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Haemonchus contortus

Haemonchus contortus, commonly known as the barber's pole worm due to its distinctive twisted appearance, is a hematophagous parasitic nematode belonging to the family Haemonchidae that primarily infects the abomasum of small ruminants such as sheep and goats.^[1] This blood-feeding worm is one of the most pathogenic gastrointestinal nematodes worldwide, causing haemonchosis, a disease characterized by severe anemia, weight loss, edema, and high mortality rates in infected animals.^[2] Its direct life cycle involves eggs passed in host feces that hatch into larvae (L1 to L3 stages) in warm, moist environments, with the infective third-stage larvae (L3) being ingested by grazing ruminants to develop into adults in the abomasum, where females can produce up to 10,000 eggs per day.^[2] Native to tropical and subtropical regions but now distributed globally, H. contortus poses significant economic challenges to the livestock industry, leading to reduced productivity, increased veterinary costs, and the need for integrated control strategies including anthelmintics, pasture management, and emerging vaccines.^[2]

Overview

Etymology and discovery

The genus name Haemonchus derives from the Greek words haima (blood) and onchos (barb or hook), alluding to the nematode's blood-feeding behavior and the barbed, spear-like structure of its mouthparts used for piercing host tissues. The species epithet contortus originates from the Latin term meaning "twisted" or "coiled," reflecting the worm's tightly coiled appearance in preserved specimens. These names highlight key morphological and ecological features that distinguish the parasite within the Trichostrongylidae family.^[3] Haemonchus contortus was first described in 1803 by the German parasitologist Karl Asmund Rudolphi, who classified it as Strongylus contortus based on specimens recovered from the abomasum of sheep. Rudolphi's description, published in his comprehensive work on entozoa, marked the initial scientific recognition of the nematode as a distinct entity among ruminant parasites, though its pathogenic potential was not fully appreciated at the time. In 1898, American nematologist Nathan Augustus Cobb reclassified the species into the newly established genus Haemonchus, emphasizing diagnostic traits such as the male bursa morphology and the female's vulval structure. This taxonomic revision solidified its placement within the strongylid nematodes and facilitated further studies on its biology.^[4]^[5] Early observations of H. contortus in the 19th century were primarily documented in Europe, where parasitologists noted its presence in the abomasa of domestic sheep during post-mortem examinations, often associating it with anemia but not yet linking it to widespread epizootics. By the early 20th century, the parasite gained recognition as a major pathogen in regions outside Europe, particularly in Australia and South Africa, following outbreaks in imported livestock that highlighted its devastating impact on sheep flocks under new environmental conditions. These events, driven by the global trade in ruminants during colonial expansion, prompted detailed investigations, including Veglia's seminal 1915 study in South Africa that elucidated the worm's life cycle and economic toll.^[6]^[7]^[8]

Economic and veterinary significance

Haemonchus contortus imposes substantial economic burdens on the global livestock industry, with annual losses estimated in the billions of dollars (as of 2024) due to reduced productivity in small ruminants such as sheep and goats. These losses primarily stem from decreased weight gain, diminished milk production, and increased mortality rates, particularly in tropical and subtropical regions where small ruminant farming predominates in developing countries. For instance, heavy infections can reduce weight gain by 20-50%, exacerbating financial strain on farmers reliant on these animals for livelihood.^[9]^[10] Veterinarily, H. contortus is the primary cause of haemonchosis, a severe condition characterized by anemia and bottle-jaw edema resulting from the parasite's blood-feeding habits. This disease affects hundreds of millions of small ruminants worldwide, leading to clinical signs such as weakness, hypoproteinemia, and potentially fatal outcomes in untreated cases. Beyond direct health impacts, H. contortus serves as a key model organism for investigating anthelmintic resistance and host-parasite dynamics, facilitating advancements in parasite control strategies across nematode species.^[11]^[12]^[13]^[14] The parasite's prevalence contributes to food insecurity in tropical regions by undermining small ruminant production, which is vital for protein sources and income in resource-limited communities. It also influences sustainable farming practices through the promotion of integrated parasite management to mitigate resistance. Furthermore, H. contortus intersects with One Health approaches, as its transmission dynamics in livestock and wildlife reservoirs highlight the need for cross-sectoral strategies to address emerging resistance and ecological interactions.^[15]^[16]

Taxonomy and phylogeny

Classification

Haemonchus contortus is a parasitic nematode classified in the kingdom Animalia, phylum Nematoda, class Chromadorea, order Rhabditida, family Trichostrongylidae, genus Haemonchus, and species contortus.^[17] This placement situates it among the strongylid nematodes, a group characterized by their cylindrical bodies, complete digestive systems, and parasitic lifestyles in vertebrate hosts.^[18] As a member of the Trichostrongylidae family, H. contortus is distinguished from closely related congeners, such as H. placei, primarily through morphological features of the synlophe—a system of longitudinal cuticular ridges along the body—and male reproductive structures. In H. contortus, the synlophe typically features 30 ridges at the esophago-intestinal junction, compared to 34 ridges in H. placei, with variations in ridge arrangement and size contributing to species-specific patterns observable via scanning electron microscopy.^[19] Additionally, male spicules in H. contortus are shorter on average (mean length approximately 0.40–0.45 mm) than those in H. placei (mean length 0.50–0.55 mm), accompanied by differences in barb lengths and overall curvature, though some overlap in measurements necessitates complementary molecular or host-specific analyses for definitive identification.^[20] The nomenclature of H. contortus traces back to its original description as Strongylus contortus by Rudolphi in 1803, reflecting early classifications within the broader strongyle group. The genus Haemonchus was subsequently established by Cobb in 1898 to accommodate blood-feeding abomasal parasites, separating it from other strongylids based on anatomical traits like the distinctive barber's pole appearance in females due to ingested blood. The current binomial name was formalized and stabilized under the International Code of Zoological Nomenclature (ICZN) during the 20th century, resolving ambiguities from prior synonyms and ensuring consistent usage in veterinary parasitology.^[21]

Evolutionary history

Haemonchus contortus belongs to the superfamily Trichostrongyloidea within the order Strongylida, specifically in the subfamily Haemonchinae, where it forms a monophyletic group recognized as a sister taxon to the Ostertagiinae based on morphological and molecular analyses.^[22] Its closest relatives include H. placei, a parasite primarily of cattle, and H. similis, which infects both cattle and sheep; these species cluster together in phylogenetic trees derived from mitochondrial DNA (mtDNA) sequences such as cytochrome c oxidase subunit 1 (cox1) and nuclear ribosomal internal transcribed spacer 2 (ITS-2), as well as multi-locus datasets.^[22] Analyses of mtDNA and nuclear loci indicate that the divergence among these Haemonchus species occurred approximately 5–10 million years ago, coinciding with the Miocene-Pliocene radiation of ruminant hosts in Africa.^[22] The evolutionary diversification of H. contortus traces back to origins in African wild ruminants, particularly antelopes in sub-Saharan savannas, during the late Tertiary and Quaternary periods, where initial host-parasite associations developed amid ecological shifts and ungulate dispersals from Eurasia.^[23] From these ancestral lineages, H. contortus underwent multiple expansions facilitated by human-mediated livestock trade, transitioning from wild to domestic hosts like sheep and goats, with genetic evidence from cox1 and ITS-2 markers revealing ancient African-Asian lineages that predate widespread domestication.^[23] This diversification reflects host-switching events across artiodactyl families, including Bovinae and Caprinae, without strict cospeciation, as supported by phylogenetic reconstructions integrating morphological and molecular data.^[23] Biogeographically, the pre-colonial distribution of H. contortus was confined to Africa and parts of Asia, aligned with the ranges of its wild ruminant hosts and early domestic herds.^[23] Following European colonization after the 15th century, the parasite achieved global dissemination through the transcontinental movement of livestock, reaching the Americas, Australia, and Oceania via trade routes and agricultural expansions.^[23] Recent studies from 2019 to 2024, utilizing whole-genome sequencing and SNP analyses, further link contemporary genetic diversity patterns in H. contortus to historical human migration events, such as the trans-Atlantic slave trade and colonial sheep introductions, highlighting ongoing gene flow shaped by anthropogenic factors.^[24]

Biology

Morphology

Haemonchus contortus adults are cylindrical nematodes, with females measuring 18-30 mm in length and males 10-20 mm.^[1]^[25] The worms exhibit a characteristic "barber's pole" appearance due to the white, egg-filled uterus of the female spiraling around the red, blood-filled intestine.^[1]^[26] The mouth is equipped with a dorsal lancet in the buccal cavity, which pierces the host's abomasal mucosa to facilitate blood feeding.^[1] The cuticle features a synlophe consisting of 22-30 longitudinal ridges, varying by body region, with approximately 30 ridges at the esophago-intestinal junction and 22 at the mid-body.^[27]^[28] Sexual dimorphism is pronounced, with females possessing a vulva located near the tail end and males featuring a well-developed copulatory bursa supported by rays in a 2-2-1 pattern, including a Y-shaped dorsal ray.^[1]^[25] Male spicules are short and wedge-shaped, measuring 0.4-0.6 mm in length, with a barbed tip, and are guided by a gubernaculum during copulation.^[1]^[29]^[30] The eggs of H. contortus are oval and thin-shelled, measuring 70-90 μm in length by approximately 44 μm in width, typically containing 16-32 cells in early cleavage stages.^[1]^[31] The developmental stages include first-stage larvae (L1) that hatch from eggs, followed by second-stage larvae (L2), with both progressively increasing in size; there is no rhabditiform stage.^[1] The third-stage larvae (L3) are the infective form, ensheathed and larger than preceding stages, measuring around 500-600 μm in length.^[1]^[32]

Life cycle

Haemonchus contortus exhibits a direct life cycle without an intermediate host, involving both free-living and parasitic phases. Adult worms reside in the abomasum of the host, where females produce 5,000 to 10,000 eggs per day. These eggs are passed in the feces and embryonate under suitable environmental conditions.^[33]^[34] In the free-living phase, eggs hatch into first-stage larvae (L1) within 24 to 48 hours at temperatures between 20°C and 30°C. The L1 larvae then develop into second-stage larvae (L2) in approximately 24 to 48 hours, followed by molting to the ensheathed third-stage larvae (L3), the infective stage, which occurs over 5 to 7 days, completing larval development in 5 to 10 days total. The L3 larvae are resilient and can survive 3 to 6 months on pasture under moist conditions with optimal temperatures of 10°C to 30°C and relative humidity above 50%. Development arrests below 6°C or above 36°C, limiting the cycle in extreme climates.^[34]^[35]^[36] Infection begins when grazing hosts ingest L3 larvae from contaminated pasture. Upon reaching the rumen, the L3 larvae exsheath and migrate to the abomasum, where they develop into fourth-stage larvae (L4) within 2 to 3 days. The L4 then mature into adults over the next 15 to 18 days, with the prepatent period—the time from infection to egg-laying—ranging from 19 to 23 days. In temperate regions, L4 larvae may enter hypobiosis, a state of arrested development or diapause, to overwinter in the host's abomasum, resuming development when conditions improve in spring.^[37]^[33]^[38]

Genetics and genomics

Genome characteristics

The genome of Haemonchus contortus spans approximately 283 megabases (Mb) and is organized into five autosomes and one X chromosome. The initial draft assembly, reported in 2013, totaled 370 Mb including gaps, while improved chromosome-scale versions from a 2019 New Zealand isolate and a 2020 reference refined the assembled size to 283 Mb. A 2024 chromosome-contiguous assembly for the Haecon-5 strain further refined this to ~280 Mb. These assemblies predict 19,000–24,000 protein-coding genes, with the 2020 version annotating 19,489 nuclear genes encoding 20,987 transcripts and the 2024 version predicting 19,234 protein-coding genes.^[39]^[40]^[41]^[42] Key structural features include a GC content of 42.9%, yielding an AT-biased composition of 57.1%, and substantial repetitive elements comprising about 36% of the sequence. The genome shows extensive gene family duplications, notably in detoxification-related loci such as ABC transporters (approximately 50 genes) and cytochrome P450s, as well as immune evasion genes like expanded cathepsin B-like peptidases (63 copies). Telomeres consist of the repeat motif TTAGGC, facilitating chromosome end protection.^[39]^[40]^[43] Transcriptomic analyses using RNA-seq have generated extensive data on gene expression across life stages, identifying around 11,000 unique transcripts in early studies. These reveal stage-specific patterns, such as upregulation of over 120 peptidase genes and gut-enriched transcripts in blood-feeding adult stages, contrasting with embryonic expression of channels and transcription factors.^[44] Functional annotations emphasize genes critical for parasitism, including hemoglobinases (e.g., aspartic proteases like HcGALP) for digesting host blood and anticoagulants (e.g., HcSRCR1) to prevent clotting. A 2024 machine learning-based study predicted 1,754 essential genes (probability >0.5), prioritizing over 1,500 as CRISPR-Cas9 targets for validating core biological functions.^[39]^[45]

Population genetics

Haemonchus contortus exhibits high genetic diversity, characterized by substantial nucleotide variation in mitochondrial and ribosomal markers. Studies using mitochondrial cytochrome c oxidase subunit 1 (cox1) and internal transcribed spacer 2 (ITS-2) sequences have identified 77 cox1 haplotypes and 19 ITS-2 genotypes across populations, with nucleotide diversity (π) approximately 0.01 for mitochondrial DNA, including π = 0.029 for cox1 and π = 0.0103 for ITS-2.^[46] This diversity reflects panmixia within individual farms, where local populations show little genetic differentiation, but reveals structured variation at broader scales due to geographic isolation.^[47] Global population structure of H. contortus is shaped by historical human activities, particularly livestock trade, resulting in distinct clades associated with regions such as Africa, Europe, Asia, and the Americas. A comprehensive genomic survey of 223 individuals from 19 isolates across five continents identified three primary genetic clusters—Subtropical African, Atlantic, and Mediterranean/Oceanian—with subclades reflecting post-colonial dispersal via animal movement.^[48] Pairwise F_ST values between continental populations range from 0.06 to 0.42, indicating moderate to strong differentiation (mean F_ST ≈ 0.21–0.24), while global structure is quantified by N_ST = 0.59.^[48]^[47] Key drivers of this genetic variation include selective pressures from anthelmintic use, gene flow facilitated by host animal transport, and interspecific hybridization. Ivermectin resistance alleles are prevalent in over 50% of monitored populations worldwide, driven by selection on genes such as those in the glutamate-gated chloride channel family, contributing to reduced genetic diversity in resistant lineages.^[49]^[48] Gene flow via livestock trade maintains connectivity between regions, as evidenced by admixture patterns in Atlantic and Mediterranean clusters.^[48] Recent analyses have also documented hybridization with the closely related H. placei in mixed-host environments, potentially enhancing adaptive potential in sympatric populations.^[50] Effective population sizes for local isolates are estimated at approximately 10⁴, supporting rapid evolutionary responses to these forces.^[51]

Epidemiology

Global distribution

Haemonchus contortus is a cosmopolitan parasite primarily distributed in tropical and subtropical regions worldwide, with increasing presence in temperate zones up to approximately 55°N, endemic in Africa, Asia, Australia, and the Americas.^[24] It thrives in warm, humid environments suitable for ruminant hosts, showing high prevalence rates exceeding 50% in sheep and goats in areas such as sub-Saharan Africa and Brazil, where infection burdens can reach significant levels during favorable seasons.^[52] A 2025 meta-analysis reported a global pooled prevalence of 37% in ruminants.^[53] In contrast, its occurrence is sporadic in temperate zones, with outbreaks reported in regions like the United Kingdom and northern Europe due to milder winters, while it is largely absent from arid deserts and polar areas lacking sufficient moisture.^[38] The parasite's historical spread traces back to origins in sub-Saharan Africa around 2.5 to 10 thousand years ago, followed by dispersal through human activities including the transatlantic slave trade in the 1600s, which facilitated introduction to the Americas, and colonial livestock trade in the 1800s that brought it to Australia.^[24] Earlier expansions likely occurred via ancient trade routes such as the Silk Road into Asia, aligning with the domestication and movement of sheep and goats.^[48] Climatic factors strongly influence its distribution, with optimal conditions for larval development and survival at temperatures of 18–27°C and annual rainfall exceeding 800 mm, enabling free-living stages to persist on pasture.^[8] Species distribution modeling using MaxEnt has demonstrated strong correlations between H. contortus prevalence and host ruminant density, particularly in humid tropics.^[54] Climate change is expected to alter its distribution, with 2025 projections indicating potential suppression of transmission potential across most subregions of Africa but heightened risks of northward range expansion in temperate areas.^[55]

Host range and transmission

_Haemonchus contortus primarily infects small ruminants, with sheep (Ovis aries) and goats (Capra hircus) serving as the main hosts, where it exhibits high pathogenicity, particularly in young lambs and kids due to their developing immune systems and greater susceptibility to blood loss from the parasite's hematophagous feeding.^[56]^[57] Secondary hosts include cattle, which typically harbor low parasite burdens and show limited clinical impact, as well as wild ruminants such as white-tailed deer, bison, antelope, and giraffes, and camelids like llamas and other New World camelids, where infections can occur but are less common and often less severe.^[56]^[57]^[58] The parasite rarely infects horses or non-ruminant species, reflecting its strong host specificity to ruminants.^[56]^[59] Transmission occurs exclusively through the oral route, where grazing animals ingest infective third-stage larvae (L3) that have developed from eggs deposited in feces and migrated onto pasture vegetation or contaminated feed and water sources.^[60]^[57] There is no evidence of vertical transmission from dam to offspring.^[60] Infection rates peak during wet seasons in warm, humid environments, as moisture facilitates the survival and vertical migration of L3 larvae up grass blades, increasing their availability for ingestion.^[60]^[8] Several risk factors exacerbate transmission and outbreak severity. Overstocking leads to concentrated fecal contamination on pastures, elevating L3 exposure, while mixed grazing with multiple ruminant species can facilitate cross-transmission between hosts.^[60]^[8] Introducing naive animals, such as young or relocated stock without prior exposure, heightens infection risk due to their lack of immunity.^[8] Additionally, hypobiotic fourth-stage larvae (L4) can arrest development within the host during unfavorable conditions like winter, resuming activity in spring to cause sudden outbreaks.^[60]^[57]

Pathogenicity and disease

Mechanisms of pathogenesis

Haemonchus contortus adults and fourth-stage larvae (L4) are hematophagous nematodes that reside in the abomasum of ruminants, where they cause damage primarily through blood-feeding. The worms use a lancet-like cutting plate in their buccal cavity to pierce the abomasal mucosa, creating wounds that facilitate blood ingestion. Each adult worm consumes approximately 0.03–0.05 mL of blood per day, leading to substantial cumulative loss in infections with multiple parasites.^[61] To prevent clotting at the feeding site and within their digestive tract, H. contortus secretes a suite of anticoagulants, including serpins and prolyl-carboxypeptidases that inhibit the host's coagulation cascade.^[62]^[63] Additionally, the parasite produces hemoglobinases, such as cysteine proteases, to break down ingested hemoglobin into usable peptides and amino acids, supporting its own nutrition while exacerbating host blood depletion.^[64] The blood-feeding activity results in direct nutrient losses for the host, including iron and plasma proteins, which contribute to the development of anemia and hypoproteinemia. In heavy infections exceeding 5,000 worms, daily blood loss can reach 0.2–0.6 L, representing a significant fraction of the host's total blood volume and leading to iron deficiency anemia.^[60] Hypoproteinemia arises not only from blood loss but also from increased vascular permeability and protein exudation at the wound sites, further compounding nutritional deficits. Pathogenic effects intensify with worm burden; burdens above 100–200 worms typically induce mild anemia in young lambs, while over 1,000 worms can cause severe, life-threatening anemia in adults, particularly in smaller ruminants under 20 kg.^[60] Larval stages contribute to initial pathogenesis by migrating through the abomasal wall, inducing local inflammation and tissue disruption during establishment.^[64] H. contortus employs sophisticated immune evasion and modulation strategies to persist in the host. Excretory-secretory products (ESPs), released by the parasite, include immunomodulatory molecules that suppress host Th2 immune responses, such as those involving eosinophils and mast cells, thereby reducing effective expulsion.^[65] For instance, ESPs can inhibit dendritic cell maturation and T-cell activation, dampening pro-inflammatory cytokine production. During invasion, L3 larvae secrete hyaluronidase, an enzyme that degrades hyaluronic acid in the host's extracellular matrix, facilitating tissue penetration and establishment; suppression of this enzyme via RNA interference significantly reduces larval invasion rates in ovine abomasal explants and lowers adult worm burdens in vivo. These mechanisms collectively enable chronic infections, with larval migration further promoting inflammatory responses that, while initially host-protective, are often overwhelmed in susceptible animals.^[64]

Clinical signs and pathology

Haemonchosis, the disease caused by Haemonchus contortus infection, manifests primarily through severe anemia resulting from blood loss, leading to pale mucous membranes, lethargy, and weakness in affected sheep and goats.^[4] In acute cases, animals exhibit weight loss, exercise intolerance, and submandibular edema known as "bottle jaw" due to hypoproteinemia.^[60] Chronic infections often present with poor growth rates, reduced body condition, and occasional diarrhea, particularly in young lambs, where mortality can be high with fecal egg counts exceeding 5,000 eggs per gram.^[66] Gross pathological changes are most evident in the abomasum, where hyperemia, petechial hemorrhages, raised nodules containing adult worms, and pools of unclotted blood are common findings.^[67] Hepatic congestion and splenomegaly occur secondary to anemia and hemolysis, with the spleen showing increased macrophage activity.^[60] Histopathologically, the abomasal mucosa displays erosion, loss of parietal cells, submucosal edema, and marked infiltration of eosinophils in the lamina propria and submucosa, reflecting an inflammatory response.^[67] Host immune responses to H. contortus involve initial production of IgA and IgE antibodies in the abomasal mucosa, which can limit worm establishment but often wane over time, allowing persistent infections.^[4] Certain breeds, such as St. Croix hair sheep, demonstrate genetic resistance with lower worm burdens and fecal egg counts compared to susceptible breeds like Dorper.^[68] The FAMACHA© system, which scores conjunctival color on a 1-5 scale, identifies severe anemia in cases scoring 4-5, guiding targeted treatment.^[4] Disease progression varies by infection intensity and host status; peracute haemonchosis in naive lambs can lead to sudden death within 1-2 weeks from massive blood loss at rates of 0.03–0.05 mL per worm per day.^[60] Subacute forms involve intermittent clinical signs over 4-6 weeks, with phases of mild anemia, partial recovery through erythropoiesis, and eventual severe debilitation if untreated.^[4]

Diagnosis

Diagnostic methods

Diagnosis of Haemonchus contortus infections primarily relies on a combination of clinical assessments, parasitological techniques, and molecular methods to detect and quantify the parasite in ruminant hosts, particularly sheep and goats. Fecal egg counts (FECs) using the McMaster flotation technique serve as a cornerstone for quantitative assessment, where egg counts exceeding 200 eggs per gram (epg) typically indicate active infection.^[69]^[70] This method involves mixing fecal samples with a flotation solution to separate eggs, which are then counted under a microscope within a specialized chamber; however, it only detects infections in the patent stage, approximately three weeks post-infection when adult worms begin producing eggs.^[71]^[72] For species-specific identification amid mixed strongyle infections, peanut agglutinin (PNA) staining targets the unique surface glycoproteins on H. contortus eggs, enabling differentiation from other trichostrongylids through fluorescence microscopy.^[73]^[74] Molecular approaches, such as polymerase chain reaction (PCR) targeting the internal transcribed spacer 2 (ITS-2) region or cytochrome c oxidase subunit 1 (cox1) gene, provide high specificity for confirming H. contortus presence in fecal or larval samples.^[46]^[75] Loop-mediated isothermal amplification (LAMP) assays, optimized in 2021, offer field-applicable detection with sensitivity exceeding 95% for low-burden infections.^[76] Clinical tools complement parasitological methods by assessing anemia, a hallmark of haemonchosis correlating with worm burden. The FAMACHA eye color chart evaluates conjunctival mucous membrane pallor on a five-point scale, with scores of 4 or 5 indicating severe anemia warranting further investigation.^[77]^[78] Packed cell volume (PCV) measurement via microhematocrit centrifugation confirms anemia when values fall below 15%, often signaling high worm loads.^[79]^[80] For prepatent detection before eggs appear, enzyme-linked immunosorbent assay (ELISA) targeting coproantigens—excretory/secretory products in feces—identifies infections as early as 5–8 days post-infection with high specificity.^[81]^[82] Advanced diagnostic strategies include real-time PCR assays developed in 2025 for diagnosing Haemonchus sp. infections and estimating their relative abundance in mixed infections from fecal samples, as used in fecal egg count reduction tests (FECRT), enhancing accuracy in mixed infections.^[83] Post-mortem examination involves abomasal incision and adult worm recovery, providing definitive identification through morphological features like the barber's pole appearance in females.^[60] A 2025 serum metabolomics study identified candidate biomarkers, such as altered amino acid profiles, for detecting subclinical infections via non-targeted liquid chromatography-mass spectrometry, offering potential for early intervention without fecal sampling.^[84] Despite their utility, these methods have limitations: FECs can overestimate H. contortus contributions in mixed infections with other strongyles, while PCR and advanced assays like LAMP or ELISA remain costly and logistically challenging for routine farm use.^[85]^[76]

Control and management

Anthelmintic treatments

Anthelmintic treatments are the primary method for controlling Haemonchus contortus infections in small ruminants, targeting the parasite at various life stages to reduce worm burdens and alleviate clinical disease.^[86] These drugs are administered prophylactically or therapeutically, with efficacy depending on dosage, route, and local resistance patterns. Common administration routes include oral drenches for systemic absorption and topical pour-ons for external application, allowing flexibility based on farm management practices.^[87] The main drug classes used against H. contortus include benzimidazoles, such as albendazole, which initially achieved efficacies of approximately 95-99% by binding to β-tubulin and disrupting microtubule function.^[87] Macrocyclic lactones, exemplified by ivermectin, target glutamate-gated chloride channels to cause paralysis, with similar high initial efficacy.^[88] Levamisole, an imidazothiazole, acts on nicotinic acetylcholine receptors to induce spastic paralysis, while the newer amino-acetonitrile derivative monepantel offers broad-spectrum activity through a novel mechanism involving membrane hyperpolarization, demonstrating up to 100% efficacy in susceptible populations.^[89] To optimize treatment and delay resistance, targeted selective treatment (TST) approaches, such as the FAMACHA© system, identify anaemic animals based on conjunctival mucous membrane color for individualized dosing, reducing overall drug use.^[90] The refugia strategy complements this by leaving a portion of the parasite population untreated, preserving genetic diversity and slowing resistance selection.^[91] Anthelmintic resistance in H. contortus is widespread, affecting all major drug classes due to intensive selective pressure from repeated treatments. Anthelmintic resistance in H. contortus is widespread across Australia, affecting multiple drug classes including benzimidazoles.^[92] Resistance mechanisms include point mutations in β-tubulin, notably the F200Y substitution, which reduces benzimidazole binding affinity.^[93] Recent studies indicate multi-drug resistance in a significant proportion of global isolates, complicating control efforts.^[94] Monitoring treatment efficacy relies on the fecal egg count reduction test (FECRT), which assesses post-treatment egg reduction; values exceeding 90% confirm susceptibility, while lower results signal resistance.^[95] Combination therapies, such as ivermectin plus levamisole, can restore efficacy to 90-99% against resistant strains by targeting multiple mechanisms, though outcomes vary by region and resistance profile.^[96]

Prevention strategies

Grazing management plays a crucial role in reducing the risk of Haemonchus contortus infection by disrupting the parasite's life cycle, particularly the development of infective third-stage larvae (L3) on pasture. Rotational grazing systems, where animals are moved to new pastures before significant larval buildup occurs, have been shown to lower nematode burdens compared to set stocking.^[97] Zero-grazing, or confining animals to feedlots with harvested forage, eliminates direct exposure to contaminated pastures and can effectively prevent reinfection.^[98] Allowing pastures to rest for periods exceeding six months enables the die-off of L3 larvae, which typically survive only 3-6 months under dry conditions, further minimizing transmission risk.^[99] Avoiding low-lying wet areas during grazing is also recommended, as these environments favor larval survival and migration onto grass blades.^[100] Nutritional strategies and selective breeding enhance host resistance to H. contortus, reducing the severity of infections without relying on chemical interventions. Providing high-protein diets, such as those supplemented with rumen-protected proteins, improves immune responses and lowers fecal egg counts in infected sheep and goats by supporting albumin levels and resilience.^[101] Selective breeding programs targeting low FAMACHA scores— a clinical tool assessing anemia via conjunctival color—identify and propagate animals with genetic resistance to haemonchosis, leading to herds with reduced parasite burdens over generations.^[102] Copper oxide wire particles (COWP), administered as a nutritional supplement at doses of 0.5-2 g for lambs, selectively target H. contortus by releasing copper ions that inhibit worm development, achieving up to 90% reduction in fecal egg counts and approximately 54% reduction in adult worm burdens without broad-spectrum effects on other nematodes.^[103] Biosecurity measures are essential for preventing the introduction and spread of H. contortus on farms. Quarantining new stock for at least 24-48 hours, combined with fecal egg count monitoring and cleaning of transport vehicles, limits the influx of infected animals or contaminated equipment.^[104] Multi-species grazing, such as alternating sheep or goats with cattle, dilutes H. contortus L3 on pasture since the parasite is host-specific to small ruminants and cattle do not sustain its development.^[105] Integrated approaches to H. contortus prevention combine multiple tactics into farm-specific plans, often modeled on Hazard Analysis and Critical Control Points (HACCP) frameworks to identify and mitigate infection risks proactively.^[106] Incorporating climate forecasting helps time grazing rotations to avoid peak larval transmission periods influenced by temperature and rainfall, as warmer, wetter conditions accelerate H. contortus development.^[107] Recent reviews emphasize maintaining refugia—untreated subpopulations of animals or pastures—to preserve anthelmintic susceptibility in parasite populations, integrating this with grazing and breeding for sustainable long-term control.^[108]

Vaccine development

The only commercially available vaccine against Haemonchus contortus is Barbervax, which contains native gut membrane glycoproteins H11 and H-gal-GP extracted from adult worms.^[34] This vaccine stimulates antibody production that targets the parasite's intestinal microvillar surface, disrupting nutrient uptake and reducing worm viability.^[109] In field trials, Barbervax achieves 60-80% efficacy in reducing fecal egg output and worm burdens in sheep and goats, though protection wanes over time, necessitating annual boosters to maintain antibody levels.^[34] Several candidate antigens have shown promise in preclinical studies for eliciting protective immunity. Recombinant Hc-galectin, a galactose-binding protein involved in parasite-host interactions, induced partial protection in goats, reducing fecal egg counts by up to 48% and worm burdens by 46% following vaccination with 200 µg doses.^[110] Enzymes in trehalose synthesis, such as HcTPS (trehalose-6-phosphate synthase), have demonstrated strong immunogenicity; 2025 goat trials reported 61-70% reductions in adult worm burdens and 64% in egg output after immunization with recombinant HcTPS or related HcGOB.^[111] Excretory-secretory proteins (ESPs), including those stimulated by TH-9 immune responses and hyaluronidase, are also under investigation; TH-9 ESPs enhance IL-9 production for protective Th9 responses, while hyaluronidase inhibition via RNAi reduced larval invasion by over 50% in sheep abomasal tissues, positioning these as potential vaccine targets.^[112]^[113] Recent advances focus on improving antigen stability and delivery for broader efficacy. A 2025 glycoengineered recombinant vaccine, produced in Hi5 insect cells to incorporate nematode-specific glycan epitopes (including H11 variants and GA1), achieved 25% worm burden reduction and 81% fecal egg count reduction in sheep challenge trials, surpassing non-engineered versions.^[114] The VPEAR (Vaccine Platform for Extended Antigen Release) implant, a polyethylene device with polyanhydride rods for sustained antigen delivery over months to years, induced long-term antibody responses lasting up to 47 weeks in sheep, significantly lowering worm burdens (by ~73%) and anemia upon challenge without immune tolerance.^[115] Proteomics-driven identification of hidden antigens, combined with 2024 essential gene knockouts, has revealed conserved intestinal proteins as novel targets, enhancing vaccine design against polymorphic strains.^[116]^[45] Vaccine development faces challenges, including variable efficacy (40-90%) attributed to H. contortus genetic polymorphism and host factors, which can reduce protection across isolates.^[34] Adjuvants like Quil A have been shown to boost IgG responses and protection in field vaccinations, yet no formulation achieves sterilizing immunity, with vaccines primarily reducing fecundity and establishment rather than fully eliminating infections.^[117]

References

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Haemonchus contortus - Nemaplex
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Haemonchosis in Sheep and Goats, Control Strategies and ... - NIH
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Haemonchus - an overview | ScienceDirect Topics
The two species, Haemonchus contortus and Haemonchus placei, are major ruminant parasites of veterinary importance. Haemonchus contortus (Rudolphi, 1803) ...
[4]
Haemonchosis: A Challenging Parasitic Infection of Sheep and Goats
The genus Haemonchus spp. was first described in 1803 by Karl Rudolphi [1] and includes blood-sucking nematode helminths. Taxonomically, the genus belongs to ...
[5]
Scanning electron microscopy of different vulval structures in a ...
Haemonchus contortus was first described by Cobb in 1898 for the species Strongylus contortus described by Rudolphi 1803 (Veglia, 1915). It belongs to the ...
[6]
Haemonchosis: A Challenging Parasitic Infection of Sheep and Goats
The paper reviews the challenges regarding haemonchosis, a common parasitic infection of sheep and goats, caused by Haemonchus parasites.
[7]
[PDF] 4 Parasitic Gastritis in Sheep: Haemonchosis - CABI Digital Library
Probably the earliest comprehensive investigation and description of H. contortus was in South Africa at the instigation of Sir Arnold. Theiler and subsequently ...
[8]
Haemonchus contortus: the then and now, and where to from here?
Haemonchus contortus, or Barber's pole worm, is a nematode that causes high fecundity and rapid development, and is a 'nemesis' for small ruminant production.Missing: etymology | Show results with:etymology
[9]
Prevalence and risk factors of Haemonchus contortus in small ...
Mar 19, 2025 · The damage caused by H. contortus results in billions of dollars in economic losses to the ruminant industry (Bakshi et al., 2021, Demarco, 2024 ...
[10]
[PDF] economic losses due to haemonchosis in sheep and goats
Economic losses are related to productivity indexes, in particular to decrease in body weight that can range from 20 to 60% [19].
[11]
Common Gastrointestinal Parasites of Small Ruminants
Periparturient rise occurs commonly with Haemonchus infections, and mature sheep and goats may develop fatal infections in late pregnancy and early lactation.
[12]
Haemonchosis - an overview | ScienceDirect Topics
Haemonchosis is one of the most significant parasitic diseases of livestock worldwide, affecting hundreds of millions of ruminants such as sheep and goats.
[13]
Sensitivity of Haemonchus contortus to anthelmintics using different ...
Apr 12, 2022 · Helminthiasis and resistance to commercial anthelmintic compounds are major causes of economic losses for livestock producers, resulting in ...Missing: global | Show results with:global
[14]
Haemonchus contortus as a paradigm and model to study ...
Sep 2, 2013 · Haemonchus contortus, a gastro-intestinal parasite of sheep, has shown a remarkable propensity to develop resistance to all the drugs used in its control.
[15]
Advances in the Development of Anti-Haemonchus contortus Vaccines
The gastrointestinal nematode parasite Haemonchus contortus (H. contortus) is a resident of tropical and subtropical regions worldwide that imposes ...
[16]
Anthelmintic resistance in soil-transmitted helminths: One-Health ...
Dec 20, 2023 · This review provides an overview of the major human and animal STHs, treatment of the STHs, AR development and drug-related factors contributing towards AR, ...
[17]
ADW: Haemonchus contortus: CLASSIFICATION
Haemonchus contortus · Ecdysozoa: pictures (6937) · Ecdysozoa: specimens (116) · Ecdysozoa: sounds (13). PhylumNematodaroundworms. Nematoda: information (1)
[18]
https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=6289
[19]
[PDF] The Identification of Haemonchus Species and Diagnosis of ... - CORE
For example, transverse sections at the level of the esophagealeintestinal junc- tion reveal the presence of 30 ridges in H. contortus and 34 in H. placei. ( ...<|control11|><|separator|>
[20]
Differentiation of Haemonchus placei from Haemonchus contortus ...
Haemonchus placei presented higher mean spicule and barb lengths than H. contortus (P<0.05). However, some measurements overlapped. For this reason, a ...
[21]
Haemonchus contortus | CABI Compendium
Identity. Preferred Scientific Name: Haemonchus contortus (Rudolphi, 1803). International Common Names. English. barber pole worm. barber's pole worm. stomach ...
[22]
[PDF] Phylogeny for Species of Haemonchus (Nematoda
contortus (Rudolphi, 1803), H. placei (Place, 1893), and H. similis, Travassos, 1914, that are globally distributed in domes- ticated Bovinae and Caprinae ...
[23]
[PDF] Evolution and Biogeography of Haemonchus contortus - CORE
Species of the genus Haemonchus Cobb, 1898 occur in this complex ... Revision of the genus Haemonchus Cobb, 1898 (Nematoda; · Trichostrongylidae).
[24]
The global diversity of Haemonchus contortus is shaped by human ...
Oct 22, 2019 · It can be speculated that the shared ancestry between Guadeloupian and Moroccan worm isolates might result from the introduction of Spanish ...Missing: etymology | Show results with:etymology
[25]
Morphological and molecular characterization of Haemonchus ... - NIH
Sep 22, 2023 · The study established the role of morphological and molecular features in identifying and differentiating H. contortus parasite at the local level.Missing: etymology | Show results with:etymology
[26]
Haemonchus contortus - an overview | ScienceDirect Topics
The adults are 10 to 30 mm in length, so they are grossly visible (largest of the worms found in abomasum). When fresh, worms are red because of their ...<|control11|><|separator|>
[27]
Host-specificity and morphometrics of female Haemonchus ...
Haemonchus placei females had the longest mean body length and mean tail length, whereas the H. contortus body and tail measurements were intermediate, and H. ...
[28]
Cuticular ridge patterns applied for identifying adult female worms of ...
Only Haemonchus contortus was identified and most worms had constant numbers of ridges at EI, 4 mm, and MB, namely 30, 26, and 22 ridges, respectively ...
[29]
Morphology and histochemistry of the spicules and gubernaculum of ...
A study has been made of the morphological, histological and histochemical characteristics of spicules and gubernaculum in Haemonchus contortus (Rud., 1803).
[30]
Haemonchus contortus | INFORMATION - Animal Diversity Web
Haemonchus contortus inhabit the abomasum ("fourth stomach") of ruminent animals. H. contortus has been found in humans in Brazil and Australia.Missing: etymology | Show results with:etymology
[31]
Under the Microscope: Common Large Animal Parasites in Europe
Feb 7, 2025 · The Haemonchus egg is oval and transparent, measuring about 70-85 microns in length. The larvae in the early stages (L1, L2) are also ...
[32]
Haemonchus contortus larvae illustrating the morphological features...
Haemonchus contortus larvae illustrating the morphological features distinguishing third stage larvae (L3), exsheathed L3 (xL3), and fourth stage larvae (L4). ...
[33]
Haemonchosis in Sheep and Goats, Control Strategies and ... - MDPI
Haemonchus contortus is a blood-sucking nematode that feeds on blood from capillaries in the abomasum of ruminants [4]. As a single worm ingests up to 50 µL of ...
[34]
Advances in the Development of Anti-Haemonchus contortus Vaccines
It was estimated that the annual economic losses caused by H. contortus to the livestock industry accounted for $30–300 million globally [2,3]. The primary ...
[35]
The development and mortality of the free-living stages of ...
No larval development occurred at 5°C; peak third-stage larval recovery occurred at 20°C.
[36]
A Review: Haemonchus contortus Infection in Pasture-Based Sheep ...
Optimal conditions for H. contortus egg hatching and larval development occur at ambient vegetation microclimate temperatures of 22 to 26 °C and humidity close ...
[37]
Meta-analysis of the parasitic phase traits of Haemonchus contortus ...
Apr 24, 2017 · This paper aimed to quantify the main life history traits of H. contortus and to identify explanatory factors affecting these traits using a powerful method.
[38]
Haemonchus contortus: an overview - 2023 - Wiley Online Library
Jan 6, 2023 · Haemonchus contortus is a blood-feeding parasite in sheep and goats, known as 'barber's pole worm', causing anemia, illthrift, and bottle jaw.Missing: etymology | Show results with:etymology
[39]
The genome and transcriptome of Haemonchus contortus, a key ...
Aug 28, 2013 · Here we report the detailed analysis of a draft genome assembly and extensive transcriptomic dataset for H. contortus.
[40]
Genomic and transcriptomic variation defines the chromosome ...
Nov 9, 2020 · Haemonchus contortus as a paradigm and model to study anthelmintic drug resistance. Parasitology 140, 1506–1522 (2013). Article CAS Google ...
[41]
Genome Sequence of the Anthelmintic-Susceptible New Zealand ...
Jul 2, 2019 · The genome sequence presented here is consistent with the genome architecture of the existing Haemonchus species and is a valuable resource for ...Materials And Methods · Genome Sequencing, Assembly... · Results And Discussion
[42]
The genome and developmental transcriptome of the strongylid ...
Aug 28, 2013 · We provide a draft of the genome and the transcriptomes of all key developmental stages of H. contortus to support biological and biotechnological research ...
[43]
Inference of Essential Genes of the Parasite Haemonchus contortus ...
Jun 27, 2024 · Most (85%) of the 499 high-priority essential genes predicted (probability of >0.7) were linked to autosomal chromosomes chr1–chr4 without a ...
[44]
Haemonchus contortus, an obligatory haematophagus worm ... - NIH
May 26, 2024 · Haemonchus contortus, a stomach worm, is prevalent in ruminants worldwide. They particularly hamper profitable small ruminant production.
[45]
Global patterns reveal strong population structure in Haemonchus ...
In contrast with previous findings on the population genetic structure of H. contortus, the calculation of population structure gave high values (Nst=0.59).Missing: effective size 10^
[46]
The global diversity of Haemonchus contortus is shaped by human ...
We have performed a survey of its genome-wide diversity using single-worm whole genome sequencing of 223 individuals sampled from 19 isolates spanning five ...Missing: Fst | Show results with:Fst
[47]
Drug Resistance in Human Helminths: Current Situation and ...
High prevalences of AR, often exceeding 50%, have now been reported in all parts of the world for gastrointestinal helminths of sheep, goats, and horses kept in ...<|separator|>
[48]
Extensive Countrywide Molecular Identification and High Genetic ...
Mar 8, 2024 · Haemonchus spp. is a blood-sucking nematode parasite, which was first described in 1803 by Karl Rudolphi [1]. It belongs to the superfamily ...<|separator|>
[49]
[PDF] The global diversity of the major parasitic nematode Haemonchus ...
Apr 13, 2019 · resistance loci in H. contortus, as supported by the functions of their C. elegans. 407 orthologs (snf-9, unc-24, B0361.4, aex-3 respectively) ...<|control11|><|separator|>
[50]
Ovine haemonchosis: a review | Tropical Animal Health and ...
Nov 20, 2020 · Prevalence. As per the latest information available, H. contortus originated from sub-Sahara, Africa, in wild ungulates and then evolved to ...
[51]
Potential distribution modelling for Haemonchus contortus (Nematoda
May 30, 2024 · The aim of this study was to model the potential distribution of H. contortus in South America. We used MaxEnt to perform the analyses.
[52]
[PDF] The Identification of Haemonchus Species and Diagnosis of ... - CORE
Haemonchus species can be identified using morphological approaches, molecular methods, and immunological methods for diagnosing haemonchosis.
[53]
[PDF] ROLE OF P-GLYCOPROTEIN IN HAEMONCHUS CONTORTUS ...
Haemonchus contortus was first described in 1803 by Rudolphi. The classification is as follows: Class Nematoda, Order Strongylida, and Family.
[54]
Pathology of Haemonchus contortus in New World camelids in the ...
Mar 10, 2016 · Haemonchus contortus is a gastrointestinal nematode that attaches to the abomasum of small ruminants (sheep and goats) and feeds on whole blood.<|separator|>
[55]
[PDF] Nematode Parasites of Small Ruminants, Camelids and Cattle
Haemonchus contortus, although a primary pathogen in sheep, can be transmitted to cattle but the more usual Haemonchus sp in cattle is Haemonchus placei.
[56]
A Review: Haemonchus contortus Infection in Pasture-Based Sheep ...
May 11, 2022 · Haemonchosis is an important cause of anaemia in sheep worldwide, particularly those that are kept in pasture-based systems in warm, high rainfall environments.
[57]
https://oaktrust.library.tamu.edu/bitstreams/dda2fde1-1aa5-4167-954d-c49c9b81b737/download
[58]
Complement and Coagulation Cascade Activation Regulates ... - MDPI
contortus produces a serpin with anticoagulant properties, which may contribute to its feeding mechanism. In vitro studies have shown that this serpin can ...Missing: HcAP | Show results with:HcAP
[59]
The intestinal contortin structure in Haemonchus contortus - PubMed
These data suggest that the prolyl-carboxypeptidases are intestinal anticoagulants used by H. contortus to interfere with blood coagulation.Missing: HcAP | Show results with:HcAP
[60]
https://pmc.ncbi.nlm.nih.gov/articles/PMC9137471/
[61]
Excretory/secretory proteins inhibit host immune responses by ...
Sep 26, 2022 · Previous studies showed that H. contortus escape the immunity through complex mechanisms, including releasing excretory/secretory proteins (ESPs) ...
[62]
[PDF] Review on Control of Haemonchus Contortus in Sheep and Goat
Jun 27, 2018 · Clinical signs: Haemonchus contortus infection is clinically diagnosed by anemia, dehydration, and sub mandibular internal fluid accumulation ...
[63]
Comparative study on pathological changes in sheep and goats ...
Jul 20, 2022 · Comparative study on pathological changes in sheep and goats experimentally infected with Haemonchus Contortus | Surgical and Experimental ...
[64]
Resistance of St. Croix lambs to Haemonchus contortus in ... - PubMed
In experimental infections, St. Croix lambs developed significantly greater levels of resistance to H. contortus, following primary exposure, as compared with ...Missing: FAMACHA score
[65]
Fecal egg counting - ACSRPC
A fecal egg count (FEC) is a quantitative measure of how many worm eggs a sheep/goat is passing in each gram of its manure. You get a number like 1000 EPG ...
[66]
[PDF] Fecal Egg Counting for Sheep and Goat Producers
A fecal egg count (FEC) is a simple procedure you can perform at home to get an approximation of the parasite load your sheep or goats are carrying.<|separator|>
[67]
[PDF] How To Do The Modified McMaster Fecal Egg Counting Procedure
This is a flotation test that separates parasite eggs from debris based on density; the eggs float to the surface of the counting chamber. This test uses a ...Missing: Haemonchus | Show results with:Haemonchus
[68]
Guide to Performing McMaster's Fecal Egg Count for Small Ruminants
Dec 23, 2024 · Weigh and mix: Weigh 4 grams of feces and mix with 56 mL of flotation solution. Strain: Strain the mixture to remove large debris. Fill the ...Missing: contortus | Show results with:contortus
[69]
Egg autofluorescence and options for detecting peanut agglutinin ...
Feb 16, 2019 · Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples.
[70]
Lectin staining of trichostrongylid nematode eggs of sheep - PubMed
Lectin staining of trichostrongylid nematode eggs of sheep: rapid identification of Haemonchus contortus eggs with peanut agglutinin. Int J Parasitol. 1996 ...
[71]
Genetic diversity and population structure of Haemonchus contortus ...
This study was conducted to elucidate the genetic diversity and population structure of this nematode in Thailand based on mitochondrial DNA markers.
[72]
Point of care colourimetric and lateral flow LAMP assay for ... - Parasite
Dec 15, 2021 · In this study, we present an optimised colourimetric and a lateral flow LAMP assay for the detection of Haemonchus contortus in small ruminant faecal samples.
[73]
Validation of the FAMACHA eye color chart for detecting clinical ...
Aug 13, 2004 · The FAMACHA method is an extremely useful tool for identifying anemic sheep and goats in the southern US and US Virgin Islands.
[74]
Validation of the FAMACHA© eye color chart for detecting clinical ...
The FAMACHA method is an extremely useful tool for identifying anemic sheep and goats in the southern US and US Virgin Islands.
[75]
The Varying Relationship Between Packed Cell Volume and Fecal ...
Packed cell volume (PCV) and fecal egg count (FEC) have been used as indirect and direct indicators of haemonchosis, respectively.
[76]
[PDF] Comparative Assessment of Coproscopy and Packed Cell Volume ...
Aug 21, 2024 · For animals with positive FEC results, larval cultures and Packed cell volume tests were performed to confirm the presence of Haemonchus ...
[77]
Detection of Haemonchus contortus surface antigen in faeces from ...
An antigen capture enzyme-linked immosorbent assay (ELISA) has been developed that could identify Haemonchus contortus antigens in dilute suspensions of worm ...
[78]
Dynamics of Haemonchus Contortus Coproantigen Appearance in ...
At 5-8 days post infection (DPI), antigen capture- ELISA detected H. contortus coproantigen in feces of the experimentally infected lambs. Diagnostic detection ...Missing: coproantigens | Show results with:coproantigens
[79]
Use of a Novel Real-Time PCR to Investigate Anthelmintic Efficacy ...
Jun 10, 2025 · This study presents the development of a novel real-time PCR method for diagnosing Haemonchus sp. infections in grazing ruminants.
[80]
Candidate serum metabolite biomarkers of subclinical Haemonchus ...
Oct 1, 2025 · Therefore, we conducted a controlled longitudinal study to identify predictive biomarkers of Haemonchus contortus infection in 60 Rideau Arcott ...
[81]
Comparison of Four Diagnostic Methods for Detection and Relative ...
Jan 24, 2018 · In conclusion, H. contortus can be identified by McMaster counting, without major mistakes regarding false positive results. However, molecular ...
[82]
Sertraline as a new potential anthelmintic against Haemonchus ...
Dec 11, 2021 · Four major classes of common anthelmintics are routinely administered to livestock: benzimidazoles (e.g. mebendazole, albendazole, fenbendazole, ...
[83]
Anthelmintic Resistance and Its Mechanism: A Review - PMC - NIH
Dec 15, 2021 · The efficiency of modern anthelmintics is about 99% against vulnerable strains. A small number of tolerant parasites are the most resistant ...
[84]
Anthelmintic Resistance in Haemonchus contortus
Resistance has been further defined in terms of drug concentrations which inhibit a certain aspect of development or motility in 50% or 99% of the population in ...
[85]
Efficacy of monepantel and anthelmintic combinations against ...
Study 3 was also a FECR test that resulted in efficacies of 100% for monepantel and 83.0% for a formulated 4-way combination of abamectin + levamisole + ...
[86]
Evaluation of the FAMACHA© system for targeted selective ...
The FAMACHA© system is a practical on-farm method, which can be used to identify small ruminants in need of anthelmintic treatment, depending on the colour ...
[87]
Refugia and anthelmintic resistance: Concepts and challenges - PMC
May 17, 2019 · While the use of refugia emerges as a potential control strategy against both Bacillus thuringiensis (Bt) insect resistance and anthelmintic ...
[88]
The current status of anthelmintic resistance in a temperate region of ...
The prevalence of anthelmintic resistance to any product was high (71%), with farms reporting varying levels of drug efficacies (21–100%). Resistance to older ...
[89]
Assessment of the F200Y mutation frequency in the β tubulin gene ...
Jul 18, 2020 · All things considered, we show that the F200Y mutation is still a viable and reliable marker for the detection and surveillance of benzimidazole ...
[90]
Multidrug resistance in Haemonchus contortus in sheep - can it be ...
Dec 19, 2023 · Resistance to ALB and IVM in Haemonchus contortus was confirmed. Alternative approaches to improve the efficacies of benzimidazole did not sufficiently ...
[91]
How to Conduct a Fecal Egg Count Reduction Test (FECRT)
If the efficacy of the anthelmintic falls above 90% – good news, your program is working. If your FECRT results fall below 90%, here's what to do next:.<|control11|><|separator|>
[92]
Overcoming macrocyclic lactone resistance in Haemonchus ...
Sheep were slaughtered at Day 135 for estimates of total worm burden. FWEC of sheep treated with IVM + LEV declined (99.9% reduction) after administration of ...
[93]
[PDF] Managing Haemonchosis in Sheep and Goats
Haemonchus infections can cause severe anemia and protein loss, which can lead to economic losses such as lowered milk production, poor weight gains, ...Missing: symptoms edema
[94]
Intensive rotational grazing assists control of gastrointestinal ...
In common with other high-risk situations, effective H. contortus preventative strategies include pasture management to avoid excessive infective larvae ...
[95]
Haemonchus - Sheep Diseases - Farm Health Online
Bottle jaw is a classic sign of haemonchosis as fluid builds up underneath the lower jaw. Infection with Haemonchus can be categorised as either acute, sub ...Missing: edema | Show results with:edema
[96]
[PDF] The Effect of Management Strategies on Haemonchus Contortus ...
This may have triggered the larvae to enter hypobiosis, the dormant stage of their life cycle inside the abomasum of the sheep and goats. While they are in ...
[97]
Influence of dietary protein supply on resistance to experimental ...
The most significant finding was that a high-protein diet was associated with increased resistance to infection by H. contortus, based on FEC and worm burden, ...Missing: prevention | Show results with:prevention<|control11|><|separator|>
[98]
(PDF) The FAMACHA© system for managing haemonchosis in ...
Aug 7, 2025 · The main benefits of the system are the reduction in treatments, its use for discriminating between animals of varying ability to cope with infection.
[99]
Effectiveness of copper oxide wire particles for Haemonchus ...
Mar 10, 2008 · In experiment 2, faecal worm egg counts at 4 and 6 weeks were reduced by more than 90% in the COWP treated lambs and worm numbers were 54% lower ...Missing: breeding | Show results with:breeding
[100]
Overview of Gastrointestinal Parasites of Ruminants
The administration of copper oxide wire particles as a bolus has been shown to decrease the Haemonchus contortus worm burden in sheep and goats; however, care ...
[101]
Multi-species Grazing for Control of Gastrointestinal Nematodes
Multi-species grazing, alternate grazing of cattle with sheep or goats, offers benefits to gastrointestinal nematode (GIN) control as GIN species differ ...Missing: biosecurity quarantine
[102]
Controlling nematode infections in sheep: application of HACCP
Oct 5, 2018 · This article examines the challenge of nematode infections and anthelmintic resistance and suggests how hazard analysis and critical control point (HACCP) ...
[103]
A Review of the Impact of Climate Change on the Epidemiology of ...
In ruminants, free living stages of gastrointestinal (GI) nematodes, such as Haemonchus contortus, are sensitive to temperature and humidity changes; thus, it ...
[104]
Management of parasite refugia for Haemonchus contortus through ...
Apr 30, 2018 · One strategy is the introduction of susceptible parasites in refugia when these refugia are at low levels. This strategy could lead to a renewed ...
[105]
Barbervax
Barbervax Controls Barber's Pole worm. Barbervax Environmentally friendly. No chemicals. Suppresses worm egg production, cleans up your pasture.How to use · Vaccination schedule · Buy · About
[106]
Vaccination of goats with recombinant galectin antigen induces ...
Vaccination of goats with recombinant galectin antigen induces partial protection against Haemonchus contortus infection. Parasite Immunol. 2007 Jun;29(6): ...Missing: Hc- | Show results with:Hc-
[107]
Enzymes involved in trehalose-chitin synthesis in Haemonchus ...
Feb 20, 2025 · These findings indicate that rHcTPS and rHcGOB exhibit superior immune protective effects, rendering them promising candidates for vaccine development.
[108]
TH-9 stimulated ESPs as a potential candidate for Vaccine ...
A Comprehensive Review on Haemonchus contortus Excretory and Secretory Proteins (HcESPs): TH-9 stimulated ESPs as a potential candidate for Vaccine Development ...Missing: hyaluronidase | Show results with:hyaluronidase
[109]
Suppression of hyaluronidase reduces invasion and establishment ...
Aug 27, 2020 · Hyaluronidase plays a key role in host's tissue invasion and larval establishment, and it is used as a virulence factor by H. contortus while establishing the ...Missing: modulation | Show results with:modulation
[110]
Safety and efficacy of a novel glycoengineered recombinant vaccine ...
Aug 11, 2025 · Haemonchus contortus is a highly pathogenic nematode in small ruminants, causing major production losses and animal welfare concerns.Missing: global | Show results with:global
[111]
Implantation of a vaccine platform for extended antigen release ...
Apr 9, 2025 · Implantation of a vaccine platform for extended antigen release (VPEAR) induces long-term immunity against Haemonchus contortus in sheep | ...Vaccine Formulations · H. Contortus Elisa · Results
[112]
Investigation of the combined efficacy of two Haemonchus contortus ...
The combination of a vaccine using hidden antigen, with one based on H. contortus larval surface antigens (HcsL3) may improve vaccinal protection in weaner ...Research Paper · Introduction · Worm Egg Count
[113]
The Effect of Different Adjuvants on Immune Parameters and ...
Oct 21, 2013 · In the present study, several adjuvants were evaluated for their ability to protect sheep after field vaccination with the larval-specific Haemonchus contortus ...Missing: polymorphism sterilizing