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Humulus lupulus

Humulus lupulus, commonly known as or common hop, is a dioecious, rhizomatous, twining in the family, native to temperate regions of and widely naturalized in . The plant features rough, green stems that can reach heights of 15 to 25 feet, opposite leaves that are 3- to 5-lobed with toothed margins, and yellow-green flowers: male in loose catkins and female forming cone-like strobiles that contain resinous lupulin glands. It thrives in moist, rich, well-drained soils in full sun to partial shade, often along streambanks, woodlands, and disturbed areas, and dies back in winter before regrowing from roots in spring. Primarily cultivated for its female cones, harvested in late summer to early autumn, H. lupulus plays a central role in the global industry, where the α-acids (humulones) and essential oils in the lupulin provide bitterness, aroma, and properties to balance sweetness and preserve the beverage. Varieties are selected for seedless cones to optimize yield and quality, with major production in regions like , the , and under temperate climates between 35° and 55° N latitude. Beyond brewing, the plant has ornamental value in gardens for covering arbors and trellises, attracting pollinators like , and historical uses in for and effects due to compounds like .

Description

Morphology and growth

Humulus lupulus is a herbaceous climbing in the , characterized by its vigorous as a that can reach heights of 6 to 10 meters in a single season. It produces annual bines that emerge from an underground rhizomatous , which allows the to overwinter and regrow each . The stems, known as bines, are robust and twine clockwise around supports using hooked trichomes rather than tendrils, enabling rapid vertical of over 30 cm per day under optimal conditions. These bines are typically green, pubescent at the nodes with glandular hairs, and lack wings, measuring 100–700 cm in length. The leaves of H. lupulus are opposite or alternate, simple, and palmately lobed, usually with three to five (occasionally up to seven) deep lobes that give them a maple-like appearance. blades are cordate at the base, measure 3–15 cm in length and width, and feature serrated margins; they are green with glandular dots on the surfaces and petioles up to 7 cm long. Adaxial surfaces may be sparsely pubescent, while abaxial ones are more densely so, with over 20 hairs per cm on the midrib and numerous glands between veins. Morphological variations occur across : European types (H. lupulus ssp. lupulus) have shallower lobes and less pubescence, whereas North American wild hops (ssp. lupuloides or neomexicanus) exhibit deeper lobes, more pronounced pubescence, and sometimes elongated or striped leaves. Root systems consist of a that spreads horizontally underground, producing adventitious and new shoots; the crowns can remain productive for many years but are typically pruned annually in to manage vigor. Lateral branches arise from nodes along the bines, bearing the reproductive structures. The plant is dioecious, with flowers on separate individuals; females are generally taller and produce longer laterals with more nodes than males, particularly in the second year of growth, reflecting in height (females up to 4.8 m vs. males 4.2 m at maturity) and shoot density. Male flowers form loose panicles, while female flowers develop into papery, resinous cones (strobili) 1–10 cm long, containing lupulin glands essential for their commercial use. Fruits are small, ovoid achenes enclosed within the cones. Growth is seasonal and photoperiod-sensitive, initiating in early with shoot emergence after cold stratification for rhizomes or seeds. Bines elongate rapidly during long (requiring 14–16 hours of light) but flower in response to shortening days (<15 hours), with cone maturation occurring 30–50 days later in late summer. The dies back to the in autumn, entering during winter; full vigor is achieved in the second year post-planting, with commercial fields trained on trellises to support the weight and facilitate . In wild settings, it thrives in moist, temperate environments between 30° and 50° , climbing shrubs or trees for support.

Chemical composition

_Humulus lupulus, commonly known as , contains a diverse array of bioactive compounds primarily concentrated in the lupulin glands of the cones. The chemical composition varies by , environmental factors, and plant part, but key constituents include bitter acids, essential oils, , and other secondary metabolites. These compounds contribute to the plant's role in , , and , with bitter acids and oils being most abundant in mature cones. Bitter acids, or humulones and lupulones, are prenylated phloroglucinol derivatives that constitute 2-17% of the dry cone weight, with higher concentrations (up to 30%) in the lupulin glands. The α-acids, including (35-50% of total α-acids), cohumulone (20-35%), and adhumulone (10-20%), isomerize during to form iso-α-acids responsible for beer bitterness and foam stability. β-Acids, such as lupulone (30-50% of total β-acids), exhibit properties but are less soluble in beer. These acids' content is genetically determined, with commercial varieties selected for α-acid levels of 8-15%. Essential oils, comprising 0.5-3% of dry cone mass, are terpenoid mixtures that impart aroma to beer and potential therapeutic effects. Monoterpenes like myrcene (8-52% of oil) dominate in varieties such as and Northern Brewer, contributing herbal and citrus notes, while sesquiterpenes including α-humulene (12-51%) and β-caryophyllene (4-11%) prevail in noble hops like Saaz and Hallertauer, providing earthy and spicy profiles. Oxygenated compounds, such as (0.2-3.2%), add floral aromas. Oil composition influences hop variety classification for . Polyphenols, ranging from 3-14% of dry cones, include and acids with and estrogenic activities. Prenylated chalcones like (0.1-1% of cones) and its metabolites, such as isoxanthohumol, show anticancer potential, while flavanols like (0.03-0.3%) and glycosides like contribute to oxidative stability. , a potent , occurs at 25-60 mg/kg in cones. These compounds are more abundant in leaves and early-stage cones. Other constituents include carbohydrates (up to 15% as pectins and sugars), proteins (10-15%), and minor volatiles like aldehydes and esters in aqueous extracts, which vary by and support the plant's nutritional profile.
Compound ClassKey ExamplesTypical Content (% dry cone weight)Primary Function
Bitter Acids, Lupulone2-17%Bitterness,
Essential Oils, α-Humulene0.5-3%Aroma,
Polyphenols, 3-14%, Phytoestrogenic

Taxonomy

Etymology and classification

The scientific name Humulus lupulus was first published by in his in 1753. The genus name Humulus is a Latinized form derived from medieval European languages, with origins likely in "hoppe" or "chmele," both referring to the hop plant itself. Alternative interpretations link it to Latin humus (rich ), reflecting the plant's preference for fertile, humus-rich ground. The specific epithet lupulus is the diminutive of Latin lupus (), a reference noted by the Roman naturalist in his Naturalis Historia, who compared the plant's aggressive climbing and twining habit—strangling supports like osiers—to a wolf attacking a sheep. The common English name "hop" stems from the or Anglo-Saxon hoppan, meaning "to climb," alluding to the species' vigorous vining growth. In modern taxonomic classification, Humulus lupulus belongs to the kingdom Plantae, phylum Tracheophyta, class Magnoliopsida, order Rosales, family Cannabaceae, genus Humulus, and species lupulus. The family Cannabaceae also includes the genus Cannabis, and Humulus is a small genus comprising seven species as of 2025, with H. lupulus (restricted to European origins) being the type species designated by Linnaeus. Historically, the genus was classified within the Urticaceae family and Urticales order, or even the Moraceae (mulberry family) by earlier taxonomists, due to superficial similarities in inflorescence structure; these placements reflected pre-molecular phylogenetic understandings before revisions in the late 20th century integrated it into Rosales based on floral and genetic evidence. The plant is dioecious, with separate male and female individuals, a trait typical of Cannabaceae. Synonyms include Lupulus humulus Mill., though the Linnaean binomial remains the accepted name.

Varieties and cultivars

As of 2025, the former varieties of Humulus lupulus described by Small (1978) based on morphological traits such as leaf pubescence, gland density, and hair characteristics have been elevated to distinct species status by authoritative sources like Plants of the World Online (POWO), reflecting their geographic origins, adaptations, and genetic divergence across the Northern Hemisphere. H. lupulus is now primarily the European species, with leaf blades featuring fewer than 20 hairs per centimeter on the midrib and limited glands between veins, serving as the foundation for many traditional brewing strains. The North American species include H. neomexicanus, distinguished by deeply lobed leaves (often five to seven lobes) and high gland density exceeding 30 per square centimeter, distributed in the southwestern United States and Mexico; H. pubescens, characterized by conspicuous pubescence with over 100 hairs per centimeter on the midrib and spreading hairs on leaf surfaces, found in the midwestern U.S.; and H. americanus (formerly var. lupuloides), with moderate hair density (20-75 per centimeter) and appressed hairs, native to eastern North America and parts of Canada. An additional East Asian species, H. cordifolius, occurs in Japan, Sakhalin, and eastern Asia, noted for its heart-shaped leaves and finer pubescence. Commercial cultivars of Humulus number over 400 worldwide, selectively bred since the 19th century for traits like alpha-acid content, aroma profiles, yield, and disease resistance, predominantly derived from H. lupulus and wild North American species germplasm. European noble hops, such as Saaz (from Czech H. lupulus lineages, prized for spicy, herbal notes with 3-5% alpha acids) and Hallertau Mittelfrüh (German, offering earthy and floral aromas at 3.5-5.5% alpha acids), emphasize subtle bitterness and are staples in lagers. English cultivars like Fuggle (mild, woody, and minty with 4-5.5% alpha acids) and East Kent Goldings (fruity and floral, 5-6% alpha acids) contribute to ales and bitters. American varieties, often hybrids incorporating H. neomexicanus or H. americanus germplasm, include Cascade (citrusy and piney, 5-9% alpha acids, a cornerstone of craft IPAs since the 1970s) and Centennial (floral with lemon and spice, 9-12% alpha acids). High-alpha bittering hops like Columbus (resinous and citrus, 14-18% alpha acids) and Nugget (herbal and earthy, 12-14% alpha acids) dominate large-scale production. The U.S. Department of Agriculture's breeding program has introduced resilient cultivars such as Brewers Gold (high-resin, from Manitoba wild × English crosses) and Northern Brewer (versatile dual-purpose, 8-10% alpha acids), enhancing adaptation to Pacific Northwest climates, where nearly all U.S. hops are grown, accounting for about 35-40% of global production (as of 2023). Ornamental cultivars, like the yellow-leafed 'Aureus', are propagated for garden use rather than commercial harvest.

Phylogenetic relations

Humulus lupulus belongs to the genus Humulus within the family Cannabaceae, a small lineage of flowering plants in the order Rosales. Phylogenetic analyses confirm that the genus Humulus is closely related to Cannabis, with both forming a monophyletic clade in nuclear-based phylogenies derived from extensive genomic datasets. A comprehensive phylogenomic study utilizing 90 nuclear genes and 82 chloroplast loci across 82 species representing all Cannabaceae genera resolved Humulus as sister to Cannabis in the nuclear tree, highlighting shared evolutionary history including conserved sex chromosome systems. However, significant cyto-nuclear discordance exists, as chloroplast phylogenies place the HumulusCannabis clade in varying positions relative to genera like Trema (including Parasponia) and Celtis, attributed to incomplete lineage sorting or historical gene flow. This discordance underscores the complex reticulate evolution within Cannabaceae, where Humulus diverged from a common ancestor with Cannabis approximately 27.8 million years ago based on chloroplast divergence estimates. The genus Humulus includes seven recognized species as of 2025: H. lupulus (common hop, European), H. japonicus (Japanese hop), H. yunnanensis (Yunnan hop, southwestern China), H. neomexicanus (southwestern North America), H. pubescens (midwestern North America), H. americanus (eastern North America), and H. cordifolius (East Asia). Molecular phylogenies of Humulus species, based on nuclear ribosomal DNA (ITS regions including ETS and IGS) and chloroplast DNA sequences, indicate that H. lupulus is distinct from the East Asian H. japonicus, H. yunnanensis, and H. cordifolius, with the East Asian species showing closer affinities to each other. Genetic variation is structured into primary clades corresponding to geographic regions: a European clade for H. lupulus encompassing populations from Europe, the Caucasus, and Altai regions, and an Asian–North American clade including Chinese, Japanese, and North American species. This bifurcation suggests an East Asian origin for the genus, followed by westward migration to Europe and eastward dispersal to North America via Beringian land bridges, with divergence times estimated at 0.08–0.54 million years ago for North American lineages. Population-level phylogenetics reveal high genetic diversity and regional differentiation, particularly among wild accessions. In Europe, amplified fragment length polymorphism (AFLP) and sequence analyses demonstrate overlapping genetic variability across central and western populations of H. lupulus, with closer relationships among them than to Caucasian populations, which form a distinct subgroup possibly reflecting geographic isolation. Wild North American Humulus species exhibit even greater haplotype diversity in both nuclear and chloroplast markers, supporting multiple colonization events from Asian progenitors and ongoing gene flow in some regions. These patterns of phylogenetic structure have implications for conservation and breeding, as wild Humulus species harbor unique alleles absent in cultivated varieties, especially following recent taxonomic elevations that highlight their distinct evolutionary lineages.

Biogeography

Native distribution

Humulus lupulus is native to the temperate regions of the , spanning , , and . The species thrives in diverse habitats within these areas, often along riverbanks, in woodlands, and disturbed sites, reflecting its adaptation to moist, well-drained soils in latitudes between approximately 35° and 55° N. The nominate subspecies, H. l. subsp. lupulus, originates across —from and in the southwest to in the east—and extends into western , including northern and the . This subspecies forms the basis for much of the wild populations in these regions, where it has been documented in countries such as , , , and . In eastern Asia, the variety H. l. var. cordifolius is indigenous, with native occurrences in and parts of continental , such as and the Russian Far East. This variety contributes to the species' genetic diversity in East Asian temperate zones. North American native populations are represented by subspecies such as H. l. subsp. americanus in the eastern United States and , found in riparian areas from to the Midwest, and H. l. subsp. neomexicanus in the western United States, ranging from the to the Southwest. These indigenous forms are widespread across much of the continent, excluding arid deserts and extreme northern territories.

Cultivation and production

Humulus lupulus, commonly known as , is cultivated as a herbaceous climber primarily for its cones used in . The plant thrives in temperate climates between approximately 35° and 55° latitude north, requiring at least 120 frost-free days for optimal growth and flowering, full exposure to enhance oil and aroma production, and cool winters with temperatures below 40°F (4°C) for . It prefers deep, well-drained sandy or soils with 5-7% and a of 6.0-7.0, avoiding heavy clay or poorly drained sites that can lead to . Annual soil testing is essential to maintain fertility levels, including 120-150 pounds of per , moderate , and adequate around 100 ppm, supplemented by micronutrients like and . Commercial cultivation begins with planting disease-free rhizomes or greenhouse-grown transplants in after the last , typically spaced 3-4 feet apart in rows 10-12 feet wide to accommodate machinery. Plants are grown on permanent trellis systems reaching 18-25 feet high, constructed from durable wood or metal posts with or strings for support; each mature sends up 2-3 vigorous bines that climb clockwise and can grow 1-2 feet per day during the rapid summer phase. is critical during peak growth in June-, providing about 6 gallons per daily to support development, while fertility is managed through split applications of and fertilizers from to . Plants reach full production in their second or third year and can remain productive for 25 years or more on suitable sites, though regular , , and disease monitoring—particularly for downy and —are necessary to sustain yields. Harvesting occurs from late to early when cones are mature, papery, and exude sticky yellow lupulin glands, typically lasting 7-10 days per field. Cones are picked by hand or machine (costing $14,000-200,000 for commercial harvesters), yielding 1-1.5 pounds of dried per bine, and immediately dried at 122-140°F (50-60°C) to 8-10% moisture to preserve quality before storage in vacuum-sealed, frozen conditions. Average yields vary by region and variety, with U.S. national averages around 1,981 pounds per , though eastern states like achieve 1,100-1,500 pounds per due to higher disease pressure. Global hop production is concentrated in a few key regions, with total acreage declining to 55,715 hectares in 2024, a 7.7% drop from 2023, reflecting market adjustments and idling of fields. Production volume reached 113,538 metric tons in 2024, down 3.9% from the previous year, with an average alpha acid content of 10.5%—an increase from 10.0% in 2023—yielding 11,912 metric tons of alpha acids overall. The United States and Germany dominate, accounting for roughly equal shares of acreage at 18,513 hectares and 18,962 hectares respectively in 2024; the U.S. produced 87.1 million pounds (39,500 metric tons) in 2024, a 16% decline from 104 million pounds in 2023, primarily from Washington, Oregon, and Idaho. Other major producers include the Czech Republic (4,845 hectares), China, Poland, and Slovenia, with global output historically around 132,000 metric tons annually as of 2023. Aroma varieties comprise about 70% of production, driven by craft brewing demand, while climate challenges like variable weather have prompted adaptations such as variety selection and irrigation improvements in emerging areas. Global hop acreage continued to decline in 2025, reaching approximately 52,900 hectares as of June 2025.

Ecology

Habitat preferences

Humulus lupulus, the common hop, naturally inhabits temperate regions across the , including parts of , western , and , where it thrives in diverse settings such as hedgerows, woodlands, sunny waste grounds, and riparian zones. In its native North American range, particularly the upper , wild populations of the variety lupuloides are commonly found along well-drained terraces of river basins, riparian corridors, (Salix spp.) patches, exposed rock outcrops, and upper terraces. In naturalized areas, it can form dense stands that may outcompete native vegetation in disturbed habitats. The plant exhibits broad soil tolerance, growing in light (sandy), medium (loamy), and heavy (clay) soils with a pH range of 4.5 to 8.2, though it prefers deep, rich loams that provide good drainage to prevent root rot. It favors moist but not waterlogged conditions, showing drought tolerance once established, and is often associated with alluvial soils in floodplain habitats. Ecologically, H. lupulus frequently co-occurs with riparian tree and shrub species such as boxelder (Acer negundo), ash (Fraxinus spp.), cottonwood (Populus spp.), bur oak (Quercus macrocarpa), and various willows, which provide climbing support and shade in partially wooded areas. Climatically, the species is adapted to temperate zones between approximately 35° and 55° N latitude, requiring cool winters for (hardy to -20°C) and warm summers with average temperatures of 16–18°C for optimal . It demands full sun to partial , with ample to vigorous climbing up to 6–8 meters on supports, and requires at least 120 frost-free days annually for optimal cone production and maturation. In semi-arid extensions of its range, such as Mediterranean-influenced areas, it demonstrates resilience to moderate and , though prolonged or temperatures exceeding 40°C can stress populations.

Pests and diseases

Humulus lupulus, commonly known as hops, is susceptible to a range of fungal, viral, and oomycete diseases that can significantly impact yield and quality in cultivation, particularly in humid environments. Downy mildew, caused by the oomycete Pseudoperonospora humuli, is one of the most destructive diseases, leading to stunted shoots, brittle growth, and brown lesions on leaves and cones with yellow halos; it thrives in cool, wet conditions and can cause up to 100% yield loss if unmanaged. Powdery mildew, induced by the fungus Podosphaera macularis, manifests as white powdery lesions on leaves, stems, and cones, often starting as yellow spots, and is exacerbated by reduced airflow and high humidity, potentially reducing cone quality and plant vigor. Other notable fungal diseases include gray mold (Botrytis cinerea), which produces tan to brown lesions on cones with gray fuzzy growth under prolonged moisture, and Verticillium wilt (Verticillium albo-atrum or V. dahliae), characterized by yellowing between leaf veins and brown discoloration in vine interiors, favored by nitrogen-rich soils. Viral infections further threaten hops, primarily transmitted by insect vectors. The hop mosaic virus complex, including hop mosaic virus, hop latent virus, and American hop latent virus, causes yellow-green mottling on leaves and reduced plant growth, with yield losses ranging from 15% to 62% depending on infection severity and variety. Root and crown rots, such as black root rot (Phytophthora citricola) and red crown rot (Phomopsis tuberivora), lead to blackened or discolored roots, wilting foliage, and uneven root development, often in poorly drained soils. Sooty mold, associated with Cladosporium spp. and Fumago spp., appears as black coatings on leaves and cones due to honeydew from aphid infestations, promoting wilting and rapid leaf death. Among insect pests, the hop aphid (Phorodon humuli) is a primary concern, feeding on sap to cause leaf curling, wilting, and cone browning while vectoring viruses; populations peak in cool, wet seasons and can lead to indirect damage through sooty mold. Two-spotted spider mites (Tetranychus urticae) damage leaves by puncturing cells, resulting in light stippling, bronzing, and shriveling, particularly under hot, dry conditions. Potato leafhoppers (Empoasca fabae) inject toxins during feeding, causing V-shaped yellowing and browning at leaf tips with stunted growth, while Japanese beetles (Popillia japonica) skeletonize foliage by consuming leaf tissue between veins. Other pests like the California prionus beetle (Prionus californicus) attack roots, leading to vine wilting and yellowing. Integrated pest management strategies, including resistant cultivars, cultural practices like improving drainage and airflow, and targeted applications of fungicides or insecticides, are essential for controlling these threats, with early scouting recommended to prevent economic losses in commercial hop yards.

Uses and applications

In brewing

_Humulus lupulus, commonly known as hops, plays a central role in beer brewing through its female inflorescences, or cones, which are harvested and processed to impart bitterness, aroma, , and to the final product. These cones contain specialized glandular structures called lupulin glands that secrete resins and essential oils responsible for these attributes. Approximately 98% of global hop production is dedicated to , underscoring its indispensable status in the industry. The primary bittering agents are alpha-acids, such as , which constitute 2–18% of the cone's dry weight depending on the ; during , these isomerize into iso-alpha-acids, contributing 50–60% of the perceived bitterness while also enhancing stability and properties. Beta-acids, like lupulone (2–12% dry weight), provide supplementary bitterness and preservation effects but are less soluble and primarily inhibit , extending beer's shelf life by preventing spoilage. Essential oils, comprising 0.5–3% of the cone mass, deliver aroma and flavor profiles; dominant compounds include (woody, resinous notes), (spicy, herbal), and (floral, citrus), with terpenoids and polyfunctional thiols adding fruity or tropical nuances that can be biotransformed by during . Polyphenols, such as (0.1–1% dry weight), further contribute activity, flavor complexity, and microbial inhibition. In the brewing process, are added at specific stages to optimize their contributions. For bitterness, whole cones, pellets, or extracts are introduced early in the 60–90 minute , where heat isomerizes alpha-acids and extracts bitter resins, with time and influencing yield (typically 25–35% utilization). Late additions during the final 5–15 minutes of boiling or in the capture more volatile aroma compounds with minimal , preserving floral and fruity notes. Dry hopping, where are added post-fermentation during conditioning or lagering, maximizes aroma extraction without bitterness—often achieving peak oil transfer in 2–3 days—though factors like temperature (ideally 10–20°C), agitation, and strain affect volatile retention and . Hop products like Type 90 pellets (compressed, isomerized forms) or reduced isomerized alpha-acid extracts (e.g., tetrahydroiso-alpha-acids) allow precise dosing and improved efficiency, reducing volume needs by up to 50% while enhancing light stability in pale lagers. Hop varieties are bred for specific brewing roles: high-alpha cultivars like Magnum (12–14% alpha-acids) for pure bitterness in international bitters, noble aroma types such as Saaz (3–5% alpha-acids, high humulene) for delicate Pilsners, and dual-purpose American varieties like Cascade (5–9% alpha-acids, elevated myrcene) for citrus-forward IPAs. These selections influence beer styles, with alpha-acid content measured via high-performance liquid chromatography to ensure consistency. Ongoing research explores hop-derived antimicrobials for non-alcoholic beers and genetic modifications to yeast for enhanced hoppy esters, addressing climate challenges and sustainability in production.

Medicinal and other uses

_Humulus lupulus, commonly known as , has been employed in across various cultures for its , , and digestive properties. In medieval , it was used as a remedy for and restlessness, with records of hop pillows aiding sleep as early as the 18th century for figures like King George III. Arabic physicians such as documented its use for blood purification, fever reduction, and inflammation relief, while Native American tribes, including the and , applied it as an and anti-rheumatic agent. These historical applications highlight its role as a versatile herbal remedy, often prepared as teas, tinctures, or poultices. The pharmacological effects of hops are primarily attributed to its bioactive compounds, including bitter acids ( and lupulone), prenylated ( and ), and essential oils ( and ). These components contribute to sedative actions by modulating GABA_A receptors and pathways, promoting relaxation and sleep induction. Additionally, exhibits potent activity, scavenging free radicals and inhibiting , while acts as a , binding to receptors. properties target , with minimum inhibitory concentrations as low as 8-64 µg/mL for α- and β-acids against pathogens like . Anti-inflammatory effects involve suppression of cytokines such as TNF-α and IL-6 through pathway inhibition. In contemporary , hops extracts are utilized in herbal supplements, often combined with , for treating sleep disorders and anxiety, with clinical trials demonstrating reduced sleep latency by approximately 44 minutes in participants. For menopausal symptoms, has shown efficacy in alleviating hot flashes and improving quality-of-life scores, as evidenced by a randomized trial where the Kupperman Menopausal Index decreased significantly (p=0.023). Preclinical studies further support its potential in metabolic disorders, with isohumulones improving glycemic control and reducing body fat in prediabetic individuals, and mitigating and in animal models by activating PPARα/γ receptors. Anticancer research highlights xanthohumol's ability to induce in various lines, including colon and , via ROS-mediated pathways, though human trials remain limited. Regulatory bodies like the Commission E endorse hops for restlessness and sleep disturbances based on these findings. Beyond medicinal applications, hops serve as a source of functional biomolecules in the , , and pharmaceutical industries. Its polyphenols and bitter acids are incorporated into dietary supplements for protection and into for anti-aging formulations due to their free radical scavenging capacity, which is up to 8.9 times that of in hydroxyl radical assays. In the sector, hops-derived compounds enhance product stability against oxidation, while their qualities support natural preservation in beverages and . These non-brewing uses leverage the plant's chemical diversity for innovative product development.

Health and safety

Toxicity

Humulus lupulus, commonly known as , is (GRAS) for human consumption by the U.S. , with essential oils, oleoresins, and natural extractives approved for use in food and beverages. Long-term exposure through and dietary intake has not been associated with significant adverse effects or in humans. Acute oral toxicity studies indicate high LD50 values, such as 3,500 mg/kg in mice and 2,700 mg/kg in rats for ethanolic extracts, suggesting low risk from ingestion at typical doses. However, potential side effects from herbal supplements or extracts include drowsiness, , and rare reactions. Hops have not been linked to or in clinical reports. Occupational exposure to hops can lead to and respiratory issues. Handlers, particularly farmers, may develop allergic airborne due to sesquiterpene lactones in the plant, with symptoms including , , and on the face, neck, and hands. Inhalation of hop dust has been associated with , including asthma-like symptoms, in workers, though rates are low (about 15 cases per 10,000 workers). Cosmetic use of hop extracts is considered safe at concentrations up to 0.6%, with no significant or in repeat insult tests. Weak estrogenic activity from compounds like exists but is far less potent than (10-20,000-fold weaker). In animals, hops pose a severe toxicity risk, particularly to dogs, where ingestion can induce malignant hyperthermia. Symptoms typically onset within 30 minutes to 8 hours and include rapid breathing, elevated heart rate, vomiting, diarrhea, agitation, and body temperatures exceeding 105°F (40.6°C), which can be fatal without intervention. The mechanism is not fully understood but may involve phenolic compounds uncoupling oxidative phosphorylation; dried hop plugs are more toxic than pellets. Certain breeds, such as greyhounds and Labrador retrievers, appear predisposed due to genetic factors related to hyperthermia. Cats are rarely affected, with no confirmed cases reported. Treatment requires immediate veterinary care, including emesis induction, activated charcoal, dantrolene administration (2-3 mg/kg IV), and aggressive cooling measures to reduce temperature below 103.5°F (39.7°C). Prevention involves keeping hops and brewing materials inaccessible to pets.

Pharmacological research

Pharmacological research on Humulus lupulus primarily focuses on its bioactive compounds, including prenylated flavonoids like and , bitter acids such as and lupulone, and essential oils, which exhibit a range of potential therapeutic effects. These compounds have been investigated for their , , , and neuroprotective properties in preclinical models, with emerging evidence from clinical trials suggesting applications in metabolic and hormonal disorders. Research emphasizes the variability in compound concentrations across hop varieties, influenced by cultivation factors, which impacts their pharmacological potency. Sedative and anxiolytic effects have been demonstrated in , where CO₂ hop extracts reduced locomotor activity and prolonged barbiturate-induced in , potentially mediated by interactions with GABA_A receptors and modulation of monoamine neurotransmitters. Clinical evidence remains limited, with one trial showing that a valerian-hops combination improved quality in patients with , though hops' isolated contribution was not delineated. A 2025 study on Hongcheon-hop extract further supported sleep-enhancing effects by improving structure in preclinical models. Neuroprotective potential is supported by findings where iso-α-acids enhanced β-amyloid phagocytosis in Alzheimer's disease mouse models, reducing cognitive decline at doses of 4-20 mg/kg. Anti-inflammatory and antioxidant activities are prominent, with xanthohumol inhibiting pro-inflammatory cytokines like IL-6 and pathways and . In a involving patients, an xanthohumol-enriched extract (1.5 mg/kg three times daily) significantly lowered IL-6, , and neutrophil-to-lymphocyte ratios after seven days. Antioxidant effects include scavenging and hydroxyl radicals by hop polyphenols, with values around 54.90 μg/mL for xanthohumol. Antimicrobial properties are evident against like Bacteroides fragilis and fungi, attributed to bitter acids disrupting microbial membranes. Anticancer research highlights xanthohumol's ability to induce and inhibit in , colon, and cell lines via Akt and modulation, with IC50 values as low as 6.87 μM in MCF-7 cells. A 2025 meta-analysis confirmed the antiproliferative potential of hop-derived compounds across multiple studies. In vivo studies show it accelerates diabetic wound healing through Nrf2 activation in mice. Phytoestrogenic effects of have been explored for menopausal symptoms; a randomized trial found vaginal hop extract as effective as in improving sexual function scores on the Female Sexual Function Index, with no adverse events after two months. Iso-α-acids also demonstrate metabolic benefits, reducing and lipid accumulation in models. Overall, while preclinical data is robust, human trials are sparse and often small-scale, underscoring the need for larger studies to validate efficacy and safety.

Cultural and historical aspects

In culture

Humulus lupulus, commonly known as hops, derives its binomial name from Latin roots reflecting cultural perceptions of its growth habit. The genus Humulus derives from medieval Slavic or Germanic terms related to hops, such as chmele or humel, while lupulus is a diminutive of lupus, or "wolf," based on ancient Roman observations of its aggressive, twining vines that "devour" supporting plants like wolves among willows. This wolf association imbued hops with a symbolic tenacity and wildness in early European herbals. In folklore, hops have been linked to sleep and tranquility, often featured in "dream pillows" stuffed with the dried strobiles to promote rest. Historical accounts claim King George III of Britain (1738–1820) and U.S. President used such pillows to alleviate , embedding the plant in royal and presidential lore as a natural . A legend from 1406 recounts King Wenceslas IV of Bohemia granting brewers the right to incorporate hops cones into their after discovering the rejuvenating effects of brewery sludge baths containing the plant, highlighting its early cultural ties to guilds and prosperity. Hop harvesting traditions in , particularly in and from the 18th to early 20th centuries, incorporated festive rituals suggestive of pre-Christian . During the annual hop-picking season, young men and women were playfully submerged in bins of freshly picked , a custom documented in such as Christopher Smart's 1752 poem "The Hop-Garden" and George Orwell's 1931 diary, symbolizing abundance and communal bonding among migrant workers. In Bavarian beer culture, the "Hopfen und Malz, Gott erhalt's" ("Hops and malt, God preserves") underscores trust in the plant's role for successful , appearing on traditional steins and brewery mottos as a nod to divine favor in . These practices reflect hops' broader of relaxation, economic vitality in brewing regions, and cyclical . In modern times, have become central to culture, inspiring festivals like the and hop harvest events in the , symbolizing innovation and regional pride as of 2025.

Historical significance

Humulus lupulus, known as the common hop, has origins tracing back to the temperate regions of the Northern Hemisphere, with possible early references as a salad plant in ancient texts from the 1st century AD. Archaeological evidence suggests even earlier incidental use, such as hop pollen found in a 6th-century BCE beaker from northern Italy, potentially indicating hopped beverages, though this remains speculative. The plant's historical significance emerged prominently in the early Middle Ages through its adoption in brewing, transforming perishable fermented drinks into more stable products. The first documented use of hops in beer occurred in 822 AD in northern France, where Abbot Adalhard of the Benedictine monastery of Corbie regulated hop cultivation for brewing to ensure supply and quality. By the 9th century, hops spread to Germany, valued for their antimicrobial properties that preserved beer and imparted bitterness, gradually replacing herbal mixtures like gruit. In the 12th century, Hildegard von Bingen documented hops in her Physica, praising their preservative role in beverages while noting their tendency to foster melancholy if overconsumed. This period marked hops' integration into European monastic brewing traditions, enabling wider trade as hopped beer resisted spoilage during transport. Medicinally, hops featured in medieval herbals for sedative and diuretic effects, with 16th-century physicians like recommending them for digestion and Matthiolus highlighting their urinary benefits. Indigenous North American groups, including the and , employed hops traditionally as analgesics and anti-rheumatics. The 1516 Bavarian (purity law) enshrined hops as an essential beer ingredient alongside water and malt, standardizing their use and boosting cultivation across . By the 15th century, hops reached England from Flanders, spurring domestic cultivation in Kent and shifting preferences from unhopped ale to hopped beer, which fueled the Industrial Revolution's brewing industry. In the 19th century, isolation of lupulin in 1813 revealed hops' sedative compounds, leading to applications like hop-stuffed pillows for insomnia, as used by King George III. Overall, Humulus lupulus profoundly influenced European culture by stabilizing beer production, supporting economic growth through hop farming, and contributing to herbal pharmacopeias.

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