Fact-checked by Grok 2 weeks ago

Bioconcentration

Bioconcentration is the net accumulation of a chemical in the tissues of an aquatic organism due to uptake directly from the surrounding water across respiratory surfaces such as gills or external body surfaces, excluding contributions from dietary ingestion or other routes.^[1] This process is quantified by the bioconcentration factor (BCF), defined as the ratio of the chemical's steady-state concentration in the organism to its concentration in the water, typically expressed on a wet-weight basis for fish.^[2] Bioconcentration is distinct from broader bioaccumulation, which incorporates dietary uptake, and serves as a critical metric in environmental toxicology for evaluating the persistence and potential ecological risks of organic pollutants like pesticides and industrial chemicals in aquatic ecosystems.^[3] High BCF values, often correlated with hydrophobicity measured by the octanol-water partition coefficient (K_OW), indicate a substance's tendency to partition into lipid-rich tissues, influencing regulatory thresholds for chemical safety assessments.^[4] Empirical measurements of BCF are conducted under controlled laboratory conditions to ensure steady-state equilibrium, though variability arises from factors such as organism species, exposure duration, and chemical properties like metabolism rates.^[5]

Definition and Mechanisms

Core Definition and Distinctions

Bioconcentration is the net accumulation of a dissolved substance in the tissues of an aquatic organism resulting from direct uptake from the surrounding water through non-dietary pathways, such as respiration across gills or dermal absorption, until a steady state is reached where the rates of uptake and elimination are equal.^[5]^[6] This process is distinct in its reliance on passive diffusion driven by concentration gradients and the physicochemical properties of the substance, without involving ingestion of contaminated food.^[7] The bioconcentration factor (BCF) quantifies this accumulation as the ratio of the substance's concentration in the organism's tissue (typically on a wet weight basis) to its concentration in the water at steady-state equilibrium, expressed in liters per kilogram (L/kg).^[8] Empirical determination of BCF involves laboratory exposure tests under controlled conditions, measuring tissue concentrations after sufficient time for equilibrium, often following guidelines like OECD Test No. 305 for flow-through fish tests. Bioconcentration differs from bioaccumulation, which encompasses net uptake from all exposure routes including dietary ingestion, and from biomagnification, which describes the progressive increase in substance concentration across trophic levels in a food web due to efficient trophic transfer exceeding elimination rates.^[2]^[5] These distinctions emphasize bioconcentration's specific focus on waterborne exposure, avoiding conflation with broader ecological dynamics that may amplify concentrations through food chains.^[7]

Uptake and Elimination Processes

In aquatic organisms, particularly fish, the uptake of hydrophobic organic chemicals during bioconcentration primarily occurs via passive diffusion across permeable surfaces such as gills and skin, governed by thermodynamic fugacity gradients that drive net flux from water into lipid-rich tissues.^[9] This process is facilitated by the chemical's partitioning behavior, with uptake rates increasing for compounds exhibiting log Kow values exceeding 3, where the aqueous diffusion boundary layer at the gill epithelium imposes the principal resistance to mass transfer rather than membrane permeability.^[10] Experimental measurements in fish confirm that ventilatory water flow and gill surface area modulate this influx, yielding uptake clearance rates on the order of 100-1000 liters per kilogram of fish per day for moderately hydrophobic substances under steady exposure conditions.^[11] Elimination pathways counterbalance uptake through diffusive efflux across gills back to water, driven by the reverse fugacity gradient once tissue concentrations exceed those in the medium, alongside biotransformation via hepatic enzymes such as cytochrome P450 isoforms that catalyze oxidative phase I metabolism to more polar derivatives.^[12] Fecal egestion contributes by excreting unmetabolized chemical or conjugates from dietary or biliary sources, while non-metabolic gill ventilation sustains baseline depuration for non-polar compounds with low metabolic clearance. Kinetic studies quantify these as composite elimination rates, typically ranging from 0.01 to 0.1 per day in fish, reflecting the dominance of respiratory exchange for persistent hydrophobics and enzymatic transformation for susceptible substrates.^[13] Steady-state bioconcentration emerges from mass balance when uptake flux equals total elimination flux, stabilizing internal concentrations as a function of the underlying kinetic partitioning, as demonstrated in laboratory assays with rainbow trout exposed to controlled aqueous concentrations of chlorinated hydrocarbons, where equilibrium is attained within days to weeks depending on chemical persistence.^[14] This equilibrium underscores the causal role of differential transport kinetics in dictating net accumulation, independent of organismal growth or external perturbations in validated static-renewal protocols.^[15]

Historical Development

Early Observations in Aquatic Toxicology

Initial empirical evidence of bioconcentration arose in the 1940s following the commercial introduction of DDT and related organochlorine pesticides for insect control, with laboratory bioassays demonstrating uptake into fish tissues at levels far exceeding ambient water concentrations. Early investigations, including those prompted by mosquito abatement programs, documented acute toxicity and residue persistence in non-target species such as trout and minnows, where DDT partitioned into lipids due to its hydrophobic nature, yielding tissue burdens orders of magnitude higher than in exposure media. These findings stemmed from direct measurements via rudimentary extraction methods, revealing causal mechanisms tied to passive diffusion across gills and epithelial surfaces rather than active transport.^[16]^[17] By the 1950s, field surveys expanded on these observations, particularly in agricultural runoff-impacted waters, where organochlorines like dieldrin and endrin showed similar enrichment patterns in benthic and pelagic fish. U.S. Fish and Wildlife Service (then Bureau of Sport Fisheries and Wildlife) residue analyses confirmed that lipid content correlated positively with accumulation, as evidenced in studies of insecticide-sprayed watersheds, without presuming universal ecological collapse but noting selective impacts on fatty tissues. Quantification via early chromatographic techniques enabled initial estimates of concentration ratios, often exceeding 1000:1 for water-to-fish, grounded in verifiable sampling from sites like eastern U.S. streams post-application.^[18]^[19] Such pre-regulatory data informed recognition of bioconcentration as a physicochemical process driven by equilibrium partitioning, distinct from dietary magnification, with empirical validation from controlled exposures showing steady-state tissue levels proportional to exposure duration and chemical solubility. Rachel Carson's 1962 Silent Spring synthesized these federal and state reports—drawing on U.S. Fish and Wildlife Service monitoring of pesticide residues in aquatic biota—to highlight disruptions in food webs, yet the underlying studies prioritized factual residue mapping over advocacy, reflecting measured scientific inquiry into causal exposure-response dynamics.^[20]^[17]

Key Studies and Conceptual Advances

In 1974, Neely et al. derived one of the earliest quantitative relationships for predicting bioconcentration factors (BCF) in fish, establishing a linear correlation between the logarithm of the octanol-water partition coefficient (log K_OW) and log BCF based on controlled aqueous exposure experiments with nonionic organic chemicals, yielding the approximation log BCF = log K_OW - 1.32.^[21] This empirical model underscored the causal influence of molecular hydrophobicity on passive uptake via gill diffusion, providing a foundational tool for assessing accumulation potential without dietary contributions.^[21] The U.S. Environmental Protection Agency subsequently integrated BCF metrics into pesticide regulatory evaluations during the 1970s, leveraging such correlations to evaluate risks of environmental persistence and trophic transfer in aquatic ecosystems.^[22] During the 1980s, conceptual advances incorporated thermodynamic principles to refine bioconcentration predictions. Mackay (1982) applied fugacity-based modeling to bioconcentration processes, treating organisms as compartments in equilibrium with surrounding water and emphasizing steady-state partitioning driven by chemical activity gradients rather than simplistic partition coefficients alone. This approach facilitated integration of bioconcentration into broader multimedia fate models, accounting for elimination rates and inter-media transfers, and improved accuracy for volatile or metabolizable compounds beyond the limitations of static correlations. In the 2000s, large-scale empirical validations strengthened causal understandings of bioconcentration dynamics. Arnot and Gobas (2006) reviewed 5,317 measured BCF values and 1,656 bioaccumulation factors from 392 scientific sources and databases, confirming the strong linkage between lipophilicity (via K_OW) and accumulation for chemicals with log K_OW below 6, while demonstrating systematic underprediction by equilibrium models for superhydrophobic substances due to restricted gill permeation and enhanced biotransformation.^[4] Their analysis highlighted the necessity of kinetic parameters—such as uptake clearance and depuration rates—in mechanistic descriptions, reducing reliance on unverified assumptions and enabling more robust regulatory thresholds for persistent organics.^[4]

Influencing Factors

Biological Variables

Bioconcentration factors (BCFs) in aquatic organisms vary significantly due to inherent biological traits, with empirical studies demonstrating that lipid content, body size, and metabolic rates are primary determinants of uptake and elimination kinetics for hydrophobic substances. Interspecies comparisons reveal BCF ranges spanning orders of magnitude; for instance, lipid-normalized BCFs for persistent organic pollutants can differ by factors of 10 or more between fatty and lean fish species under standardized conditions. This variability underscores limitations in assuming uniform accumulation risks across taxa, as standardized tests like OECD Guideline 305 often normalize to 5% whole-body lipid to facilitate comparisons, yet real-world physiological differences persist.^[23]^[24] Lipid content exerts a dominant influence, as hydrophobic compounds partition preferentially into non-polar lipids, leading to higher steady-state concentrations in lipid-rich tissues. Wet-weight BCFs increase linearly with lipid percentage; a study of eight fish species exposed to 1,2,4-trichlorobenzene reported a significant positive correlation (r = 0.85, p < 0.05) between whole-body lipid levels (ranging 3-15%) and BCF values up to 10-fold higher in fatty species. Salmonids, with muscle lipids often exceeding 10% (e.g., Atlantic salmon at 12-18% in mature individuals), exhibit elevated BCFs for lipophiles like PCBs compared to lean species such as cod (<1% lipid), where normalized values align closer to 5% standards but unadjusted rates reflect lower accumulation. OECD protocols mandate lipid measurement and normalization to mitigate this, yet underscore that unadjusted BCFs in high-lipid organisms overestimate risks if not contextualized.^[25]^[23] Body size inversely affects BCF through surface-to-volume ratios and gill ventilation efficiency, with smaller organisms generally achieving higher uptake rates relative to elimination. Empirical data from invertebrates like amphipods show metal BCFs declining with body length (e.g., Cd BCF halved from 5 mm to 15 mm individuals), attributable to reduced permeable surface area per unit mass. In fish, allometric scaling models predict BCF decreases with mass^{0.25}, as larger individuals dilute uptake via greater biomass and slower relative growth; OECD 305 tests using juveniles (e.g., 1-10 g rainbow trout) yield higher BCFs than adults, challenging extrapolations to wild populations spanning size classes.^[26]^[27] Metabolic rates modulate net accumulation by enhancing biotransformation and excretion, particularly for metabolizable compounds. Faster basal metabolism, as in smaller or warmer-adapted species, accelerates elimination constants (k_2), reducing steady-state BCFs; quantitative structure-activity analyses link higher cytochrome P450 activity to 20-50% lower BCFs for phase I substrates in active species versus sluggish ones. Growth-corrected kinetic BCFs in OECD studies account for this, revealing that metabolism dominates over uptake for log K_OW > 5 substances, with empirical depuration half-lives shortening from >100 days in low-metabolism fish to <30 days in high-rate taxa. Reproductive stages amplify effects, as vitellogenic females accrue lipids (up to 2-fold increase in ovaries), elevating BCFs during oogenesis; medaka exposed to nonylphenols showed gender-differentiated accumulation tied to estrogenic yolk production.^[28]^[29]^[30]

Chemical Properties

The octanol-water partition coefficient (K_OW), expressed as log K_OW, serves as a primary predictor of bioconcentration potential for non-polar organic chemicals, reflecting their lipophilicity and affinity for biological lipids over water. Quantitative structure-activity relationship (QSAR) models establish a positive correlation between log BCF and log K_OW, typically linear up to log K_OW values of 6–7, where BCF peaks before declining due to diminished aqueous solubility limiting bioavailability.^[31] For instance, chemicals with log K_OW in the range of 4.5–8 exhibit predicted BCF exceeding 5000 in fish, underscoring the range of 2–7 as optimal for elevated bioconcentration among hydrophobic neutrals.^[31] ^[32] Molecular weight influences bioconcentration kinetics, with compounds below 500 Da facilitating higher uptake rates via passive diffusion across gill epithelia, whereas larger molecules (>700 Da) encounter steric barriers reducing permeation efficiency.^[33] Low volatility, indicated by vapor pressures below 10⁻³ Pa, enhances bioconcentration by minimizing partitioning to the air phase and sustaining aqueous concentrations for organism exposure.^[5] High vapor pressures promote volatilization, effectively lowering the chemical's availability in water and thus suppressing measured BCF values.^[5] Ionizable organic compounds display pH-dependent bioconcentration, as speciation shifts toward charged forms diminish lipid partitioning and elevate elimination rates relative to neutral counterparts. Mechanistic models accounting for pH effects predict substantially reduced BCF when ionization predominates, with uptake kinetics governed by the neutral fraction's membrane permeability.^[34] Empirical observations confirm this attenuation, particularly for bases and acids where internal pH gradients exacerbate trapping of ionized species within organisms.^[35] While BCF values exceeding 500 are empirically associated with heightened bioaccumulation potential in screening assessments, such thresholds warrant caution and must integrate toxicity endpoints to discern genuine ecological hazards, as high BCF alone does not imply adverse effects.^[36] These chemical traits underpin QSAR estimations but require validation against experimental data to mitigate overgeneralizations regarding persistence or inherent risk.^[37]

Environmental Conditions

Temperature exerts a significant influence on bioconcentration factors (BCFs) primarily by modulating uptake and elimination rate constants in aquatic organisms. Higher temperatures generally accelerate both processes through increased metabolic activity and membrane permeability, but the net impact on BCF—defined as the ratio of these rates—varies by chemical and species. For non-polar organics like permethrin in amphipods, elevated temperatures (e.g., from 12°C to 22°C) enhanced biotransformation, resulting in lower steady-state BCFs despite faster uptake.^[38] Similarly, quantitative structure-activity relationship models incorporating temperature-dependent partitioning predict reduced BCFs for hydrophobic compounds at warmer conditions due to disproportionately higher elimination rates, aligning with observed Q10 coefficients often exceeding 2 for elimination versus 1-1.5 for uptake.^[39] Field data from temperate aquatic systems corroborate this context-dependency, where seasonal warming correlates with diminished accumulation of persistent pollutants in fish tissues.^[40] pH modulates BCFs for ionizable compounds by altering speciation and lipophilicity, with neutral forms exhibiting higher bioavailability via passive diffusion across lipid membranes. For weak acids such as pentachlorophenol, BCFs in goldfish declined from approximately 10^4 at pH 6 to 10^2 at pH 8, reflecting ionization that traps the charged species in water and reduces uptake.^[41] Sulfadiazine, a sulfonamide antibiotic, showed analogous trends in zebrafish, with BCFs dropping from 50 (dry weight basis) at pH 6 to 36 at pH 8.5, linked to protonation enhancing octanol-water partitioning.^[42] This pH-dependence underscores causal speciation effects, as modeled by the fraction neutral (f_n) correlating directly with log BCF for bases and inversely for acids, explaining variability in lab versus field measurements where natural pH gradients (e.g., 6-9 in freshwater systems) amplify discrepancies.^[43] Salinity influences BCFs through osmotic adjustments affecting gill ventilation and chemical diffusion, often reducing accumulation in euryhaline species at higher levels. In blackrock fish exposed to perfluorinated compounds, BCFs for perfluorooctanesulfonate increased with salinity from 10 to 34 psu, attributed to decreased uptake and elimination rates at lower salinity delaying equilibrium.^[44] Conversely, for permethrin in resistant amphipods, salinity elevations (15-30 psu) lowered BCFs by enhancing biotransformation efficiency.^[38] These patterns highlight salinity's role in bioavailability, with marine conditions typically yielding 2-5 fold higher BCFs for fluorinated surfactants than freshwater analogs due to reduced osmotic stress on epithelial barriers.^[45] Dissolved organic matter (DOM), including humic substances, attenuates bioconcentration by sorbing hydrophobic contaminants, thereby lowering the freely dissolved aqueous concentration available for uptake. Meta-analyses of lab studies report DOM-induced BCF reductions of 2-98% for organics like polycyclic aromatic hydrocarbons, with effects proportional to DOM concentration and chemical log K_ow (e.g., >10 mg/L DOC diminishing bioavailability by complexation).^[46] Normalization via organic carbon partitioning (e.g., BCF_OC = BCF / (1 + K_doc * [DOC])) accounts for this sorption, as validated in fish exposures where Aldrich humic acid at 5 mg/L DOC halved pyrene accumulation.^[47] In humic-rich waters, such as boreal lakes (DOC 10-30 mg/L), this mechanism tempers field BCFs relative to sterile lab conditions, emphasizing DOM's causal role in underpredicting risks from unadjusted metrics.^[48] Water hardness, via cation-DOM interactions, further modulates this for metals; soft waters (low Ca/Mg) exhibit higher BCFs for copper in fish due to reduced precipitation and sorption competition.^[47]

Measurement and Calculation

Experimental Protocols

The primary standardized protocol for experimentally determining bioconcentration factors (BCFs) in fish is outlined in OECD Test Guideline 305, which specifies aqueous exposure tests using flow-through or semi-static (renewal) systems to maintain stable test substance concentrations in water.^[49] Juvenile fish, such as rainbow trout (Oncorhynchus mykiss) or common carp (Cyprinus carpio), are selected for their sensitivity and relevance, with groups of at least 40-80 individuals per concentration level divided into sampling subsets to allow destructive sampling without depleting replicates.^[23] Exposure concentrations are chosen to bracket environmentally relevant levels (typically 1-100 μg/L for organics), with a minimum of two concentrations tested, and systems designed to achieve >80% renewal of water volume daily in semi-static setups or continuous flow in flow-through aquaria to minimize variability and substance degradation.^[49] Water quality parameters (pH 6-8, temperature 12-23°C species-dependent, dissolved oxygen >60% saturation) are controlled and monitored, alongside regular verification of exposure concentrations via analytical methods such as gas chromatography-mass spectrometry (GC-MS) or high-performance liquid chromatography (HPLC).^[23] The uptake phase continues until steady-state bioconcentration is achieved—defined as no statistically significant increase in tissue concentration over at least two consecutive sampling intervals—or for a maximum of 42-56 days if steady-state is not reached, followed by a depuration phase in clean water to assess elimination kinetics.^[50] Fish are sampled destructively at predefined intervals (e.g., days 0, 3, 7, 14, 28, and end of uptake), with whole-body tissues homogenized, lipid-normalized where appropriate, and analyzed for test substance content using validated extraction and quantification techniques to ensure detection limits below 10% of the measured BCF.^[49] Controls include untreated and solvent (if used) groups to account for background uptake or adsorption losses to aquarium surfaces, verified by blank water and tissue analyses; growth dilution and mortality (<10%) are monitored, with tests invalidated if exceeding thresholds.^[23] A similar protocol is detailed in the U.S. EPA's OCSPP 850.1730 guideline, which emphasizes flow-through systems for volatile or unstable substances and requires at least 28 days uptake with equivalent analytical rigor.^[15] While ethical principles under the 3Rs framework (replacement, reduction, refinement) encourage minimizing fish numbers through optimized designs like internal benchmarking or minimized sampling, in vivo whole-organism tests remain essential for capturing integrated uptake, biotransformation, and elimination processes that in vitro assays (e.g., hepatocyte-based partitioning) cannot fully replicate.^[51] In vitro alternatives provide supportive data on intrinsic clearance but underestimate or overlook organism-level factors like growth and fecal egestion, necessitating validation against empirical fish data for regulatory BCF determinations.^[52]

Quantitative Metrics and Formulas

The bioconcentration factor (BCF) quantifies the extent of chemical accumulation in an aquatic organism from water exposure alone and is defined as the steady-state ratio of the chemical's concentration in the organism to that in water: BCF = \frac{C_b}{C_w}, where C_b is the concentration in the biota (typically fish tissue) and C_w is the concentration in water.^[53] Concentrations are standardized on a whole-body wet weight basis for the organism (μg/kg wet weight) and dissolved in water (μg/L), yielding units of L/kg wet weight to facilitate direct comparability across studies and species.^[15] This metric assumes equilibrium partitioning without dietary influences, emphasizing passive diffusion across respiratory surfaces like gills. From first-principles kinetics, the BCF derives from a one-compartment model treating the organism as a homogeneous reservoir exchanging chemical with water. The rate of change in biota concentration is given by \frac{dC_b}{dt} = k_u C_w - k_e C_b, where k_u (L/kg/day) is the uptake clearance rate constant reflecting gill ventilation and chemical permeability, and k_e (1/day) is the overall elimination rate constant encompassing diffusive efflux, biotransformation, and fecal egestion.^[53] At steady state (\frac{dC_b}{dt} = 0), uptake balances elimination, yielding BCF = \frac{k_u}{k_e}.^[15] This kinetic BCF (BCF_K) is preferred over direct ratio measurements for its independence from exposure duration and sensitivity to transient dynamics. In non-steady-state conditions, such as during juvenile growth phases common in laboratory tests, the elimination term expands to k_e = k_2 + k_E + k_M + k_G, incorporating biotransformation (k_2), egestion (k_E), metabolism (k_M), and growth dilution (k_G = \ln(2)/t_{1/2,growth} or specific growth rate).^[54] Growth dilution reduces measured BCF by partitioning chemical into new biomass, necessitating normalization: BCF_{SS} = \frac{k_u}{k_e - k_G} for time to steady state or growth-corrected BCF_{NG} = \frac{k_u}{k_e} \times e^{k_G t}, where t is exposure time, to estimate the no-growth equivalent and avoid underestimation for slowly equilibrating chemicals.^[54] For hydrophobic organics, lipid normalization enhances cross-study utility, as partitioning favors lipids: BCF_L (lipid weight basis, L/kg lipid) = BCF_{ww} \times \frac{100}{% lipid content}, often standardized to 5% lipid for fish to account for species variability (e.g., 3-10% typical range).^[53] This adjustment aligns with empirical observations that BCF correlates positively with lipid fraction up to saturation, though it assumes negligible water-phase solubility in tissue.^[13]

Regression and Empirical Estimations

Empirical regressions relating the logarithm of the bioconcentration factor (log BCF) to the logarithm of the octanol-water partition coefficient (log K_{OW}) offer practical approximations derived from extensive measured data in fish species. A seminal equation, developed by Veith et al. from bioconcentration experiments in fathead minnows (Pimephales promelas), is log BCF = 0.85 log K_{OW} - 0.70, exhibiting strong correlation (r ≈ 0.90-0.95) across diverse non-polar organic chemicals with log K_{OW} up to approximately 5-6.^[55] ^[56] This relationship assumes steady-state partitioning dominated by hydrophobicity, with predictions accurate within a factor of 4 for many compounds in validation sets.^[57] These regressions have been refined and validated using large empirical databases, such as the U.S. EPA's ECOTOX, which compiles over 4,000 fish BCF measurements from peer-reviewed studies, enabling derivation of fish-specific parameters with statistical confidence intervals typically spanning 0.5-1 log units.^[21] ^[58] For instance, updated slopes range from 0.76 to 0.85 and intercepts from -0.23 to -0.70, reflecting species variability like higher metabolism in predatory fish reducing apparent BCF. However, predictions often overestimate BCF for chemicals with log K_{OW} > 6, as hepatic metabolism and reduced gill uptake efficiency lower observed values below equilibrium estimates, with deviations up to an order of magnitude for metabolizable substrates like certain pesticides.^[59] Such adjustments, informed by empirical residuals, improve accuracy but highlight the need for compound-specific validation over broad screening.^[57]

Modeling Approaches

Fugacity-Based Models

Fugacity-based models for bioconcentration employ thermodynamic principles to describe the partitioning of chemicals between water and biological organisms, treating bioconcentration as the attainment of equal fugacity across phases. Fugacity, denoted as f, quantifies a chemical's tendency to escape from a phase, with concentration C related by C = [Z](/page/Z) \cdot f, where Z is the phase-specific fugacity capacity expressing the amount of chemical per unit fugacity. At equilibrium, f_\text{[water](/page/Water)} = f_\text{[organism](/page/Organism)}, yielding the bioconcentration factor \text{BCF} = C_\text{[organism](/page/Organism)} / C_\text{[water](/page/Water)} = Z_\text{[organism](/page/Organism)} / Z_\text{[water](/page/Water)}. For water, Z_\text{[water](/page/Water)} \approx 1 / [H](/page/H+), with [H](/page/H+) as the Henry's law constant (in Pa·m³/mol); for fish, Z_\text{[fish](/page/Fish)} incorporates lipid partitioning, approximated as Z_\text{[fish](/page/Fish)} = \rho_\text{[fish](/page/Fish)} \cdot (\phi_L \cdot K_\text{OW} + \phi_N \cdot K_\text{NW}) / [H](/page/H+), where \rho_\text{[fish](/page/Fish)} is fish density, \phi_L and \phi_N are lipid and non-lipid fractions, K_\text{OW} is the octanol-water partition coefficient, and K_\text{NW} accounts for non-lipid binding.^[60] Mackay's Level I model applies this framework in a closed system, assuming no net fluxes and pure equilibrium distribution based solely on Z values, often simplifying organisms to lipid-equivalent phases for hydrophobic compounds like PCBs. Level II extends to steady-state conditions with continuous chemical input (e.g., via ventilation from water) balanced by first-order loss processes such as metabolism or degradation, calculating fugacity as f = I / D, where I is input rate and D total degradation rate, adapted for biota compartments. These models, pioneered in the late 1970s by Donald Mackay, ground predictions in physical chemistry, enabling estimation of steady-state concentrations without empirical kinetic data.^[61]^[60] The approach excels in multimedia contexts, integrating bioconcentration with broader environmental partitioning by treating organisms as dynamic compartments linked via advective and diffusive flows, as in fugacity-based food web models for PCBs developed in 1997. However, static Level I/II formulations inherently limit handling of transient kinetics, such as uptake/elimination rates influenced by gill diffusion or growth dilution, necessitating extensions to time-variable or Level III/IV models for non-equilibrium scenarios. Empirical validation against measured BCFs for non-polar organics shows reasonable alignment for log K_\text{OW} < 6, but deviations arise for metabolizable or ionized compounds due to unaccounted reactive losses.^[60]

Equilibrium Partitioning Frameworks

Equilibrium partitioning frameworks model bioconcentration of hydrophobic organic compounds by assuming rapid thermodynamic equilibrium between the freely dissolved chemical concentration in water and its concentration in the organism's lipid phase, treating the process as passive partitioning akin to octanol-water distribution.^[1] This approach is particularly suited for screening non-polar, non-ionizing organics with log K_OW ≥ 4, where bioconcentration is driven by hydrophobicity and minimal biotransformation occurs. The baseline lipid-normalized bioconcentration factor (BCF_fLd) is equated to K_OW, reflecting the assumption that lipid-water partitioning mirrors octanol-water partitioning (K_LW ≈ K_OW).^[1] For wet-weight BCF estimation, the total concentration in biota (C_t^B) incorporates the organism's composition as fractions of water (f_w), lipid (f_R), and non-lipid organic matter (f_N): C_t^B = f_w C_w^fdB + f_R C_L + f_N C_N, yielding BCF_t^T ≈ f_R × K_OW + *x, where *x accounts for minor contributions from water and non-lipid phases (negligible for hydrophobic compounds).^[1] Fish lipid fractions (f_R) typically range from 0.03 to 0.07, with a default of 0.05 often applied, resulting in BCF ≈ 0.05 × K_OW for whole-body wet weight.^[1] Steady-state equilibrium is assumed after sufficient exposure (often >28 days for log K_OW ≥ 5), with validations against field data for polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) in systems like Lake Ontario showing over 90% of predictions within a factor of 5 of measured values.^[1] Extensions of these frameworks account for partitioning into non-lipid phases, such as structural proteins and carbohydrates, using phase-specific coefficients (K_NW for non-lipid organic matter, K_CW for carbohydrates), which become relevant for less hydrophobic or polar compounds but contribute minimally (<5%) to overall uptake in highly lipophilic organics. For instance, carbohydrate-water partition coefficients (K_CW) for PAHs range from 10^1.5 to 10^3.5, far below K_OW values exceeding 10⁵, underscoring lipid dominance. These models prioritize freely dissolved water concentrations, adjusting for sorption to dissolved organic carbon (K_DOC ≈ 0.08 × K_OW) and particulate organic carbon (K_POC ≈ K_OW) to refine bioavailability estimates.^[1]

Mechanistic and Machine Learning Models

Mechanistic models for bioconcentration predict chemical accumulation by simulating mass balance dynamics within aquatic organisms, incorporating physiological parameters such as gill ventilation rates and blood flow to describe uptake and elimination processes. The Gobas model (1993) exemplifies this approach, deriving bioconcentration factors from rate constants for gill diffusion, metabolic transformation, egestion, and growth dilution, applied to hydrophobic organics in food webs like Lake Ontario.^[62] These models solve time-dependent differential equations, such as the one-compartment form \frac{dC_B}{dt} = k_1 C_W - (k_2 + k_E + k_M + k_G) C_B, where C_B is biota concentration, C_W is water concentration, and k terms represent respective rates influenced by ventilation volume and perfusion limitations. Extensions of these frameworks account for species-specific traits, such as diffusive barriers in gills and gut for amphipods or perfusion-limited resistances in fish for surfactants, enabling predictions under non-equilibrium conditions.^[63]^[64] Validation against measured data confirms their utility for ionizable and hydrophobic compounds, though assumptions like steady-state partitioning may falter for slowly equilibrating chemicals.^[65] Machine learning models, emerging prominently in the 2020s, leverage algorithms like random forests and gradient boosting on molecular descriptors to forecast bioconcentration factors, often surpassing traditional quantitative structure-activity relationship (QSAR) regressions in handling chemical diversity and non-linearities. A 2025 study developed models using nine algorithms on descriptor datasets, achieving superior cross-validated performance metrics such as lower root mean square error compared to linear QSAR baselines.^[66] These approaches integrate vast empirical datasets for training, enabling predictions for untested compounds, yet require rigorous external validation against lab-derived BCFs to mitigate overfitting.^[67] Despite empirical gains, machine learning's opacity limits mechanistic insights, contrasting with interpretable physiological drivers in mass-balance simulations, and raises concerns over extrapolation to novel structures without causal grounding. Hybrid efforts combining ML with mechanistic priors are under exploration to balance predictive power and transparency.^[68]

Applications

Regulatory Frameworks and Criteria

Regulatory frameworks for bioconcentration primarily utilize bioconcentration factor (BCF) thresholds to identify substances warranting further scrutiny under persistent, bioaccumulative, and toxic (PBT) assessments. Under the European Union's REACH Regulation (Annex XIII), a substance satisfies the bioaccumulation (B) criterion if its BCF exceeds 2000 L/kg wet weight in fish, based on standardized testing protocols; values between 500 and 2000 L/kg indicate potential bioaccumulation, prompting weight-of-evidence evaluations that may include dietary bioaccumulation data or alternative metrics.^[69]^[70] The Organisation for Economic Co-operation and Development (OECD) aligns with this, defining bioaccumulative substances in PBT contexts as those with BCF greater than 2000, while very bioaccumulative (vB) status applies above 5000, emphasizing empirical fish bioconcentration tests over proxies when available.^[71] These criteria trigger integrated PBT assessments only when combined with persistence and toxicity endpoints, aiming to prioritize chemicals with demonstrated environmental partitioning potential. In the United States, the Environmental Protection Agency (EPA) integrates BCF data into ambient water quality criteria (AWQC) for protecting human health and aquatic life, particularly by estimating contaminant levels in edible fish tissues via bioconcentration from water.^[72] EPA guidelines for pesticide registration and chemical risk evaluation explicitly favor measured BCFs from laboratory studies—normalized to lipid content and steady-state conditions—over quantitative structure-activity relationship (QSAR) models, citing uncertainties in modeled predictions for hydrophobic compounds where experimental data better reflect uptake kinetics and elimination rates.^[36] When direct BCF measurements are unavailable, regulators screen using octanol-water partition coefficient (log K_OW) cutoffs, typically log K_OW greater than 3 as an indicator of potential bioaccumulation, though higher thresholds like 4.5 are applied for vB classification under REACH.^[70] Empirical analyses from 2023, drawing on compiled BCF datasets for thousands of organic chemicals, argue that elevating the general screening cutoff to log K_OW 4.5 across jurisdictions would maintain equivalent protection levels, as field and lab data show minimal bioconcentration increments between log K_OW 3 and 4.5 for many substances, potentially streamlining assessments without inflating risk estimates.^[70] This approach underscores reliance on causal partitioning data over conservative proxies to calibrate criteria against observed ecological concentrations.

Environmental Risk Assessment

Bioconcentration factors (BCFs) are integral to environmental risk assessments by enabling the estimation of chemical concentrations within aquatic organisms, thereby linking external water exposures to internal body burdens that drive toxicological effects. In standard frameworks, such as those under the European Medicines Agency (EMA) guidelines for medicinal products, BCFs derived from fish tests (e.g., OECD 305) are used when log K_ow ≥ 3, with values exceeding 100 L/kg triggering evaluations for secondary poisoning risks.^[73] Predicted no-effect concentrations (PNECs) for aquatic biota are typically calculated by dividing chronic no-observed-effect concentrations (NOECs) from toxicity tests by assessment factors (e.g., 10 for interspecies extrapolation), but BCFs adjust for amplified internal exposures in bioaccumulative substances, where risk quotients (RQs = predicted biota concentration / PNEC_biota) incorporate bioconcentration to assess ecosystem-level hazards.^[73] This integration ensures causal realism by focusing on mechanistically relevant dosimetry rather than solely external concentrations.^[74] For persistent pollutants like per- and polyfluoroalkyl substances (PFAS), BCFs serve as screening tools to identify bioaccumulation potentials that exacerbate long-term ecosystem risks, given PFAS persistence and trophic transfer. In deriving ecological screening values (ESVs), BCFs are applied in food-chain models to predict exposures for wildlife, with median values varying widely across PFAS homologues (e.g., higher for long-chain compounds like PFOS).^[75] However, bioavailability adjustments are essential, as factors such as dissolved organic carbon reduce PFAS uptake in invertebrates and fish, leading to overestimations in unadjusted BCFs; empirical studies show bioaccumulation declining with exposure concentration or sediment organic matter.^[76] These adjustments prevent undue conservatism in risk evaluations for sediment-associated exposures.^[77] Probabilistic assessments enhance BCF applications by propagating variability in empirical data—such as interspecies differences or test conditions—through Monte Carlo simulations to yield risk exceedance probabilities rather than binary RQs.^[78] Distributions of BCF values, often log-normal due to factors like lipid content and metabolism rates, allow quantification of uncertainty, revealing, for instance, elevated risks in sensitive taxa for substances with high-variability BCFs.^[78] This approach supports robust ecosystem hazard evaluations by distinguishing inherent variability from knowledge gaps, prioritizing monitoring for pollutants where probabilistic RQs indicate >10% chance of adverse effects.^[79]

Integration with Food Web Dynamics

Bioconcentration factor (BCF) serves as the foundational metric for aqueous-phase uptake in aquatic organisms, forming the baseline component of the broader bioaccumulation factor (BAF), which integrates both waterborne exposure and dietary assimilation across trophic levels.^[4] In food web dynamics, BAF exceeds BCF for persistent organic pollutants (POPs) when dietary routes dominate, as predators accumulate residues not only from ambient water but also from contaminated prey, leading to net trophic transfer without inherent magnification unless biomagnification factors (BMF) surpass unity.^[80] This distinction arises because bioconcentration alone reflects equilibrium partitioning driven by hydrophobicity (e.g., octanol-water partition coefficient, K_OW), whereas food web integration incorporates assimilation efficiencies, growth dilution, and elimination rates that modulate upward transfer.^[81] Food web models, such as those developed by Gobas, simulate these dynamics by parameterizing chemical fluxes between compartments, predicting BMF values greater than 1 for lipophilic substances with log K_OW exceeding approximately 4, where dietary lipid assimilation outpaces depuration. In these frameworks, BMF is calculated as the ratio of contaminant concentration in predator tissue to that in prey, normalized for lipid content and trophic position, revealing that biomagnification occurs primarily for chemicals resistant to metabolic transformation, as fecal egestion and biotransformation act as sinks reducing net uptake at higher levels.^[82] For instance, the Gobas aquatic model accounts for gill uptake (mirroring BCF) alongside gut absorption, yielding trophic magnification factors (TMF) that scale with persistence; chemicals with half-lives exceeding organism lifespan exhibit TMF >1, while readily metabolized ones approach or fall below 1.^[83] Empirical data from Great Lakes ecosystems illustrate this integration, where polychlorinated biphenyls (PCBs) demonstrate trophic amplification in fish food chains, with BMF values ranging from 1.5 to 3.5 for higher-chlorinated congeners in lake trout relative to forage fish like alewife.^[84] However, metabolism via aryl hydrocarbon receptor pathways reduces transfer for lower-chlorinated, more hydroxylated PCBs, capping observed TMF at around 2-4 across the chain despite baseline BCFs up to 10⁵ L/kg for individual congeners.^[85] These patterns underscore that while bioconcentration initiates residue buildup, food web dynamics determine propagation, with empirical discrepancies often attributable to variable lipid normalization and site-specific elimination rather than uniform magnification.^[86]

Toxicological Implications

Predictive Uses in Hazard Identification

Bioconcentration factor (BCF) predictions play a key role in hazard identification by screening chemicals for bioaccumulation potential, thereby prioritizing those likely to achieve elevated internal concentrations that could influence toxicity outcomes. Regulatory agencies such as the U.S. Environmental Protection Agency (EPA) and the European Chemicals Agency (ECHA) under REACH use BCF thresholds—typically above 1000 L/kg for initial concern or 2000 L/kg for bioaccumulative classification—to flag substances for targeted testing, particularly for endpoints like endocrine disruption where organismal dosimetry is essential.^[36]^[87] This approach allocates resources toward chemicals with heightened exposure risks in aquatic environments, informing decisions on whether to pursue advanced assays for chronic or sublethal effects.^[23] Quantitative structure-activity relationship (QSAR) models facilitate rapid BCF estimation for untested compounds by correlating molecular features, such as the octanol-water partition coefficient (K_{OW}), with accumulation behavior, enabling efficient prioritization without exhaustive experimentation. Validated against datasets from standardized fish exposure studies, these models—often linear regressions linking log BCF to log K_{OW}—support integration with toxicity screening tools, such as fish embryo acute toxicity tests (FET, OECD TG 236), to assess combined bioaccumulation and hazard potential for data-limited substances.^[88]^[89] For example, predicted high BCFs prompt evaluation of internal doses in embryo models to identify true risks rather than accumulation alone.^[90] Critically, elevated BCF forecasts highlight the necessity for dose-response characterization, as bioaccumulation does not equate to inherent toxicity; many substances concentrate in tissues without eliciting harm at environmentally relevant levels, averting regulatory overreach on non-toxic accumulators. This distinction ensures hazard assessments focus on verifiable adverse effects, distinguishing benign persistence from toxicological concern.^[91]^[92]

Body Burden Assessments

Body burden represents the total mass of a contaminant accumulated in an organism's tissues, frequently estimated via bioconcentration factors (BCFs) under steady-state conditions as BCF multiplied by the environmental medium concentration, adjusted for organism biomass to yield absolute load.^[93] For dynamic scenarios, kinetic models integrate uptake from water or diet and depuration processes, enabling predictions of time-dependent burdens critical for assessing post-exposure residues in mobile species like fish.^[94] In sportfish evaluations, body burdens of persistent contaminants such as polychlorinated biphenyls (PCBs) and mercury guide consumption advisories by linking organismal accumulation to potential human intake. Kinetic depuration modeling, incorporating first-order elimination rates, reveals PCB half-lives in fish tissues ranging from approximately 20 days for lower-chlorinated congeners to over 200 days for highly chlorinated ones, influencing residual levels after migration or seasonal exposure variations.^[95] Similarly, methylmercury depuration in predatory fish exhibits half-lives of 1-2 years, underscoring slow clearance that sustains elevated burdens in long-lived species.^[94] Empirical assessments of monitored U.S. sportfish demonstrate that body burdens of most contaminants yield low human health risks at moderate consumption rates, such as 1-2 servings (8-12 ounces) per week for general populations, with nutrient benefits like omega-3 fatty acids typically outweighing toxicological concerns except in high-exposure locales or sensitive subgroups.^[96] For instance, nationwide surveys indicate average mercury concentrations in freshwater fish below levels prompting unrestricted advisories for adults, affirming minimal non-cancer risks and negligible cancer increments from typical intakes.^[93] PCB burdens in coastal species similarly pose limited threats when depuration and dietary guidelines are factored, as evidenced by stable low incidence of related health endpoints in consumer cohorts.^[95]

Limitations and Debates

Model Uncertainties and Empirical Discrepancies

Bioconcentration factor (BCF) models frequently overpredict accumulation for compounds subject to biotransformation, as many frameworks underestimate metabolic elimination rates in target organisms such as fish. For instance, mechanistic models assuming negligible metabolism can exceed measured BCFs by factors of 10 to 100 for pharmaceuticals and other xenobiotics with hepatic or gill-based detoxification pathways, since empirical studies demonstrate that oxidative and conjugative processes reduce steady-state tissue concentrations.^[97] This discrepancy arises because in vitro-derived metabolic rate constants, when extrapolated to whole-organism scales, often fail to capture species-specific enzyme efficiencies or induction effects under chronic exposure.^[98] Ionization states introduce further predictive uncertainties, particularly for weak acids and bases where pH-dependent speciation alters membrane permeability and intracellular trapping. Models relying on neutral octanol-water partition coefficients (Kow) without corrections for ionized fractions can overestimate BCFs by ignoring reduced uptake across lipid bilayers or enhanced efflux via pH gradients, with deviations amplified in environmentally relevant pH ranges (6.5–8.5).^[99]^[100] Empirical validations, such as those for ionizable pharmaceuticals in aquatic invertebrates, show that liposome-water partitioning adjusted for pH better aligns predictions with observations, underscoring the need for speciation-inclusive parameters to mitigate overestimations.^[57] Comparative analyses reveal broader variances between modeled and measured BCFs due to unaccounted kinetic factors like growth dilution and variable gill ventilation rates. Arnot and Gobas (2006) reviewed over 5,000 BCF datasets, finding that while equilibrium partitioning models correlate with hydrophobicity (log Kow) for non-metabolites, residuals exceed an order of magnitude for 20–30% of cases, attributable to omitted physiological dynamics rather than analytical errors alone.^[4] Additionally, some empirical datasets exhibit inverse relationships between exposure concentration and normalized BCF, challenging the core steady-state assumption of concentration-independent partitioning and indicating potential saturation of uptake transporters or adaptive elimination not parameterized in standard simulations.^[89] These patterns emphasize that direct measurements remain essential for validation, as simulations alone propagate uncertainties from input parameters like lipid normalization or exposure duration.

Criticisms of Regulatory Overreliance

Regulatory frameworks often employ bioconcentration factor (BCF) thresholds, such as values exceeding 2000 L/kg, to identify substances as potentially bioaccumulative under persistent, bioaccumulative, and toxic (PBT) criteria, triggering restrictions even absent direct evidence of toxicity.^[101] Critics argue these fixed cutoffs may erroneously flag safe chemicals, as high BCF values do not inherently correlate with adverse effects, and empirical assessments reveal many flagged substances pose negligible risks when toxicity and exposure are considered.^[102] For instance, log Kow cutoffs—commonly set at 3 or 4 to proxy bioaccumulation potential—have been deemed overly conservative, with analyses indicating thresholds could safely rise to 4.5 across jurisdictions without compromising human or environmental protection, thereby avoiding undue classification of non-problematic compounds.^[70] ^[103] Exposure dependency further undermines reliance on static BCF thresholds, as demonstrated by studies on metals showing an inverse relationship between BCF and aqueous concentration: lower exposures yield higher BCFs, while realistic environmental levels often produce lower accumulation than lab-derived values suggest.^[104] McGeer et al. (2003) documented this pattern across multiple species and metals, highlighting how fixed criteria ignore concentration-driven uptake regulation, potentially leading regulators to overestimate hazards at typical field concentrations.^[105] Such dependencies imply that lab BCFs, normalized to low concentrations, inflate perceived risks, prompting policies that prioritize accumulation proxies over integrated exposure-toxicity assessments.^[106] This overreliance can stifle innovation by imposing preemptive bans or testing burdens on beneficial substances, such as pesticides or pharmaceuticals, where high modeled BCFs based on conservative log Kow assumptions deter development despite field evidence of limited real-world accumulation or harm.^[107] Data quality issues exacerbate this, with reviews finding up to 45% of reported BCFs plagued by uncertainties or measurement errors that systematically overestimate values, resulting in regulatory false positives.^[4] Empirical scrutiny favors contextual evaluations—incorporating metabolism, depuration rates, and site-specific exposures—over blanket thresholds, as unnuanced application equates mere partitioning with toxicity, diverting resources from genuine threats.

Distinctions from Bioaccumulation and Biomagnification

Bioconcentration refers to the uptake and retention of a substance solely from the surrounding aqueous medium, excluding dietary contributions, and is typically quantified under controlled conditions to isolate passive diffusion across gills or skin in aquatic organisms.^[2] Bioaccumulation, by contrast, integrates exposure from both water and contaminated prey, resulting in potentially higher or lower tissue residues depending on dietary assimilation efficiency and elimination rates, as captured by the bioaccumulation factor (BAF).^[109] Failure to distinguish these processes can lead to inflated predictions of organismal burdens in field settings, where food web dynamics often mitigate aqueous-driven concentrations through growth dilution or biotransformation.^[110] Biomagnification requires a trophic magnification factor (TMF) greater than 1, signifying net increase in chemical concentration per trophic level rise, driven by efficient dietary transfer exceeding losses from metabolism or egestion; bioconcentration alone does not necessitate this outcome, as initial aqueous uptake may diminish up the chain via biodilution (TMF <1).^[111] Empirical studies confirm this divergence: mercury often biomagnifies (TMF >1) in piscivorous fish, while copper, zinc, and lead typically biodilute due to homeostatic regulation and poor assimilation, even in systems with high bioconcentration at basal levels.^[112] Arsenic and nickel likewise exhibit trophic dilution across global marine webs, underscoring that hydrophobicity or persistence—key to bioconcentration—does not guarantee magnification without site-specific validation.^[113] Environmental reporting and advocacy frequently blur these concepts, portraying waterborne accumulation as a direct precursor to food chain escalation without TMF data, thereby overstating human and ecological risks for substances that degrade or excrete trophically rather than amplify.^[110] Such conflation ignores causal evidence that biomagnification hinges on low elimination kinetics and high lipid partitioning across multiple transfers, not merely initial bioconcentration, potentially diverting regulatory focus from verifiable threats.^[114]

References

[1]
[PDF] Technical Support Document Volume 2: Development of National ...
BIOCONCENTRATION. Bioconcentration. The net accumulation of a chemical by an aquatic organism as a result of uptake directly from the ambient water, through ...
[2]
EPA ExpoBox Terminology | US EPA
Jan 2, 2025 · Bioconcentration refers to direct transfers of the chemical from the surrounding environmental medium into the plant or animal (e.g., a ...
[3]
Comparison between bioconcentration factor (BCF) data ... - PubMed
The bioconcentration factor (BCF) is the ratio of the concentration of a chemical in an organism to the concentration in the surrounding environment at steady ...
[4]
A review of bioconcentration factor (BCF) and bioaccumulation ...
A review of 392 scientific literature and database sources includes 5317 bioconcentration factor (BCF) and 1656 bioaccumulation factor (BAF) values measured ...Missing: paper | Show results with:paper
[5]
Exposure Assessment Tools by Media - Aquatic Biota | US EPA
Apr 1, 2025 · Bioconcentration refers to direct transfers of the chemical from the surrounding environmental medium into the animal - it does not account for ...
[6]
Workgroup Report: Review of Fish Bioaccumulation Databases ...
In this article we review of the quantity, features, and public availability of bioconcentration, bioaccumulation, and biota–sediment accumulation data.Missing: paper | Show results with:paper
[7]
[PDF] Procedures for Derivation of Bioaccumulation Factors - NY.Gov
Mar 23, 1995 · Bioconcentration factor (BCF). The ratio (in L/kg) of a substance's concentration in tissue of an aquatic organism to its concentration in ...
[8]
[PDF] Ecological Effects Test Guidelines OCSPP 850.1730: Fish ...
Depuration phase is the portion of a bioconcentration test after the uptake phase, during which the organisms are in flowing water to which no test substance is ...
[9]
Dynamics of hydrophobic organic chemical bioconcentration in fish
Abstract -A model is presented describing the kinetics of uptake and release of nonmetabolizing organic chemicals by fish from water.
[10]
Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in ...
In contrast, the diffusion-layer-controlled process becomes dominant for. hydrophobic chemicals (log K. ow. > 3–4), meaning the opposite condition prevails.
[11]
Uptake, distribution and elimination of chemicals in fish
This work tries to give an overview on the most important physiological parameters for toxicokinetics in fish. Our approach is to analyse the major resistances ...
[12]
Review Cytochrome P450 (CYP) in fish - ScienceDirect.com
Studies of fish P450s have provided insights into the regulation of P450 genes by environmental stresses including water pollution.Missing: elimination bioconcentration
[13]
Assessing the reliability of uptake and elimination kinetics modelling ...
This study considers whether the current standard toxicokinetic methods are an accurate and applicable assessment of xenobiotic exposure in an aquatic ...
[14]
Toward improved models for predicting bioconcentration of well ...
Mar 15, 2013 · The purpose of this report is to provide updated models for predicting the bioconcentration of well-metabolized chemicals by rainbow trout. The ...
[15]
Ecological Effects Test Guidelines OCSPP 850.1730 - epa nepis
... uptake and elimination kinetics applies. Bioconcentration factor, steady-state (BCFss) refers to the BCF that exists when uptake and elimination are equal ...
[16]
[PDF] DDT: ITS EFFECT ON FISH AND WILDLIFE
Sixty-nine percent of all observed fish losses occurred within 34 hours after spraying. The warmer-water fishes-common shiners, common suckers, and golden ...
[17]
Fish and Other Wildlife as Sentinels - NCBI - NIH
Shortly after the introduction of organochlorine pesticides (e.g., DDT, heptachlor, endrin, aldrin, and dieldrin) in the 1940s and 1950s, dead birds were ...
[18]
Bureau of Sport Fisheries and Wildlife Pesticide-Wildlife Review: 1959
Major topics discussed are: Scope of Pesticide-Wildlife Problem; Effects on Wildlife-General; Laboratory Studies and Toxicology; Direct and Indirect Effects of ...Missing: 1950s | Show results with:1950s
[19]
[PDF] DDT and Other Organohalogen Pesticides in Aquatic Organisms
In the marine environment, fish surveys indicated that benthic feeders have higher concentra- tions of DDT than pelagic feeders (ATSDR 2002, NOAA and EPA 2007).
[20]
[PDF] DDT: A Review of Scientific and Economic Aspects of the Decision ...
Apr 11, 2001 · first-hand observed eflects of DDT oh fish and birdllfe, reporting lethal or tub'acute effects on aquatic and avian life exposed In. DDT ...
[21]
Review of Fish Bioaccumulation Databases Used to Identify ...
In this article we review of the quantity, features, and public availability of bioconcentration, bioaccumulation, and biota–sediment accumulation data.
[22]
Estimation of Bioconcentration in Marine Species with Structure ...
Neely et_ al^ (197&) suggested that the n-ocranol/water partition coefficient (P) be used as an estimator of the BCF. The measure- ment and estimation with (?) ...Missing: Kow | Show results with:Kow
[23]
[PDF] Test No. 305: Bioaccumulation in Fish: Aqueous and Dietary ... - OECD
Oct 2, 2012 · Test 305 is about bioaccumulation in fish via aqueous and dietary exposure, aiming to reduce complexity and use fewer fish.
[24]
Determination of lipid content in fish samples from bioaccumulation ...
Apr 3, 2012 · Bioconcentration factors for lipophilic compounds estimated on a wet weight basis (BCFW) increase with increasing lipid content. The generally ...
[25]
Relationship between the lipid content of fish and their ...
A significant positive correlation between the lipid content of eight fish species and their bioconcentration factor (BCF) of 1,2,4-trichlorobenzene is ...
[26]
Size-dependent bioaccumulation of metals in the amphipod ...
Aug 6, 2025 · It has been shown that bioconcentration factors for Cd, Cu, Pb and Zn decreased with increasing body length in the amphipod Gammarus zaddachi ( ...
[27]
A Modeling Case Study of Cyclic Volatile Methyl Siloxanes
We then explore the effect of variation in body mass for fish using the allometric relation in eq 6. For Daphnia, the above body size scaling is not valid and ...
[28]
Statistical relationship between metabolic decomposition and ... - NIH
Aug 7, 2018 · PSO reveals a relationship between chemical uptake and decomposition parameter values that predicts chemical-specific BCF values with moderate ...Fig. 1 · Fig. 3 · Fig. 4
[29]
[PDF] Annex VI Preliminary guidance paper on bioaccumulation evaluation
Metabolism (Biotransformation). The rate of metabolism rather than uptake determines the chemical's potential to bioaccumulate and biomagnify. High ...<|separator|>
[30]
Reproductive effects and bioconcentration of 4-nonylphenol in ...
The objective of this study was to investigate the reproductive success (fertility and fecundity) of medaka (Oryzias latipes) exposed to 4-nonylphenol ...
[31]
[PDF] A Generic QSAR for Assessing the Bioaccumulation Potential of ...
For example, the BCF- QSAR predicts chemicals with a log KOW range between approximately 4.5 and 8 to exhibit a BCF greater than 5 000. The regression model ...
[32]
[PDF] Document N° 41 - ECETOC
The Fi s are correction factors for specific chemical groups. The above QSAR shows that the bioconcentration potential (BCF) increases with the logKow up to a ...
[33]
Molecular size cutoff criteria for screening bioaccumulation potential ...
Feb 4, 2010 · The European Commission Technical Guidance Document states that substances with a molar mass (MW) >700 g/mol are less likely to be absorbed and ...
[34]
ASSESSING THE BIOACCUMULATION POTENTIAL OF IONIZABLE ...
For neutral organic chemicals, reported chemical uptake efficiencies in the gut (ED) tend to be fairly constant over a broad range of hydrophobicity (KOW), with ...Missing: Kow | Show results with:Kow
[35]
Novel Approach for Characterizing pH-Dependent Uptake of ...
May 29, 2017 · Here, we present and evaluate a combined experimental and modeling approach for characterizing the uptake of ionizable chemicals from water ...
[36]
[PDF] Fish Bioconcentration Data Requirement: Guidance for Selection of ...
Jul 15, 2020 · While OPP has not established a BCF value of regulatory concern per se, it has used a BCF of 1,000 as a point of departure for devoting.
[37]
Predicting the Bioconcentration Factor in Fish from Molecular ...
The bioconcentration factor (BCF) is one of the metrics used to evaluate the potential of a substance to bioaccumulate into aquatic organisms.Missing: salmon lean
[38]
Effects of temperature and salinity on bioconcentration and ...
Temperature and salinity had significant effects on biotransformation and BCFs. •. Implications for trophic transfer of contaminants in aquatic systems ...
[39]
Explicit Consideration of Temperature Improves Predictions of ...
Oct 25, 2022 · This study implemented the effect of temperature in a widely used modeling framework, the General Unified Threshold model for Survival (GUTS).
[40]
Speed it up: How temperature drives toxicokinetics of organic ...
3.6. Temperature and species dependence of the bioconcentration factor. For most compounds, the BCFkin remained stable across different temperatures (slope not ...
[41]
Effect of pH on the accumulation kinetics of pentachlorophenol in ...
The pH-related changes in the pharmacokinetics of PCP resulted in a decrease in its bioconcentration factor with an increase in pH and account both for the ...
[42]
Effect of pH on the toxicity and bioconcentration of sulfadiazine on ...
Furthermore, the bioconcentration factor (dry weight) was determined to be 50 and 36 at pH 6.0 and 8.5, respectively. The higher toxicity at the lower pHs was ...
[43]
Observed and modeled effects of pH on bioconcentration of ...
Apr 28, 2015 · A need exists to better understand the influence of pH on the uptake and accumulation of ionizable pharmaceuticals in fish.
[44]
Bioconcentration of perfluorinated compounds in blackrock fish ...
Jul 24, 2010 · A significant correlation was found between bioconcentration factors (BCF) of PFCs and salinity, except for PFOA, possibly resulting from ...
[45]
Evaluation of Published Bioconcentration Factor (BCF) and ...
Feb 19, 2021 · The assembled data were evaluated for quality, and for gaps and limitations in bioaccumulation information for the PFAS universe of chemicals.Missing: key | Show results with:key
[46]
https://pubmed.ncbi.nlm.nih.gov/9734324
[47]
Effects of water hardness and dissolved organic material on ...
Nov 2, 2009 · The bioconcentration of pyrene and TCB increased with increasing water hardness in the presence of KL DOM. In conclusion, the effects of DOM and ...
[48]
Sorption of Highly Hydrophobic Organic Chemicals to Organic ...
Jun 30, 2016 · Especially OM in the test water can have a high impact on the bioavailability of hydrophobic organic chemicals (HOCs) in bioconcentration ...
[49]
Test No. 305: Bioaccumulation in Fish: Aqueous and Dietary Exposure
BCF and BMF are expressed based on the total concentration in fish, i.e. per total wet weight of the fish, and as normalized to a fish with a 5% lipid content.
[50]
Test No. 305: Bioconcentration: Flow-through Fish Test | OECD
During the test, dissolved oxygen, TOC, pH, total hardness and salinity, and temperature should be measured in vessels. The lipid content should be determined ...Missing: variables | Show results with:variables<|control11|><|separator|>
[51]
Reducing the number of fish in bioconcentration studies with ...
The OECD flow-through fish test (test guideline [TG] 305) requires the use of at least three experimental groups (a control plus a low and high concentration ...Missing: biological variables
[52]
[PDF] ECO33: Use & Interpretation Of Dietary Bioaccumulation Tests For ...
Apply & test methods for deriving bioaccumulation metrics (including the BCF) from the results of OECD 305 dietary bioaccumulation tests. • Develop methods for ...
[53]
[PDF] Bioaccumulation and Aquatic System Simulator (BASS) User's ... - EPA
where ku, ke, and km are the fish's uptake rate, elimination rate, and ... bioconcentration factor Kf via Eq. (2-7) appears to require a great deal of ...
[54]
Growth‐Correcting the Bioconcentration Factor and ... - NIH
Second, we applied the framework to assess the BCF and BMF of a chemical in nongrowing fish and compared the results to those derived from using the OECD‐305‐ ...
[55]
Measuring and Estimating the Bioconcentration Factor of Chemicals ...
... equation log BCF = 0.85 log P − 0.70, which permits the estimation of the bioconcentration factor of chemicals to within 60% before laboratory testing. The ...
[56]
Assessment of an approach to estimating aquatic bioconcentration ...
The relationship between KOW and the BCF developed by Veith et al. [17] is. 2 log(BCF ) ⫽ 0.85·log(K ) ⫺ 0.7, r ⫽ 0.95. (11) pred. OW where BCFpred is the ...
[57]
Predicting the bioconcentration factor of highly hydrophobic organic ...
Arnot and Gobas, 2006. J.A. Arnot, F.A.P.C. Gobas. A review of bioconcentration factor (BCF) and bioaccumulation factor (BAF) assessments for organic ...
[58]
How effective is score-based data quality assessment? An ...
Dec 1, 2024 · Overall, the fish BCF dataset contains a significant fraction of high-quality data, with nearly 60% of all reported BCF (n = 4367) evaluated as ...
[59]
Models Used to Predict Chemical Bioaccumulation in Fish ... - NIH
The K FW may be estimated by any one of several well-known equations that relate log BCF to log K OW (Oliver & Niimi, 1983; Veith et al., 1979).
[60]
Fugacity-Based Model of PCB Bioaccumulation in Complex Aquatic ...
A fugacity-based model is developed to simulate the phenomena of bioconcentration and biomagnification of organic contaminants in complex food webs in ...Missing: 1982 | Show results with:1982
[61]
Finding fugacity feasible, fruitful, and fun - MacKay - SETAC Journals
Nov 5, 2009 · The fugacity approach enables the multimedia character of organic chemicals to be deduced, thus contributing to the evaluation of chemical ...
[62]
A model for predicting the bioaccumulation of hydrophobic organic ...
Gobas, D. Dolan. Virtual elimination of toxic and persistent chemicals from the Great Lakes: the role of mass balance models. International Joint Commission ...
[63]
Mechanistic modeling of the bioconcentration of (super)hydrophobic ...
Feb 15, 2023 · We here present the first mechanistic model for H. azteca that considers the single diffusive processes in the gills and gut.Missing: Kow | Show results with:Kow
[64]
Bioconcentration of Several Series of Cationic Surfactants in ...
Jun 16, 2021 · (33) The flow of water across the gills and the flow of blood through the gills were modeled as perfusion limited resistances that constrain ...
[65]
Development and evaluation of a mechanistic bioconcentration ...
Sep 28, 2012 · The key task is then to integrate chemical partitioning and distribution information with uptake and elimination kinetics. Advantages of ...
[66]
Predictive modeling and interpretability analysis of bioconcentration ...
Jul 15, 2025 · In this study, a machine learning (ML) model was developed to predict BCF values using molecular descriptors and 9 algorithms.
[67]
Machine learning-based q-RASAR predictions of the ... - PubMed
Nov 5, 2024 · In this study, we utilized an innovative quantitative read-across (RA) structure-activity relationship (q-RASAR) approach to predict the bioconcentration ...
[68]
The (Re)-Evolution of Quantitative Structure–Activity Relationship ...
Nov 28, 2022 · The multi-instance QSAR model outperformed the single-instance ones in most cases while alleviating the difficulty of selecting the bioactive ...
[69]
[annexXIII] REACH - Registration, Evaluation, Authorisation and ...
A substance fulfils the bioaccumulation criterion (B-) when: - the bioconcentration factor (BCF) is higher than 2000. The assessment of bioaccumulation shall be ...Missing: threshold | Show results with:threshold
[70]
Are current regulatory log Kow cut-off values fit-for-purpose as a ...
In this study, the experimentally determined log Kow and fish bioconcentration factors (BCF) of 532 chemicals were compared. While the analysis underlined ...
[71]
Screening for low aquatic bioaccumulation with QSAR ...
For substances produced or imported between 10 and 100 tonnes per year, BCF studies are not required but BCFs are needed for PBT (persistent, bioaccumulative, ...
[72]
[PDF] Methodology for Deriving Ambient Water Quality Criteria for ... - EPA
BCF. Bioconcentration factor based on total concentrations in tissue and water. BEF. Bioaccumulation equivalency factor. BMF. Biomagnification factor. BSAF.
[73]
[PDF] guideline-environmental-risk-assessment-medicinal-products ...
Aug 22, 2024 · Bioconcentration factor (BCF). The BCF is determined in fish using the OECD 305 test guideline. Aqueous exposure is the preferred methodology ...
[74]
[PDF] Environmental Risk Assessment of Difficult Substances - ECETOC
The PNEC is derived from ecotoxicity data by application of appropriate safety factors ... the concentration of the substance in water. Bioconcentration Factor ( ...
[75]
[PDF] Derivation of PFAS Ecological Screening Values - DOD DENIX
These ESVs are solely for use in conducting screening-level ecological risk assessments and are not recommended or intended for use as default cleanup values ( ...
[76]
Bioaccumulation of per- and polyfluoroalkyl substances by ...
Mar 25, 2023 · Increasing organic carbon decreased PFAS bioaccumulation to benthic invertebrates. PFAS bioaccumulation in worms was much higher than in mussels and snails.
[77]
Evaluation of Published Bioconcentration Factor (BCF) and ...
BAFs for some PFASs appear consistent with BCFs developed with laboratory experiments, in which values decline with increasing concentrations in water. This ...
[78]
[PDF] A review of bioconcentration factor (BCF) and bioaccumulation ...
The degree to which bioconcentration occurs is expressed as the bioconcentration factor (BCF) and can only be measured under controlled laboratory conditions in ...
[79]
[PDF] Using a probabilistic approach in an ecological risk assessment ...
A probabilistic approach was applied in an ecological risk assessment (ERA) to characterize risk and address uncer- tainty employing Monte Carlo simulations ...
[80]
Mathematical relationships between metrics of chemical ... - PubMed
The BCFK s and the values of M can be regarded as the fundamental determinants of bioaccumulation and biomagnification in aquatic food webs. Analyzing data from ...
[81]
EPA EcoBox Tools by Exposure Pathways - Food Chains
May 21, 2025 · Compounds can transfer from an environmental medium to biota, a process referred to as bioconcentration or bioaccumulation. Bioaccumulation ...
[82]
Normalizing the Biomagnification Factor - Gobas - SETAC Journals
Dec 8, 2020 · We illustrate with theoretical models and experimental data that the BMF of lipophilic chemicals is a function of the lipid content of the diet.
[83]
Development and Evaluation of Aquatic and Terrestrial Food Web ...
To assess model performance, we compared model predicted bioaccumulation metrics of PFAS (e.g., BCF, BAF, BMF) or predicted concentrations of PFAS in organism ( ...Missing: adjustments | Show results with:adjustments
[84]
Preferential biomagnification of aryl hydrocarbon hydroxylase ...
The results indicate that both total PCB and AHH-inducing PCBs biomagnify with increasing food web trophic level, with biomagnification of the AHH-inducing PCBs ...Missing: empirical data
[85]
Model of PCB in Lake Michigan lake trout food chain - ResearchGate
Aug 6, 2025 · The model indicates that for the top predator lake trout, PCB exposure through the food chain can account for greater than 99% of the observed ...
[86]
A Bayesian assessment of polychlorinated biphenyl contamination ...
The present study attempts a rigorous assessment of the spatio-temporal PCB trends in multiple species across the Canadian waters of the Great Lakes.
[87]
How error‐prone bioaccumulation experiments affect the risk ...
Nov 21, 2022 · ... bioaccumulation potential have thresholds for the BCF. Under REACH in the EU, a BCF > 2000 indicates a bioaccumulative substance, and a BCF ...
[88]
Assessment and validation of the CAESAR predictive model for ...
Bioconcentration factor (BCF) describes the behaviour of a chemical in terms of its likelihood of concentrating in organisms in the environment.Missing: definition | Show results with:definition<|separator|>
[89]
Variability in fish bioconcentration factors: Influences of study design ...
The BCF values were lipid-normalized as advised in the OECD TG 305 and were log-transformed as standard deviation (SD) was correlated with BCF values (Fig.
[90]
Impact of Biotransformation on Internal Concentrations and ...
Sep 24, 2024 · Development of a General Baseline Toxicity QSAR Model for the Fish Embryo Acute Toxicity Test. Chemosphere 2016, 164, 164– 173, DOI: 10.1016 ...
[91]
Inverse relationship between bioconcentration factor and exposure ...
Nov 3, 2009 · This study illustrates that the BCF/BAF criteria, as currently applied, are inappropriate for the hazard identification and classification of metals.
[92]
Bioconcentration, bioaccumulation, biomagnification and trophic ...
Dec 8, 2017 · Studies of bioaccumulation fall generally into one of the following categories: ecosystem monitoring, laboratory tests under controlled ...Missing: history | Show results with:history
[93]
[PDF] Guidance for Assessing Chemical Contaminant Data for Use in Fish ...
Use and occurrence. Pharmacokinetics. Target tissues. Absorption. Deposition-bioaccumulation potential/half-life/body burden. Metabolism. Excretion. Susceptible ...
[94]
Modeling the Elimination of Mercury by Fish - ACS Publications
The importance of dynamic mercury water column concentrations on body burdens in a planktivorous fish: A bioenergetic and mercury mass balance perspective.
[95]
Toxicology, Kinetics and Metabolism of PCBs in Fishes, With Special ...
Thus, as body burdens of PCBs in fish change due to selective elimination of lower chlorinated congeners, one might expect potential toxicity to increase.
[96]
Public health benefits and risks of fish consumption - NIH
Substantial evidence suggests that the incorporation of fish in the diet reduces mortality from CHD( , ), the leading cause of death in developed and several ...Missing: sportfish | Show results with:sportfish
[97]
Impact of Biotransformation on Internal Concentrations and ... - NIH
This would lead to underestimation of specific toxicity, which could lead to a wrong classification of chemicals. Ideally, measured ICs in combination with ...
[98]
The influence of gill and liver metabolism on the predicted ...
Aug 10, 2025 · Recent efforts to refine BCF models have focused on physiological factors, including the ability of the organism to eliminate the compound ...
[99]
Predicting the Accumulation of Ionizable Pharmaceuticals and ...
Aug 3, 2022 · Ion trapping effects may therefore add considerable uncertainty to the prediction of translocation and accumulation (Briggs et al., 1987 ...
[100]
Predicting the Accumulation of Ionizable Pharmaceuticals and ... - NIH
(2012) observed that the pH-corrected liposome–water partition coefficient was a good predictor of bioconcentration factors (BCFs) for aquatic invertebrates (R2 ...
[101]
Intelligent consensus predictions of bioconcentration factor of ...
Introduction. The bioconcentration factor (BCF) plays a crucial role in the risk assessment of chemical contaminants from the ecological point of view. The BCF ...
[102]
How error‐prone bioaccumulation experiments affect the risk ...
We have demonstrated in the present study that the experimental BCF values based on aqueous exposure very often underestimate the bioaccumulation potential for ...
[103]
Are current regulatory log Kow cut-off values fit-for-purpose as a ...
Dec 28, 2023 · Our analysis shows that the log Kow cut-off could be increased to 4.5 in all regions for all purposes without leading to a reduced protection ...Missing: debate | Show results with:debate
[104]
implications for hazard assessment of metals in the aquatic ...
The bioconcentration factor (BCF) and bioaccumulation factor (BAF) are used as the criteria for bioaccumulation in the context of identifying and ...
[105]
Inverse Relationship Between Bioconcentration Factor and ...
Aug 6, 2025 · Mean BCF/BAF values for Hg were also variable, but the inverse relationship was equivocal, in contrast with HCB, which conformed to the BCF ...
[106]
[PDF] inverse relationship between bioconcentration factor and exposure
This work examines the theoretical and experimental basis for the use of BCF/BAF in the hazard assessment of. Zn, Cd, Cu, Pb, Ni, and Ag. As well, BCF/BAFs ...
[107]
Evaluation of the EMA log kow trigger for fish BCF testing based on ...
We report experimental log Kow and BCF values for 64 human pharmaceuticals that were used to evaluate the current BCF testing trigger of log Kow ≥3.
[108]
A review of bioconcentration factor (BCF) and bioaccumulation ...
Aug 9, 2025 · A review of 392 scientific literature and database sources includes 5317 bioconcentration factor (BCF) and 1656 bioaccumulation factor (BAF) values measured ...Missing: facts | Show results with:facts
[109]
[PDF] Appendix A. Glossary of Useful Terms - EPA
Bioconcentration – The accumulation of a chemical in fatty tissues (lipids) of a fish or other organism to levels greater than that in the surrounding medium.
[110]
Processes influencing chemical biomagnification and trophic ...
Measurements of TMFs in specific ecosystems can provide invaluable confirmation that biomagnification or biodilution has occurred across food webs, but ...
[111]
Trophic magnification factors: Considerations of ecology ...
For TMF > 1 (b > 0), the chemical biomagnifies through the food web, with an average factor of TMF per TL. For TMF < 1 (b < 0), the chemical decreases, with an ...
[112]
Quantifying the bioaccumulation and trophic transfer processes of ...
Jun 10, 2024 · In summary, Hg exhibited a significant biomagnification, whereas Cu, Zn, Pb experienced biodilution. The fish dietary health risk assessment for ...
[113]
Evaluation on the biomagnification or biodilution of trace metals in ...
The results showed dissimilar trophic transfer behaviors of these metals in global marine food webs, in which As and Ni tended to be efficiently biodiluted ...Missing: empirical | Show results with:empirical
[114]
Use of trophic magnification factors and related measures to ...
Apr 27, 2011 · For chemicals with TMFs that are statistically greater than 1, the absolute value of the TMF is not usually relevant in the bioaccumulative ...