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Methanobrevibacter smithii

Methanobrevibacter smithii is a hydrogenotrophic methanogenic archaeon belonging to the family Methanobacteriaceae within the domain Archaea, phylum Methanobacteriota, class Methanobacteria, and order Methanobacteriales.^[1] It is the most abundant archaeon in the human gastrointestinal tract, particularly in the distal colon, where it comprises up to 10–14% of the anaerobic microbial population and plays a pivotal role in the gut ecosystem by consuming hydrogen produced during bacterial fermentation of dietary polysaccharides and reducing it with carbon dioxide to form methane.^[2]^[3] Morphologically, M. smithii consists of ovoid or coccoid cells measuring approximately 0.5 μm in diameter, often occurring in pairs, short chains, or small clusters.^[3] Its genome, fully sequenced in 2007, is 1,853,160 base pairs long and contains 1,795 protein-coding genes, including those encoding surface glycans that mimic host mucosa for adherence, adhesin-like proteins for bacterial symbiosis, and enzymes for utilizing formate and other substrates beyond hydrogen.^[2] This genetic makeup enables M. smithii to thrive in the anaerobic, nutrient-limited environment of the human gut, where it forms syntrophic relationships with fermentative bacteria such as Bacteroides thetaiotaomicron and Ruminococcus species, enhancing the efficiency of carbohydrate breakdown and short-chain fatty acid production, which can contribute up to 10% of the host's daily caloric intake.^[2]^[4] M. smithii colonizes the human gut early in life, detected in 100% of gastric juice samples from one-day-old newborns and likely transmitted from maternal microbiota, establishing a lifelong presence with over 90% prevalence in adults.^[5]^[3] Its methanogenic activity produces approximately 0.35 liters of methane per day, which is expelled via breath, flatus, or feces, but dysregulated abundance has been implicated in various conditions, including constipation-predominant irritable bowel syndrome, obesity through increased energy harvest, colorectal cancer, and even extra-intestinal infections such as abscesses and archaemia in immunocompromised individuals.^[3]^[6]^[4] Recent studies have identified cell variants, including small and large forms potentially associated with symbiotic nanobacteria, highlighting its genomic diversity across 15 genotypes and ongoing adaptations in human physiology and pathology.^[4]

Taxonomy and discovery

Classification

Methanobrevibacter smithii belongs to the domain Archaea, phylum Methanobacteriota, class Methanobacteria, order Methanobacteriales, family Methanobacteriaceae, genus Methanobrevibacter, and species smithii.^[7] The phylum Methanobacteriota represents a recent reclassification from the former phylum Euryarchaeota, validated in 2023 to better reflect phylogenetic diversity among methanogenic archaea.^[8] This hydrogenotrophic methanogen, which reduces CO₂ with H₂ to produce methane, is defined by these traits within the order Methanobacteriales.^[9] The species name smithii derives from the New Latin genitive masculine noun honoring P.H. Smith, the microbiologist who isolated the organism; the type strain is DSM 861 (also designated ATCC 35061, OCM 144, and PS).^[9]^[7] The species was formally described as Methanobrevibacter smithii Balch and Wolfe 1981.^[7] Phylogenetically, M. smithii clusters closely with other Methanobrevibacter species, such as M. ruminantium, sharing approximately 92-95% 16S rRNA gene sequence similarity, reflecting their common ancestry among rumen and gut-associated methanogens.^[10] Human gut isolates exhibit >97% 16S rRNA similarity to the type strain, underscoring M. smithii's prevalence in the archaeal component of the intestinal microbiome.^[11] Post-2020 taxonomic updates have confirmed the stability of this placement within Methanobacteriota through phylogenomic analyses, including a 2023 proposal of a homotypic synonym ("Methanocatella smithii") that remains invalidly published.^[7]^[12]

Historical background

Methanobrevibacter smithii was first isolated from human feces in 1982 by Terry L. Miller and Meyer J. Wolin, who identified it as a hydrogen-utilizing methanogen capable of producing methane under anaerobic conditions.^[13] This isolation marked the initial recognition of a methanogenic archaeon in the human gut, highlighting its role in scavenging hydrogen produced by bacterial fermentation. The strain was obtained from enrichment cultures of fecal specimens from healthy adults, where methane production was observed within five days, confirming its methanogenic activity.^[13] The species was formally named Methanobrevibacter smithii in 1981 by William E. Balch and Ralph S. Wolfe, honoring Paul H. Smith for his contributions to methanogen isolation, including the type strain from anaerobic sewage digester sludge.^[9] Early studies in the 1980s focused on cultivating the organism from anaerobic gut environments, with Miller and Wolin developing selective media containing antibiotics like cephalothin and clindamycin to enumerate and isolate it from human feces, revealing populations up to 10^8 cells per gram.^[13] These efforts established M. smithii as the predominant methanogen in the human intestine, present in a significant portion of methane-producing individuals.^[14] In the 1990s, molecular techniques such as PCR began to facilitate detection of M. smithii in human samples, confirming its high prevalence and stability within the gut microbiota.^[15] For instance, PCR targeting 16S rRNA genes demonstrated that M. smithii accounted for the majority of archaeal sequences in fecal samples from methane-positive subjects, underscoring its ubiquity.^[16] A major milestone came in 2007 with the complete genome sequencing of the type strain ATCC 35061, the first for a human gut-derived methanogen, revealing a 1.85 Mb genome with approximately 1,795 protein-coding genes adapted for hydrogenotrophic methanogenesis.^[17] Post-2020 advancements in culturing techniques have expanded understanding of M. smithii's morphological diversity, including the isolation of filament-forming variants from human feces using targeted anaerobic media.^[18] A 2025 study isolated a novel strain, GRAZ-2, demonstrating variable rod shapes and filament formation, which enhances the cultivable human archaeome and provides new models for studying gut archaea.^[18]

Morphology and physiology

Cell structure

Methanobrevibacter smithii exhibits an irregular coccobacillus or short rod morphology, with cells typically measuring 0.5-1.5 μm in length and occurring as singles, pairs, or short chains; under certain conditions, filaments may form. These non-motile cells stain Gram-positive in fresh cultures, reflecting their archaeal cell wall composition.^[19]^[20]^[4] The cell wall of M. smithii consists of pseudopeptidoglycan, a rigid structure distinct from bacterial peptidoglycan, composed of N-acetylglucosamine and N-acetyltalosaminuronic acid linked by β-1,3-glycosidic bonds; this confers resistance to lysozyme and many peptidoglycan-targeting antibiotics. An S-layer contributes to the surface architecture, enhancing structural integrity in harsh environments.^[4]^[21]^[22] The plasma membrane forms a monolayer of ether-linked lipids, including glycerol diphytanyl tetraethers with branched isoprenoid chains, which provide enhanced stability in anaerobic conditions compared to bacterial ester-linked bilayers. This lipid composition renders the membrane sensitive to statins, as they inhibit HMG-CoA reductase, a key enzyme in archaeal lipid biosynthesis.^[4]^[23]^[24] M. smithii produces DNA-carrying extracellular vesicles (EVs) with diameters of 20-180 nm, enriched in histones, and DNA repair proteins; these EVs facilitate intercellular communication by exporting genetic material and modulating methanogenesis in the gut microbiome.^[25] Cell variants exist in M. smithii, including small (1.5-2 μm) and large forms associated with symbiotic Nanoarchaeota, differing in surface glycosylation and polysaccharides that aid gut adaptation; smooth and rough morphotypes further vary in surface features, influencing environmental interactions.^[4]

Metabolic processes

Methanobrevibacter smithii is a strict anaerobe that derives energy primarily through hydrogenotrophic methanogenesis, reducing carbon dioxide with hydrogen to produce methane via the reaction $4 \mathrm{H_2} + \mathrm{CO_2} \rightarrow \mathrm{CH_4} + 2 \mathrm{H_2O} (\Delta G^{\circ\prime} = -131 kJ/mol). This process involves a series of enzymatic steps, beginning with the activation of CO₂ by tungsten formylmethanofuran dehydrogenase (Fwd) to form formylmethanofuran, followed by subsequent reductions through intermediates like formyl- and methenyl-tetrahydromethanopterin, culminating in the terminal step catalyzed by the methyl-coenzyme M reductase complex encoded by the mcr operon (McrABG). The organism also employs F₄₂₀-reducing hydrogenases to activate H₂, facilitating electron transfer in the pathway.^[23]^[2] In addition to H₂/CO₂, M. smithii can utilize alternative substrates for methanogenesis, including formate via formate dehydrogenase (FdhCAB) and methanol through methanol:cobalamin methyltransferase (MtaB), though the latter is not actively used in standard cultures despite the presence of supporting genes. Carbon fixation occurs autotrophically via the Wood-Ljungdahl pathway, an acetyl-CoA cleavage pathway that assimilates CO₂ and produces acetate as a key byproduct, involving enzymes such as acetyl-CoA synthase (Acs), pyruvate:ferredoxin oxidoreductase (Por), and pyruvate carboxylase (Pyc). This pathway supports growth on minimal media supplemented with acetate.^[23]^[2]^[26] Energy conservation in M. smithii relies on chemiosmotic coupling during methanogenesis, where electrons from H₂ or formate are transferred via a membrane-bound electron transport chain involving methanophenazine as the quinone analog, without the involvement of cytochromes. This generates a proton motive force for ATP synthesis through an A₁A₀ ATP synthase, yielding approximately one-third of an ATP per methane molecule produced. Recent studies have highlighted how M. smithii influences co-culture dynamics by consuming excess H₂, thereby alleviating thermodynamic constraints on fermentative bacteria and enhancing short-chain fatty acid (SCFA) production through improved cross-feeding efficiency.^[23]^[2]

Ecology and distribution

Environmental habitats

Methanobrevibacter smithii is ubiquitous in various anaerobic environments, where it plays a key role as a hydrogenotrophic methanogen. It is commonly found in the rumen of ruminants such as cows and sheep, where it constitutes a significant portion of the archaeal community, often comprising up to 10% of the archaea and serving as one of the predominant methanogens.^[27]^[28] Additionally, M. smithii occurs in sewage sludge and anaerobic digesters, where it participates in organic matter decomposition under oxygen-depleted conditions.^[29] As a dominant methanogen in many oxygen-limited habitats, M. smithii exhibits high prevalence based on metagenomic analyses. It has been identified with variable prevalence in fecal samples from various animals, for example, 50% in pigs, 25% in dogs, 16.7% in cats, and 4.2% in sheep and horses, highlighting its distribution in host-associated anaerobic niches.^[30] Metagenomic surveys frequently detect M. smithii sequences in these environments, underscoring its ecological importance in facilitating interspecies hydrogen transfer and methanogenesis. M. smithii is a mesophilic archaeon with optimal growth at 37–38°C and pH 6.5–7.0, conditions typical of animal digestive tracts and other moderate anaerobic settings.^[31]^[32] It demonstrates tolerance to salinity up to 0.17 M NaCl (1% w/v), enabling survival in mildly saline anaerobic environments such as certain sewage treatments.^[32] In non-human contexts, M. smithii contributes significantly to global methane emissions through its role in ruminant digestion, where rumen fermentation by this methanogen accounts for a substantial fraction of enteric methane output from livestock.^[28] Recent metagenomic surveys from 2024 reveal M. smithii as a core species in anaerobic digester communities, with genetic variants showing adaptations to varying pH levels in extreme microsites.^[29]

Role in the human gut microbiome

Methanobrevibacter smithii is the most prevalent archaeon in the human gut microbiome, accounting for over 90% of archaeal sequences in fecal samples and detected in 95-100% of healthy adults. It constitutes approximately 0.1-10% of the total microbial community, with average relative abundances around 0.2-0.6% but reaching up to 6.8% in some individuals. Abundance is notably higher in adults compared to infants, where methanogenic archaea like M. smithii are less common early in life and gradually increase with age.^[33]^[34]^[21]^[35]^[36]^[37] Colonization by M. smithii typically begins in early childhood, often becoming established by school age, and persists throughout life in most individuals. It is primarily located in the colon and rectum, where it integrates into the stable adult gut microbiota. Detection in these regions relies on 16S rRNA gene sequencing of fecal or biopsy samples, which consistently identifies M. smithii as the dominant methanogen.^[38]^[39]^[23] Functionally, M. smithii plays a key role in interspecies hydrogen transfer within the gut, scavenging hydrogen gas (H₂) produced by fermentative bacteria such as Bacteroides species during carbohydrate breakdown. This hydrogenotrophy prevents H₂ accumulation, which could otherwise inhibit bacterial fermentation, thereby enhancing the efficiency of dietary polysaccharide degradation and short-chain fatty acid production. As a result, M. smithii is responsible for approximately 10-50% of the methane generated in the human gut, primarily through the reduction of CO₂ with H₂.^[40]^[23]^[26]^[41] The presence of M. smithii inversely correlates with overall bacterial diversity in the gut microbiome, with higher abundances linked to reduced microbial evenness, particularly among high-methane producers. Recent studies, including those from 2024-2025, have reinforced this association, suggesting that methanogenesis by M. smithii may alter fermentation dynamics and competitive interactions among bacteria.^[42]^[43] M. smithii is commonly detected and quantified using quantitative PCR (qPCR) targeting the methyl-coenzyme M reductase A (mcrA) gene, which is specific to methanogens and allows for precise measurement of its abundance relative to total microbiota. Additionally, breath methane levels serve as a non-invasive proxy for M. smithii activity, as elevated methane exhalation correlates with its metabolic output in the gut.^[44]^[45]^[46]

Microbial interactions

Symbiotic relationships

Methanobrevibacter smithii engages in mutualistic relationships with hydrogen-producing fermentative bacteria in the gut, such as Bacteroides thetaiotaomicron and various Clostridium species, by consuming excess H₂ and CO₂ generated during their fermentation of dietary polysaccharides. This interspecies hydrogen transfer relieves thermodynamic inhibition on bacterial metabolism, allowing fermenters to shift toward production of more reduced end products like acetate and propionate, which are beneficial short-chain fatty acids for the host.^[47] Similar positive associations have been observed with Clostridium clusters IV and XIVa, where M. smithii supports efficient carbohydrate breakdown by scavenging inhibitory gases.^[48] In cross-feeding dynamics, M. smithii indirectly supports acetogenic bacteria by modulating hydrogen availability, though it primarily competes with sulfate-reducing bacteria (SRB) for this substrate, potentially limiting SRB growth and sulfide production in the gut environment. This competition favors methanogenesis over sulfidogenesis, altering the redox balance and influencing community composition.^[40] While M. smithii does not directly produce formate or acetate in large quantities, its methanogenic activity can enhance overall substrate availability for acetogens through improved fermentation upstream.^[49] At the community level, M. smithii enhances fermentation efficiency by acting as a hydrogen sink, enabling more complete degradation of complex carbohydrates and increasing energy extraction from diet by up to 10-15% in modeled gut ecosystems.^[50] Antagonistic interactions arise from M. smithii's hydrogen depletion, which may inhibit growth of certain pathogens reliant on H₂ for energy. Additionally, extracellular vesicles (EVs) produced by M. smithii carry proteins and metabolites.^[51]^[52] In vitro syntrophic models further illustrate these dynamics; co-cultures of M. smithii with hydrogen producers like Christensenella minuta or B. thetaiotaomicron result in higher methane production compared to M. smithii alone, underscoring the dependence on bacterial partners for substrate supply and the mutual benefits of these consortia.^[53]^[41]

Associated viruses and phages

Methanobrevibacter smithii is primarily infected by temperate archaeal viruses, with the best-characterized being Methanobrevibacter smithii tailed virus 1 (MSTV1), a member of the proposed family Usuviridae. MSTV1 exhibits siphovirus morphology, featuring an icosahedral capsid approximately 65 nm in diameter and a long, non-contractile tail measuring about 270 nm. Its genome consists of double-stranded DNA, approximately 38.8 kb in length, with terminal redundancy that results in a circular configuration upon packaging.^[54] Infection by MSTV1 predominantly follows a lysogenic cycle, where the viral genome integrates into the host chromosome as a prophage, maintaining a stable coexistence with minimal impact on host growth rates. Spontaneous induction leads to low-level virion production, with only rare instances of assembly observed (e.g., in 1 out of 53 infected cells), suggesting a burst size of 1-5 virions per cell. In both in vitro cultures and human gut populations, the virus-to-host ratio remains low at approximately 0.1, reflecting controlled replication dynamics. While lytic cycles can occur, no significant induction events have been detected in vivo, supporting the dominance of lysogeny.^[54] Prophage regions of MSTV1 are integrated into the M. smithii genome, present in about 20% of analyzed strains, and carry auxiliary genes that may enhance host fitness. These include immunoglobulin-like domains in the major capsid protein, potentially aiding adhesion through programmed ribosomal frameshifting, as well as toxin-antitoxin systems that stabilize lysogeny. Metagenomic analyses have revealed additional tailed phages in the human gut virome targeting M. smithii.^[54]^[55] Ecologically, these viruses facilitate horizontal gene transfer among M. smithii populations, potentially disseminating adaptive traits like metabolic enhancements. The pseudomurein cell wall of M. smithii may serve as a partial barrier to phage adsorption, influencing infection specificity.^[54]^[55]

Implications for human health

Beneficial effects

Methanobrevibacter smithii contributes to enhanced energy harvest in the human gut by consuming hydrogen gas (H₂) produced during bacterial fermentation, thereby shifting microbial metabolism toward more efficient production of short-chain fatty acids (SCFAs), which can provide up to 10% of the host's daily caloric intake.^[23] This process aids host adaptation in nutrient-poor conditions, as evidenced by studies showing that colonization with M. smithii improves the breakdown of complex carbohydrates like fructans into absorbable metabolites.^[56] A meta-analysis of human fecal samples further links higher abundance of M. smithii to lower body mass index (BMI), suggesting a protective role against obesity through optimized energy balance.^[57] Through syntrophic interactions with fermentative bacteria such as Bacteroides thetaiotaomicron, M. smithii promotes elevated levels of SCFAs like butyrate and acetate by removing excess H₂, which otherwise inhibits further fermentation.^[26] These SCFAs nourish colonocytes, enhance gut barrier integrity, and exert anti-inflammatory effects by inhibiting pro-inflammatory pathways such as NF-κB signaling.^[34] Recent analyses confirm that methanogenic activity, dominated by M. smithii, correlates with increased SCFA production, supporting mucosal health and reducing systemic inflammation markers like C-reactive protein.^[58] In microbiomes with low bacterial diversity, M. smithii helps stabilize fermentation dynamics by scavenging H₂, preventing accumulation that could disrupt microbial communities and lead to inefficient nutrient processing.^[21] This stabilization is associated with improved outcomes in obesity management, where higher M. smithii levels correlate with better weight control and metabolic efficiency in dietary interventions.^[59] M. smithii exerts protective effects by competing with sulfate-reducing bacteria (SRBs) for H₂, thereby limiting hydrogen sulfide (H₂S) production, a toxic gas implicated in mucosal damage and inflammation.^[60] Methane, a byproduct of this process, influences gut motility by slowing transit times.^[61]

Disease associations

Methanobrevibacter smithii, a prominent methanogenic archaeon in the human gut and vaginal microbiomes, has been implicated in various disease states through its influence on microbial ecology, hydrogen metabolism, and host physiology. Its abundance or depletion correlates with conditions involving dysbiosis, such as obesity, severe acute malnutrition, constipation-predominant irritable bowel syndrome (IBS-C), colorectal cancer (CRC), and bacterial vaginosis (BV). These associations stem from metagenomic, qPCR, and clinical studies highlighting M. smithii's role in modulating fermentation efficiency and microbial interactions.^[23]^[21]^[62] In the context of obesity, M. smithii has been linked to increased energy harvest from diet, potentially contributing to adiposity. Metagenomic analyses of human gut microbiomes reveal that M. smithii enhances bacterial polysaccharide degradation by consuming hydrogen and formate, thereby boosting short-chain fatty acid production for host absorption. In gnotobiotic mouse models colonized with M. smithii alongside Bacteroides thetaiotaomicron, animals exhibited greater body fat accumulation compared to those with bacteria alone, supporting a mechanistic role in obesity development. Human studies further show higher M. smithii colonization in children associated with elevated weight z-scores, BMI z-scores, and overweight risk, with odds ratios indicating a positive correlation (OR = 2.5 for overweight). However, conflicting evidence exists; some metagenomic surveys report depletion of M. smithii in obese individuals compared to lean controls, suggesting context-dependent effects influenced by overall microbiota composition.^[23]^[39]^[63] Conversely, M. smithii depletion is observed in severe acute malnutrition, underscoring its potential protective role in nutrient utilization. In a large case-control study of Malian children, M. smithii was detected in only 4.2% of severely malnourished cases versus 40.9% of healthy controls, with significantly lower DNA copy numbers (mean 0.18 log₁₀ copies/ml vs. 1.99 log₁₀; p < 0.001). Detection absence strongly predicted malnutrition risk (OR = 0.06, 95% CI 0.02–0.15), and renutrition therapies failed to restore its levels, implying a disrupted colonization barrier in undernourished states. This pattern aligns with M. smithii's function in optimizing bacterial fermentation for energy extraction, which may be impaired in malnutrition.^[21] M. smithii is prominently associated with constipation-predominant IBS, where it predominates among methanogens and correlates with methane production that slows gut transit. In patients meeting Rome II criteria for IBS-C and positive for breath methane (>3 ppm), qPCR quantification of stool samples revealed M. smithii counts averaging 1.8 × 10⁷ copies/g—over 50-fold higher than in methane-negative IBS controls (3.2 × 10⁵ copies/g; p < 0.001)—comprising 7.1% of total bacteria versus 0.24% (p = 0.02). Breath methane levels strongly correlated with M. smithii abundance (R = 0.76, p < 0.001), establishing a threshold of 4.2 × 10⁵ copies/g for methane positivity. This predominance underscores M. smithii's contribution to delayed colonic motility via methane-mediated mechanisms.^[62] Elevated M. smithii levels are also observed in colorectal cancer, potentially exacerbating tumorigenesis through altered microbial networks. Multi-cohort metagenomic analysis across 11 populations (748 CRC cases, 471 adenomas, 882 controls) identified M. smithii as the most prevalent archaeon, enriched in 83% of CRC samples with significantly higher relative abundance than in healthy controls (mean 224.0 vs. 180.5; p = 0.0009). This progressive increase from controls to adenomas to CRC coincided with upregulated methanogenesis pathways (p = 0.047) and co-occurrences with oncogenic bacteria like Fusobacterium nucleatum, suggesting trophic interactions that may promote epithelial inflammation and permeability.^[64] In the vaginal microbiome, M. smithii detection serves as a marker for bacterial vaginosis, a common dysbiosis-linked infection. PCR and sequencing of vaginal samples from 33 BV-diagnosed women (Nugent score ≥7) detected M. smithii in 97% of cases (32/33), with 99% 16S rRNA gene similarity to reference strains and successful culture from 9/40 samples. In contrast, it was absent or rare in healthy controls, indicating high specificity for BV-associated shifts toward anaerobic overgrowth. These findings propose M. smithii as a diagnostic biomarker, complementing traditional Nugent scoring for improved laboratory confirmation.^[65] M. smithii has been implicated in rare invasive infections, including archaemia (archaeal bloodstream infection) and endocarditis, particularly in immunocompromised patients or those with bacteremia, often in co-infection with bacteria such as staphylococci or Enterobacteriaceae.^[66]

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[PDF] Methanobrevibacter smithii cell variants in human physiology and ...
Feb 27, 2025 · Methanobrevibacter smithii (M. smithii), initially isolated from human feces, has been recognised as a distinct taxon within the Archaea domain ...Missing: etymology | Show results with:etymology
[38]
Archaea in the Human Microbiome and Potential Effects on Human ...
In human feces, the most prevalent (>90%) archaeal representatives are Methanobrevibacter smithii and Candidatus Methanobrevibacter intestini, (1,4,5). In ...
[39]
Gut Colonization with Methanogenic Archaea Lowers Plasma ...
Oct 3, 2018 · It is known that certain members of methanogenic archaea (MA) could use methylated amines such as trimethylamine as growth substrates in culture ...Methanogenic Archaea (ma) · 16s Rrna-Based Taxonomic... · Results
[40]
Targeted isolation of Methanobrevibacter strains from fecal samples ...
Aug 31, 2024 · Methanobrevibacter species show a prevalence of more than 90% in fecal samples with an average abundance of 0.56% for Methanobrevibacter smithii ...
[41]
New insights from metagenomic survey and co-occurrence analysis
Jan 3, 2024 · In this study, the gut archaeome and bacteriome of 60 healthy adults from different region were analyzed by whole-genome shotgun sequencing.Missing: inverse | Show results with:inverse<|separator|>
[42]
Age-related dynamics of predominant methanogenic archaea in the ...
Apr 4, 2025 · It is known that Methanobrevibacter smithii, as the predominant archaeal species within the human gut, gradually becomes the dominant archaeal ...
[43]
Gut colonization with methanobrevibacter smithii is associated with ...
Presence and higher counts of M. smithii in the gut of children are associated with higher weight z-scores, higher BMI z-scores, and overweight.Missing: human | Show results with:human
[44]
Gut colonization with methanobrevibacter smithii is associated with ...
Nov 2, 2015 · smithii in the gut of children at school age is associated with overweight, higher weight, and BMI z-scores from 6 to 10 years of age. This ...Missing: early life
[45]
Hydrogen cross-feeders of the human gastrointestinal tract - PMC
Hydrogen plays a key role in many microbial metabolic pathways in the human gastrointestinal tract (GIT) that have an impact on human nutrition, health and ...
[46]
H2 generated by fermentation in the human gut microbiome ...
Jun 15, 2023 · By consuming H2, gut methanogenesis can decrease butyrate production. These shifts in butyrate production may also impact the competitive ...
[47]
Methane, Bacteria, Fungi, and Fermentation - MDPI
Methanogenic archaea, such as Methanobrevibacter smithii, have been associated with altered fermentation, decreased microbial diversity, and delayed intestinal ...
[48]
Multi-Cohort Analysis Reveals Altered Archaea in Colorectal Cancer ...
This study incorporated fecal metagenomic data from 10 independent cohorts across 7 countries and an additional in-house cohort, ...
[49]
Improved Quantitative Real-Time PCR Protocol for Detection ... - MDPI
Mar 5, 2023 · One of the methods of quantifying the prevalence of methanogens is quantitative real-time PCR (qPCR) of the methanogen-specific mcrA gene, and ...
[50]
Human methanogen diversity and incidence in healthy and ... - PMC
The mcrA gene was chosen as a proxy for methanogen detection as this gene can be readily amplified from Mbb. smithii (data not shown) which is the dominant ...Missing: qPCR | Show results with:qPCR
[51]
Methanobrevibacter smithii associates with colorectal cancer ...
Jun 12, 2025 · This provides the first mechanistic link between human gut archaeome and CRC and highlights its role in modulating health in humans through trophic control.
[52]
Metabolic Synergy between Human Symbionts Bacteroides and ...
May 10, 2022 · We studied the interactions between two dominant human gut microbes, B. thetaiotaomicron and M. smithii, using a seven-component culturing approach.<|control11|><|separator|>
[53]
Mutual Metabolic Interactions in Co-cultures of the Intestinal ...
Methanobrevibacter smithii is a major intestinal methanogenic archaeon, which ... thetaiotaomicron via the Wood–Ljungdahl pathway which in turn allowed B.
[54]
Metabolic niche of a prominent sulfate-reducing human gut bacterium
Our findings provide genetic and metabolic details of how this H 2 -consuming SRB shapes the responses of a microbiota to diet ingredients.
[55]
Community characteristics of the gut microbiomes of competitive ...
Aug 10, 2017 · smithii is positively associated with diets high in carbohydrates [22]. M. smithii increases the fermentation efficiency of many bacterial ...
[56]
Methane, Bacteria, Fungi, and Fermentation - PMC - NIH
Sep 2, 2025 · Furthermore, this altered fermentation dynamic contributes to the accumulation of short-chain fatty acids (SCFAs), which, in combination with ...
[57]
Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic ...
Aug 6, 2025 · Several major human pathogens use the large amounts of H 2 produced by colonic microbiota as an energy source for aerobic or anaerobic ...
[58]
Biogenesis of DNA-carrying extracellular vesicles by the dominant ...
Jun 3, 2025 · Here, we characterize EVs produced by Methanobrevibacter smithii, the dominant methanogenic archaeon in the human gut, which contains a ...Missing: quorum sensing
[59]
Syntrophy via Interspecies H2 Transfer between Christensenella ...
The most widespread and abundant of the gut methanogens, Methanobrevibacter smithii, produces CH4 from H2 and CO2, the products of bacterial fermentation of ...
[60]
Stable coexistence between an archaeal virus and the dominant ...
Sep 4, 2024 · Among them, Methanobrevibacter smithii stands out as the major component of the archaeal population in the GIT, accounting for over 90% of its ...
[61]
Phylogeny and disease associations of a widespread and ... - NIH
... Methanobrevibacter smithii and M. oralis which diverged 85,000 and 126,000 years ago, respectively. We hypothesize that these phages diversified in ...
[62]
Gut microbes' partnership helps body extract energy from food, store ...
Jun 12, 2006 · “The presence M. smithii improved the overall efficiency of the digestive system,” Gordon says. “It remains to be established whether we can ...
[63]
The Human Gut Microbiome: A Review of the Effect of Obesity and ...
One study showed that mice colonized with the archaeon Methanobrevibacter smithii had enhanced ability to metabolize fructans to short-chain fatty acids, ...
[64]
Meta-analysis comparing the frequency of Methanobrevibacter ...
These results suggest a genus-specific effect of human methanogenic Archaea on weight regulation and obesity and support that M. smithii is a neglected critical ...
[65]
Methanogenesis associated with altered microbial production of ...
In co-culture experiments, Bacteroides thetaiotaomicron expressed more glycoside hydrolases, enzymes that break down polysaccharides, and degraded and fermented ...
[66]
The gut microbiome in the fight against obesity: The potential of ...
Oct 16, 2023 · The gut microbiota is a novel target for obesity treatment. Dietary factors, including diet, prebiotics, and probiotics, may be important for ...
[67]
Methanogens and Hydrogen Sulfide Producing Bacteria Guide ...
Hydrogenotrophic methanogens such as M. smithii use H2 to generate CH4 (14,35), reducing H2 levels and increasing CH4 levels. The reduction in H2 in turn ...Missing: protective | Show results with:protective
[68]
Investigating the causal impact of gut microbiota on arthritis via ...
Nov 10, 2024 · Studies have shown that methane-producing archaea, such as Methanobrevibacter smithii, may affect immune responses by activating human ...
[69]
Establishing a causal link between gut microbes, body weight gain ...
Nov 13, 2015 · Also, probiotics are emerging as a potential therapy for metabolic syndrome. In fact, a handful of human studies and numerous animal studies ...
[70]
Beyond the Gut: Unveiling Methane's Role in Broader Physiological ...
Aug 26, 2025 · These include modulation of gastrointestinal motility, inflammation, short-chain fatty acid (SCFA) production, and energy regulation. Together, ...
[71]
Methanobrevibacter smithii Is the Predominant Methanogen in ...
May 10, 2012 · Among irritable bowel syndrome (IBS) patients, breath methane producers overwhelmingly have constipation predominance (C-IBS).
[72]
Obesity-associated gut microbiota is enriched in Lactobacillus ...
The gut microbiota associated with human obesity is depleted in M. smithii. Some Bifidobacterium or Lactobacillus species were associated with normal weight.
[73]
https://www.gastrojournal.org/article/S0016-5085(24
[74]
Detection of Methanobrevibacter smithii in vaginal samples ...
May 24, 2019 · These data suggest that the detection of M. smithii could be used as a biomarker for the laboratory diagnosis of bacterial vaginosis. Similar ...