morphology;.
[55]
(PDF) Comparative feeding kinematics of temperate pond-dwelling ...
Aug 6, 2025 · ... dental formula, with 2 anterior and 3 posterior. (hereafter, ''2/3 ... The larvae of Hyla chrysoscelis (Cope's gray treefrog; hereafter ...
[56]
Gray Treefrog | Reptiles & Amphibians in Ontario
The gray treefrog (Hyla versicolor), as its Latin name suggests, has the chameleon-like ability to change its colour.
[57]
[PDF] Phenotypic plasticity in amphibians - School of Natural Resources
How would kin relationships affect cannibalism? If reared with siblings ... Gray treefrogs (Hyla versicolor). Wood frogs (Rana sylvatica). Recently ...
[58]
calling sites used by male gray treefrogs, hyla versicolor and ... - jstor
1992 by The Herpetologists' League, Inc. CALLING SITES USED BY MALE GRAY TREEFROGS,. HYLA VERSICOLOR AND HYLA CHRYSOSCELIS, IN. SYMPATRY AND ALLOPATRY IN ...
[59]
Species Recognition Is Constrained by Chorus Noise, but Not ...
Jul 30, 2020 · We assayed female discrimination based on pulse rate, a signal of species identity, in quiet and at three levels of ambient noise designed to ...
[60]
Environmental factors influencing trematode prevalence in grey tree ...
The emergence or increased prevalence of various parasites may be linked to alterations in host-parasite interactions caused by environmental changes.
[61]
Hybridization in the Diploid-Tetraploid Treefrogs Hyla chrysoscelis ...
versicolor. NATURAL hybridization among North. American treefrogs has been frequently reported; most hybrids were males that were.
[62]
Revisiting the evolution of the North American tetraploid treefrog ...
Aug 13, 2020 · Hyla chrysoscelis and H. versicolor are common treefrogs in eastern North America and are a cryptic diploid-tetraploid species pair.
[63]
Gray Treefrog: Reptiles & Amphibians: Species Information: Wildlife
On this page: Distinguishing Characteristics; Status and Distribution in Maine; Habitat; Diet; Seasonal Changes; Natural History Notes; Share Your Sighting.Missing: predators | Show results with:predators
[64]
[PDF] 12 Using Amphibians in Bioassessments of Wetlands
Use of amphibians as indicators of the health of individual wetlands may be affected by their population structure. Wetland ecosys- tems need to be assessed ...
[65]
https://www.maine.gov/ifw/fish-wildlife/wildlife/species-information/reptiles-amphibians/gray-treefrog.html
[66]
Assessing Summer Pond and Lake Inlet Use by Gray Treefrogs ...
Aug 2, 2021 · We identified the sex of 138 individuals (58 females and 80 males) for an overall sex ratio of 0.73 females per male.
[67]
Gray Treefrog | The Canadian Encyclopedia
Nov 24, 2023 · The gray treefrog (Dryophytes versicolor, synonym Hyla versicolor) is a small to medium-sized treefrog native to Central and Northeastern ...Missing: original author
[68]
[PDF] Monitoring long-term trends in Wisconsin frog and toad populations
The North American Amphibian Monitor- ing Program (NAAMP) was initiated in ... gray treefrog and American toad populations appear to be stable or ...
[69]
southeast regional and state trends in anuran occupancy from ...
Jan 11, 2019 · We found weak evidence for a general regional pattern of decline in calling anurans within breeding habitats along roads in the southeastern USA ...
[70]
Cope's Gray Treefrog | Nebraskaland Magazine
Apr 3, 2024 · These arboreal amphibians are predominantly nocturnal, seeking refuge in shaded areas or tree cavities during the day and emerging at night to ...
[71]
Influence of the COVID‐19 pandemic on amphibian road mortality
Sep 29, 2021 · Some species, such as the gray tree frog (Hyla versicolor) may feature migratory behavior but are much less temporally concentrated ...
[72]
The relative effects of road traffic and forest cover on anuran ...
Three of six common species showed stronger associations with traffic density than with forest cover –Bufo americanus, Rana pipiens, and Hyla versicolor ...
[73]
Amphibian Diseases | U.S. Geological Survey - USGS.gov
A Gray treefrog (Hyla versicolor); takes advantage of its gray color to camoflauge ... Chytrid fungus (Batrachochytrium dendrobatidis, Bd) was identified ...
[74]
Microplastics increase susceptibility of amphibian larvae to ... - Nature
Nov 17, 2021 · In tandem, the amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) causing the disease chytridiomycosis ... (Hyla versicolor) larvae.
[75]
Effects of Disease and Pond Drying on Gray Tree Frog Growth ...
Aug 9, 2025 · In a study comparing parasitic trematode infection of the gray tree frog (Hyla versicolor), a controlled gradual decline in pond water ...
[76]
Wetland Creation and Reforestation of Legacy Surface Mines in the ...
Apr 23, 2024 · ... Hyla versicolor) and Cope's Gray treefrog (H. chrysoscelis)) are ... adaptation strategies for wildlife management and biodiversity conservation.
[77]
[PDF] NJ Wildlife Action Plan: 01/23/08
Wetland Buffer Guidelines for Species of Conservation Concern in New Jersey prep). Stabilize wetland buffers and streambanks by encouraging plantings of.
[78]
[PDF] INVENTORy AND MONITORING
This inventory and monitoring initiative has been established through partnerships among Partners in Amphibian and Reptile Conservation ... Hyla versicolor, H.
[79]
Amphibian monitoring in hardwood forests - Oxford Academic
Aug 5, 2019 · Hyla versicolor/chrysoscelis complex. Gray treefrog complex. Xb. X. X. X ... for amphibians into more general restoration monitoring plans, when ...
[80]
Changes in reptile and amphibian communities across urbanization ...
We combined subspecies into single species, and we combined the two Gray Treefrog species (Hyla versicolor ... This suggests a strategy for urban conservation ...