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Calendula

Calendula is a of approximately 15–20 species of annual and perennial herbaceous plants belonging to the family, characterized by daisy-like flower heads with yellow to orange ray florets and central disk florets that may be yellow, red, or purple. Native to the Mediterranean region, including , , western , and , these plants typically feature erect or ascending stems, simple alternate leaves, and radiate inflorescences borne singly or in clusters. The most prominent species, (pot marigold), is extensively cultivated worldwide as an ornamental in gardens, borders, and containers due to its vibrant blooms that appear from through fall in cool weather. It grows 12–24 inches tall, with lance-shaped, glandular-hairy leaves and solitary flower heads up to 3 inches across, thriving in full sun and well-drained soil. Other species, such as C. arvensis and C. maritima, are less common but share similar botanical traits and are occasionally found in wild or coastal habitats. Beyond ornamentation, Calendula species, particularly C. officinalis, have a long history of ethnopharmacological use, with flowers containing , triterpenoids, , and essential oils that contribute to their , , and properties. Traditionally applied topically in ointments, creams, and infusions for , skin irritations, burns, and minor infections, these plants are recognized in for soothing mucous membranes and promoting tissue repair. Internally, preparations may aid digestive issues and inflammation, though clinical evidence supports primarily dermatological applications. Culinary uses of C. officinalis petals, which are and provide a subtle peppery taste and golden hue, include garnishing salads, soups, and rice dishes as a substitute, while the plant's dye properties yield and colors for fabrics and foods. Overall, Calendula exemplifies a versatile genus bridging , , and , with ongoing research exploring its bioactive compounds for modern therapeutic potential.

Botanical Overview

Description

Calendula species are herbaceous annuals or short-lived perennials in the family, typically reaching heights of 20–50 cm, though some can extend to 70 cm or more. They exhibit an erect growth habit with stems that branch from the base, forming bushy or lax structures supported by a system, and display the characteristic daisy-like appearance of the through their composite flower heads. Stems are often angular, solid, and range from glabrous to finely hairy or glandular, contributing to the plant's aromatic quality in cultivated forms. Leaves are alternate, simple, and oblong-lanceolate in shape, measuring 3–10 in length and 1–4 in width, with entire or weakly toothed, sometimes wavy margins; they are sessile or clasping at the base, glabrous or slightly hairy on both surfaces, and lower leaves may be more spatulate while upper ones are narrower. Inflorescences consist of solitary capitula, 3–7 in diameter, comprising one to two series of pistillate or sterile ray florets that are bright to and oblong-spatulate (15–25 mm long), surrounding bisexual florets with five-lobed corollas of similar coloration. Flowering generally spans from to autumn in temperate regions, with continuous blooming in suitable conditions until frost. Reproductive structures include heteromorphic achene fruits (cypselae), which are curved or boat-shaped, 5–10 mm long, yellowish to brownish, and often equipped with a pappus of scales or awns; outer achenes may be beaked or winged, while inner ones are more flattened. These shared morphological traits underpin the taxonomic of the genus. As an exemplar, , the pot , commonly features double-flowered cultivars in , where ray florets form multiple overlapping whorls for enhanced ornamental appeal.

Etymology

The genus name Calendula derives from the Latin calendae, referring to the first day of the month, a reference to the plant's prolific blooming that occurs nearly every month in Mediterranean climates. This etymological link highlights the flower's perceived reliability and continuity, evoking the structured passage of time marked by the ancient . In , the name calendula was applied to the in texts, predating modern botanical classification. The species was formally described and named by in his seminal work in 1753, establishing its place in the family. Linnaeus's solidified the genus, drawing on the longstanding Latin term to reflect its historical recognition. The common English name "" for stems from medieval associations with the Virgin ("Mary's gold"), but widespread confusion arose with the unrelated New World genus , also dubbed marigolds due to similar bright orange-yellow flowers. To differentiate, C. officinalis became known as "pot marigold," possibly alluding to its use in cooking pots or its pot-grown in gardens. The name Calendula remains conserved under the of Nomenclature for , fungi, and plants, preserving Linnaeus's designation for stability in scientific communication. In , the plant's near-constant flowering reinforced cultural ties to calendars and seasonal cycles.

Taxonomy and Diversity

Classification

Calendula belongs to the family (also known as Compositae), one of the largest families of flowering plants, encompassing over 32,000 species. Within this family, the genus is placed in the subfamily Asteroideae, which comprises the majority of Asteraceae diversity, and the tribe Calenduleae, a group primarily centered in with extensions to the Mediterranean region. Phylogenetic analyses position Calendula closely alongside genera such as , sharing a common ancestry within Calenduleae. A 2022 molecular study proposed subsuming Calendula into Osteospermum as Calendula, though this revision is not yet widely adopted. Molecular studies employing nuclear ribosomal (ITS) regions and plastid trnL-F intergenic spacers have robustly confirmed the monophyletic nature of Calendula, resolving its relationships and supporting its separation from more distant lineages. These sequence-based approaches highlight convergent evolutionary traits like winged cypselae across the tribe while underscoring Calendula's unique Mediterranean diversification. Historically, Calenduleae and its constituents, including Calendula, were sometimes aligned with broader helianthoid complexes in pre-molecular classifications, but revisions based on comprehensive morphological and anatomical data repositioned them firmly within Asteroideae. The modern framework adheres to the established in 2016, which integrates extensive cladistic evidence for family and tribal boundaries. Recent genomic sequencing efforts in the , including chromosome-level assemblies of , have reinforced this placement without necessitating major taxonomic overhauls, though they reveal insights into variations and pathways. Distinguishing features of Calenduleae, including Calendula, include the presence of secretory canals in stems and leaves, which produce oleoresins contributing to plant defense, and the accumulation of lactones—secondary metabolites characteristic of the tribe's chemical profile. These traits, observed through anatomical and analyses, aid in delimiting Calenduleae from neighboring tribes like Arctotideae, emphasizing the group's adaptation to arid and coastal environments.

Species

The genus Calendula encompasses approximately 15–20 accepted species of annual and perennial herbs, predominantly native to the Mediterranean Basin, Macaronesia, and extending to parts of western Asia and North Africa. These species exhibit considerable morphological and cytogenetic variation, reflecting their adaptation to diverse habitats from coastal dunes to inland steppes. Key representatives include C. officinalis L., the pot marigold, a short-lived perennial or annual widely cultivated for its ornamental and medicinal value; C. arvensis L., known as field marigold, an annual weed common in disturbed soils; C. maritima Guss., the sea marigold, a coastal perennial restricted to sandy habitats; and C. suffruticosa Vahl, a shrubby ornamental species with compact growth. Morphological distinctions among are evident in size, , and structure. For instance, C. officinalis features larger capitula measuring 4–7 cm in diameter, with bright yellow to ray florets, contrasting with the smaller 2–3 cm capitula of C. arvensis, which has more subdued yellow florets and a weedy, sprawling . C. maritima displays trailing stems and fleshy leaves adapted to saline environments, while C. suffruticosa forms subshrubs up to 50 cm tall with densely hairy foliage and persistent woody bases. Cytogenetically, vary in levels from diploid (e.g., C. stellata Cav., 2n = 2x = 14) to hexaploid, contributing to differences in stature and fertility. Biodiversity within Calendula is threatened by and , particularly for endemic . Several , such as C. stellata Cav. (Sicilian marigold, endemic to , , and northwest ), face risks from coastal development and , though it is assessed as Least Concern globally; regional evaluations highlight vulnerability due to limited populations. C. maritima, treated as a of C. suffruticosa in some classifications but recognized separately in conservation contexts, is listed as on the owing to severe habitat loss in western , with fewer than 250 mature individuals remaining. Other endemics like C. davisii A.C.Gonç. & P.Silveira () and C. eckerleinii Ohle () are narrow-range specialists susceptible to and pressures. Natural hybridization among Calendula species is infrequent in the wild, limited by ecological barriers and differing levels, though artificial crosses have been documented in . Domesticated forms of C. officinalis predominate in , with enhancing flower size and color diversity, often overshadowing wild relatives in .

Ecology and Cultivation

Habitat and Distribution

Calendula species are primarily native to the , encompassing and . For instance, , the pot marigold, originates from regions including , , , and extending eastward to the and southward to and other parts of . Other species, such as Calendula maritima, are more restricted, being endemic to coastal areas of western in and classified as due to habitat loss from coastal . These plants thrive in disturbed , including roadsides, waste grounds, and coastal dunes, where they colonize open, nitrogen-rich soils often resulting from human activity or natural . Calendula officinalis prefers full sun exposure and well-drained, sandy-loamy soils with a pH range of 6.0 to 7.0, though it can tolerate a broader spectrum from mildly acidic to alkaline conditions. C. maritima, in particular, adapts to saline, sandy substrates in coastal ecosystems, including mobile dunes and halophytic communities. Through cultivation and accidental introduction, Calendula has naturalized widely beyond its native range, appearing in temperate regions of the , , , and parts of . It has established self-sustaining populations in these areas, often escaping from gardens, though it is not typically considered highly invasive. Ecologically, Calendula species exhibit adaptations such as moderate , enabling survival in dry, sunny environments through efficient water use and antioxidant responses. They are primarily insect-pollinated, with flowers attracting and , thereby supporting local populations in both natural and managed landscapes. Additionally, by colonizing disturbed sites, these contribute to , helping to prevent on roadsides and dunes.

Growing Conditions

Calendula officinalis thrives in well-drained, fertile soil with a neutral to slightly alkaline , as this supports robust development and prevents waterlogging that can lead to . It requires full sun exposure of at least six hours per day for optimal flowering, though it tolerates some afternoon shade in hotter regions to avoid scorching. The plant is hardy as a short-lived in USDA zones 8 through 10, where mild winters allow overwintering, but it is typically grown as an annual in cooler climates (zones 2-7) due to sensitivity to hard freezes below 28°F (-2°C). Optimal growth occurs at temperatures between 15-25°C (59-77°F), with flowering best in cool to moderate conditions; excessive heat above 30°C (86°F) can reduce bloom quality and cause plants to bolt. Propagation is most commonly achieved by sowing seeds directly in the garden in early spring after the last frost, when soil temperatures reach 60°F (15.5°C); germination typically occurs within 7-14 days under these conditions. Calendula is self-seeding, readily dropping viable seeds that sprout the following season if allowed to mature, which supports naturalized plantings in suitable gardens. For perennial varieties or to maintain specific cultivars, softwood cuttings can be taken in spring or early summer, rooting quickly in moist, well-drained medium. Plants should be spaced 20-30 cm (8-12 inches) apart to allow for bushy growth and good air circulation, reducing disease risk. Ongoing care involves moderate watering to keep soil evenly moist but not soggy, providing about 1-1.5 inches per week during dry spells, as overwatering promotes fungal issues. Fertilizer needs are low; a balanced, slow-release application at planting suffices in fertile soil, with additional feeding unnecessary unless growth is stunted, as excess nutrients can lead to leggy plants with fewer flowers. Calendula exhibits good pest resistance but may attract aphids or slugs; these can be managed through hand removal, insecticidal soap, or beer traps without harming beneficial pollinators. To extend the blooming period from spring through fall, harvest or deadhead spent flowers every 2-3 days, which encourages continuous production of new buds. A 2025 study on examined the effects of end-of-day far-red light (700-750 nm) and solution formulas on Calendula in factory settings. Far-red light supplementation increased height and accelerated the appearance of the first flower. When combined with higher levels (3× standard Enshi formula) in hydroponic systems, it enhanced flower yield and use efficiency, though the and yield improvements were primarily due to the adjustments rather than far-red light alone.

Traditional Uses

Medicinal Applications

Calendula officinalis, commonly known as pot marigold, has been employed in for centuries, particularly for its soothing properties on and mucous membranes. Infusions prepared from its dried flowers were historically used to treat wounds, , and menstrual disorders, while ointments derived from the plant addressed conditions such as eczema and minor burns. These applications stem from ancient practices, as documented by Dioscorides in the CE, and herbal traditions across , , and the , where the flowers were valued for their ability to promote tissue repair and reduce swelling. The recognizes dried flower preparations of C. officinalis as a traditional medicinal product for the symptomatic relief of minor inflammations of and oropharyngeal mucosa, based on longstanding use. Oral preparations, such as teas made from the flowers, have been applied to alleviate symptoms of digestive ulcers by soothing irritated linings. Common include tinctures prepared at a 1:5 ratio in 90% , administered at 0.5–1 ml three times daily, and hydroalcoholic extracts incorporated at 10–20% concentration in topical creams for applications. Infusions from 1–4 g of dried flowers per cup of are used orally up to three times daily, while ointments typically contain 5–10% flower extract. Contraindications include to plants in the family, with caution advised during and due to insufficient safety data. Primarily, the flowers of C. officinalis are the focus of these medicinal applications, distinguishing it from other Calendula species.

Culinary and Dye Uses

Calendula petals are and commonly used in culinary applications to add color and a mild peppery or slightly bitter to dishes. They can be sprinkled fresh or dried onto salads, soups, preparations, and butters, providing a vibrant or hue similar to . Historically, calendula has served as an affordable substitute for , particularly for coloring foods like or , due to its golden pigmentation and subtle taste. Stems and leaves should be removed before consumption, as they tend to be more bitter. The flowers of yield natural producing shades of yellow, orange, and gold, suitable for coloring fabrics, yarns, and such as hair rinses. typically involves simmering the petals in hot or boiling water to release the pigments, though alcohol-based solvents can also be used for concentrated extracts. To improve color fastness on natural fibers, mordants like are applied, which brighten and fix the hues without significantly altering the base tones. Calendula is non-toxic and for consumption in food amounts by regulatory bodies like the FDA, though large quantities should be avoided to prevent potential mild effects from its traditional use in digestive remedies.

Chemical Composition

Major Constituents

flowers are rich in bioactive phytochemicals, with , triterpenoids, essential oils, , , and phenolic acids forming the primary constituents. These compounds vary in concentration depending on part, environmental factors, and methods, but flowers generally exhibit higher levels than leaves or stems. Analytical techniques such as (HPLC) and gas chromatography-mass spectrometry (GC-MS) are commonly employed to quantify and identify these components. Flavonoids, particularly flavonols such as , , isorhamnetin, and their glycosides (e.g., and calendoflavoside), constitute a significant portion of the fraction, reaching up to 4.7% of the dry weight in flowers (as quercetin equivalents) and contributing to the plant's vibrant yellow pigments. Concentrations of these compounds are notably lower in leaves. Carotenoids, including flavoxanthin, , and their esters, are abundant in the flowers, with total content ranging from 0.2% to 3.5% dry weight depending on and growing conditions, responsible for the characteristic orange-yellow coloration. Triterpenoids, including faradiol, arnidiol, and their esters (e.g., faradiol myristate and palmitate), represent the most abundant class, accounting for 0.5–1% of the dry flower weight in free and esterified forms, with total triterpenediol esters reaching up to 3–4% in the resinous fraction. These levels are substantially reduced in vegetative parts like leaves. Essential oils, comprising 0.1–0.3% of the dry flower weight, are dominated by sesquiterpenes such as α-cadinol, δ-cadinene, and α-cadinene (up to 18% of the oil). , present as (neutral and acidic types), provide a soothing matrix and are more concentrated in the flowers than in other tissues, though exact yields vary with extraction conditions. Phenolic acids, including , , and , occur in moderate amounts within the flowers, often quantified at several mg/g dry weight via HPLC, with being particularly prominent in methanolic extracts. Overall, flower extracts show greater diversity and higher totals of these constituents compared to leaves, as revealed by GC-MS profiling. These pigments from and also serve as natural dyes in traditional applications.

Biosynthesis

The biosynthesis of carotenoids in Calendula officinalis primarily occurs via the methylerythritol 4-phosphate () pathway in plastids, where isoprenoid precursors such as dimethylallyl pyrophosphate () and isopentenyl pyrophosphate () are synthesized and condensed to form geranylgeranyl diphosphate (GGPP), the immediate precursor for phytoene. Key enzymes in this pathway include phytoene synthase (PSY), which catalyzes the initial step of converting two GGPP molecules into phytoene, and is upregulated in flower petals to drive accumulation of colorful pigments like flavoxanthin and esters. Subsequent desaturation and steps, mediated by phytoene desaturase (PDS) and carotenoid (CRTISO), produce the characteristic 5-cis-configured abundant in orange petals, with CRTISO activity influencing petal coloration by modulating cis-trans . Triterpenoid biosynthesis in Calendula takes place in the through the mevalonate (MVA) pathway, leading to formation from , followed by cyclization via oxidosqualene cyclase (OSC) enzymes to generate cycloartenol as a primary intermediate. From cycloartenol, further oxidations and modifications yield faradiol, a key triterpenoid esterified with fatty acids, with specific OSC isoforms directing the and abundance of these pentacyclic structures in floral tissues. Genetic transformation using Agrobacterium rhizogenes has enabled the production of hairy root cultures in C. officinalis, achieving 100% transformation efficiency and enhancing triterpenoid yields through upregulated biosynthetic activity in these stable lines. Regulation of these pathways is influenced by environmental factors, including light quality and nutrient availability, which modulate ; for instance, far-red (FR) light supplementation boosts nutrient use efficiency (NUE) by optimizing and indirectly enhancing and triterpenoid production via phytochrome-mediated signaling. End-of-day red:far-red (R:FR) light treatments alter profiles, increasing levels in leaves and flowers through differential expression of biosynthetic genes. C. officinalis exhibits higher flux through these pathways compared to other Calendula species, correlating with elevated concentrations of and triterpenoids in its medicinal flowers. Analytical confirmation of these routes employs (NMR) spectroscopy for structural elucidation of intermediates like faradiol esters and isotopic labeling (e.g., 13C) to trace precursor incorporation in both MEP and MVA pathways.

Pharmacological Research

Therapeutic Effects

Calendula officinalis extracts exhibit effects primarily through the action of triterpenoids, such as faradiol, which inhibit the pathway, thereby reducing the transcription of pro-inflammatory genes. These triterpenoids also suppress (COX-2) expression, a key in synthesis, as demonstrated in animal models of acute and chronic inflammation where flower extracts significantly attenuated paw edema induced by or formalin. Furthermore, studies on cell lines show that ethanol extracts of Calendula flowers decrease production of cytokines including interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), linked to mechanisms. In , flavonoids like in stimulate synthesis by enhancing proliferation and upregulating transforming growth factor-beta (TGF-β) signaling in animal incision models, leading to improved tensile strength of healed . These compounds also promote , as observed in rat excisional wound studies where topical application accelerated vessel formation and epithelialization. Additionally, the properties contribute to (ROS) scavenging, with methanolic flower extracts showing an IC50 of approximately 100 μg/mL in DPPH assays, mitigating that impairs healing . Antimicrobial activity of extends to broad-spectrum inhibition against like Staphylococcus aureus and fungi such as Candida albicans, with ethanolic petal extracts showing antimicrobial activity against clinical isolates in broth dilution assays. This effect is attributed to membrane disruption by triterpenoids and , as evidenced by reduced formation in S. aureus cultures treated with extracts at sub-MIC levels. Beyond these, demonstrates antidiabetic potential via inhibition of α-glucosidase, an enzyme involved in ; flower extracts achieve up to 43% inhibition at 500 μg/mL in enzymatic assays, comparable to standards. For anticancer effects, studies on models indicate induction of through caspase-3 activation and downregulation, with extracts reducing viability by approximately 50% at 50-100 μg/mL, as summarized in 2023-2024 reviews.

Clinical Studies

Clinical studies on have primarily focused on its topical applications for and dermatological conditions, with evidence from randomized controlled trials (RCTs) and s supporting moderate efficacy in accelerating recovery while demonstrating a favorable profile. In care, a 2016 RCT involving patients with venous leg ulcers (n=57; 38 treatment, 19 control) found that topical application of a C. officinalis extract resulted in a significant four-fold increase in weekly velocity (7.4% vs. 1.7% in the control group), alongside reduced ulcer area and improved metrics. A 2019 of 14 (7 preclinical and 7 clinical) studies concluded that C. officinalis flower extract monotherapy promotes faster closure compared to controls in acute wounds, with statistically significant improvements observed as early as five days post-injury in human trials, though mixed results for chronic wounds and the authors noted the need for larger RCTs to confirm effect sizes. More recent analyses, such as a 2023 review, highlight consistent benefits in reducing and risk in various types, including burns and surgical incisions, without identifying a specific quantifying overall acceleration. As of 2025, additional reviews confirm these effects and explore new applications, such as protection against and via . For dermatological applications, particularly radiation-induced , a landmark Phase III RCT (n=254) demonstrated that C. officinalis ointment significantly reduced the incidence of acute during radiotherapy (41% vs. 63% for trolamine control; p<0.001), with fewer interruptions in treatment. However, a 2020 RCT (n=100) in head and neck cancer patients reported no significant difference in dermatitis prevalence or severity between topical C. officinalis (10% extract) and standard sorbolene cream, suggesting variability in efficacy depending on formulation and patient population. Follow-up data from ongoing observational studies up to 2024 reinforce its role in symptom palliation, with reductions in erythema and pruritus reported in up to 40% of cases in responsive cohorts. Safety data from clinical trials indicate low rates of adverse events, primarily mild local irritation in less than 5% of participants, with no systemic toxicity observed in short-term use. Genotoxicity assessments, including on isolated saponins and extracts, have been negative, supporting non-mutagenic potential. Drug interactions are rare but include potentiation of sedative effects, such as increased drowsiness when combined with or other CNS depressants, warranting caution in concurrent use. Recent updates address research gaps, including 2023-2025 studies on oral C. officinalis administration, which show promise in enhancing burn wound healing via systemic anti-inflammatory effects in small RCTs (n=60), though bioavailability remains limited (estimated 8-47% for key triterpenoids) and requires optimization through formulations like nanoparticles. Combined therapies, such as C. officinalis with honey, are under investigation for synergistic wound benefits, but long-term human data (>6 months) on chronic use and potential allergenicity in sensitive populations remain sparse.

Historical and Cultural Significance

Ancient and Medieval History

The use of , commonly known as pot marigold, dates back to and times, where it was valued for its medicinal properties. In the 1st century CE, the Greek physician documented the plant in his seminal work , recommending it for treating wounds and due to its cooling and qualities derived from the flowers. During the medieval period, gained prominence in European monastic gardens and was cultivated since the for both ornamental and therapeutic purposes. The 12th-century German abbess and visionary Hildegard von Bingen described the plant extensively in her medical writings, prescribing calendula-based ointments for skin conditions, highlighting its role in monastic healing practices. In Islamic medicine, the scholar (Ibn Sina) included calendula in his (11th century), advocating its use as a blood purifier, underscoring its integration into diverse medieval healing systems across . By the early modern era, calendula had spread to , including , where it was present by the , possibly introduced via trade or monastic gardens from the Mediterranean. English herbalist John Gerard praised its efficacy for healing sores, wounds, and inflammations in his influential 1597 Herball or Generall Historie of Plantes, solidifying its reputation as a versatile remedy. Beyond medicine, the vibrant yellow-orange petals were employed as a for fabrics and textiles, contributing to the coloration of medieval and early modern tapestries and garments, though less potently than .

Modern Recognition

In the 19th century, Calendula officinalis experienced a revival in homeopathic and phytotherapeutic practices, notably through the work of , who documented its use for and in his Materia Medica Pura (1811–1821), influencing subsequent homeopathic formularies. Commercialization accelerated in the 1920s with the founding of in 1921, which introduced Calendula ointment as a staple anthroposophic remedy, drawing on Rudolf Steiner's principles and still widely available today. Contemporary recognition has solidified Calendula's status in regulated , with the () issuing a in 2008 for its flowers as a traditional herbal medicinal product for minor wounds and , revised in 2018 to incorporate new safety data and quality standards. In the , it features prominently in various formulations for sensitive soothing, reflecting its emollient and anti-irritant properties in over-the-counter products. Culturally, Calendula symbolizes grief and sorrow in the Victorian , as outlined in 19th-century floriography texts like Henry ' Flora Historica (1824), where it represented despair in bouquets. In recent years, its economic importance has grown through global trade in products, with countries like and as notable producers and exporters of Calendula for medicinal and cosmetic uses. As of 2025, ongoing explores its applications in and modern therapeutics, bridging historical traditions with contemporary practices.