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Culicoides

Culicoides is a of small, hematophagous biting midges in the family (order Diptera), comprising over 1,400 described that are distributed worldwide across diverse habitats. These tiny , typically measuring 1–3 mm in body length, are characterized by their delicate bodies, long antennae, and patterned wings, with only females feeding on blood using a short, downward-pointing . Known as no-see-ums or punkies due to their minuscule size and painful bites, Culicoides play a critical role as vectors for several arboviruses and parasites, posing significant medical, veterinary, and economic threats. Taxonomically, the genus Culicoides belongs to the subfamily Ceratopogoninae and is divided into approximately 33 subgenera, encompassing the majority of its , while the remainder are placed in unclassified species groups or await further phylogenetic resolution. Identification at the species level relies primarily on morphological features such as venation patterns, antennal segmentation, and palpal structures, though molecular methods are increasingly used to address cryptic . With ongoing discoveries, the total number of species is estimated to exceed 1,400 as of 2023, reflecting the genus's vast and challenges in classification. Ecologically, Culicoides species thrive in humid, temperate to tropical environments, breeding in a variety of moist, organic-rich substrates including along edges, decaying , litter, and animal piles. Larvae develop in these semi-aquatic or damp terrestrial sites, often influenced by presence, which provides both breeding media and hosts, while adults are crepuscular fliers that seek hosts near bodies or shaded areas. Their global distribution spans all continents except , with abundance peaking in warmer seasons and regions with high rainfall, though some species are adapted to arid or forested niches. The medical and veterinary significance of Culicoides stems from their capacity to transmit pathogens such as bluetongue virus (BTV) and virus (EHDV) to ruminants, leading to outbreaks that cause substantial mortality and trade restrictions. In humans, species like C. paraensis vector in the , with recent outbreaks from 2022–2025 causing thousands of cases of febrile illness, while bites from various species can induce allergic reactions, including equine insect hypersensitivity (sweet itch) in horses. Economically, vector-borne diseases facilitated by Culicoides have prompted extensive surveillance, control measures like applications, and research into their to mitigate impacts on agriculture and public .

Taxonomy and phylogeny

Classification

Culicoides is a genus of biting midges belonging to the family Ceratopogonidae within the order Diptera. This family encompasses small hematophagous flies, with Culicoides representing the largest genus, comprising over 1,300 described species worldwide as of recent catalogs. The genus was formally established by Pierre André Latreille in 1809, building on earlier species descriptions such as Culicoides punctatus by Johann Wilhelm Meigen in 1804, initially placed under Ceratopogon. Early 20th-century taxonomy saw initial checklists by Jean-Jacques Kieffer in 1906, which suffered from unclear generic boundaries, followed by stabilization through Frederick Wallace Edwards' work in 1926. Comprehensive regional catalogs emerged in the mid-20th century, including those by Philippe Arnaud in 1956 and collaborative efforts by Arnaud and Willis Wirth in 1964, culminating in global revisions by Borkent and Wirth in 1997 and ongoing updates by Borkent in 2014. Modern revisions continue to refine classifications through integrated morphological and genetic approaches, addressing cryptic diversity and subgeneric monophyly. The record of Culicoides extends back to the mid-Cretaceous, with the oldest known specimens represented by four extinct preserved in dating to approximately 99 million years ago. These early s indicate that hematophagous lineages within the genus were already diverse during the . In total, approximately 50 extinct have been documented, primarily from deposits. Taxonomic debates surrounding the validity of Culicoides as a distinct arise from morphological similarities with related genera such as Leptoconops, particularly in wing venation and antennal structures, which complicate delineations in both extant and forms. Molecular phylogeny studies since 2017 have further illuminated these relationships, supporting the genus's while highlighting challenges in species-level distinctions.

Subgenera and species complexes

The genus Culicoides encompasses over 1,300 described , distributed across more than 30 recognized , with ongoing taxonomic revisions incorporating molecular data to refine these groupings. Among these, the Avaritia stands out as monophyletic, comprising approximately 100 that include major vectors of the in ruminants, such as C. imicola and members of the Obsoletus complex. In contrast, the Oecacta, which contains around 180 and serves as a repository for many morphologically similar taxa, has been shown to be paraphyletic through phylogenetic analyses, highlighting the need for further subdivision. Species complexes within Culicoides represent clusters of morphologically cryptic taxa that pose significant challenges for identification and surveillance. The Obsoletus complex, a key group in the subgenus Avaritia, primarily includes C. obsoletus, C. scoticus, and C. chiopterus as closely related implicated in transmission across ; molecular phylogenies from 2017 explicitly exclude C. dewulfi from this , placing it in a distinct . Other notable complexes, such as those in the subgenera Monoculicoides and Haematomyidium, similarly reveal sister with overlapping distributions and ecological roles. Recent advancements in , particularly studies published in 2025, have uncovered additional cryptic diversity within these complexes, identifying multiple molecular operational taxonomic units (MOTUs) in species like C. actoni (three MOTUs) and C. clavipalpis (four MOTUs) from Southeast Asian populations. These findings underscore the limitations of alone, with intraspecific exceeding 18% in some cases, necessitating integrative that combines genetic markers (e.g., sequencing) with morphological and ecological data for accurate delimitation. Such approaches are essential to address the high cryptic diversity in Culicoides, which complicates epidemiological monitoring of vector-borne diseases.

Physical characteristics

Adult morphology

Adult Culicoides are small hematophagous flies measuring 1 to 5 mm in length, with slender bodies covered in fine hairs that contribute to their delicate appearance. The body is divided into a head, , and , with the overall coloration typically dark brown to black, though variations occur across species. The head features contiguous compound eyes and prominent sensory structures essential for host detection. Antennae consist of 15 segments, appearing more plumose and elongated in males due to dense whorls of setae, while females have shorter, less hairy antennae with sensilla coeloconica distributed on specific flagellomeres (e.g., segments 4–6 in mammalophilic species). Maxillary palps are five-segmented, with the third segment enlarged in females and equipped with sensory pits containing basiconica sensilla sensitive to . The proboscis is elongated and piercing, comprising a stylet-like , toothed mandibles, maxillae, hypopharynx, and labium, enabling blood-feeding primarily in females. Wings are held roof-like over the at rest and exhibit characteristic mottled patterns formed by pale spots along radial and cross veins, aiding in species identification; these patterns arise from macrotrichia distribution and pigmentation. The wings feature a reduced number of veins, with the extending beyond the wing tip in many , and radial cells that vary in . In C. circumscriptus, geometric morphometric analyses reveal intraspecific variations in wing and , with larger wings in northern populations (e.g., mean landmark of 1.887 mm) potentially enhancing flight capabilities for dispersal and competence in transmitting pathogens like bluetongue . Legs are long and slender, with horn-shaped tarsal claws and spurs on the tibiae, while the is segmented and often bristled ventrally. Sexual dimorphism is pronounced, particularly in mouthparts and sensory organs: females possess robust mandibles with up to 16 fine teeth for piercing skin, contrasting with males' reduced mandibles bearing only 2–3 teeth, rendering them non-biting. Males exhibit more feathery antennae for detection during mate location, whereas females' palps and antennae are adapted for host-seeking via olfactory cues like CO₂ and .

Immature stages

The eggs of Culicoides species are elongated and cigar-shaped, typically measuring less than 0.5 mm in length, with a surface covered in fine, randomly distributed projections known as ansulae that may aid in or . They are laid in clusters on damp , soil, or decaying near water bodies, featuring a sticky coating that ensures attachment to substrates in semi-aquatic environments. Upon oviposition, the eggs are pale but rapidly darken to brown or black, enhancing in moist habitats. Larvae exhibit a worm-like, elongated body form, reaching up to 7 mm in length in the final , and are adapted for life in semi-aquatic , , or tree holes where they feed on microorganisms and smaller . They possess sclerotized head capsules for protection and predation, with a translucent body often revealing dark gut contents from ingested ; occurs via an anal or gills that allow access to atmospheric oxygen in low-oxygen substrates. Development proceeds through four , with early instars featuring a or pseudopod on the first thoracic segment to facilitate movement across wet surfaces, while later instars develop robust mandibles and epipharyngeal combs for tearing prey. Pupae are of the exarate type, approximately 2–3 long, with free appendages and a spiny that provides defensive structures against predators in moist soils or debris. They are enclosed within silken cocoons spun in damp substrates, offering and humidity retention essential for survival in semi-aquatic conditions. Respiratory trumpets, or horns, project from the , featuring crenulated and scaled surfaces with multiple spiracular openings to facilitate air intake while submerged bases exclude water. Identification of immature Culicoides stages relies on microscopic examination of setation patterns—such as the arrangement and number of setae on the head and body—and siphonal structures, including the shape and dentition of the dorsal comb and epipharynx, which vary across species and instars. These features enable differentiation in ecological surveys of breeding sites.

Distribution and habitat

Global range

Culicoides species exhibit a , occurring on nearly all continents except , with over 1,400 described species worldwide as of 2025. The genus demonstrates highest in tropical regions, where environmental conditions support diverse habitats; for instance, the Neotropics harbor approximately 299 species, reflecting adaptations to varied ecosystems in Central and . In temperate zones, Culicoides achieve near-cosmopolitan coverage, though with lower diversity, as populations persist in cooler climates through overwintering strategies and opportunistic breeding sites. In , the Obsoletus complex, comprising species such as Culicoides obsoletus and C. scoticus, dominates the Culicoides fauna, with widespread presence across the continent from northern to southern Mediterranean countries. The feature notable regional specialists, including C. sonorensis in the , where it thrives in arid and semi-arid landscapes, extending into . hosts significant vector species like C. imicola, which is prevalent across sub-Saharan regions and serves as a primary transmitter of arboviruses in savanna and semi-arid zones. In , Culicoides diversity spans multiple subgenera, with over 160 recorded in alone, encompassing subgenera such as Avaritia and Meijeringia, adapted to monsoon-influenced and tropical forest environments. Human-mediated dispersal has facilitated the introduction of Culicoides species to new areas, often via and transport networks such as shipping. For example, living Culicoides individuals, including potential non-native species, have been intercepted on vessels arriving at ports in regions like , highlighting risks of inadvertent spread through global commerce. Post-2000, expansions of established species like C. obsoletus into previously marginal European areas coincided with increased trade and viral outbreaks, though direct trade-linked introductions remain understudied for this . Climate change is driving range expansions for Culicoides, with warming temperatures enabling poleward shifts in distribution. Species distribution models project northward advancements in and under future scenarios, such as those from , potentially increasing suitable habitats by 20-50% in temperate latitudes by mid-century. Recent 2025 analyses for species like C. punctatus confirm these trends, predicting enhanced suitability in higher latitudes due to prolonged active seasons and altered patterns.

Environmental preferences

Culicoides species primarily breed in moist, organic-rich substrates that support larval development, such as dung piles, swampy soils, river banks, floodplains, and reed belts. These sites often feature high moisture content, elevated , and acidic conditions (pH strongly to very strongly acidic), favoring environments conducive to stages. Additional breeding habitats include tree holes and salt marshes, where semi-aquatic or saturated soils provide suitable oviposition cues. Microhabitat preferences vary among species, reflecting adaptations to local conditions within broader distributions. For instance, , part of the variipennis complex, thrives in arid or saline soils and mudflats, often associated with alkaline environments in semi-arid regions. In contrast, favors coastal dunes and salt marshes, particularly in temperate coastal zones, where it exploits brackish, organic-enriched sediments. Abiotic factors significantly influence Culicoides activity and survival, with optimal temperatures ranging from 15°C to 25°C for peak abundance and development. High relative , typically above 50-70%, enhances adult activity and larval viability, while lower levels can limit dispersal and reproduction; and also modulate suitability by altering and substrate stability. Photoperiod affects seasonal patterns, with longer day lengths promoting emergence in spring and summer. Biotic interactions further shape habitat preferences, including associations with hosts that influence site selection. like Culicoides obsoletus are closely linked to , near heaps in grazed pastures where host proximity facilitates feeding and oviposition. Other rely on wild ungulates or birds, with regimens altering microbial communities in , thereby affecting larval and . Recent studies highlight the of microbiomes in these dynamics; for example, gut bacteria in Culicoides sonorensis and C. nubeculosus modulate competence and environmental , while larval microbiomes driven by factors like host influence composition and abundance.

Life cycle and development

Egg and larval stages

Female Culicoides typically require a to develop eggs, after which gravid females oviposit batches containing 35 to over 100 , depending on species and environmental conditions. Eggs are elongate and banana-shaped, measuring approximately 0.4 mm in length, and are laid in clusters on moist substrates such as , decaying , or organic-rich soils near sites. Oviposition is stimulated by cues from larval habitats, including the presence of and , which gravid females preferentially select for deposition. Egg incubation periods vary with , typically lasting 1-3 days at 25°C to 28°C, with hatching rates often exceeding 50% under optimal conditions. is influenced by environmental factors such as and ; high substrate moisture promotes successful embryonation, while at low relative (e.g., below 75%) can prevent even in older eggs. Upon hatching, larvae emerge into semi-aquatic environments where they undergo four instars, feeding primarily on microorganisms like , fungi, and nematodes within mud or organic detritus. Larval development duration ranges from 5-10 days at warmer (26-33°C) to 15-29 days at cooler ones (around 20°C), with total immature development from to accelerated by higher temperatures up to an optimal threshold. In temperate regions, final-instar larvae of many Culicoides species enter during winter, ceasing development until rising spring temperatures trigger resumption, which synchronizes adult emergence with favorable conditions. Early life stages face significant mortality from environmental stressors and biotic factors, including parasitism by mermithid nematodes, which can infest over 50% of larvae in some populations, leading to host death upon nematode emergence. Predatory interactions within breeding sites, such as among late-instar larvae or consumption by other , further contribute to high attrition rates during development.

Pupal stage and adult emergence

The pupal stage of Culicoides follows the completion of the fourth larval instar and occurs within a silken constructed from larval secretions and environmental debris, providing some protection but remaining vulnerable to environmental stresses. This non-feeding phase typically lasts 3–7 days, during which the pharate adult forms inside the pupal , undergoing without further nutrient intake. The duration can extend to 3–4 weeks under cooler temperatures or species-specific conditions, but is generally brief to minimize exposure. Adult emergence, or eclosion, begins when the pharate adult splits the pupal integument along a characteristic straight dorsal slit, allowing escape from the cocoon. This process often occurs in the morning hours, with peak activity between approximately 07:00 and 12:00 for species like C. furens. Upon emergence, the soft-bodied adults pump hemolymph into their wings, causing expansion, followed by sclerotization and hardening within a few hours, enabling flight capability. The sex ratio at emergence is frequently near 1:1, as observed in field collections from breeding sites, though slight biases toward females or males can occur depending on species and local conditions. Several factors influence the success of pupation and , with playing a key role in developmental rate and survival. Higher temperatures accelerate the pupal period—for instance, thresholds around 16.5°C are required for pupal in species like C. brevitarsis, and durations shorten significantly above 20°C, often to about 3 days under optimal lab conditions. Predation risks remain high during this immobile stage, as pupae in exposed cocoons are susceptible to ground-dwelling predators such as and , contributing to substantial pre-adult mortality. Generational cycles vary by climate and species, with overlapping generations in tropical regions enabling up to 9–11 cycles per year for multivoltine species like C. variipennis. In contrast, many temperate species exhibit univoltine patterns, completing only one generation annually due to and cold-induced developmental arrest.

Behavior and ecology

Feeding habits

Adult Culicoides exhibit sexually dimorphic feeding habits, with females being hematophagous and requiring blood meals from hosts for egg maturation, while males feed solely on and juices as their energy source. Both sexes consume plant-derived sugars for general sustenance, but only females engage in blood-feeding to support . Female Culicoides display opportunistic preferences, feeding on a range of vertebrates including mammals, , and reptiles, with patterns influenced by availability in the . analyses from various regions confirm this breadth; for example, in agricultural settings near , over 70% of identified blood sources in engorged females are from and other domesticated mammals, alongside smaller proportions from and wild mammals. In sylvatic areas, reptiles such as serve as occasional s for certain species. Blood-feeding involves the female's short, stout , adapted with sharp mandibles and maxillae that lacerate to form a blood pool for lapping, often aided by salivary anticoagulants. This process typically elicits a wheal-and-flare reaction in hosts, manifesting as immediate and from and release triggered by bite and . Peak biting activity occurs during crepuscular periods, with highest rates around and a secondary surge at dawn, corresponding to optimal environmental conditions for host location. Host-seeking relies on integrated sensory cues, including (CO2) from host , which markedly increases attraction and trap captures by 2- to 12-fold depending on and type. Additional attractants encompass host , which enhances short-range , and visual stimuli like dark shapes or silhouettes that draw females from afar. Swarming near hosts amplifies these behaviors, with females often feeding in groups that overwhelm host defenses through mass attacks and allow multiple individuals to access feeding sites efficiently. Certain Culicoides species are non-hematophagous, characterized by reduced or absent mandibular teeth that preclude blood-feeding, and instead derive nutrition primarily from plant juices and nectar. For instance, Culicoides loisae in some subgenera sustains itself on floral resources, mirroring the diet of males in hematophagous congeners.

Mating and reproduction

Culicoides species exhibit lekking swarms as their primary mating system, where males aggregate in aerial displays near breeding sites or visual landmarks such as vegetation edges or water bodies to attract females. These swarms, often forming in low light conditions like dawn or dusk, allow males to compete for female attention through rapid flight maneuvers. Males detect incoming females primarily via differences in wing beat frequencies, with male antennae tuned to resonate at female-specific tones for species recognition and interception during flight. Contact pheromones, such as those extracted from female abdomens in species like Culicoides melleus, further stimulate male mating responses upon physical contact, enhancing copulation initiation. Insemination occurs immediately following swarm interception, with copulation lasting several minutes during which is transferred via a to the female's spermathecae. Females typically only once, storing viable for use across multiple reproductive bouts, which supports egg fertilization without remating. Previously mated females show strong resistance to additional suitors, minimizing energy costs associated with repeated copulation. Fecundity in female Culicoides is characterized by 2–4 gonotrophic cycles per individual, each requiring a to initiate yolk deposition and egg maturation. A single typically yields 50–200 eggs per cycle, depending on species and nutritional quality, with oviposition occurring in moist substrates near larval habitats. Mating success in Culicoides populations is density-dependent, with higher swarm densities near sites increasing male-female encounter rates but also intensifying intrasexual among males. Studies as of 2022 indicate that rising temperatures due to can elevate reproductive rates by shortening gonotrophic cycle durations (e.g., from 4.5 days at 20°C to 2.6 days at 30°C) and boosting overall population abundance in optimal ranges (25–30°C), potentially amplifying vector capacity in affected regions. As of 2024, warming has been linked to expanded habitats and reduced extrinsic periods for pathogens, further enhancing risks.

Disease transmission

Vector competence

Vector competence refers to the intrinsic ability of Culicoides species to acquire, maintain, and transmit pathogens biologically, rather than mechanically. In competent species, arboviruses such as orbiviruses are ingested during a from an infected host, initially infecting and replicating within the epithelial cells of the midge's . The must then overcome a midgut escape barrier to disseminate into the hemocoel, from where it reaches and replicates in the salivary glands, enabling transmission to a new host via subsequent bites. The extrinsic (EIP), the time required for the to become transmissible after , typically lasts 10-14 days for orbiviruses in Culicoides under moderate temperatures, during which and occur primarily in the salivary glands. This period is critical, as midges must survive long enough post- to feed again. In non-competent or less efficient , barriers such as failure of or escape prevent viral , limiting potential. For instance, Culicoides imicola exhibits high for orbiviruses, supporting efficient replication and , while many northern European like C. obsoletus show lower susceptibility due to stronger barriers. Several factors influence vector competence, including environmental temperature and the dose of ingested . Optimal temperatures around 25°C accelerate and shorten the EIP, enhancing overall efficiency, whereas higher temperatures above 30°C can increase competence but reduce survival. A higher ingested dose also boosts infection and rates by overcoming initial barriers more readily. Experimental infections demonstrate this variation, with rates ranging from 20% to 80% across Culicoides species; for example, C. sonorensis achieves up to 80% to salivary glands for bluetongue virus, while rates in C. imicola vary similarly depending on and conditions.

Major pathogens transmitted

Culicoides midges serve as vectors for several significant pathogens, predominantly affecting veterinary health through the transmission of viruses, parasites, and . These pathogens primarily impact and , leading to substantial economic losses in and efforts worldwide. Human infections are primarily limited to allergic responses from bites and emerging arboviral diseases like Oropouche fever, while certain parasites pose zoonotic risks in tropical regions. Among the viruses transmitted by Culicoides, bluetongue virus (BTV) stands out as a major orbivirus causing severe disease in ruminants such as sheep, goats, and cattle. BTV infection results in symptoms including high fever, oral lesions, lameness, and respiratory distress, with mortality rates up to 30% in susceptible sheep populations. Epizootic hemorrhagic disease virus (EHDV), another orbivirus, primarily affects deer and other cervids, inducing hemorrhagic fever characterized by vascular damage, edema, and sudden death, often decimating wildlife herds during outbreaks. African horse sickness virus (AHSV), also an orbivirus, causes a highly fatal disease in equids with mortality rates up to 90%, featuring respiratory distress, fever, and edema. Schmallenberg virus, an orthobunyavirus, targets pregnant livestock like cattle and sheep, leading to congenital defects such as arthrogryposis, hydranencephaly, and brachygnathia in offspring, which can result in high rates of abortion or stillbirths. In humans, Culicoides paraensis transmits Oropouche virus (OROV), an orthobunyavirus causing Oropouche fever with symptoms including fever, , muscle pain, and ; outbreaks from 2023 to 2025 have reported over 20,000 cases across the , including non-endemic areas, with rare fatalities and potential for . Parasitic pathogens vectored by Culicoides include filarial nematodes of the genus Onchocerca, which cause bovine or in cattle. These parasites lead to dermal microfilariae accumulation, resulting in nodules, pruritus, and secondary that reduce hide quality and production. In tropical areas, Culicoides also transmit Mansonella species, such as Mansonella ozzardi, contributing to human zoonotic with mild symptoms like and joint pain, though often . Transmission of and by Culicoides is less prevalent but notable for avian haemoproteids like Haemoproteus , which infect birds and cause , , and reduced fitness in wild and domestic populations. such as Culicoides imicola and C. obsoletus are key vectors for these diverse pathogens across various hosts. Overall, the host range emphasizes veterinary significance for and , with exposure primarily eliciting allergic reactions or arboviral infections rather than systemic parasitic diseases.

Notable outbreaks and epidemiology

Bluetongue virus in Europe

The 2006-2009 outbreak of bluetongue virus (BTV) serotype 8 in marked the first major incursion of the into temperate regions previously considered unsuitable due to climatic constraints. Initial cases were reported in August 2006 in the and , rapidly spreading to , , and the by the end of the year, with subsequent waves affecting over 12 countries across the continent. The dominant serotype, BTV-8, primarily impacted sheep and , causing clinical signs such as fever, oral lesions, and lameness, with sheep experiencing higher mortality rates than . Over the course of the epidemic, more than 2 million ruminants were infected, leading to significant concerns and direct losses from mortality and reduced productivity. Culicoides species from the Obsoletus and Scotticus complexes, particularly C. obsoletus and C. scoticus, served as the primary vectors during this outbreak, differing from the typical southern European vector C. imicola. These Palearctic midges, abundant in farm environments, facilitated BTV transmission through their biting activity on ruminants. A key factor enabling the outbreak's persistence was a climatic , including an unusually warm summer in 2006 and mild winter conditions, which allowed vector populations to survive and support BTV overwintering—likely through low-level persistent infections in adult midges or alternative mechanisms—contrasting with the expected annual extinction of vectors in northern latitudes. The epidemic inflicted severe economic consequences, with total losses exceeding €1 billion across due to animal deaths, decreased milk and meat production, trade restrictions, and control measures. In response, vaccination campaigns were initiated in 2007 using inactivated BTV-8 vaccines, achieving high coverage in affected herds and significantly reducing incidence by 2008-2009. The outbreak prompted enhanced surveillance protocols, culminating in the establishment of coordinated EU-wide monitoring networks by 2010, including vector trapping and serological testing, to enable early detection of future incursions.

Other regional impacts

In the , epizootic hemorrhagic disease virus (EHDV) outbreaks have significantly impacted populations, particularly during the late 1990s and early . A notable epizootic in 1999, driven by EHDV serotype 1, affected deer in the , marking one of the earliest major recorded events with high mortality rates in affected herds. Culicoides sonorensis serves as the primary vector for EHDV transmission in , facilitating outbreaks through its biting activity on ruminants. Subsequent events in the , including a severe 2012 outbreak in that resulted in over 14,000 deer deaths, underscored the virus's endemic presence and potential for rapid spread in susceptible wildlife populations. In , the first molecular and serological detection of EHDV occurred in from in 2019, with 9.8% seropositivity and approximately 9% RNA detection rates near the U.S. border, indicating transboundary incursions and heightened epidemiological risks for regional and wildlife. In and , Culicoides imicola remains the dominant vector for bluetongue virus (BTV) transmission, sustaining ongoing outbreaks that severely affect sheep flocks. Across , including , , , and , C. imicola facilitates BTV circulation, leading to acute disease with 70-80% mortality in infected sheep, characterized by fever, hemorrhages, and abortions in pregnant ewes. In , while direct isolations from C. imicola are limited, the species' presence in regions like , , and contributes to persistent BTV endemicity in sheep and other ruminants, with related vectors such as C. schultzei amplifying transmission. These ongoing dynamics highlight the role of environmental factors in maintaining BTV reservoirs, resulting in substantial economic losses for communities reliant on sheep husbandry. The emergence of Oropouche virus in from 2023 to 2025 has marked a significant uptick in cases, vectored primarily by Culicoides paraensis midges. 16,239 confirmed cases were reported across the in 2024, with accounting for the majority, including expansions into non-endemic areas and four fatalities, alongside potential links to adverse outcomes like fetal death and . By August 2025, the outbreak continued, with 12,786 additional confirmed cases in the for the year, and at least five fatalities reported, emphasizing the virus's growing threat in urbanizing Amazonian regions where vector density supports rapid dissemination. Recent studies have confirmed , with cases of fetal deaths and congenital anomalies such as . In , Akabane virus outbreaks pose recurrent threats to , transmitted by Culicoides such as C. brevitarsis, with variations driving range expansions. Historical epidemics, including severe events in the 1970s and ongoing sporadic occurrences, have caused congenital defects like and in calves, leading to high rates and economic impacts on . Favorable climatic conditions, such as increased rainfall and shifts, have facilitated northward and southward dispersal of vectors, correlating with elevated seroprevalence in northern and eastern populations during monitoring periods like 2023-2024. Zoonotic risks from Mansonella ozzardi infections are prominent in Amazonian communities, where Culicoides midges transmit the filarial nematode, resulting in high prevalence rates among and populations. In Ecuador's Amazon region, surveys have documented infection rates of 5.2-36.5%, with overall prevalence reaching 20.5% in samples, often overlapping with malaria-endemic zones and complicating clinical management. Transmission by Culicoides , alongside Simulium blackflies, sustains local cycles, posing underrecognized burdens through or mild symptoms like , though long-term effects remain poorly studied in affected communities.

Control and management

Surveillance methods

Surveillance of Culicoides populations relies on standardized techniques to estimate abundance, , and activity patterns of these biting midges, which are critical for assessing vector risk in and wildlife areas. Common methods include ultraviolet (UV) light traps, such as the Centers for Disease Control and Prevention (CDC) downdraft traps, which use UV light to attract and a fan to collect specimens into a container, effectively capturing a broad range of species during nocturnal activity peaks. samplers, often handheld or backpack-mounted, are employed near host animals like or sheep to directly collect host-seeking females, providing insights into vector-host interactions that light traps may miss. Downdraft traps, including variants like the Onderstepoort Veterinary Institute (OVI) design, enhance collection efficiency by directing airflow downward, yielding higher abundances compared to incandescent bulb alternatives in farm settings. These traps are typically operated from dusk to dawn, with collections preserved in for subsequent analysis, though their efficacy varies by and season. Species identification within Culicoides is essential due to the existence of species complexes with similar morphologies, and traditional methods use morphological keys based on wing patterns, palpal segments, and genitalia structures for adult females and males. For challenging identifications, (PCR) assays target genetic markers like the subunit I (COI) to differentiate species within complexes such as the Obsoletus group. Advances in have enabled rapid field ; a 2025 study in used COI-based to identify 25 Culicoides species across five subgenera, achieving an 82.2% success rate in matching sequences to the BOLD database. These molecular tools complement , particularly for immature stages or damaged adults, and support surveillance in remote areas. Pathogen detection in surveilled Culicoides focuses on screening for viruses and other agents to gauge transmission risk, often using quantitative PCR (qPCR) on pooled samples of 10–200 midges to detect nucleic acids from pathogens like bluetongue virus (BTV). Real-time reverse transcription qPCR (RT-qPCR) assays, optimized for sensitivity, identify BTV serotypes in field-collected pools, with limits of detection as low as 10 viral copies per reaction, facilitating early warning in outbreak-prone areas. Blood meal analysis, via PCR amplification of host-specific DNA (e.g., cytochrome b gene), tracks feeding preferences, revealing that species like C. imicola primarily feed on ruminants but also opportunistically on equines and humans, informing host-vector dynamics. Coordinated networks enhance Culicoides surveillance through and standardized protocols across regions. In , VectorNet, a joint initiative by the European Centre for Disease Prevention and Control (ECDC) and the (EFSA), collects and maps distribution data for 10 priority Culicoides species using light trap from over 150,000 records, with annual updates incorporating 20,000 new entries validated by entomological experts. As of 2025, VectorNet's Entomological Network aims to provide entomological expertise for ECDC and EFSA risk assessments of vector-borne diseases, with ongoing efforts to upload validated data for distribution tracking. National programs, such as France's optimized network operating weekly traps in 24 zones since 2016, feed into these efforts, providing spatio-temporal data for predictive modeling. Globally, similar systems integrate with initiatives like the U.S. Midwest Vector and Aerosolized Disease for harmonized protocols, though European frameworks remain the most comprehensive for Culicoides-borne diseases.

Prevention strategies

Prevention strategies for Culicoides biting midges emphasize (IPM) approaches that combine environmental modifications, protective measures, biological agents, and regulatory actions to minimize biting rates and interrupt disease transmission cycles. These strategies target both larval and adult stages, leveraging data to identify high-risk areas and optimize interventions. Habitat management focuses on disrupting larval development sites, which are often moist substrates like , decaying , and animal waste. Draining standing water from marshes, swamps, leaky pipes, and cattle troughs reduces breeding habitats for such as Culicoides sonorensis, with historical applications in North dairy farms showing potential declines. For Culicoides breeding in cattle dung, such as C. dewulfi and C. chiopterus, treating with insecticides like pyrethroids or insect growth regulators (e.g., dimilin at 0.5 ) can suppress emergence by up to 90% in treated substrates. These methods require ongoing farm maintenance to prevent reaccumulation of suitable habitats, though challenges include high implementation costs and variable efficacy in large-scale settings. Personal and livestock protection measures aim to reduce direct exposure to bites. Repellents containing provide effective short-term deterrence against Culicoides, with formulations achieving up to 95% repellency for several hours when applied to skin or clothing. For livestock, -based pour-ons or sprays (e.g., 4% at 30–40 mL per animal) significantly lower landing rates and alleviate symptoms like sweet itch in horses, with reductions observed in 86% of treated cases. Physical barriers, such as fine-mesh screens (no-see-um rated) on windows, doors, and stables, prevent entry, while insecticide-impregnated netting (e.g., with 5% or 6% ) offers extended protection by achieving 100% mortality for up to 27 days. against bluetongue virus (BTV) remains the cornerstone for preventing arboviral outbreaks in ruminants, as it directly targets pathogen transmission without relying on alone, though timely deployment is critical during vector seasons. Biological controls harness natural enemies to suppress populations sustainably. Predators like spiders have been documented preying on adult Culicoides, with molecular gut content analysis confirming predation events in field settings, contributing to localized reductions in midge abundance as part of broader IPM. Entomopathogenic fungi, such as Metarhizium anisopliae and Beauveria bassiana, demonstrate high virulence against larvae and adults; lab and semi-field trials show 80–100% mortality within 5–7 days when applied to resting sites or breeding media, with minimal non-target effects on beneficial insects. Emerging techniques, such as the sterile insect technique (SIT), have been proposed for Culicoides, involving mass-rearing and release of irradiated males to suppress populations without chemical residues, showing promise in pilot studies for related dipterans. Policy frameworks support prevention through regulatory enforcement and adaptive practices. protocols during BTV outbreaks, as outlined by the (WOAH), restrict animal movements in affected zones to contain vector-mediated spread, with U.S. guidelines emphasizing rapid zoning and premises isolation to limit economic impacts. Climate-adaptive farming strategies, such as enhanced drainage systems and diversified land use to minimize moist breeding sites, help mitigate risks from shifting vector distributions under warming conditions, integrating into broader agricultural resilience plans.