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Protein S

Protein S is a vitamin K-dependent plasma glycoprotein that functions as a nonenzymatic cofactor to activated protein C (APC) in the proteolytic inactivation of coagulation factors Va and VIIIa, thereby regulating thrombin generation and preventing excessive blood clotting.^[1] Encoded by the PROS1 gene on chromosome 3, it is primarily synthesized in the liver as a single-chain molecule with a molecular weight of approximately 69-75 kDa, and it circulates in human plasma at concentrations of about 25 μg/mL, with roughly 40% in a free form and 60% bound to the complement regulator C4b-binding protein (C4BP).^[2] Deficiency in protein S, whether hereditary or acquired, significantly elevates the risk of venous thromboembolism, including deep vein thrombosis and pulmonary embolism, highlighting its critical role in hemostatic balance.^[3] Structurally, protein S comprises multiple modular domains that underpin its multifunctional properties: an N-terminal γ-carboxyglutamic acid (Gla) domain for calcium- and phospholipid-dependent membrane binding, followed by a thrombin-sensitive region (TSR), four epidermal growth factor-like (EGF-like) domains, and a C-terminal sex hormone-binding globulin (SHBG)-like domain responsible for interactions with APC and other partners.^[4] The Gla domain undergoes vitamin K-dependent carboxylation, enabling protein S to localize to negatively charged phospholipid surfaces on activated platelets and endothelial cells, where it enhances APC's serine protease activity against procoagulant substrates.^[1] Beyond its primary anticoagulant role, protein S also serves as a cofactor for tissue factor pathway inhibitor (TFPI) to inhibit the factor Xase complex and exhibits independent anti-inflammatory, profibrinolytic, and anti-apoptotic effects, including regulation of vascular permeability through pathways involving p38 MAPK and Rho/ROCK signaling.^[3]^[2] In clinical contexts, protein S levels are influenced by factors such as age, pregnancy, oral contraceptives, and conditions like liver disease, nephrotic syndrome, or disseminated intravascular coagulation, which can lead to acquired deficiencies mimicking hereditary forms.^[3] Hereditary deficiencies, often due to mutations in PROS1 resulting in type I (quantitative reduction), type II (qualitative dysfunction), or type III (isolated free protein S reduction) variants, follow an autosomal dominant inheritance pattern and affect 0.03-0.13% of the general population, with higher prevalence (up to 3-5%) among individuals with unexplained thrombophilia.^[3] Diagnosis typically involves measuring total and free protein S antigen and activity levels, with testing recommended after acute thrombotic events have resolved to avoid confounding influences.^[1]

Discovery and Nomenclature

Historical Discovery

Protein S was first identified in 1977 by Richard G. DiScipio and colleagues in Earl W. Davie's laboratory at the University of Washington in Seattle, during efforts to purify vitamin K-dependent proteins from bovine plasma. The protein was noted as an unknown component that consistently co-purified with protein C, another vitamin K-dependent factor, and was arbitrarily designated protein S in reference to its isolation site in Seattle. Initial biochemical characterization revealed it as a single-chain glycoprotein containing γ-carboxyglutamic acid residues, distinguishing it as part of the vitamin K-dependent family but without serine protease activity.^[5] In the early 1980s, further studies established protein S as a distinct entity from other coagulation factors, with human plasma isolations confirming similar properties to the bovine form.^[6] Its identity was solidified in 1986 through cDNA cloning efforts; the bovine sequence, determined by Dahlbäck et al., encoded a 635-amino-acid precursor with a prepro leader peptide, while the human cDNA, isolated by Lundwall et al., showed 82% homology to the bovine version and revealed structural similarities to other vitamin K-dependent coagulation factors such as factors IX and X, including Gla, epidermal growth factor-like, and laminin G-type domains. These cloning studies provided the genetic basis for understanding protein S as a non-enzymatic regulator in hemostasis.^[7]^[8] In the mid-1980s, family-based pedigree analyses linked heterozygous protein S deficiency to hereditary thrombophilia, marking a key milestone in recognizing its clinical relevance. Seminal studies, such as those by Comp et al. in 1984 and Schwarz et al. in 1984, documented recurrent venous thrombosis in affected kindreds, establishing an autosomal dominant inheritance pattern with incomplete penetrance and prompting classifications of deficiency types based on antigen and activity levels.^[9]^[10] These investigations, building on earlier case reports, underscored protein S's role in anticoagulant pathways through genetic segregation analyses in multi-generational cohorts. Subsequent studies in the 1990s, including those by Girolami et al. and Clark et al., further refined the understanding of deficiency variants.

Classification and Naming

Protein S is classified as a vitamin K-dependent plasma glycoprotein that serves as a non-inhibitory cofactor in the coagulation cascade, primarily enhancing the activity of activated protein C (APC) to inactivate procoagulant factors Va and VIIIa.^[1]^[11] Unlike serine protease inhibitors (SERPINs), Protein S does not directly inhibit proteases but modulates hemostasis through cofactor interactions, distinguishing it within the broader family of anticoagulant proteins.^[12] The nomenclature "Protein S" originates from its discovery in Seattle, Washington, in 1977, where researchers arbitrarily assigned the letter "S" to denote the site of isolation.^[3] The official gene symbol is PROS1, encoding Protein S alpha (the functional isoform), while a pseudogene PROS2 exists without transcriptional activity.^[13] Evolutionarily, Protein S exhibits homology to other vitamin K-dependent proteins, including Protein C, Factor IX, Factor X, Factor VII, and Protein Z, sharing a conserved Gla domain essential for calcium-mediated membrane binding in coagulation.^[14] This places Protein S phylogenetically within the subgroup of vitamin K-dependent cofactors derived from ancient gene duplications in early vertebrates, likely from a Factor VII/Factor X ancestor, though it lacks the serine protease catalytic domain found in its homologs.^[14] Protein S should not be confused with sex hormone-binding globulin (SHBG), a distinct plasma protein involved in steroid transport, despite structural similarity in Protein S's C-terminal SHBG-like domain, which consists of laminin G-type repeats and contributes to its non-anticoagulant functions.^[15] This homology underscores broader evolutionary links among extracellular binding proteins but highlights Protein S's unique role in hemostasis.^[16]

Molecular Biology

Gene Structure and Genetics

The PROS1 gene, which encodes protein S, is located on the long arm of chromosome 3 at the cytogenetic band 3q11.2.^[17] This gene spans approximately 80 kilobases of genomic DNA and consists of 15 exons interrupted by 14 introns.^[17] The exon-intron organization reflects the modular structure of the protein, with exons encoding distinct domains such as the Gla domain, thrombin-sensitive regions, and C-terminal segments.^[18] Hereditary deficiencies in protein S are inherited in an autosomal dominant manner, with heterozygous carriers at increased risk for thrombotic events.^[3] These deficiencies are classified into three main types based on the nature of the genetic and functional defects. Type I deficiency represents a quantitative defect characterized by reduced levels of both total and free protein S antigen due to null alleles, such as large deletions, nonsense mutations, or frameshifts that lead to absent or truncated protein production.^[3] Type II deficiency is a qualitative defect involving dysfunctional protein S variants that impair activity despite normal or near-normal antigen levels, often resulting from missense mutations affecting key functional domains.^[3] Type III deficiency features normal total protein S levels but reduced free protein S and activity, typically linked to variants that increase binding to C4b-binding protein or alter secretion dynamics.^[3] Over 450 mutations in PROS1 have been reported as of 2021, primarily in the Human Gene Mutation Database (HGMD), encompassing nonsense, missense, splice-site, and large structural variants associated with protein S deficiency.^[19]^[20] Notable examples include the nonsense mutation p.Arg451*, which introduces a premature stop codon in exon 10, leading to type I deficiency and increased venous thromboembolism risk in affected individuals.^[21] Missense mutations, such as those disrupting cysteine residues in the sex hormone-binding globulin-like domain, can cause type II deficiency by impairing protein stability or cofactor function.^[22] Homozygous or compound heterozygous variants are rare and often result in severe neonatal purpura fulminans.^[3] The prevalence of heterozygous PROS1 variants causing protein S deficiency is estimated at 0.03% to 0.13% in the general population, with higher rates observed in cohorts with venous thromboembolism (up to 1-2%). Recent population-scale studies as of 2025 report high-risk (frameshift and splice site) PROS1 variants at 0.0091% in the UK and 0.0178% in the US, and indicate that low protein S levels confer increased VTE risk even without coding variants in PROS1.^[23]^[24] These carriers exhibit a 2- to 11-fold increased odds ratio for venous thromboembolism compared to non-carriers, depending on the specific variant and environmental factors.^[25]^[26] Genetic testing for PROS1 variants typically involves Sanger sequencing to detect point mutations, small insertions/deletions, and splice-site changes across all exons.^[27] For large deletions or duplications, which account for up to 10-20% of cases, multiplex ligation-dependent probe amplification (MLPA) is employed to assess copy number variations.^[28] Next-generation sequencing panels may integrate both approaches for comprehensive analysis in thrombophilia evaluation.^[27]

Biosynthesis and Regulation

Protein S is primarily synthesized in hepatocytes, which serve as the main source of circulating protein S in plasma. Extrahepatic synthesis occurs in endothelial cells, megakaryocytes, and Leydig cells of the testes, contributing to local regulation of coagulation and other functions. In megakaryocytes, protein S is produced and stored in platelet α-granules, from where it can be released upon activation. This distributed synthesis allows protein S to participate in both systemic and tissue-specific processes.^[29]^[30]^[31] The PROS1 gene, located on chromosome 3, is transcribed into mRNA that undergoes processing, including alternative splicing to generate multiple transcript variants encoding the protein. Translation produces a 676-amino-acid pre-pro-protein, which is subsequently cleaved to yield the mature 635-amino-acid form. Transcriptional regulation of PROS1 is influenced by hormonal and environmental factors; estrogens downregulate expression through estrogen receptor α (ERα) interacting with Sp1 to recruit repressive complexes like RIP140 and HDAC3. Inflammatory cytokines such as TNF-α and IL-1α suppress protein S synthesis in hepatocytes and endothelial cells, often overriding any potential upregulation by IL-6, thereby reducing levels during acute inflammation. Hypoxia also downregulates expression via hypoxia-inducible factor 1α (HIF1α). As a vitamin K-dependent protein, its functional maturation relies on the vitamin K cycle mediated by VKORC1, though this primarily affects post-translational steps rather than transcription.^[29]^[32]^[33]^[34] Following translation, protein S enters the secretory pathway in the endoplasmic reticulum, where it undergoes essential post-translational modifications for functionality and stability. Gamma-carboxylation of 11 glutamic acid residues in the N-terminal Gla domain is catalyzed by gamma-glutamyl carboxylase in a vitamin K-dependent manner, enabling calcium-mediated binding to phospholipid surfaces. Additionally, beta-hydroxylation occurs at one aspartic acid in the first EGF-like domain and one asparagine in each of the other three EGF-like domains, contributing to structural integrity. The fully modified protein is secreted into circulation, where free protein S has a plasma half-life of approximately 42 hours. These processes ensure that only properly modified protein S is available for anticoagulant and other physiological roles.^[29]^[35]

Biochemistry

Protein Structure

Protein S is a vitamin K-dependent glycoprotein with a mature polypeptide chain consisting of 635 amino acids and an approximate molecular mass of 75 kDa.^[36] The protein exhibits a modular domain architecture typical of anticoagulant factors, comprising an N-terminal γ-carboxyglutamic acid (Gla) domain spanning residues 1–45 that enables calcium-dependent phospholipid binding, a thrombin-sensitive region (TSR) spanning residues 46–75, followed by four epidermal growth factor-like (EGF-like) domains encompassing residues 76–225, and a C-terminal sex hormone-binding globulin (SHBG)-like region covering residues 226–635.^[37] The Gla domain, which undergoes post-translational γ-carboxylation of specific glutamic acid residues to coordinate calcium ions, adopts a compact conformation essential for membrane association.^[38] The SHBG-like domain of Protein S forms a tandem repeat of two laminin G-type (LG) modules, LG1 and LG2, adopting the characteristic β-sandwich fold observed in sex hormone-binding globulin, with LG1 (approximately residues 226–358) and LG2 (residues 359–597) connected by a flexible linker.^[39] This region includes a unique C-terminal extension (residues 621–635) that contributes to phospholipid surface interactions and distinguishes Protein S from steroid-binding homologs like SHBG.^[40] The EGF-like domains, each approximately 40–50 residues long, feature conserved disulfide bonds and, in the case of EGF1, a β-hydroxylated aspartic acid residue that stabilizes the linker between domains. In its free form, Protein S circulates as a monomer, with about 40% of plasma Protein S unbound and active as an anticoagulant cofactor.^[11] Upon binding to C4b-binding protein (C4BP), it forms a non-covalent 1:1 heterocomplex primarily through interaction of the SHBG-like domain with the β-chain of C4BP, which sequesters approximately 60% of Protein S and attenuates its cofactor activity for activated protein C.^[41] Structural insights into the Gla domain conformation have been derived from NMR and homology modeling, revealing a dynamic, calcium-stabilized structure, while partial crystal structures of the LG domains highlight the conserved fold shared with laminin and SHBG.^[42]

Post-Translational Modifications

Protein S is subject to several key post-translational modifications that are critical for its structural integrity, calcium binding, and functional roles in anticoagulation. The most prominent is γ-carboxylation in the N-terminal Gla domain, where 11 glutamic acid residues (at positions 6, 7, 14, 16, 19, 20, 25, 26, 29, 32, and 36 of the mature protein) are converted to γ-carboxyglutamic acid (Gla) by the vitamin K-dependent γ-glutamyl carboxylase (GGCX). This process requires reduced vitamin K as a cofactor and occurs in the endoplasmic reticulum, enabling the chelation of multiple calcium ions that mediate the Ca²⁺-dependent binding of Protein S to negatively charged phospholipid membranes on activated platelets and endothelial cells, thereby positioning it for interactions with protein C and other cofactors.^[43]^[44] In addition to γ-carboxylation, Protein S undergoes β-hydroxylation on specific residues within its four epidermal growth factor-like (EGF-like) domains, catalyzed by the iron- and 2-oxoglutarate-dependent enzyme aspartyl/asparaginyl β-hydroxylase (AspH, also known as HAAH). This modification affects one aspartic acid residue in EGF1 and one asparagine residue each in EGF2, EGF3, and EGF4, resulting in β-hydroxyaspartic acid and β-hydroxyasparagine, respectively. These hydroxylated residues enhance calcium coordination within the EGF domains, contributing to the overall conformational stability and high-affinity calcium binding required for Protein S's modular architecture and interactions with binding partners.^[43]^[45]^[46] Glycosylation further modifies Protein S, accounting for a significant portion of its mature molecular mass of approximately 75 kDa (compared to the unmodified polypeptide of ~69 kDa). It features three major N-linked glycosylation sites at asparagine residues (Asn152 in the thrombin-sensitive region-proximal area, Asn217 in EGF2, and Asn458 in the sex hormone-binding globulin-like domain), as well as several O-linked sites primarily in the C-terminal region. These carbohydrate attachments, added in the endoplasmic reticulum and Golgi apparatus, promote proper protein folding, secretion, and circulatory half-life while preventing premature proteolysis, though they do not directly influence anticoagulant activity.^[43] Proteolytic processing occurs primarily in the thrombin-sensitive region (TSR), a 29-residue loop between the Gla domain and first EGF domain, where thrombin cleaves at specific arginine residues, including Arg52 and Arg70 (with an initial preference for Arg70 followed by Arg52). This cleavage generates a nicked form of Protein S, which, under limited proteolysis conditions, can exhibit altered cofactor properties for activated protein C, though extensive cleavage typically reduces its membrane-binding efficiency and anticoagulant function. The TSR's susceptibility to thrombin reflects a regulatory mechanism during coagulation activation.^[47]85250-3/fulltext) Structural stability of Protein S is maintained by 18 cysteine residues distributed across its EGF-like and sex hormone-binding globulin domains, forming nine intramolecular disulfide bonds that rigidify the multi-domain scaffold and protect against unfolding or aggregation in plasma. These bonds are conserved in evolution and essential for maintaining the protein's extended conformation, which facilitates simultaneous interactions with multiple partners like activated protein C and tissue factor pathway inhibitor.

Physiological Functions

Anticoagulant Activity

Protein S serves as a critical non-enzymatic cofactor for activated protein C (APC) in the protein C anticoagulant pathway, significantly enhancing the proteolytic inactivation of coagulation factors Va (FVa) and VIIIa (FVIIIa) on phospholipid membranes.^[48] This enhancement is particularly pronounced for FVa, where free Protein S increases the rate of APC-mediated cleavage at Arg306 by 20- to 30-fold, while providing a more modest 1- to 5-fold boost at Arg506; for FVIIIa, the effect is approximately 1.5-fold alone but up to 11-fold in the presence of factor V.^[48] These actions downregulate thrombin generation and prevent excessive clot formation during hemostasis.^[49] Additionally, Protein S functions as a cofactor for tissue factor pathway inhibitor (TFPI), enhancing TFPI's inhibition of the factor Xase complex (factor VIIIa-IXa) and thereby providing another layer of anticoagulant protection against excessive thrombin generation.^[50] The mechanism of Protein S's cofactor activity relies on its interaction with APC and membrane surfaces. Free Protein S binds to APC primarily through its epidermal growth factor-like (EGF) domains, particularly EGF1 and EGF2, which facilitate allosteric exposure of APC's active sites for substrate recognition and cleavage.^[48] Additionally, the γ-carboxyglutamic acid (Gla) domain of Protein S, containing 11 Gla residues, enables calcium-dependent binding to negatively charged phospholipids, localizing the Protein S-APC complex to the procoagulant membrane where FVa and FVIIIa reside.^[48] Only the free form of Protein S exhibits this APC cofactor function, as the bound form lacks these interactions.^[51] In human plasma, Protein S circulates at total concentrations of approximately 20-25 mg/L (300-350 nmol/L), with about 40% existing in the active free form and 60% bound to C4b-binding protein (C4BP), which renders it inactive as an APC cofactor.^[48]^[52] Deficiency in free Protein S, whether inherited or acquired, impairs the efficiency of the APC pathway, resulting in reduced inactivation of FVa and FVIIIa and a predisposition to hypercoagulability and thrombosis.^[49]^[53]

Non-Hemostatic Roles

Beyond its established role in hemostasis, Protein S exhibits diverse non-hemostatic functions, primarily through interactions with cellular receptors and complement components, contributing to immune regulation and tissue homeostasis.^[54] Protein S facilitates the clearance of apoptotic cells by binding to exposed phosphatidylserine on their surfaces, thereby promoting phagocytosis by macrophages. This process is mediated by the sex hormone-binding globulin-like region of Protein S, which engages Tyro3, Axl, and Mer (TAM) family receptor tyrosine kinases on phagocytes to enhance engulfment and prevent secondary necrosis or inflammation. Studies have demonstrated that purified Protein S directly stimulates macrophage uptake of apoptotic lymphocytes, with immunodepletion of Protein S abolishing this serum-dependent activity.^[55]^[54] In addition to apoptotic clearance, Protein S exerts anti-inflammatory effects by modulating endothelial responses and complement activation. It inhibits the expression of tissue factor on endothelial cells, reducing procoagulant and proinflammatory signaling in response to stimuli like tumor necrosis factor-alpha. Protein S also regulates vascular permeability by activating p38 mitogen-activated protein kinase (MAPK) and Rho/ROCK signaling pathways in endothelial cells, influencing barrier function and inflammation.^[2] Furthermore, when complexed with C4b-binding protein (C4BP), Protein S regulates the classical complement pathway, limiting amplification of inflammatory cascades while preserving its free form for other cellular interactions. Protein S promotes fibrinolysis independently by acting as a cofactor for APC in the neutralization of plasminogen activator inhibitor-1 (PAI-1), enhancing tissue plasminogen activator-mediated clot dissolution.^[56] These actions collectively dampen excessive immune responses in various tissues.^[57]^[58]^[54] Structurally homologous to growth arrest-specific 6 (Gas6), Protein S shares vitamin K-dependent gamma-carboxylation and serves as a ligand for TAM receptors, though with distinct binding affinities. While Gas6 potently activates all three TAM receptors to promote cell survival, proliferation, and migration, Protein S primarily binds weakly to Tyro3, eliciting more selective signaling for anti-apoptotic effects in certain cell types, such as endothelial cells and neurons. This homology underscores Protein S's contributions to cellular protection and homeostasis independent of coagulation.^[57]^[59] During pregnancy, Protein S levels fluctuate, with free Protein S decreasing from the 10th gestational week and total antigen from the 20th, influenced by rising C4BP and gestational hormones. Protein S localizes to the fetomaternal interface, particularly in degenerative placental villi, suggesting a protective role in restoring or safeguarding damaged trophoblast cells through anti-apoptotic and anti-inflammatory mechanisms beyond anticoagulation. This deposition may support placental integrity and fetal development.^[60]

Clinical Significance

Deficiency and Pathology

Protein S deficiency can be hereditary or acquired, both leading to impaired anticoagulant function and heightened thrombotic risk. Hereditary protein S deficiency is an autosomal dominant disorder caused by heterozygous mutations in the PROS1 gene, with an estimated prevalence of 0.03% to 0.13% in healthy populations. It is classified into three types: type I, characterized by reduced levels of both total and free protein S antigen with decreased activity; type II, a qualitative defect with normal antigen levels but impaired activity; and type III, featuring normal total protein S but low free protein S levels and reduced activity. Heterozygous individuals face a 6- to 8-fold increased lifetime risk of venous thromboembolism (VTE), including deep vein thrombosis (DVT) and pulmonary embolism (PE), with approximately 50% developing at least one event, often by median age 29 years. This risk is further amplified by provoking factors such as pregnancy or oral contraceptive use, which can exacerbate the deficiency. Acquired protein S deficiency arises from secondary conditions that disrupt synthesis, consumption, or clearance of the protein, without underlying genetic mutations. Common causes include liver disease, which impairs hepatic production; nephrotic syndrome, leading to urinary loss; disseminated intravascular coagulation (DIC), causing consumption; and vitamin K antagonists like warfarin, which interfere with gamma-carboxylation. During the COVID-19 pandemic, studies from 2020 to 2021 reported associations with reduced protein S levels, often due to degradation or consumption, with hospitalized patients showing approximately 20% to 30% drops in activity compared to healthy controls, correlating with disease severity. These reductions contribute to the hypercoagulable state observed in severe cases. The pathology of protein S deficiency manifests primarily as thrombophilia, predisposing individuals to VTE events like DVT and PE, though arterial thrombosis shows no strong link, with only minimal evidence of association. In women, it is linked to recurrent miscarriages, with an odds ratio of about 3.3 for late fetal loss, potentially due to placental thrombosis. Severe homozygous forms, rare in heterozygotes but possible in compound states, present in neonates as purpura fulminans, a life-threatening condition involving widespread dermal thrombosis, skin necrosis, and disseminated intravascular coagulation. Recent genome-wide association studies (GWAS) from 2022 to 2024 have identified PROS1 variants as contributors to thrombotic risk in multi-ethnic cohorts. A 2024 exome sequencing analysis in the UK Biobank (349,038 participants, including diverse ancestries such as Asian, Black, and mixed) found protein-coding variants in PROS1 associated with VTE (odds ratio 1.08, p = 1.31 × 10⁻⁹). Similarly, a 2025 multipopulation GWAS meta-analysis across nine cohorts (over 1 million participants) prioritized PROS1 as a causal gene for VTE, supported by functional evidence from zebrafish models showing altered thrombin activity.

Diagnosis and Measurement

Diagnosis of Protein S (PS) deficiency typically involves laboratory assessment of PS levels and function in plasma, with testing recommended in select clinical scenarios such as unprovoked venous thromboembolism (VTE) in young individuals or family history of thrombophilia.^[61] The primary assays measure PS antigen and activity, distinguishing between total PS (free plus C4b-binding protein [C4BP]-bound forms) and free PS, as only the free form exhibits anticoagulant activity.^[1] Immunoassays, particularly enzyme-linked immunosorbent assays (ELISA) or latex particle-enhanced immunoturbidimetric assays using monoclonal antibodies, quantify total and free PS antigen levels.^[61] Normal total PS antigen levels in adults range from 60 to 113 IU/dL, while free PS antigen levels are typically 58 to 166 IU/dL in males and 58 to 125 IU/dL in females, though reference intervals must be established by individual laboratories accounting for age, sex, and ethnicity.^[1]^[62] Free PS antigen measurement is the preferred initial test due to its specificity for functional PS and lower variability compared to total antigen assays.^[61] Functional assays evaluate PS anticoagulant activity, often serving as a confirmatory step after antigen testing or in cases of suspected type II deficiency where antigen levels are normal but function is impaired.^[61] Clot-based methods, such as those prolonging prothrombin time (PT) or activated partial thromboplastin time (APTT) in the presence of activated protein C (APC), or chromogenic assays using factor Xa or Russell's viper venom time, are commonly employed to measure PS activity.^[61] These assays report activity as a percentage of normal plasma, with adult reference ranges generally 60% to 150% for males and 50% to 150% for premenopausal females, varying by method and population.^[63]^[64] According to the International Society on Thrombosis and Haemostasis (ISTH) Scientific and Standardization Committee (SSC) recommendations, free PS antigen assays are prioritized as the first-line diagnostic tool, with functional assays recommended for subtyping deficiencies or when antigen results are equivocal.^[61] Several challenges complicate PS testing, including physiological and pathological interferences that can lead to false lows or highs. Elevated C4BP levels, which bind up to 60% of circulating PS, reduce free PS measurements, while conditions like pregnancy and oral contraceptive use decrease free PS by 20-50% due to estrogen-induced C4BP upregulation.^[1]^[65] Other interferences include lupus anticoagulant, direct oral anticoagulants (DOACs), heparin, and factor V Leiden, which can artifactually lower activity in clot-based assays, with specificity ranging from 40-70%.^[61] The ISTH SSC guidelines (2022) advise deferring testing during acute thrombosis, pregnancy, or anticoagulation therapy, recommending repeat confirmation at least 4 weeks after the initial abnormal result and avoidance of testing in the first 6 months postpartum.^[61] In suspected hereditary PS deficiency, genetic screening targets the PROS1 gene using next-generation sequencing (NGS) panels to identify pathogenic variants after biochemical confirmation.^[66] NGS detects single nucleotide variants and copy number changes in PROS1, which accounts for most hereditary cases, but is not recommended for routine prevalence screening due to low population yield and availability primarily through specialized or research laboratories.^[66]^[61] The ISTH emphasizes genetic testing in familial cases or young patients with unprovoked VTE to guide counseling, with over 60% of confirmed hereditary deficiencies attributable to PROS1 mutations.^[67]

Treatment and Management

The management of Protein S deficiency primarily focuses on preventing and treating venous thromboembolism (VTE) events, with strategies tailored to the type (congenital or acquired), severity, and patient-specific risk factors such as prior VTE history.^[3] For individuals with congenital Protein S deficiency, lifelong anticoagulation is often recommended for those with severe deficiency or recurrent thrombosis to mitigate the elevated risk of VTE recurrence.^[68] Prophylactic anticoagulation with low-molecular-weight heparin (LMWH), such as enoxaparin, is standard in high-risk situations including major surgery, prolonged immobilization, and pregnancy, where it is typically administered throughout gestation and extended for 6 weeks postpartum to reduce thrombotic complications.^[69]^[3] Replacement therapy is reserved for rare, severe cases or acute thrombotic events unresponsive to anticoagulation, though options are limited due to the lack of widely available specific Protein S concentrates. Fresh frozen plasma (FFP) can provide temporary Protein S supplementation in emergencies, such as neonatal purpura fulminans associated with homozygous deficiency, but its use is off-label and requires careful monitoring for volume overload.^[3] For acquired Protein S deficiency linked to vitamin K antagonism or deficiency, supplementation with vitamin K (typically 5-10 mg orally or intravenously) restores levels by supporting gamma-carboxylation of the vitamin K-dependent protein.^[70] In acute VTE treatment, initial therapy involves LMWH or unfractionated heparin bridged to oral anticoagulants like warfarin (target INR 2.0-3.0) or direct oral anticoagulants (DOACs) for at least 3-6 months, with extension based on recurrence risk.^[69]^[71] Current guidelines from the American College of Chest Physicians (ACCP, 2021, with 2024 updates) advise against routine thrombophilia screening, including for Protein S deficiency, in most VTE patients due to limited impact on management decisions, but endorse individualized therapy for those with confirmed deficiency and VTE history, favoring extended anticoagulation over 3 months for unprovoked events.^[72]^[73] The American Society of Hematology (ASH) 2020 guidelines similarly recommend against routine testing but support prophylaxis in high-risk scenarios like pregnancy for women with Protein S deficiency and a family history of VTE.^[74] For acquired deficiencies associated with COVID-19, where inflammation induces Protein S drops exacerbating coagulopathy, anti-inflammatory agents like interleukin-6 inhibitors (e.g., tocilizumab) have been explored to mitigate endothelial dysfunction and restore anticoagulant balance, though specific trials for Protein S modulation remain ongoing.^[75]^[76]

Interactions

Protein-Protein Interactions

Protein S forms a high-affinity, non-covalent complex with the β-chain of C4b-binding protein (C4BP) in a 1:1 molar ratio, with a dissociation constant (Kd) of approximately 1 nM in the presence of calcium ions. This interaction primarily involves the short consensus repeat 1 (SCR-1) domain of the C4BP β-chain and residues in the sex hormone-binding globulin (SHBG)-like region of Protein S, such as amino acids 420-434 and 605-615. Under physiological conditions, this binding sequesters approximately 60% of circulating Protein S, thereby reducing its free form and modulating its anticoagulant activity by preventing it from serving as a cofactor for activated protein C (APC). Affinity studies using chimeric constructs and mutagenesis approaches in the 1990s, including co-immunoprecipitation assays, confirmed the critical role of SCR-1 and adjacent SCR-2 in enhancing binding stability.^[77]^[78]^[12] In the coagulation cascade, Protein S interacts with APC to enhance its proteolytic inactivation of Factors Va and VIIIa on phospholipid surfaces. This cofactor function is mediated through an exosite on APC that promotes Protein S binding, increasing APC's affinity for negatively charged membranes by approximately twofold independently and over 14-fold in synergy with Factor Va. Protein S also directly binds Factor Va via residues 37-50 in its Gla-thrombin-sensitive region (Gla-TSR) domain and 621-635 in the SHBG-like region, facilitating substrate presentation for APC cleavage at Arg-306 while inhibiting prothrombinase complex assembly. Similarly, Protein S binds Factor VIIIa to promote its APC-mediated degradation, exerting a moderate 1.5-fold enhancement alone but up to 11-fold synergistically with Factor V as a phospholipid-bound cofactor. These interactions were delineated through biochemical assays and mutagenesis studies spanning the 1990s to 2010s.^[12]^[79]^[80] Beyond coagulation, Protein S acts as a ligand for the Tyro3 receptor tyrosine kinase, exhibiting low-affinity binding with species-specific characteristics—human Protein S shows weak affinity for human Tyro3, unlike bovine Protein S. This interaction occurs via the SHBG domain of Protein S and the extracellular Ig-like domains of Tyro3, triggering downstream signaling through the PI3K-Akt pathway to support anti-inflammatory responses and efferocytosis. Seminal studies in the 1990s identified Protein S as a functional Tyro3 ligand, with subsequent work in the 2010s confirming its role in neuronal protection and signaling specificity.^[81]^[82]^[83] Through its association with C4BP, Protein S indirectly interacts with complement system components, such as C4b, facilitating the recruitment of the C4BP complex to apoptotic cells or activated platelets to dampen complement activation and inflammation. This linkage bridges coagulation and complement regulation, as evidenced by phospholipid-binding assays in the early 2000s.^[12] The papain-like protease (PLpro) of SARS-CoV-2 cleaves human Protein S (PROS1) at an internal site within the sequence IYHSAWLLIALRGG, generating fragments of approximately 39.9 kDa and 35.3 kDa that impair its anticoagulant function.^[84] This cleavage disrupts the protein's ability to serve as a cofactor for activated protein C (APC), contributing to the dysregulated coagulation observed in infected individuals. In vitro assays confirm that PLpro-mediated proteolysis occurs efficiently, and zinc ions can inhibit this process, highlighting a potential therapeutic target.^[84] In patients with severe COVID-19, free Protein S levels are significantly reduced, with studies reporting decreases of approximately 20-25%, particularly in those requiring oxygen therapy, due to the prevalence of its free form (30–40% of total circulating Protein S) being preferentially cleaved by viral PLpro.^[85] This deficiency correlates with hypercoagulability and has been linked to thrombotic microangiopathy, a hallmark of severe disease characterized by microvascular clotting and organ damage. Recent investigations, including 2025 studies, suggest that modulating PROS1 expression in lung basal cells could limit epithelial inflammation and mitigate coagulopathic complications during SARS-CoV-2 infection.^[86] Beyond SARS-CoV-2, HIV infection is associated with acquired Protein S deficiency, which impairs the APC pathway and promotes a prothrombotic state, potentially exacerbating coagulopathy in sepsis-like conditions among affected patients.^[87] For bacterial pathogens, Staphylococcus aureus recruits the complement regulator C4b-binding protein (C4BP) to its surface via proteins such as SdrE and Bbp, which inhibits opsonization and bacterial killing.^[88] Since C4BP forms complexes with Protein S in plasma, this recruitment indirectly sequesters Protein S, potentially reducing its availability for anticoagulant activity and aiding S. aureus evasion of host defenses during infection.

References

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[5]
Protein S - DiaPharma
Protein S is a vitamin K-dependent plasma glycoprotein that acts as a cofactor in the anti-coagulation pathway, regulating blood coagulation.The Coagulation Cascade · Domain Structure · Protein S Assays Methods
[6]
Anticoagulant protein S—New insights on interactions and functions
Protein S was first isolated and characterized in 1977 and since then ... This was followed by the discovery of protein S functioning as a cofactor in ...
[7]
Protein S deficiency: a clinical perspective - Wiley Online Library
Oct 30, 2008 · Summary. Protein S (PS) is an extensively studied protein with an important function in the down-regulation of thrombin generation.
[8]
Domain Evolution of Vertebrate Blood Coagulation Cascade Proteins
Oct 1, 2022 · In this study, we examined proteomes of 21 chordates, of which 18 are vertebrates, to reveal the modular evolution of the blood coagulation cascade.Results And Discussion · Arthropod Hemolymph Clotting · Origin Of Domains And...
[9]
Functional properties of the sex‐hormone‐binding globulin (SHBG ...
Jan 15, 2003 · Protein S (PS) possesses a sex-hormone-binding globulin (SHBG)-like domain in place of the serine-protease domain found in other vitamin K ...Missing: distinction | Show results with:distinction
[10]
Crystal structure of human sex hormone‐binding globulin: steroid ...
Figure 1. Modular architecture of SHBG and its homologues GAS6, protein S and laminin. The SHBG‐like domain consists of a tandem repeat of laminin G‐like ...
[11]
Entry - *176880 - PROTEIN S; PROS1 - OMIM - (OMIM.ORG)
(1990) determined that the PROS1 gene contains 15 exons and spans more than 80 kb. ... Two genes homologous with human protein S cDNA are located on chromosome 3.
[12]
Inherited protein S deficiency due to a novel nonsense mutation in ...
PROS1 is located near the centromere on chromosome 3q11.2 [6]. So far, almost 200 different PROS1 mutations resulting in a loss of function have been identified ...
[13]
A thrombophilia family with protein S deficiency due to protein ...
Sep 8, 2021 · The analysis of family phenotype, gene association, and cell function tests suggest that the PROS1 Leu607Ser heterozygous mutation may be a pathogenic mutation.Clinical Phenotype Detection · Clinical Phenotypes · Cloning Of Pros1 Wt And P...Missing: Cys414Phe | Show results with:Cys414Phe
[14]
Venous thromboembolism associated with protein S deficiency due ...
Here, we report the first Polish case with PS deficiency caused by the p.Arg451* in the PROS1 gene detected in a 21-year-old man with trauma-induced venous ...Missing: Cys414Phe HGMD 200
[15]
Genetic Variants in the Protein S ( PROS1 ) Gene and ... - PMC - NIH
Oct 28, 2021 · Protein S (PS) deficiency is a risk factor for venous thromboembolism (VTE) and can be caused by variants of the gene encoding PS ( PROS1 ).Results · Pros1 Variants And... · Pros1 Variants And Venous...Missing: Cys414Phe HGMD
[16]
Inherited Thrombophilias - DynaMed
Prevalence of protein S deficiency: The prevalence in the general population: Protein S deficiency is reported in 0.03%-0.13% of the general population ...
[17]
Protein S Deficiency and the Risk of Venous Thromboembolism in ...
Jun 23, 2022 · After adjusting for age and gender, the odds ratio of developing venous thromboembolism in individuals with protein S deficiency based on free ...
[18]
Protein C and Protein S Deficiencies Are the Most Important Risk ...
Only PC and PS deficiencies were significantly associated with increased risk for the development of thrombosis with an OR of 10.6 and 6.7, respectively. The ...Missing: thromboembolism | Show results with:thromboembolism
[19]
Multiplex Ligation Probe Amplification and Sanger Sequencing - NIH
Apr 16, 2025 · Later, an in-depth study using MLPA revealed a heterozygous deletion in exons 9 and 18. The studies were also extended to the proband's family ...
[20]
Hereditary protein S deficiency from a novel large deletion mutation ...
The authors herein describe our experience of detection of a large deletion mutation of PROS1 by the MLPA technique in a Korean patient with thrombophilia. We ...Missing: Sanger | Show results with:Sanger
[21]
https://pubmed.ncbi.nlm.nih.gov/29321366/
[22]
Human endothelial cells synthesize protein S - PubMed - NIH
Intrinsic labeling and immunoprecipitation indicated that protein S was synthesized and secreted as a 75,000 molecular weight protein. Vitamin K, phorbol ...Missing: extrahepatic megakaryocytes testes
[23]
Biosynthesis and secretion of functional protein S by a human ...
Jul 1, 1987 · Abstract. A human megakaryoblastic cell line (MEG-01) was investigated for the presence of protein S in culture medium and cell lysates ...
[24]
PROS1 Gene - GeneCards | PROS Protein | PROS Antibody
Additional Variant Information for PROS1 Gene. Human Gene Mutation Database (HGMD): PROS1; SNPedia medical, phenotypic, and genealogical associations of SNPs ...
[25]
Down-regulation of PROS1 Gene Expression by 17beta-estradiol ...
Apr 30, 2010 · Pregnant women show a low level of protein S (PS) in plasma, which is known to be a risk for deep venous thrombosis. 17Beta-estradiol (E(2)) ...Missing: VKORC1 cytokines
[26]
TNF-alpha suppresses IL-6 upregulation of protein S in HepG-2 ...
The results demonstrated that TNF-alpha, and to a lesser degree, IL-1 alpha, could significantly suppress IL-6 upregulation of protein S, whereas the effects ...Missing: synthesis downregulated
[27]
Acquired Deficiencies of Protein S. Protein S Activity During Oral ...
After the initiation of warfarin, the apparent half-life of protein S is 42.5 h. In patients with thromboembolic disease, transient protein S deficiency occurs ...
[28]
5627 - Gene ResultPROS1 protein S [ (human)] - NCBI
Aug 19, 2025 · The PROS1 gene encodes a vitamin K-dependent plasma protein that acts as a cofactor for activated protein C to inhibit blood coagulation.Missing: PROS2 | Show results with:PROS2
[29]
CALCIUM BINDING TO ANTICOAGULANT PROTEIN S. ROLE ... - NIH
Protein S Gla-domain mutations causing impaired Ca(2+)-induced phospholipid ... residues 1-85. Mutations V46E and Q50E each manifest a negligible ...
[30]
Crystal structure of human sex hormone-binding globulin - NIH
The SHBG-like domain consists of a tandem repeat of laminin G–like domains. GAS6 and protein S both contain an N–terminal GLA domain and four EGF-like domains.
[31]
C-terminal Residues 621â€“635 of Protein S Are Essential for ...
Jun 17, 1999 · Protein S is anticoagulant in the absence of activated protein C because of direct interactions with coagula- tion Factors Xa and Va.
[32]
Interaction between Protein S and Complement C4b-binding Protein ...
C4BP also has a high affinity for anticoagulant vitamin K-dependent protein S, and together they form a noncovalent 1:1 stoichiometric complex (8-11).Missing: monomer | Show results with:monomer
[33]
CRYSTAL STRUCTURE OF N-TERMINAL DOMAIN OF PROTEIN S
Protein S from Myxococcus xanthus is a member of the beta gamma-crystallin superfamily. Its N and C-terminal domains (NPS and CPS, respectively) show a high ...Missing: 2PSQ | Show results with:2PSQ
[34]
Protein S: Function, Regulation, and Clinical Perspectives - PMC
Protein S acts as an anticoagulant by performing three different functions: 1) Cofactor for APC, 2) Cofactor for TFPI, and 3) Inhibitor of FIXa.Missing: structure | Show results with:structure
[35]
Activated protein C cofactor function of protein S: a novel role for a γ ...
Jun 16, 2011 · Binding to negatively charged phospholipid surfaces is crucial for protein S to mediate its APC cofactor function. Protein S Face1 has ...
[36]
beta-Hydroxyasparagine in domains homologous to the epidermal ...
We now show that, in protein S, this EGF-like repeat has one beta-hydroxyasparagine residue formed by hydroxylation of asparagine. The two COOH-terminal EGF ...
[37]
Aspartate/asparagine-β-hydroxylase crystal structures reveal an ...
Oct 28, 2019 · AspH catalyses hydroxylation of asparaginyl- and aspartyl-residues in epidermal growth factor-like domains (EGFDs). Here we report crystal ...
[38]
Localization of Thrombin Cleavage Sites in the Amino-Terminal ...
Apr 15, 1986 · Thrombin cleaves two peptide bonds in this part of protein S, first at arginine 70 and then at arginine 52. The peptide containing residues 53- ...
[39]
https://pmc.ncbi.nlm.nih.gov/articles/PMC305588/
[40]
https://www.jbc.org/article/S0021-9258%2819%2953148-9/pdf
[41]
Free protein S levels are elevated in familial C4b-binding ... - PubMed
Dec 15, 1990 · In plasma, 40% of the protein S is free and functions as a cofactor for the anticoagulant effects of activated protein C. The remaining 60% ...
[42]
different roles for protein S and the protein S–C4b binding protein ...
Feb 15, 2004 · PS normally circulates in human plasma at a concentration of approximately 25 mg/L (0.30 μM). After posttranslational modifications, including γ ...
[43]
Resistance to activated protein C as risk factor for thrombosis
Activated protein C [APC] cleaves and inhibits membrane bound factor Va and factor VIIIa, which leads to specific and efficient downregulation of the ...
[44]
Vitamin K-Dependent Protein S: Beyond the Protein C Pathway
Protein S is a vitamin K-dependent plasma glycoprotein with anticoagulant properties. It affects C4BP, interacts with TAM receptors, and stimulates ...Missing: classification | Show results with:classification
[45]
Serum-derived protein S binds to phosphatidylserine and stimulates ...
Nov 25, 2002 · Figure 2: Protein S stimulates phagocytosis of apoptotic BL-41 cells by macrophages. ... Delayed apoptotic cell clearance and lupus-like ...
[46]
TAM Receptors, Gas6, and Protein S: Roles in Inflammation and ...
Apr 17, 2014 · When activated, the TAM receptors have effects on primary hemostasis and coagulation and display an anti-inflammatory or a proinflammatory ...
[47]
Regulation of inflammation by the protein C system - PubMed
Protein S not only suppresses coagulation as an enhancing cofactor for the coagulation inhibitors activated protein C and tissue factor pathway inhibitor but ...
[48]
Gas6 and Protein S. Vitamin K-dependent Ligands for the Axl ...
Gas6 and protein S are two homologous secreted proteins that depend on vitamin K for their execution of a range of biological functions.
[49]
a possible role of protein S other than anticoagulation - PubMed
Protein S levels are thought to decrease during pregnancy, but the underlying mechanism remains unknown. We compared protein S concentrations throughout normal ...
[50]
Recommendations for clinical laboratory testing for protein S ...
This report presents the current evidence‐based recommendations for clinical PS assays as well as when to test for PS abnormalities.
[51]
Free protein S antigen - Synnovis |
Oct 4, 2023 · Reference range: Male = 64.0 to 166.0 IU/dL; Female = 58.0 to 125.0 IU/dL. Synonyms or keywords: FPS Ag, protein S. Units: iu/dL. Department ...Missing: U/ | Show results with:U/
[52]
Protein S Activity | Test Detail | Quest Diagnostics
Reference Range(s). Male, 70-150 % normal. Female, 60-140 % normal. Alternative Name(s). Protein S, Functional. LOINC® Codes, Performing Laboratory. Service ...
[53]
Protein S Activity [not a screening test] - MLabs
Male: 65 - 150%, Female <50 years: 50 - 150, Female > or =50 years: 65 - 150%. Newborn infants have normal or near-normal free protein S antigen (> or =50%), ...
[54]
Oral contraceptives and gender affect protein S status - PubMed - NIH
The results show that women taking oral contraceptives have significantly lower total protein S (24.3 +/- 3.6 micrograms/mL; mean +/- SD) than women not taking ...Missing: testing interferences C4BP ISTH
[55]
GNPRS - Overview: Protein S Deficiency, PROS1 Gene, Next ...
This test utilizes next-generation sequencing to detect single nucleotide and copy number variants in the PROS1 gene associated with thrombophilia due to ...Missing: PTM | Show results with:PTM
[56]
PROTEIN S DEFICIENCY - Medicover Genetics
Congenital protein S deficiency is classified as follows: Type I: quantitative defect, decreased total and free protein S and protein S activity; Type II ...
[57]
Thrombophilia: Deficiencies in Protein C, Protein S and Antithrombin
Nov 2, 2025 · Underlying Medical Conditions: Various conditions—including liver disease, severe vitamin K deficiency, DIC, nephrotic syndrome, and certain ...
[58]
Protein S Deficiency Treatment & Management - Medscape Reference
Jan 31, 2023 · Main agents in the acute period include intravenous unfractionated heparin, low molecular weight heparin (LMWH), or a direct oral anticoagulant (DOAC).
[59]
Protein S Deficiency - Hematology and Oncology - Merck Manuals
Protein S deficiency can also occur when patients use estrogen ; replacement therapy or contraception and during pregnancy due to the influence of estrogen ; on ...
[60]
Protein S deficiency: a clinical perspective - Blood Clots
May 8, 2013 · Protein S (PS) is an extensively studied protein with an important function in the down-regulation of thrombin generation.Protein S Deficiency: A... · Types Of Ps Deficiency · Genetic/molecular Basis Of...
[61]
CHEST releases new guidelines for antithrombotic therapy for VTE ...
Aug 3, 2021 · The American College of Chest Physicians (CHEST) recently released new clinical guidelines for venous thromboembolism (VTE) management.Missing: Protein deficiency
[62]
Venous Thromboembolism (VTE) Guidelines - Medscape Reference
2021 ACCP (CHEST) Guidelines for Venous Thromboembolism. The second update on the ninth edition of the initial 2016 American College of Chest Physicians (CHEST) ...
[63]
ASH VTE Guidelines: Thrombophilia Testing
The purpose of these guidelines is to provide evidence-based recommendations about whether thrombophilia testing and tailoring management based on the test ...
[64]
New plasma protein C and protein S concentrate: A synergy for ...
Nov 28, 2023 · First-line treatment involves replacement therapy, followed by maintenance with anti-coagulants. Replacement therapy with specific protein ...
[65]
Dysregulation of Protein S in COVID-19 - PMC - PubMed Central
Acquired PS deficiency is common in patients with severe viral infections and has been reported in multiple studies of COVID-19.
[66]
IDSA Guidelines on the Treatment and Management of Patients with ...
We recommend using either IL-6 inhibitors or JAK inhibi-tors (baricitinib preferred over tofacitinib) in those patients who have elevated inflammatory markers ...
[67]
https://medicover-genetics.com/product/protein-s-deficiency/
[68]
Interaction between protein S and complement C4b ... - PubMed
May 21, 1999 · C4BP can bind anticoagulant protein S, resulting in a decreased cofactor function of protein S for activated protein C. C4BP is a multimeric ...Missing: yeast hybrid
[69]
Factor V and protein S as cofactors to activated protein C - PubMed
Factor V and protein S act as cofactors to activated protein C (APC) in the degradation of factor VIIIa, working in synergy as phospholipid-bound cofactors.
[70]
The Journey of Protein S from an Anticoagulant to a Signaling ... - NIH
In 1977, while purifying Protein C from bovine plasma investigators discovered a new γ-carboxyglutamate (Gla)- containing protein which they named Protein S (PS) ...Missing: Earl University Washington
[71]
TAM receptors, Gas6, and protein S: roles in inflammation and ...
Protein S binding to Tyro3 and Mer shows a high degree of species specificity. Peculiar is that human protein S shows only weak or no affinity for the ...Introduction · Protein S · TAM receptors · Knockout mice
[72]
https://www.chestnet.org/newsroom/press-releases/2021/08/chest-releases-new-guidelines-for-antithrombotic-therapy-for-vte-disease
[73]
PROS1 released by lung basal cells limits inflammation in epithelial ...
Aug 27, 2025 · We hypothesized that PROS1 may protect the upper airways by regulating epithelial and myeloid cell responses during severe acute respiratory ...
[74]
Cardiovascular Disease and Thrombosis in HIV Infection
Dec 1, 2022 · There is also evidence of protein S autoantibodies in PLWH, which may contribute to protein S deficiency. PLWH also have antiphospholipid ...Hiv Infection And Modern... · Potential Molecular... · Venous Thrombosis