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Octanol-water partition coefficient

The octanol-water partition coefficient, denoted as Kow or Pow, is a physicochemical that quantifies the of a substance between two immiscible phases—n-octanol and —at and a specified , typically 25°C. It is defined as the ratio of the concentration of the substance in the n-octanol phase (Co) to its concentration in the phase (Cw), expressed mathematically as Kow = Co / Cw, and is a dimensionless value usually reported on a as log Kow to span its wide range from hydrophilic (log Kow < 0) to highly lipophilic compounds (log Kow > 6). n-Octanol serves as a surrogate for membranes and due to its amphiphilic nature, making Kow a reliable indicator of a compound's hydrophobicity or . This coefficient plays a pivotal role in multiple scientific and regulatory domains, particularly in predicting the environmental fate and biological interactions of organic chemicals. In environmental science, log Kow is essential for modeling partitioning behaviors, such as sorption to soils and sediments, volatilization, and bioconcentration in aquatic organisms, where values exceeding 4 often signal potential bioaccumulation risks under frameworks like REACH. In pharmacology and drug design, it informs absorption, distribution, metabolism, and excretion (ADME) profiles by correlating with membrane permeability, solubility, and bioavailability, with optimal oral drug candidates typically exhibiting log Kow values between 1 and 3. Quantitative structure-activity relationship (QSAR) models further leverage Kow to estimate toxicity and ecotoxicological endpoints without extensive testing. Measurement of Kow follows standardized protocols to ensure accuracy, especially for compounds with extreme values. The OECD Test No. 123 slow-stirring method is preferred for highly hydrophobic substances (log Kow up to 8.2), involving equilibration in a stirred reactor to minimize emulsion formation, followed by concentration analysis via techniques like (HPLC) or . For volatile or low-solubility compounds, chromatographic methods—such as reversed-phase HPLC or gas-liquid chromatography—offer efficient alternatives by correlating retention times to partition behavior at infinite dilution. These approaches, validated against direct shake-flask equilibration, support reliable data for regulatory submissions and predictive modeling.

Fundamentals

Definition

The octanol-water partition coefficient, commonly denoted as P or K_{ow}, is defined as the ratio of the equilibrium concentration of a neutral organic compound in the 1-octanol phase to its concentration in the water phase, expressed as
P = \frac{[\text{solute}]_{\text{octanol}}}{[\text{solute}]_{\text{water}}}
This parameter is measured under standard conditions of 25°C and pH 7 to ensure applicability to non-ionizable, neutral species.
Due to the wide range of values typically encountered, the coefficient is often reported on a as \log P or \log K_{ow}, providing a measure of the compound's . The selection of as the organic phase stems from its amphiphilic properties—a polar hydroxyl group attached to a hydrophobic alkyl chain—which effectively mimic the structure of bilayers in biological membranes.

Physical and Chemical Significance

The octanol-water partition coefficient P (or its logarithm \log P) serves as a fundamental descriptor of a molecule's , quantifying its relative affinity for a nonpolar organic versus an aqueous . A high \log P value, typically greater than , indicates that the solute predominantly partitions into the octanol , reflecting dominant hydrophobic interactions that favor nonpolar environments. Conversely, a low \log P value, often below , signifies hydrophilic behavior, where the molecule prefers the water due to stronger polar interactions with water molecules. This partitioning arises from differences in intermolecular forces between the two solvents. In water, polar solutes engage in extensive bonding and electrostatic interactions, leading to favorable through ordered structures around hydrophilic groups. Octanol, being less polar with a longer chain, promotes van der Waals dispersion forces and weaker bonding (facilitated by its hydroxyl group and residual of about 2.3 M at ), which better accommodate nonpolar moieties. The balance of these forces—hydrophobic effects driving exclusion from , alongside energies—determines the of transfer, with each additional in homologous series increasing P by approximately a factor of 3 due to enhanced van der Waals contributions. Octanol's amphiphilic structure, combining a polar head and nonpolar tail, positions it as a biomimetic model for bilayers and , capturing essential aspects of partitioning without the complexity of actual biological systems. This analogy stems from octanol's ability to simulate the hydrophobic core and interfacial hydrogen-bonding sites of bilayers, allowing \log P to reflect molecular tendencies toward integration. The coefficient is dimensionless, expressed as the ratio of concentrations at , with \log P values commonly spanning -3 for highly hydrophilic compounds (e.g., simple polyols) to +7 for very lipophilic ones (e.g., long-chain hydrocarbons), encompassing most environmentally and biologically relevant molecules.

Historical Development

Early Concepts

The origins of the octanol-water partition coefficient trace back to 19th-century investigations into solute distribution between immiscible solvents, formalized by Walther Nernst's in 1891. Nernst demonstrated that, at constant , a solute distributes itself between two non-miscible phases such that the of its concentrations in the two phases remains constant, assuming the solute is in the same molecular form in both. This principle, illustrated through experiments with partitioning between and while accounting for factors like dimerization and ionization, provided the thermodynamic basis for partitioning and influenced subsequent studies on and phase transfer. Early 20th-century research extended these ideas to biological contexts, particularly in understanding narcosis and interactions. In 1899, Hans Horst Meyer examined the effects of organic compounds on tadpoles, finding that their potency correlated directly with their partitioning into from water; independently, Charles Ernest Overton reached similar conclusions in 1901. This work, part of the emerging lipoid theory of narcosis, highlighted how oil-water partition coefficients could predict a compound's ability to cross biological barriers, with higher coefficients indicating greater permeability and . Before octanol became prominent, early lipophilicity assessments relied on analog solvents to model hydrophobic environments. was frequently used as a proxy for biological in narcosis studies by Meyer and contemporaries, offering a natural, complex phase that approximated cellular membranes. , meanwhile, served in distribution experiments for solutes like carboxylic acids, revealing wide ranges in partition behavior influenced by and interactions. In the 1930s, Runar Collander and Harald Bärlund conducted experiments on the alga Chara ceratophylla, measuring the penetration rates of non-electrolytes and correlating these with their distribution coefficients across various solvent pairs, such as olive oil-water and chloroform-water. In 1951, Collander further investigated the partitioning of organic compounds between higher alcohols, including n-octanol, and water, demonstrating that n-octanol-water partitioning effectively predicted passive diffusion through biological membranes due to its amphiphilic nature mimicking lipid interfaces.

Standardization and Evolution

In the mid-20th century, the octanol-water system emerged as the preferred model for assessing in , largely due to the pioneering efforts of Corwin Hansch and Toshio Fujita. Their 1964 publication introduced the hydrophobic substituent constant π, calculated from differences in n-octanol-water partition coefficients (log P), which enabled systematic quantitative structure-activity relationship (QSAR) analyses for correlating with . This adoption of n-octanol over earlier solvents like or standardized the measurement, as its amphiphilic nature—featuring a polar hydroxyl group and a nonpolar chain—better simulated interactions with biological membranes. International accelerated in the late through regulatory frameworks. The adopted Test Guideline 107 in 1981, detailing the shake-flask method for direct measurement of log P values typically between -2 and 4, ensuring reproducible experimental conditions across laboratories. This was expanded in 1989 with Test Guideline 117, which incorporated (HPLC) for estimating log P up to 6, offering greater efficiency for volatile or low-solubility compounds while maintaining comparability to shake-flask results. These guidelines established n-octanol-water partitioning as a core physicochemical parameter in assessments. The 1970s and 1980s saw refinements addressing limitations for ionizable compounds, where partitioning varies with due to states. Albert Leo, Corwin Hansch, and David Elkins formalized the distinction in their 1971 review, defining the distribution coefficient (log D) as the pH-dependent ratio of total analyte concentrations in octanol and aqueous phases, contrasting it with log P for the neutral form. This evolution improved QSAR applicability in , recognizing that physiological (around 7.4) often results in mixed , thus log D provides a more realistic metric for predictions. Recent decades have focused on methodological enhancements and . The introduced high-throughput shake-flask and HPLC variants, automating measurements for hundreds of compounds daily to support rapid screening, with accuracies comparable to traditional methods. In the , efforts have shifted toward eco-friendly alternatives to n-octanol, such as hydrophobic deep eutectic solvents and biomimetic systems like liposomes or membranes, which reduce use while offering partitioning data more aligned with cellular environments.

Experimental Determination

Methods of Measurement

The primary experimental methods for determining the octanol-water partition coefficient (log Pow) rely on achieving between the two immiscible phases and quantifying the solute concentrations in each. These techniques are standardized by organizations such as the to ensure reproducibility and accuracy for substances spanning a range of hydrophobicities. The choice of method depends on the compound's , , and log Pow value, with direct partitioning approaches preferred for validation against computational estimates. The shake-flask method, outlined in Test Guideline 107, is the traditional reference technique for measuring Pow values typically between -2 and 4. It involves preparing mutually saturated n-octanol and phases, adding the test substance to the mixture in a or centrifuge tube, and shaking vigorously (e.g., 100 oscillations over 5 minutes at 20-25°C) to reach , followed by phase separation via . Concentrations are then analyzed in both phases using techniques such as UV-Vis , (GC), or (HPLC), ensuring minimal cross-contamination during sampling. The is calculated as the ratio of the solute concentration in the octanol phase ([C]oct) to that in the phase ([C]water), expressed as Pow = ([C]oct/[C]water). Duplicate or triplicate runs with varying phase volume ratios are performed to verify consistency, with results within ±0.3 units considered acceptable. For compounds with log Pow values between 0 and 6, the HPLC correlation method ( Test Guideline 117) provides an efficient alternative by indirectly estimating log Pow through chromatographic retention behavior. The test substance is injected into a reverse-phase HPLC system equipped with a C8 or C18 silica column and a mobile phase of methanol-water (e.g., 75:25 v/v), using UV detection at 210 nm. Retention times (tR) are measured relative to the dead time (t0), yielding the capacity factor k = (tR - t0)/t0. This is correlated via (log Pow = a + b log k) against 6-10 standards with known log Pow values that bracket the test compound's expected range, such as (log Pow = 2.38) and (log Pow = 6.19). The method requires a of at least 0.9 and is unsuitable for strongly ionizable or surface-active substances without pH buffering. For highly hydrophobic compounds with low water solubility (log Pow > 5), the slow-stirring (OECD Test Guideline 123) minimizes artifacts from formation by using gentle agitation in a large-volume reactor. Mutually saturated n-octanol and phases (e.g., 1:10 volume ratio) are combined with the test substance dissolved in octanol, then stirred at low speed (creating a 0.5-2.5 cm vortex) for up to 144 hours at 25°C until is confirmed by stable concentration ratios over time. Samples from each phase are analyzed by HPLC or , with the particularly effective for substances like polychlorinated biphenyls where traditional shaking risks overestimation due to octanol microdroplets in . Similarly, the -column , as described in EPA OPPTS 830.7560 and TSCA §799.6756, employs a dynamic flow system for log Pow values of 1-6. A column packed with silanized coated in 1% (w/w) test substance in n-octanol is equilibrated with flowing at 1 mL/min for 15 minutes at 25°C; the effluent aqueous concentration is measured by HPLC or after extraction, allowing calculation from the phase ratio without direct phase separation. This approach suits volatile or low-solubility compounds by generating saturated solutions continuously. pH-dependent variants address ionizable compounds by measuring the distribution coefficient (log D), which accounts for speciation. These adaptations, often based on the shake-flask or slow-stirring methods, use buffered aqueous phases (e.g., phosphate buffer at 7.4) to maintain a specific , with partitioning and analysis proceeding as in the parent techniques. Log D is calculated analogously to log Pow but reflects the total (ionized + neutral) solute distribution, enabling assessment at physiological or environmental values; for instance, weak acids show decreased log D at high due to favoring the aqueous phase.

Sources of Error and Limitations

Experimental determination of the octanol-water partition coefficient (Kow) is susceptible to several common sources of error that can compromise accuracy. Emulsion formation, particularly during vigorous shaking in the shake-flask method, often results in small octanol droplets contaminating the aqueous phase, leading to overestimated water concentrations and underestimated log Kow values, especially for compounds with log Kow > 6. Incomplete equilibration poses another challenge, as achieving true partition equilibrium may require hours to days depending on the compound, with insufficient agitation time causing persistent inaccuracies in traditional shake-flask approaches, which become unreliable for log Kow > 4–5 due to microemulsion risks. Volatility losses of solutes during handling or analysis further contribute to errors, particularly for more volatile compounds, by reducing measured concentrations in both phases. To mitigate these issues, the slow-stirring method is recommended, as it minimizes emulsion formation and interfacial effects; inter-laboratory ring tests using this approach have demonstrated variability of less than 1 log unit across 15 labs. Certain compound classes present inherent limitations in Kow measurement. For ionizable species such as acids or bases, the is highly pH-dependent, as shifts the distribution toward the aqueous (log D < log P), with measurements requiring control at specific pH values to reflect neutral forms; for instance, log D can vary significantly across pH 1–13 for pharmaceuticals. Highly lipophilic compounds with log Kow > 5 suffer from extremely low aqueous (< 10−5 M), resulting in analytical challenges like detection limits and difficulties, often necessitating alternative methods like generator columns but still yielding higher uncertainties. introduce additional interference due to their amphiphilic nature, promoting aggregation at interfaces and emulsification that obscures true partitioning, rendering standard shake-flask measurements impractical without modifications like sample evaporation and redissolution to eliminate octanol effects. Environmental factors like and also affect measurement reliability. Standard conditions specify 25°C to ensure comparability, but deviations alter Kow; for example, increasing from 25°C to 45°C decreases log Kow by approximately 0.24 units for , equating to a 2–3% change in Kow per °C due to enhanced solute in . Elevated , such as from (0.5–50 psu), can slightly increase log Kow by 0.1–0.2 units through the salting-out effect, though this is less pronounced than influences. Reproducibility remains a key limitation, with inter-laboratory variability often reaching up to 0.5 log units due to differences in protocols, equipment, and analyst expertise, as accepted under Test Guideline 117 for methods. Recent 2025 analyses of consolidated datasets, integrating multiple experimental and predictive methods for over 170 chemicals, have shown that combining at least five independent estimates reduces this variability to within 0.2–0.3 log units, enhancing overall accuracy for environmental and regulatory applications.

Computational Estimation

Empirical and QSAR Methods

Empirical methods for estimating the octanol-water partition coefficient (log P) rely on additive contributions from molecular fragments or substituents, derived from experimental data on known compounds. These approaches assume that the overall can be calculated by summing the hydrophobic or hydrophilic contributions of individual structural units, often with corrections for intramolecular interactions. One seminal fragment-additive , introduced by , Hansch, and Elkins, calculates log P as the sum of fragment values plus correction factors: \log P = \sum f_i + \sum F_j where f_i represents the contribution of each fragment and F_j accounts for electronic or that alter additivity. This , foundational to tools like CLOGP developed by Hansch and in 1971, uses atomic and group constants calibrated against a database of measured log P values to predict for new molecules. Quantitative structure-activity relationship (QSAR) models extend these empirical techniques by employing linear free energy relationships (LFER) to correlate log P with molecular descriptors. Hansch analysis, a of QSAR, applies to relate biological or physicochemical properties to parameters such as log P, substituent constants (π for hydrophobicity), and steric factors (Es). For log P estimation specifically, these models often regress against descriptors like molecular weight and , yielding equations of the form \log P = a \cdot MW + b \cdot PSA + c, where coefficients are fitted to training data. This approach, pioneered in the 1960s and refined in subsequent works, enables predictive modeling by capturing how structural variations influence partitioning behavior. Commercial software implements these empirical and QSAR methods for practical log P prediction. ACD/LogP, for instance, employs a fragment-based classic algorithm trained on over 12,000 experimental values, supplemented by the GALAS method that adjusts predictions based on structural similarity to a larger dataset of more than 22,000 compounds; it achieves accuracy within approximately 0.5 log units for compounds in its training space. Similarly, the KOWWIN module in EPA's EPI Suite uses a fragment-constant approach, assigning coefficients to atomic fragments and larger groups derived from a training set of around 10,000 compounds, with reported root-mean-square errors of about 0.4 log units on validation data. These tools prioritize speed and broad applicability for screening in drug design and environmental assessments. Despite their utility, empirical and QSAR methods have notable limitations, particularly for compounds with scaffolds or underrepresented functional groups in sets, where predictions can deviate significantly due to unaccounted interactions. Flexible molecules often face overestimation of log P, as additive models struggle to capture conformational effects that influence . Validation against experimental data remains essential to assess reliability for specific chemical classes.

Advanced Computational Approaches

Advanced computational approaches for predicting the octanol-water partition coefficient (log P) leverage quantum mechanical calculations, molecular dynamics simulations, and techniques to achieve higher accuracy and mechanistic insight compared to empirical methods. These methods focus on computations of free energies or data-driven models trained on extensive datasets, enabling predictions for diverse chemical structures without relying on predefined descriptors. Quantum mechanical methods, such as the Conductor-like Screening Model for Realistic Solvents (COSMO-RS), provide a predictive framework for solvation free energies in octanol and water, directly yielding the free energy of transfer. In COSMO-RS, the solvation free energy is computed from quantum chemical surface charge densities, allowing for the estimation of partition coefficients through thermodynamic cycles. The key relation is the free energy of transfer from water to octanol, given by \Delta G_{\text{transfer}} = \Delta G_{\text{octanol}} - \Delta G_{\text{water}}, where log P = -\Delta G_{\text{transfer}} / (2.303 RT), with typical root-mean-square deviations around 0.3 log units for neutral compounds. This approach excels for ionic and polar species, where continuum models capture electrostatic and dispersion interactions effectively. For instance, COSMO-RS has been applied to predict log P for electrolytes, accounting for ion-specific effects in mixed solvents. (MD) simulations employ (FEP) to compute partitioning in explicit solvent environments, offering atomistic detail on solute-solvent interactions. In FEP-based MD, alchemical transformations gradually mutate the solute between water and octanol phases, yielding solvation free energies via thermodynamic integration or . These simulations use force fields like OPLS-AA or CHARMM to model explicit octanol (often water-saturated) and water boxes, achieving accuracies of 0.5-1.0 log units for small organics in blind challenges. Such methods are computationally intensive but valuable for validating ML models or studying conformational effects in flexible molecules. Machine learning techniques have advanced log P prediction through deep neural networks (DNNs) and ensemble methods like random forests, trained on large datasets of experimental values. DNNs, utilizing graph representations of molecular structures, capture nonlinear relationships in , as demonstrated in a 2021 study where models trained on over 10,000 compounds achieved mean absolute errors below 0.4 log units on held-out data. Random forests, employing descriptors derived from SMILES strings (e.g., molecular fingerprints or graph features), provide interpretable predictions with similar accuracy, often outperforming traditional QSAR for diverse datasets by handling high-dimensional inputs robustly. Recent developments include consolidated datasets that integrate multiple experimental sources and hybrid quantum mechanical/ approaches that combine QM-derived features with neural networks to improve predictions while retaining physical interpretability. As of , commercial tools like ACD/ have expanded their training sets, enhancing overall accuracy for log P calculations.

Applications

In Drug Discovery and Pharmacology

The octanol-water partition coefficient, denoted as log P, is integral to for predicting absorption, distribution, metabolism, and excretion (ADME) profiles, particularly in optimizing for oral . , formulated to guide the selection of drug-like molecules, specifies that compounds with a log P exceeding 5 are prone to poor due to excessive , while values below 1 may compromise membrane permeability; an optimal range of 1 to 5 balances solubility and passive diffusion. This guideline correlates with permeability assessments in parallel artificial membrane permeability assay (PAMPA) models, where log P values in this range predict effective , with permeability increasing linearly up to log P ≈ 3 before plateauing due to solubility limitations. In pharmacology targeting the central nervous system (CNS), log P ≈ 2 facilitates optimal blood-brain barrier (BBB) penetration by promoting passive diffusion while limiting non-specific tissue binding, as evidenced by quantitative structure-activity relationship (QSAR) analyses of CNS-penetrant drugs. This value also mitigates efflux by P-glycoprotein (P-gp), an ATP-binding cassette transporter that actively pumps out highly lipophilic substrates (log P > 3), thereby enhancing brain exposure for therapeutic efficacy. Log P informs toxicity prediction models, where elevated values (>4) heighten the risk of human ether-à-go-go-related gene (hERG) potassium channel inhibition, potentially causing QT interval prolongation and arrhythmias, as integrated into physicochemical QSAR frameworks for early risk assessment. Similarly, log P is a key descriptor in QSAR models for metabolic stability, with moderate values (1-4) favoring hepatic clearance via cytochrome P450 enzymes without excessive phase I metabolism liability. In statin development, atorvastatin (log P ≈ 6.4) exemplifies log P considerations, where its high lipophilicity required formulation strategies to improve solubility and oral absorption, reducing off-target effects compared to more hydrophilic analogs like pravastatin.

In Environmental and Toxicological Studies

The octanol-water partition coefficient plays a crucial role in for assessing the fate and transport of organic chemicals, particularly their potential for in aquatic ecosystems. The factor (BCF), which measures the accumulation of a substance from into an organism's tissues, correlates strongly with log P values through models like the Gobas bioaccumulation model. For aquatic organisms, empirical relationships indicate that log BCF ≈ 0.8 log P - 0.4 for moderately hydrophobic chemicals, reflecting how lipophilic compounds partition preferentially into lipid-rich tissues, thereby increasing their concentration relative to surrounding ; however, for log P > 6, may plateau due to limits and reduced uptake. This relationship highlights the risk of trophic magnification in food chains for moderately hydrophobic substances. In and environments, log P informs predictions of chemical partitioning between and solid phases, aiding evaluations of and long-term persistence. The Karickhoff relates the organic carbon- partition coefficient (Koc) to log P as log Koc ≈ log P - 0.21 (or Koc ≈ 0.63 Kow), indicating that higher log P values enhance to in soils, reducing to but promoting retention and potential exposure via ingestion or . This behavior is essential for modeling contaminant distribution in terrestrial systems and assessing remediation needs. Regulatory frameworks leverage log P thresholds to screen for persistent, bioaccumulative, and toxic (PBT) substances. Under the European REACH regulation (Annex XIII), a log P > 3 serves as a screening criterion for bioaccumulative (B; BCF ≥ 2000) potential, while log P > 4.5 indicates potential for very bioaccumulative (vB; BCF ≥ 5000), triggering further PBT evaluation if persistence and toxicity criteria are also met when experimental BCF data are unavailable. Similarly, U.S. EPA guidelines identify chemicals with log P > 3 as having bioaccumulation potential in PBT assessments, guiding prioritization for risk management and restrictions on releases. Illustrative examples include persistent pesticides and industrial chemicals like and polychlorinated biphenyls (PCBs), which demonstrate elevated environmental risks due to high log P values. has a log P of 6.91, contributing to its strong (BCF > 10,000 in ) and decades-long persistence in soils and sediments, leading to widespread ecotoxicological impacts. PCBs, with log P values ranging from 5.7 for tetra-chlorinated congeners to 7.0 for hexa-chlorinated ones, similarly exhibit high persistence and , magnifying toxicity through aquatic food webs and prompting global regulatory bans under the Stockholm Convention.

Example Values and Databases

Selected Examples

The octanol-water partition coefficient, expressed as log P, varies widely across chemical classes, reflecting differences in hydrophobicity and molecular structure. Representative examples illustrate how log P values range from negative for highly polar compounds to positive and high for nonpolar or fluorinated ones, providing insight into their partitioning behavior.
CompoundChemical Classlog P ValueSource
Alcohol-0.31Hansch et al. (1995)
PhenolPhenol (pollutant)1.46Hansch et al. (1995)
Aromatic hydrocarbon2.13Hansch et al. (1995)
AspirinPharmaceutical1.19Hansch et al. (1995)
IbuprofenPharmaceutical3.97Hansch et al. (1995)
Polycyclic aromatic3.30Hansch et al. (1995)
Organochlorine pollutant6.91de Bruijn et al. (1989)
PerfluorooctanePerfluoroalkane8.09Computed by XLogP3 (PubChem)
These values highlight diversity across classes: low for polar pharmaceuticals and pollutants like ethanol and phenol, moderate for aromatics, and high for persistent pollutants and fluorocarbons like and perfluorooctane. Trends in log P values demonstrate systematic effects of structure. In alkanes, log P increases with chain length, typically by approximately 0.5 units per additional CH₂ group, as seen in the progression from (log P ≈ 2.9) to (log P ≈ 5.2), due to enhanced nonpolar surface area. Functional groups modulate this: introduction of a hydroxyl (-) group decreases log P by about 1 unit by increasing hydrogen bonding with , as evident in comparing (log P 2.13) to phenol (log P 1.46). Comparisons between measured and predicted log P values validate estimation methods. For aspirin, the experimental value of 1.19 aligns closely with the CLOGP prediction of 1.19, demonstrating high accuracy for this tool in aromatic systems with polar substituents. Such concordance underscores the utility of computational approaches for screening, though discrepancies can arise in complex molecules like perfluorooctane, where experimental measurement is challenging due to extreme hydrophobicity.

Available Databases

Public databases provide extensive access to octanol-water partition coefficient (log P or log Kow) data, supporting research in chemistry, , and . , maintained by the , hosts millions of compound entries with both experimental and predicted log P values derived from various assays and computational models. The U.S. Environmental Protection Agency's EPI Suite offers estimation tools like KOWWIN, which incorporate curated datasets of measured log P for over 14,000 chemicals to train and validate predictions of hydrophobicity. Specialized resources focus on high-quality, measured data for quantitative structure-activity relationship (QSAR) analysis. The Hansch-Leo dataset, compiled by Corwin Hansch and Albert Leo in their seminal work on QSAR fundamentals, serves as a classic training set with log P data for nearly 35,000 organic compounds, emphasizing structure-lipophilicity correlations. PhysProp, developed by Syracuse Research Corporation, is a comprehensive database containing experimental physical properties, including over 13,500 measured log P values for more than 41,000 chemicals sourced from peer-reviewed literature. A notable recent development is the 2025 consolidated Kow published by , which harmonizes experimental log Kow data from multiple sources to reduce measurement variability, achieving consistency within 0.2 log units for robust hydrophobicity assessments. These databases are invaluable for training models, validating computational predictions, and benchmarking experimental methods in log P studies. However, limitations persist, such as incomplete coverage for emerging chemicals like novel or complex mixtures, necessitating ongoing data curation efforts.

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