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NT5E

NT5E is the official symbol for the gene encoding ecto-5'-nucleotidase, also known as CD73, a plasma membrane-bound enzyme that catalyzes the hydrolysis of extracellular adenosine 5'-monophosphate (AMP) to adenosine and inorganic phosphate.^[1] This glycoprotein, anchored via a glycosylphosphatidylinositol (GPI) linkage, exists as a dimer of approximately 70 kDa subunits and functions at neutral pH to regulate purinergic signaling by generating immunosuppressive adenosine from nucleotides like AMP.^[2] The gene is located on chromosome 6q14.3 and produces two protein isoforms through alternative splicing.^[1] CD73, the protein product of NT5E, plays a critical role in various physiological processes, including lymphocyte differentiation, where it serves as a marker, as well as in neuronal activity regulation and renal tubuloglomerular feedback.^[2] It is ubiquitously expressed across human tissues, with particularly high levels in the endometrium and ovary, and is localized to the plasma membrane, nucleoplasm, and cytosol.^[1] In the immune system, CD73 contributes to the extracellular conversion of AMP (derived from ATP) to adenosine, promoting adenosine-mediated suppression of inflammation and enhancement of angiogenesis.^[3] Mutations in NT5E are associated with calcification of joints and arteries (CALJA; also known as arterial calcification due to CD73 deficiency, ACDC), an autosomal recessive disorder characterized by calcium phosphate deposition in arteries and joints due to reduced enzymatic activity.^[2] Specific mutations, such as the nonsense variant S221X and missense variant C358Y, lead to near-total loss of CD73 function and have been linked to vascular and articular calcifications.^[2] Additionally, low NT5E expression is observed as a marker in certain immunodeficiencies.^[2] In oncology, NT5E overexpression is a notable feature in various cancers, particularly lung adenocarcinoma (LUAD), where it correlates with advanced tumor stages, nodal metastasis, and poor prognosis, including reduced overall and relapse-free survival.^[4] High CD73 expression in LUAD promotes immune evasion by fostering an immunosuppressive tumor microenvironment, with positive associations to infiltrating immune cells like macrophages and T cells, as well as checkpoint molecules such as PD-1 and CTLA-4.^[4] As a result, NT5E has emerged as a promising prognostic biomarker and therapeutic target, with inhibitors like selumetinib showing potential in preclinical models.^[4]

Gene

Location and Structure

The NT5E gene is located on the long arm of human chromosome 6 at band q14.3, spanning positions 85,449,584 to 85,495,791 (approximately 85.45–85.50 Mb on GRCh38 assembly).^[5] Orthologs are found in other mammals, including the mouse (Mus musculus) on chromosome 9 at positions 88,209,250–88,254,145 (88.21–88.25 Mb on GRCm39) and the rat (Rattus norvegicus) on chromosome 8 at approximately 98.15–98.20 Mb, with rat transcripts showing 89% nucleotide identity to the human sequence.^[6]^[6] The gene spans about 46 kb and consists of 9 exons, with the primary transcript (ENST00000257770) encoding a 574-amino-acid protein (isoform 1, UniProt P21589).^[5]^[7] Alternative splicing produces multiple isoforms, including a shorter variant lacking exon 7 that results in a 524-amino-acid protein.^[8] NT5E exhibits strong evolutionary conservation across vertebrates, reflecting its essential role in nucleotide metabolism, with orthologs identified in over 249 species via sequence similarity.^[9] Common polymorphisms, such as the c.1126A>G variant in exon 6 (rs2229523), have been associated with increased risk of aortic valve calcification, while rare loss-of-function mutations (e.g., p.Ser221Ter) underlie hereditary arterial and articular multiple calcification syndrome (ACDC; MIM 211800).^[10]^[11]^[12]^[2] The NT5E gene was first identified in the 1980s as encoding ecto-5'-nucleotidase, an enzyme serving as a marker for lymphocyte differentiation, particularly in B-cell maturation.^[2]

Expression Regulation

The promoter region of the NT5E gene harbors binding sites for transcription factors SP-1, AP-2, and SMAD proteins, along with cAMP-responsive elements, which collectively govern its basal transcriptional activity across various cell types.^[13] These elements facilitate interactions with SMAD2/3/4/5 and SP-1, as demonstrated by chromatin immunoprecipitation in hepatic stellate cells, where they drive inducible expression in response to profibrotic signals.^[14] Epigenetic mechanisms further modulate NT5E expression through promoter methylation and histone modifications. Hypermethylation of the NT5E CpG island in the 5' regulatory region silences transcription, as observed in approximately 42% of melanoma samples, where it correlates with reduced mRNA levels and lower metastatic potential to visceral sites.^[15] Conversely, in proximal tubule epithelial cells under diabetic conditions, TGF-β signaling promotes NT5E upregulation via demethylation and active histone marks, including increased H3K4me3 (trimethylation of histone H3 at lysine 4) and H3K9/14ac (acetylation at lysines 9/14), alongside reduced repressive marks H3K27me3 and H3K9me3, leading to a 16-fold transcript increase after 48 hours of high-glucose exposure.^[16] Post-transcriptional regulation occurs via microRNAs, with miR-30a-5p directly targeting the NT5E 3' untranslated region to suppress its expression, particularly in immune cells and non-small cell lung cancer contexts, where overexpression of miR-30a-5p reduces NT5E mRNA and protein levels by up to 70% in luciferase reporter assays.^[17] NT5E exhibits tissue-specific expression, with elevated levels in the liver, gastrointestinal tract, vascular endothelium, and female reproductive tissues, reflecting zonal patterns influenced by local microenvironments. Stimulus-induced upregulation, such as by hypoxia-inducible factor 1α (HIF-1α) binding to hypoxia response elements in the promoter, enhances NT5E transcription in hypoxic tumors and inflamed tissues, promoting adaptive adenosine production that can contribute to immunosuppressive outcomes in the immune microenvironment.^[18]

Protein

Structure and Localization

The protein encoded by the NT5E gene, known as CD73 or ecto-5'-nucleotidase, is a glycosylphosphatidylinositol (GPI)-anchored ectoenzyme that functions as a homodimer composed of two approximately 70-kDa subunits.^[19] Each subunit contains a catalytic domain that binds two zinc ions essential for its enzymatic function.^[20] Structurally, CD73 features an extracellular region divided into an N-terminal domain (residues 27–317) housing the zinc-binding active site and a C-terminal domain (residues 318–547) that facilitates dimerization and connects to the plasma membrane via a GPI anchor at Ser-549.^[20] Unlike transmembrane proteins, the GPI anchor attaches CD73 to the outer leaflet of the lipid bilayer without a cytoplasmic tail, enabling its localization in membrane lipid rafts.^[7] CD73 is predominantly localized to the plasma membrane of endothelial cells, subsets of lymphocytes such as regulatory T cells, and various tumor cells, where it contributes to extracellular nucleotide metabolism.^[21] A soluble form of CD73 can be released into the extracellular space through proteolytic cleavage or phospholipase-mediated hydrolysis of the GPI anchor, allowing it to exert effects remotely from the cell surface.^[22] Post-translational modifications, particularly N-linked glycosylation at four consensus sites (Asn53, Asn311, Asn333, and Asn403), play a key role in CD73's folding, stability, and activity, with alterations in glycosylation patterns potentially modulating its membrane retention and function.^[23]

Enzymatic Activity

CD73, encoded by the NT5E gene, functions as an ecto-5'-nucleotidase that catalyzes the hydrolysis of extracellular 5'-adenosine monophosphate (AMP) to adenosine and inorganic phosphate (Pi). This enzymatic reaction is the primary catalytic activity of CD73, exhibiting Michaelis-Menten kinetics with a Km value for AMP of approximately 10.5 μM, consistent with a range of 1–50 μM reported in biochemical assays using purified human enzyme.^[24] In the broader context of extracellular nucleotide metabolism, CD73 represents the terminal enzyme in the ecto-nucleotidase cascade, where CD39 (ecto-nucleoside triphosphate diphosphohydrolase-1) first hydrolyzes adenosine triphosphate (ATP) or adenosine diphosphate (ADP) to AMP, which CD73 then converts to adenosine. This sequential process is crucial for generating extracellular adenosine from pro-inflammatory ATP. The specific reaction mediated by CD73 is:

\text{AMP} \rightarrow \text{Adenosine} + \text{P}_\text{i}

CD73 enzymatic activity is dependent on divalent metal cofactors, particularly Zn²⁺ ions bound at the active site in the N-terminal domain, where two zinc ions coordinate the substrate and facilitate phosphohydrolase catalysis. The enzyme exhibits optimal activity at a neutral pH around 7.5, aligning with physiological extracellular conditions.^[25]^[26] CD73 is subject to inhibition by both natural and synthetic compounds, modulating its catalytic efficiency. ATP acts as a competitive inhibitor, binding to the active site and preventing AMP hydrolysis with kinetics that reflect its structural similarity to the substrate. Synthetic inhibitors, such as α,β-methylene-ADP (AMPCP), a non-hydrolyzable AMP analog, potently block CD73 activity through competitive binding, exhibiting a Ki value of 88 nM in human enzyme assays. These inhibitors demonstrate high specificity and have been instrumental in dissecting CD73's role in nucleotide metabolism.^[27]^[25]

Biological Functions

In Immune System

NT5E, encoding the ecto-5'-nucleotidase CD73, plays a central role in immune suppression by generating extracellular adenosine, which binds to A2A adenosine receptors on T cells, thereby inhibiting effector T-cell proliferation and cytokine production while promoting the expansion and suppressive function of regulatory T cells (Tregs).^[28] This mechanism dampens adaptive immune responses, maintaining immune homeostasis and preventing excessive activation that could lead to autoimmunity.^[29] CD73 is prominently expressed on the surface of regulatory T cells and antigen-presenting cells, such as dendritic cells and monocytes, where it contributes to local adenosine accumulation that suppresses proinflammatory signaling.^[30] In experimental models of inflammation, CD73 deficiency results in heightened immune responses, including increased T-cell expansion, elevated interferon-γ and interleukin-6 production, and exacerbated tissue damage, underscoring its anti-inflammatory role in vivo.^[31] CD73 cooperates closely with CD39, another ectonucleotidase, in the ATP-adenosine pathway: CD39 hydrolyzes ATP to AMP, which CD73 then converts to adenosine, amplifying immunosuppressive effects across immune subsets.^[28] Recent Mendelian randomization analyses have further revealed NT5E's causal involvement in Reg3β-induced M2 macrophage polarization, where elevated NT5E expression in stimulated macrophages enhances anti-inflammatory M2 phenotypes, mediated through interactions with plasma proteins and influencing immune cell dynamics in conditions like myocarditis.^[32] As a marker of T-cell differentiation, CD73 exhibits low expression on immature or naive T cells but is upregulated in mature effector memory subsets, such as central and effector memory CD4+ and CD8+ T cells, correlating with their enhanced suppressive or functional capabilities.^[33] This pattern of expression highlights CD73's association with advanced T-cell maturation stages in peripheral lymphoid tissues.^[30]

In Cardiovascular System

In the cardiovascular system, CD73 (encoded by NT5E) plays a critical role in maintaining endothelial barrier integrity through the generation of extracellular adenosine, which stabilizes vascular permeability by activating A2B adenosine receptors on endothelial cells.^[34] This adenosine-mediated signaling enhances endothelial cell junctional complexes, reducing paracellular leakage during inflammatory or stress conditions, as demonstrated in studies where CD73 inhibition leads to increased vascular permeability.^[35] Additionally, CD73-derived adenosine at the endothelium-platelet interface exerts anti-thrombotic effects by inhibiting platelet activation and aggregation, thereby preventing thrombus formation and supporting vascular homeostasis.^[36] These functions highlight CD73's contribution to non-immune vascular protection, distinct from its roles in lymphoid modulation.^[37] In vascular smooth muscle cells (SMCs), CD73 modulates transforming growth factor-β (TGFβ) signaling via adenosine production, which dampens profibrotic responses. Studies using Nt5e knockout in SMCs have shown elevated TGFβ-induced Smad2/3 phosphorylation and downstream extracellular matrix gene expression, resulting in enhanced fibrotic potential compared to wild-type cells.^[38] This regulatory mechanism underscores CD73's role in preventing excessive SMC remodeling and fibrosis in the vessel wall.^[39] Normal CD73 expression inhibits vascular and periarticular calcification by promoting adenosine signaling that suppresses hydroxyapatite crystal deposition. Activation of A2A adenosine receptors by CD73-generated adenosine restrains osteogenic differentiation in arterial SMCs and reduces alkaline phosphatase activity, thereby limiting calcium phosphate accumulation in arteries and joints.^[40] This protective effect is evident from observations where CD73 deficiency leads to ectopic mineralization, confirming its inhibitory function under physiological conditions.^[41] During ischemic conditions, CD73 is upregulated in endothelial and vascular cells via hypoxia-inducible factor-1α (HIF-1α), enhancing adenosine production to protect against reperfusion injury. This adaptive response stabilizes post-ischemic endothelial barrier function and attenuates tissue damage by counteracting oxidative stress and inflammation through A2B receptor activation.^[42] Experimental models of intestinal and cardiac ischemia-reperfusion have demonstrated that CD73-mediated adenosine generation is essential for this cytoprotective mechanism.^[43]

In Nervous System

CD73 regulates neuronal activity by hydrolyzing AMP to adenosine, which modulates synaptic transmission and nociception. In the central nervous system, CD73-derived adenosine inhibits pain signaling by activating A1 adenosine receptors on sensory neurons, reducing nociceptive responses in models of inflammatory and neuropathic pain.^[44] Additionally, CD73 contributes to adenosine formation from synaptic ATP release, influencing mood, memory, and sleep-wake cycles through non-canonical pathways independent of extracellular adenosine levels in some contexts.^[45]^[46]

In Renal System

In the kidney, CD73 is essential for tubuloglomerular feedback (TGF), a mechanism that regulates glomerular filtration rate (GFR) in response to changes in tubular fluid NaCl concentration at the macula densa. CD73 generates adenosine from AMP, which acts on A1 adenosine receptors on afferent arterioles to induce vasoconstriction and adjust GFR. Studies in Cd73-deficient mice demonstrate impaired TGF responsiveness, with reduced changes in stop-flow pressure and superficial nephron GFR, highlighting CD73's critical role in renal autoregulation.^[47]^[48]

Clinical Associations

Genetic Deficiencies

Genetic deficiencies in the NT5E gene, which encodes the enzyme ecto-5'-nucleotidase (CD73), lead to rare monogenic disorders characterized by loss of function and impaired extracellular adenosine generation. The most well-established condition is calcification of joints and arteries (CALJA, also known as arterial calcification due to CD73 deficiency or ACDC; MIM 211800), an autosomal recessive disorder first described in 2011. This adult-onset syndrome typically manifests in the second or third decade of life with progressive, painful ectopic calcifications in the joints of the hands and feet, as well as in lower extremity arteries, leading to ischemia, skin ulcers, and reduced mobility. Biallelic loss-of-function mutations, such as the homozygous missense variant p.Cys358Tyr or nonsense variant p.Ser221*, abolish CD73 enzymatic activity, resulting in deficient production of adenosine, a key regulator of mineralization, and subsequent dysregulation of pyrophosphate metabolism that promotes vascular and periarticular calcification.^[12]^[49] NT5E deficiencies have also been linked to immunodeficiencies through disrupted adenosine-mediated immune regulation, as CD73 is a marker of lymphocyte differentiation and contributes to immunosuppressive signaling. In various primary immunodeficiency diseases, such as adenosine deaminase (ADA) deficiency (MIM 102700), reduced NT5E expression impairs T-cell function and leads to combined immunodeficiency phenotypes resembling severe combined immunodeficiency (SCID), with recurrent infections and lymphopenia due to altered purine metabolism and adenosine accumulation toxicity.^[2] The phenotypic spectrum of NT5E variants includes a range from severe biallelic loss-of-function mutations causing full CALJA to hypomorphic alleles, such as compound heterozygous frameshift and missense variants, which may result in milder vascular and joint calcifications with variable onset and severity. While some hypomorphic variants can overlap with subtle immune dysregulation, such as mild lymphoproliferation, the predominant effects remain focused on ectopic calcifications in arteries and joints, without consistent progression to severe systemic autoimmunity.^[12]^[49] Diagnosis of NT5E-related deficiencies relies on genetic sequencing to identify biallelic loss-of-function variants in NT5E, confirmed by functional assays demonstrating enzyme activity less than 10% of normal levels in patient-derived cells or plasma. Clinical evaluation includes radiographic imaging to document calcifications, alongside exclusion of secondary causes like chronic kidney disease, with prevalence estimated at less than 1 in 1,000,000 based on fewer than 25 reported cases worldwide.^[12]^[49]^[50]

Autoimmune Diseases

In systemic lupus erythematosus (SLE), NT5E expression is significantly downregulated in regulatory T cells (Tregs), particularly CD4+ IL2RA+ FOXP3+ Tregs, compared to healthy controls. This reduction impairs the immunosuppressive function of Tregs by limiting adenosine production through the ectonucleotidase pathway, thereby failing to adequately suppress effector T cell proliferation and contributing to unchecked B-cell activation and autoantibody production. Studies have shown that this dysregulation is linked to hypermethylation of NT5E promoter regions in SLE Tregs, further exacerbating the loss of suppressive capacity.^[51] Recent genetic analyses have identified NT5E polymorphisms, such as the rs2229524 variant (p.Met379Thr), associated with SLE susceptibility, particularly in individuals of European ancestry, where the minor allele correlates with decreased NT5E transcript levels in peripheral blood mononuclear cells (PBMCs). This variant alters enzymatic activity, reducing adenosine-mediated immune regulation and promoting inflammatory responses. While specific odds ratios vary across studies, the association highlights NT5E's role in polygenic risk for SLE.^[52] In other rheumatologic diseases like rheumatoid arthritis (RA), NT5E dysregulation manifests as reduced CD73 expression on synovial fluid mononuclear cells (SFMCs), including CD8+ T cells and B cells, leading to diminished adenosine generation and heightened inflammation. Although cellular CD73 is decreased, soluble forms of CD73 derived from extracellular vesicles can contribute to paracrine adenosine signaling; however, overall pathway imbalance favors pro-inflammatory states due to insufficient anti-inflammatory adenosine in the synovial microenvironment. This altered expression inversely correlates with disease activity scores, such as DAS28, underscoring NT5E's contribution to joint pathology.^[53]^[54] Mechanistically, defects in the NT5E-adenosine deaminase (ADA) axis in lupus models amplify autoantibody production by disrupting adenosine homeostasis; elevated ADA activity degrades protective adenosine, while NT5E deficiency limits its synthesis, resulting in enhanced TH17 polarization and reduced Treg-mediated suppression. In experimental SLE models, CD73 knockout exacerbates autoimmunity and vascular dysfunction, with increased autoantibody levels and inflammatory cytokine production. Pharmacological restoration of this axis, such as through A2A receptor agonists, partially reverses these effects by bolstering Treg function.^[55]^[56] Epidemiological data indicate that NT5E variants contribute to increased SLE risk across diverse populations, including Asian cohorts where GWAS have identified immune-related loci influencing disease susceptibility, though NT5E-specific effects require further validation in large-scale studies. In East Asian populations, polygenic risk scores incorporating such variants highlight heightened SLE severity, potentially due to environmental interactions amplifying NT5E dysregulation. These findings support NT5E as a modifier in multi-ethnic autoimmune risk profiles.^[57]^[58]

Oncology

NT5E, encoding the enzyme CD73, is frequently overexpressed in various solid tumors, contributing to tumor progression and poor clinical outcomes. Pan-cancer analyses have revealed upregulation of NT5E in more than 50% of solid malignancies, including breast invasive carcinoma, prostate adenocarcinoma, and hepatocellular carcinoma (HCC).^[59]^[4] In breast and prostate cancers, elevated NT5E levels correlate with enhanced tumor angiogenesis and metastasis in preclinical models.^[60] A 2025 study on HCC demonstrated that NT5E expression is transcriptionally driven by the oncogene C20orf27, which promotes HCC cell proliferation, migration, and metastasis through activation of cell cycle regulators such as MDM2, PCNA, Cyclin E1, and CDK2, as evidenced by RNA sequencing, immunohistochemistry on 144 patient samples, and in vivo tumor growth assays showing reduced progression upon NT5E knockdown.^[61] In the tumor microenvironment (TME), CD73 expressed on cancer-associated fibroblasts (CAFs) plays a pivotal role in facilitating epithelial-mesenchymal transition (EMT) and immune evasion. CAFs, comprising up to 90% of CD73-positive cells in colorectal cancer TME, generate immunosuppressive adenosine via an A2B receptor-mediated feedforward circuit, which upregulates CD73 and suppresses antitumor immunity, as shown in patient specimens and mouse models where CD73 neutralization reduced EMT markers and tumor growth.^[62] Pan-cancer analyses from 2022 to 2025 further link high NT5E expression in CAFs to upregulated EMT signatures in cancers such as basal cell carcinoma, head and neck squamous cell carcinoma, pancreatic adenocarcinoma, and skin cutaneous melanoma, correlating with increased CAF and endothelial cell infiltration that fosters an immunosuppressive milieu.^[63] These studies associate NT5E overexpression with adverse prognosis across multiple malignancies, with hazard ratios (HR) for overall survival ranging from 1.5 to 2.0 in head and neck squamous cell carcinoma (HR=1.51), stomach adenocarcinoma (HR=1.84), and cervical squamous cell carcinoma (HR=1.74).^[63]^[4] Recent multi-omics investigations, including bulk transcriptomics and single-cell RNA sequencing in pancreatic ductal adenocarcinoma, implicate NT5E in polyamine metabolism pathways that drive tumor progression and immune dysregulation. NT5E, identified as a core regulator, is highly expressed in epithelial and mesenchymal tumor cells as well as CD73-positive CAFs, where its knockdown disrupts polyamine synthesis, impairs proliferation and migration, and alters TME composition to reduce immunosuppression.^[64] Additionally, 2025 analyses highlight NT5E's involvement in efferocytosis, the process of apoptotic cell clearance that promotes tumor tolerance and metastasis, further linking it to aggressive disease phenotypes.^[65] NT5E exerts a paradoxical influence on anti-tumor immunity, primarily by suppressing CD8+ T cell activation through adenosine-mediated inhibition, which dampens effector functions and promotes T cell exhaustion in the TME. High NT5E levels correlate with reduced CD8+ T cell infiltration and impaired antitumor responses in solid tumors, as observed in preclinical models where CD73 blockade restored T cell-mediated tumor control.^[66] Targeting NT5E via microRNAs offers a strategy to reinvigorate immunity; for instance, miRNAs such as the miR-30 family downregulate CD73 expression, enhancing CD8+ T cell proliferation and antitumor efficacy in colorectal cancer models by elevating extracellular ATP and reducing adenosine suppression.^[67] This miRNA-mediated approach has shown potential to modulate the TME and improve immune checkpoint therapy outcomes without directly addressing therapeutic development.^[60]

Therapeutic Targeting

Inhibitor Development

Development of inhibitors targeting NT5E (CD73) began in the early 2010s, with initial efforts focusing on nucleotide-based scaffolds like α,β-methylene-ADP (AMPCP) as a lead compound for enzymatic blockade.^[68] By the mid-2010s, this progressed to more selective small-molecule and antibody-based agents, driven by recognition of CD73's role in adenosine-mediated immunosuppression in tumors.^[69] As of 2025, no CD73 inhibitors have received FDA approval as monotherapies, but several are advancing in combination regimens, with pending approvals anticipated for immuno-oncology combos based on phase II data.^[70] Prominent examples include oleclumab (MEDI9447), an anti-CD73 monoclonal antibody (mAb) developed by AstraZeneca, and CPI-006 (mupadolimab), a humanized anti-CD73 mAb from Corvus Pharmaceuticals. Oleclumab is under evaluation in multiple phase II trials, often combined with PD-1 inhibitors like durvalumab for solid tumors such as non-small cell lung cancer (NSCLC) and triple-negative breast cancer (TNBC); for instance, the NeoCOAST-2 trial reported improved major pathological response rates in neoadjuvant settings for resectable NSCLC as of May 2025.^[71] Similarly, CPI-006 is in phase I/II studies, including combinations with pembrolizumab for advanced solid tumors, where it has shown preliminary efficacy in reducing tumor-associated B-cell counts and enhancing immune activation in 34 patients with refractory cancers.^[72] These agents exemplify the shift toward antibody formats for durable target engagement, though small-molecule inhibitors like quemliclustat (AB680) are also progressing in parallel phase I/II trials for similar indications, including receipt of FDA orphan drug designation for pancreatic cancer in July 2025.^[70]^[73] The primary mechanism of these inhibitors involves competitive blockade of CD73's ecto-5'-nucleotidase activity, which prevents the conversion of AMP to immunosuppressive adenosine in the tumor microenvironment (TME), thereby reducing adenosine levels in preclinical models and promoting T-cell infiltration and activation.^[74] This enhances anti-tumor immunity, with synergistic effects observed when combined with chemotherapy in preclinical and clinical settings. Key challenges include resistance mechanisms arising from alternative adenosine sources, such as upregulated CD39 activity or intracellular nucleotidases, which can bypass CD73 inhibition and sustain TME suppression.^[75] Advances in 2025 address this through dual-targeting strategies, including NT5E-polyamine inhibition for pancreatic ductal adenocarcinoma (PDAC); recent studies highlight how polyamine metabolism sustains PDAC progression via adenosine pathways, proposing combined blockade to disrupt tumor ecosystem dynamics and improve immunotherapy responses in preclinical PDAC models.^[76] Overall, 18 CD73 inhibitors remain in clinical development as of March 2025, emphasizing combo therapies to overcome these hurdles.^[70]

Diagnostic Biomarkers

High expression of NT5E, encoding the CD73 enzyme, serves as a prognostic biomarker across multiple cancer types, with elevated levels consistently linked to poorer patient outcomes in large-scale analyses. In pan-cancer evaluations using The Cancer Genome Atlas (TCGA) data, high NT5E expression correlates with reduced overall survival in at least nine solid tumor types, including head and neck squamous cell carcinoma, lung adenocarcinoma, and stomach adenocarcinoma.^[77] A 2025 study further highlighted this association in the context of efferocytosis, where NT5E upregulation in lung adenocarcinoma promotes immune evasion and worsens prognosis through enhanced tumor-associated macrophage infiltration.^[4] Soluble CD73 detected in serum represents a non-invasive liquid biopsy marker for predicting immunotherapy response, particularly in metastatic melanoma and other immunogenic cancers. Elevated serum levels of soluble CD73 enzyme activity are significantly associated with inferior overall survival and progression-free survival in patients undergoing checkpoint inhibitor therapy.^[78] High circulating CD73 concentrations also indicate resistance to anti-PD-1/PD-L1 treatments by fostering an immunosuppressive microenvironment, with studies reporting worse outcomes in patients with levels exceeding typical baselines, though specific thresholds vary by assay.^[79] In hepatocellular carcinoma (HCC), NT5E expression has emerged as a valuable biomarker for risk stratification, with recent cohort studies demonstrating its correlation with aggressive disease progression and poor prognosis. A 2025 analysis of HCC tumor microenvironments underscored CD73's role in modulating immune responses, where high expression in malignant cells and associated fibroblasts predicts reduced survival and immunotherapy efficacy.^[80] For macrophage-related cancers, such as those involving tumor-associated macrophages (TAMs), NT5E integrates with polyamine metabolism pathways to enhance biomarker utility; multi-omics profiling reveals that CD73-positive fibroblasts near tumor cells drive polyamine-driven immunosuppression, linking elevated NT5E to macrophage polarization and immune evasion in breast and other solid tumors.^[81]^[82]

References

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[34]
CD73/Ecto-5′-Nucleotidase Protects Against Vascular ...
Apr 24, 2006 · Neutrophil-derived 5′-adenosine monophosphate promotes endothelial barrier function via CD73-mediated conversion to adenosine and endothelial ...
[35]
VAP-1 and CD73, Endothelial Cell Surface Enzymes in Leukocyte ...
Oct 25, 2007 · Here we briefly review the role of vascular adhesion protein-1 (VAP-1) and CD73, 2 cell surface enzymes, in leukocyte migration form the blood into the tissues.
[36]
https://www.ahajournals.org/doi/10.1161/circulationaha.105.595249
[37]
Ecto-5'-nucleotidase (Nt5e/CD73)-mediated adenosine signaling ...
Feb 1, 2023 · Our study investigates whether CD73-derived adenosine modifies TGFβ signaling in vascular smooth muscle cells (SMCs).Missing: knockout fibrosis
[38]
Biomolecules Orchestrating Cardiovascular Calcification - PMC
Arterial calcification consists of calcium phosphate deposits in the forms of hydroxyapatite ... They found that CD73 inhibits calcification by activating ...<|control11|><|separator|>
[39]
Genetics in Arterial Calcification | Circulation Research
Aug 19, 2011 · The authors detected biallelic nonsense, missense, and single-nucleotide insertion-frameshift mutations in the ecto-5′-nucleotidase gene NT5E, ...
[40]
Nucleotidase (CD73) and the A2B Adenosine Receptor - PMC
Hypoxia-Inducible Factor-1α–Dependent Protection from Intestinal Ischemia/Reperfusion Injury Involves Ecto-5′-Nucleotidase (CD73) and the A2B Adenosine Receptor.
[41]
Endogenous adenosine produced during hypoxia attenuates ...
To test this hypothesis, endothelial cells were pre-exposed to conditions of hypoxia, which induce ecto-nucleotidase (CD39 and CD73) activity (20 mm Hg, 48 ...
[42]
211800 - CALCIFICATION OF JOINTS AND ARTERIES; CALJA
Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands (St. Hilaire et al., ...
[43]
Calcification of Joints and Arteries (CALJA) Is a Rare Cause of ...
May 18, 2023 · CALJA is a rare disease that leads to chronic arthritis and lower limb claudication due to hydroxyapatite crystal deposition.
[44]
AHR/TET2/NT5E axis downregulation is associated with the risk of ...
Oct 31, 2022 · Furthermore, NT5E expression was significantly downregulated in Tregs of patients with SLE, which can be attributed to the dysregulation of NT5E ...
[45]
Altered Expression of Ecto-5'-Nucleotidase (NT5E) in SLE Patients ...
The p.Met379Thr (rs2229524 T to C) substitution in the NT5E gene has been linked to systemic lupus erythematosus (SLE) in individuals of European ancestry,1 ...Missing: polymorphisms susceptibility<|separator|>
[46]
Altered CD39 and CD73 Expression in Rheumatoid Arthritis - NIH
Dec 19, 2023 · Given the role of CD73 in adenosine production and the established correlation between CD73 expression in PB and synovial fluid [28], it is ...
[47]
Correlation of Low CD73 Expression on Synovial Lymphocytes With ...
Nov 21, 2014 · The decreased CD73 expression on SFMCs, in turn, results in reduced adenosine production, which leads to a decreased potential for ...
[48]
Ectonucleotidase-Mediated Suppression of Lupus Autoimmunity ...
[The expression of CD73 in CD4+ regulatory T cells in patients with new-onset systemic lupus erythematosus]. ... function of T regulatory cells to mitigate ...
[49]
The roles of adenosine signaling in systemic lupus erythematosus
Adenosine is a critical factor for human immune homeostasis, which acts as an immunosuppressive signal and can prevent the hyperactivity of human immune system.
[50]
Genome-Wide Association Study in Asian Populations Identifies ...
Feb 12, 2010 · SNPs showed significant association with SLE in a joint analysis of four independent Asian cohorts. SNP, Chr, Nearby Gene, Location relative to ...
[51]
Meta-analysis of 208370 East Asians identifies 113 susceptibility ...
This study identified 113 genomic regions including 46 novel loci for SLE risk. The study prioritised 110 putative causal variants including 10 putative causal ...Missing: NT5E | Show results with:NT5E
[52]
Comprehensive evaluation of NT5E/CD73 expression and its ...
Mar 7, 2018 · The results further suggested that CD73 highly expressed in most kinds of cancers including bladder, brain, invasive lobular breast, esophageal, ...
[53]
Transcription Factors and MicroRNAs Regulating CD73/NT5E
Besides expression of the full-length molecule NT5EL (NT5E-201, 574 aa), a spliced version lacking exon 7 designated NT5ES (NT5E-203, 525 aa) can be detected ...
[54]
C20orf27 promotes hepatocellular carcinoma progression via NT5E
Jul 21, 2025 · Previous studies have shown that NT5E drives HCC progression and metastasis. Given NT5E's important role in HCC, 144 HCC patients were ...
[55]
CD73 on cancer-associated fibroblasts enhanced by the A 2B - Nature
Jan 24, 2020 · CD73, an ecto-5′-nucleotidase (NT5E), serves as an immune checkpoint by generating adenosine (ADO), which suppresses immune activation ...
[56]
Pan-cancer analysis identifies NT5E as a novel prognostic ...
NT5E could serve as a potential prognostic biomarker for cancers. The potential mechanism may be related to the upregulated EMT function of CAFs.
[57]
Multi-omics analysis of polyamine metabolism implicates NT5E ...
Polyamine metabolism is upregulated in PDAC and linked to poor prognosis. NT5E is a key regulator, and its inhibition may suppress tumor progression.Missing: efferocytosis | Show results with:efferocytosis
[58]
NT5E (CD73) as a prognostic biomarker and therapeutic target ...
Feb 5, 2025 · NT5E, an ecto-5'-nucleotidase enzyme, has been implicated in cancer progression, particularly in efferocytosis.
[59]
Targeting CD73 increases therapeutic response to immunogenic ...
Mar 7, 2023 · Taken together, these findings indicate that CD73 may inhibit anti-tumor immunity to decrease the therapeutic efficacy of chemotherapy, which ...
[60]
Elevated ATP via enhanced miRNA-30b, 30c, and 30e ...
Mar 24, 2022 · ... miRNA-30c-e downregulates CD73 expression in CD8+ T cells. Results ... cell proliferation and tumor growth of colorectal cancer by targeting CD73.
[61]
Development of CD73 Inhibitors in Tumor Immunotherapy and ...
AMPCP has served as a foundational scaffold for the development of CD73 inhibitors, as originally proposed by the Müller group, leading to numerous derivatives ...
[62]
An Exceptionally Potent Inhibitor of Human CD73 - ACS Publications
Jul 23, 2019 · We found AB680 to be a reversible, slow-onset competitive inhibitor of human CD73 with a K i of 5 pM. Clinical candidates of this potency are uncommon.
[63]
Development of CD73 Inhibitors in Tumor Immunotherapy and ...
Mar 19, 2025 · Currently, 18 CD73 inhibitors are in clinical trials, showing promising results in combination therapy for various solid tumors.
[64]
the platform phase 2 NeoCOAST-2 trial | Nature Medicine
May 31, 2025 · In the phase II NeoCOAST study, neoadjuvant durvalumab combined with oleclumab or monalizumab led to numerical improvement in mPR rates and ...<|separator|>
[65]
CD73: a new immune checkpoint for leukemia treatment - Frontiers
Mar 5, 2025 · CD73 exhibits diverse forms and functions through post-translational protein modifications; it specifically undergoes two main modifications, ...
[66]
Inhibition of the Adenosine Pathway to Potentiate Cancer ...
Sep 9, 2020 · We provide an overview of the current status of clinical trials targeting the adenosine pathway, including CD73, CD39, and adenosine receptors.
[67]
https://pmc.ncbi.nlm.nih.gov/articles/PMC8947394/
[68]
CD39 and CD73: biological functions, diseases and therapy
Nov 5, 2025 · Furthermore, CD73-mediated AMP hydrolysis requires divalent cations, particularly Mg2+, and exhibits optimal activity at pH 9.5 within the range ...
[69]
Efflux of N1-acetylspermidine from hepatoma fosters macrophage ...
Jun 10, 2025 · 36–41 In this study, we identified polyamine metabolism as the predominant metabolic pathway enriched in HCC tumors. N1-acetylspermidine (N1-.
[70]
768P Investigation of CD73 expression in response to ...
Results: In TCGA cohort, higher CD73/NT5E expression was associated with worse prognosis in 9 types of solid tumors, including HNSC, UVM, TGCT, STAD, LUAD, LUSC ...
[71]
Soluble CD73 as biomarker in patients with metastatic melanoma ...
Dec 4, 2017 · Our results show that high levels of sCD73 enzyme activity were significantly associated with poor overall survival and progression-free survival in patients ...
[72]
Exosomal CD73 from serum of patients with melanoma suppresses ...
Mar 10, 2022 · Elevated levels of circulating CD73 in patients with cancer have been associated with disease progression and poor response to immunotherapy.Missing: threshold | Show results with:threshold
[73]
Research progress of CD73-adenosine signaling regulating ...
May 26, 2025 · In this article, we review recent progress in the immunotherapy of HCC by targeting CD73-adenosine signaling to regulate the TME of HCC.
[74]
Multi-omics analysis of polyamine metabolism implicates NT5E ...
Notably, CD73+ cancer-associated fibroblasts (CAFs) were enriched near tumor cells, suggesting their involvement in metabolic crosstalk and immunosuppression.
[75]
Cancer cell-derived arginine fuels polyamine biosynthesis in tumor ...
Jun 9, 2025 · Here, we reveal that the metabolic interplay between cancer cells and macrophages plays a dominant role in arginine-driven breast cancer progression.
[76]
Integrated multiomics machine learning and mediated Mendelian ...
Previous studies have found that NT5E plays a crucial role in immune evasion and drug resistance in cancer cells, whereas DUSP4 is involved in tumor cell ...
[77]
Exploring the impact of neutrophils on lung adenocarcinoma using ...
Jul 3, 2025 · We evaluated the association between immune cell characteristics and LUAD using Mendelian randomization (MR) analysis based on genome-wide ...