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Strigolactone

Strigolactones (SLs) are a class of carotenoid-derived plant hormones that regulate diverse aspects of plant growth, development, and environmental interactions.^[1] Characterized by a conserved chemical structure consisting of a tricyclic lactone moiety (ABC rings) connected via an enol-ether bridge to an α,β-unsaturated furanone (D ring), SLs exhibit structural diversity across species, with over 30 variants identified, including canonical forms like strigol and orobanchol.^[2] Initially discovered in the 1960s as root exudates stimulating seed germination in parasitic plants such as Striga and Orobanche, SLs were later recognized in the 2000s as endogenous hormones inhibiting shoot branching in plants like pea, rice, and Arabidopsis.^[1] Their biosynthesis begins in plastids from β-carotene through sequential actions of enzymes including DWARF27 (D27, an isomerase), carotenoid cleavage dioxygenases 7 and 8 (CCD7 and CCD8), yielding the central precursor carlactone, which is then modified by cytochrome P450 monooxygenases like MAX1/CYP711A into bioactive SLs.^[2] As hormones, SLs primarily suppress axillary bud outgrowth to control shoot architecture, integrating with auxin and cytokinin signaling pathways to fine-tune branching in response to environmental cues like nutrient availability.^[1] They also promote root hair elongation and lateral root formation, enhancing nutrient and water uptake, particularly under phosphate or nitrogen deficiency, where SL levels can increase dramatically—up to 20-fold in some species.^[2] Beyond development, SLs play crucial roles in stress resilience, interacting with abscisic acid (ABA) to mediate stomatal closure and gene expression for drought and salinity tolerance, while delaying leaf senescence to maintain photosynthetic efficiency.^[1] In the rhizosphere, SLs function as signaling molecules, inducing hyphal branching in arbuscular mycorrhizal (AM) fungi to promote symbiotic associations that improve plant nutrient acquisition, a role tracing back to the common ancestor of land plants over 400 million years ago.^[3] This dual hormonal and ecological function underscores SLs' evolutionary significance, with biosynthesis genes like CCD8 and MAX1 conserved in AM-forming species but absent in non-symbiotic ones.^[3] In agriculture, SLs offer potential for crop improvement by enhancing stress tolerance and symbiosis, though their stimulation of parasitic weeds poses challenges for weed management.^[1]

Introduction and History

Definition and Overview

Strigolactones (SLs) are a class of terpenoid lactone phytohormones derived from carotenoids, initially identified as signaling molecules in the plant rhizosphere.^[4] These compounds are produced primarily in roots and exuded into the soil, where they serve dual roles as endogenous hormones regulating plant development and as external signals influencing interactions with soil microbes and parasites.^[5] SLs are biosynthesized from the carotenoid pathway, highlighting their evolutionary conservation across diverse plant species.^[6] SLs play key roles in internal plant architecture by inhibiting shoot branching and promoting axillary bud dormancy, thereby optimizing resource allocation under varying conditions.^[7] Externally, they stimulate the germination of seeds from root parasitic plants such as Striga and Orobanche species, which pose significant agricultural threats, while also promoting symbiotic associations with arbuscular mycorrhizal fungi (AMF) to enhance nutrient uptake, particularly phosphorus.^[8] This dual functionality underscores SLs' importance in both developmental control and ecological interactions in the rhizosphere.^[8] SLs are classified into canonical forms, which feature a conserved ABCD ring structure (e.g., strigol and orobanchol), and non-canonical forms that lack certain rings but retain the bioactive D-ring moiety (e.g., carlactonoic acid).^[9] Canonical SLs are typically more potent in stimulating parasitic seed germination, whereas non-canonical variants often predominate in hormonal regulation of shoot architecture.^[8] Overall, SLs integrate environmental cues, such as nutrient availability, with developmental responses to fine-tune plant growth and adaptation.^[7]

Discovery and Early Characterization

Strigolactones were first discovered in 1966 when Cook et al. isolated strigol, a crystalline compound from root exudates of cotton (Gossypium hirsutum), demonstrating its potent stimulatory effect on seed germination of the parasitic weed witchweed (Striga lutea) at concentrations below 10^{-5} parts per million.^[10] This isolation marked the initial recognition of strigolactones as key signaling molecules in plant-parasite interactions, achieved through extraction of root exudates collected from hydroponically grown plants and purification via chromatography, followed by bioassays measuring germination rates of Striga seeds.^[10] Early characterization relied heavily on such bioassays, where root exudates or purified fractions were tested for their ability to induce germination in dormant parasitic seeds, highlighting strigol's hormonal-level activity.^[11] In the 1970s and 1980s, research expanded on strigolactone-like compounds from various host plants, primarily aimed at understanding and mitigating parasitic weed infestations in agriculture. Investigations focused on genuine hosts like sorghum (Sorghum bicolor), where root exudates were analyzed for germination stimulants of Striga asiatica and Striga hermonthica using similar bioassay-driven isolation techniques, including solvent extraction and thin-layer chromatography.^[11] A notable advancement came in 1992 with the isolation of sorgolactone from sorghum root exudates, identified as a strigol analogue through spectroscopic methods and confirmed via its efficacy in Striga seed germination assays, underscoring the diversity of these compounds across species for potential weed control strategies.^[11] These efforts emphasized the ecological role of strigolactones in stimulating parasitic weed germination, with studies exploring synthetic analogues to disrupt parasite-host dynamics without delving into endogenous plant functions.^[11] A paradigm shift occurred in 2008–2009 when strigolactones were identified as endogenous shoot branching inhibitors, transforming their perceived role from mere ecological signals to plant hormones.^[12]^[13] This revelation stemmed from analyses of branching mutants, such as more axillary growth (max) in Arabidopsis thaliana, ramosus (rms) in pea (Pisum sativum), and decreased apical dominance (dad) in petunia (Petunia hybrida), where reduced strigolactone levels correlated with excessive branching; application of synthetic strigolactone GR24 rescued the wild-type phenotype in these mutants. Key studies by Umehara et al. (2008) demonstrated that strigolactone deficiency in rms1 and max4 mutants underlies their branching phenotype, while Booker et al. (2009) confirmed the hormonal function through grafting experiments and metabolite profiling.^[12]^[13] Branching bioassays, involving measurement of axillary bud outgrowth in response to hormone treatments, complemented traditional germination tests during this period. During the 2010s, structural elucidation advanced with the identification of carlactone in 2014 as the core biosynthetic precursor to strigolactones, produced via sequential action of carotenoid cleavage dioxygenases (CCD7 and CCD8) and cytochrome P450 (MAX1) on β-carotene in vitro, verified through enzymatic assays and LC-MS analysis.^[14] Strigolactones have been identified in non-vascular plants like mosses (e.g., Physcomitrella patens), where they regulate protonemal branching, extending their evolutionary origins through genomic and metabolomic studies.^[15]^[3] These findings relied on advanced isolation methods, including targeted metabolite extraction from primitive plant tissues and hypersensitive bioassays for fungal hyphal branching.^[3]

Chemical Structure and Properties

Molecular Structures

Strigolactones (SLs) are characterized by a core molecular architecture consisting of a tricyclic lactone system comprising rings A, B, and C, connected via an enol ether bridge to a butenolide ring D. This strigol scaffold forms the basis for canonical SLs, where the ABC portion typically features a lactone ring at C and varying substituents on the A ring, such as hydroxyl or acetoxy groups, while the D ring maintains a specific α,β-unsaturated γ-butyrolactone motif essential for biological recognition.^[16]^[17] Among canonical SLs, strigol, isolated from root exudates of millet species such as Sorghum bicolor, represents the prototypical structure with a hydrogen at the C-4 position of the A ring and a double bond configuration that defines the strigol family. Orobanchol, identified in hosts of the parasitic plant Orobanche, features a hydroxyl group at C-4, distinguishing the orobanchol family prevalent in dicots. The synthetic analog GR24, with methyl and acetate substituents mimicking natural variants, is widely used in research due to its stability and activity across species. A key structural feature influencing bioactivity is the stereochemistry at the C-2' position of the enol ether bridge, where the 2'R configuration is predominant in natural SLs and enhances signaling efficacy in symbiotic and parasitic interactions.^[18]^[19]^[20] Non-canonical SLs deviate from the full tricyclic ABC system, often featuring an open or modified BC ring structure derived from biosynthetic precursors. Carlactonoic acid, an oxidized form of carlactone, lacks the closed A ring but retains the enol ether-linked D ring, serving as a key intermediate with bioactivity in certain contexts. Methyl carlactonoate, its methylated derivative, exhibits similar modifications and has been detected in various plant species. Variations in substituents, such as hydroxy or methoxy groups at C-4 or additional hydroxylations on the B ring, contribute to this diversity, with structures elucidated primarily through nuclear magnetic resonance (NMR) and mass spectrometry (MS) analyses.^[21]^[22] Nomenclature for SLs is typically derived from the plant source or associated function, such as "strigol" combining Striga (a parasitic genus) and "lactone," or "orobanchol" from Orobanche. By 2025, over 35 natural SLs have been identified across diverse plant species, categorized into canonical (closed ABC-D) and non-canonical forms based on ring integrity. This structural diversity, including family-specific modifications like the C-4 hydroxy in orobanchol (common in dicots) versus the unsubstituted A ring in strigol (prevalent in monocots), enables species-specific signaling in ecological interactions.^[23]^[24]

Physical and Chemical Properties

Strigolactones exhibit lipophilic characteristics, with calculated logP values typically ranging from 3 to 4 for natural compounds and their close analogs, such as 3.47 for Abz-E2B and 4.02 for Abz-E1.^[25] This lipophilicity contributes to their partitioning in plant tissues and soil environments, facilitating interactions with biological membranes. Their water solubility is low, generally in the range of 10–50 μM, which limits their persistence in aqueous media and often requires organic solvents like acetone or ethyl acetate for experimental handling.^[26] Additionally, strigolactones display characteristic UV absorption at 230–240 nm, attributable to the conjugated enol ether moiety in their structure.^[27] Chemically, strigolactones are unstable under environmental stresses, showing sensitivity to light, varying pH levels, and oxidizing agents, with primary degradation occurring via hydrolysis of the butenolide ring.^[9] In soil, their half-life varies from days to weeks depending on conditions; for instance, the synthetic analog GR24 persists for 1–3 days in alkaline soils (pH >7.5) but extends to 6–8 days in acidic soils (pH 5.0–6.3).^[28] Under neutral pH, the half-life of 5-deoxystrigol, a relatively stable canonical strigolactone, is approximately 1.5 days in dilute methanol solutions.^[29] The reactivity of strigolactones centers on the enol ether linkage, which undergoes nucleophilic attack by receptor proteins such as D14, leading to covalent adduct formation and signal initiation.^[9] This process is stereospecific, with (+)-stereoisomers—particularly those featuring the 2′R configuration—demonstrating higher bioactivity in germination stimulation and branching inhibition compared to their enantiomers.^[30] For analytical purposes, strigolactones are commonly detected using high-performance liquid chromatography-mass spectrometry (HPLC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) in multiple reaction monitoring mode, enabling sensitive quantification at picomolar to nanomolar levels in root exudates.^[29] These compounds have low volatility, but are exuded into the rhizosphere along with other organic compounds that protect them from rapid degradation and improve stability.^[26]

Biosynthesis

Carotenoid-Derived Pathway

Strigolactones (SLs) are derived from carotenoids through a conserved biosynthetic pathway that occurs primarily in plastids and the endoplasmic reticulum of plant cells. The pathway begins with β-carotene, a C40 tetraterpenoid carotenoid, which serves as the universal starting substrate. This process involves sequential enzymatic cleavages and oxidations to produce carlactone, a central intermediate that structurally resembles SLs and acts as the precursor for all canonical SLs. The pathway has been elucidated in model plants such as Arabidopsis thaliana and rice (Oryza sativa), where genetic studies of branching mutants identified key enzymes.^[31] The initial steps occur in plastids and require three enzymes to generate carlactone from β-carotene. First, the carotenoid isomerase D27 (also known as OsD27 in rice) converts all-trans-β-carotene to 9-cis-β-carotene by isomerizing the bond at the C9-C10 position. Next, the carotenoid cleavage dioxygenase 7 (CCD7, encoded by MAX3/RMS5/DAD1 in Arabidopsis and D17 in rice) performs the first oxidative cleavage, yielding 9-cis-10-apo-β-carotenal, a C19 aldehyde intermediate. Subsequently, CCD8 (encoded by MAX4/RMS1/DAD3 in Arabidopsis and D10 in rice) catalyzes a second cleavage and intramolecular rearrangement of 9-cis-10-apo-β-carotenal to form carlactone, introducing the characteristic butenolide ring essential for SL bioactivity. These reactions are stereospecific and conserved across vascular plants, with carlactone exported to the cytoplasm for further modification.^[31]^[32] In the cytoplasm, carlactone undergoes oxidation by cytochrome P450 monooxygenases to yield various SLs. In Arabidopsis, the cytochrome P450 enzyme MAX1 (CYP711A1), in conjunction with its reductase partner CPR1, performs three sequential oxidations at the C19 position of carlactone: first to 19-hydroxy-carlactone, then to 19-oxo-carlactone, and finally to carlactonoic acid. In Arabidopsis, carlactonoic acid is further modified, potentially via methylation to methyl carlactonoate and oxidation by the LBO enzyme, to yield non-canonical SLs such as CN1 and CN2. In contrast, in species like rice, additional cytochrome P450 enzymes in the CYP711 clan convert carlactone or intermediates to canonical SLs, such as ent-2'-epi-5-deoxystrigol.^[15] The overall pathway can be represented as:

\beta\text{-carotene} \xrightarrow{\text{D27}} 9\text{-cis-}\beta\text{-carotene} \xrightarrow{\text{CCD7}} 9\text{-cis-}10\text{-apo-}\beta\text{-carotenal} \xrightarrow{\text{CCD8}} \text{carlactone} \xrightarrow{\text{MAX1/CYP711 clan}} \text{SLs (canonical in grasses, non-canonical in Arabidopsis)}

While the carlactone route is universal for canonical SLs, branches to non-canonical SLs can occur via CCD8-independent paths in certain plants, involving alternative carotenoid cleavages or downstream modifications. For example, in rice, the cytochrome P450 OsCYP706C2 diverts carlactone to 4-oxo-19-hydroxy-carlactone, leading to the non-canonical SL 4-oxo-methyl carlactonoate (4-oxo-MeCLA), which promotes arbuscular mycorrhizal colonization.^[33]^[31]^[34] SL biosynthesis is organ-specific, occurring predominantly in roots to support symbiotic interactions and nutrient foraging, but also in shoots under environmental stress such as phosphate deficiency, where increased flux through the pathway enhances branching inhibition. In Arabidopsis and rice, MAX3/D17 and MAX4/D10 transcripts are highly expressed in roots, with lower levels in shoots that elevate under stress conditions. This spatial regulation ensures SLs act locally and systemically to coordinate plant architecture.^[32]^[35]^[36]

Regulation by Hormones and Environmental Factors

Strigolactone (SL) biosynthesis is tightly regulated by nutrient availability, with phosphate starvation serving as a key trigger for upregulation. Under low phosphate conditions, SL biosynthesis is upregulated in roots, with transcription factors like PHR1/PHR2 playing roles in phosphate starvation responses that enhance SL production to promote symbiotic associations. In rice, PHR2 directly binds to promoters of SL biosynthesis genes such as D27, D10, and D17, leading to increased SL levels.^[37] Similarly, nitrate availability modulates SL biosynthesis, with high nitrate often suppressing SL production under certain conditions to balance N-P acquisition, integrating nitrogen signaling with phosphate responses to fine-tune SL output. Abscisic acid (ABA) and SLs exhibit crosstalk during drought stress, with SLs promoting ABA-mediated stomatal closure and drought tolerance; while direct enhancement of SL biosynthesis by ABA is observed in some contexts, the primary interaction involves SL signaling supporting ABA responses.^[38] Environmental factors profoundly influence SL exudation and synthesis. Low phosphorus and nitrogen levels stimulate increased SL release from roots, enhancing signaling to arbuscular mycorrhizal fungi while adapting root architecture to nutrient scarcity; for instance, sorghum roots exude higher orobanchol under combined deficiencies.^[39] Light quality also modulates SL production, with red/far-red ratios sensed by phytochromes affecting shoot SL synthesis—far-red enrichment typically suppresses branching via elevated SL, independent of direct biosynthesis changes in some species like tomato.^[40] Developmental cues contribute to SL homeostasis, including an age-dependent decline in SL levels that correlates with reduced branching inhibition in maturing plants.^[41] Additionally, feedback from auxin transport inhibitors, such as N-1-naphthylphthalamic acid, indirectly influences SL by disrupting polar auxin flow, which in turn alters SL-mediated bud regulation through reciprocal signaling loops.^[42] Species-specific variations highlight adaptive SL flux, with higher biosynthesis rates observed in mycotrophic plants reliant on mycorrhizal symbioses compared to non-mycotrophic ones, facilitating efficient nutrient foraging. Recent 2025 studies on barley mutants with SL insensitivity, such as the hvd14 line, reveal disrupted hormonal homeostasis, including altered auxin and jasmonate balances, underscoring SL's role in maintaining developmental equilibrium across species.^[43]

Perception and Signaling

Molecular Receptors

The primary molecular receptor for strigolactones (SLs) in plants is the α/β-hydrolase protein DWARF14 (D14), also known as DAD2 in petunia and DWARF14 in rice, which directly perceives SLs by binding and hydrolyzing them.^[44] Upon SL binding, D14 undergoes covalent modification through hydrolysis of the butenolide D-ring, forming a covalent intermediate that induces a conformational change from an open to a closed state, enabling interaction with downstream signaling components.^[19] This hydrolysis is essential for SL perception, as D14 acts as a dual-function enzyme-receptor that both senses and deactivates the hormone.^[44] Crystal structures of the D14-SL complex, first resolved in 2013, reveal a deep hydrophobic binding pocket in the α/β-hydrolase fold that accommodates the tricyclic ABC-ring system of canonical SLs, with specificity conferred by hydrogen bonding to the butenolide ring and surrounding residues like Ser97 and His246.^[45] For instance, the synthetic analog GR24 binds with high affinity (Kd ~1-10 μM) to this pocket, mimicking natural SLs such as strigol, while non-canonical SLs lacking the full ABC scaffold exhibit lower or variable affinities, highlighting D14's preference for structurally conserved features.^[9] SL perception by D14 recruits accessory proteins to initiate signaling, including the F-box protein MAX2 (D3 in rice) and the ASK1 adaptor, which form an SCF ubiquitin ligase complex that targets transcriptional repressors for degradation.^[46] In rice, the repressor D53, and its orthologs SMXL6, SMXL7, and SMXL8 in Arabidopsis, interact with the D14-MAX2 complex upon SL-induced conformational change, leading to their polyubiquitination and proteasomal degradation to derepress target genes.^[46] D14 orthologs are highly conserved across angiosperms, sharing over 70% sequence identity and retaining the core catalytic triad (Ser-His-Asp) essential for SL hydrolysis, with unambiguous presence in seed plants but absence in non-vascular plants like mosses. A 2025 ancestral reconstruction confirms D14 evolved at the base of seed plants via duplication of the karrikin receptor KAI2, with conserved substrate specificity.^[47]^[48]

Signal Transduction Pathways

Strigolactones (SLs) initiate intracellular signaling primarily through interaction with the α/β-hydrolase receptor DWARF14 (D14), which undergoes a conformational change upon binding. This activated D14 recruits the F-box protein DWARF3 (D3)/MORE AXILLARY GROWTH 2 (MAX2) and the transcriptional repressor DWARF53 (D53) or its orthologs SMXL6–8, forming a complex that facilitates ubiquitination of D53/SMXLs by the SCF^D3/MAX2 E3 ligase.^[49] The polyubiquitinated D53/SMXLs are then targeted for degradation by the 26S proteasome, relieving transcriptional repression on downstream targets. This negative regulatory model—where SLs act by removing repressors rather than directly activating transcription factors—underpins the core SL signaling cascade across angiosperms.^[50] Degradation of D53/SMXLs derepresses key target genes, such as the shoot branching inhibitor BRANCHED1 (BRC1)/TEOSINTE BRANCHED1 (TB1), which encodes a TCP transcription factor that suppresses axillary bud outgrowth.^[51] Promoters of SL-responsive genes, including BRC1, contain enriched motifs such as TCP, MYB, and basic pentacysteine (BPC) elements, often within differentially accessible chromatin regions identified via ATAC-seq, enabling rapid transcriptional activation post-D53 degradation.^[52] In rice, additional targets like IDEAL PLANT ARCHITECTURE1 (IPA1) further integrate SL responses with tillering control.^[53] SL signaling incorporates feedback loops to fine-tune its activity. High SL levels promote D53 degradation, which indirectly represses SL biosynthesis genes (e.g., CCD7/MAX3 and D27) through derepression of intermediary regulators, establishing negative feedback that limits excessive accumulation. Additionally, IPA1 binds the D53 promoter to upregulate its expression, forming a coherent feed-forward loop that buffers SL responsiveness.^[53] In rice, D53 stability is further modulated by phosphorylation from glycogen synthase kinase 3 (GSK3)-like kinases, which antagonize brassinosteroid (BR) signaling by stabilizing D53–OsBZR1 complexes and enhancing repression of tillering genes like FC1.^[54] Recent studies highlight SL involvement in leaf senescence, where D14-mediated signaling promotes degradation of repressors that regulate WRKY transcription factors, thereby activating salicylic acid-dependent senescence programs in response to nutrient deficiencies or darkness.^[55]

Physiological Functions in Plants

Inhibition of Shoot Branching

Strigolactones (SLs) play a central role in maintaining shoot apical dominance by inhibiting the outgrowth of axillary buds, thereby shaping overall plant architecture and optimizing resource allocation to the main stem. This inhibition is particularly evident in response to environmental stresses, where elevated SL levels suppress lateral branching to enhance survival and growth efficiency. The process integrates SL signaling with auxin transport dynamics, ensuring that bud dormancy is maintained until favorable conditions arise. The primary mechanism underlying SL-mediated inhibition involves the downregulation of auxin transport to axillary buds, achieved through rapid depletion of the auxin efflux carrier PIN1 from the plasma membrane in vascular tissues. This reduces auxin canalization necessary for bud outgrowth, creating a feedback loop where SLs counteract auxin accumulation in buds and promote competition among potential branches. In SL signaling mutants such as max2 in Arabidopsis thaliana, this regulatory control is lost, resulting in excessive branching phenotypes with significantly more axillary shoots compared to wild-type plants. Similarly, application of the synthetic SL analog GR24 to rice (Oryza sativa) effectively suppresses tiller bud outgrowth in SL biosynthesis mutants like d10, restoring normal architecture and demonstrating the direct inhibitory action of SLs. This mechanism operates through brief crosstalk with auxin pathways, where SL perception via D14 and MAX2 components modulates auxin responsiveness without altering core biosynthesis.^[56]^[42] SL levels are dynamically regulated by environmental factors, notably nutrient deficiencies, which enhance SL production and exudation to further inhibit branching and redirect resources toward primary growth. For instance, nitrogen or phosphate limitation increases SL accumulation in shoot bases, leading to reduced lateral bud activation and improved main stem prioritization, as observed in Arabidopsis under low-nitrogen conditions. Quantitatively, higher endogenous SL concentrations correlate inversely with branch number across species; in tomato (Solanum lycopersicum) SL biosynthesis mutants, branch counts rise by up to sevenfold alongside decreased SL levels, underscoring this relationship. Recent 2025 studies in barley (Hordeum vulgare) on SL-insensitive hvd14 mutants reveal nearly double the tiller number (27 versus 14 in wild-type) at maturity, highlighting how reduced SL sensitivity can enhance branching and potentially elevate yield potential through increased reproductive sites, though balanced against grain size trade-offs.^[57]^[43] This inhibitory function is active throughout key developmental stages, from post-germination seedling establishment through vegetative expansion to the onset of reproduction, allowing plants to adapt architecture dynamically to internal cues and external challenges. During these phases, SLs ensure that branching responds to nutrient availability and light competition, preventing wasteful proliferation under stress while permitting outgrowth when resources abound.^[58]

Regulation of Root and Secondary Growth

Strigolactones (SLs) play a crucial role in modulating root system architecture, particularly under nutrient-limited conditions. Under low phosphate availability, SL levels increase to promote lateral root formation, enhancing the plant's ability to explore soil for nutrients.^[59] This promotion is evident in species like rapeseed, where exogenous SL application stimulates lateral root growth by reducing endogenous auxin levels, thereby fine-tuning root branching for efficient phosphate acquisition.^[60] Conversely, SLs inhibit primary root elongation through the degradation of the transcriptional repressor D53 (or its orthologs like D53-like SMXLs), which suppresses meristem activity and limits excessive downward growth in favor of lateral expansion; effects on root elongation vary by species and conditions, with inhibition of primary root length in Arabidopsis but promotion of crown roots in rice.^[49]^[61] In rice, for instance, SL signaling via D53 modulates root elongation in response to nitrate supply, highlighting its conserved role across species.^[62] In secondary growth, SLs act in an auxin-mediated manner to enhance cambial proliferation, contributing to vascular tissue development in woody plants. SL signaling is essential for auxin-dependent stimulation of the vascular cambium, a stem cell niche responsible for radial growth in stems and roots.^[63] Studies in Arabidopsis SL biosynthetic and signaling mutants demonstrate reduced cambium activity, underscoring SLs' direct influence on this process.^[64] In trees like poplar, mutants defective in SL perception exhibit altered woody architecture, with diminished secondary thickening and impaired higher-order branching, indicating SLs' importance for perennial growth patterns.^[65] The underlying mechanism involves a dynamic feedback loop between SLs and auxin, where SLs upregulate indole-3-acetic acid (IAA) transport in roots via modulation of PIN-FORMED (PIN) efflux carriers.^[66] This interaction allows SLs to fine-tune auxin distribution, promoting localized accumulation that drives lateral root initiation while restraining primary root extension.^[42] In roots, SLs specifically enhance auxin canalization, ensuring efficient transport and feedback regulation of developmental cues.^[67] Under drought stress, SL biosynthesis is upregulated, leading to finer root systems that improve water uptake efficiency. This adaptation involves increased SL-mediated root hair density and reduced primary root length, optimizing the root-to-shoot ratio for stress tolerance.^[68] Recent 2025 studies further reveal SLs' role in woody architecture and drought resilience in species like poplar.^[64] For example, application of the synthetic SL analog GR24 to Arabidopsis roots increases root hair density, enhancing surface area for water absorption without altering overall biomass.^[69]

Other Developmental Roles

Strigolactones (SLs) play a significant role in promoting leaf senescence in plants, particularly through interactions with abscisic acid (ABA) signaling via the DWARF14 (D14) receptor. In Arabidopsis, SL biosynthesis mutants exhibit delayed leaf senescence during ethylene-induced conditions, indicating that SLs actively accelerate the aging process to facilitate nutrient remobilization under stress.^[70] This promotion occurs partly through SL-induced ABA biosynthesis, which enhances ABA-dependent transcriptional responses and senescence-associated gene expression, including under drought conditions.^[71] Mutants defective in the D14 receptor, such as d14, display delayed ABA-induced leaf senescence, underscoring the pathway's reliance on SL perception for timely aging.^[72] SLs also influence seed germination and dormancy, promoting embryo growth in certain species while exhibiting crosstalk with gibberellins (GAs). In Arabidopsis, SLs alleviate thermoinhibition to stimulate seed germination by modulating GA signaling pathways that drive embryo expansion and radicle emergence.^[73] This interaction involves SLs acting downstream or in concert with GAs to fine-tune dormancy release, as evidenced by SLs modulating GA biosynthesis genes to counteract ABA-mediated inhibition during germination.^[74] For instance, in thermoinhibited seeds, exogenous SL application enhances germination rates by promoting GA-dependent embryo growth without directly affecting parasitic weed responses.^[75] Regarding flowering and fruiting, SLs exert indirect regulatory effects, often delaying flowering under stress conditions in crops like rice to prioritize vegetative survival. In rice, SL signaling through the D14 receptor represses FLOWERING LOCUS T (FT) expression, thereby postponing the transition to reproductive phases during abiotic stresses such as drought.^[76] This delay maintains a prolonged vegetative phase, as seen in overexpression lines of SL-related genes that extend vegetative growth and reduce sensitivity to flowering cues under environmental pressure.^[41] SLs further contribute to stress tolerance, notably enhancing drought resistance through ABA-mediated stomatal closure. Exogenous SL application induces rapid stomatal closure in tomato and apple, reducing transpiration and improving water use efficiency by upregulating ABA-responsive genes.^[77] Recent 2025 research highlights SLs' role in modulating vessel formation to optimize water usage under drought in Arabidopsis, with implications for stress resilience in perennial species like poplar.^[78]^[64]

Roles in Symbiotic and Parasitic Interactions

Promotion of Arbuscular Mycorrhizal Symbiosis

Strigolactones (SLs) are exuded from plant roots into the rhizosphere, particularly under conditions of low nutrient availability such as phosphate deficiency, serving as signaling molecules to initiate interactions with arbuscular mycorrhizal fungi (AMF). This exudation promotes the presymbiotic growth of AMF by stimulating extensive hyphal branching, which facilitates the fungus's approach to the host root. For instance, in species like Rhizophagus irregularis, SL concentrations as low as approximately 10^{-9} M trigger rapid and pronounced hyphal branching, enhancing the probability of successful root colonization.^[79] Upon release into the soil, SLs are perceived by the AMF, activating metabolic responses that support presymbiotic development and subsequent arbuscule formation within root cortical cells. In fungi such as Gigaspora rosea, SLs rapidly stimulate mitochondrial activity, leading to increased respiration, ATP production, and cell proliferation, which collectively drive hyphal elongation and branching toward the host. Although specific SL receptors in AMF remain under investigation, studies indicate involvement of putative G-protein-coupled receptors that initiate these downstream effects, promoting the transition from saprotrophic to symbiotic growth.^[80]^[81] This symbiotic association provides significant benefits to the host plant, primarily through enhanced nutrient acquisition, especially phosphorus, which is efficiently transported via the fungal hyphae into the root system. Plants deficient in SL biosynthesis, such as the max3 mutant in Arabidopsis thaliana that lacks the carotenoid cleavage dioxygenase CCD7, exhibit substantially reduced AMF colonization rates and impaired phosphorus uptake compared to wild-type plants, underscoring SLs' critical role in symbiosis establishment.^[82] SL specificity in fungal interactions highlights structural preferences, with canonical SLs like orobanchol showing higher efficacy in stimulating AMF hyphal branching relative to non-canonical forms, whereas strigol exhibits greater potency in parasitic contexts. Recent investigations into SL concentration gradients in soil have revealed how these molecules form localized hotspots near roots under nutrient stress, optimizing AMF recruitment while minimizing diffusion losses in heterogeneous environments.^[83]^[84] In plants, SL signaling integrates with the common symbiosis pathway to regulate intracellular accommodation of the fungus, where key components like the leucine-rich repeat receptor-like kinase SYMRK play essential roles downstream of initial SL perception, enabling arbuscule maturation and nutrient exchange.^[85]^[86]

Stimulation of Parasitic Weed Germination

Strigolactones (SLs) exuded from host plant roots serve as key signaling molecules that trigger seed germination in root parasitic weeds such as Striga and Orobanche species. These compounds, including natural variants like 5-deoxystrigol produced by cereals such as sorghum and maize, are released into the rhizosphere where they are perceived by dormant parasite seeds, initiating the transition from conditioning to germination.^[87]^[88] Upon germination, the parasitic seedlings develop a haustorium—a specialized attachment organ—that penetrates the host root to extract water and nutrients, often leading to severe crop damage.^[89] This process exploits the host's SL signaling, originally evolved for symbiotic interactions, but hijacked by parasites for their obligate parasitic lifestyle.^[87] Parasitic weeds have evolved exceptional sensitivity to SLs, enabling detection at trace concentrations that reflect host presence. For instance, Striga hermonthica seeds exhibit up to 93% germination in response to strigol at concentrations as low as 10^{-8} M, with some synthetic SL analogs achieving responses at even lower levels, such as 10^{-17} M.^[89]^[87] This high specificity arises from the parasites' adaptation to recognize the conserved D-ring structure of SLs, with variations in the ABC moieties influencing host-parasite compatibility; for example, Striga gesnerioides preferentially responds to 4-hydroxy-substituted GR24 analogs.^[89] Such sensitivity ensures that parasites germinate only near potential hosts, minimizing energy waste in nutrient-poor soils.^[90] Host plants can counter this interaction through genetic variations that reduce SL exudation, conferring resistance to infestation. In sorghum, mutations in the LOW GERMINATION STIMULANT 1 (lgs1) gene decrease levels of 5-deoxystrigol in root exudates while increasing less active orobanchol, resulting in up to 90% reduced Striga germination and attachment compared to wild-type plants.^[88] Similarly, maize lines with altered SL profiles show enhanced resistance by limiting stimulant availability.^[91] To exploit this vulnerability, agricultural strategies employ synthetic SL analogs like GR24, which induce "suicide germination" in parasite seeds without a nearby host, depleting soil seed banks by over 95% in field applications.^[89] This host-parasite dynamic represents an evolutionary arms race, where plants diversify SL profiles to evade detection while parasites enhance receptor sensitivity and potentially produce inhibitors to manipulate host responses. Parasitic weeds like Striga and Orobanche have co-opted D14-like α/β-hydrolase receptors, such as ShHTL7 in Striga hermonthica, which bind SLs via a covalent nucleophilic addition to the butenolide ring, hydrolyzing the molecule and activating downstream signaling for germination.^[87]^[90] Recent studies highlight how non-host plants may employ SL mimicry or reduced exudation as a defense, with 2025 research demonstrating that manipulating SL transporters in crops like tomato can limit parasite attachment without compromising yield.00018-9.pdf)^[92] The global impact of SL-induced parasitic infestations is profound, affecting over 50 million hectares of arable land primarily in sub-Saharan Africa and causing annual yield losses estimated at $7 billion USD, particularly in staple crops like maize and sorghum that support over 100 million smallholder farmers.^[93]^[94] These interactions underscore the dual-edged nature of SL signaling, where a beneficial cue for symbiosis becomes a liability in agroecosystems dominated by parasitic weeds.^[95]

Hormone Crosstalk and Ecological Implications

Interactions with Other Phytohormones

Strigolactones (SLs) engage in extensive crosstalk with other phytohormones, integrating signaling pathways to fine-tune plant architecture, stress responses, and developmental processes. This integration positions SLs as key regulators in hormonal networks, where they modulate auxin transport, synergize with abscisic acid (ABA) under abiotic stress, synergize with gibberellin (GA) effects on branching, and interact with ethylene, cytokinin, and jasmonate to influence growth and defense.^[96] SLs and auxin exhibit bidirectional crosstalk that controls shoot branching and root development. In shoots, SLs inhibit auxin maxima in axillary buds by targeting PIN1-dependent auxin transport, reducing polarized PIN1 localization and vascular reconnection to suppress bud outgrowth in species like pea and Arabidopsis. This mechanism involves SLs attenuating auxin feedback on PIN endocytosis, thereby limiting auxin canalization from buds to the main stem. Reciprocally, auxin upregulates SL biosynthetic genes such as MAX3 and MAX4 in Arabidopsis shoots, forming a dynamic feedback loop where elevated auxin in SL-deficient mutants boosts SL production to restore balance. In roots, auxin enhances SL responses, with additive effects on processes like root hair elongation, while SL signaling modulates auxin distribution for secondary growth. SLs synergize with ABA to enhance drought tolerance, sharing components in stress signaling pathways. Under drought, ABA induces SL biosynthesis in roots, as seen in rice where mild drought elevates SL levels 10-fold alongside ABA-responsive gene expression in shoots. This interaction converges on shared regulators, such as the D14 receptor and ABI5 transcription factor, where SL perception via D14 activates ABI5 to promote proline accumulation and stomatal closure in apple. In the MsABI5-MsD14 module, ABA and SLs co-regulate downstream targets like NAC transcription factors, amplifying abiotic stress responses without direct evidence of reciprocal induction in all contexts.^[97] SLs and GA synergistically regulate branching in rice through the SLR1-OsMADS23-D14 complex. GA degrades the DELLA repressor SLR1 to relieve repression on developmental genes, while SLs via D14 destabilize the OsMADS23-SLR1 complex to suppress tillering and inhibit bud outgrowth. GA also negatively regulates SL biosynthesis by downregulating genes like D10 and D27 via GID1-DELLA signaling, yet SL-mediated branching inhibition requires intact GA signaling, as GA deficiency impairs SL effects on tiller suppression. Jasmonate (JA) interacts with SLs in defense responses, where SL deficiency elevates JA levels up to 30-fold, enhancing flavonoid phytoalexin production and resistance to pathogens like rice blast fungus, suggesting JA amplifies SL-dependent immunity.^[98] SLs modulate ethylene and cytokinin signaling, often antagonistically, to control developmental transitions. In root hair elongation, SLs require ethylene biosynthesis to promote growth, with both hormones acting additively with auxin through MAX2-dependent pathways in Arabidopsis. Cytokinins promote bud outgrowth in opposition to SLs, which reduce cytokinin content in rice tiller buds by upregulating CKX genes for cytokinin degradation, thereby suppressing OsRR expression and tillering. Recent 2025 studies highlight SL-auxin balance in perennials, where SL insensitivity in barley mutants disrupts hormonal homeostasis, lowering auxin and ABA while elevating cytokinins to alter architecture. Overall, SLs serve as hubs in phytohormone homeostasis; for instance, SL depletion in the dad1 mutant of tomato leads to hyperbranching that mimics auxin overproduction phenotypes, reversible by exogenous SL application, underscoring SLs' role in maintaining integrated hormonal equilibria.

Evolutionary and Ecological Significance

Strigolactones (SLs) represent an ancient signaling pathway with origins tracing back to charophyte algae, where precursors and responses to SL-like molecules facilitated early adaptations to terrestrial environments. Evidence from phylogenetic analyses indicates that SL biosynthesis and perception components, such as KAI2-like proteins, emerged prior to the divergence of streptophytes, enabling primitive functions in rhizoid development and nutrient acquisition. In seed plants, the evolution of canonical SL biosynthesis involved key enzymatic transitions, notably the derivation of CYP722C from CYP722A, which shifted oxidation sites to produce rhizosphere-active SLs that enhance interorganismal signaling with microbes and parasites. This biosynthetic innovation, detailed in recent synthetic biology studies, underscores how SLs transitioned from internal hormonal roles to external ecological signals during seed plant diversification.^[99] Conservation of SL signaling components is evident across land plants, with orthologs of MAX2 present in bryophytes like Physcomitrella patens, supporting promiscuous upstream interactions for developmental regulation. In contrast, canonical D14 orthologs are restricted to seed plants, suggesting neo-functionalization of receptors post-gymnosperm emergence. Following the angiosperm radiation approximately 140 million years ago, SL structural diversity expanded dramatically, with over 25 variants identified, driven by co-evolutionary pressures from root parasites such as Striga and Orobanche. This diversification allowed hosts to modulate SL profiles—e.g., increasing orobanchol relative to 5-deoxystrigol in sorghum—to maintain mycorrhizal benefits while reducing parasite germination, thereby evading infestation without compromising symbiosis. Ecologically, SLs play a pivotal role in nutrient foraging under phosphorus-poor soils by promoting hyphal branching in arbuscular mycorrhizal fungi (AMF), enhancing host mineral uptake and stress tolerance. This symbiotic facilitation influences plant community structure, as SL exudation gradients shape microbial recruitment and competitive hierarchies, favoring species with efficient AMF associations in nutrient-limited ecosystems. Under climate warming, SL exudation often increases in response to abiotic stresses like elevated temperatures and drought, altering root-associated microbial diversity by boosting beneficial AMF while potentially amplifying parasite risks through heightened germination cues—a trade-off that could exacerbate weed pressures in warming agroecosystems. SL biodiversity is notably higher in tropical crops, such as sorghum and maize, where diverse SL profiles (e.g., sorgolactone and ent-2'-epi-5-deoxystrigol) reflect adaptations to intense parasite and nutrient challenges in arid tropics. This structural variation contributes to invasive species dynamics, as high-SL-exuding invasives rapidly establish AMF symbioses, outcompeting natives by altering rhizosphere microbiomes and resource allocation in novel habitats.

Agricultural Applications

Strategies for Weed Control

Breeding efforts have focused on developing low-strigolactone (SL) crop varieties to reduce the stimulation of Striga seed germination, thereby conferring resistance to this parasitic weed prevalent in sub-Saharan Africa. In maize, alterations in SL biosynthesis pathways, such as mutations in cytochrome P450 enzymes like ZmCYP706C37, have been shown to decrease SL exudation and Striga hermonthica emergence in field conditions, enabling the deployment of resistant lines in African smallholder farming systems.^[91] Similarly, in sorghum, the low germination stimulant 1 (lgs1) mutant, which disrupts a MAX1-like gene in SL biosynthesis, reduces Striga emergence by altering the composition of root-exuded SLs, with field trials demonstrating yield improvements under infestation.^[88] For rice, natural deletions of two MAX1 orthologs (OsMAX1-3 and OsMAX1-7) in varieties like the indica cultivar Bala lead to low SL production and reduced Striga infection, informing breeding programs that incorporate such traits into elite lines derived from IR64, a widely grown Asian rice variety adapted for African contexts.^[100] Chemical mimics of SLs, such as the synthetic analog GR24 and the natural compound dehydrocostus lactone (DCL), are applied to induce suicidal germination in Striga seeds, prompting them to sprout in the absence of a host and deplete the soil seedbank. GR24, when formulated in granular or emulsion concentrates and applied as a soil treatment or via trap crops like cowpea, has achieved significant reductions in Striga emergence in sorghum and maize fields, promoting the use of non-host trap crops to enhance efficacy without harming the main crop.^[101] DCL, isolated from sunflower roots, stimulates germination of parasitic weeds like Orobanche cumana and shows promise for suicidal strategies against related parasites, with recent formulations improving soil persistence for field application.^[102] RNA interference (RNAi) strategies targeting the parasite's D14 receptor, a key SL perception component in Striga hermonthica, aim to desensitize the weed to host signals and prevent attachment. Host-induced gene silencing (HIGS) via transgenic crops expressing RNAi constructs against ShD14 has reduced Striga germination sensitivity in greenhouse assays, with constructs delivered through root exudates silencing the parasite gene post-germination.^[103] Field trials in sorghum, incorporating HIGS lines combined with low-SL backgrounds, have demonstrated improved grain yields in Striga-infested plots, highlighting the potential for durable resistance without broad off-target impacts on host physiology. Integrated management approaches combine low-SL breeding with herbicides and biological agents to achieve synergistic Striga control. For instance, seed dressing with low-dose imidazolinone herbicides on resistant maize varieties provides post-emergence kill of attached Striga while minimizing crop injury, resulting in three- to four-fold yield increases in sub-Saharan trials.^[104] Recent advances include soil applications of SL-degrading bacteria from suppressive soils, which accelerate GR24 breakdown and offer a sustainable, non-chemical option for seedbank depletion when integrated with crop rotation. Despite these strategies, challenges persist, including off-target effects on arbuscular mycorrhizal fungi (AMF), where SL analogs or inhibitors like methyl phenlactonoates can suppress spore germination and root colonization, potentially reducing crop nutrient uptake in phosphorus-poor African soils.^[105] Overall, low-SL and integrated approaches show varying efficacy in maize and sorghum but face limitations in scaling due to seed availability and environmental variability.^[106]

Enhancement of Crop Symbiosis and Stress Tolerance

Strigolactones (SLs) play a pivotal role in enhancing arbuscular mycorrhizal (AM) symbiosis in crops by acting as rhizospheric signals that stimulate fungal hyphal branching and colonization. Exuded from plant roots at concentrations as low as 10^{-13} M, SLs rapidly activate mitogen-activated protein kinase signaling in AM fungi such as Gigaspora rosea, increasing mitochondrial density by up to 32% within hours and boosting fungal respiration for pre-symbiotic growth. This enhanced fungal development facilitates greater root-fungus contact, promoting AM colonization that improves phosphorus and nitrogen uptake in crops like tomato and rice, particularly under nutrient-deficient conditions where SL production can increase up to 30-fold.^[107] In agricultural contexts, this symbiosis mitigates nutrient stress, leading to improved crop yield and resilience, as demonstrated in alfalfa where SL-mediated AM interactions enhance overall plant vigor.^[107] Beyond symbiosis, SLs directly contribute to abiotic stress tolerance in crops by modulating physiological responses and hormone crosstalk. Under drought, SLs increase abscisic acid (ABA) sensitivity, promoting stomatal closure and reducing water loss; for instance, exogenous SL application (e.g., GR24) elevates wild-type Arabidopsis survival rates to 100% under severe dehydration, compared to 29% without treatment.^[108] SL-deficient mutants exhibit hypersensitivity due to higher stomatal density and slower ABA-mediated closure, highlighting SL's regulatory role.^[108] Similarly, in salinity stress, SLs bolster ABA responses in crops like tomato, enhancing root architecture and antioxidant activity to counteract ionic toxicity.^[107] These mechanisms extend to other stresses, including cadmium toxicity, where SLs reduce oxidative damage via upregulated antioxidant systems. In crop improvement, manipulating SL biosynthesis or perception—through genetic engineering or exogenous applications—offers strategies to amplify symbiosis and stress tolerance. For example, elevating SL levels in rice and maize promotes AM associations that buffer drought effects by optimizing nutrient acquisition and photosynthetic efficiency. SLs also interact with auxins and cytokinins to refine root morphology under low-phosphate conditions, further supporting symbiotic benefits.^[107] Such approaches hold promise for developing resilient varieties, as SL modulation has been shown to safeguard photosynthetic performance and gene expression under combined nutrient and water deficits. As of 2025, new molecular markers for lgs1 mutations in sorghum enhance breeding for Striga resistance while maintaining symbiotic benefits.^[109]

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Strigolactone Regulates Leaf Senescence in Concert with Ethylene ...
Leaf senescence is regulated by various factors, such as darkness, stress, aging, and phytohormones. Strigolactone is a recently identified phytohormone, and it ...
[68]
Strigolactones positively regulate abscisic acid-dependent heat and ...
Nov 1, 2021 · Arbuscular mycorrhizal symbiosis induces strigolactone biosynthesis under drought and improves drought tolerance in lettuce and tomato.
[69]
Comparative functional analyses of DWARF14 and KARRIKIN ...
On the other hand, D14 had a lesser role in the maintenance of cell membrane integrity, leaf cuticle structure and ABA-induced leaf senescence, but a greater ...
[70]
Thermoinhibition Uncovers a Role for Strigolactones in Arabidopsis ...
Strigolactones are host factors that stimulate seed germination of parasitic plant species such as Striga and Orobanche. This hormone is also important in ...
[71]
Regulation of Strigolactone Biosynthesis by Gibberellin Signaling
Apr 12, 2017 · Strigolactones (SLs) are a class of plant hormones that regulate diverse physiological processes, including shoot branching and root development ...
[72]
HY5 is involved in strigolactone-dependent seed germination in ...
SLs also act as an environmental signal to stimulate seed germination of parasitic plant species of genera Striga and Orobanche.
[73]
The strigolactone receptor DWARF14 regulates flowering time in ...
This results in reduced FT transcription and delayed flowering. In summary, AtD14 perception of SL enables the transcription factor TOE1 to repress flowering, ...
[74]
Strigolactones as master regulators of plant responses to abiotic ...
Strigolactones (SLs) are an emerging class of plant hormones that play important roles in plant growth, development, and response to environmental stresses.
[75]
Strigolactones optimise plant water usage by modulating vessel ...
Apr 28, 2025 · We reveal that the strigolactone (SL) signalling pathway negatively regulates vessel element formation, impacting plant water usage.
[76]
[PDF] Perception and responses to strigolactones in arbuscular ...
May 12, 2023 · Stereochemical Assignment of Strigolactone Analogues Confirms Their Selective Biological Activity. J Nat Prod. 78:2624–2633. https://doi.org ...<|separator|>
[77]
Strigolactones Stimulate Arbuscular Mycorrhizal Fungi by Activating ...
Jun 27, 2006 · Strigolactones strongly and rapidly stimulated cell proliferation of the AM fungus Gigaspora rosea at concentrations as low as 10 −13 M. This ...
[78]
Strigolactones stimulate arbuscular mycorrhizal fungi by ... - PubMed
Strigolactones strongly and rapidly stimulated cell proliferation of the AM fungus Gigaspora rosea at concentrations as low as 10(-13) M. This effect was not ...
[79]
A Dual Role of Strigolactones in Phosphate Acquisition and ... - NIH
Apr 9, 2013 · For example, the length of primary roots of Arabidopsis SL-deficient and -insensitive mutants are shorter due to a reduction in meristem cell ...
[80]
The potential of strigolactones to shift competitive dynamics among ...
Oct 17, 2024 · Strigolactones are phytohormones that influence arbuscular mycorrhizal fungal (AMF) spore germination, pre-symbiotic hyphal branching, and metabolic rates.Missing: GROR1 | Show results with:GROR1
[81]
The significant effects of Strigolactones on plant growth and microbe ...
May 6, 2025 · Strigolactones (SLs) function as both hormones within plants and signaling molecules in the rhizosphere, interacting with mycorrhizal fungi ...<|control11|><|separator|>
[82]
plants, fungi, and bacteria in the arbuscular mycorrhizal symbiosis
Strigolactones (SLs) are signal molecules that communicate with soil organisms, including AMF and bacteria, in the rhizosphere.Introduction · Strigolactones: their impact on... · The impact of strigolactones...
[83]
Conservation of symbiotic signaling since the most recent common ...
Here, we found that intracellular colonization by AM fungi induces a transcriptional reporter of the common symbiosis pathway, well-described in angiosperms, in ...
[84]
Probing strigolactone perception mechanisms with rationally ...
Jul 9, 2022 · SLs all have a conserved enol-ether butenolide functional group ... That is, the reaction may be initiated through the nucleophilic attack ...
[85]
Mutation in sorghum LOW GERMINATION STIMULANT 1 ... - PNAS
Apr 10, 2017 · We report on identification of a gene regulating Striga resistance in sorghum and the associated change in strigolactone chemistry.
[86]
https://www.pnas.org/doi/10.1073/pnas.2408539121
[87]
Structure-function analysis identifies highly sensitive strigolactone ...
Oct 9, 2015 · We characterized the function of 11 strigolactone receptors from the parasitic plant Striga hermonthica using chemical and structural biology.<|separator|>
[88]
Maize resistance to witchweed through changes in strigolactone ...
Jan 5, 2023 · Parasitic witchweed (Striga) reduces the yield of maize grown in infected fields. Strigolactones from maize roots encourage Striga germination.
[89]
Phytoparasite avoidance: Manipulation of strigolactone exudation ...
May 23, 2025 · Interestingly, the interaction between host plants and parasitic weeds resembles an “arms race”, with each party evolving novel strategies ...
[90]
Striga hermonthica: A highly destructive pathogen in maize production
It has been calculated that over 50 million hectares of tillable soils under cereals cultivation, including maize, have been infested by Striga spp. (Dafaallah, ...
[91]
Testcross performance of Striga-resistant maize inbred lines and ...
Apr 5, 2024 · Striga hermonthica has been identified in 32 countries, infesting over 50 million ha of arable land and causing an estimated 7 billion US$ yield ...
[92]
Cracking the enigma: understanding strigolactone signalling in ... - NIH
In this review, we examine the multi-faceted roles of strigolactones as signals in the rhizosphere, and try to understand why plants exude so many different ...
[93]
Error | Proceedings of the National Academy of Sciences
**Summary:**
[94]
Natural variation of rice strigolactone biosynthesis is associated with ...
In shoots, SLs inhibit tillering; in roots, SLs influence root and root ... Strigolactones affect lateral root formation and root-hair elongation in Arabidopsis.
[95]
A New Formulation for Strigolactone Suicidal Germination Agents ...
Mar 18, 2022 · The germination of Striga seeds can be triggered in the bare soil by direct application of synthetic germination stimulants [24,25]. This ...Missing: dehydrocostus | Show results with:dehydrocostus<|separator|>
[96]
dynamics of strigolactone perception in Striga hermonthica
A working hypothesis for the dynamics of strigolactone perception in the parasitic plant Striga hermonthica is proposed.
[97]
Trenchant microbiological-based approach for the control of Striga
Apr 5, 2023 · Also, there is evidence of fungal degradation by Strigolactone, which may result to decrease in Striga germination and the release of root ...
[98]
Current progress in Striga management - PMC - PubMed Central - NIH
Maize has surpassed the traditional cereals in SSA, with the highest cultivation area of about 39 million ha in 2018 (FAOSTAT, 2018). Considering the ...
[99]
Development and application of a bioassay for assessing the ...
Mar 17, 2024 · Using our bioassay, it will be possible to pinpoint the assemblies of soil microbes that play a role in the degradation of strigolactones.
[100]
Effect of the strigolactone analogs methyl phenlactonoates on spore ...
Our results show that MP1 and MP3 inhibit AMF spore germination, but promote the intra-radical root colonization, both more efficiently than GR24.1. Introduction · 2. Materials And Methods · 3. Results
[101]
Current progress in Striga management | Plant Physiology
Feb 5, 2021 · In this paper, we provide an update on the recent progress and the approaches used in Striga management, and highlight emerging opportunities for developing ...
[102]
https://www.sciencedirect.com/science/article/abs/pii/S0031942211000665
[103]
https://academic.oup.com/jxb/article/69/9/2281/4883187