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Florigen

Florigen is a proteinaceous hormone that serves as the universal systemic signal for inducing flowering in plants, promoting the transition from vegetative to reproductive development in the shoot apical meristem.^[1] The concept of florigen emerged from grafting experiments conducted by Mikhail Chailakhyan in the 1930s, which demonstrated that a transmissible substance produced in leaves under inductive photoperiods could trigger flowering in distant meristems of photoperiod-sensitive plants.^[1] This hypothetical "flower-inducing hormone" was predicted to be synthesized in the leaves and transported via the phloem to the shoot apex, where it activates floral identity genes.^[1] Over decades, extensive genetic and physiological studies in model species like Arabidopsis thaliana and rice confirmed its mobile nature, distinguishing it from other plant hormones like gibberellins.^[2] The molecular identity of florigen was elucidated in 2007, when it was identified as the protein product of the FLOWERING LOCUS T (FT) gene in Arabidopsis and its ortholog Heading date 3a (Hd3a) in rice, marking a pivotal breakthrough in plant developmental biology. These globular proteins, belonging to the PEBP family, are produced in phloem companion cells of leaves exposed to favorable environmental cues, such as long-day photoperiods, and move long-distance to form the florigen activation complex (FAC) in the meristem.^[2] In the FAC, florigen binds to 14-3-3 chaperone proteins and the bZIP transcription factor FD (or OsFD1 in rice), enabling nuclear translocation and direct activation of floral meristem identity genes like APETALA1 (AP1) and LEAFY (LFY).^[3] Beyond flowering induction, florigen functions as a broader growth coordinator, attenuating vegetative growth, simplifying leaf architecture, and accelerating secondary cell wall biogenesis in vascular tissues to align reproductive maturation with structural adaptations.^[4] It is antagonized by anti-florigen proteins like TERMINAL FLOWER 1 (TFL1), which maintain vegetative indeterminacy, creating a balance that shapes plant architecture and yield in crops such as tomato and soybean.^[1] This conserved mechanism across angiosperms has profound implications for agriculture, enabling targeted breeding for flowering time and productivity under varying climates.^[1]

Discovery and Historical Context

Early Hypotheses and Grafting Experiments

The concept of florigen emerged from early 20th-century studies on photoperiodism, the response of plants to day length that triggers flowering. This hypothesis built on the 1920 discovery of photoperiodism by Garner and Allard. In 1936, Soviet plant physiologist Mikhail Chailakhyan proposed that a hormonal signal, produced in leaves under inductive photoperiods, is transmitted to the shoot apex to induce flowering, based on observations of short-day plants such as Perilla and Xanthium.^[5] This hypothesis built on prior work showing that floral induction requires only brief exposure to appropriate light conditions, after which the stimulus persists even if conditions change.^[5] Chailakhyan formalized the term "florigen" in 1936, describing it as a universal flowering hormone synthesized in leaves and mobile throughout the plant, applicable to both short-day and long-day species.^[5] Key evidence came from his grafting experiments conducted between 1936 and 1937, where leaves from photoperiodically induced donor plants were grafted onto non-induced receptor plants maintained under non-inductive conditions; the receptors flowered, demonstrating the transmissibility of the stimulus across the graft union without direct light exposure to the apex.^[5] These results with Perilla and Xanthium indicated that florigen acts independently of the photoperiod at the receptor site, supporting its role as a diffusible signal.^[5] In the late 1930s, American researchers Karl C. Hamner and James Bonner replicated and extended these findings using Xanthium, confirming the long-distance transmission of the floral stimulus through grafting under controlled photoperiods.^[5] Their work, building on initial photoperiodism studies from 1938, validated florigen's mobility and universality across species.^[6] Early debates centered on whether florigen represented a single hormone or a multi-component signal, with some proposing alternatives like the "anthesins" theory involving separate stimuli for different floral aspects, though Chailakhyan's initial view emphasized a unified hormone distinct from known growth regulators like auxin.^[5]

Molecular Identification and Key Milestones

The identification of florigen at the molecular level marked a pivotal shift from conceptual hypothesis to biochemical reality, beginning with genetic screens in the model plant Arabidopsis thaliana during the late 1980s and 1990s. Early mutant analyses revealed that loss-of-function mutations in the FLOWERING LOCUS T (FT) gene caused significant delays in flowering under long-day conditions, positioning FT as a central promoter of the photoperiodic pathway. These ft mutants, alongside complementary studies on the upstream regulator CONSTANS (CO)—first described in 1991 through mutant screening—established a genetic framework linking light perception to floral induction. The CO gene, cloned in 1995, encodes a zinc-finger transcription factor that activates FT expression in response to day length, providing the first molecular handle on the photoperiodic signal. The FT gene itself was cloned in 1999 through activation-tagging experiments that demonstrated its overexpression accelerates flowering, confirming its role as a key integrator of environmental cues. An initial proposal in 2005 suggested that FT mRNA served as the mobile florigen signal based on grafting and expression studies, but this was later retracted in light of evidence favoring the protein form. Breakthroughs in 2007 solidified the molecular identity of florigen: Corbesier et al. demonstrated in Arabidopsis that the FT protein, but not its mRNA, moves from leaves to the shoot apical meristem to induce flowering, using fluorescently tagged constructs to track its mobility. Concurrently, Tamaki et al. showed in rice that the FT ortholog Heading date 3a (Hd3a) protein acts similarly as a graft-transmissible signal, establishing florigen's proteinaceous nature across species. Subsequent research in the 2010s confirmed the conservation of FT-like proteins as florigen equivalents in diverse plants. In tomato, the SELF-PRUNING (SFT) gene, an FT homolog, was identified in 2001 as essential for floral determination, with grafting experiments in the 2010s showing that SFT protein restores flowering in mutants, mirroring FT function. Similarly, Hd3a in rice and other FT paralogs, such as Tomato FT orthologs, were validated through protein-specific grafting assays that induced flowering across genetic barriers, underscoring evolutionary conservation. These proteins also emerged in non-photoperiodic pathways; for instance, FT integrates vernalization signals following the repression of the floral repressor FLOWERING LOCUS C (FLC) by prolonged cold exposure, as shown in studies linking FT activation to winter-adapted flowering. Key milestones culminated in broader recognition of these discoveries' impact. The 1999 FT cloning and 2007 protein mobility confirmations built directly on foundational grafting experiments from earlier decades, transforming florigen from an elusive universal signal into a well-defined family of mobile proteins. The influence of this work was highlighted indirectly in the 2017 Nobel Prize in Physiology or Medicine, awarded for discoveries on molecular mechanisms controlling circadian rhythms, which underpin CO-FT regulation in photoperiodic plants.

Molecular Identity and Function

Definition as a Protein Signal

Florigen is defined as the protein product of the FLOWERING LOCUS T (FT) gene, a member of the phosphatidylethanolamine-binding protein (PEBP) family that functions as the universal flowering signal in angiosperms.^[2] This small globular protein, approximately 20 kDa in size, belongs to the phosphatidylethanolamine-binding protein (PEBP) family and is synthesized specifically in the companion cells of leaf phloem under inductive photoperiodic conditions.^[2] Unlike classic plant hormones such as auxins or gibberellins, which are small diffusible molecules, florigen operates as a phloem-mobile protein signal that travels long distances to induce the transition from vegetative to reproductive growth. The core function of the FT protein lies in its ability to alter the identity of the shoot apical meristem (SAM), promoting the formation of floral primordia and thereby initiating flowering across diverse plant species.^[2] In Arabidopsis thaliana, the model organism where FT was first characterized as florigen, the protein interacts with 14-3-3 chaperones and the bZIP transcription factor FD to form the florigen activation complex (FAC), which directly regulates floral identity genes. This mechanism underscores florigen's role not as a diffusible hormone but as a precise, targeted signal that ensures coordinated developmental responses to environmental cues.^[2] Florigen's identity is evolutionarily conserved, with FT orthologs such as Hd3a in rice (Oryza sativa) sharing the PEBP domain critical for protein stability, chaperone binding, and efficient phloem transport. This conservation highlights florigen's ancient origin and universal deployment in angiosperms to synchronize reproduction with seasonal changes, as evidenced by functional interchangeability of FT-like proteins across taxa.^[2] Grafting experiments in the 1930s provided early evidence for such mobility, later confirmed by the molecular identification of FT as the transmissible agent.

Role in Photoperiodic Flowering

Florigen serves as the primary systemic signal that mediates the photoperiodic control of flowering, enabling plants to synchronize reproductive development with seasonal changes in day length. In long-day plants, such as Arabidopsis thaliana and wheat (Triticum aestivum), exposure to day lengths exceeding a critical threshold—such as ~16 hours for Arabidopsis thaliana—induces florigen production in the leaves, promoting the floral transition. Conversely, short-day plants, including rice (Oryza sativa) and chrysanthemum (Chrysanthemum morifolium), initiate flowering when days are shorter than their critical length, often less than 12 hours, under which florigen synthesis is activated to trigger reproductive growth.^[7] The endogenous circadian clock plays a crucial role in timing florigen production to align with inductive photoperiods, ensuring precise environmental responsiveness. Under long-day conditions in long-day plants, florigen levels peak in the late afternoon or evening, coinciding with the overlap of light perception and internal rhythms, which maximizes signal accumulation for effective floral induction. In short-day plants, similar clock-mediated synchronization occurs during the extended night periods of short days, with florigen expression peaking post-inductive dusk to facilitate seasonal flowering.^[8]^[9] Beyond strict photoperiodism, florigen functions in day-neutral plants like tomato (Solanum lycopersicum), where it promotes flowering through autonomous developmental pathways independent of day length variations. Florigen also integrates with other seasonal cues, such as vernalization in Arabidopsis, where prolonged cold exposure derepresses florigen activity to enable flowering after winter, thus combining multiple environmental inputs for robust timing control.^[10] Quantitative models of photoperiodic flowering emphasize that a threshold level of florigen accumulation is required to initiate the reproductive transition, with experimental evidence from FT overexpression in tobacco (Nicotiana tabacum) demonstrating accelerated flowering once this threshold is met.^[11]

Mechanism of Action

Initiation in Leaves

Florigen production initiates in the leaf vascular tissues of plants, particularly in long-day species like Arabidopsis thaliana, where environmental and genetic cues converge to activate the transcription of the FLOWERING LOCUS T (FT) gene, encoding the florigen protein. The process is tightly regulated by the circadian clock, which ensures that FT expression aligns with photoperiodic conditions favorable for flowering. Under inductive long-day conditions, the transcription factor CONSTANS (CO) plays a central role, with its mRNA accumulating to peak levels approximately 12 hours after dawn, driven by clock-controlled rhythms. This temporal pattern positions CO activity during the late afternoon and evening, when light stabilizes the CO protein. Light-mediated stabilization of CO is mediated by phytochromes, particularly phytochrome B, which prevents CO degradation by the COP1 ubiquitin ligase, allowing CO to accumulate and directly bind to the FT promoter to activate its transcription. Upstream of CO, the GIGANTEA (GI) protein, which oscillates with a circadian rhythm peaking in the evening, contributes to this regulation by stabilizing CO protein levels and facilitating its activity. Mutations in GI, such as gi-3, result in delayed flowering under long days due to reduced CO stability and consequent low FT expression. FT expression is specifically localized to phloem companion cells within the leaf veins, rather than sieve elements, ensuring targeted synthesis in vascular tissues competent for signal production. A 2025 study revealed that companion cells with high FT expression form a distinct subpopulation, marked by unique gene signatures including other small secreted proteins, which may support florigen's specialized role in these cells. Under inductive photoperiods, FT participates in a feedback loop, autoregulating its own transcription to fine-tune production levels and prevent overaccumulation that could disrupt flowering timing.

Translocation via Phloem

The FT protein, identified as the primary component of florigen, is synthesized in the phloem companion cells of leaves and exported into the sieve tubes for long-distance transport to the shoot apical meristem. This export occurs through plasmodesmata, specialized channels that connect companion cells to sieve elements, allowing the 20-kDa FT protein to enter the phloem stream without requiring mRNA mobility. Grafting experiments in Arabidopsis demonstrate that FT protein, but not its mRNA, moves systemically as part of the assimilate stream, with detectable translocation from donor scions to recipient apices within 24 hours under inductive long-day conditions. The gating of FT export is tightly regulated to ensure efficient entry into the phloem. The protein FT-INTERACTING PROTEIN 1 (FTIP1) binds directly to FT in companion cells, facilitating its passage through plasmodesmata into sieve elements while preventing premature degradation. Additionally, the MCTP-SNARE complex mediates endosomal trafficking within companion cells, which is essential for loading FT into the phloem for stable transport. These interactions enhance FT stability during transit, as FT associates with phloem-specific proteins and lipids to avoid proteolysis in the assimilate flow. In Arabidopsis, FT translocation is effective over short distances of 10-20 cm, corresponding to the typical plant height, with phloem sap velocities enabling arrival at the apex within hours to days under optimal photoperiods. In larger plants such as trees, the same mechanism operates over meters, but the longer distances result in slower overall transit times despite comparable phloem flow rates of approximately 0.5-1 cm per minute. Recent studies have identified FPF1-LIKE PROTEIN 1 (FLP1), a small mobile signal co-expressed with FT in companion cells, which accelerates FT-related export processes and promotes stem elongation independently of FT by inducing SEPALLATA3 expression at the meristem.

Activation at Shoot Apical Meristem

Upon arrival at the shoot apical meristem (SAM) via phloem translocation from the leaves, the florigen protein FLOWERING LOCUS T (FT) initiates the floral transition by forming a nuclear protein complex. This process reprograms the vegetative SAM into an inflorescence meristem, shifting its developmental fate from leaf production to flower formation. The core of this activation is the Florigen Activation Complex (FAC), in which FT binds to the bZIP transcription factor FD and the chaperone 14-3-3 protein within the nucleus of SAM cells. The 14-3-3 chaperone stabilizes the interaction between FT and FD, enabling the complex to function as a transcriptional activator. Formation of the FAC exhibits cooperative binding dynamics, allowing sensitive response to varying FT levels and ensuring robust activation at appropriate developmental stages. A 2025 study revealed multifaceted assembly of the FAC, where DNA-bound FD–14-3-3 recruits FT through its C-terminal tail interacting with DNA, and 14-3-3 binding to phosphorylated FD prevents condensation while enhancing DNA binding and dimerization; DNA interaction is essential for efficient FT recruitment. The FAC directly upregulates floral meristem identity genes, including APETALA1 (AP1), SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1), and LEAFY (LFY), which collectively drive the commitment to flowering. A threshold level of FT accumulation in the SAM is necessary to achieve sufficient FAC assembly and trigger this gene activation, beyond which the meristem undergoes irreversible floral determination. This mechanism is conserved across species; in rice, the FT ortholog Heading date 3a (Hd3a) forms an analogous Hd3a-FD-14-3-3 complex that upregulates the AP1 homolog OsMADS15 to induce panicle formation in the SAM.

Counteracting Factors: Antiflorigen

In plants, antiflorigen molecules counteract the flower-promoting effects of florigen to precisely regulate the timing and architecture of flowering. A prominent example is TERMINAL FLOWER 1 (TFL1) in Arabidopsis thaliana, a homolog of the florigen gene FLOWERING LOCUS T (FT) that functions as an antiflorigen by repressing floral transition and maintaining inflorescence indeterminacy. TFL1 achieves this antagonism by competing with FT for binding to the bZIP transcription factor FD and 14-3-3 chaperone proteins at the shoot apical meristem, thereby sequestering these partners and preventing formation of the florigen activation complex (FAC) required for floral gene expression. This inhibitory role ensures that meristems remain vegetative under non-inductive conditions, delaying flowering until appropriate environmental cues accumulate sufficient FT. Similar antiflorigen mechanisms operate in crop species to modulate growth habits. In tomato (Solanum lycopersicum), the SELF-PRUNING (SP) gene, an ortholog of TFL1, acts as an antiflorigen that represses flowering and promotes continuous sympodial shoot growth, resulting in an indeterminate bushy architecture when active. Mutations in SP, such as the classic sp allele, reduce this repression and induce earlier flowering with determinate growth, a trait selected during domestication to facilitate harvest. In perennial plants, homologs like RICE CENTRORADIALIS (RCN) in rice (Oryza sativa) and related species exemplify antiflorigen function by sustaining vegetative meristem activity and inhibiting premature reproductive transitions, allowing prolonged biomass accumulation before flowering. These proteins similarly interact with 14-3-3 and FD-like factors to block floral activation, supporting perennial life cycles with extended vegetative phases. The interplay between florigen and antiflorigen follows a balance model where their relative levels dictate meristem fate: high antiflorigen concentrations, such as elevated TFL1, favor branching and vegetative proliferation over floral determinacy, while dominance of florigen shifts meristems toward reproductive development. A 2025 study showed that florigen and antiflorigen (PEBP family) gene expression correlates with flowering phenotypes across angiosperms, reinforcing the ratio-based regulation in response to environmental signals. This ratio-based regulation fine-tunes plant architecture in response to developmental and environmental signals, optimizing resource allocation for survival and reproduction. Evolutionary studies highlight how antiflorigen genes like TFL1 and SP diversified through gene duplication and regulatory changes, enabling adaptive variation in flowering strategies across species, as detailed in analyses of domestication impacts on crop productivity. Recent advances (2020–2025) have further elucidated FT/TFL1 family evolution, including copy number variations and clade-specific diversification.

Regulation and Triggers

Genetic Factors and Gene Networks

The core of the florigen gene regulatory network in Arabidopsis thaliana centers on the CONSTANS (CO)-FLOWERING LOCUS T (FT) module, where CO acts as a transcriptional activator of FT under inductive photoperiod conditions. CO protein binds directly to the FT promoter through its CCT domain, recruiting nuclear factor-Y (NF-Y) transcription factors to enhance FT transcription and thereby promote the production of the FT florigen protein. This activation is tightly coordinated with the circadian clock, ensuring that FT expression peaks in the late afternoon of long days to facilitate timely flowering. Seminal studies have established that disruptions in CO lead to delayed flowering, underscoring its pivotal role in integrating temporal signals for florigen synthesis.^[12] The CO-FT module is regulated by the evening complex (EC) of the circadian clock, comprising EARLY FLOWERING 3 (ELF3), ELF4, and LUX ARRHYTHMO (LUX), which represses CO expression during the evening to prevent premature florigen accumulation in non-inductive conditions. ELF3 scaffolds the EC, enabling LUX to bind evening elements in the CO promoter and inhibit its transcription, while ELF4 stabilizes the complex; mutations in any EC component result in ectopic CO expression and early flowering. This repression is relieved in long days when CO mRNA accumulates earlier, allowing coincidence with light stabilization of CO protein. The EC thus fine-tunes the network by linking circadian oscillations to photoperiodic output. Upstream regulators from the autonomous pathway, such as FCA and FY, promote florigen signaling by repressing the floral inhibitor FLOWERING LOCUS C (FLC), which otherwise binds to the FT locus to block its activation. FCA, an RNA-binding protein, interacts with FY, a polyadenylation factor, to control alternative polyadenylation of FLC pre-mRNA, reducing FLC levels and derepressing FT in non-vernalized plants. In vernalization pathways, prolonged cold represses FLC to enable FT induction in Arabidopsis; analogously, in cereals, VRN1 (a MADS-box activator induced by vernalization, similar in function to APETALA1) represses the VRN2 repressor (a zinc-finger protein) to promote FT-like genes such as VRN3 (FT ortholog), though these genes are not direct homologs of FLC or Arabidopsis VRN1.^[13] These pathways ensure baseline florigen competence independent of daily light cues. Downstream of FT, the network integrates with the ABC model of floral organ identity, where FT protein at the shoot apical meristem complexes with FLOWERING LOCUS D (FD) and 14-3-3 proteins to activate meristem identity genes like APETALA1 (AP1). AP1, in turn, provides positive feedback by directly upregulating FT expression, reinforcing floral commitment and preventing reversion to vegetative growth. This integration ensures that florigen not only triggers the floral transition but also sustains the genetic program for flower development. The GI-CO-FT components form an oscillatory feedback loop with a approximately 24-hour period, driven by circadian regulation and measurable via luciferase reporter assays. GIGANTEA (GI) promotes CO stability by facilitating the degradation of CO repressors like CYCLING DOF FACTORS (CDFs), while CO and FT expression rhythms align with clock outputs; reporter lines show FT::LUC oscillations peaking under long days, with period lengths varying slightly due to natural allelic diversity. Photoperiod acts as an external synchronizer for this endogenous loop, optimizing florigen timing for environmental adaptation.^[14]

Environmental Cues: Light and Temperature

Florigen production and activity are profoundly influenced by light quality and intensity through photoreceptor-mediated pathways. Phytochrome B (phyB), a red/far-red light photoreceptor, destabilizes the CONSTANS (CO) transcription factor, thereby inhibiting flowering; in contrast, phytochrome A (phyA) and cryptochrome photoreceptors stabilize CO under long-day conditions to promote the expression of the florigen gene FLOWERING LOCUS T (FT) in Arabidopsis thaliana. Cryptochrome photoreceptors, which sense blue light, interact with CO to enhance its activity, further integrating light signals to fine-tune florigen levels in response to daylight spectra. These mechanisms ensure that florigen signaling aligns with optimal photoperiodic windows for flowering induction.^[15] Temperature serves as a critical modulator of florigen, often overriding or synergizing with light cues via distinct genetic repressors and activators. The ambient temperature pathway involves SHORT VEGETATIVE PHASE (SVP), a MADS-box transcription factor that represses FT expression at lower temperatures (typically around 16°C), delaying flowering to prevent suboptimal reproductive timing.^[16] However, elevated temperatures (above 27°C) can induce FT transcription even in short-day plants like rice, bypassing typical photoperiod requirements and accelerating flowering through thermoresponsive chromatin modifications at the FT locus. Recent studies indicate that low ambient temperatures also inhibit long-distance translocation of florigen, further delaying flowering.^[17] This temperature sensitivity highlights florigen's role in adapting to climate variability, with high temperatures promoting earlier bolting in long-day species. The integration of light and temperature signals converges on florigen regulation through secondary messengers such as calcium and calmodulin. Photoreceptors trigger rapid calcium fluxes that activate calmodulin-dependent pathways, leading to the transcriptional upregulation of GIGANTEA (GI) and FT promoters in leaf companion cells. Circadian gene networks act as intermediaries in this process, synchronizing environmental inputs to rhythmic florigen output. Additionally, other environmental cues like gibberellins enhance FT stability and translocation, amplifying florigen signals during stress or growth transitions,^[18] while shade avoidance responses mediated by PHYTOCHROME INTERACTING FACTORS (PIFs) transiently repress florigen to prioritize vegetative elongation under low red-to-far-red light ratios.^[19]

Recent Advances and Applications

Discoveries in Temperature Responses and Florigen-Like Genes

A 2024 review highlighted florigen's pivotal role in mediating the vegetative-to-reproductive phase transition in response to varying temperatures, emphasizing how temperature modulates florigen transport and activity at the shoot apical meristem (SAM) through interactions with membrane lipids like phosphatidylglycerol, which anchor florigen to chloroplasts at lower temperatures to delay flowering.^[20] This mechanism integrates thermal cues with photoperiodic signals, ensuring adaptive flowering timing across species such as Arabidopsis and rice, where florigen forms activation complexes to alter epigenetic states and developmental patterns in the SAM.^[20] In 2025, research identified FTL2, a florigen-like gene in tomato, as a key repressor induced specifically by high temperatures (32°C) in the shoot apex under short-day conditions, where it binds to the promoter of the primary florigen gene SFT to suppress transcription and delay the SAM transition from vegetative to floral identity by up to 13 days.^[21] Mutations in FTL2 accelerated flowering under these conditions, while ectopic expression of SFT in ftl2 mutants restored temperature-insensitive early flowering, underscoring FTL2's role in fine-tuning thermal-photoperiodic integration for reproductive success in short-day crops.^[21] This builds on classical FT signaling by revealing paralogous florigen-like genes as thermal sensors in the apex. Companion cell studies from 2025 revealed that a subpopulation of phloem companion cells with high florigen (FT) expression in Arabidopsis cotyledons and leaves co-expresses unique small proteins, such as BFT (an anti-florigen), which confer resilience to stresses like salinity by modulating FT activity under nutrient fluctuations.^[22] Single-nucleus RNA sequencing of over 4,800 vascular nuclei identified these FT-high cells as a distinct cluster enriched in ATP biosynthesis genes and FT repressors like NIGT1, which respond to high nitrogen by binding the FT promoter to delay flowering, thus linking vascular signaling to environmental stress adaptation.^[22] In saffron, 2025 investigations into florigen activation complex (FAC) dynamics demonstrated that the florigen CsatFT3 competes with the repressor CsatTFL1-3 under heat stress, where SVP-mediated repression of CsatFT3 in the SAM delays flowering at low temperatures, but overexpression of CsatFT3 overrides this to promote timely reproductive transition in this short-day geophyte.^[23] This competitive interaction within the FAC highlights a conserved mechanism for heat-regulated flowering, distinct from classical FT baselines, and offers insights into thermal adaptation in bulbous crops.^[23] A November 2025 study further advanced understanding of FAC dynamics by detailing the multifaceted assembly in the Arabidopsis shoot apical meristem, revealing specific protein interfaces that stabilize interactions between FT, 14-3-3 chaperones, and FD, enabling efficient nuclear translocation and floral gene activation under diverse environmental conditions.^[24]

Implications for Crop Breeding and Biotechnology

The discovery of florigen has revolutionized crop breeding by enabling precise manipulation of flowering time to optimize yield and adapt to changing climates. Overexpression of FT homologs, such as OsFTL10 in rice, accelerates flowering by up to two weeks through upregulation of downstream genes like OsMADS15, allowing for shorter growth cycles and multiple harvests in a single season.^[25] In tomato, virus-induced overexpression of Arabidopsis FT using Potato virus X vectors doubles flower numbers and fruit yield compared to controls, demonstrating practical gains in productivity without altering fruit quality.^[26] These transgenic strategies have been particularly effective in staple crops, where early flowering extends the reproductive phase and enhances overall biomass allocation to seeds. CRISPR/Cas9 editing of CO and FT genes has further advanced the development of climate-resilient varieties by fine-tuning flowering responses to environmental stresses. For instance, targeted mutations in FT homologs modulate flowering time in crops like rice and wheat, enabling adaptation to varying photoperiods and reducing sensitivity to suboptimal conditions.^[27] In tomato, modulation of florigen-like genes such as FTL2, which is upregulated under high temperatures, promotes heat-tolerant flowering, ensuring reproductive success during heatwaves that would otherwise delay or abort blooms.^[28] This approach has yielded varieties with stable heading dates under fluctuating temperatures, minimizing yield losses in field trials. In perennial crops like fruit trees, suppressing antiflorigen genes such as TFL1 homologs promotes continuous blooming and reduces the juvenile phase, accelerating orchard productivity. Antisense expression of MdTFL1 in apple induces early flowering, bypassing the typical multi-year wait for fruit production and enabling faster breeding cycles.^[29] Similarly, knockdown of TFL1-like genes in pear and citrus results in precocious flowering and indeterminate growth habits, supporting higher annual yields in woody perennials.^[30]^[31] Recent applications in 2025 highlight florigen's role in niche crops like saffron, where the florigen activation complex regulates temperature-responsive flowering to boost stigma productivity, a key yield determinant amid climate variability.^[32] Building briefly on advances in temperature-responsive florigen-like genes, canalization mechanisms involving FT signaling ensure stable flower production across thermal gradients, as seen in model systems where florigen synergizes with CLAVATA pathways to buffer against heat stress and maintain consistent yields.^[33] These biotechnological tools collectively promise enhanced food security by tailoring flowering to regional challenges.

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TFL1-Like Proteins in Rice Antagonize Rice FT ... - Oxford Academic
In rice, it has been shown that the FT–FD interaction is mediated by 14-3-3 (Taoka et al. ... florigen activation complex (FAC) (Taoka et al. 2011). The amino ...
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Photoperiodic Regulation of Florigen Function in Arabidopsis thaliana
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Newly Discovered Alleles of the Tomato Antiflorigen Gene SELF ...
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Regulation of Flowering Time by Environmental Factors in Plants
Induction of Flowering by Florigen in Plants. The molecular mechanisms governing flowering time have undergone extensive investigation in Arabidopsis plants.<|separator|>
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The FveFT2 florigen/FveTFL1 antiflorigen balance is critical for the ...
Jun 15, 2021 · The FveFT2 florigen/FveTFL1 antiflorigen balance is critical for the control of seasonal flowering in strawberry while FveFT3 modulates axillary meristem fate ...Missing: RCN | Show results with:RCN
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Revolutions in agriculture chart a course for targeted breeding of old ...
Two hormone systems that universally control flowering and plant architecture, florigen and gibberellin, were the source of multiple revolutions that modified ...
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https://doi.org/10.1126/science.1115983
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Natural variation in transcriptional rhythms modulates photoperiodic ...
To understand how the circadian clock increases plant fitness to local environments, it is important to investigate how natural variation in the clock modulates ...
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The Function of Florigen in the Vegetative-to-Reproductive Phase ...
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Florigen and Florigen‐Like Genes Regulate Temperature ...
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Overexpression of OsFTL10 induces early flowering and improves ...
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Virus-induced overexpression of heterologous FLOWERING LOCUS ...
Potato virus X-induced overexpression of Arabidopsis FLOWERING LOCUS T (FT ) ... PVX induced FT expression increased flower numbers and fruit yield in tomato.
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Gene editing applications to modulate crop flowering time and seed ...
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Antisense Expression of Apple TFL1-like Gene (MdTFL1) Promotes ...
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Florigen Activation Complex Dynamics and SVP‐Mediated ...
Oct 13, 2025 · Flowering is the key determinant of saffron's crop productivity, and this critical phase is increasingly influenced by environmental changes.
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Canalization of flower production across thermal environments ...
Jul 21, 2025 · Elevated florigen bypasses the loss of CLAVATA signaling during flower outgrowth · Heat-induced florigen restores flower formation to crn plants.Missing: review | Show results with:review