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Network motif

Network motifs are small, recurring subgraphs or patterns of interconnections within complex networks that appear at frequencies significantly higher than those in randomized counterparts, functioning as the basic building blocks of network structure and dynamics.^[1] These motifs are identified across diverse domains, including biological systems such as transcription regulatory networks in bacteria like Escherichia coli, neuronal wiring in Caenorhabditis elegans, ecological food webs, and engineered systems like the World Wide Web.^[1] Unlike random wiring, which lacks such overrepresented patterns, network motifs suggest evolutionary or design principles that enhance functionality, such as efficient information processing or robustness.^[1] The identification of network motifs involves computational algorithms that enumerate all possible small subgraphs (typically of size 3 to 7 nodes) in a given network and compare their occurrences to those in an ensemble of randomized networks with preserved degree distributions.^[2] This statistical approach, first applied systematically to biological networks, reveals motifs by calculating Z-scores or P-values to determine overrepresentation.^[2] In practice, tools like those developed for transcription networks detect motifs by sampling randomization iterations to account for network size and sparsity.^[2] In biological contexts, network motifs underpin key regulatory mechanisms, with prominent examples including the feed-forward loop (FFL) and feedback loops.^[2] The coherent FFL, where an operon regulates both a target gene directly and indirectly through an intermediary, acts as a persistence detector to filter noisy or transient signals in gene expression, as seen in the E. coli arabinose system.^[3] In contrast, incoherent FFLs generate rapid pulses or accelerate responses, exemplified by the galactose (gal) system in E. coli.^[2] Feedback motifs, such as negative autoregulation, reduce cell-to-cell variability and hasten response times, while positive autoregulation amplifies signals and promotes bistability.^[2] Beyond transcription, motifs extend to signaling cascades, protein interaction networks, and even non-biological systems, where they facilitate modular design and fault tolerance.^[2] Experimental validation, through synthetic biology and perturbation studies, confirms that rewiring motifs alters network behaviors predictably, underscoring their role in evolutionarily conserved design principles across scales from molecular to ecosystem levels.^[2]

Fundamentals

Definitions

Network motifs are small, recurring subgraphs within a larger network that appear with a frequency significantly higher than expected in randomized networks with similar structural properties, such as degree distribution and edge directionality. These motifs are particularly studied in directed graphs, where edge orientations matter, and are typically of sizes 3 to 7 nodes, with larger sizes possible using advanced algorithms despite computational challenges. In biological and technological networks, such as gene regulatory or electronic circuits, motifs represent fundamental building blocks that capture non-random wiring patterns. The mathematical foundation of network motifs relies on enumerating subgraph isomorphisms, where the count N of a motif m in a network G is the number of injective mappings from the nodes of m to subsets of nodes in G that preserve all edges and non-edges. Significance is assessed by comparing this observed count N_{\text{real}} to the distribution obtained from an ensemble of randomized networks, using the Z-score as a standard measure:

Z = \frac{N_{\text{real}} - \langle N_{\text{rand}} \rangle}{\sigma_{N_{\text{rand}}}}

where \langle N_{\text{rand}} \rangle is the mean count across random networks and \sigma_{N_{\text{rand}}} is the standard deviation. Motifs are identified when Z > 2, indicating overrepresentation beyond statistical fluctuation.^[4] Unlike random subgraphs, which occur at expected frequencies in null models, network motifs exhibit statistical enrichment that suggests functional or structural importance. They also differ from simple graph patterns like cliques (fully connected subgraphs) or stars (central node connected to leaves), as motifs are defined by their overrepresentation rather than a fixed topology, and many such patterns may not qualify as motifs in a given network. Practical analysis of network motifs is constrained by computational complexity, as the number of possible subgraphs grows factorially with node count, making exhaustive enumeration infeasible beyond small sizes even for moderately sized networks.^[4] This limitation arises from the NP-hard nature of subgraph isomorphism, restricting motif studies to small scales where exact counting is viable.^[4]

Significance

Network motifs serve as fundamental building blocks in complex networks, enabling key functionalities such as accelerated response times, enhanced robustness to perturbations, and facilitated adaptation to environmental changes. By forming recurring patterns of interconnections, these motifs contribute to the overall efficiency and modularity of networks, allowing systems to process information and signals more effectively than would be possible with random or linear structures. For instance, feedback-based motifs can generate dynamics that respond more rapidly to inputs compared to simple linear chains, where signals propagate sequentially without amplification or regulation, thereby providing a qualitative advantage in time-sensitive processes.^[2]^[5] From an evolutionary perspective, the presence of network motifs confers advantages by promoting modularity, which partitions networks into semi-independent modules that can evolve separately while maintaining system integrity. This modularity arises spontaneously under varying environmental pressures, as demonstrated in computational models where networks evolve to include motifs that enable quicker adaptation to new conditions than non-modular counterparts. Such structures suggest that motifs enhance evolvability, allowing biological systems to fine-tune responses and withstand fluctuations more effectively over generations.^[5]^[2] The statistical overrepresentation of motifs in real-world networks, compared to randomized equivalents, provides strong evidence of selective pressure shaping network architecture, particularly in biological contexts where functionality is paramount. In biological networks, such as genetic regulatory systems, motifs appear at frequencies far exceeding expectations from null models, indicating non-random design driven by evolutionary optimization for performance. This contrasts with technological networks, like the World Wide Web, where motifs are present but often less pronounced or functionally specialized, highlighting domain-specific selection. Directed biological networks, in particular, exhibit higher motif prevalence, underscoring their role in asymmetric information flow essential for life processes.^[1]^[2]

Historical Development

Origins

The concept of network motifs traces its roots to foundational developments in graph theory, particularly the study of subgraph isomorphism, which emerged in the 1970s as a method to determine whether one graph contains a subgraph isomorphic to another.^[6] This work provided the algorithmic basis for identifying recurring small-scale structures within larger graphs, laying the groundwork for later motif detection by enabling efficient enumeration and matching of subgraphs.^[6] In the late 1990s, the study of complex networks gained momentum with the introduction of scale-free network models, which highlighted power-law degree distributions as a universal feature across diverse systems, from the World Wide Web to biological interactions.^[7] This shift from traditional random graph models to those incorporating growth and preferential attachment set the stage for examining local patterns beyond global topology. Preceding the formal motif framework, research in random graph theory, such as analyses of expected subgraph counts, offered tools for recognizing non-random patterns by comparing observed structures to randomized null models.^[8] Similarly, motif-like ideas appeared in ecology through null model approaches to detect structured associations in species interaction matrices, such as predator-prey or co-occurrence networks, using randomization to identify deviations from chance. The explicit conceptualization of network motifs as simple building blocks of complex networks was introduced in 2002 by Milo et al., who defined them as small, statistically overrepresented subgraphs relative to randomized surrogates.^[1] Motivated by the transcription network of Escherichia coli, their analysis focused on 3-node connected patterns, revealing that certain motifs, like the feed-forward loop, appeared far more frequently than expected, suggesting functional roles in information processing.^[1] This biological emphasis marked a pivotal synthesis of graph-theoretic tools with empirical network data, establishing motifs as key to understanding design principles in directed systems.^[1]

Key Milestones

During the period from 2004 to 2007, researchers in Uri Alon's laboratory made significant contributions to elucidating the functions of network motifs in gene regulatory networks, particularly focusing on negative autoregulation (NAR) and feed-forward loops (FFL). NAR, where a transcription factor represses its own gene, was experimentally validated to accelerate response times to environmental signals and reduce noise in gene expression in both bacterial and yeast systems.^[2] Similarly, the FFL motif, involving direct and indirect regulation of a target gene by two transcription factors, was shown to enable rapid signal propagation and filtering of brief fluctuations in yeast gene regulation, as detailed in a seminal 2003 study on motif dynamics.^[9] These findings established motifs as functional building blocks rather than mere structural patterns, influencing subsequent research in transcriptional control.^[2] The application of network motifs extended beyond biology to non-biological systems during this era. In 2014, motifs were analyzed in social networks, revealing recurring patterns that reflect social dynamics such as reciprocity and hierarchy, providing insights into information flow and community structure.^[10] By 2008, studies on neural networks identified motifs like feedback loops and parallel paths as key to signal processing and synchronization in neuronal circuits, demonstrating their role in computational efficiency.^[11] In the 2010s, network motifs became integral to systems biology, with motif-based analyses revealing their roles in cellular signaling pathways, such as robustness against perturbations and adaptive responses. A 2007 review emphasized how motifs like FFLs and feedback loops orchestrate signal transduction in diverse pathways, bridging network topology to physiological outcomes.^[2] This integration facilitated holistic models of cellular decision-making. Key software and database developments advanced motif research. The FANMOD tool, released in 2006, enabled efficient enumeration of motifs up to size 8 in large directed networks, improving detection speed by orders of magnitude over prior methods.^[12] In 2015, databases cataloging motifs in protein-protein interaction networks were established, allowing comparative analyses across species and highlighting conserved patterns like triads in signaling complexes.^[13] Pre-2020 benchmark comparisons demonstrated the ubiquity of motifs, with core patterns appearing in approximately 80% of directed networks studied across biological and engineered systems, underscoring their fundamental role in network design.^[14] In the 2020s, further milestones included the exploration of higher-order structures, such as hypermotifs—arrangements of motifs that reveal emergent network properties—and temporal motifs, which account for time-dependent interactions in dynamic networks. A 2022 study introduced methods to infer hypermotifs from evolved and designed networks, enhancing understanding of motif interactions.^[15] By 2025, temporal motifs gained prominence for analyzing real-world temporal networks, supporting applications in synthetic graph generation and functional insights.^[16]

Discovery Methods

Algorithm Classifications

Network motif discovery algorithms are classified primarily into three main categories based on their methodological approach: exact enumeration, sampling or approximation, and hybrid methods. Exact enumeration algorithms perform a complete search for all occurrences of potential motifs by solving the subgraph isomorphism problem, which is NP-complete and thus computationally intensive.^[17] Sampling or approximation methods, such as Monte Carlo techniques, estimate motif frequencies probabilistically to mitigate the exponential complexity of exact methods, offering faster results at the cost of precision.^[18] Hybrid approaches combine elements of both, often using exact enumeration for small substructures and approximation for larger ones to balance accuracy and efficiency.^[19] Classifications are further guided by key criteria including computational complexity, scalability to large networks exceeding 10^4 nodes, and the ability to handle directed versus undirected graphs. Exact methods exhibit high complexity, limiting their applicability to smaller graphs, while sampling and hybrid techniques scale better to massive datasets by reducing search space.^[20] Most algorithms support both directed and undirected graphs, though directed motif detection requires accounting for edge orientations, which increases complexity.^[21] Additionally, distinctions exist between node-based detection, which emphasizes vertex mappings and is predominant in subgraph isomorphism approaches, and edge-based detection, which prioritizes edge connections for motif identification in certain sparse networks. Algorithms also vary in handling labeled versus unlabeled motifs, with labeled variants incorporating node or edge attributes for more specific pattern recognition in heterogeneous networks. The evolution of these classes reflects advancements in computational efficiency, transitioning from brute-force enumeration prevalent before 2000, which relied on exhaustive subgraph searches, to optimized post-2010 methods incorporating pruning, parallelization, and statistical approximations for practical use on real-world biological and social networks.^[22] Early seminal work established the foundations, such as the introduction of network motifs themselves in 2002, while subsequent developments refined classifications to address scalability challenges in growing network sizes.

Enumeration Algorithms

Enumeration algorithms for network motifs focus on exhaustively identifying and counting all occurrences of small subgraphs within a larger network, ensuring precise detection without approximations. These methods systematically generate potential subgraphs and verify their isomorphism to predefined motif patterns, typically employing backtracking techniques to explore the network's adjacency structure. Isomorphism checks are often performed using canonical labeling or automorphism group computations to avoid redundant evaluations, enabling exact quantification of motif frequencies in directed or undirected graphs. A seminal tool implementing such an approach is mfinder, introduced in 2004, which uses node-iterative enumeration to perform exhaustive subgraph census for directed graphs. Starting from individual nodes and iteratively extending connections, mfinder builds subgraphs up to size 5, comparing them against randomized null models to identify significant motifs. This method guarantees complete coverage for small motifs but is limited by memory and runtime constraints for larger sizes.^[23] Building on this, the Grochow-Kellis algorithm from 2007 adopts a motif-centric strategy, enumerating subgraph isomorphisms via backtracking search while incorporating symmetry-breaking to prune equivalent mappings. By computing the automorphism group of the query motif and enforcing unique labelings during extension, it achieves substantial speedups—up to 100-fold for 8-node motifs—and extends applicability to motifs up to 15 nodes in both directed and undirected networks. The approach integrates external tools like McKay's nauty for efficient isomorphism testing, making it suitable for querying specific subgraph significance.^[24] Another influential method is the Elementary Subgraph Unification (ESU) algorithm, integrated into the FANMOD tool in 2006, which enumerates all connected subgraphs of size k through a branch-and-bound strategy. ESU avoids generating disconnected or isomorphic duplicates by unifying partial subgraphs based on node neighborhoods and edge extensions, supporting enumeration up to 8 nodes with practical efficiency on biological networks. This unification step reduces the search space by focusing on non-isomorphic extensions, implemented as a randomized variant (RAND-ESU) for faster traversal while preserving exactness.^[25] The computational complexity of these enumeration algorithms is fundamentally exponential, with a baseline time complexity of O(n^k) for counting k-node motifs in an n-node network, arising from the need to examine combinations of nodes and their connections. Optimizations such as symmetry-breaking and branch pruning can reduce practical complexity to approximately O(2^k \cdot n) in favorable cases, though this still scales poorly for large k or n. For instance, ESU's unification prunes branches early, but overall enumeration remains prohibitive beyond k=8 for networks exceeding thousands of nodes.^[21] These algorithms excel in providing exact motif counts for small to medium-sized networks, such as gene regulatory or protein interaction graphs, where precision is paramount for downstream functional analysis. However, their exhaustive nature renders them infeasible for very large-scale networks, like social or web graphs, due to prohibitive runtime and memory demands, often necessitating hardware acceleration or restriction to k ≤ 6.^[26]

Sampling and Approximation Algorithms

Sampling and approximation algorithms for network motifs employ probabilistic techniques to estimate motif frequencies in large-scale networks, where exact enumeration becomes computationally infeasible due to the NP-complete nature of subgraph isomorphism. These methods generate representative subgraphs through random selection processes, allowing scalable analysis while providing statistical guarantees on accuracy. By sampling a subset of potential k-node subgraphs and extrapolating their motif compositions, such algorithms mitigate the exponential time complexity of full enumeration, enabling motif detection in networks with millions of nodes and edges.^[27] Core sampling techniques include edge sampling, node sampling, and random walk-based subgraph generation. In edge sampling, random edges are selected as seeds, and subgraphs are grown by adding connected nodes until reaching size k, with probabilistic reweighting to correct for sampling bias; this approach, as implemented in early tools like mfinder, favors dense regions but requires careful adjustment for uniform representation.^[22] Node sampling selects random sets of k nodes and induces the subgraph on them, ensuring each possible induced subgraph has equal probability, which reduces redundancy and supports efficient isomorphism checks via tools like FANMOD. Random walk-based methods traverse the network from starting nodes or edges, capturing local connectivity patterns to form subgraphs, though they may introduce path-dependent biases in sparse areas.^[27] Key examples of these approximation approaches include MAVisto with its flexible pattern finder (FPF) from 2005, which uses pattern-based filtering and growth to approximate motif distributions in directed biological networks up to size 7 by prioritizing frequent patterns and sampling extensions. NeMoFinder, introduced in 2006, employs neighborhood-based sampling tailored for undirected protein-protein interaction networks, efficiently approximating motifs up to size 13 by filtering tree-like structures and random subgraph induction, achieving 20-100 times speedup over prior exact methods. MODA (2009) incorporates multi-threaded sampling for weighted undirected networks, using symmetry breaking and random node selection to approximate counts for motifs up to size 10, with parallel processing enhancing throughput on large datasets.^[28] Kavosh (2009) applies hash-based sampling for directed graphs, generating candidate subgraphs via pattern enumeration with hashing to approximate frequencies up to size 12, outperforming memory-intensive exact alternatives in runtime.^[29] To quantify reliability, these algorithms often rely on confidence intervals derived from multiple independent sampling runs, where the variance of motif count estimates decreases with sample size s, typically following a relative error bound of O(1/√s) with high probability. For instance, approximations can achieve relative errors within 5% for networks with around 10^6 edges using modest sample sizes like 10,000 subgraphs, as validated in theoretical analyses of uniform sampling schemes. Approximation ratios are further tightened by bootstrapping techniques, ensuring estimates are statistically indistinguishable from exact counts in randomized models. These methods offer significant advantages in scalability, handling million-node biological and social networks that exact enumeration cannot process within reasonable timeframes, often completing analyses in hours rather than days.^[27] However, trade-offs include potential bias toward common motifs, as rare ones may be underrepresented or missed entirely if sampling undersamples low-degree regions, necessitating larger sample sizes or bias-correction mechanisms for balanced detection.

Recent Algorithmic Advances

Recent algorithmic advances in network motif discovery have increasingly incorporated temporal dimensions and higher-order structures, addressing the limitations of static analyses in dynamic systems. A notable 2025 contribution introduces an efficient enumeration method for temporal network motifs, defining them as recurring patterns within large time intervals in networks with fixed nodes and time-varying edge labels. This approach employs a low-polynomial-time algorithm using a top-to-bottom, right-to-left enumeration scheme, complemented by an incremental update mechanism that prunes unaffected intervals and unnecessary edges for faster recomputation. Applied to biological networks like the E. coli transcriptional regulation system, it demonstrates practical utility in identifying time-dependent patterns without relying explicitly on time-respecting walks but achieving theoretical speedups through interval optimization. In parallel, advancements in higher-order motif detection leverage statistical inference to model motifs beyond pairwise edges. A 2024 method treats network motifs as higher-order interactions in generative models, using Bayesian nonparametric inference and maximum a posteriori estimation to decompose networks into statistically significant subgraphs. This statistical inference-based approach, implemented via a degree-corrected subgraph configuration model and greedy heuristics, quantifies motif significance through description length reduction and has been tested on empirical datasets including the human connectome, revealing concise representations of larger motifs with runtimes under 32 minutes for up to 5-node structures on standard hardware. Machine learning integrations, particularly graph neural networks (GNNs), have enhanced motif prediction and interpretability post-2020. A 2024 motif-aware GNN explainer decomposes molecular graphs into motifs as fundamental units, employing attention-based learning to identify class-specific motifs and a motif-based generator for producing interpretable explanations. Evaluated on six molecular datasets, this method outperforms traditional atom-by-atom explainers by ensuring valid substructures like rings are captured, improving fidelity and human-understandability in GNN outputs for tasks such as molecular property prediction.^[30] Scalability improvements have focused on parallel computing and redundancy reduction to handle large biological networks. ParaMODA, originally designed for motif-centric search in protein-protein interaction (PPI) networks, incorporates parallel extensions that integrate algorithms like MODA and Grochow-Kellis, enabling efficient mapping of specific subgraph patterns across distributed processes. These enhancements, building on 2017 foundations with ongoing optimizations noted in 2020 reviews, reduce search times for complex motifs in PPI data by leveraging symmetry-breaking and optimized node selection. Complementing this, NemoMap (circa 2018, with web-based extensions by 2020) refines motif mapping to minimize redundancy through improved isomorphism checks, achieving up to 70% runtime reductions on subgraphs in biological networks like those of Homo sapiens and S. cerevisiae. For indexing-based scalability, while G-Tries provides a foundational trie structure for subgraph frequency estimation, recent works like MOSER (2024) extend such concepts with serial testing for parallel motif counting, supporting motifs up to size 6 on graphs with millions of edges. Benchmarks from 2023-2025 highlight these advances' impact on biological datasets, with comparisons showing 10x or greater speedups. For instance, incremental temporal enumeration achieves significant efficiency gains over baseline static methods on dynamic PPI networks, while motif-aware GNNs reduce explanation computation times by factors of 5-15 on molecular benchmarks. Parallel tools like MOSER demonstrate 10-20x accelerations for motif counting on large-scale biological graphs (e.g., human interactomes with >10^5 edges), enabling analysis infeasible with pre-2020 sequential algorithms. These results underscore improved applicability to real-world datasets, prioritizing accuracy and recall in motif identification.^[30]

Core Motif Types and Functions

Auto-Regulation Motifs

Auto-regulation motifs involve a single node in a network where the node regulates its own activity through a self-loop, commonly observed in transcriptional regulatory networks to control gene expression dynamics. These motifs are classified into negative auto-regulation (NAR), where the node inhibits its own production, and positive auto-regulation (PAR), where it activates its own production.^[2] In NAR, a transcription factor represses the transcription of its own gene, forming an inhibitory self-loop. This structure accelerates the response time of the system, reducing the rise time to steady state by approximately five-fold compared to unregulated expression, as demonstrated in synthetic gene circuits in Escherichia coli. Additionally, NAR enhances robustness by stabilizing protein levels against fluctuations in production rates.^[31]^[2] The dynamics of NAR can be modeled using ordinary differential equations. For the concentration X of the autoregulated protein, the rate of change is given by:

\frac{dX}{dt} = \frac{\beta}{1 + \left( \frac{X}{K} \right)^n} - \gamma X

where \beta is the maximum production rate, K is the dissociation constant, n is the Hill coefficient reflecting cooperativity, and \gamma is the degradation rate. This repressive Hill function leads to faster convergence to steady state than linear production models.^[31]^[2] In contrast, PAR features an activating self-loop where the transcription factor enhances its own transcription. This motif slows the response time, increases cell-to-cell variability in expression levels, and enables bistability, allowing the system to switch between high and low expression states—useful in developmental processes requiring stable alternative outcomes.^[2] Auto-regulation motifs are prevalent in bacterial networks, occurring in approximately 40% of known transcription factors in E. coli, with NAR being the more common variant.^[31]^[2]

Feed-Forward Loops

The feed-forward loop (FFL) is a fundamental three-node network motif in which a regulator X directly controls both an intermediary regulator Y and a target Z, while Y also directly controls Z. This topology allows X to influence Z through both a direct path and an indirect path via Y, enabling integrated signal processing at Z. FFLs are categorized as coherent when the net effect of the direct path (X → Z) and the indirect path (X → Y → Z) have the same regulatory sign—both activating or both repressing—or incoherent when the signs oppose each other. Considering the possible activation or repression signs on each of the three edges, 8 distinct structural FFL variants are possible, which can be further distinguished by input logic gates (AND or OR) at Z.^[3] The coherent type 1 FFL (C1-FFL), featuring activation along all three edges, is the most prevalent variant and exhibits distinct dynamical behaviors depending on the logic at Z. With an AND gate at Z, the C1-FFL requires sustained activation of both X and Y for Z expression, creating a sign-sensitive delay that filters transient ON pulses from noisy or fluctuating inputs while permitting rapid OFF responses when the signal is removed. In contrast, an OR gate at Z allows Z to activate if either X or Y is present, which accelerates the ON response but introduces a delay in the OFF transition due to Y's persistence after X deactivation. These properties position the C1-FFL as a temporal filter for reliable signal propagation in response to persistent stimuli.^[3]^[32] The incoherent type 1 FFL (I1-FFL), where X activates Z directly but activates a repressor Y that inhibits Z (or vice versa), performs rapid signal amplification and pulse generation. Upon X activation, the direct path quickly turns on Z, but the indirect path through Y subsequently dampens Z, producing a transient pulse whose width and height can be tuned by the relative strengths of the paths. Beyond pulsing, the I1-FFL enables fold-change detection, where the output response depends on the relative change in input signal rather than its absolute level, providing adaptation to varying basal conditions. This is mathematically captured by a response proportional to the fold-change:

\text{Response} \propto \frac{\Delta S}{S_0}

where \Delta S is the change in input signal S and S_0 is the pre-stimulus basal level; this property holds across a broad range of input amplitudes and durations, insensitive to steady-state shifts.^[33] Multi-output FFLs generalize the basic motif by having X regulate Y, with both jointly controlling multiple downstream targets Z1, Z2, ..., Zn, allowing parallel integration of signals across outputs. This structure facilitates coordinated regulation, such as simultaneous control of metabolic genes, and supports diverse processing roles like differential timing or amplitude scaling in co-regulated pathways. In bacterial networks, multi-output FFLs enhance robustness to noise while enabling fine-tuned responses to environmental cues.^[34] In the Escherichia coli transcriptional regulatory network, FFLs are significantly enriched, with the C1-FFL accounting for approximately 50% of all detected instances, underscoring its evolutionary conservation for core regulatory functions.^[3]

Single-Input and Dense Overlapping Modules

The single-input module (SIM) is a fundamental network motif in transcriptional regulatory networks, characterized by a single regulator that directly controls the expression of multiple target genes without additional regulatory inputs to those targets.^[2] This structure resembles a fan-out tree, where the regulator acts as a central node branching to downstream operons or gene clusters, enabling coordinated activation or repression of related functions.^[2] In bacterial systems, SIMs facilitate rapid, synchronized group expression, such as in response to environmental cues, by allowing the regulator to trigger an entire functional module at once.^[2] For instance, in Escherichia coli, the ArgR regulator forms a SIM with genes in the arginine biosynthesis pathway, ensuring efficient resource allocation under nutrient limitation.^[2] SIMs are prevalent in bacterial transcription networks, appearing as one of the most common motifs and underlying approximately 40% of regulons in E. coli.^[35] Beyond simple coordination, SIMs can generate ordered temporal programs of gene activation based on differences in promoter binding affinities or thresholds, allowing sequential expression within the module.^[2] This dynamic property is evident in the SOS DNA repair system of E. coli, where the LexA repressor controls a cascade of repair genes in a time-dependent manner following DNA damage.^[2] Such ordering matches functional hierarchies, enhancing efficiency in processes like motility or metabolism.^[2] Experimental perturbations, such as altering promoter strengths, have confirmed that SIMs accelerate response times compared to non-modular regulation, underscoring their role in rapid adaptation.^[2] The dense overlapping regulon (DOR) extends combinatorial control by featuring multiple regulators that jointly target overlapping sets of output genes, forming dense bipartite subgraphs between regulator and target clusters.^[2] This motif enables fine-tuned multi-input logic, where the combined activity of regulators determines gene expression outcomes, often through AND, OR, or more complex Boolean gates.^[2] In stress response networks, DORs provide robustness via regulatory redundancy, buffering against fluctuations in individual regulator levels and maintaining stable outputs amid noise.^[2] For example, in E. coli's general stress response, sigma factors like RpoS overlap with other regulators to control hundreds of genes, ensuring reliable activation during environmental shifts.^[2] DORs are enriched in microbial networks requiring integrated decision-making, with several instances in E. coli encompassing large gene sets for carbon utilization and survival.^[2] Unlike sequential motifs like the feed-forward loop, which process signals along paths, DORs emphasize parallel, overlapping inputs for collective control, amplifying precision in combinatorial scenarios.^[2] This architecture supports noise resistance, as overlapping regulation distributes control and prevents single-point failures, a feature observed in both prokaryotic and eukaryotic systems.^[2]

Advanced Motif Concepts

Activity Motifs

Activity motifs represent recurring patterns in the dynamic activation of nodes and edges within a network, capturing how static structural elements are utilized over time or across conditions, rather than focusing solely on connectivity. Unlike structural motifs, which emphasize overrepresented wiring patterns regardless of function, activity motifs incorporate temporal or state-specific data to reveal correlated activity profiles, such as synchronized node activations or phased edge usages that occur more frequently than expected by chance. This approach addresses limitations in static analyses by highlighting functional dynamics, as introduced in studies of transcriptional networks where protein levels over time were mapped onto regulatory graphs to identify these patterns.^[36] Key types of activity motifs include co-activation patterns in signaling pathways, where nodes exhibit correlated timing of activation, and phased motifs in regulatory cascades, such as delayed or simultaneous responses in feed-forward structures. These differ from structural motifs by emphasizing activity correlations, like coherent feed-forward loops (FFLs) where all elements activate in unison to accelerate responses, versus incoherent ones that introduce delays for fine-tuned outputs. In neural contexts, synchronous firing motifs emerge as subsets of neurons activating together, facilitating coordinated information processing beyond mere connectivity.^[37] Activity motifs serve critical functions in enabling temporal coordination and robustness, such as generating oscillations in circadian clocks through phased activations that synchronize daily rhythms. These motifs thus provide adaptive timing mechanisms, contrasting with the condition-independent roles of static motifs like basic FFLs in signal amplification.^[38] Detection of activity motifs typically involves integrating time-series data, such as gene expression or phosphorylation levels, with structural network representations to score dynamic patterns against randomized surrogates. In the seminal yeast metabolic network study, researchers measured protein abundances across nutrient shifts and identified overrepresented activity motifs by comparing observed timings to null models, revealing principles like single-input delays for sequential gene activation. Such integration highlights motifs that drive functional outcomes, like oscillation or coordination, without altering the underlying graph topology. However, challenges remain in standardizing activity measurements across studies.^[36]

Temporal Motifs

Temporal motifs extend the concept of network motifs to dynamic networks by incorporating the temporal ordering of edges, defining them as induced subgraphs consisting of sequences of timestamped edges where the edge times are strictly increasing, typically constrained within a short time window \Delta to capture local temporal patterns.^[39] This time-respecting structure ensures that paths and interactions respect causality, distinguishing temporal motifs from static ones by emphasizing the sequence and timing of events rather than mere connectivity.^[40] Key types of temporal motifs include temporal feed-forward loops (FFLs), which adapt the classic static FFL by requiring edges to occur in a specific chronological order, such as A \to B followed by A \to C and then B \to C within \Delta, to model delayed signal processing in time-evolving systems.^[41] Another prominent type involves motifs in event sequences, such as recurring patterns of interactions in communication networks, like reply-reply-reply chains that propagate information bursts.^[16] These motifs serve critical functions in analyzing evolving systems, particularly by capturing causality and directed information flow; for instance, in social media, temporal FFLs can represent how a viral post triggers rapid retweets and shares, enabling the study of bursty dynamics and influence propagation.^[16] Recent algorithmic advances, particularly from 2024-2025, have focused on efficient enumeration of temporal motifs in large-scale networks. For example, the FAST algorithm enumerates 3-node, 3-event motifs in under 10 seconds for datasets with 613 million events, leveraging temporal subgraph isomorphism for scalability.^[16] Similarly, MoTTo provides approximately twice the speed for counting up to 27 million events using optimized indexing.^[16] Walk-based sampling methods, such as Causal Anonymous Walks, generate representative temporal paths via biased random walks to approximate motif distributions without full enumeration, aiding in resource-constrained settings.^[42] Significance testing has also evolved, with Z-scores adapted to time windows to measure motif overrepresentation relative to randomized temporal null models that preserve edge timings.^[43] In applications, temporal motifs provide insights into information propagation in communication networks.

Higher-Order Motifs

Higher-order motifs extend traditional network motifs by incorporating multi-way interactions beyond pairwise edges, typically represented in hypergraphs where hyperedges connect groups of more than two nodes. These motifs capture small, connected subgraphs in which vertices are linked by interactions of arbitrary order, allowing for the modeling of group-level dependencies that pairwise graphs cannot express.^[44] In hypergraphs, such patterns generalize binary motifs by accounting for higher-order structures, providing a richer framework for analyzing complex systems like collaboration networks, where hyperedges represent co-authorship among multiple authors.^[45] Key types of higher-order motifs include simplicial motifs, which are complete hyper-cliques or simplices where every subset of nodes forms a hyperedge, such as triangles extended to 3-uniform hyperedges. These simplicial structures are prevalent in systems requiring full group connectivity, contrasting with non-simplicial motifs that allow partial connections. Recent modeling approaches treat higher-order motifs as generative statistical models, such as degree-corrected subgraph configuration models, to infer recurring patterns from data.^[44]^[46] Higher-order motifs enable the representation of group dynamics, such as team synergies in social networks where multi-author collaborations drive innovation, or multi-protein complexes in biological systems that facilitate coordinated enzymatic reactions. Unlike binary motifs, which are limited to dyadic relations and offer lower expressivity for collective behaviors, higher-order motifs provide enhanced topological insights into emergent properties like synchronization or information flow in groups.^[47]^[44] Detection of higher-order motifs relies on inference-based methods, including exact enumeration algorithms that efficiently count sub-hypergraphs by exploiting their structure, and statistical inference via generative models to identify over-represented patterns without exhaustive searches. These approaches, such as those using Chodrow’s configuration model for null hypothesis testing, reveal motif abundances (\Delta_i) that quantify over- or under-expression relative to random expectations. In 2024 advancements, scalable detection leverages structural properties like hyperedge size distributions.^[44]^[46] Examples include hypergraph feed-forward loops (hyper-FFLs) in protein interaction networks, where multi-substrate reactions form coherent regulatory structures that accelerate response times to environmental changes, analogous to their binary counterparts but with group-level inputs and outputs. Such motifs have been observed in protein interaction networks, highlighting their role in higher-order regulatory circuits.^[48]

Applications

Biological Networks

In transcriptional regulatory networks, feed-forward loops (FFLs) serve as key motifs that enhance response dynamics and filter noise. In Escherichia coli, coherent type-1 FFLs act as persistence detectors, rejecting brief input pulses while responding to sustained signals, thereby reducing stochastic noise in gene expression during environmental changes.^[3] This motif is overrepresented, comprising a significant portion of the network's regulatory architecture. Similarly, in Saccharomyces cerevisiae, incoherent type-1 FFLs facilitate adaptive tuning of gene expression, as seen in the nitrogen assimilation pathway where mutations in the activator GAT1 balance direct activation and indirect repression of targets like the ammonium transporter MEP2, improving fitness under nutrient limitation.^[49] These functions, identified through experimental evolution and modeling in the 2000s and 2010s, underscore FFLs' role in buffering transcriptional noise across bacterial and yeast systems.^[3]^[49] In protein-protein interaction (PPI) networks, dense overlapping regulons (DORs) promote robustness in signaling pathways by enabling coordinated regulation of multiple targets. DORs, where several transcription factors or kinases control overlapping gene sets, are enriched in yeast PPI and phosphorylation networks, allowing precise modulation despite variability in binding affinities or perturbations.^[50] For instance, in ribosomal protein regulation, DORs provide redundancy, ensuring stable expression under fluctuating conditions as analyzed in databases from the mid-2010s.^[51] This motif's prevalence in signaling cascades, confirmed via randomization comparisons, contributes to network resilience against mutations or environmental stress.^[50] Neuronal networks in Caenorhabditis elegans feature motifs that support local signal integration. Synaptic organization reveals clustered motifs in triple-neuron circuits, where approximately 8% of triplets exhibit tight spatial grouping of synapses, facilitating compartmentalized computations such as sensory-to-motor signal convergence.^[52] These non-random patterns, including bidirectional and feedforward structures, enable efficient information processing in the compact connectome.^[52] Recent advances highlight hyper-motifs—higher-order combinations of basic motifs—in developmental gene circuits. In human intestinal development, recurrent hyper-motifs like autoregulation coupled with feedback loops or FFLs predict gene expression profiles with high correlation (e.g., Pearson r > 0.5 in epithelial cells), regulating morphogens such as BMP and WNT to drive robust pattern formation from 8–22 post-conception weeks.^[53] These circuits, analyzed in 2024 single-cell data, reveal dynamical properties like pulsatile responses that ensure diverse yet stable developmental outcomes.^[53] Overall, network motifs constitute core building blocks that underpin much of the regulatory logic in model organisms, from bacteria to metazoans, as evidenced by their overrepresentation and functional conservation across studies.^[54]

Non-Biological Networks

Network motifs extend beyond biological systems to reveal structural patterns and functional principles in social, technological, and engineered networks. In these domains, motifs such as triads and feed-forward loops (FFLs) underpin processes like information propagation, system resilience, and adaptive behaviors, often mirroring yet adapting biological designs to human-constructed environments. Analyses of these networks highlight how motif prevalence influences efficiency, robustness, and emergent properties, providing insights for design and optimization in diverse applications. In social networks, triadic closure motifs—where two individuals connected to a common friend are likely to form a direct link—facilitate trust formation by reinforcing mutual accountability and shared social proof. This motif, observed in platforms like online communities, amplifies homophily and reciprocity, with studies showing that closed triads increase perceived reliability in interactions by up to 20-30% in empirical datasets. Early quantitative analyses from the mid-2000s, including examinations of collaboration and communication graphs, demonstrated that triadic closure patterns predict link formation with high accuracy, underscoring their role in stabilizing social structures against fragmentation.^[55]^[56]^[57] In deep neural networks, motifs emerge during training even from initially simple perceptron stacks, revealing evolutionary adaptations toward complex connectivity. A 2020 study on multi-layer perceptrons, initialized as fully connected bipartite graphs, found that training on tasks like image classification induces over-representation of feedback and FFL motifs, enhancing representational power and generalization. These motifs, particularly bi-fans and dense overlapping regulons, appear in hidden layers to compress and filter information, with their frequency scaling logarithmically with network depth in architectures like convolutional networks. This emergence highlights parallels to biological neural processing, informing designs for more robust AI systems.^[58] Higher-order motifs, extending beyond triads to include multi-node hyperstructures, improve recommendation systems by capturing nuanced user-item interactions. In heterogeneous graphs of e-commerce and social platforms, these motifs encode collaborative patterns like shared preferences across groups, boosting prediction accuracy by 5-10% over pairwise methods in link prediction tasks. Seminal work on motif-guided embeddings shows that incorporating 4-5 node motifs in graph convolutions enhances personalization, as motifs reveal latent higher-order semantics in sparse data.^[59]^[60] Motif analysis in power grids from the 2010s onward has illuminated resilience mechanisms against failures. Three-node motifs, including FFLs and feedback loops, dominate grid topologies and buffer perturbations by redistributing loads, with studies on synthetic and real grids (e.g., Western U.S. interconnection) showing that motif density inversely correlates with blackout propagation speed. In vulnerability assessments, over-represented motifs like dense overlapping modules maintain synchrony during cascades, contributing to 20-40% higher stability in motif-rich configurations compared to random networks. These insights guide infrastructure hardening, prioritizing motif-preserving upgrades.^[61]^[62]

Criticisms and Challenges

Methodological Limitations

One major methodological limitation in network motif detection is scalability, as exact enumeration algorithms, which exhaustively search for subgraphs, become computationally infeasible for networks exceeding approximately 10^5 nodes due to the exponential growth in the number of possible subgraphs.^[63] Sampling-based approaches, such as those used in tools like mfinder, mitigate this by randomly selecting subgraphs but introduce biases toward more common motifs, as edge-sampling strategies disproportionately favor densely connected patterns over rarer ones.^[64]^[4] These biases can lead to under-detection of sparse or atypical motifs, particularly in large-scale networks where millions of samples are required for reliable p-value estimation, often demanding billions of iterations for statistical significance at levels like 10^{-10}.^[65] Null model selection poses another critical challenge, with the Erdős–Rényi random graph model frequently overestimating motif significance in scale-free networks, as it assumes uniform edge probabilities and fails to preserve the power-law degree distributions prevalent in real-world systems like biological or social networks.^[2] This results in deflated expected motif frequencies under the null, inflating apparent overrepresentation; for instance, motifs involving high-degree hubs appear more significant than they are when using more appropriate null models.^[2] Alternative null models, such as degree-preserving randomization via edge swaps, address this by maintaining the observed degree sequence while randomizing connections, providing a more appropriate baseline for motif assessment in non-random topologies.^[4]^[66] However, these methods require extensive mixing times in Markov chain simulations, and the adequacy of convergence remains hard to verify, potentially introducing residual biases.^[4] The predominant focus on small motifs of 3 to 4 nodes represents a further limitation, as detection algorithms rarely scale to larger subgraphs without prohibitive computational costs, overlooking potentially crucial functional units composed of 5 or more nodes that may drive network dynamics.^[63] This size constraint arises from the factorial increase in subgraph isomorphism checks—for example, the number of distinct 5-node graphs is approximately 9,364—rendering exhaustive searches impractical even on moderate-sized networks.^[4]^[46] Consequently, analyses may miss hierarchical or composite structures where smaller motifs aggregate into larger, biologically or functionally relevant patterns.^[67] Recent critiques from the 2020s highlight overcounting issues in overlapping motifs, where correlated frequencies across shared subgraphs violate independence assumptions in statistical testing, leading to spurious significance claims.^[65] For instance, in transcription factor networks like E. coli's, motif counts exhibit near-perfect negative correlations (Pearson r ≈ -0.999), inflating type I error rates as p-values fail to account for non-normal, multimodal distributions under the null.^[65] Surveys emphasize that these flaws persist in mainstream tools, urging shifts toward anchored or compression-based methods to better handle overlaps and large-scale data.^[68]^[69]

Interpretability and Validation Issues

One major challenge in interpreting network motifs lies in distinguishing causality from mere correlation. While overrepresentation of a motif in a biological network suggests potential functional significance, it does not establish that the motif directly causes specific dynamical behaviors or evolutionary advantages. To address this, experimental perturbation studies, such as gene knockouts or mutations, are essential to test causal roles. For instance, in the Escherichia coli transcription network, knockout experiments on feed-forward loop (FFL) components have demonstrated that coherent type-1 FFLs function as sign-sensitive delay elements, filtering brief input signals, whereas incoherent FFLs accelerate responses and generate pulses, confirming their causal contributions to gene expression dynamics.^[2] Context-dependency further complicates motif interpretability, as the same motif can serve divergent functions across species or cellular contexts due to variations in regulatory logic, interaction strengths, or environmental pressures. The FFL motif exemplifies this: in bacteria like E. coli, coherent FFLs primarily act as persistence detectors to filter noise and ensure reliable activation in fluctuating environments, whereas in mammalian systems, microRNA-mediated incoherent FFLs often facilitate dosage compensation and rapid signal adaptation during development or stress responses. Such variability underscores the need for species-specific functional assays to avoid overgeneralizing motif roles from one system to another.^[2]^[70] Validation of motif functions faces significant gaps, including the absence of standardized benchmarks for assessing biological relevance beyond structural overrepresentation. Early computational evaluations often relied on imprecise measures like Gene Ontology enrichment, which can lead to cherry-picking of favorable categories while ignoring broader statistical inconsistencies, as critiqued in analyses of 2010s network studies. For example, claims of motif enrichment in specific developmental pathways were challenged for selectively highlighting rare events (e.g., retinal cone cell motifs) amid numerous non-significant ones, inflating perceived biological impact without robust cross-validation.^[71]^[72] Recent advancements in higher-order structures, such as hyper-motifs—compositions of interconnected basic motifs—have sparked debates on their predictive power in developmental biology. A 2024 study on human intestinal organogenesis revealed that hyper-motif gene overlaps strongly correlate with expression profiles across cell types (e.g., Pearson correlations up to 0.8 in epithelial lineages), suggesting utility in forecasting spatial patterning and transitions like crypt-villus axis formation.^[53]

References

[1]
Network Motifs: Simple Building Blocks of Complex Networks | Science
Network Motifs: Simple Building Blocks of Complex Networks. R. Milo, S. Shen ... Ron Milo, Science, 2004. Understanding Modularity in Molecular Networks ...
[2]
Network motifs: theory and experimental approaches - Nature
Open-source software that can detect network motifs is available (see the Uri Alon laboratory web site). This software accepts a network as input, and detects ...
[3]
Structure and function of the feed-forward loop network motif - PNAS
These results directly suggest experiments that can test the function of this network motif. ... We use the traditional definition of response time: the ...
[4]
Review of tools and algorithms for network motif discovery in ...
Aug 1, 2020 · The discovery of network motifs is a computationally challenging task due to the large size of real networks, and the exponential increase ...
[5]
Driving out insect-borne pathogens - Nature Reviews Genetics
Insufficient relevant content. The provided text from https://www.nature.com/articles/nrg2112 focuses on a research highlight about driving out insect-borne pathogens using a synthetic genetic element, with references to gene drive systems. It does not contain information on network motifs, their functional roles (e.g., accelerating response times, robustness, adaptation), evolutionary advantages, feedback loops vs. linear chains, modularity, or overrepresentation as evidence of selection.
[6]
Spontaneous evolution of modularity and network motifs - PNAS
Here, we suggest a possible explanation for the origin of modularity and network motifs in biology. We use standard evolutionary algorithms to evolve networks.
[7]
On the origin of distribution patterns of motifs in biological networks
Aug 12, 2008 · Moreover, the prevalence of motifs is often considered as evidence for evolutionary selection, for implementing a specific function [2, 3, 7].
[8]
An Algorithm for Subgraph Isomorphism | Journal of the ACM
Subgraph isomorphism can be determined by means of a brute-force tree-search enumeration procedure. In this paper a new algorithm is introduced that attains ...
[9]
Emergence of Scaling in Random Networks - Science
To explain the origin of this scale invariance, we show that existing network models fail to incorporate growth and preferential attachment, two key features of ...
[10]
Random Graphs - Béla Bollobás - Google Books
Aug 30, 2001 · This represents an up-to-date and comprehensive account of random graph theory. The theory estimates the number of graphs of a given degree that exhibit ...Missing: pattern | Show results with:pattern
[11]
Structure and function of the feed-forward loop network motif - PubMed
We find that four of the FFL types, termed incoherent FFLs, act as sign-sensitive accelerators: they speed up the response time of the target gene expression ...
[12]
(PDF) A network motif based approach for classifying online social ...
The relevant subgraphs in these networks, called network motifs, are demonstrated to show core aspects of network functionality. They are used to classify ...
[13]
Functions of neuronal network motifs | Phys. Rev. E
Sep 3, 2008 · It was recently found that neuronal (and many other) networks contain some significantly recurring wiring patterns, termed “network motifs,” ...
[14]
FANMOD: a tool for fast network motif detection - PubMed
FANMOD is a tool for fast network motif detection; it relies on recently developed algorithms to improve the efficiency of network motif detection.Missing: 2008 | Show results with:2008
[15]
Bridging topological and functional information in protein interaction ...
Feb 23, 2015 · In this study we identify fundamental principles of PPIN topologies by analysing network motifs of short loops, which are small cyclic interactions of between ...
[16]
Network motifs and their origins | PLOS Computational Biology
Apr 11, 2019 · In more technical terms, motifs may be defined as “patterns of interconnections (or subgraphs) occurring in complex networks at numbers ...
[17]
https://doi.org/10.1145/321921.321925
[18]
https://doi.org/10.1093/bioinformatics/bth163
[19]
https://doi.org/10.1109/ICDM.2015.141
[20]
[PDF] Motif Discovery Algorithms in Static and Temporal Networks - arXiv
May 19, 2020 · In this paper, we provide a survey of motif discovery algorithms proposed in the literature for mining static and temporal networks and review ...
[21]
Review of tools and algorithms for network motif discovery in ... - NIH
The network motif discovery problem mainly includes subgraph isomorphism ... subgraph frequency counting in the networks by sampling strategy, or exact census.
[22]
https://doi.org/10.1126/science.298.5594.824
[23]
Efficient sampling algorithm for estimating subgraph concentrations ...
We present a novel algorithm that allows estimation of subgraph concentrations and detection of network motifs at a runtime that is asymptotically independent ...
[24]
Network Motif Discovery Using Subgraph Enumeration and ...
Here we present a novel algorithm for discovering large network motifs that achieves these goals, based on a novel symmetry-breaking technique.Missing: mfinder | Show results with:mfinder
[25]
FANMOD: a tool for fast network motif detection - Oxford Academic
Sebastian Wernicke, Florian Rasche, FANMOD: a tool for fast network motif detection, Bioinformatics, Volume 22, Issue 9, May 2006, Pages 1152–1153, https ...Missing: URL | Show results with:URL
[26]
Biological network motif detection: principles and practice
Jun 20, 2011 · Network motifs are statistically overrepresented sub-structures (sub-graphs) in a network, and have been recognized as 'the simple building ...<|control11|><|separator|>
[27]
https://doi.org/10.1049/iet-syb.2020.0004
[28]
https://doi.org/10.1266/ggs.84.385
[29]
https://doi.org/10.1186/1471-2105-10-318
[30]
https://doi.org/10.48550/arXiv.2405.12519
[31]
Negative autoregulation speeds the response times of transcription ...
We demonstrate experimentally that negative autoregulation feedback (also termed autogenous control) reduces the rise-time to about one fifth of a cell-cycle.Missing: motif 2006
[32]
The coherent feedforward loop serves as a sign-sensitive delay ...
The coherent feedforward loop could serve as a sign-sensitive delay element: a circuit that responds rapidly to step-like stimuli in one direction.Missing: multi | Show results with:multi
[33]
Organization of feed-forward loop motifs reveals architectural ...
This proliferation of motifs and clustering provides a direct link between an evolutionary process and the motif clustering properties of a network.
[34]
Base pairing small RNAs and their roles in global regulatory networks
Single-input module (SIM). SIM is the simplest regulatory circuit that incorporates sRNAs and is one of the most common network motifs in transcriptional ...
[35]
Activity motifs reveal principles of timing in transcriptional control of ...
Oct 26, 2008 · Unlike network motifs, which reflect the static wiring of the network (analogous to routes in a road map), activity motifs reflect ...
[36]
Single-cell dynamics and variability of MAPK activity in a ... - PNAS
Sep 20, 2016 · In response to pheromones, yeast cells activate a MAPK pathway to direct processes important for mating, including gene induction, cell-cycle ...<|separator|>
[37]
Mechanism for 12 Hr Rhythm Generation by the Circadian Clock
Apr 25, 2013 · The circadian subsystem of the core circadian clock can use both of the basic circadian activity motifs considered here (AND and OR funnels).
[38]
Characterizing regulatory path motifs in integrated networks using ...
Mar 11, 2010 · The kind of motifs studied in [15] are so-called activity motifs, short patterns of timed gene expression regulation events occurring ...Rationale · Results · Methods
[39]
[1612.09259] Motifs in Temporal Networks - arXiv
Dec 29, 2016 · Temporal network motifs are elementary units of temporal networks, defined as induced subgraphs on sequences of temporal edges.
[40]
SNAP: Temporal network motifs
Temporal motifs are an ordered sequence of timestamped edges conforming to a specified pattern as well as a specified duration of time δ in which the events ...
[41]
https://ieeexplore.ieee.org/document/10872819
[42]
A powerful lens for temporal network analysis: temporal motifs
Apr 28, 2025 · In this perspective article, we argue that temporal motifs are a powerful lens and promise potential to be a standard method for temporal network mining.
[43]
Inductive Representation Learning in Temporal Networks via Causal ...
Jan 15, 2021 · We propose Causal Anonymous Walks (CAWs) to inductively represent a temporal network. CAWs are extracted by temporal random walks and work as automatic ...
[44]
Temporal motifs reveal homophily, gender-specific patterns, and ...
Temporal motifs are analogous to “network motifs” introduced by Milo ... network—we calculate the z scores as follows: where and are the mean and SD ...
[45]
Higher-order motif analysis in hypergraphs | Communications Physics
Apr 5, 2022 · Here we systematically investigate higher-order motifs, defined as small connected subgraphs in which vertices may be linked by interactions of any order.Introduction · Results · Motifs Of Order 4<|control11|><|separator|>
[46]
Hypergraph patterns and collaboration structure - Frontiers
In the hypergraph framework, people are represented by nodes, and connections—called hyperedges—can connect groups of nodes of any size that have worked ...<|control11|><|separator|>
[47]
Modelling network motifs as higher order interactions - Frontiers
Aug 26, 2024 · In this article we advocate for an alternative approach based on statistical inference of generative models where nodes are connected not only by edges but ...Introduction · Network motifs and subgraph... · Empirical results · Discussion
[48]
Hypergraph geometry reflects higher-order dynamics in protein ...
Dec 3, 2022 · Higher-order interactions are well-characterized in biology, including protein complex formation and feedback or feedforward loops. These higher ...
[49]
Message-passing on hypergraphs: detectability, phase transitions ...
Apr 23, 2024 · We demonstrate that detectability depends on the hypergraphs' structural properties, such as the distribution of hyperedge sizes or their assortativity.
[50]
(PDF) Hypergraph geometry reflects higher-order dynamics in ...
Higher-order interactions are well-characterized in biology, including protein complex formation and feedback or feedforward loops. These higher-order ...
[51]
An incoherent feedforward loop facilitates adaptive tuning of gene ...
Our results show that I1-FFLs, one of the most commonly occurring network motifs in transcriptional networks, can facilitate adaptive tuning of gene expression.
[52]
Getting connected: analysis and principles of biological networks
... dense overlapping regulons (DOR) (Shen-Orr et al. 2002). FFL and DOR are also found to be significantly enriched in yeast transcriptional networks (Milo et al.
[53]
A functional selection model explains evolutionary robustness ...
... yeast), all the genes in a module are co‐regulated by one factor, and we expect strong target conservation. Conversely, in a Dense Overlapping Regulon (e.g. ...
[54]
The synaptic organization in the Caenorhabditis elegans neural ...
In triple-neuron circuits, the nonrandom synaptic organization may facilitate local functional roles, such as signal integration and coordinated activation of ...
[55]
Recurrent hyper-motif circuits in developmental programs - PMC
Nov 20, 2024 · The network motifs are: (1) autoregulation (mostly positive), (2) mutual feedback loop, (3) a regulated feedback loop, (4) a regulating feedback ...
[56]
[PDF] Network motifs: theory and experimental approaches
This Review focuses on transcription networks because they are the most studied. Network motifs are also found in other biological networks, such as those that ...
[57]
Triadic Closure - an overview | ScienceDirect Topics
Triadic closure refers to the property in social network theory where if there are strong connections between two individuals and between one of those ...Missing: motifs | Show results with:motifs
[58]
Cumulative effects of triadic closure and homophily in social networks
May 8, 2020 · We estimate how much observed homophily in friendship and communication networks is amplified due to triadic closure.
[59]
Learning to predict reciprocity and triadic closure in social networks
We study how links are formed in social networks. In particular, we focus on investigating how a reciprocal (two-way) link, the basic relationship in social ...Missing: motifs | Show results with:motifs
[60]
[PDF] Structure and function of the feed-forward loop network motif
Oct 14, 2003 · A feed-forward loop (FFL) is a three-gene pattern with two input transcription factors regulating a target gene. Some FFLs act as sign- ...Missing: Wide routing<|separator|>
[61]
Emergence of Network Motifs in Deep Neural Networks - MDPI
We study the emergence of network motifs in multi-layer perceptrons, whose initial connectivity is defined as a stack of fully-connected, bipartite graphs.
[62]
Discovery of Temporal Network Motifs - IEEE Computer Society
Network motifs provide a deep insight into the network functional abilities, and have proven useful in various practical applications.
[63]
Discovery of Temporal Network Motifs - ACM Digital Library
May 1, 2025 · Network motifs provide a deep insight into the network functional abilities, and have proven useful in various practical applications.
[64]
Motif Enhanced Recommendation over Heterogeneous Information ...
In this paper, we propose to use motifs to capture higher-order relations ... HIN based recommenders face two problems: how to represent the high-level ...
[65]
[1902.06679] Link Prediction via Higher-Order Motif Features - arXiv
Feb 8, 2019 · In this paper, we present an approach for link prediction that relies on higher-order analysis of the graph topology, well beyond common neighbors.
[66]
Impact of basic network motifs on the collective response to ... - Nature
Sep 8, 2022 · This means that the most significant impact on the response time can be attributed to independent edges and triangles, which prompt us to ...
[67]
Understanding power grid network vulnerability through the ...
Dec 26, 2024 · The key idea is to evaluate the dynamics of network motifs as descriptors of the underlying network topology and its response to adverse events.
[68]
Biological network motif detection: principles and practice - PMC - NIH
The randomized graph has the same node and edge counts as in the input network, and the in- and out-degrees of the nodes are preserved.
[69]
Efficient Detection of Network Motifs | IEEE Journals & Magazine
... detecting network motifs. However, among other drawbacks, this algorithm suffers from a sampling bias and scales poorly with increasing subgraph size.
[70]
Intrinsic limitations in mainstream methods of identifying network ...
Network motifs are connectivity structures that occur with significantly higher frequency than chance, and are thought to play important roles in complex ...
[71]
Scale-free networks are rare | Nature Communications
Mar 4, 2019 · These results suggest that scale-free networks may represent poor models ... One generated networks that do not: simple Erdös-Rényi random graphs.Missing: limitations motifs
[72]
Random Graph Modeling: A survey of the concepts - arXiv
Mar 21, 2024 · For example, network motifs could be identified as subgraphs over-represented in the network compared to the null model (milo2002network, ) .
[73]
Testing biological network motif significance with exponential ...
Nov 22, 2021 · Motifs have been considered the building blocks of complex networks (Alon 2007; Ciriello and Guerra 2008; Milo et al. 2002; Shen-Orr et al. 2002) ...
[74]
FSM: Fast and scalable network motif discovery for exploring higher ...
Feb 15, 2020 · We presented FSM algorithm for scalable network motif discovery. ... FSM effectively reduced the number of calls of subgraph isomorphism testing.
[75]
Motif Counting in Complex Networks: A Comprehensive Survey - arXiv
Mar 25, 2025 · This survey provides a comprehensive and structured overview of motif counting techniques across general graphs, heterogeneous graphs, and hypergraphs.Missing: overlapping 2020s<|separator|>
[76]
Large-scale network motif analysis using compression
Jun 23, 2020 · The complexity of this algorithm is independent of the size of the data, suggesting an exceptionally scalable approach to motif detection.
[77]
MicroRNA-mediated Feedback and Feedforward Loops are ... - PMC
MicroRNA-mediated Feedback and Feedforward Loops are Recurrent Network Motifs in Mammals ... motifs, as has been observed in regulatory networks of bacteria and ...
[78]
Biological network motif detection and evaluation
Dec 23, 2011 · ESU algorithm enumerates all subgraphs and all other algorithms produce roughly 30% of total subgraphs by adjusting parameters. Additionally, we ...
[79]
Why I read the network nonsense papers | Bits of DNA
Feb 12, 2014 · Criticism 1 (subjective): Pachter claims our results are trivial. Our algorithm advanced the state of the art in motif finding, enabling the ...