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Hereditary angioedema

Hereditary is a rare autosomal dominant characterized by recurrent episodes of severe, potentially life-threatening swelling () in the subcutaneous tissues, , and upper airway, resulting from dysregulation of the due to deficiency or dysfunction of the C1 esterase inhibitor (C1-INH) protein. The condition arises primarily from pathogenic variants in the SERPING1 gene, which encodes C1-INH, leading to uncontrolled activation of the kallikrein-kinin system and excessive production of , a potent vasodilator that increases and causes fluid leakage into tissues. HAE is classified into three main types: type 1, accounting for 80-85% of cases, features low levels of C1-INH due to reduced protein synthesis; type 2, comprising 15-20% of cases, involves normal or elevated C1-INH levels but with impaired function; and type 3, a rarer form with normal C1-INH levels often linked to mutations in the F12 gene encoding , which is more prevalent in females and exacerbated by . Epidemiologically, HAE affects approximately 1 in 50,000 individuals worldwide, with no significant racial or ethnic predisposition, though true may be underestimated due to underdiagnosis; symptoms typically onset in childhood or , with attacks occurring every 1-4 weeks and lasting 2-5 days, often triggered by , , infections, or hormonal changes. Clinically, the is nonpruritic, nonpitting, and lacks urticaria, distinguishing it from allergic reactions; while most episodes are self-limiting, laryngeal involvement in 1-3% of attacks can cause airway obstruction and requires urgent intervention.

Clinical Presentation

Signs and Symptoms

Hereditary angioedema (HAE) is characterized by recurrent episodes of non-pitting subcutaneous or submucosal edema that typically last 1 to 5 days and resolve spontaneously without . These swellings occur without accompanying urticaria, distinguishing HAE from allergic forms of . The most common sites of swelling include the , where painless, disfiguring affects the hands, feet, or limbs; the face, causing swelling around the eyes, , or cheeks; and the genitals, leading to discomfort and functional impairment. Abdominal attacks involve severe, colicky due to intestinal wall , often accompanied by , , and , which can mimic acute surgical emergencies such as . Laryngeal edema represents a particularly dangerous manifestation, resulting in swelling of the , , or upper airway that can cause hoarseness, , and life-threatening airway obstruction if untreated. Approximately 50% of patients experience at least one such episode over their lifetime. Some patients report prodromal symptoms preceding attacks, such as a serpentine, non-pruritic known as , which occurs in about one-third of cases, along with , muscle aches, or mood changes. Attack frequency varies widely among individuals, typically ranging from 10 to 30 per year in untreated patients, with onset typically in childhood or and potential worsening during .

Triggers and Complications

Hereditary angioedema (HAE) attacks can be precipitated by various identifiable factors, though approximately 60% occur without a clear trigger. Common precipitants include physical , such as minor injuries to the face or extremities, which is reported in up to 39% of initial episodes. Emotional or physical stress and anxiety frequently exacerbate attacks, as do infections, particularly ones. or dental procedures, including invasive interventions like or dental work, are well-documented triggers due to tissue manipulation. Hormonal fluctuations also play a significant role, especially in women; estrogen-containing oral contraceptives worsen symptoms in about 80% of affected individuals, while triggers attacks in 35% and in 14%. has variable effects, often increasing attack frequency in the first or third trimester due to rising levels. (ACE) inhibitors are contraindicated as they heighten attack risk and severity by further impairing degradation. Unlike allergic , HAE is mediated by rather than , so allergens and antihistamines do not trigger or alleviate attacks. Complications of HAE primarily arise from the location and severity of swelling episodes, which can last 2–5 days and significantly impair through recurrent and . Abdominal attacks, occurring in up to 80% of patients, often mimic and lead to intestinal causing obstruction, severe , nausea, , and ; these may result in , circulatory collapse in 4.4% of severe cases, and unnecessary surgeries due to misdiagnosis, with approximately 33% of patients undergoing procedures like . Laryngeal , though rare (1–3% of attacks), affects up to 50% of patients over their lifetime and poses a life-threatening risk of asphyxiation and airway obstruction, historically accounting for about 30% of HAE-related mortality in untreated cases. Rare associations include from prolonged during gastrointestinal episodes and secondary bacterial infections complicating unresolved skin swellings. Chronic frequent attacks contribute to ongoing morbidity, including from subcutaneous swelling and reduced daily functioning.

Pathogenesis

Genetics

Hereditary angioedema (HAE) types I and II follow an autosomal dominant inheritance pattern with nearly complete , meaning that individuals inheriting a single mutated from an affected have a high likelihood of developing the condition. The disorder is primarily caused by pathogenic variants in the SERPING1 gene, located on chromosome 11q12.1, which encodes the (C1-INH) protein, a key regulator in the complement, , and fibrinolytic systems. Nearly 1,000 distinct SERPING1 variants have been identified, contributing to the observed in HAE. In HAE type I, which accounts for approximately 85% of cases, lead to a quantitative deficiency of C1-INH, typically through null , frameshifts, nonsense , or large deletions that result in reduced . HAE type II, comprising about 15% of cases, involves qualitative defects where C1-INH levels are normal or elevated, but function is impaired due to missense that alter the protein's reactive center loop or other critical domains, thereby reducing its inhibitory activity. These SERPING1 disrupt C1-INH's role in controlling activity, though the downstream effects are distinct from the genetic mechanisms themselves. HAE type III, or HAE with normal C1-INH, is characterized by recurrent without C1-INH deficiency and is associated with gain-of-function mutations in the F12 gene encoding coagulation , which promotes excessive generation in subsets of patients. Additional genes implicated in type III HAE include PLG (encoding plasminogen), where specific gain-of-function variants lead to enhanced contact system activation through increased plasmin-mediated activation, and ANGPT1 (encoding angiopoietin-1), where loss-of-function variants cause reduced protein levels and impaired endothelial barrier stability; other genes such as HS3ST6 (encoding -glucosamine 3-O-sulfotransferase 6), where variants disrupt biosynthesis affecting signaling, account for a smaller proportion of cases and often show sensitivity. Type III HAE exhibits autosomal dominant but with incomplete , particularly in males. De novo mutations in SERPING1 occur in up to 25% of sporadic cases, highlighting the potential for the disorder to arise without family history, though familial transmission remains the primary mode. is recommended for all diagnosed individuals to assess inheritance risks, and family screening through targeted or clinical evaluation is advised to identify at-risk relatives early. In 2025, studies have expanded the genetic spectrum of HAE, identifying novel variants in non-SERPING1 genes such as KNG1 and MYOF across diverse populations, further elucidating the molecular basis in previously unexplained cases.

Pathophysiology

Hereditary angioedema (HAE) arises primarily from a deficiency or dysfunction of C1 inhibitor (C1-INH), a inhibitor that regulates multiple proteolytic cascades, including the complement, contact, fibrinolytic, and pathways. In its absence or impaired function, there is uncontrolled activation of the contact system, leading to excessive generation of , the central mediator of in HAE. This deficiency, often resulting from in the SERPING1 gene, reduces C1-INH levels or activity to approximately 5-30% of normal, thereby failing to inhibit key enzymes and perpetuating a of . The contact activation pathway begins with the autoactivation of (FXII) on negatively charged surfaces, forming FXIIa, which in turn converts prekallikrein to . Uninhibited by C1-INH, then cleaves (HMWK) at specific sites (Lys362 and Arg371), releasing (BK), a potent vasodilator . binds to bradykinin B2 receptors on endothelial cells, triggering intracellular calcium mobilization, release, and disruption of vascular endothelial junctions (e.g., via VE-cadherin ), which increases and allows plasma into subcutaneous and submucosal tissues, manifesting as nonpitting . Unlike histamine-mediated , HAE does not involve , which accounts for the absence of urticaria and the ineffectiveness of antihistamines or corticosteroids in treatment. The bradykinin generation pathway can be summarized as follows: \text{FXII} \xrightarrow{\text{autoactivation}} \text{FXIIa} \xrightarrow{\text{converts}} \text{prekallikrein} \to \text{kallikrein} \xrightarrow{\text{cleaves}} \text{HMWK} \to \text{bradykinin (BK)} Normally, C1-INH inhibits FXIIa and kallikrein to prevent excessive BK production; its dysregulation in HAE amplifies this pathway, with BK levels rising up to 10-fold during attacks. In type III HAE, where C1-INH levels and function are normal, the pathophysiology involves gain-of-function mutations in the F12 gene, which encodes FXII, leading to enhanced autoactivation and amplification of the bradykinin-producing cascade independent of C1-INH deficiency. These mutations promote fluid-phase activation of the contact system, resulting in dysregulated kallikrein activity and BK release similar to types I and II. Estrogen exacerbates this process by upregulating F12 expression, explaining the higher prevalence and severity of attacks in women, particularly during high-estrogen states such as pregnancy or oral contraceptive use.

Diagnosis and Classification

Types

Hereditary angioedema (HAE) is classified into subtypes primarily based on biochemical characteristics of C1 esterase inhibitor (C1-INH), a key regulatory protein in the complement, , and fibrinolytic systems, along with clinical and genetic features. The main types include HAE with C1-INH deficiency or dysfunction (types 1 and 2) and HAE with normal C1-INH (type 3), with all sharing recurrent episodes of nonpruritic subcutaneous or submucosal without urticaria. Type 1 HAE, accounting for approximately 85% of cases, is characterized by low antigenic levels of C1-INH (typically <50% of normal) and correspondingly reduced functional activity, resulting from quantitative deficiency due to in the SERPING1 gene. It affects males and females equally and often presents in childhood or . Type 2 HAE comprises about 15% of cases and features normal or elevated antigenic levels of C1-INH but reduced functional activity (<50% of normal) owing to the production of dysfunctional protein variants, also linked to SERPING1 mutations. Symptoms typically onset later than in type 1, often in adulthood, with similar clinical manifestations across sexes. Type 3 HAE, historically termed HAE with normal C1-INH, exhibits normal antigenic levels and functional activity of C1-INH and represents a rarer form that predominantly affects women, with attacks frequently triggered or exacerbated by estrogens such as during , , or oral contraceptive use. Subtypes are genetically heterogeneous, with the most common involving gain-of-function mutations in the F12 gene encoding ; less frequent variants include mutations in PLG (plasminogen), ANGPT1 (angiopoietin-1), KNG1, HS3ST6, and MYOF. Within HAE with normal C1-INH, cases with no identifiable genetic cause are termed HAE-UNK (unknown genetic ). Historically, types 1 and 2 have been grouped as C1-INH-HAE due to their shared biochemical deficiency or dysfunction, while type 3 is designated as normal C1-INH-HAE to reflect its distinct profile. Clinically, all types present with similar episodic swelling in the skin, , or upper airways, though type 3 more commonly involves recurrent facial and genital . Acquired angioedema, which mimics HAE but is not hereditary, must be distinguished and often arises later in life secondary to underlying conditions like or autoantibodies against C1-INH.

Diagnostic Approach

The diagnosis of hereditary angioedema (HAE) begins with clinical suspicion based on a history of recurrent episodes of affecting the skin, , or upper airways, characteristically without accompanying urticaria, and often unresponsive to standard treatments such as antihistamines or epinephrine. A positive history supports suspicion, though up to 25% of cases may arise from de novo mutations, and symptoms typically emerge in childhood or adolescence, sometimes triggered by stress, trauma, or infections. First-line screening involves measuring plasma levels, which are persistently low during symptom-free periods in types 1 and 2 HAE (accounting for approximately 85% of cases) and serve as a sensitive initial test for (C1-INH) deficiency, though they have limited specificity and are not diagnostic alone. Confirmatory testing requires assessment of C1-INH antigenic levels and functional activity: type 1 HAE is indicated by low C1-INH function (<50% of normal) with reduced antigenic levels, while type 2 HAE shows low function with normal or elevated antigenic levels. These tests should ideally be performed during quiescence to avoid false normals during acute attacks. For suspected type 3 HAE (HAE with normal C1-INH), and C1-INH levels and function are typically normal, necessitating targeted for variants in genes such as F12, PLG, or others to confirm the diagnosis in cases with high clinical suspicion. must exclude other forms of , including allergic or mast cell-mediated types (often with urticaria and responsive to antihistamines), inhibitor-induced , and acquired C1-INH deficiency (distinguished by low C1q levels and later onset). International guidelines, such as the 2025 HAE International (HAEi) consensus update, recommend screening at-risk first-degree family members with , C1-INH antigenic, and functional assays due to the autosomal dominant inheritance pattern. is advised for confirmation in ambiguous cases or for HAE with normal C1-INH. Challenges in diagnosis include normal laboratory results between attacks, leading to potential false negatives if testing occurs during or shortly after an episode, and delays in pediatric cases, with a mean diagnostic lag of 7.5 years often due to atypical presentations or misattribution of prodromal signs.

Management

Acute Attack Treatment

The treatment of acute hereditary angioedema (HAE) attacks focuses on rapid administration of targeted on-demand therapies to inhibit the bradykinin pathway and alleviate swelling, with intervention ideally initiated at the onset of symptoms to minimize duration and severity. Plasma-derived C1 esterase inhibitor (C1-INH) concentrates, such as Berinert (20 units/kg intravenously for adults) and Cinryze (1000 units intravenously), replace deficient or dysfunctional C1-INH to halt activation and bradykinin production; these are effective for abdominal, cutaneous, and laryngeal attacks, with median symptom relief onset in 0.5 to 2 hours compared to . Bradykinin B2 receptor antagonists, exemplified by icatibant (Firazyr; 30 mg subcutaneously, self-administrable in adults), competitively block effects at the receptor level, providing median time to 50% symptom improvement of about 2 hours versus nearly 20 hours with placebo; pediatric dosing is weight-based (0.4 mg/kg, maximum 30 mg). inhibitors like ecallantide (Kalbitor; 30 mg subcutaneously, administered by a healthcare provider) directly suppress activity, yielding significant symptom improvement by 4 hours post-injection in patients aged 12 years and older. A notable 2025 advancement is the approval of sebetralstat (EKTERLY; 600 mg orally), the first oral on-demand option for adults and adolescents aged 12 years and older, which reduces injection-related burden and achieves median symptom relief in 1.6 to 1.8 hours versus over 6 hours with , based on phase 3 data. Overall efficacy across these therapies typically manifests within 1 to 2 hours, with repeat dosing permitted (e.g., up to three doses in 24 hours) for persistent symptoms; however, antihistamines, corticosteroids, and epinephrine have no role, as HAE attacks are bradykinin-mediated rather than histamine-driven. For severe attacks, particularly laryngeal threatening airway patency, dosing may be adjusted to higher weight-based regimens (e.g., 20-30 units/kg for C1-INH in adults), and patients should receive for self-administration to enable prompt . Supportive care emphasizes airway protection, with fiberoptic preferred for respiratory compromise to secure ventilation without exacerbating ; tracheotomy is avoided when possible due to risks of worsening swelling and procedural complications, reserving for emergencies.

Prophylaxis

Prophylaxis for hereditary angioedema (HAE) encompasses strategies aimed at preventing attacks, divided into long-term prophylaxis for ongoing risk reduction and short-term prophylaxis for anticipated triggers. Long-term prophylaxis is recommended for patients experiencing frequent or severe attacks, typically defined as two or more attacks per year or those impacting , while short-term prophylaxis is used prior to high-risk events like or dental procedures.30878-3/fulltext) Long-term prophylaxis options include C1 esterase inhibitor (C1-INH) replacement , such as plasma-derived intravenous C1-INH (e.g., Cinryze at 1000 units infused twice weekly), which maintains functional C1-INH levels and reduces attack frequency by approximately 50-87% in clinical studies. Subcutaneous C1-INH formulations like Haegarda (20-60 /kg twice weekly) offer similar efficacy with greater convenience. Monoclonal antibodies targeting the kallikrein-kinin pathway, such as lanadelumab (Takhzyro, 300 mg subcutaneously every 2-4 weeks), inhibit plasma and achieve up to 87% reduction in attack rates. Androgen , including attenuated androgens like (50-200 mg orally daily), stimulate hepatic synthesis of C1-INH but are now second-line due to side effects such as , , and ; is less commonly used for similar reasons.30878-3/fulltext) Advances approved by the FDA in 2025 include donidalorsen (Dawnzera), an RNA-targeted antisense oligonucleotide administered subcutaneously every 4-8 weeks, which inhibits prekallikrein mRNA translation and reduces HAE attacks by over 90% in phase 3 trials, marking the first such therapy for prophylaxis in patients aged 12 and older. Garadacimab (Andembry), a monoclonal antibody targeting activated factor XII (FXIIa) for subcutaneous monthly dosing (200 mg), similarly achieves greater than 90% attack reduction by blocking the initial step of bradykinin formation and is approved for patients 12 years and older. Tranexamic acid (1-1.5 g orally daily) serves as an alternative for mild cases or during pregnancy, where it may reduce attacks by 50-70% through antifibrinolytic effects, though evidence is limited compared to first-line options.30611-2/fulltext) Short-term prophylaxis involves administering C1-INH (1000-2000 units intravenously) or (30 mg subcutaneously) 1-24 hours before invasive procedures to prevent attacks, with guidelines recommending this for dental work, , or stressful events in at-risk patients. Patient selection for prophylaxis is individualized based on attack frequency, severity, and factors, with regular for (e.g., attack logs) and side effects, including rare risk with C1-INH or liver function abnormalities with androgens. Discontinuation of androgens requires slow tapering over weeks to months to prevent rebound attacks due to sudden C1-INH level drops.30878-3/fulltext)

Outcomes and Epidemiology

Prognosis

With the advent of targeted therapies such as concentrates and inhibitors, the prognosis for patients with hereditary angioedema (HAE) has improved dramatically, reducing historical mortality rates from laryngeal attacks, which previously ranged from 15% to 30%, to less than 1% in those with access to modern treatments.30046-5/fulltext) Untreated laryngeal edema historically accounted for a lifetime risk of asphyxiation in approximately 20% to 30% of patients, but prophylactic regimens now mitigate this risk effectively, leading to near-normal comparable to the general population.01008-1/fulltext) The burden of attacks remains a key determinant of long-term outcomes, with untreated patients experiencing an average of 2 to 4 per month, often leading to chronic that results in unnecessary surgeries in up to 25% of cases and psychological sequelae including elevated rates of anxiety and due to unpredictable swelling episodes. With adherence to long-term prophylaxis, frequency typically decreases by 80% to 95%, resulting in fewer than 5 per year and substantial alleviation of these complications.00393-X/fulltext) Life expectancy is generally unaffected in managed cases, and pregnancy outcomes are favorable with tailored prophylactic strategies, yielding healthy deliveries in over 85% of reported instances without increased congenital risks. is further influenced by early , which reduces undiagnosed mortality risks by over 50%, reliable access to on-demand therapies, and consistent prophylaxis adherence, all of which minimize cumulative morbidity. As of 2025, emerging biologics like lanadelumab and donidalorsen have demonstrated attack reductions of 87% to 99% in clinical studies, correlating with quality-of-life improvements of 70% to 80% as measured by validated scales such as the Angioedema Control Test, enhancing overall patient well-being.

Epidemiology

Hereditary angioedema (HAE) has an estimated global of 1 in 50,000 individuals, though systematic reviews indicate a pooled rate of approximately 1.22 cases per 100,000 people, with variations due to underdiagnosis. The condition is underdiagnosed particularly in low-resource areas, where access to specialized testing and is limited, leading to apparent lower rates in regions like and compared to and . Incidence is equal between males and females for HAE types 1 and 2, which account for the majority of cases, while type 3 shows a marked female predominance of approximately 3:1, influenced by estrogen-related factors. Symptoms typically onset in childhood or , with about 75% of patients experiencing their first attack before age 15 and nearly all type 1 and 2 cases manifesting by age 20; in contrast, type 3 often begins post-puberty, around age 20 or later. Geographically, HAE distribution is uniform worldwide as an autosomal dominant disorder, but reporting is higher in and due to greater diagnostic awareness and infrastructure, with founder effects observed for certain SERPING1 mutations in specific populations such as those in . Ethnic variations show similar prevalence among populations in the United States (around 1.5 per 100,000), but lower reported rates in Asian and African descent groups, attributed to underdiagnosis rather than true rarity; recent 2025 analyses highlight increasing recognition of type 3 HAE in diverse ethnic cohorts globally. No specific comorbidities are uniquely associated with HAE, though type 3 carries an elevated risk of attacks triggered by estrogen-containing hormonal therapies due to its sensitivity to female sex hormones.

Societal and Research Aspects

Society and Culture

Hereditary angioedema (HAE) was first described as a distinct medical entity in by Heinrich Quincke, who documented cases of recurrent, nonpruritic subcutaneous affecting the skin and mucous membranes. In 1963, Virginia Donaldson and identified the underlying biochemical defect, linking HAE to a deficiency or dysfunction of C1 esterase inhibitor (C1-INH), a key regulator of the complement, , and fibrinolytic systems. The recognition of HAE type 3, characterized by normal C1-INH levels and function, emerged in 2000 through reports of affected families without the typical C1-INH abnormalities. The societal burden of HAE is substantial, with average annual healthcare costs exceeding $50,000 per in the United States, primarily driven by medications for acute attacks and long-term prophylaxis. Attacks often lead to work and school absenteeism, contributing to broader losses. These disruptions extend to family members, who may also experience indirect economic strain from caregiving responsibilities. Patient advocacy has played a pivotal role in advancing HAE care, exemplified by the founding of HAE International (HAEi) in 2004 as a global network of patient organizations focused on raising awareness, improving diagnosis, and ensuring access to treatments worldwide. HAEi collaborates with pharmaceutical companies and regulators to address unmet needs, including through initiatives that facilitate equitable distribution of therapies in underserved regions. The orphan drug designation granted by the U.S. (FDA) to multiple HAE therapies, such as berotralstat (Orladeyo) in 2020, has expedited development and approval processes for this rare condition, providing market exclusivity and financial incentives to encourage innovation. Cultural perceptions of HAE are influenced by its visible manifestations, such as disfiguring facial and extremity swelling, which can lead to , anxiety, and isolation among affected individuals due to misconceptions about the condition's contagiousness or severity. In HAE type 3, gender disparities are pronounced, with the disorder predominantly affecting women and exacerbated by hormonal fluctuations, particularly elevated estrogen levels during , , or oral contraceptive use, reflecting broader societal norms around female reproductive health. These factors can intensify emotional burdens, including and reduced , as women navigate diagnosis delays tied to underrecognition of estrogen's role. Media portrayals of HAE have increased visibility for this , with documentaries such as "Swell" (2011) chronicling patient experiences across generations and highlighting challenges, while "Special Blood" (2019) explores daily life impacts and efforts. In 2025, campaigns like the U.S. Hereditary Angioedema Association's (HAEA) Virtual initiative and HAEi's global access program have emphasized equitable availability, partnering with policymakers to reduce barriers in low-resource settings and promote worldwide awareness through events such as hae day. Economically, HAE patients face significant insurance challenges, including delays and denials for specialized treatments, which affect 70% of individuals and result in heightened attack frequency, increased emergency visits, and missed work or school days. These barriers often stem from the high cost of therapies and requirements, exacerbating financial strain and delaying optimal care despite the condition's potential for effective management.

Current Research

In 2025, several novel therapies for hereditary angioedema (HAE) received regulatory approvals, marking significant advancements in both on-demand and prophylactic treatment options. Sebetralstat, an oral plasma inhibitor, was approved by the U.S. (FDA) in July 2025 as the first oral on-demand therapy for acute HAE attacks in patients aged 12 years and older. Donidalorsen, an RNA-targeted antisense targeting prekallikrein, gained FDA approval in August 2025 for prophylactic use to prevent HAE attacks in adults and pediatric patients aged 12 years and older, offering quarterly or bimonthly subcutaneous dosing. In , the EMA's CHMP issued a positive for donidalorsen on November 16, 2025, recommending approval for HAE prophylaxis in patients aged 12 and older. Garadacimab, a inhibiting activated factor XIIa, was approved by the FDA in June 2025 for monthly prophylactic treatment of HAE attacks in patients aged 12 years and older, targeting an upstream component of the pathway. Ongoing clinical trials highlight promising candidates in the HAE pipeline. Deucrictibant, an oral , is advancing in phase 3 studies, including the CHAPTER-3 for prophylaxis and RAPIDe-3 for , with topline data expected in late 2025 and 2026, respectively. NTLA-2002, an investigational CRISPR-Cas9 -editing therapy targeting the KLKB1 to reduce production, demonstrated positive phase 1/2 results in 2025, showing deep and durable reductions in HAE attack rates and sustained normalization of function in treated patients. Genetic research in 2025 has expanded the understanding of HAE subtypes, particularly type 3 HAE with normal levels. Studies identified mutations in the ANGPT1 gene, encoding angiopoietin-1, and the MYOF gene, encoding myoferlin, as contributors to subsets of type 3 HAE, potentially disrupting pathways independent of the cascade. Additionally, genome-wide association studies have begun elucidating genetic modifiers that influence disease severity and treatment response across HAE types. Emerging research focuses on , pediatric applications, and long-term outcomes. levels have been validated as a potential for predicting and monitoring HAE attacks, with elevations correlating to active submucosal and aiding in distinguishing attacks from other conditions. Pediatric trials, such as the APeX-P of berotralstat, have shown and tolerability of early prophylaxis in children aged 2 to under 12 years, reducing attack rates with . Long-term safety data for biologics like garadacimab indicate a favorable profile, with durable attack prevention and minimal adverse events over extended use. Key challenges persist, including access disparities in low-income countries where diagnostic delays and lack of specialized care exacerbate unmet needs. Comparative effectiveness studies, such as network meta-analyses of prophylactic therapies, are underway to evaluate relative efficacy and quality-of-life impacts among options like lanadelumab and concentrates. Notable trials underscore these advances. The Ionis DAWNZERA open-label extension (OASISplus) reported a 94% mean reduction in HAE attack rates at one year with donidalorsen dosing every four or eight weeks. The Pharvaris CHAPTER-3 phase 3 trial is evaluating deucrictibant extended-release tablets for HAE prophylaxis, with enrollment ongoing and results anticipated in 2026.