Estrous cycle
The estrous cycle is the recurring reproductive cycle in most female mammals, characterized by periodic changes in the ovaries and reproductive tract that prepare the body for mating, ovulation, and potential pregnancy, with sexual receptivity limited to a brief period known as estrus.[1] Unlike the menstrual cycle in humans and some primates, it lacks significant shedding of the uterine lining, instead involving reabsorption of the endometrium if conception fails.[2] The cycle begins at sexual maturity and is interrupted only by pregnancy or anestrous periods, varying in length from 3 to 21 days across species, with larger mammals generally exhibiting longer cycles due to extended luteal phases.[1] The estrous cycle is divided into four distinct phases: proestrus, estrus, metestrus, and diestrus, each driven by fluctuating hormone levels that coordinate follicular development, ovulation, and preparation of the reproductive tract.[1] During proestrus, the corpus luteum from the previous cycle regresses, leading to declining progesterone levels, while follicle-stimulating hormone (FSH) stimulates the growth of ovarian follicles, causing a rise in estrogen (estradiol) that proliferates the endometrium and prepares the female for mating.[3] Estrus follows, marked by peak estrogen levels that trigger a luteinizing hormone (LH) surge from the anterior pituitary, inducing ovulation and rendering the female sexually receptive, often accompanied by behavioral signs like mounting or vocalization and physiological changes such as vaginal cornification.[3] In metestrus, the ruptured follicle transforms into a new corpus luteum under LH influence, initiating progesterone production, while if fertilization occurs, the ovum travels to the uterus.[1] Diestrus represents the luteal phase, where the corpus luteum functions optimally, secreting high levels of progesterone that maintain the endometrium in a secretory state and inhibit further follicular development through negative feedback on gonadotropin-releasing hormone (GnRH) from the hypothalamus.[3] Hormonal regulation of the estrous cycle involves intricate feedback loops between the hypothalamus, pituitary gland, and ovaries, ensuring synchronized events.[3] GnRH pulses from the hypothalamus stimulate the release of FSH and LH, which in turn drive ovarian steroidogenesis; estrogen exerts positive feedback during proestrus to amplify the preovulatory LH surge, while progesterone provides negative feedback during diestrus to suppress GnRH and gonadotropins, preventing premature ovulation.[3] If pregnancy does not occur, prostaglandins from the uterus trigger luteolysis (corpus luteum regression), dropping progesterone and restarting the cycle.[3] This system contrasts with the menstrual cycle's more prolonged follicular phase and overt bleeding due to endometrial necrosis from progesterone withdrawal, highlighting evolutionary adaptations in non-primate mammals for efficient reproduction without energy loss from menstruation.[2]Introduction and Fundamentals
Definition and Overview
The estrous cycle refers to the recurring physiological changes in the reproductive tract of female non-primate mammals, induced by reproductive hormones, that lead to periods of estrus (heat) and ovulation.[4] This cycle represents the primary reproductive process in these species, distinguishing it from the menstrual cycle observed in higher primates.[5] The core purpose of the estrous cycle is to synchronize female behavioral receptivity to mating with ovarian follicle development and the preparation of the reproductive tract for potential pregnancy.[1] These coordinated changes ensure that ovulation occurs during a discrete window of fertility, optimizing reproductive success by aligning physiological readiness with opportunities for conception.[6] The duration of the estrous cycle varies widely among non-primate mammalian species, typically from 4-5 days in rodents to about 21 days in ruminants and horses, contrasting with the more continuous estrus-like receptivity in primates.[5] The cycle involves key anatomical structures, including the ovaries for follicle maturation and ovulation, the uterus for endometrial changes, and the hypothalamus-pituitary axis for hormonal orchestration.[4] It comprises distinct phases—proestrus, estrus, metestrus, and diestrus—that drive these reproductive events, with an optional anestrus period of reproductive quiescence.[7]Etymology and Nomenclature
The term "estrous cycle" derives its first component from the noun "estrus," which originates from the Latin oestrus meaning "frenzy" or "gadfly," itself borrowed from the Ancient Greek oîstros (οἶστρος), denoting a gadfly, sting, breeze, or mad impulse that drives animals into a frenzied state, metaphorically applied to the intense sexual behavior observed in females during heat.[8] The second component, "cycle," comes from the Late Latin cyclus and Old French cicle, ultimately from the Greek kyklos (κύκλος) meaning "circle" or "wheel," signifying a recurring series of events or operations.[9] The nomenclature was formalized in the early 20th century by British zoologist Walter Heape, who in 1900 introduced the term "estrous cycle" (spelled "oestrous" in British English) to describe the reproductive periodicity in non-primate mammals, explicitly distinguishing it from the human menstrual cycle based on observable behavioral and physiological patterns.[10] Heape's work emphasized the cycle's role in the "sexual season" of mammals, coining phase-specific terms such as proestrus (the preparatory period leading into heat), estrus (the peak of sexual receptivity), metestrus (the immediate post-heat subsidence), diestrus (a brief rest within the breeding season), and anestrus (the extended non-breeding rest period).[11] In modern usage, "estrous" is the standard American English spelling, while "oestrous" persists in British English, reflecting orthographic conventions for words derived from Latin and Greek roots.[12] This terminology contrasts with the "menstrual cycle," which Heape and subsequent researchers reserved for primates exhibiting overt bleeding, whereas estrous cycles in other mammals are characterized by behavioral "heat" without such menstruation.[11] Colloquially, the estrous cycle is often referred to as "heat," "rut," or "breeding season" in veterinary and agricultural contexts, terms that capture the female's limited window of fertility and mating willingness.[12]Comparison to Menstrual Cycle
Key Differences
The estrous cycle, characteristic of most non-primate mammals, differs fundamentally from the menstrual cycle observed in humans and some other primates in the handling of uterine tissue. In the estrous cycle, the endometrium undergoes resorption or reorganization if pregnancy does not occur, without significant shedding or visible bleeding, conserving energy and minimizing physiological costs.[13] In contrast, the menstrual cycle involves the sloughing off of the endometrial lining, resulting in menstrual bleeding, which is a response to progesterone withdrawal in the absence of implantation.[2] This absence of overt discharge in estrous species reflects an adaptation to maintain reproductive efficiency without the need for extensive tissue regeneration each cycle.[14] Cycle continuity also sets the estrous and menstrual cycles apart. Estrous cycles can be polyestrous (either continuous year-round or multiple within defined breeding seasons), seasonally polyestrous, or monoestrous annually, with many aligning with environmental cues like photoperiod to concentrate reproductive efforts.[15] For instance, many mammals exhibit multiple estrous cycles during a favorable period, followed by anestrus, whereas the menstrual cycle operates continuously year-round, independent of seasons, allowing for more frequent reproductive opportunities.[13] This seasonal patterning in estrous cycles optimizes resource allocation for offspring survival in variable environments.[16] Behaviorally, the estrous cycle features pronounced sexual receptivity confined to the brief estrus phase, often marked by overt signs like vocalizations or postures that signal readiness for mating, tightly coupling behavior to ovulation.[2] The menstrual cycle decouples these elements, with females potentially receptive throughout the cycle, not solely during fertile periods, which supports social bonding in primate groups.[13] Evolutionarily, the estrous cycle represents an adaptation for species with discrete breeding seasons, synchronizing reproduction with optimal conditions for offspring viability, such as abundant food or milder weather, thereby enhancing survival rates in non-primate mammals.[14] Menstrual cycles, conversely, evolved in lineages favoring continuous cycling, possibly to facilitate embryo selection and protection against suboptimal pregnancies through spontaneous decidualization.[17] If conception fails, outcomes diverge markedly: the estrous cycle may result in quiet ovulation or pseudopregnancy, with the corpus luteum regressing without endometrial disruption, leading seamlessly to the next cycle or anestrus.[13] In the menstrual cycle, non-pregnancy triggers menstruation, clearing the uterus for a new proliferative phase.Physiological Similarities
The estrous and menstrual cycles share fundamental physiological mechanisms that underscore their evolutionary conservation across mammals, enabling reproductive success through coordinated ovarian and uterine changes. Both cycles feature an ovarian component involving the sequential development of ovarian follicles, ovulation of a mature oocyte, and subsequent formation of the corpus luteum, which supports potential pregnancy by secreting progesterone. In estrous cycles of species like rodents and dogs, follicles progress from primordial to antral stages under gonadotropin influence, culminating in ovulation triggered by a luteinizing hormone (LH) surge, followed by luteinization of granulosa and theca cells into the corpus luteum; similarly, in the menstrual cycle of primates including humans, follicle-stimulating hormone (FSH) drives follicular maturation, with ovulation occurring mid-cycle and the corpus luteum forming post-ovulation to maintain the endometrium. These processes ensure the release of gametes at optimal times for fertilization.[18][19] Hormonal regulation in both cycles is governed by the hypothalamus-pituitary-ovarian (HPO) axis, which integrates positive and negative feedback loops to orchestrate cyclicity. The hypothalamus releases gonadotropin-releasing hormone (GnRH) in pulsatile fashion to stimulate pituitary secretion of FSH and LH, which in turn promote ovarian steroidogenesis; rising estradiol levels exert positive feedback to induce the pre-ovulatory LH surge for ovulation, while negative feedback from progesterone and inhibin suppresses gonadotropins during the luteal phase to prevent premature follicle recruitment. This axis operates comparably in estrous mammals, such as rats and sheep, where feedback dynamics maintain short cycles, and in menstrual cycles, sustaining longer phases with similar steroid-mediated control.[19] Uterine preparation for implantation exhibits parallel endometrial responses driven by ovarian hormones in both cycles. Estrogen stimulates endometrial proliferation during the follicular/proestrus phase, thickening the lining through glandular and stromal cell growth to create a receptive environment; progesterone from the corpus luteum then induces secretory changes for nutrient support if fertilization occurs. In estrous cycles, such as in dogs, this proliferation peaks pre-ovulation without overt bleeding, while in menstrual cycles, it prepares the endometrium for potential decidualization, highlighting conserved estrogen-progesterone synergy for implantation.[1][19] The duration of the fertile window aligns closely relative to overall cycle length in both systems, with ovulation marking the peak fertility period typically 12-24 hours after the LH surge, and viable spermatozoa surviving up to several days prior. In a standard 28-day menstrual cycle, ovulation occurs around day 14, yielding a 5-6 day fertile window; analogously, in the 4-5 day rodent estrous cycle, ovulation follows proestrus, confining fertility to estrus with similar relative timing for conception. This synchronization optimizes gamete encounter across species.[20][21] At the molecular level, core genes encoding receptors for gonadotropins, such as FSH receptor (FSHR) and LH/choriogonadotropin receptor (LHCGR), demonstrate high conservation across mammals, facilitating uniform signaling in reproductive cycles. FSHR, a G-protein-coupled receptor, mediates FSH-driven folliculogenesis with conserved transmembrane domains and key residues (e.g., Asp224) essential for activation in both estrous and menstrual contexts; LHCGR similarly supports LH-induced ovulation and luteal function through shared structural motifs, ensuring robust hormone responsiveness from rodents to primates.[22]Phases of the Cycle
Proestrus
The proestrus phase represents the preparatory stage of the estrous cycle in female mammals, during which the reproductive system undergoes changes to support follicle maturation and impending ovulation. This phase is marked by the regression of the corpus luteum from the previous cycle, leading to declining progesterone levels and the initiation of follicular development in the ovaries. As a result, circulating estrogen concentrations begin to rise, setting the stage for subsequent phases.[7] The duration of proestrus varies significantly across species, typically spanning 2-4 days in many domestic mammals, though shorter in rodents (e.g., 12-24 hours in rats and mice) and longer in others (e.g., 6-11 days in dogs). In cattle, it lasts 1-3 days, characterized by follicular waves where multiple follicles grow but only one dominant follicle emerges. Physiological changes include accelerated growth of ovarian follicles stimulated by rising follicle-stimulating hormone (FSH) from the anterior pituitary, with granulosa cells in the developing follicles increasingly producing estradiol. This estrogen surge promotes endometrial proliferation in the uterus, thickening the lining through cellular mitosis to prepare for potential embryo implantation, while also influencing cervical mucus production to become more watery and fertile.[23][24][7][25] Behaviorally, females during proestrus show limited sexual receptivity to males, distinguishing this phase from the receptive estrus that follows, though they may display increased playfulness or attraction in some species like dogs. Common physical signs include vulvar swelling due to estrogen-induced vascularization and clear or slightly bloody vaginal discharge, which aids in lubrication and signals approaching fertility; in rodents, the vagina appears swollen and moist with nucleated epithelial cells observable in smears. These changes reflect the mounting estrogen influence on accessory reproductive structures.[24][7] Hormonally, proestrus is triggered by pulsatile gonadotropin-releasing hormone (GnRH) from the hypothalamus, which stimulates the pituitary gland to release escalating levels of FSH and luteinizing hormone (LH). The rising estrogen provides positive feedback to the hypothalamus and pituitary, amplifying GnRH and gonadotropin secretion. This phase endpoints with a critical preovulatory LH surge, typically occurring toward the close of proestrus, which induces final oocyte maturation, ovulation within 24-36 hours, and the shift to estrus.[3][25][7]Estrus
Estrus, also known as "heat," represents the fertile phase of the estrous cycle in female mammals, marked by peak sexual receptivity, ovulation, and physiological adaptations that facilitate mating and conception.[19] This phase follows the preparatory follicular development in proestrus and is the period when the female is most likely to accept a male for copulation, optimizing the chances of successful fertilization.[7] In general, estrus is the shortest phase of the cycle, typically lasting 1-2 days across many mammalian species, though durations vary; for instance, it may extend to 2-3 days in swine or be as brief as 12-18 hours in cattle.[19][26] Physiologically, estrus is characterized by the culmination of follicular maturation, with ovulation occurring as the dominant event, triggered by a preovulatory surge in luteinizing hormone (LH) that typically happens toward the end of proestrus but results in egg release during early estrus.[7] The reproductive tract undergoes key changes to support sperm transport and survival: cervical mucus becomes abundant, watery, and sperm-friendly with a characteristic ferning pattern that enhances motility, while uterine contractions increase to propel sperm toward the oviducts.[19] These adaptations, driven by elevated estrogen levels reaching their maximum, create an optimal environment for fertilization shortly after insemination.[26] Behaviorally, females exhibit pronounced signs of receptivity during estrus, including attraction to males, increased vocalizations, and specific postures such as lordosis in rodents or "standing heat" in many ungulates like cattle and swine, where the female rigidly stands to allow mounting.[19] These behaviors are estrogen-mediated and signal peak fertility, often accompanied by restlessness, frequent mounting of other females, and vulvar swelling or discharge.[26] Hormonally, estrus follows the LH surge, with estrogen concentrations at their zenith to sustain receptivity, while progesterone levels begin to rise post-ovulation as the corpus luteum starts forming, marking the transition to subsequent phases.[7] The fertility window during estrus is narrow but critical, with optimal insemination timing aligned to the period of receptivity, as the released ova remain viable for approximately 12-24 hours in most mammals.[19] This brief viability underscores the importance of synchronized mating, with sperm survival in the female tract potentially extending up to 48 hours in some species to overlap with ovulation.[7]Metestrus
Metestrus represents the immediate post-ovulatory phase of the estrous cycle in mammals, typically lasting 2 to 4 days, as observed in species such as cattle and sheep.[27][28] This brief interval follows the cessation of estrus and is characterized by the onset of luteal development following ovulation, which occurs approximately 10 to 15 hours after the end of behavioral receptivity in bovines.[27] Physiologically, the corpus hemorrhagicum—a blood clot-filled structure—forms within the ovarian follicle's rupture site, serving as the precursor to the corpus luteum through luteinization of granulosa and theca cells.[27][29] This early corpus luteum initiates low-level progesterone secretion, which gradually rises but remains insufficient for full luteal support initially, while circulating estrogen concentrations decline sharply from estrus peaks.[27][30] Behaviorally, sexual receptivity diminishes quickly, with females exhibiting reduced attraction to males and no further mating attempts, signaling the end of the fertile window.[27][31] In the uterus, rising progesterone prompts initial endometrial changes, including glandular proliferation and vascular remodeling, to prepare for embryo implantation should fertilization occur.[32][33] However, without an embryo, these preparatory alterations remain transient and reversible, allowing the reproductive tract to reset for subsequent cycles.[34] If conception fails, early signs of pseudopregnancy—such as mild mammary gland development—may emerge in certain species like dogs, driven by sustained progesterone influence.[35] Overall, metestrus functions as a critical bridge from the ovulatory events of estrus to the sustained luteal dominance of diestrus, stabilizing the post-ovulatory environment while maintaining reproductive plasticity.[27][36]Diestrus
Diestrus represents the luteal phase of the estrous cycle in mammals, characterized by the dominance of progesterone secreted by the mature corpus luteum, which supports potential implantation and early pregnancy or regresses if fertilization does not occur.[37] This phase typically lasts 10-14 days in many species, making it the longest segment of the cycle, though durations vary; for instance, it spans approximately 12 days in cattle and 14-15 days in mares.[27][38] Following the brief metestrus transition, the corpus luteum fully matures during diestrus, inhibiting the development of new ovarian follicles through negative feedback on the hypothalamic-pituitary axis.[37] Physiologically, the elevated progesterone prepares the reproductive tract for gestation, thickening the endometrium and promoting glandular secretions in species like cattle, while suppressing further ovulation.[27] If pregnancy occurs, progesterone sustains the corpus luteum and initiates mammary gland development for lactation, as seen in livestock such as pigs and cows.[39] Hormonally, progesterone levels rise to and maintain above 5 ng/mL—reaching 30-40 ng/mL in pigs—suppressing gonadotropin-releasing hormone (GnRH), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) release, thereby preventing premature follicular growth.[37][40] Behaviorally, females exhibit no sexual receptivity during diestrus, often displaying increased aggression or, if pregnant, early maternal tendencies, as progesterone overrides estrogen-driven behaviors observed in prior phases.[38] The phase concludes with luteolysis, the regression of the corpus luteum triggered by uterine-derived prostaglandin F2α (PGF2α) in non-pregnant animals, leading to a sharp decline in progesterone and the onset of proestrus; this process is well-documented in ruminants like cattle, where PGF2α pulses initiate the next cycle.[27][39]Anestrus
Anestrus represents the phase of reproductive dormancy in the estrous cycle of many mammals, marked by a complete cessation of follicular development, ovulation, and behavioral estrus, with females exhibiting indifference or resistance to mating advances.[41] During this period, the reproductive tract remains quiescent, including small, inactive ovaries and a uterus with minimal endometrial activity.[42] This phase contrasts with the active cycling periods of proestrus, estrus, metestrus, and diestrus by imposing a prolonged interval of infertility.[43] The duration of anestrus varies widely across species and environmental contexts, typically ranging from several weeks to months, and is often pronounced in seasonal breeders from temperate regions.[42] For instance, in long-day breeders such as horses and donkeys, anestrus may last 3–5 months during autumn and winter, while in short-day breeders like sheep, it occurs over summer, spanning 2–3 months.[44] Postpartum anestrus can extend longer, such as 12–24 months in elephants under natural conditions, though it shortens with reduced stress.[41] Physiologically, anestrus features suppressed secretion of gonadotropin-releasing hormone (GnRH) from the hypothalamus, resulting in low circulating levels of luteinizing hormone (LH) and follicle-stimulating hormone (FSH).[42] This leads to ovarian inactivity, with follicles undergoing atresia rather than maturation, and correspondingly low concentrations of estradiol and progesterone.[44] Uterine involution occurs, minimizing metabolic demands on the reproductive system.[43] Key causes of anestrus include environmental cues that inhibit hypothalamic-pituitary-gonadal axis activity, such as shortened photoperiods in winter for long-day breeders, which elevate melatonin and dampen GnRH pulsatility.[42] Nutritional deficits, signaling low energy availability through pathways like AMPK activation, further suppress GnRH release, while stress-induced cortisol elevation from the hypothalamic-pituitary-adrenal axis reinforces this inhibition.[44] The primary role of anestrus is to promote energy conservation, allowing mammals to allocate resources toward survival rather than reproduction during periods of environmental adversity, such as food scarcity or harsh weather in non-breeding seasons.[42] This adaptive strategy ensures that breeding resumes only when conditions favor offspring viability, as seen in temperate species like sheep and hamsters.[44]Hormonal Regulation
Major Hormones Involved
The estrous cycle in mammals is orchestrated by a coordinated network of hormones that regulate follicular development, ovulation, and preparation for potential pregnancy. These hormones originate from the hypothalamus, anterior pituitary gland, ovaries, and uterus, with their pulsatile secretion driving the cyclic changes in reproductive physiology. Key players include gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (the primary estrogen), progesterone, and prostaglandin F2α (PGF2α).[45] Gonadotropin-releasing hormone (GnRH) is secreted by neurons in the hypothalamus in a pulsatile manner, serving as the master regulator of the reproductive axis. It stimulates the anterior pituitary to release FSH and LH, thereby initiating and synchronizing ovarian events across the cycle phases. Disruptions in GnRH pulsatility can lead to irregular estrous cycles in various species.[45][46] Follicle-stimulating hormone (FSH), produced by gonadotroph cells in the anterior pituitary, promotes the growth and maturation of ovarian follicles during the early stages of the cycle. FSH acts on granulosa cells to enhance follicular development and stimulate the production of estrogens, setting the stage for subsequent ovulation. Levels of FSH typically rise at the onset of the cycle to recruit multiple follicles, with the dominant one selected for further growth.[47][48] Luteinizing hormone (LH), also secreted from the anterior pituitary, plays a pivotal role in triggering ovulation and supporting luteal function. A surge in LH, induced by rising estrogen levels, causes the rupture of the mature follicle and the release of the oocyte, typically occurring around the estrus phase. Post-ovulation, LH maintains the corpus luteum, ensuring progesterone production to sustain the luteal phase.[49][50] Estradiol, the predominant form of estrogen, is synthesized by granulosa cells in developing ovarian follicles under the influence of FSH. It exerts positive feedback on the hypothalamus and pituitary to induce the LH surge, promotes endometrial proliferation during proestrus, and elicits behavioral estrus in females. Peak estradiol levels correlate with receptivity to mating and prepare the reproductive tract for fertilization.[1][49] Progesterone is primarily produced by the corpus luteum following ovulation, induced by LH. It maintains the uterine environment during the luteal phase (diestrus), inhibits further follicular development, and supports early pregnancy if conception occurs. Declining progesterone levels signal the regression of the corpus luteum, allowing the cycle to restart.[51][52] Prostaglandin F2α (PGF2α), synthesized by the uterine endometrium, is crucial for luteolysis at the end of the luteal phase. Released in pulses around days 16-18 of the cycle in species like cattle, it induces the functional and structural breakdown of the corpus luteum, thereby reducing progesterone and facilitating the return to follicular development. This process ensures cyclic fertility in non-pregnant animals.[53]Molecular Mechanisms
The molecular mechanisms governing the estrous cycle operate primarily through receptor-mediated signaling and transcriptional regulation at the cellular level. The gonadotropin-releasing hormone (GnRH) receptor, a prototypical G-protein-coupled receptor (GPCR), facilitates the pulsatile release of GnRH from hypothalamic neurons, activating downstream phospholipase C pathways to mobilize intracellular calcium and protein kinase C.[54] Similarly, the luteinizing hormone (LH) and follicle-stimulating hormone (FSH) receptors, also members of the GPCR superfamily, are expressed on granulosa and theca cells in the ovary, where they couple to Gs proteins to stimulate adenylate cyclase, elevating cyclic AMP levels and promoting steroidogenesis.[55] In parallel, estrogen and progesterone receptors function as nuclear receptors, translocating to the nucleus upon ligand binding to modulate chromatin structure and directly influence the transcription of target genes involved in reproductive tissue proliferation and differentiation.[56] Feedback loops at the molecular level ensure precise temporal control of the cycle. Positive feedback arises from escalating estrogen concentrations, which activate estrogen receptor alpha in kisspeptin neurons within the anteroventral periventricular nucleus of the hypothalamus, triggering a surge in GnRH and subsequent LH release to induce ovulation.[57] Conversely, negative feedback is exerted by progesterone binding to its nuclear receptors, which represses GnRH neuronal activity through inhibition of kisspeptin expression and enhancement of GABAergic tone, thereby suppressing gonadotropin secretion during the luteal phase.[58] Gene regulation drives the synthesis of key hormones and adapts the cycle to physiological demands. The CYP19A1 gene, encoding the aromatase enzyme, is transcriptionally activated in ovarian granulosa cells by FSH-induced cAMP signaling via phosphorylation of transcription factors such as CREB, converting androgens to estrogens essential for follicular maturation.[59] Epigenetic mechanisms, including DNA methylation and histone modifications, further regulate reproductive gene expression during seasonal anestrus in mammals, silencing pathways like GnRH signaling in response to prolonged melatonin exposure under short photoperiods.[60] Recent investigations have highlighted novel molecular players in cycle dynamics. Melatonin, secreted by the pineal gland in response to photoperiod, modulates estrous onset in seasonal breeders through 2021 studies showing its binding to MT1/MT2 receptors in the hypothalamus, which alters epigenetic marks on clock genes like PER2 to synchronize reproductive timing. Additionally, microRNAs (miRNAs) contribute to luteolysis by post-transcriptionally repressing genes in the prostaglandin pathway. Disruptions in these mechanisms can precipitate reproductive pathologies. Inactivating mutations in the FSH receptor (FSHR) gene, such as those impairing G-protein coupling, disrupt follicular recruitment and estrogen production, resulting in irregular estrous cycles and infertility in female mammals, as observed in murine models with homozygous variants.[61]Variability and Influences
Cycle Length and Frequency
The estrous cycle length refers to the interval from the onset of one estrus to the onset of the next, typically encompassing the full sequence of phases driven by hormonal fluctuations and ovulation. In mammals, this duration varies widely across species, reflecting adaptations to reproductive strategies, with measurements often derived from behavioral observations, vaginal cytology, or hormonal assays in veterinary and research settings. For instance, the cycle is influenced by the rate of follicular development and corpus luteum maintenance, leading to consistent patterns in healthy adults but potential irregularities in other conditions.[37] Mammals exhibit different frequencies of estrous cycles based on annual reproductive patterns. Polyestrous species, such as cattle and pigs, experience continuous cycles throughout the year without prolonged anestrus, allowing multiple breeding opportunities. Seasonally polyestrous animals, like sheep and horses, restrict cycles to specific breeding seasons, typically aligned with environmental optima for offspring survival. Monoestrous mammals, including dogs and certain wild species like foxes, have only one or two cycles per year, with extended intervals between events to concentrate reproductive efforts. These classifications are determined by the number of cycles annually and the presence of anestrous periods.[62] Cycle consistency can be affected by internal factors such as age and health status. In younger animals, cycles may be shorter or more irregular due to immature hypothalamic-pituitary-ovarian axis development, as observed in peripubertal rodents and livestock where initial cycles often deviate from adult norms. Advancing age in adults can lead to progressively shortened cycles or increased variability, linked to declining ovarian reserve and altered hormone dynamics, particularly in mice and primates. Health conditions, including nutritional deficiencies or systemic diseases, further disrupt cycle regularity by impacting hormone production or follicular health, resulting in prolonged or skipped cycles. These influences underscore the importance of monitoring for reproductive management in veterinary practice.[63][64]| Species | Cycle Length (Average and Range) | Frequency Type |
|---|---|---|
| Rat | 4–5 days | Polyestrous |
| Mouse | 4–5 days | Polyestrous |
| Pig | 21 days (19–23 days) | Polyestrous |
| Cattle | 21 days (18–24 days) | Polyestrous |
| Sheep | 17 days (14–19 days) | Seasonally polyestrous |
| Horse | 21 days (19–22 days) | Seasonally polyestrous |
| Dog | 7 months (4–12 months) | Monoestrous (2 cycles/year) |