Fact-checked by Grok 2 weeks ago

Cytopathic effect

The cytopathic effect (CPE) refers to the degenerative morphological and physiological changes in host cells induced by viral infection, particularly during productive replication of cytocidal viruses, which often culminate in cell lysis or death.^[1] These alterations are a hallmark of many viral infections and serve as visible indicators of viral presence in cell culture systems.^[2] Common manifestations of CPE include cell rounding and detachment from the substrate, formation of syncytia (multinucleated giant cells resulting from membrane fusion), and the accumulation of inclusion bodies—either nuclear or cytoplasmic aggregates of viral components.^[1] For instance, viruses such as herpes simplex virus promote syncytium formation through the action of viral fusion proteins, while adenoviruses produce crystalline inclusion bodies in the nucleus.^[1] These effects arise from multiple mechanisms, including disruption of host cellular machinery (e.g., inhibition of DNA, RNA, or protein synthesis), alterations in ion balance and secondary messenger pathways, and structural remodeling of the cytoskeleton or plasma membrane by viral proteins.^[3] In some cases, CPE may also stem from the host's innate immune response, such as interferon-mediated pathways that amplify cellular damage.^[4] While traditionally viewed as inevitably leading to cell death, recent insights reveal that CPE is not always terminal; certain infected cells can survive by clearing the virus through non-cytolytic mechanisms, contributing to tissue repair and modulating immune responses.^[2] In virology, observing CPE remains a fundamental diagnostic tool for virus isolation and identification in clinical and research settings, often visualized under light microscopy in monolayer cell cultures.^[1] This phenomenon underscores the interplay between viral replication strategies and host defenses, influencing the pathogenesis of diseases ranging from common respiratory infections to severe conditions like HIV-associated cytopathology.^[5]

Fundamentals

Definition

The cytopathic effect (CPE) refers to the visible morphological and functional alterations in host cells induced by viral replication, including changes such as cell rounding, lysis, or fusion into syncytia.^[1] These effects manifest as degenerative changes observable under light microscopy, serving as a direct phenotypic marker of viral infection.^[6] CPE is particularly evident in cultured cells and is often virus-specific, varying based on the infecting virus, host cell type, and viral load.^[7] Key characteristics of CPE include its time-dependent progression during the viral replication cycle and its role in facilitating viral dissemination, such as through cell lysis or membrane alterations.^[1] Unlike non-cytopathic viruses (e.g., some retroviruses) that produce minimal observable changes, cytopathic viruses like herpes simplex or adenovirus trigger pronounced effects within days of infection.^[7] These alterations can include the formation of inclusion bodies or multinucleated giant cells, aiding in viral identification without advanced molecular techniques.^[6] While CPE often culminates in cell death through mechanisms such as apoptosis or necrosis, recent insights indicate it is not always terminal, with some infected cells surviving by clearing the virus through non-cytolytic pathways.^[2] It is distinct from but can overlap with processes like apoptosis (programmed cell death) or necrosis (uncontrolled lysis), as it specifically denotes virus-driven structural changes rather than the intrinsic cellular death pathways.^[8] For instance, in reovirus infections, CPE involves apoptotic mechanisms leading to morphological rounding and detachment.^[1] A typical progression begins with initial cell enlargement and granularity, followed by rounding, detachment from the substrate, and eventual lysis or fusion.^[7]

Historical Context

The recognition of cytopathic effects (CPE) began with early investigations into viral diseases, where observable cellular damage was first linked to a viral agent. In 1898, Dutch microbiologist Martinus Beijerinck studied tobacco mosaic disease in plants, observing characteristic mosaic patterns of chlorosis and necrosis in infected leaf cells and attributing them to a filterable, self-replicating contagium vivum fluidum rather than a bacterial product. This marked the initial connection between viral presence and specific cellular pathology, though the term "cytopathic effect" emerged later with animal cell studies.^[9] Advancements in the 1930s were crucial for enabling consistent observation of CPE. Pioneers like George O. Gey developed the roller drum technique in 1933, which allowed for the large-scale cultivation of mammalian tissues in rotating cylinders, improving nutrient distribution and cell viability in vitro. This innovation, building on earlier methods such as the 1928 Maitland technique using minced tissue suspensions, overcame prior challenges in maintaining viable cell monolayers and facilitated the controlled study of viral impacts on cultured cells.^[10]^[11] A pivotal milestone occurred in the 1950s with the work of John F. Enders, Thomas H. Weller, and Frederick C. Robbins, who in 1949 successfully propagated poliovirus in cultures of human embryonic tissues, including non-nervous cells like skin and intestine. They observed rapid CPE, such as cell rounding and lysis, confirming viral replication independent of the central nervous system and enabling large-scale virus production for the Salk polio vaccine. Their breakthrough, awarded the 1954 Nobel Prize in Physiology or Medicine, standardized CPE as a diagnostic and research tool in virology.^[12] Early research faced limitations, including confusion between viral CPE and damage from bacterial toxins or contaminants. For instance, Dmitri Ivanovsky's 1892 filtration experiments on tobacco mosaic virus suggested a bacterial toxin as the cause, but this view was soon challenged by Beijerinck's proposal of a viral agent. However, early 20th-century cell culture studies for animal viruses often suffered from bacterial contamination, which was effectively eliminated in the 1940s with antibiotics and refined techniques, confirming the unique, virus-specific nature of observed cellular changes. By mid-century, these refinements had evolved CPE from sporadic empirical notes into a reliable, quantitative virological assay.^[13]^[11]^[14]

Mechanisms

Direct Viral Effects

Viruses initiate cytopathic effects through direct interference with host cell processes during their replication cycle, beginning with viral entry via receptor-mediated endocytosis or fusion, which disrupts plasma membrane integrity. Once inside, viruses hijack host cellular machinery, including ribosomes and nucleotide pools, to prioritize viral genome replication and protein synthesis, leading to depletion of essential cellular resources such as ATP and amino acids. This resource diversion impairs normal host metabolism and triggers progressive cellular dysfunction, often culminating in membrane disruption as viral assembly sites form on intracellular membranes like the endoplasmic reticulum (ER) and Golgi apparatus.^[1] A key direct mechanism involves the accumulation of viral proteins that overload host organelles, inducing ER stress through misfolded protein buildup and glycoprotein overload in the ER lumen. For instance, many enveloped viruses, such as coronaviruses and flaviviruses, produce high levels of envelope glycoproteins that activate the unfolded protein response (UPR), causing ER dilation and calcium dysregulation, which exacerbate cellular damage independent of immune involvement. Additionally, viral enzymes, particularly proteases, directly degrade host structural components; picornavirus proteases like those in enteroviruses cleave cytoskeletal elements such as actin and intermediate filaments, destabilizing cell architecture and facilitating viral spread while promoting lysis.^[15] Specific examples illustrate these processes in RNA and DNA viruses. In poliovirus infection, the 2A protease cleaves eukaryotic initiation factor 4G (eIF4G), selectively inhibiting cap-dependent host translation while allowing internal ribosome entry site (IRES)-driven viral protein synthesis, which rapidly depletes host resources and leads to cell lysis within hours. Similarly, herpes simplex virus type 1 (HSV-1) replication in the nucleus forms replication compartments that expand and marginalize host chromatin, causing nuclear lamina disruption via viral proteins UL31 and UL34, resulting in nuclear enlargement and architectural collapse. The severity of these cytopathic effects correlates with viral replication rates; high-titer infections (e.g., multiplicity of infection >1) accelerate cell death, often observable within 24-48 hours post-infection, as seen in rapid lytic cycles of viruses like Sindbis virus in neural progenitors.^[16]^[17]^[18]

Indirect Host Effects

Indirect host effects in cytopathic effect (CPE) arise from the activation of the host's antiviral immune pathways, which can amplify cellular damage beyond direct viral replication. The interferon (IFN) response, a cornerstone of innate immunity, triggers the production of type I IFNs that induce hundreds of IFN-stimulated genes (ISGs) to restrict viral spread, but excessive IFN signaling can lead to a cytokine storm—a hyperinflammatory state characterized by elevated levels of pro-inflammatory cytokines such as TNF-α, IL-6, and IL-1β—resulting in bystander cell death through apoptosis or pyroptosis in uninfected neighboring cells. This process contributes to tissue pathology by promoting widespread inflammation and cellular demise, as seen in various viral infections where host-derived signals exacerbate CPE.^[19]^[20] Immune cell infiltration further intensifies indirect damage, with T-cells and macrophages migrating to infected sites and releasing mediators that induce inflammation-driven apoptosis in host cells. Activated CD8+ T-cells express Fas ligand (FasL), which binds to Fas receptors on target cells, activating caspase cascades that culminate in programmed cell death, while TNF-α secreted by immune cells similarly engages TNF receptors to trigger extrinsic apoptosis pathways, often independent of viral infection in the dying cells. This immune-mediated killing helps control viral dissemination but can cause collateral damage to healthy tissue, amplifying the overall cytopathic outcome.^[21]^[22] A prominent example is HIV-1, where CPE primarily manifests through immune activation rather than direct lysis of infected cells; chronic immune stimulation leads to bystander apoptosis of uninfected CD4+ T-cells via gp120-mediated upregulation of FasL and TNF-α, as well as pyroptosis triggered by abortive infection and inflammasome activation, contributing to progressive CD4+ T-cell depletion. Similarly, in influenza A virus infections, CPE is amplified by macrophage-released cytokines; infected alveolar macrophages produce excessive TNF-α and IL-1β, which exacerbate lung epithelial damage and inflammation, leading to heightened bystander cell death and severe respiratory pathology.^[23]^[24]^[25]^[26] These indirect effects are modulated by host genetics and viral evasion strategies. Variants in IFN receptors, such as homozygous mutations in IFNAR2 (e.g., c.A311del), impair type I IFN signaling and increase susceptibility to severe viral disease by reducing antiviral gene expression, thereby altering the balance between protective immunity and pathological inflammation. Viruses like flaviviruses employ non-structural (NS) proteins for immune evasion; for instance, dengue virus NS1 inhibits complement activation and IFN-β production, dampening host responses but paradoxically allowing unchecked inflammation that indirectly enhances CPE through endothelial dysfunction and apoptosis.^[27]^[28]

Morphological Types

Total Destruction

Total destruction is the most severe manifestation of cytopathic effect, involving rapid and widespread morphological alterations in infected host cells, including cell rounding, shrinkage with nuclear pyknosis, detachment from the substrate, and ultimate lysis, which collectively result in the complete clearing of the cell monolayer in culture.^[29] This uniform process leads to the total loss of the adherent cell layer, appearing as a clear, empty expanse under light microscopy at magnifications such as 200x.^[1] The underlying mechanism ties directly to the virus's replicative strategy, where a high burst size—typically producing 10^3 to 10^4 progeny virions per infected cell—overwhelms cellular integrity, culminating in plasma membrane rupture and lysis to facilitate viral release.^[30] In picornaviruses, this is exacerbated by viral proteins that inhibit host translation and transcription while increasing membrane permeability and ionic imbalance, accelerating cell death.^[4] Viruses exemplifying this CPE include enteroviruses such as coxsackieviruses and polioviruses within the picornavirus family, known for their cytolytic nature.^[29]^[1] The progression is swift, with initial signs of rounding visible 12-24 hours post-infection at high multiplicities, and full monolayer destruction achieved within 48 hours.^[29] Under microscopic observation, the distinct "clearing" of the cell sheet serves as a key visual endpoint in plaque assays for quantifying these viruses.^[1]

Subtotal Destruction

Subtotal destruction refers to a cytopathic effect characterized by the partial detachment and death of cells in a monolayer culture, leaving clusters of surviving cells amid areas of incomplete clearing, which progresses more slowly than complete monolayer lysis. This form of damage arises from direct viral effects on cellular integrity, such as membrane disruption, but does not lead to uniform cell death across the culture.^[1] Viruses associated with subtotal destruction include adenoviruses and certain herpesviruses, which often establish persistent infections without inducing full cell lysis in all infected hosts.^[31] For instance, adenovirus type 1 causes a slowly progressing lytic infection in cervical epithelial cultures, with ongoing viral production and partial cell loss allowing for viral persistence.^[32] Similarly, herpes simplex virus can maintain cyclic persistent infections in vitro, featuring localized areas of cell destruction interspersed with surviving cells that support intermittent viral replication. Visually, subtotal destruction manifests as patchy cell loss under microscopy, with affected cells exhibiting pyknosis—nuclear shrinkage and condensation—while unaffected or partially damaged cells remain attached and clustered, observable at 200x magnification without complete monolayer detachment. This uneven pattern contrasts with more aggressive total destruction by highlighting regions of viable cells amid degeneration. Biologically, this cytopathic effect reflects a delicate balance between viral replication demands and host cell survival mechanisms, such as autophagy, which enable partial recovery and viral containment in surviving cells during persistent infections.^[2] In adenoviruses and herpesviruses, this equilibrium supports long-term viral maintenance without immediate host cell elimination, facilitating chronic infection states.^[33]

Focal Degeneration

Focal degeneration is a type of cytopathic effect (CPE) characterized by discrete, localized areas of infected and degenerated cells within an otherwise intact monolayer of healthy cells in tissue culture. This pattern arises from viruses that propagate primarily through direct cell-to-cell contact, leading to the formation of small, expanding foci of damage rather than diffuse destruction across the culture. These foci typically manifest as early indicators of infection, appearing as rounded, swollen, or cleared spots amid the cell sheet, and can progressively enlarge if the infection continues unchecked.^[34] This form of CPE is commonly associated with certain herpesviruses, including cytomegalovirus (CMV) and varicella-zoster virus (VZV), which exhibit limited extracellular spread and instead rely on intercellular transmission. For CMV, the foci often present as clusters of enlarged, flat cells with a distinctive "owl's eye" appearance due to intranuclear inclusions under microscopic observation in human fibroblast cultures.^[35] Similarly, VZV infection produces a multifocal CPE, typically emerging 2 to 3 days post-infection in susceptible cell lines such as human melanoma or neuronal cells, with visible spots of cellular rounding and degeneration.^[36]^[37] The localized nature of focal degeneration makes it particularly valuable for virological assays, such as focus-forming assays, which quantify viral infectivity by counting the number of discrete foci formed after infection of a cell monolayer, often enhanced by immunostaining for precise detection. These assays are especially useful for titrating viruses like CMV, where traditional plaque assays may be less efficient due to the focal spread pattern. In some instances, focal degeneration may coincide with related changes like syncytium formation in herpesvirus infections.^[38]^[39]

Swelling and Clumping

Swelling and clumping is a form of cytopathic effect characterized by cytoplasmic ballooning, where infected host cells undergo significant enlargement, followed by their aggregation into tight clusters; this morphological alteration often serves as an early, potentially reversible indicator of viral replication that can precede more severe cellular damage if the infection persists.^[40] Visually, affected cells appear enlarged and refractile under phase-contrast microscopy, forming distinctive grape-like clumps due to reduced intercellular adhesion while remaining attached to the substrate initially.^[40] This type of cytopathic effect is classically observed with adenoviruses, though similar changes can occur with other viruses capable of inducing comparable cellular stress.^[40] If the viral load is controlled, such as through host immune clearance or antiviral intervention, the swelling and clumping may resolve, restoring cellular integrity without progression to irreversible degeneration.^[2] The biological basis involves disruptions in ion homeostasis and cytoskeletal integrity, often triggered by viral manipulation of host metabolic pathways. For instance, adenovirus infection promotes enhanced glycolysis, leading to lactate accumulation and intracellular acidosis, which drives calcium ion influx and subsequent osmotic water entry, causing cytoplasmic swelling.^[41] Concurrently, viral proteins interfere with actin filaments and other cytoskeletal components, impairing cell shape maintenance and adhesion molecules, thereby facilitating the non-fusogenic clumping of enlarged cells.^[42] These osmotic shifts, exacerbated by viral metabolites like lactate, underscore the role of membrane permeability alterations in amplifying the cytopathic response.^[41]

Foamy Degeneration

Foamy degeneration, also known as vacuolization, is a morphological cytopathic effect characterized by the formation of numerous small or several large cytoplasmic vacuoles within infected host cells, resulting in a distinctive foamy or bubbly texture to the cytoplasm.^[43] These vacuoles arise from disruptions in cellular homeostasis, often involving alterations in membrane trafficking, lysosomal swelling, or accumulation of lipids and cellular debris within membrane-bound compartments.^[44] In cytopathic strains of bovine viral diarrhea virus (BVDV), for instance, extensive cytoplasmic vacuolization represents the earliest observable change following infection, preceding more severe cellular damage and linked to endoplasmic reticulum stress responses.^[45] Similarly, in human immunodeficiency virus (HIV) infection, vacuolization contributes to ballooning degeneration, potentially driven by ion imbalances and excessive viral particle binding that overwhelms cellular membrane dynamics.^[5] This cytopathic effect is prominently associated with certain retroviruses and pestiviruses, including HIV and cytopathic biotypes of BVDV, which induce vacuole formation without immediate nuclear involvement.^[43] Other viruses, such as some paramyxoviruses and flaviviruses, can also elicit similar vacuolization, though BVDV exemplifies the process in veterinary contexts by producing large cytoplasmic vacuoles that confer a foamy appearance to infected bovine cells.^[46] In HIV-infected cells, particularly T lymphocytes and macrophages, the vacuoles often stem from membrane alterations that impair endosomal-lysosomal function, leading to lipid-laden compartments.^[47] Under phase-contrast microscopy, the bubbly cytoplasm of vacuolized cells appears as refractile, clear spaces scattered throughout the perinuclear and peripheral regions, with infected cells typically remaining adherent to the culture substrate for extended periods compared to lytic cytopathic effects.^[43] Staining techniques, such as Giemsa, enhance visualization by outlining the vacuoles as unstained voids against the basophilic cellular background, as seen in BVDV-infected bovine fetal spleen cells where arrows highlight prominent vacuolar structures at 400× magnification.^[43] This adherent nature allows for prolonged observation of the progressive vacuolization before eventual cell rounding or detachment. The pathology of foamy degeneration can resemble non-viral degenerative processes, such as ischemic cellular injury, due to shared features of cytoplasmic swelling and vacuole formation from metabolic stress.^[48] Progression to cell death varies; in BVDV infections, vacuolization may evolve into apoptosis via unfolded protein response activation, while in HIV, it often leads to necrosis-like lysis, though some cells persist with chronic vacuolization without rapid demise.^[45]^[5]

Syncytium Formation

Syncytium formation is a distinctive cytopathic effect characterized by the fusion of adjacent infected cells, mediated by viral fusion proteins expressed on the host cell membrane, resulting in the creation of large, multinucleated giant cells known as syncytia or polykaryocytes. These syncytia typically contain 10 to several hundred nuclei within a shared cytoplasm, preserving overall cell mass while facilitating direct intercellular viral propagation.^[49]^[50] This process is prominently associated with infections by paramyxoviruses, such as measles virus and respiratory syncytial virus (RSV), as well as herpesviruses including herpes simplex virus (HSV) and varicella-zoster virus (VZV). In paramyxoviruses, syncytium formation requires the coordinated action of the hemagglutinin-neuraminidase (HN) or hemagglutinin (H) glycoprotein, which binds to host cell receptors like SLAM (CD150) or nectin-4, and the fusion (F) glycoprotein, which drives membrane merger through conformational changes. Herpesviruses employ a complex of glycoproteins, including glycoprotein B (gB) as the primary fusogen and the gH/gL heterodimer, often triggered by receptor interactions such as those with nectin-1 or HVEM.^[49]^[51]^[52] Visually, syncytia appear as irregularly shaped, enlarged cells with densely packed nuclei and a granular, shared cytoplasm, observable under light microscopy in infected cell cultures without the need for staining. The fusion occurs at the plasma membrane in a pH-independent manner for these viruses, bypassing endocytosis and enabling rapid cell-to-cell contact without extracellular virion release. This mechanism enhances viral dissemination within tissues, such as the respiratory epithelium for RSV or lymph nodes for measles, by amplifying infection foci and evading humoral immune responses.^[49]^[53]^[51]

Inclusion Bodies

Inclusion bodies are intracellular accumulations of viral particles, proteins, or cellular debris that form as a visible manifestation of cytopathic effect in virus-infected cells. These structures typically appear as aggregates that alter the staining properties of the affected cells and are not present in viable, uninfected cells. They can be eosinophilic, staining pink with eosin dye, or basophilic, staining blue with hematoxylin, and range in size from 1 to 20 μm depending on the virus and stage of infection.^[1] Inclusion bodies are located either in the nucleus or cytoplasm, serving as sites for viral replication or assembly. Nuclear inclusions, such as Cowdry type A bodies, are characteristic of certain DNA viruses like herpes simplex virus (HSV), appearing as eosinophilic masses surrounded by a clear halo. Cytoplasmic inclusions include Guarnieri bodies associated with poxviruses, which are eosinophilic aggregates in epithelial cells, and Negri bodies in rabies virus infections, which are round to oval eosinophilic structures measuring 2-10 μm. These inclusions are diagnostic hallmarks for specific viral infections, such as Negri bodies confirming rabies.^[54]^[1]^[55] Detection of inclusion bodies relies on histological staining, particularly hematoxylin and eosin (H&E), where they appear as distinct areas of altered coloration against the cellular background. They are aggregates of virions, viral proteins, or host-derived debris resulting from direct viral protein accumulation during replication. Pathologically, these bodies interfere with host cell transcription and translation by sequestering cellular machinery or disrupting nuclear and cytoplasmic functions, contributing to cell dysfunction and eventual lysis. While eosinophilic inclusions are more common in DNA virus infections, basophilic ones occur in some RNA virus cases, reflecting differences in viral genome and protein composition.^[54]^[1]

Diagnostic Applications

In Vitro Methods

In vitro methods for detecting cytopathic effect (CPE) involve cultivating viruses in susceptible cell monolayers to observe virus-induced cellular damage under controlled laboratory conditions. Standard protocols typically begin with seeding permissive cell lines, such as Vero cells, into multi-well plates to form confluent monolayers, followed by inoculation with serial dilutions of the virus stock.^[56] The inoculum, often 100 µL per well in 96-well plates, is adsorbed onto the cells for 1-2 hours at 37°C, after which maintenance medium is added, and cultures are incubated at 37°C in 5% CO₂ for 3-7 days, with daily microscopic monitoring for morphological changes indicative of CPE, such as cell rounding or lysis.^[56]^[57] Key assay types include the tissue culture infectious dose 50 (TCID₅₀) assay, which quantifies viral infectivity by determining the dilution at which 50% of replicate wells exhibit CPE, calculated using methods like Reed-Muench or Spearman-Kärber based on endpoint observations.^[57] Another common approach is the cytopathic effect inhibition (CPEI) assay, used for antiviral screening, where test compounds are pre-incubated with cells before viral challenge; inhibition is measured by reduced CPE compared to virus-only controls, often via viability dyes like neutral red or luminescent assays such as CellTiter-Glo.^[58]^[56] For respiratory viruses like human respiratory syncytial virus (hRSV), permissive lines such as HEp-2 cells are preferred due to their susceptibility and ability to support robust viral replication, enabling clear CPE visualization within 4-6 days.^[59] These methods offer advantages over animal models, including faster turnaround times (days versus weeks), lower costs, and ethical benefits by reducing animal use while providing reproducible results for diagnostic and screening purposes.^[60]^[61] Essential controls include mock-infected cells treated with medium alone to differentiate viral CPE from non-specific toxicity, alongside positive controls like known antivirals (e.g., remdesivir) and vehicle controls (e.g., DMSO).^[56] Quantification beyond visual inspection often employs dye uptake assays, such as neutral red for viable cell assessment via absorbance at 540 nm, ensuring objective measurement of CPE extent across replicates.^[56]

Observation Techniques

Cytopathic effects (CPE) in infected cells are primarily observed using microscopy techniques that allow visualization of morphological changes without or with minimal sample preparation. Light microscopy, particularly phase-contrast microscopy, enables the real-time monitoring of live cells exhibiting CPE, such as cell rounding, detachment, or lysis, in cell culture systems. This method is widely used due to its non-invasive nature and ability to detect early morphological alterations in virus-infected monolayers. For finer details, fluorescence microscopy combined with vital dyes can highlight specific cellular changes, though it requires careful selection to avoid confounding cell toxicity. Transmission electron microscopy (TEM) provides ultrastructural insights into CPE, revealing details like membrane disruptions, organelle damage, and virion assembly or release at the plasma membrane. In poliovirus-infected cells, TEM has historically visualized cytoplasmic vacuolization and viral particle budding as key CPE features. Similarly, in SARS-CoV-2 infections, TEM demonstrates double-membrane vesicles and virion egress, correlating these with plaque-like cytopathic lesions in airway epithelia. Scanning electron microscopy (SEM) complements TEM by offering surface topography views of infected cells, showing protrusions or blebbing indicative of apoptosis during CPE. Staining techniques enhance the specificity of CPE observation by targeting viral components or cellular responses. Immunofluorescence (IF) staining detects viral antigens in CPE-affected cells, using antibodies conjugated to fluorophores to localize proteins like nucleocapsids or envelope glycoproteins within altered cellular structures. For instance, IF assays confirm SARS-CoV-2 presence in cultured cells showing syncytia formation, providing both qualitative and semi-quantitative data on infection extent. Histochemical stains, such as those for inclusion bodies, further characterize CPE; the Feulgen reaction specifically stains DNA-rich intranuclear inclusions, aiding differentiation from non-viral changes, though it is less commonly used today due to advanced molecular alternatives. Advanced imaging modalities track CPE dynamics over time. Time-lapse microscopy captures the progression of CPE, from initial cell entry to full degeneration, using automated phase-contrast or holographic setups to monitor infected populations non-destructively. In norovirus studies, time-lapse imaging has quantified plaque expansion and cell death kinetics at 30-minute intervals, revealing temporal patterns of cytolysis. Flow cytometry quantifies CPE-induced cell death modes by distinguishing apoptotic (annexin V-positive) from necrotic (propidium iodide-positive) populations in virus-infected samples. For respiratory syncytial virus, flow cytometry has shown dose-dependent apoptosis in epithelial cells, linking it to CPE severity without relying on morphological assessment alone. In vivo observation of CPE-like changes extends to tissue samples from animal models or patients, primarily through biopsy histology. Hematoxylin and eosin (H&E) staining of liver biopsies in hepatitis B virus (HBV)-infected humanized mice reveals ground-glass hepatocytes and inflammatory infiltrates mimicking in vitro CPE. In COVID-19 patient autopsies and animal models like hamsters, histological analysis identifies alveolar damage and endothelial CPE analogs, confirmed by immunohistochemistry for viral antigens. These methods bridge lab observations to pathological contexts, though they require ethical considerations and correlative in vitro validation.

Significance

In Viral Pathogenesis

The cytopathic effect (CPE) plays a central role in viral pathogenesis by directly damaging infected cells, leading to tissue dysfunction and clinical symptoms. In respiratory syncytial virus (RSV) infections, CPE on the respiratory epithelium causes sloughing of ciliated cells and inflammation, resulting in airway obstruction and the characteristic wheezing and dyspnea of bronchiolitis in infants.^[62] Similarly, in rabies virus infection, neuronal CPE manifests as apoptosis and cytopathic changes in motor neurons, contributing to the neurological dysfunction and fatal encephalitis observed in affected individuals.^[63] Tissue-specific CPE further drives localized pathology in various viral infections. Varicella-zoster virus (VZV) induces multinucleated giant cell formation through cell fusion in epidermal keratinocytes, leading to intraepidermal vesicle development and the vesicular rash characteristic of chickenpox.^[64] Additional examples highlight CPE's impact on vascular and pulmonary tissues. Ebola virus infection of endothelial cells, though not primarily through overt cytolysis, induces functional alterations and viral replication that exacerbate coagulopathy and vascular permeability, culminating in the hemorrhagic manifestations of Ebola virus disease.^[65] For SARS-CoV-2, CPE in alveolar epithelial cells results in diffuse alveolar damage, including syncytia formation and inflammatory cell infiltration, which drives the hypoxemia and acute respiratory distress syndrome (ARDS) seen in severe COVID-19 cases.^[66] The severity of CPE often correlates with viremia levels, as higher viral loads facilitate widespread cellular infection and damage, influencing disease progression.^[67] In chronic infections, such as cytomegalovirus (CMV) in immunocompromised hosts, persistent CPE leads to ongoing tissue injury, including retinitis, pneumonitis, and gastrointestinal ulceration, due to reactivation and unchecked viral replication in the absence of effective immunity.^[68]

In Virology Research

In virology research, the cytopathic effect (CPE) serves as a foundational tool for virus isolation and discovery, particularly in surveillance programs where clinical samples are inoculated into susceptible cell lines to observe morphological changes indicative of viral replication. For instance, the initial isolation of the novel coronavirus HCoV-EMC from a patient with pneumonia in 2012 relied on Vero cell cultures, where the virus induced characteristic CPE including cell rounding, detachment, and syncytium formation, enabling its identification and propagation. Similarly, SARS-CoV-2 was first isolated from Korean and U.S. patients in early 2020 using Vero cells, with confluent CPE observed after 3 days of culture, confirming infectivity and facilitating further genomic characterization. These examples highlight CPE's role as a primary screen in detecting emerging cytolytic viruses before molecular confirmation, though it requires permissive cell lines and can take days to manifest. CPE assays are integral to high-throughput antiviral drug screening, quantifying the protective effect of compounds against virus-induced cell damage. In such assays, candidate drugs like remdesivir are tested for their ability to reduce CPE in infected cell monolayers, often measured by cell viability metrics such as neutral red uptake or luminescence to determine half-maximal effective concentrations (EC₅₀). For SARS-CoV-2, remdesivir demonstrated potent inhibition of CPE in Vero E6 cells with an EC₅₀ of approximately 0.77 μM, supporting its rapid advancement to clinical trials through quantitative high-throughput screens involving thousands of repurposed compounds. These assays prioritize infectivity over mere viral presence, providing a direct readout of antiviral efficacy, though they are complemented by plaque reduction assays for precision. Genetic studies leverage CPE to dissect host-virus interactions, employing CRISPR-Cas9 knockouts to identify cellular factors modulating viral cytopathogenicity. Genome-wide CRISPR screens in cytopathic virus-susceptible cells, such as those infected with influenza or SARS-CoV-2, select for knockouts that rescue cells from CPE-induced death, revealing essential host genes like those in interferon signaling pathways (e.g., IRF3 and IRF7) that restrict viral spread. For example, knockout of interferon regulatory factors in Vero cells enhanced viral yields while reducing CPE, elucidating innate immune contributions to pathogenesis. Reporter viruses further enhance these investigations by linking fluorescence to CPE-linked infection; recombinant influenza A viruses expressing enhanced green fluorescent protein (EGFP) or NanoLuc luciferase allow real-time tracking of replication dynamics, where fluorescence intensity correlates with CPE progression in live cells, bypassing endpoint staining. Despite its utility, CPE-based methods face limitations, including subjectivity in visual scoring, delayed detection for non-cytopathic viruses, and labor-intensive protocols, prompting a shift toward molecular alternatives like quantitative PCR (qPCR) for faster, more sensitive viral RNA quantification without relying on cellular damage. However, CPE remains the gold standard for assessing infectivity of cytolytic viruses, as it directly measures viable replication in host cells, unlike qPCR which detects nucleic acids irrespective of infectivity. Advances in the 2020s, particularly AI-driven imaging, have addressed these challenges by enabling automated, label-free CPE scoring; convolutional neural networks trained on brightfield microscopy images differentiate subtle pre-CPE morphological changes in influenza- or SARS-CoV-2-infected cells with over 95% accuracy, accelerating high-throughput antiviral assays and reducing manual bias. Tools like the AI Recognition of Viral CPE (AIRVIC) system classify virus-induced effects in diverse samples, including saliva, enhancing scalability in research pipelines.

References

[1]
Effects on Cells - Medical Microbiology - NCBI Bookshelf - NIH
A particularly striking cytopathic effect of some viral infections is the formation of syncytia, or polykaryocytes, which are large cytoplasmic masses that ...
[2]
Revisiting the concept of a cytopathic viral infection - PMC
Jul 20, 2017 · Cytopathic viral infections typically cause cell death, but some cells can survive and eliminate the virus, forming "survivor" cells.
[3]
Cytopathic Effect - an overview | ScienceDirect Topics
Cytopathic effect (CPE) is the morphological modification or death of host cells caused by viral replication, often shutting off cellular macromolecule ...
[4]
Cytopathic effects: virus-modulated manifestations of innate immunity?
It underlies viral disease-causing capacities. Various pathological cell alterations triggered by eukaryotic viruses are referred to as the cytopathic effect ( ...
[5]
Cytopathic Mechanisms of HIV-1 - Virology Journal - BioMed Central
Oct 18, 2007 · These cytopathic effects – syncytial cell formation, balloon degeneration and cell death – are all observed in the absence of reverse ...
[6]
The Virus-Induced Cytopathic Effect - PubMed
The cytopathic effect comprises the set of cellular alterations produced by a viral infection. It is of great relevance since it constitutes a direct marker ...Missing: definition virology
[7]
Cytopathogenic Effect - an overview | ScienceDirect Topics
Cytopathogenic effects (CPE) refer to the degenerative changes that cells undergo when infected with a virus, which can be observed as multinucleated giant ...
[8]
Apoptosis and the Cytopathic Effects of Reovirus - SpringerLink
The description and definition of apoptosis as a mechanism of cell death distinct from necrosis has provided a framework for elucidating the roles of these ...Chapter Pdf · Similar Content Being Viewed... · Author Information
[9]
Beijerinck's work on tobacco mosaic virus: historical context ... - NIH
Beijerinck's entirely new concept, launched in 1898, of a filterable contagium vivum fluidum which multiplied in close association with the host's metabolism.Missing: cytopathic effect discovery
[10]
George Otto Gey (1899-1970) | Embryo Project Encyclopedia
Mar 21, 2022 · Gey developed new techniques for in vitro, or laboratory-based, maintenance of organs and hormonal tissue, created new methods for cell ...
[11]
Historical Overview - PMC - PubMed Central
The discovery of foot-and-mouth disease virus by Friedrich Loeffler and Paul Frosch in Greifswald in 1898 was the first evidence of an animal pathogenic virus.
[12]
Cultivation of the Lansing Strain of Poliomyelitis Virus in ... - Science
Cultivation of the Lansing Strain of Poliomyelitis Virus in Cultures of Various Human Embryonic Tissues. John F. Enders, Thomas H. Weller, and Frederick ...
[13]
Introduction: A Short History of Virology - PMC - NIH
Pasteur also successfully isolated organisms involved in sepsis, which results in septic shock due to the growth of bacteria and resulting toxins in the blood.
[14]
The expanding roles of endoplasmic reticulum stress in virus ...
Based on the fact that some viral proteins can trigger ER stress through interacting with ER chaperones, the Togaviridae family virus Rubella virus (RV) ...
[15]
The Multifaceted Poliovirus 2A Protease: Regulation of Gene ...
Interestingly, individual expression of the viral proteases 2Apro and 3Cpro leads to the induction of most of these cytopathic effects, supporting the idea ...
[16]
Identification and Functional Evaluation of Cellular and Viral Factors ...
Herpes simplex virus 1 (HSV-1) replicates in the nucleus of host cells and radically alters nuclear architecture as part of its replication process.Cells And Viruses · Antibodies And... · Discussion
[17]
Cellular responses to Sindbis virus infection of neural progenitors ...
Oct 24, 2014 · Here, we show that hNPCs are highly susceptible to SINV infection with a cytopathic effect (CPE) starting at 24 hours post infection (h.p.i).
[18]
Off balance: Interferons in COVID-19 lung infections - PMC - NIH
Oct 19, 2021 · The imbalance of Type I, Type II and Type III Interferons during a viral infection contribute to the clinical outcome, possibly together with other cytokines.
[19]
Harnessing the immune system to overcome cytokine storm ... - NIH
Oct 15, 2020 · The direct virus-induced cytopathic effect on type two pneumocytes via ACE2 receptors activates the innate immune system, resulting in a large ...
[20]
Mechanisms of Human Immunodeficiency Virus-Associated ...
The HIV-1 cytopathic effect was once considered to be the primary mechanism of CD4 T cell destruction. Subsequent studies showed that CD4 T cell depletion and ...
[21]
Host and Viral Factors in HIV-Mediated Bystander Apoptosis - PMC
Abstract. Human immunodeficiency virus (HIV) infections lead to a progressive loss of CD4 T cells primarily via the process of apoptosis.2. Viral Factors · 2.7. Effects Of Hiv Env On... · 4. Immune Activation
[22]
HIV-1 Induced Bystander Apoptosis - PMC - PubMed Central
Apoptosis of uninfected bystander cells is a key element of HIV pathogenesis and believed to be the driving force behind the selective depletion of CD4+ T cells ...
[23]
Abortive HIV Infection Mediates CD4 T-Cell Depletion and ...
We now show that the death of these “bystander” cells involves abortive HIV infection. Inhibitors blocking HIV entry or early steps of reverse transcription ...Figure 2. Cd4 T-Cell... · Hiv Gp41-Mediated Fusion Is... · Non-Permissive Cd4 T Cells...
[24]
Severe influenza infection is associated with inflammatory ...
IAV-infected macrophage death has been related to overproduction of local and systemic cytokines/chemokines, cellular infiltration, extracellular matrix ...
[25]
Pathogenicity and virulence of influenza - PMC - PubMed Central
Jun 20, 2023 · Severe influenza viral disease is caused by both direct viral cytopathic effects and exacerbated host immune response against high viral loads.
[26]
The role of host genetics in susceptibility to severe viral infections in ...
Sep 9, 2020 · Some studies showed that genetic mutations had a negative effect on interferon or cytokine secretions, which decreased in vitro in all cell ...
[27]
Flavivirus NS1 and Its Potential in Vaccine Development - PMC
Jun 9, 2021 · Flaviviruses cause invaginations in the ER membrane to form vesicles that are used for RNA replication involving the seven NS proteins that form ...
[28]
Protocols Cytopathic Effects of Viruses
Morphological changes in cells caused by viral infection are called cytopathic effects (CPE); the responsible virus is said to be cytopathogenic.Missing: definition | Show results with:definition
[29]
The life cycle of non-polio enteroviruses and how to target it - Nature
Apr 6, 2018 · Collectively, these changes induce dramatic changes in cellular morphology (that is, a cytopathic effect) and culminate in death and lysis of ...Missing: burst | Show results with:burst
[30]
Cytopathic effect (CPE) of Viruses: Types with Examples
This CPE is typical of most enteroviruses. Cytopathic effect HSV. Subtotal destruction. It consists of detachment (death) of some but not all of the cells in ...
[31]
Adenovirus Infection and Cytopathic Alterations of Human Cervical ...
In both types of cultures adenovirus type 1 resulted in a slowly progressing lytic infection with persistent production of infectious virus, viral antigens and ...
[32]
The UPR sensor IRE1α and the adenovirus E3-19K glycoprotein ...
Apr 24, 2020 · Here, we elucidate a mechanism supporting the persistence of human adenovirus (AdV), a virus that can kill immunosuppressed patients.
[33]
Diagnosis of Cytomegalovirus Infections - PMC - PubMed Central
In the standard tube cell culture technique, CMV exhibits a typical cytopathic effect (CPE) characterized by foci of flat, swollen cells where the CPE is ...Maternal And Fetal... · Congenital Cmv Infection · Cmv Infections In...
[34]
Varicella-zoster virus infection of human brain cells and ganglion ...
Two to three days after VZV infection of all cell cultures at a multiplicity of infection (MOI) of 0.1, a multifocal cytopathic effect (CPE) developed.Missing: focal | Show results with:focal
[35]
Varicella-Zoster Virus Infection of Neurons Derived from Neural ...
Mar 15, 2021 · A multifocal cytopathic effect (cpe), including the presence of virus-specific intranuclear inclusions, developed in all cell types after 2–3 ...Missing: focal | Show results with:focal
[36]
Application of a focus formation assay for detection and titration of ...
Table 1. Comparison of virus titer determined by plaque assay technique and focus forming assay (α ≤ 0.05) ... virus before discernable CPE is observed.
[37]
DUX4-stimulated genes define an antiviral defense program in ...
Sep 18, 2025 · HCMV infectious virus was quantified by focus-forming assay. Briefly, supernatants from HCMV replication experiments were serially diluted ...
[38]
https://pmc.ncbi.nlm.nih.gov/articles/PMC7119775/
[39]
Metabolome-Driven Regulation of Adenovirus-Induced Cell Death
Here, we review different types of adenovirus-induced cell death associated with changes in metabolic profiles of the infected cells.
[40]
Virus interactions with the actin cytoskeleton—what we know and do ...
Jan 31, 2022 · Here, we describe how the actin cytoskeleton is involved in the strategies used by different viruses for entry, assembly, and egress from the host cell.
[41]
[PDF] Cytopathic Effects of Viruses Protocols
Sep 29, 2007 · Note: it is important to view infected cells at a low MOI to determine if cytopathic effect is focal or generalized, at a high MOI all CPE ...Missing: first | Show results with:first
[42]
Cytoplasmic vacuolization responses to cytopathic bovine viral ...
Our results show that extensive cytoplasmic vacuolization is the earliest morphological change in response to cpBVDV infection in MDBK cells.Missing: foamy | Show results with:foamy
[43]
<i>Bovine viral diarrhea virus 2</i> Infection Activates the ... - J-Stage
The mechanism of cytopathogenicity of BVDV-2 is not well understood. We examined cytopathogenesis in MDBK cells resulting from BVDV-2 infections by microscopic ...
[44]
Detection of Cattle Infected with Bovine Viral Diarrhea Virus Using ...
The cytopathic biotype, BVDV-cp, causes char- acteristic formation of large vacuoles in the infected cell cytoplasm, giving it a “foamy” appearance, where- as ...Missing: vacuolization | Show results with:vacuolization
[45]
Cytopathic Mechanisms of HIV-1 - PMC - PubMed Central - NIH
Vacuolization was most prominent in cells with the highest numbers of bound virions. The authors hypothesize that the cell may be pumping excess ions into the ...Missing: foamy | Show results with:foamy
[46]
Myocyte degeneration and cell death in hibernating ... - PubMed
The aim of this study was to analyze the morphologic characteristics of myocyte degeneration leading to replacement fibrosis in hibernating myocardium.
[47]
https://pmc.ncbi.nlm.nih.gov/articles/PMC2174939/
[48]
https://pubmed.ncbi.nlm.nih.gov/8636539/
[49]
Measles Virus Fusion Protein: Structure, Function and Inhibition - PMC
Apr 21, 2016 · Recent research has shown that membrane fusion induced by the morbillivirus glycoproteins is the first critical step for viral entry and infection.
[50]
https://www.sciencedirect.com/science/article/pii/B9780122530555500074
[51]
Syncytium formation induced by viral infection - ViralZone
Syncytia result from the fusion of an infected cell with neighboring cells, leading to the formation of multinucleated, enlarged cells.Missing: cytopathic effect mechanisms
[52]
Special Studies - PMC - PubMed Central - NIH
Negri bodies: cytoplasmic round to oval or bullet-shaped eosinophilic inclusions ... Cowdry type B inclusions were described as smaller inclusions ...<|separator|>
[53]
11 Poxviruses - Basicmedical Key
Feb 19, 2017 · Eosinophilic inclusions, called Guarnieri bodies, can be seen in the cytoplasm. Multiple viral proteins, such as complement regulatory and ...
[54]
Cytopathic Effect Assay and Plaque Assay to Evaluate in vitro ... - NIH
Feb 5, 2022 · We provide the detailed protocol for the cytopathic effect (CPE) assay and the plaque assay for human coronaviruses 229E (HCoV-229E), HCoV-OC43, and HCoV-NL63,Missing: virology | Show results with:virology
[55]
On the Calculation of TCID50 for Quantitation of Virus Infectivity - NIH
May 26, 2020 · The median tissue culture infectious dose (TCID50) is defined as the dilution of a virus required to infect 50% of a given cell culture. Several ...
[56]
SARS-CoV-2 cytopathic effect (CPE) - NCBI
SARS-CoV-2 CPE is a loss of host cell viability caused by viral infection and replication. The assay measures compounds that reverse this effect.
[57]
A cell based high-throughput screening approach for the discovery ...
Jan 10, 2013 · A robust automated luminescent-based high throughput screen that measures the inhibition of hRSV-induced cytopathic effect in HEp-2 cells<|control11|><|separator|>
[58]
Role of Cell Culture for Virus Detection in the Age of Technology
This review describes advances in cell culture-based viral diagnostic products and techniques, including the use of newer cell culture formats, cryopreserved ...
[59]
Viral contamination in cell culture: analyzing the impact of Epstein ...
Cell culture techniques are increasingly favored over animal models due to rising costs, time constraints, and ethical concerns regarding animal use.
[60]
[Respiratory syncytial virus infection and asthma] - PubMed
The breathing difficulties that appeared in the initial outbreak of bronchiolitis is well explained by the cytopathic effect of the virus on the airways of ...<|separator|>
[61]
Viral Diseases of the Central Nervous System - PMC
Feb 12, 2015 · Although virus-induced cytopathology is undoubtedly contributing to the destruction of motor neurons, the role of immune-mediated pathology in ...
[62]
Detection of multinucleated giant cells in differentiated keratinocytes ...
Sep 17, 2020 · Herpes simplex virus (HSV) and varicella zoster virus (VZV) infections induce the formation of intraepidermal vesicles containing ...
[63]
Direct cytopathic effects of particular hepatitis B virus genotypes in ...
By using a humanized in vivo model, we show that different HBV genotypes and even particular mutations resulted in different virologic and histopathologic ...Missing: biopsy | Show results with:biopsy
[64]
Pathogenesis of Ebola hemorrhagic fever in primate models - PubMed
Ebola virus (EBOV) infection causes a severe and often fatal hemorrhagic disease in humans and nonhuman primates. Whether infection of endothelial cells is ...<|separator|>
[65]
COVID-19 and Sepsis - PubMed
Dec 17, 2021 · ... CoV-2 ... alveolar damage in lung tissue consistent with acute respiratory distress syndrome (ARDS). The presence of viral cytopathic-like changes ...Missing: effect | Show results with:effect
[66]
High Titers of Cytopathic Virus in Plasma of Patients with ...
Apr 4, 1991 · Primary, symptomatic HIV-1 infection is associated with high titers of cytopathic, replication-competent viral strains, and during such infection potential ...
[67]
Cytomegalovirus Infections - StatPearls - NCBI Bookshelf - NIH
May 11, 2025 · When patients are immunocompromised, eg, after solid organ and hematopoietic transplantation or on chemotherapy, CMV can reactivate, causing CMV ...