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Intestinal permeability

Intestinal permeability is the functional property of the intestinal epithelium that regulates the selective passage of ions, water, nutrients, and other molecules from the gut lumen into the bloodstream, while acting as a barrier to prevent harmful substances such as pathogens, toxins, and undigested food particles from entering systemic circulation.^[1] This process occurs across a vast surface area of approximately 32 square meters, primarily in the small intestine, requiring a substantial portion of the body's energy expenditure to maintain barrier integrity and homeostasis.^[2] Under normal conditions, it facilitates essential physiological functions like nutrient absorption (e.g., glucose and electrolytes via transcellular routes) and immune surveillance through the gut-associated lymphoid tissue, supported by a mucus layer and secretory immunoglobulins such as IgA.^[3] The intestinal barrier's permeability is primarily controlled by tight junctions (TJs), protein complexes including claudins, occludin, and zonula occludens-1 (ZO-1) that seal the paracellular space between epithelial cells, alongside transcellular mechanisms involving transporters and endocytosis for larger or specific molecules.^[4] Regulation involves multiple factors, including the gut microbiota, which modulates TJ expression and barrier function through short-chain fatty acids and probiotics like Escherichia coli Nissle 1917; dietary components such as high-fat diets or gluten that can disrupt TJs; and humoral modulators like zonulin, a protein that reversibly opens TJs to increase permeability in response to physiological stimuli.^[1] Stress, inflammation, and environmental factors (e.g., microplastics or nanoparticles) further influence this dynamic equilibrium, with vitamins A and D enhancing barrier integrity by promoting TJ assembly.^[4] Pathological increases in intestinal permeability, often termed "leaky gut," occur when TJ integrity is compromised, allowing translocation of luminal contents that trigger local and systemic inflammation via immune activation and bacterial products like lipopolysaccharides.^[3] This dysfunction is implicated in a range of gastrointestinal disorders, including inflammatory bowel disease (e.g., Crohn's disease and ulcerative colitis, where elevated permeability precedes clinical relapse), celiac disease (driven by gluten-induced zonulin release), and irritable bowel syndrome.^[1] Beyond the gut, it contributes to systemic conditions such as type 2 diabetes, obesity, rheumatoid arthritis, non-alcoholic fatty liver disease, and even neurological disorders like Parkinson's disease, through the gut-liver axis or microbiota-brain interactions that amplify chronic low-grade inflammation; as of 2025, elevated permeability biomarkers are also prospectively linked to incident cardiovascular disease (e.g., myocardial infarction and stroke) and depression.^[4]^[5]^[6] Emerging therapies target permeability restoration, including zonulin antagonists, probiotics, and dietary interventions like glutamine supplementation, with recent 2024 studies highlighting exercise's role in improving microbiota composition and barrier function.^[4]

Overview and Physiology

Definition and Importance

Intestinal permeability refers to the capacity of the intestinal epithelium to selectively permit the passage of essential substances, such as nutrients, ions, and water, while forming a robust barrier against the translocation of harmful luminal contents, including pathogens and toxins.^[7] This regulated process occurs primarily through transcellular and paracellular routes, ensuring efficient absorption without compromising barrier integrity.^[8] The importance of intestinal permeability lies in its central role in physiological homeostasis, where it balances nutrient uptake with protection against microbial invasion, thereby preventing systemic immune activation from inappropriate exposure to gut contents.^[9] Disruptions in this barrier function, leading to increased permeability, have been implicated in the initiation and progression of chronic inflammatory conditions by allowing the leakage of bacterial products like lipopolysaccharides into the bloodstream.^[10] Historically, investigations into intestinal absorption began in the 19th century through physiological studies on nutrient transport across the gut wall, laying foundational insights into barrier dynamics.^[11] Modern understanding advanced significantly in the mid-20th century with the electron microscopic discovery of tight junctions in 1963, followed by 1980s research elucidating their role in modulating permeability.^[12] In healthy individuals, baseline intestinal permeability maintains low paracellular flux, restricting the passage of macromolecules to negligible levels and thereby minimizing endotoxemia risk; in contrast, hyperpermeability states elevate this flux, facilitating harmful substance translocation and contributing to immune dysregulation.^[7]

Structure of the Intestinal Barrier

The intestinal barrier consists of multiple layered components that collectively prevent the passage of harmful substances while allowing selective absorption. The outermost layer is the mucus, produced by goblet cells, which forms a protective coating over the epithelial surface. Beneath this lies a single monolayer of epithelial cells, including enterocytes for absorption and Paneth cells for antimicrobial defense, sealed by tight junctions to regulate paracellular permeability. The underlying lamina propria contains immune cells that contribute to barrier surveillance and response.^[13] The mucus layer is a gel-like structure primarily composed of glycoproteins, such as MUC2 mucin secreted by goblet cells, which expands upon release to create a stratified barrier. In the colon, it features an inner adherent layer that remains largely sterile and an outer loose layer harboring commensal microbes, while the small intestine has a single, thinner, non-stratified mucus layer. This hierarchical organization, with trefoil factor family peptides like TFF3 and FCGBP enhancing stability through disulfide bonds, physically separates luminal contents from the epithelium.^[14]^[15] The epithelial monolayer is a contiguous sheet of polarized cells, dominated by enterocytes that form the absorptive surface, alongside goblet cells for mucus production and Paneth cells located in crypts that secrete antimicrobial peptides. These cells maintain structural integrity through apical microvilli for increased surface area and basolateral connections to the basement membrane. The lamina propria, a connective tissue layer beneath the epithelium, houses immune cells such as dendritic cells, macrophages, and lymphocytes, which monitor and reinforce the barrier without direct luminal exposure.^[15]^[13] Tight junctions form intricate protein complexes at the apical-lateral borders of epithelial cells, sealing paracellular spaces to restrict ion and solute diffusion. Core transmembrane proteins include claudins (a family of over 20 members forming selective strands), occludin (a tetraspan protein stabilizing the junction), and junctional adhesion molecules (JAMs) that mediate cell-cell adhesion. Scaffolding proteins like zonula occludens-1 (ZO-1) link these to the actin cytoskeleton, while adherens junctions involving E-cadherin provide additional mechanical stability.^[12]^[15] Transcellular permeability is facilitated by elements within epithelial cells, including endocytic vesicles that enable vesicular transport of macromolecules via transcytosis and membrane transporters embedded in the plasma membrane. For instance, PEPT1, a proton-coupled oligopeptide transporter on the apical membrane of enterocytes, structurally consists of 12 transmembrane helices and facilitates the uptake of di- and tripeptides into the cell interior. These components allow controlled passage through the cell without compromising the overall barrier.^[16]^[17] Regional variations in barrier structure adapt to functional demands, with the small intestine featuring a looser mucus layer and more permeable epithelium optimized for nutrient absorption, whereas the colon exhibits a thicker, stratified mucus and tighter junctions (e.g., higher expression of sealing claudins) to handle fermentation byproducts and denser microbiota. These differences arise from variations in cell composition, such as greater goblet cell density in the colon and Paneth cell prominence in the small intestine.^[15]^[13] The intestinal barrier undergoes significant maturation during infancy, driven by microbial colonization that begins at birth and shapes structural development. Early microbiota establishment promotes epithelial proliferation, tight junction assembly, and mucin production, with commensal bacteria inducing antimicrobial peptide secretion from Paneth cells and enhancing goblet cell function. Without timely colonization, such as in germ-free models, the barrier remains immature with increased permeability, but restoration via microbiota transfer can normalize these features within critical postnatal windows.^[18]

Mechanisms of Permeability

Intestinal permeability is primarily governed by two distinct pathways: the paracellular route, which facilitates the diffusion of small ions and hydrophilic molecules between adjacent epithelial cells via tight junctions, and the transcellular route, which enables the transport of solutes and larger entities across the interior of the cells.^[19] The paracellular pathway is a passive process regulated by the dynamic assembly and disassembly of tight junction strands, allowing selective passage based on molecular size and charge while maintaining barrier integrity under normal physiological conditions.^[20] This route is crucial for the absorption of ions like sodium and small nutrients, with permeability tightly controlled to prevent uncontrolled leakage.^[21] In contrast, the transcellular pathway involves energy-dependent mechanisms that exploit the apical-basolateral polarity of intestinal epithelial cells. Active transport carriers, such as the sodium-glucose linked transporter 1 (SGLT1), mediate the uptake of glucose from the luminal side coupled with sodium influx, followed by facilitated diffusion across the basolateral membrane.^[22] For larger molecules, including proteins and lipids, endocytosis allows internalization at the apical membrane and subsequent transcytosis or exocytosis at the basolateral side, ensuring directed vectorial transport.^[23] This pathway supports the absorption of essential macronutrients and maintains cellular homeostasis by coupling transport to metabolic energy.^[24] The intestinal barrier exhibits selective permeability, distinguishing between paracellular and transcellular routes based on molecular properties. Paracellular transport is generally limited to hydrophilic molecules smaller than approximately 500 Da, with charge selectivity influenced by tight junction proteins that form cation- or anion-preferring pores.^[25] Transcellular mechanisms, however, are often active and energy-dependent for polar solutes, while passive diffusion predominates for lipophilic compounds that partition into the cell membrane.^[19] This selectivity ensures efficient nutrient uptake without compromising barrier function against pathogens or toxins. Homeostatic balance in intestinal permeability is maintained through feedback mechanisms involving cytokine signaling, which fine-tunes epithelial flux in response to luminal contents and immune cues. For instance, the anti-inflammatory cytokine interleukin-10 (IL-10) reinforces barrier integrity by suppressing pro-inflammatory signals that could widen junctions, thereby adjusting permeability to preserve physiological equilibrium.^[19] Such regulatory loops prevent excessive paracellular leakage while supporting adaptive transport needs. Quantitative assessment of permeability often employs the apparent permeability coefficient (P_app) in in vitro models to characterize flux across epithelial monolayers. This metric is calculated as P_{\text{app}} = \frac{dQ/dt}{A \cdot \Delta C}, where dQ/dt represents the steady-state flux rate, A is the surface area, and \Delta C is the concentration difference across the barrier.^[26] This formula provides a standardized measure of transport efficiency, aiding in the distinction between passive diffusion and carrier-mediated processes under controlled conditions.

Regulation and Measurement

Physiological and Pathological Modulators

Intestinal permeability is tightly regulated by various physiological factors that maintain barrier integrity under normal conditions. Microbiota-derived short-chain fatty acids (SCFAs), such as butyrate, enhance tight junction assembly and function in intestinal epithelial cells, thereby strengthening the paracellular barrier and reducing permeability.^[27] These metabolites, produced through bacterial fermentation of dietary fibers, promote the expression of tight junction proteins like zonula occludens-1 and occludin, contributing to overall gut homeostasis.^[28] Similarly, the hormone glucagon-like peptide-2 (GLP-2), secreted by enteroendocrine L cells in response to nutrient intake, promotes intestinal barrier integrity by increasing transepithelial electrical resistance and enhancing both paracellular and transcellular transport pathways.^[29] GLP-2 achieves this through upregulation of tight junction proteins and cytoskeletal elements, preventing excessive leakage while supporting nutrient absorption.^[30] Recent studies as of 2024 have further highlighted the role of enteroendocrine cells in regulating intestinal barrier permeability through hormone secretion and direct epithelial interactions.^[31] Nutritional components also play a critical role in modulating permeability, with certain nutrients supporting barrier maintenance and others potentially disrupting it in susceptible individuals. Glutamine serves as the primary metabolic fuel for enterocytes, the absorptive cells of the intestinal epithelium, where it supports cellular proliferation and repair, thereby preserving tight junction integrity and reducing permeability.^[32] Supplementation with glutamine has been shown to decrease intestinal permeability in stressed or inflamed states by maintaining enterocyte energy supply and mitigating epithelial damage.^[33] In contrast, gluten-derived peptides like gliadin can trigger zonulin release from intestinal epithelial cells in genetically predisposed individuals, leading to reversible opening of tight junctions and increased paracellular permeability.^[34] This zonulin-mediated effect occurs independently of overt autoimmunity but is amplified in conditions like celiac disease, where gliadin binding to chemokine receptor CXCR3 initiates the signaling cascade. Pathological conditions often involve modulators that compromise the intestinal barrier, primarily through inflammatory and oxidative mechanisms. Proinflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ), disrupt tight junction structure and function during intestinal inflammation by downregulating key proteins like claudins and occludins, resulting in elevated paracellular permeability.^[35] These cytokines act synergistically to alter the actin cytoskeleton and increase myosin light chain phosphorylation, further impairing barrier selectivity.^[36] Nonsteroidal anti-inflammatory drugs (NSAIDs) exacerbate this by inducing oxidative stress in the intestinal mucosa, which damages enterocytes and elevates permeability through lipid peroxidation and mitochondrial dysfunction.^[37] Chronic alcohol exposure similarly promotes hyperpermeability via oxidative stress, where ethanol metabolism generates reactive oxygen species that degrade tight junction complexes and facilitate bacterial translocation.^[38] External stressors, including psychological and infectious challenges, can acutely alter permeability through neuroendocrine and microbial pathways. Psychological stress activates the hypothalamic-pituitary-adrenal axis, releasing corticotropin-releasing factor (CRF), which increases intestinal permeability in a mast cell-dependent manner by promoting cytokine release and protease activity that loosen tight junctions.^[39] This CRF-mediated effect mimics acute stress responses and can be blocked by mast cell stabilizers, highlighting the gut-brain axis's role in barrier regulation.^[40] Bacterial infections, such as those caused by Salmonella species, further increase permeability by upregulating "leaky" tight junction proteins like claudin-2 and disrupting epithelial integrity through effector proteins and toxins.^[41] In this context, enteric pathogens trigger zonulin secretion as part of the host's innate response, which transiently enhances paracellular flux to expel microbes but can lead to prolonged barrier defects if unresolved. Genetic factors underlie certain hereditary defects in intestinal permeability by impairing tight junction formation. Mutations in the CLDN1 gene, which encodes claudin-1—a major component of tight junctions—disrupt epithelial sealing and are linked to hereditary conditions featuring increased paracellular leakage, such as neonatal sclerosing cholangitis associated with ichthyosis. These mutations reduce claudin-1 polymerization in the junctional strand, compromising barrier selectivity and allowing inappropriate passage of luminal contents.^[42] Claudin-1 deficiency thus exemplifies how genetic variations can predispose to baseline permeability alterations, independent of environmental triggers.^[43] As of 2025, emerging research identifies myosin light chain kinase (MLCK) as a key regulator of tight junction permeability, representing a promising therapeutic target for restoring barrier function in inflammatory conditions.^[44]

Assessment Methods

Intestinal permeability is assessed using a variety of in vivo, ex vivo, and biomarker-based techniques to evaluate the integrity of the intestinal barrier. These methods aim to quantify paracellular and transcellular pathways through which solutes and macromolecules may pass, providing insights into barrier dysfunction. In vivo approaches often involve non-invasive probe administration, while ex vivo and imaging techniques offer more direct measurements but may require invasive sampling. Limitations across methods include inter-individual variability in probe absorption, metabolism, and renal clearance, which can confound results.^[45] Probe-based tests represent a cornerstone of in vivo assessment, particularly the lactulose-mannitol (L:M) dual-sugar test, which differentiates paracellular from transcellular permeability. In this procedure, participants ingest a solution containing 5 g lactulose (a disaccharide marker for larger paracellular pores, 342 Da) and 2 g mannitol (a monosaccharide for smaller transcellular pores, 182 Da) after overnight fasting, followed by collection of all urine over 5 hours. Urinary concentrations are measured via high-performance liquid chromatography, and the L:M excretion ratio is calculated; a normal ratio is approximately 0.03 (range 0.003-0.25 in healthy individuals), with elevated ratios (>0.03) indicating increased paracellular leakage, as lactulose recovery exceeds that of mannitol in barrier-compromised states. This test is widely used due to its non-invasiveness and ability to reflect small intestinal permeability, though gastric emptying and renal function can influence outcomes. Other probes, such as polyethylene glycols (PEG 400/1000) or 51Cr-EDTA, provide complementary site-specific data but share similar variability issues.^[45]^[46] Biomarker assays offer indirect, non-invasive evaluation through circulating or fecal indicators of barrier integrity. Serum zonulin, a 47 kDa protein that modulates tight junctions, is commonly measured via enzyme-linked immunosorbent assay (ELISA); levels above 30-34 ng/mL suggest hyperpermeability, correlating with conditions like celiac disease where zonulin upregulation increases intestinal leakiness. However, assay reliability is debated, as some commercial kits may detect unrelated proteins like haptoglobin, leading to calls for standardized validation. Lipopolysaccharide-binding protein (LBP), an acute-phase reactant, assesses microbial translocation and endotoxemia by binding bacterial lipopolysaccharide (LPS); elevated serum LBP levels (>10 μg/mL in some cohorts) indicate barrier breach allowing luminal bacteria-derived products into circulation, though diet and infection can elevate it independently. These biomarkers provide accessible screening but lack specificity for permeability alone.^[47]^[48]^[46] In vivo imaging techniques enable real-time visualization of barrier dynamics. Confocal laser endomicroscopy (CLE), performed during endoscopy, uses fluorescein sodium (100 mg IV) to highlight epithelial gaps and cell shedding; increased fluorescein leakage into the lamina propria (quantified as cell junctions extruded or fluorescein leak score) signifies local permeability defects, as validated in inflammatory bowel disease where it predicts relapse with high sensitivity. In animal models, FITC-dextran gavage (e.g., 4 kDa FITC-dextran at 60 mg/100 g body weight) measures serum fluorescence 1-4 hours post-oral administration via fluorimetry; elevated plasma levels (>0.5 μg/mL) denote small intestinal permeability, offering a translational tool for preclinical studies though limited by species-specific gut transit. These methods provide spatial resolution but require specialized equipment and expertise.^[49]^[50] Ex vivo assessments, such as Ussing chambers, allow precise quantification of ion and solute flux in isolated tissue. Biopsies or mucosal sheets from endoscopy are mounted between chambers filled with oxygenated Ringer's solution at 37°C, enabling measurement of transepithelial electrical resistance (TEER, normal >50 Ω·cm² for human ileum) via Ag/AgCl electrodes and flux of probes like 4 kDa FITC-dextran or 51Cr-EDTA across the tissue (apparent permeability coefficient, Papp <10^{-6} cm/s in intact barriers). Short-circuit current recordings further assess active transport; reduced TEER or increased flux indicates permeability alterations, distinguishing paracellular (e.g., EDTA) from transcellular routes. This gold-standard technique is ideal for mechanistic studies but is invasive and reflects only the sampled region.^[51]^[52] Emerging multi-omics approaches integrate permeability markers with broader profiling for holistic insights. For instance, combining fecal calprotectin (a neutrophil-derived inflammation marker, normal <50 μg/g) with serum zonulin or probe-derived data via metagenomics, metabolomics, and proteomics reveals microbial-host interactions driving barrier dysfunction, as seen in cohorts where elevated calprotectin correlates with dysbiosis-linked permeability. These high-throughput methods, using stool samples, overcome single-marker limitations but face challenges in standardization and interpretation due to probe metabolism variability and confounding factors like microbiota composition.^[53]^[54] As of 2025, advancements include systematic evaluations of in vitro intestinal permeability assays like Caco-2 models and multi-sugar assays for non-invasive whole-gut assessment, enhancing precision in clinical and preclinical research.^[55]^[56]

Clinical and Pathophysiological Implications

Associations with Diseases

Altered intestinal permeability, often termed "leaky gut," has been implicated in the pathogenesis of various gastrointestinal (GI) disorders. In celiac disease, gluten ingestion triggers a rapid spike in zonulin release, a key regulator of tight junctions, leading to increased epithelial permeability that precedes the onset of autoimmunity and villous atrophy.^[57]^[58] This early permeability allows gliadin peptides to translocate across the barrier, initiating immune responses. Similarly, in inflammatory bowel disease (IBD), particularly Crohn's disease, hyperpermeability is observed in a substantial proportion of patients, with tumor necrosis factor-alpha (TNF-α) playing a central role by disrupting tight junction proteins like occludin and claudins, thereby exacerbating mucosal inflammation.^[59]^[60] Antitumor necrosis factor-α therapies have been shown to restore barrier integrity in these cases.^[59] Beyond GI conditions, increased intestinal permeability correlates with metabolic diseases. In type 1 and type 2 diabetes, leaky gut facilitates bacterial translocation and endotoxemia, contributing to insulin resistance and β-cell dysfunction; studies indicate that hyperglycemia itself drives barrier impairment, with associations noted in patients exhibiting poor glycemic control.^[61]^[62] In obesity, particularly among individuals with a body mass index (BMI) greater than 30 kg/m², the lactulose:mannitol (L:M) ratio—a marker of paracellular permeability—is elevated, linking barrier dysfunction to systemic low-grade inflammation and metabolic syndrome components.^[63]^[64] Recent studies from 2023 to 2025 highlight further associations. A 2024 meta-analysis found that 70% of investigations in irritable bowel syndrome with diarrhea (IBS-D) reported elevated intestinal permeability compared to controls, suggesting it as a contributing factor to symptom severity.^[65] In liver cirrhosis, particularly decompensated cases, serum zonulin levels are elevated, indicating heightened permeability that promotes bacterial translocation and complications like spontaneous bacterial peritonitis.^[66]^[67] Mechanistically, antigen translocation due to compromised permeability triggers Th17 cell responses, amplifying autoimmunity; in celiac disease, this occurs pre-symptomatically, with gliadin-induced zonulin release enabling peptide access to lamina propria immune cells.^[68]^[69] Epidemiological data from 2025 studies suggest that hyperpermeability increases in aging populations, potentially driven by age-related declines in tight junction integrity, increasing susceptibility to these diseases.^[70]

Role in Systemic Inflammation and Comorbidities

Intestinal hyperpermeability facilitates the translocation of bacterial lipopolysaccharides (LPS) from the gut lumen into the systemic circulation, a process known as metabolic endotoxemia, which triggers low-grade chronic inflammation. This occurs primarily through the activation of Toll-like receptor 4 (TLR4) on immune cells, leading to the release of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6).^[71] Elevated serum LPS levels, often exceeding 0.5 EU/mL in affected individuals, serve as a biomarker for this endotoxemia and correlate with sustained systemic inflammatory responses that extend beyond the gastrointestinal tract.^[72] Gut dysbiosis exacerbates this by increasing the production of pro-inflammatory microbial components, further compromising barrier integrity and perpetuating the inflammatory cycle.^[73] This systemic inflammation links intestinal hyperpermeability to various comorbidities, notably cardiovascular disease (CVD). A 2024 meta-analysis of clinical studies demonstrated that patients with CVD exhibit significantly elevated markers of intestinal permeability, such as zonulin and LPS-binding protein (LBP), indicating barrier dysfunction as a contributing factor to atherosclerosis and endothelial inflammation.^[74] In depression, circulating zonulin levels positively correlate with symptom severity, as measured by Beck Depression Inventory scores above 20, suggesting that leaky gut contributes to neuroinflammatory processes via the gut-brain axis.^[6] These associations highlight how intestinal barrier impairment drives extra-intestinal pathologies through immune activation and microbial metabolite dissemination. Along the gut-brain axis, hyperpermeability promotes neuroinflammation in conditions like Parkinson's disease by allowing neurotoxic metabolites and inflammatory mediators to reach the central nervous system. Studies from 2024 indicate that increased intestinal permeability in Parkinson's patients correlates with α-synuclein aggregation and dopaminergic neuron loss, exacerbated by microbiota-derived short-chain fatty acids and LPS that amplify microglial activation.^[75] This bidirectional interplay underscores the role of gut barrier dysfunction in neurodegenerative progression. In aging populations, intestinal hyperpermeability contributes to chronic low-grade inflammation, with 2025 cohort data revealing elevated LBP levels in elderly individuals, linking gut leakage to frailty and multimorbidity.^[76] Similarly, in intensive care unit (ICU) patients, dysbiosis initiates a vicious loop where microbial shifts increase permeability, leading to endotoxemia, sepsis, and multi-organ dysfunction; this permeability-dysbiosis cycle is a common feature in critically ill cases.^[77] Regarding cancer, intestinal hyperpermeability may influence tumor progression through inflammation and immune dysregulation. Leaky gut thus facilitates a pro-metastatic microenvironment by enabling the systemic spread of oncogenic signals and inflammatory factors.^[78]

Therapeutic and Modulatory Strategies

Dietary and Lifestyle Interventions

Dietary interventions play a significant role in modulating intestinal permeability by influencing the gut microbiota and epithelial barrier function. High-fiber intake, typically recommended at 25-30 grams per day, promotes the production of short-chain fatty acids (SCFAs) such as butyrate through fermentation by gut bacteria, which in turn enhances tight junction integrity and reduces paracellular permeability.^[79]^[80] The Mediterranean diet, rich in fruits, vegetables, whole grains, and healthy fats, has been shown to improve intestinal barrier function, with recent trials, such as the LIBRE trial, demonstrating improved intestinal barrier function associated with increased plasma n-3 polyunsaturated fatty acids in participants adhering to this pattern.^[81]^[82] Incorporating fermented foods, such as yogurt, kefir, and sauerkraut, supports microbiota restoration and helps maintain epithelial barrier homeostasis by increasing microbial diversity and reducing inflammation-associated permeability.^[83]^[84] Regarding specific supplements, randomized controlled trials (RCTs) on glutamine at 10 grams per day have yielded inconsistent results for improving permeability, with some showing no overall effect despite benefits in select populations.^[85]^[86] In contrast, omega-3 fatty acids, particularly 2 grams of combined eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) daily, have demonstrated benefits in inflammatory bowel disease (IBD), reducing inflammation and supporting barrier repair as evidenced by RCTs.^[87]^[88] Lifestyle modifications further contribute to permeability regulation. Moderate aerobic exercise, such as brisk walking for 150 minutes per week, prevents stress-induced increases in permeability by mitigating inflammatory responses, though high-intensity sessions exceeding 80% of VO2 max can exacerbate leaks due to splanchnic hypoperfusion.^[89]^[90] Stress reduction techniques, including mindfulness-based practices, lower corticotropin-releasing factor (CRF) levels, which otherwise promote mast cell degranulation and barrier disruption.^[91]^[92] Alcohol abstinence rapidly improves permeability markers; for instance, one week of withdrawal in patients with alcohol-related liver disease significantly increases zonulin levels toward normal (p < 0.05) and reduces intestinal fatty acid-binding protein (I-FABP) levels, indicating restored tight junction function.^[93]^[94] In individuals with metabolic dysfunction-associated steatohepatitis (MASH), achieving 5-10% body weight reduction through diet and exercise enhances permeability, as shown in 2024 studies linking weight loss to decreased microbial translocation and improved barrier integrity.^[95]^[96]

Pharmacological and Microbial Therapies

Pharmacological therapies targeting intestinal permeability primarily focus on modulating tight junction proteins and reducing inflammation to restore barrier integrity. Larazotide acetate, a zonulin antagonist, inhibits the disassembly of tight junctions induced by gluten in celiac disease, thereby decreasing paracellular permeability. In a phase II randomized controlled trial, the 0.5 mg dose of larazotide acetate led to a reduction of 50% or more in weekly average celiac disease patient-reported outcome abdominal domain scores from baseline for at least 6 weeks in treated patients compared to placebo (P = 0.029).^[97] Although advanced to phase III trials for celiac disease, development was discontinued in 2022 due to insufficient efficacy endpoints, but earlier data support its role in symptom alleviation alongside a gluten-free diet.^[98] Corticosteroids, such as budesonide, are employed for acute flares in inflammatory bowel disease (IBD) to suppress inflammation and enhance epithelial barrier function. Budesonide, with its topical action in the gut, restores intestinal permeability as measured by the lactulose/mannitol ratio in Crohn's disease patients during active inflammation.^[99] 5-Aminosalicylic acid (5-ASA) derivatives, like mesalamine, stabilize tight junctions in ulcerative colitis by modulating junctional complexes, including upregulation of occludin and claudin-1 expression, thereby reducing epithelial permeability and promoting barrier repair.^[100] Microbial therapies leverage the gut microbiome to reinforce intestinal barrier function through probiotics, synbiotics, postbiotics, and fecal microbiota transplantation (FMT). Probiotics, particularly strains like Lactobacillus rhamnosus GG (LGG) at doses of 10^9 CFU/day, improve epithelial integrity by enhancing tight junction protein expression and reducing permeability in inflammatory conditions. A 2025 systematic review and meta-analysis of 46 randomized controlled trials involving over 3,200 participants demonstrated that probiotics, including LGG, significantly lowered serum zonulin levels (standardized mean difference [SMD] = -0.49, 95% CI: -0.79 to -0.18) and lipopolysaccharide (LPS) (SMD = -0.54, 95% CI: -1.01 to -0.07), indicating reduced intestinal permeability.^[101] Synbiotics, combining probiotics with prebiotics, further bolster barrier function in leaky gut models by promoting beneficial microbial growth and decreasing zonulin release, as evidenced in clinical and preclinical studies where they attenuated stressor-induced permeability increases.^[102] Postbiotics, such as culture supernatants from Lactobacillus reuteri ZJ617, prevent LPS-induced damage to the intestinal barrier by enhancing antioxidant activity and tight junction integrity, mitigating inflammation and permeability in mouse models of acute injury.^[103] Emerging microbial interventions include FMT, which transfers donor microbiota to reshape the recipient's gut ecosystem and repair barrier defects. In IBS with diarrhea (IBS-D), clinical trials from 2023 to 2025 report FMT efficacy rates of 30-65%, defined by at least 30% improvement in IBS severity scoring system scores, with endoscopic or enema delivery showing superior outcomes over placebo in reducing symptoms and permeability markers.^[104]^[105] Preclinical data also highlight arginase-2 inhibitors, such as nor-NOHA, which prevent aging-related intestinal leaks by preserving nitric oxide availability and tight junction stability in rodent models, offering potential for age-associated barrier dysfunction.^[106] These therapies collectively aim to normalize permeability, with efficacy often assessed via urinary sugar ratios or serum biomarkers, complementing broader modulatory strategies.

Controversies and Emerging Research

Leaky Gut Syndrome

Leaky gut syndrome, as described in alternative medicine, refers to a hypothetical condition in which damage to the intestinal lining allows undigested food particles, toxins, and bacteria to "leak" into the bloodstream, purportedly triggering a wide array of symptoms including chronic fatigue, food allergies, skin issues, and joint pain.^[107] This concept gained prominence in the 1980s through nutritionists and alternative health practitioners who promoted it as an underlying cause of diverse health problems, often without established diagnostic criteria or standardized testing protocols.^[108] Proponents typically attribute the syndrome to factors like poor diet, stress, or antibiotic use, suggesting it leads to systemic inflammation and immune dysregulation.^[109] As of 2025, the scientific consensus holds that leaky gut syndrome is not recognized as a formal medical diagnosis, distinguishing it from the well-documented phenomenon of increased intestinal permeability observed in specific gastrointestinal diseases such as celiac disease or inflammatory bowel disease.^[110] While alterations in gut barrier function are real and measurable in certain pathologies, the broader claims of leaky gut syndrome as a standalone entity lack robust evidence, including the absence of validated biomarkers or specific treatments beyond general gut health support.^[111] For instance, proposed therapies akin to unproven interventions like chelation therapy have not demonstrated efficacy in controlled studies for this purported syndrome.^[112] Common misconceptions surrounding leaky gut syndrome include unsubstantiated causal links to autoimmune conditions, where proponents claim toxin leakage directly initiates autoimmunity, despite 2025 reviews emphasizing that such associations require evidence of causation and reliable biomarkers, which are currently absent.^[110] These overreaches often ignore that while intestinal hyperpermeability can contribute to immune responses in established diseases, the syndrome's systemic effects—such as widespread allergies or fatigue—are not supported by mechanistic studies without objective permeability assessments.^[113] In research contexts, the term "leaky gut" is employed descriptively to denote barrier defects in experimental models or clinical conditions, rather than endorsing the syndrome as a diagnostic category.^[114] The public perception of leaky gut syndrome has fueled a substantial market for dietary supplements, including probiotics and glutamine-based products, estimated at over $2.3 billion globally in 2024, driven by direct-to-consumer marketing.^[115] However, randomized controlled trials evaluating these supplements for syndrome-related symptoms generally report benefits no greater than placebo, with only limited evidence for permeability improvements in targeted subgroups, underscoring the need for evidence-based approaches over anecdotal remedies.^[116]

Recent Advances and Future Directions

Recent research has illuminated the intricate connections within the gut-organ axis, particularly the role of intestinal barrier dysfunction in cardiovascular diseases through lipopolysaccharide (LPS) translocation. A 2024 review highlights how impaired gut permeability facilitates LPS leakage, activating systemic inflammation via Toll-like receptor 4 (TLR4) pathways, thereby exacerbating heart failure and atherosclerosis.^[117] Similarly, studies in 2025 have demonstrated that gut microbiota dysbiosis amplifies cardiac remodeling by promoting LPS-induced myocardial inflammation.^[118] In aging populations, microbiota maturation influences rectal and intestinal permeability, with age-related shifts leading to barrier weakening. A 2025 study in aged mice revealed microbiota-dependent increases in intestinal permeability and reduced tight junction protein ZO-1 expression, underscoring the need for microbiome-targeted interventions to mitigate frailty.^[119] Between 2023 and 2025, key highlights include evidence that diet-induced weight loss reverses permeability in metabolic dysfunction-associated steatohepatitis (MASH); a 2024 clinical study showed significant reductions in intestinal permeability markers alongside improvements in MASH histology following caloric restriction.^[95] Postbiotics have emerged as promising agents for barrier maintenance, with a 2025 trial demonstrating that postbiotic supplementation enhances tight junction integrity and reduces inflammation in healthy adults.^[120] Additionally, AI-driven multi-omics approaches are enabling personalized risk assessment, integrating genomic, metagenomic, and metabolomic data to predict permeability-related disease susceptibility.^[121] Despite these advances, critical gaps persist, particularly in distinguishing causal from correlative roles of intestinal permeability in the gut-brain axis and depression. Limited longitudinal data hinder causal inference, as most evidence relies on cross-sectional associations between barrier dysfunction, microbiota alterations, and depressive symptoms.^[65] Beyond zonulin, standardized biomarkers remain underdeveloped; while fecal calprotectin and serum intestinal fatty acid-binding protein (I-FABP) show promise, their validation across diverse populations is inconsistent, complicating clinical translation.^[5] Looking ahead, targeted modulators of tight junctions, such as agents enhancing claudin expression, hold potential for restoring barrier function in inflammatory conditions.^[122] Microbiome engineering via CRISPR offers innovative avenues, enabling precise editing of gut bacteria to bolster barrier integrity and reduce pathogen translocation.^[123] Ongoing clinical trials are exploring preventive strategies in at-risk groups like the elderly, including prebiotic interventions to improve permeability and mitigate age-related inflammation.^[124] Challenges include ethical concerns in human probe studies, where invasive permeability assessments raise issues of participant burden and safety in vulnerable cohorts.^[125] Furthermore, the underrepresentation of diverse ethnic and socioeconomic groups in research limits generalizability, necessitating inclusive cohorts to address global variations in permeability-related diseases.^[126]

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An increased intestinal transcellular permeability found in different disease conditions has been postulated to be critical for the migration of bacteria and ...
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The Intestinal Barrier and Current Techniques for the Assessment of ...
Intestinal permeability is defined as the passage of a solute through a simple membrane and can be measured by recording the passage of permeability markers ...
[6]
Intestinal permeability – a new target for disease prevention and ...
Nov 18, 2014 · Intestinal permeability, which is a feature of intestinal barrier function, is increasingly recognized as being of relevance for health and disease.
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Gut microbiota, intestinal permeability, and systemic inflammation
Jul 28, 2023 · The maintenance of a stable intestinal barrier is crucial to prevent luminal substances and pathogens from entering the internal environment.
[8]
The History of the Intestinal Microbiota and the Gut-Brain Axis - PMC
Dec 15, 2022 · In the 19th century, physicians tended to blame the gastrointestinal system for its influence on mind [1,9]. In the 20th century, scientists ...
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Tight junctions: from molecules to gastrointestinal diseases - NIH
Increased intestinal permeability can be due to tight junction-dependent and tight junction-independent mechanisms. Two tight junction-dependent permeability ...
[10]
Mechanisms regulating intestinal barrier integrity and its ... - Nature
Aug 16, 2018 · Tight junction complexes are the rate-limiting factor for paracellular permeability; they are programmed to rapidly open and seal the barrier in ...
[11]
Intestinal mucus components and secretion mechanisms: what we ...
Apr 3, 2023 · The hierarchical structure of the mucus barrier is key in preventing bacterial penetration and blocking direct contact of bacteria and the ...
[12]
Layered defense: how mucus and tight junctions seal the intestinal ...
Jul 13, 2017 · This review focuses on how intestinal mucus and intercellular tight junctions (TJs) act together to maintain the integrity of the colonic barrier.
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Intestinal barrier permeability: the influence of gut microbiota ...
Mucus and epithelium are the most important components of the intestinal barrier that limit the development of inflammation. The mucous layer consists of two ...
[14]
Imaging therapeutic peptide transport across intestinal barriers - PMC
These pathways include (Fig. 1, right) active transcytosis mediated vesicular transport, passive transcellular transport through epithelial cells, and ...Missing: PEPT1 | Show results with:PEPT1
[15]
Bacterial colonization and intestinal mucosal barrier development
This review summarizes recent advances in understanding the complex ecosystem of intestinal microbiota and its role in regulating intestinal barrier function.
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Intestinal Barrier Function: Molecular Regulation and Disease ...
Transcellular permeability is associated with solute or water movement through intestinal epithelial cells. Paracellular permeability is associated with ...
[17]
Paracellular permeability and tight junction regulation in gut health ...
Apr 25, 2023 · Intestinal permeability can reflect contributions of three distinct pathways: the pore pathway, the leak pathway and the unrestricted pathway ( ...
[18]
Role of Stress on Driving the Intestinal Paracellular Permeability
Nov 18, 2023 · The paracellular route allows the passage of ions and small molecules between the spaces of the lateral membrane of the epithelial cells (4).
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Na(+)-D-glucose cotransporter SGLT1 is pivotal for intestinal ...
Dec 12, 2011 · The primary transporters that mediate transcellular movements of d-glucose in small intestine have been identified and include the Na+-d-glucose ...
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Breaking the Barrier: The Role of Gut Epithelial Permeability in ... - NIH
1). The paracellular and the transcellular endocytosis pathways are the most relevant for intestinal permeability, with most research to date focused on the ...
[21]
Integrative physiology of transcellular and paracellular intestinal ...
Jul 15, 2017 · Transcellular absorption: glucose from the lumen (top of the figure) can move across the epithelium by uptake via the Na+-coupled glucose ...
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Structure-function analysis of tight junction-directed permeation ...
The intestinal epithelium is a semi-permeable barrier that limits the non-selective absorption of hydrophilic molecules with a molecular weight of > ∼500 Da [1] ...
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Determination and prediction of permeability across intestinal ...
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Short-Chain Fatty Acids Manifest Stimulative and Protective Effects ...
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Biological Function of Short-Chain Fatty Acids and Its Regulation on ...
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Glucagon-like peptide-2 enhances intestinal epithelial ... - PubMed
GLP-2 enhances intestinal epithelial barrier function by affecting both paracellular and transcellular pathways and thus may be of therapeutic value.
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Glucagon-Like Peptide-2 Reduces Intestinal Permeability But Does ...
GLP-2 has also been shown to enhance transcellular and paracellular gut barrier function, by increasing transepithelial resistance in the normal gut and after ...
[28]
Glutamine supplementation decreases intestinal permeability and ...
Conclusions: GLN had a positive impact on the intestinal barrier by reducing permeability and bacterial translocation to physiologic levels and preserving ...
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Glutamine and whey protein improve intestinal permeability and ...
Glutamine, the major fuel for the enterocytes, may improve IP. Aim: We evaluated the effect of oral glutamine on IP and intestinal morphology in patients with ...
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Gliadin, zonulin and gut permeability: Effects on celiac ... - PubMed
We concluded that gliadin activates zonulin signaling irrespective of the genetic expression of autoimmunity, leading to increased intestinal permeability to ...
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Interferon-gamma and tumor necrosis factor-alpha disrupt epithelial ...
We found that TNF-alpha and IFN-gamma synergistically affected the epithelial barrier and disrupted the structure of TJs.
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Proinflammatory cytokines disrupt epithelial barrier function by ...
Our findings for the first time clearly separate the proapoptotic effects of IFN-gamma and TNF-alpha from their abilities to disrupt barrier function.
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Relevance of oxidative stress for small intestinal injuries induced by ...
Dec 13, 2024 · Nonaspirin NSAIDs-induced intestinal mucosal injury is associated with antioxidant systems, resulting in increased oxidative stress.
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Evidence that chronic alcohol exposure promotes intestinal ... - NIH
Evidence that chronic alcohol exposure promotes intestinal oxidative stress, intestinal hyperpermeability and endotoxemia prior to development of alcoholic ...
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Psychological stress and corticotropin-releasing hormone increase ...
Acute psychological stress increases small intestinal permeability in humans. Peripheral CRH reproduces the effect of stress and DSCG blocks the effect of ...Missing: CRF | Show results with:CRF
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Psychosocial stress-induced intestinal permeability in healthy humans
Chronic peripheral administration of corticotropin-releasing factor causes colonic barrier dysfunction similar to psychological stress. Am. J. Physiol ...
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Salmonella infection upregulates the leaky protein claudin-2 in ...
We have shown that Salmonella targets the tight junction protein claudin-2 to facilitate bacterial invasion. We speculate that this disruption of barrier ...
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Knockout animals and natural mutations as experimental and ...
Many pathologies of knockout mice and mutations of the claudin and tricellulin genes in hereditary human diseases have been interpreted as defects in TJ ...2.4. Claudin-14 Gene... · 2.6. Claudin-16 Mutations... · 4. Tricellulin Mutations
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Claudin-1 Regulates Intestinal Epithelial Homeostasis through the ...
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How to Perform and Interpret Intestinal Permeability - NIH
Lactulose and mannitol ratio is the most commonly used test for assessment of small intestinal permeability and the most reliable method for measurement of ...Missing: review | Show results with:review<|control11|><|separator|>
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Methods to determine intestinal permeability and bacterial ... - NIH
Intestinal permeability can be assessed through enteral administration of non-digestible markers, which ideally should cross the mucosal barrier by non-mediated ...
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Fecal zonulin is elevated in Crohn's disease and in cigarette smokers
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Zonulin Upregulation Is Associated With Increased Gut Permeability ...
May 1, 2006 · The increased zonulin levels correlated with increased intestinal permeability in vivo and changes in claudin-1, claudin-2, and myosin IXB genes ...
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Local barrier dysfunction identified by confocal laser ... - Gut
Assessment of barrier function and cell shedding by confocal endomicroscopy promises to be a simple, fast and practical method of predicting relapse of IBD.
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Small Intestinal Permeability and Gut-Transit Time Determined with ...
May 28, 2018 · 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability.
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The Ussing chamber system for measuring intestinal permeability in ...
Jun 20, 2019 · The Ussing chamber system can be utilised as a valuable tool for measuring gut integrity. Here we describe step-by-step methodology required to ...
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A guide to Ussing chamber studies of mouse intestine
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Stool multi-omics for the study of host–microbe interactions in ...
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Elevated fecal calprotectin is associated with gut microbial dysbiosis ...
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Zonulin as a Biomarker for the Development of Celiac Disease
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Gliadin Induces an Increase in Intestinal Permeability and Zonulin ...
Gliadin induces an MyD88-dependent zonulin release that leads to increased intestinal permeability, a postulated early element in the pathogenesis of celiac ...
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Intestinal Permeability in Inflammatory Bowel Disease
A defective mucosal barrier may result in increased intestinal permeability which promotes the exposition to luminal content and triggers an immunological ...
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TNF-α-induced increase in intestinal epithelial tight junction ...
These results demonstrate for the first time that TNF-α-induced increase in Caco-2 TJ permeability was mediated by NF-κB activation.
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Hyperglycemia drives intestinal barrier dysfunction and risk for ...
Our results mechanistically link hyperglycemia and intestinal barrier function with systemic infectious and inflammatory consequences of obesity and diabetes.
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The Role for Gut Permeability in the Pathogenesis of Type 1 Diabetes
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Gut permeability is related to body weight, fatty liver disease, and ...
These findings suggest that the Lac:Man ratio could be a valuable marker of intestinal permeability in patients with obesity and metabolic disorders. In ...
[57]
Intestinal Barrier Permeability in Obese Individuals with or without ...
Sep 3, 2022 · Altered intestinal barrier permeability has been associated with obesity and its metabolic and inflammatory complications in animal models.
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Intestinal Permeability in Disorders of Gut–Brain Interaction
Sep 3, 2024 · This review highlights the techniques for assessment of barrier function, appraises evidence for barrier dysfunction in DGBI including mechanistic studies in ...
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Intestinal permeability assessed by serum zonulin in liver cirrhosis
Apr 18, 2025 · It plays a crucial role in the rapid, reversible, and dynamic regulation of intestinal permeability, making it a key marker of this process. In ...
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Serum zonulin levels in patients with liver cirrhosis - NIH
Increased gut permeability and bacterial translocation play an important role in liver cirrhosis. Zonulin is a recently recognized protein involved in the ...
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Celiac Disease and Non-celiac Wheat Sensitivity: State of Art of Non ...
Sep 8, 2020 · Innate and adaptive immune response (IL15, IL8, IELs, Th1/Th17, antibodies production), increased intestinal permeability, Altered expression ...
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Latest In vitro and in vivo models of celiac disease - PMC - NIH
The increased intestinal permeability in celiac disease allows for paracellular transfer of gluten derived peptides to the lamina propria, resulting in the ...
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Role of Metabolic Endotoxemia in Systemic Inflammation ... - PubMed
Jan 11, 2021 · LPS activates Toll-like receptor-4 (TLR4) leading to the production of numerous pro-inflammatory cytokines and, hence, low-grade systemic ...
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A high-fat meal induces low-grade endotoxemia - ScienceDirect.com
Background: Bacterial endotoxin is a potently inflammatory antigen that is abundant in the human gut. Endotoxin circulates at low concentrations in the ...
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Gut dysbiosis-derived low-grade endotoxemia: A common basis for ...
Gut dysbiosis is characterized by bacteria overgrowth that ultimately leads to increased intestinal barrier permeability and translocation of bacteria or ...<|separator|>
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Intestinal permeability in human cardiovascular diseases - Frontiers
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Intestinal Permeability and Depression—A Narrative Review ... - MDPI
There is ample evidence supporting associations between zonulin and mental illness, although findings remain partially inconclusive.Missing: Beck | Show results with:Beck
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Microbiota–gut–brain axis and its therapeutic applications ... - Nature
Feb 16, 2024 · There is a growing recognition of the role of gut microbiome in neurodegenerative diseases. Notably, early microbiome changes were detected in ...
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Serum lipopolysaccharide binding protein (LBP) and metabolic ...
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A review of gut failure as a cause and consequence of critical illness
Feb 26, 2025 · In critical illness, all elements of gut function are perturbed. Dysbiosis develops as the gut microbial community loses taxonomic diversity and new virulence ...
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Wistar Scientists Discover p53 Can “Read” Cellular Signals to Direct ...
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Potential effects of gut microbiota on host cancers: focus on ...
In this review, we discuss how the gut microbiota play a role in human cancer and its application in anticancer therapy.
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Impact of Diet-Modulated Butyrate Production on Intestinal Barrier ...
Oct 13, 2018 · A suppressed production of butyrate is crucial, as this short-chain fatty acid (SCFA) can play a key role not only in colonic health and ...
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High fiber dietary and sodium butyrate attenuate experimental ...
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Can Diet Alter the Intestinal Barrier Permeability in Healthy People ...
Jun 14, 2024 · In respect to infants fed formula or breast milk, those breastfed presented a higher lactulose/mannitol ratio, indicating greater IBP.
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Effect of the Mediterranean diet on the faecal long-chain fatty acid ...
We recently showed that adherence to the Mediterranean diet increased the proportion of plasma n-3 PUFA, which was associated with an improved intestinal ...
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Fermented Foods, Health and the Gut Microbiome - PMC
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Kefir and Intestinal Microbiota Modulation: Implications in Human ...
Feb 21, 2021 · Fermented foods are able to modify the composition of the intestinal microbiota, improve the control of intestinal permeability, increase its ...
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A systematic review and meta-analysis of clinical trials on the effects ...
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A systematic review and meta-analysis of clinical trials on the effects ...
Overall, glutamine supplementation did not significantly affect intestinal permeability, and the meta-analysis demonstrated a significant reduction in ...
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Omega Fatty Acids and Inflammatory Bowel Diseases: An Overview
Sep 30, 2019 · The results showed that ω3 fatty acids reduce intestinal inflammation, induce and maintain clinical remission in UC patients.
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Interplay of n-3 Polyunsaturated Fatty Acids, Intestinal Inflammation ...
In inflammatory bowel diseases (IBDs), such as ulcerative colitis and Crohn's disease, doses between 1000 and 3000 mg per day of EPA and DHA have been shown to ...3. From The Celiac Immune... · 6. Pufas And The Gut... · 7. N-3 Pufa: Effects On The...
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Is There an Exercise-Intensity Threshold Capable of Avoiding ... - NIH
There is some evidence that vigorous endurance training (≥60 min and ≥70% of maximum work capacity) may lead to injury and increased intestinal permeability.
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Intensity-specific considerations for exercise for patients with ...
Feb 20, 2023 · showed that IBD patients had an improvement in intestinal permeability after completing moderate-intensity aerobic exercise as measured by ...
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The effect of a mindfulness-based therapy on different biomarkers ...
Apr 8, 2020 · CRF also increases intestinal permeability by causing mast cells to release tumour necrosis factor alpha and proteases. Thus, targeting CRF ...Methods · Study Design · Discussion
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Psychological stress in inflammatory bowel disease - Frontiers
It has been reported that psychological stress is involved in the permeability, motility, sensitivity, and secretion of the intestine, composition of gut ...
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Markers of Intestinal Permeability Are Rapidly Improved by Alcohol ...
May 14, 2021 · Our data suggest that one-week of abstinence improves markers of intestinal barrier function and liver health in ALD patients.
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Markers of Intestinal Permeability Are Rapidly Improved by Alcohol ...
May 12, 2021 · Serum markers of intestinal permeability in controls and patients with ALD before and after alcohol withdrawal. (A) Zonulin and (B) I-FABP ...
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Changes in intestinal permeability and gut microbiota following diet ...
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Changes in intestinal permeability and gut microbiota following diet ...
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[PDF] Larazotide Acetate for Persistent Symptoms of Celiac Disease ...
CONCLUSIONS: Larazotide acetate 0.5 mg reduced signs and symptoms in CeD patients on a GFD better than a GFD alone. Although results were mixed, this study was ...
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Phase 3 study of larazotide acetate to treat celiac disease ...
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Corticosteroid enhances epithelial barrier function in intestinal ... - NIH
In one previous study, CS was shown to restore an increased intestinal permeability as determined by the urinary lactulose/mannitol ratio in CD patients [14].
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Mesalamine and azathioprine modulate junctional complexes and ...
Feb 26, 2019 · This study provides mechanistic evidence that molecular actions of 5-ASA and AZTP on intestinal epithelia are fundamental in the resolution of barrier ...
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Reinforcing gut integrity: A systematic review and meta-analysis of ...
This systematic review and meta-analysis explores the effects of pro- and synbiotic, or prebiotic administration, on various intestinal permeability markers.
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Human Intestinal Barrier: Effects of Stressors, Diet, Prebiotics, and ...
In summary, probiotics and synbiotics enhance intestinal barrier function in response to stressor or disease states. Future studies should address the changes ...
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Unlocking the power of probiotics, postbiotics: targeting apoptosis ...
Furthermore, the culture supernatant of L. reuteri ZJ617 attenuates lipopolysaccharide (LPS)-induced acute liver injury by strengthening intestinal barrier ...<|control11|><|separator|>
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Efficacy of faecal microbiota transplantation for patients with irritable ...
This study demonstrates the effectiveness of FMT in the treatment of IBS. ... The response criteria for IBS-D are an improvement at least 30% in the ...
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Clinical efficacy and safety of faecal microbiota transplantation in the ...
Sep 18, 2024 · This study suggests that patients with IBS may benefit from FMT especially when it is administered via endoscopy, nasojejunal tube or rectal enema.
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Impairments of intestinal arginine and NO metabolisms trigger aging ...
Intestinal arginine and NO metabolisms could be a target in the prevention of aging-associated intestinal barrier dysfunction and subsequently decline and ' ...
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Leaky Gut Syndrome: Symptoms, Diet, Tests & Treatment
Leaky gut syndrome is a hypothetical condition in which your intestines become permeable to toxins that leak into your bloodstream and trigger systemic ...
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Debunking the Myth of 'Leaky Gut Syndrome' - badgut.org
Feb 20, 2024 · Some alternative medicine practitioners claim that leaky gut syndrome is a prevalent problem responsible for ill health in many people. However ...
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Leaky gut: What is it, and what does it mean for you? - Harvard Health
Sep 12, 2023 · An unhealthy gut lining may have large cracks or holes, allowing partially digested food, toxins, and bugs to penetrate the tissues beneath it.
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Leaky Gut Syndrome: Myths and Management - PMC
Leaky gut syndrome is a condition widely popularized in the lay literature, although it is not currently accepted as a formal medical diagnosis.
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[PDF] Leaky Gut Syndrome: Myths and Management
May 5, 2024 · Abstract: Leaky gut syndrome is a condition widely popularized in the lay literature, although it is not currently accepted as a formal ...<|separator|>
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You Probably Don't Have a Leaky Gut - McGill University
Feb 23, 2024 · Leaky gut syndrome is not a real diagnosis, but if you step through the looking-glass like Alice in Wonderland, it will become real.
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The Myths and Scientific Realities About the Gut Microbiota - MDPI
Leaky Gut Syndrome Is a Clearly Defined Clinical Diagnosis. The phrase leaky gut syndrome is often used in popular media and by alternative health ...
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Leaky gut in systemic inflammation: exploring the link ... - PubMed
Dec 6, 2024 · This review outlines current literature on the leaky gut in aging, how leaky gut might contribute to systemic inflammation, and the links ...
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Leaky Gut Supplements Market Research Report 2033
As per our latest market intelligence, the Global Leaky Gut Supplements market size was valued at $2.3 billion in 2024, and is forecasted to hit $5.1 billion by ...
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Leaky Gut and the Ingredients That Help Treat It: A Review - MDPI
A Randomized Clinical Trial (RCT) with a larger sample size is suggested to confirm the positive benefits of using probiotics and glutamine. Even though ...
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New insights into the intestinal barrier through “gut-organ” axes and ...
In recent years, the concept of the “gut-organ” axis has gained significant popularity, revealing a profound interconnection between the gut and other organs.
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Gut microbiota dysbiosis exacerbates heart failure by the LPS-TLR4 ...
Jul 10, 2025 · Gut microbiota dysbiosis exacerbates heart failure by activating myocardial inflammation through the LPS-TLR4/NF-κB signalling pathway.
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Age-related alterations in gut homeostasis are microbiota dependent
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Postbiotic supplementation promotes gut barrier integrity and ...
Discussion: These findings support the use of postbiotics as a safe and effective nutritional approach to help maintain gastrointestinal and immune health in ...
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Systems Biology of Human Microbiome for the Prediction of ...
Sep 1, 2024 · This mini-review explores the relationship between multiomics, precision medicine, and machine learning to improve our understanding of the gut ...
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Biomarkers of intestinal permeability are linked to incident ...
Lipopolysaccharide causes an increase in intestinal tight junction permeability in vitro and in vivo by inducing enterocyte membrane expression and localization ...<|control11|><|separator|>
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Present and Future Therapeutic Approaches to Barrier Dysfunction
Similarly, in colonic cell lines, EcN EV promoted upregulation of ZO-1 and claudin-14, and induced IL-22 expression reinforcing the intestinal barrier (186).
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CRISPR-Assisted Probiotic and In Situ Engineering of Gut Microbiota
May 16, 2025 · The CRISPR/Cas system offers a promising approach to targeted gut microbiome manipulation. There are two major strategies to edit microbiota ...
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Study Details | Pectin, Aging and Intestinal Barrier Function
Jul 15, 2016 · As intestinal barrier function may be compromised in the elderly we will investigate whether the effects of pectin on selected parameters of gut ...
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Assessment of the Effect of Intestinal Permeability Probes (Lactulose ...
Aug 6, 2025 · Study protocol submitted to Massey University Human Ethics Committee. (PDF). S3 File. Information sheet given to participants.
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Challenges in IBD Research 2024: Preclinical Human IBD ... - NIH
We provide a comprehensive overview of current gaps in inflammatory bowel diseases research that relate to preclinical research and deliver actionable ...