Myocarditis is an inflammatory disease of the myocardium, the muscular middle layer of the heart wall, characterized by infiltration of immune cells and potential myocyte damage that impairs cardiac contractility and electrical conduction.[1] It manifests across a spectrum from subclinical or self-limited episodes to acute fulminant presentations with cardiogenic shock, arrhythmias, or sudden cardiac death, with histological confirmation via endomyocardial biopsy revealing lymphocytic, eosinophilic, or giant cell patterns depending on etiology.[1][2]Viral pathogens, particularly enteroviruses like coxsackievirus B and adenoviruses, constitute the predominant cause through direct cytopathic effects or immune-mediated injury, though bacterial, parasitic, and fungal agents contribute less frequently.[3] Non-infectious triggers encompass autoimmune disorders such as systemic lupus erythematosus or giant cell myocarditis, hypersensitivity to drugs or toxins, and idiopathic forms potentially involving molecular mimicry.[3][4] In recent empirical observations, mRNA-based COVID-19 vaccines have been causally linked to elevated incidence rates, especially in adolescent and young adult males post-second dose, with population studies reporting risks up to several-fold above baseline, though clinical severity often proves milder than in viral cases.[5][6][7]Clinically, patients may present with chest pain, dyspnea, palpitations, or heart failure symptoms, alongside biomarkers like elevated troponin and inflammatory markers, while electrocardiographic abnormalities, echocardiographic wall motion issues, and cardiac MRI aid diagnosis.[1]Management centers on etiology-specific interventions where possible—such as antivirals or immunosuppression for autoimmune variants—supplemented by supportive care including inotropes, mechanical circulatory support, or heart transplantation in refractory cases, with long-term risks including dilated cardiomyopathy in 10-20% of survivors.[2] Despite its relative rarity (annual incidence approximating 10-20 per 100,000), myocarditis underscores causal vulnerabilities in myocardial resilience to inflammatory insults, with prognosis varying inversely with diagnostic delay and ventricular dysfunction extent.[1][8]
Definition and Classification
Clinical and Pathological Definitions
Myocarditis is clinically defined as an inflammatory disease of the myocardium, the muscular layer of the heart wall, which may impair cardiac function through mechanisms including myocyte damage and edema.[9] Diagnosis typically relies on a combination of clinical presentation—such as acute chest pain, dyspnea, palpitations, or heart failure symptoms—elevated cardiac biomarkers like troponin, electrocardiographic abnormalities (e.g., ST-segment changes or arrhythmias), and imaging findings from echocardiography or cardiac magnetic resonance (CMR) demonstrating myocardial edema, hyperemia, or late gadolinium enhancement indicative of inflammation or fibrosis.[1][10] Non-invasive criteria, such as the Lake Louise criteria for CMR, support probable myocarditis when at least two of three features (T2-weighted edema, T1-weighted early gadolinium enhancement, and late gadolinium enhancement) are present, though these lack specificity and require correlation with clinical context.[11]Pathologically, myocarditis is confirmed via endomyocardial biopsy (EMB), the gold standard for definitive diagnosis, revealing inflammatory cellular infiltrates with associated myocyte necrosis or degeneration not characteristic of ischemic damage.[12] The Dallas criteria, established in 1984, classify active myocarditis as requiring both a mononuclear inflammatory infiltrate and myocyte necrosis, while borderline myocarditis features infiltrates without necrosis; these criteria emphasize light microscopy but have been critiqued for low sensitivity (detecting only about 10% of clinically suspected cases) due to sampling errors and focal inflammation.[13][14]Immunohistochemistry enhances detection by identifying immune cell markers (e.g., CD3+ T-lymphocytes, CD68+ macrophages) and viral genomes via PCR, addressing limitations of the Dallas criteria, though EMB remains underutilized due to procedural risks like perforation (0.5-1%).[15][10] The European Society of Cardiology (ESC) 2013 position statement integrates these for suspected myocarditis, recommending EMB in high-risk scenarios like fulminant presentation or unexplained heart failure.[11] Recent 2025 ESC guidelines maintain inflammation of the heart muscle as the core definition but emphasize etiology-specific patterns in histology.[16]
Etiologic and Histologic Classifications
Myocarditis is classified etiologically by underlying cause into infectious and non-infectious categories, with infectious etiologies accounting for the majority of cases worldwide. Viral infections predominate among infectious causes, including enteroviruses like coxsackievirus B, adenoviruses, parvovirus B19, and more recently SARS-CoV-2, often leading to direct myocyte invasion followed by immune-mediated damage. Bacterial causes such as Staphylococcus, Streptococcus, and Borrelia burgdorferi (in Lyme disease), fungal infections like Candida or Aspergillus in immunocompromised patients, and parasitic agents such as Trypanosoma cruzi (responsible for Chagas disease, affecting 6-7 million people globally with 30% progressing to cardiomyopathy) represent less common infectious triggers. Non-infectious etiologies encompass autoimmune processes linked to systemic conditions like sarcoidosis, systemic lupus erythematosus, or giant cell myocarditis; toxic exposures from drugs (e.g., immune checkpoint inhibitors, chemotherapy agents), alcohol, or chemicals; and hypersensitivity reactions. Up to 50% of adult cases and 82% of pediatric cases are deemed idiopathic after evaluation, though postviral immune dysregulation may underlie many such instances.[8][17][18]Histologic classification relies on endomyocardial biopsy examination, with the 1986 Dallas criteria defining myocarditis as an inflammatory infiltrate of the myocardium with necrosis or degeneration of adjacent myocytes, excluding ischemic damage; borderline myocarditis denotes sparse infiltrates without evident myocyte injury. These criteria, while foundational, exhibit limitations including sampling error (detecting histologic changes in only 25% of single biopsies), interobserver variability (confirmation rates as low as 64% among experts), and insensitivity to viral persistence without overt inflammation. Lymphocytic myocarditis, the most prevalent histologic subtype, is marked by diffuse T-cell and macrophage infiltrates with focal myocyte necrosis and is commonly associated with viral triggers. Eosinophilic myocarditis features eosinophil-predominant infiltrates, often without necrosis, and arises in hypersensitivity contexts such as drug reactions or hypereosinophilic syndrome. Giant cell myocarditis displays multinucleated giant cells, lymphocytes, eosinophils, and widespread necrosis, typically autoimmune in origin with aggressive progression absent immunosuppression. Other patterns include granulomatous forms (e.g., in sarcoidosis with epithelioid granulomas) and neutrophilic infiltrates in acute bacterial or early viral infections. [12][13][18]
Epidemiology
Incidence and Prevalence Rates
The incidence of myocarditis is estimated at 10 to 20 cases per 100,000 population annually in non-pandemic settings, based on clinically diagnosed cases in general populations. [19] Global Burden of Disease (GBD) analyses, which incorporate modeled data from vital registration, surveillance, and claims across 204 countries, report approximately 1.32 million incident cases worldwide in 2021, up from 791,000 in 1990, though age-standardized incidence rates have remained relatively stable or slightly declined due to demographic shifts. [20][21] These figures reflect primarily hospitalized or symptomatic cases, as subclinical myocarditis—detectable via biomarkers, imaging, or autopsy—is substantially underreported; autopsy series indicate pathological rates up to 10-fold higher in certain cohorts, such as young adults or post-viral events. [22]Prevalence data are sparse and less standardized, given myocarditis's predominantly acute course, with many cases resolving without chronic sequelae; GBD-derived estimates place global prevalence between 10.2 and 105.6 cases per 100,000, encompassing both active and resolved instances, but with wide uncertainty intervals reflecting diagnostic variability and modeling assumptions. [23][24] In high-income regions, national registries show lower rates: for instance, in Germany, age-adjusted incidence rose from 6.2 to 7.8 per 100,000 between 2007 and 2022, correlating with improved diagnostics like cardiac MRI rather than true epidemiological upticks. [22] In the United States, hospitalization rates increased from 9.5 to 14.4 per 100,000 over 2005–2014, driven by enhanced awareness and testing. [25]
These rates underscore diagnostic challenges: endomyocardial biopsy-confirmed cases remain rare (1–4 per 100,000), while non-invasive criteria inflate figures, potentially overestimating mild etiologies like viral triggers. [23] GBD models, while comprehensive, rely on imputations for low-data regions, introducing bias toward undercounting in developing areas where infectious causes predominate but surveillance lags. [25]
Demographic Risk Factors
Myocarditis demonstrates a pronounced male predominance in incidence and presentation across most age groups, with epidemiological studies reporting a male-to-female ratio ranging from 2:1 to 4:1.[26] This disparity is attributed to sex-specific immune responses, though the precise mechanisms remain under investigation; males under age 50 account for the majority of cases in viral and idiopathic forms.[27] In contrast, prevalence rates are higher in females among children younger than 5 years and adults over 95 years, potentially reflecting differences in early-life exposures or age-related vulnerabilities.[20]Age distribution shows a bimodal pattern, with elevated incidence in pediatric populations under 5 years—often linked to infectious etiologies—and a second peak in adolescents and young adults aged 15–44 years, comprising approximately 36.6% of U.S. myocarditis-related deaths from 1999–2022.[28] Young males in the 18–29 age group exhibit particularly high rates following viral illnesses like COVID-19, exceeding those in older cohorts.[29] Middle-aged individuals (45–74 years) represent the largest share of fatalities at 46.2%, while elderly cases (over 75) are less common but associated with comorbidities.[28]Racial and ethnic disparities in myocarditis outcomes are evident in U.S. data, though direct incidence risk factors by race remain less quantified. Hospital admissions are highest among non-Hispanic Whites (59.0%), followed by Blacks (19.5%) and Hispanics (12.8%), with Asians comprising a smaller proportion (2.8%).[30] Recent trends indicate sharper mortality increases among non-Hispanic American Indians/Alaska Natives (annual percent change of 14.9) and Hispanics (9.63), particularly in younger adults, potentially influenced by socioeconomic factors or access to care rather than inherent biological risks.[31] These patterns underscore the need for targeted surveillance in underrepresented groups, as global burden analyses highlight varying regional impacts but limited race-specific etiologydata.[23]
Temporal Trends and Post-Pandemic Shifts
Prior to the COVID-19 pandemic, the age-standardized incidence rate of myocarditis was estimated at 6.1 cases per 100,000 in males and 4.4 per 100,000 in females globally, based on 2019 Global Burden of Disease data.[32] Mortality rates associated with acute myocarditis in the United States exhibited a consistent decline from 1999 to 2019, reflecting improvements in diagnostic and therapeutic approaches, though incidence rates remained relatively stable with a slight upward trend in young males from 21.5 to 22.5 per defined population strata over prior decades.[31][33]The onset of the COVID-19 pandemic in 2020 marked an initial shift, with U.S. inpatient encounters for acute myocarditis increasing by 42% compared to pre-pandemic levels, coinciding with higher rates of viral infections including SARS-CoV-2, which carried a 16-fold elevated risk of myocarditis (0.146% incidence) versus non-COVID patients (0.009%).[31][34] Rates unrelated to COVID-19 infection or vaccination remained consistent at approximately 0.067-0.080% across 2020-2022 in large healthcare systems.[35]Mass rollout of mRNA COVID-19 vaccines from late 2020 onward correlated with a surge in myocarditis reports, particularly in males aged 12-29 years within 7 days post-second dose, with peak rates of 70.7 cases per million doses in adolescents aged 12-15.[36][37]Vaccine Adverse Event Reporting System (VAERS) data indicated myocarditis reports following COVID-19 vaccines in 2021 were 223 times higher than the average for all prior vaccines combined, exceeding expected background rates derived from 2017-2019 claims data.[38][39] This temporal clustering established a causal association, though absolute incidence remained rare (e.g., 0.48 per 100,000 overall per CDC estimates), with non-vaccine-related cases showing no similar escalation.[40]Post-2021, mortality rates for acute myocarditis rose notably from 2019 levels, reversing prior declines, amid ongoing vaccination campaigns and residual COVID-19 circulation.[33] While SARS-CoV-2 infection posed a higher relative risk (up to 17-fold in unvaccinated youth aged 12-30), the vaccine-associated subset introduced a distinct epidemiologic pattern: predominantly mild, self-resolving cases in young males, contrasting with more severe infection-linked presentations.[41][19] This shift highlighted mRNA vaccines as an iatrogenic factor in myocarditis epidemiology, though long-term trends post-2022 remain influenced by waning vaccination intensity and evolving viral strains.[42]
Etiology
Infectious Causes
Infectious agents represent a primary etiology of myocarditis, with viruses accounting for the majority of cases in developed countries, often following a prodromal respiratory or gastrointestinal illness.[43] Bacterial, protozoal, helminthic, and fungal infections contribute less frequently, typically in endemic regions, immunocompromised individuals, or through direct myocardial invasion or toxin-mediated damage.[3]Diagnosis of infectious myocarditis relies on clinical history, serology, PCR detection of pathogens in myocardial biopsies, and exclusion of non-infectious mimics, though persistent viral genomes are found in only about 38% of histologically confirmed cases.[44]
Viral Pathogens
Viral infections initiate myocarditis through direct cytopathic effects, immune-mediated myocyte damage, or persistent low-level replication leading to chronic inflammation.[43] Enteroviruses, particularly Coxsackievirus B3, are historically prominent, inducing acute necrosis and progressing to dilated cardiomyopathy in susceptible hosts.[43] Adenoviruses and parvovirus B19 are frequently detected in endomyocardial biopsies, with adenovirus identified in up to 23% of samples and parvovirus B19 implicated in adult cases, though its causal role remains debated due to bystander detection in controls.[44][43]Herpesviruses such as human herpesvirus 6 (predominantly HHV-6B, in 95% of associated cases), cytomegalovirus, and Epstein-Barr virus are linked to myocarditis, especially in immunocompromised patients or post-transplant settings.[43][3] Respiratory viruses including influenza A (cardiac involvement in 4.9% of hospitalized cases), SARS-CoV-2 (20-30% in severe COVID-19 with cardiac symptoms), and respiratory syncytial virus have surged in relevance, often presenting as fulminant disease.[43] In a multicenter U.S. cohort of 624 patients with biopsy-proven myocarditis, enteroviruses, adenoviruses, and cytomegalovirus were the most common viral genomes detected among the 38% positive samples.[44]
Non-Viral Infections
Bacterial myocarditis arises via hematogenous spread, toxin production, or superinfection, remaining rare outside specific contexts like sepsis or endocarditis.[43] Gram-positive pathogens predominate, including Staphylococcus aureus (common in disseminated infections with thrombus formation) and Streptococcus pneumoniae (inducing microlesions in up to 27-57% of meningococcal autopsies).[43]Borrelia burgdorferi causes Lyme carditis through spirochetal invasion, manifesting as atrioventricular block in endemic areas, while Corynebacterium diphtheriae toxins inhibit protein synthesis, leading to conduction abnormalities.[43][18]Parasitic infections are geographically restricted but significant; Trypanosoma cruzi underlies Chagas disease, progressing to cardiomyopathy in 20-30% of chronic cases via autoimmunity and fibrosis.[43]Toxoplasma gondii triggers necrotizing myocarditis in AIDS patients, and Plasmodium species contribute cardiovascular complications in 7% of severe malaria.[43] Helminths like Trichinella spiralis provoke eosinophilic infiltration.[43]Fungal myocarditis is opportunistic and often postmortem-diagnosed, affecting immunocompromised hosts via dissemination.[43]Candida species invade in 10-60% of fungemic cases, Aspergillus in transplant recipients, and Cryptococcus neoformans in 5-10% of AIDS-related infections, typically causing abscesses or diffuse inflammation.[43]
Viral Pathogens
Viral infections represent the predominant etiology among infectious causes of myocarditis, with enteroviruses, adenoviruses, and parvovirus B19 most commonly implicated through detection in myocardial tissue via polymerase chain reaction (PCR) assays.[43][45] Enteroviruses, particularly Coxsackievirus B serotypes, account for 25-40% of acute myocarditis cases in infants and young adolescents, often leading to severe neonatal presentations with high mortality, as evidenced by a 2022-2023 outbreak in France involving 20 severe cases confirmed by viral genome detection in cardiac tissue.[46][47] Adenoviruses are frequently associated with fulminant myocarditis in children, comprising up to 23% of cases in endomyocardial biopsies, with histological evidence of acute inflammation and viral persistence correlating with left ventricular dysfunction and increased mortality.[44][48]Parvovirus B19 genomes are detected in a significant proportion of myocarditis and dilated cardiomyopathy patients, with studies showing prevalence rates up to 40% in endomyocardial biopsies, suggesting a role in both acute and chronic myocardial inflammation through endothelial damage and T-cell mediated responses, though causality remains debated due to potential bystander persistence in non-inflamed tissue.[49][50]Herpesviridae family members, including human herpesvirus 6 (HHV-6), Epstein-Barr virus (EBV), and cytomegalovirus (CMV), have been identified in biopsy-proven cases, particularly in immunocompromised individuals, with HHV-6 linked to pediatric myocarditis via direct myocardial invasion.[51][52]Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection carries a myocarditis risk of 10 to 105 cases per 100,000 infected individuals, exceeding post-vaccination rates by factors of up to 35, with mechanisms involving direct viral entry via ACE2 receptors and cytokine storm-induced damage, confirmed in autopsy series showing viral RNA in cardiomyocytes.[53][54][55]Influenza viruses and other respiratory pathogens like respiratory syncytial virus contribute sporadically, often through molecular mimicry triggering autoimmunity rather than persistent infection.[45]Diagnosis typically requires endomyocardial biopsy for viralgenome detection, as serology alone lacks specificity, and many cases resolve spontaneously while chronic persistence may progress to dilated cardiomyopathy.[56]
Non-Viral Infections
Bacterial pathogens can induce myocarditis through direct myocardial invasion, toxin production, or post-infectious immune responses, though they represent a minority of cases compared to viral etiologies.[57] Common culprits include Staphylococcus and Streptococcus species, which often complicate bacteremia or endocarditis, leading to suppurative inflammation.[58] Diphtheria, caused by toxin-producing Corynebacterium diphtheriae, results in myocarditis in 10-20% of symptomatic cases, with myocardial damage mediated by the diphtheria toxin inhibiting protein synthesis in cardiomyocytes; fatality rates from diphtheritic myocarditis reach 60-80%, often within 6-10 days of onset.[59][60] Lyme disease, due to Borrelia burgdorferi, manifests as carditis in approximately 1% of reported cases, typically as atrioventricular block with variable degrees of myocardial inflammation, occurring weeks after initial infection in endemic areas.[61][62]Protozoal parasites are significant in endemic regions, with Trypanosoma cruzi (Chagas disease) being the leading non-viral infectious cause of myocarditis worldwide. In Latin America, where 6 million people are infected, 20-40% progress to chronic Chagas cardiomyopathy, characterized by patchy inflammation, fibrosis, and conduction abnormalities; acute myocarditis occurs in 1-2% of symptomatic infections, often with high parasitemia.[63][64] Other protozoa, such as Toxoplasma gondii, primarily affect immunocompromised hosts via disseminated infection, causing focal necrotizing myocarditis detectable by serology or biopsy.[57]Helminthic infections occasionally trigger eosinophilic myocarditis through larval migration or hypersensitivity. Trichinella spiralis (trichinellosis) leads to myocardial involvement in up to 20% of severe cases, with eosinophilic infiltration and potential arrhythmias.[57]Fungal myocarditis is rare and typically opportunistic, confined to immunocompromised individuals with disseminated disease. Candida species and Aspergillus spp. predominate, causing invasive myonecrosis or abscesses via hematogenous spread; Candida albicans accounts for most cases, often in patients with central lines or neutropenia.[58][18] Histoplasma and other endemic fungi can involve the myocardium in disseminated forms, but direct fungal myocarditis without endocarditis is exceptional.[57] Diagnosis often requires myocardial biopsy, as blood cultures may be negative.[57]
Non-Infectious Causes
![Histopathology of giant-cell myocarditis.jpg][float-right]
Non-infectious causes of myocarditis include autoimmune disorders, hypersensitivity reactions, toxic exposures, and iatrogenic factors such as certain medications and radiation therapy.[1] These etiologies often lead to immune-mediated myocardial inflammation without an identifiable infectious trigger, distinguishing them from viral or bacterial origins.[51] Systemic autoimmune diseases and drug hypersensitivity represent significant contributors, with histopathological patterns like giant cell or eosinophilic infiltration commonly observed.[65]
Autoimmune and Idiopathic
Autoimmune myocarditis occurs when dysregulated immune responses target myocardial antigens, frequently as part of systemic conditions such as systemic lupus erythematosus (SLE), where subclinical myocarditis affects up to 20% of patients based on autopsy studies.[66] Sarcoidosis, characterized by non-caseating granulomas in the myocardium, manifests as myocarditis in approximately 25% of cardiac involvement cases, often leading to arrhythmias or heart block.[67] Giant cell myocarditis, a rare idiopathic variant, features multinucleated giant cells and T-lymphocyte infiltration, with a fulminant course and median survival of 5.5 months without intervention; it associates with thymoma or other autoimmune diseases in 20-30% of cases.[65] Eosinophilic myocarditis links to hypereosinophilic syndromes or autoimmune vasculitides like eosinophilic granulomatosis with polyangiitis, driven by eosinophil-mediated toxicity.[68] Idiopathic forms, comprising up to 50% of biopsy-proven cases in some registries, likely encompass undetected autoimmune mechanisms, with lymphocytic infiltrates predominant on histology.[56]
Toxins, Drugs, and Iatrogenic Factors
Toxic myocarditis arises from direct myocardial injury by substances like heavy metals (e.g., lead, copper, iron), carbon monoxide, or hydrocarbons, which induce oxidative stress and necrosis; for instance, chronic lead exposure correlates with subclinical myocardial damage in occupational cohorts.[69][58] Drug-induced cases, often hypersensitivity-mediated, include clozapine, which causes myocarditis in 0.015-1.6% of schizophrenia patients typically within the first 4 weeks of therapy via eosinophilic infiltration and cytokine release.[70][71]Anthracyclines like doxorubicin produce dose-dependent toxic cardiomyopathy with myocarditis features, with cumulative doses exceeding 250 mg/m² increasing risk to over 10%.[18]Immune checkpoint inhibitors (e.g., nivolumab, pembrolizumab) trigger immune-related myocarditis in 0.06-1.14% of oncology patients, characterized by fulminant T-cell infiltration and high fatality rates up to 40%.[4]Radiation therapy for thoracic malignancies induces iatrogenic myocarditis through endothelial damage and fibrosis, with incidence rising to 10-30% in long-term survivors receiving doses over 30 Gy.[58] Other hypersensitivity triggers encompass antibiotics, anticonvulsants, and mesalazine, often presenting with eosinophilia.[72]
Autoimmune and Idiopathic
Autoimmune myocarditis arises from immune-mediated damage to the myocardium, distinct from infectious triggers, where the adaptive immune system erroneously targets cardiac self-antigens. This process involves autoreactive T lymphocytes and autoantibodies directed against myocardial proteins, leading to inflammation and myocyte necrosis.[4][73] Such reactions may occur as an isolated cardiac event or as a manifestation of systemic autoimmune disorders, including systemic lupus erythematosus, sarcoidosis, systemic sclerosis, and inflammatory bowel disease.[66][74] In these contexts, the immune dysregulation extends beyond the heart, with myocarditis contributing to cardiac dysfunction amid broader organ involvement.[73]A prototypical example is giant cell myocarditis (GCM), a rare and aggressive form featuring multinucleated giant cells, lymphocytes, and eosinophils infiltrating the myocardium, often resulting in rapid hemodynamic compromise or arrhythmias. GCM is linked to T-cell driven autoimmunity, with approximately 20% of cases co-occurring with other autoimmune conditions such as Crohn's disease.[74][75] Histopathologic examination reveals diffuse myocyte damage without viral inclusions, supporting an autoimmune etiology over persistent infection.[76] While immunosuppression, including corticosteroids and agents like muromonab-CD3, can improve outcomes in GCM, the condition carries high mortality without intervention, estimated at over 70% within months if untreated.Idiopathic myocarditis denotes inflammatory myocardial disease without an identifiable cause after standard evaluation, encompassing cases potentially driven by subclinical autoimmune mechanisms or unresolved post-infectious autoimmunity.[1] This category often overlaps with autoimmune forms, as advanced diagnostics like endomyocardial biopsy may uncover lymphocytic infiltrates suggestive of immune dysregulation even when systemic markers are absent.[67] Prevalence data for purely idiopathic cases remain imprecise due to diagnostic challenges, but they constitute a minority of acute myocarditis presentations, which overall affect 1 to 10 individuals per 100,000 annually in population studies.[17] Differentiation from autoimmune subtypes relies on excluding associated systemic diseases and negative serologic tests for specific autoantibodies.[73]
Toxins, Drugs, and Iatrogenic Factors
Drug-induced myocarditis encompasses inflammation of the myocardium triggered by therapeutic or illicit substances, often through hypersensitivity reactions, direct toxicity, or immune-mediated mechanisms.[69]Antipsychotic medications, particularly clozapine, represent a leading cause, accounting for approximately 59% of reported cases in pharmacovigilance analyses of drug-associated myocarditis.[70] Chemotherapy agents such as anthracyclines (e.g., doxorubicin) induce cardiotoxicity via oxidative stress and DNA damage, with cumulative doses exceeding 250-300 mg/m² elevating myocarditis risk.[71]Immune checkpoint inhibitors, used in cancer immunotherapy, are implicated in 10% of cases, manifesting as fulminant myocarditis with high mortality due to T-cell mediated myocardial injury.[70] Other pharmaceuticals, including mesalazine (6% of reports) and antibiotics like penicillin or sulfonamides, provoke hypersensitivity myocarditis characterized by eosinophilic infiltration.[58][70]Illicit drugs and environmental toxins contribute via sympathomimetic effects, direct cellular toxicity, or adulterant-related hypersensitivity. Cocaine use induces myocarditis through vasoconstriction, catecholamine surge, and potential contaminants like heavy metals, often presenting with eosinophilic infiltrates mimicking acute coronary syndrome.[77][78]Chronic alcohol overuse leads to toxic cardiomyopathy with inflammatory components, exacerbated by nutritional deficiencies and oxidative damage from acetaldehyde.[71]Heavy metals such as lead, mercury, arsenic, and cadmium promote myocardial inflammation via apoptosis induction and oxidative stress, though direct causation in isolated myocarditis is less common than broader cardiotoxicity.[79][80]Iatrogenic factors extend beyond pharmaceuticals to procedural interventions. Radiation therapy to the chest, typically for breast cancer or lymphoma, causes acute myocarditis in rare cases (e.g., within weeks of 50 Gy dosing) through vascular endothelial damage and inflammatory cytokine release, though chronic fibrosis predominates long-term.[81] Certain vaccines, including smallpox and influenza formulations, appear in pharmacovigilance data as triggers for hypersensitivity myocarditis.[82] mRNA-based COVID-19 vaccines are associated with elevated myocarditis risk, peaking in adolescent and young adult males (12-24 years) within 7-14 days post-second dose, with incidence rates up to 1 in 10,000-20,000 doses; histopathological findings include lymphocytic infiltrates without widespread necrosis, and the U.S. FDA has mandated labeling updates acknowledging this.[83][5][84]
Pathophysiology
Mechanisms of Myocardial Inflammation
Myocardial inflammation in myocarditis involves a biphasic process dominated by immune activation, where initial triggers—such as viral infection or autoantigens—prompt innate immune recognition followed by adaptive responses that amplify damage. In viral cases, pathogens like coxsackievirus B3 enter cardiomyocytes, engaging pattern recognition receptors including Toll-like receptors (TLRs) that detect viral nucleic acids, thereby inducing type I interferon production and upregulation of pro-inflammatory cytokines such as IFN-γ and TNF-α.[1][85] This innate phase recruits natural killer cells and macrophages, which release perforins, granzymes, and reactive oxygen species to eliminate infected cells, but excessive activity contributes to myocyte necrosis and release of damage-associated molecular patterns (DAMPs) that sustain inflammation.[8][51]Adaptive immunity escalates the response through T-cell mediated cytotoxicity, particularly CD8+ cytotoxic T lymphocytes that target viral peptides presented on MHC class I molecules, leading to direct lysis of infected and adjacent healthy myocytes.[56] CD4+ T helper cells, via Th1 polarization, further drive macrophage activation and cytokine release (e.g., IL-2, IL-12), while dysregulated responses can induce molecular mimicry or epitope spreading, where cross-reactive antibodies or T cells attack cardiac proteins like myosin, transitioning acute inflammation to autoimmune persistence.[86][87] In non-viral etiologies, such as hypersensitivity reactions to drugs or toxins, eosinophil infiltration predominates, mediated by IgE-dependent degranulation and release of major basic protein, causing toxic injury independent of adaptive specificity.[17]Giant cell myocarditis exemplifies severe autoimmune mechanisms, characterized by multinucleated giant cells derived from fused macrophages and T cells, driven by unchecked Th17 responses and IL-17 production that promote fibrosis alongside inflammation.[88] Across etiologies, chemokine gradients (e.g., CXCL10, MCP-1) facilitate leukocyte transmigration across the endothelium into the myocardium, where persistent cytokine signaling— including IL-1β and IL-6—impairs contractility and promotes remodeling, potentially culminating in dilated cardiomyopathy if unresolved.[87][89] These processes underscore the dual role of immunity: viral clearance versus collateral tissue destruction, with genetic factors like HLA associations influencing susceptibility and severity.[90]
Cellular and Molecular Damage Processes
In myocarditis, myocardial damage arises from both direct cytopathic effects of pathogens and dysregulated immune responses targeting infected or bystander cardiomyocytes. Viral replication within myocytes disrupts cellular homeostasis, inducing endoplasmic reticulum stress and impairing protein quality control mechanisms, which leads to accumulation of misfolded proteins and activation of unfolded protein response pathways.[91] This direct viral insult promotes myocyte necrosis and apoptosis through caspase-dependent pathways triggered by viral proteins interfering with host anti-apoptotic factors.[92] Necroptosis, a programmed form of necrosis involving receptor-interacting protein kinase 3 (RIPK3) and mixed lineage kinase domain-like protein (MLKL), emerges as a dominant cell death modality in acute viral myocarditis, exacerbating tissue injury beyond apoptosis.[93]Immune-mediated damage predominates in many cases, where infiltrating T lymphocytes, particularly CD8+ cytotoxic T cells, recognize viral antigens presented on major histocompatibility complex class I molecules and induce myocyte lysis via perforin and granzyme release.[88] CD4+ T helper cells amplify this process by secreting pro-inflammatory cytokines such as interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α), which upregulate Fas ligand expression on myocytes, promoting Fas-mediated apoptosis.[94] Natural killer cells contribute similarly through perforin-dependent cytotoxicity and IFN-γ production, enhancing susceptibility to severe myocardial necrosis in experimental models.[95] Th1-skewed responses, driven by interleukin-12 (IL-12), sustain this cytokine storm, including IL-2, IL-1, and monocyte chemoattractant protein-1 (MCP-1), which recruit additional leukocytes and perpetuate inflammation.[87][96]At the molecular level, exposure of intracellular cardiac proteins due to initial myocyte injury triggers autoantibody production and molecular mimicry, where immune responses cross-react with self-antigens like myosin, fostering chronic damage.[51] Endothelial activation and microvascular thrombosis further impair oxygen delivery, compounding ischemic necrosis independent of immune infiltration.[17] These processes collectively diminish cardiomyocyte contractility and electrical stability, with histological evidence of inflammatory foci accompanied by myocyte degeneration or overt necrosis defining diagnostic criteria.[17] Persistent T-cell activity can transition acute injury to fibrosis via transforming growth factor-beta signaling, underscoring the interplay between cell death modalities and repair failure.[94]
Clinical Manifestations
Acute Symptoms and Signs
Acute myocarditis often presents with a spectrum of symptoms reflecting myocardial inflammation and potential dysfunction, ranging from subclinical or mild flu-like illness to severe manifestations mimicking acute coronary syndrome or fulminant heart failure.[1] Common prodromal features include fever, malaise, and fatigue, frequently following a viral upper respiratory or gastrointestinal infection by days to weeks.[3] In symptomatic cases, patients typically report sudden-onset chest pain (prevalence 27-89% when concurrent pericarditis is present), dyspnea or orthopnea, and palpitations due to arrhythmias.[97] Syncope or presyncope occurs in a subset, often linked to ventricular tachyarrhythmias or bradyarrhythmias, while lightheadedness and exercise intolerance reflect reduced cardiac output.[1][51]Physical signs in the acute phase emphasize hemodynamic instability and cardiac involvement. Tachycardia disproportionate to fever is frequent, alongside hypotension or signs of cardiogenic shock in severe cases (e.g., cold extremities, oliguria).[1] Heart failure indicators such as jugular venous distension, pulmonary rales, S3 gallop, and peripheral edema may emerge rapidly, particularly in fulminant presentations affecting biventricular function.[97] A pericardial friction rub signals concomitant pericarditis, while murmurs from mitral regurgitation can arise from papillary muscle dysfunction or ventricular dilation.[1] Laboratory correlates like elevated troponins or BNP often accompany these findings, though they are not signs per se.[98]
Chest pain: Often pleuritic or pressure-like, exacerbated by lying supine; distinguishes from ischemia by younger age and viralprodrome in many.[58][79]
Systemic signs: Fever (up to 29% with flu-like onset), myalgias, and diaphoresis.[100]
Empirical data from cohorts indicate that up to 50% of acute cases may be asymptomatic or minimally symptomatic, detected incidentally via imaging or ECG abnormalities like ST elevation or low-voltage QRS.[101] Severe acute forms, however, carry high short-term mortality (up to 20-30% without support) due to pump failure or arrhythmias, underscoring the need for prompt recognition beyond vague fatigue.[1] Variability ties to etiology, with viral triggers more likely yielding prodromal symptoms than toxin-induced cases.[3]
Subacute and Chronic Presentations
Subacute myocarditis manifests 1 to 4 weeks after initial myocardial insult, marked by heightened autoimmune-mediated damage from activated T and B cells alongside antibody production, sustaining inflammation beyond the acute viral clearance phase.[3] Clinical features include ongoing fatigue, exertional dyspnea, and palpitations, often with subtle progression to mild heart failure signs such as orthopnea or peripheral edema, differing from acute fulminant cases by reduced incidence of severe chest pain or cardiogenic shock.[51] Arrhythmias, including ventricular ectopy or supraventricular tachycardia, may emerge due to persistent myocardial irritability, while diagnostic subtlety—such as milder troponin elevations and less pronounced ECG changes—can delay recognition.[102]Chronic myocarditis persists beyond 3 months, frequently evolving into inflammatory cardiomyopathy with fibrosis and myocyte loss, leading to ventricular dilation and systolic dysfunction in up to 30% of unresolved cases.[103] Symptoms center on progressive heart failure, encompassing chronic fatigue, reduced exercise capacity, nocturnal dyspnea, and lower extremity edema, alongside recurrent arrhythmias or conduction blocks that heighten sudden death risk.[104] In subsets like giant cell or lymphocytic variants, restrictive physiology may predominate, yielding diastolic impairment with preserved ejection fraction initially, though long-term prognosis includes 20-50% progression to transplant candidacy or mortality within 5 years, contingent on etiology and early intervention.[105][17]
Diagnosis
Initial Clinical Assessment
The initial clinical assessment for suspected myocarditis prioritizes a thorough history to establish clinical suspicion and guide subsequent evaluation. Patients are queried regarding recent viral prodromes, such as upper respiratory tract infections or gastroenteritis, which often precede viral myocarditis by 1-4 weeks.[1] Additional historical elements include potential exposures to toxins, medications (e.g., chemotherapy agents or immune checkpoint inhibitors), hypersensitivity reactions, or systemic autoimmune diseases like lupus erythematosus.[97] Symptom onset is typically acute, with reports of chest pain (present in 27-89% of cases, often pleuritic if pericarditis coexists), dyspnea on exertion, profound fatigue, palpitations, or syncope signaling arrhythmias.[97] Heart failure symptoms, such as orthopnea or peripheral edema, occur in 19-80% of acute presentations, while fever or myalgias may indicate ongoing inflammation.[97] In pediatric or adolescent cases, nonspecific complaints like abdominal pain or vomiting can predominate.[10]Physical examination focuses on hemodynamic stability and cardiac function, though findings vary widely from normal in subclinical disease to fulminant shock. Vital signs commonly show sinus tachycardia disproportionate to fever or volume status, with hypotension or orthostatic changes in hemodynamically significant cases.[1] Cardiovascular auscultation may reveal an S3 or S4 gallop, indicating ventricular dysfunction, or holosystolic murmurs from mitral or tricuspid regurgitation due to transient dilation.[97] Signs of right heart failure, including jugular venous distension, hepatomegaly, and lower extremity edema, suggest biventricular involvement, while pulmonary rales denote left-sided congestion.[58] Irregular rhythms or cannon A waves may point to conduction abnormalities.[51] In milder forms, the exam remains unremarkable, underscoring reliance on history for early suspicion.[106]Assessment stratifies risk: fulminant myocarditis presents with rapid cardiogenic shock and requires immediate intensive care, whereas subacute forms mimic idiopathic cardiomyopathy.[10] A high index of suspicion is essential in demographics like young males or athletes with unexplained ventricular ectopy post-viral illness, as delayed recognition elevates mortality risk.[51] This clinical foundation informs triage, with unstable patients prioritized for advanced monitoring before confirmatory testing.[106]
Diagnostic Tests and Biomarkers
Elevated cardiac troponin levels, particularly high-sensitivity troponin I or T, serve as key biomarkers indicating myocardial injury in suspected myocarditis, with studies showing elevations in up to 94% of biopsy-confirmed cases, though not specific to the condition as they can occur in other cardiomyopathies or ischemia.[107][108]Creatine kinase-MB (CK-MB) may also rise, reflecting muscle damage, but troponins are preferred due to higher sensitivity.[106] B-type natriuretic peptide (BNP) or N-terminal pro-BNP (NT-proBNP) levels correlate with ventricular strain and heart failure severity, aiding risk stratification but lacking diagnostic specificity for inflammation.[109][108]Inflammatory markers such as C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) are frequently elevated in acute myocarditis, reflecting systemic inflammation, but their non-specific nature limits utility, as normal levels do not exclude the diagnosis and elevations occur in infections or other inflammatory states.[106][110]Complete blood count may reveal leukocytosis or eosinophilia in specific etiologies like hypersensitivity myocarditis.[109] Viral serologies or PCR for pathogens (e.g., enteroviruses, parvovirus B19) support etiology but are not routine for initial diagnosis unless clinically indicated.[10]Electrocardiography (ECG) is a first-line test, detecting abnormalities in approximately 47-100% of cases, including ST-segment elevation mimicking infarction, T-wave inversions, PR depression, or arrhythmias like ventricular tachycardia, which guide urgency but have low specificity.[111][112] Transthoracic echocardiography evaluates global or regional wall motion abnormalities, ejection fraction reduction (often <50% in fulminant forms), or pericardial effusion, providing rapid bedside assessment of hemodynamic compromise without radiation risk.[10][113]Cardiac magnetic resonance imaging (CMR) with T2-weighted sequences for edema and late gadolinium enhancement (LGE) for necrosis/fibrosis represents the non-invasive reference standard, fulfilling Lake Louise criteria in 70-90% of cases with high sensitivity for subepicardial or mid-myocardial involvement atypical of ischemic patterns.[114][16] Guidelines from the European Society of Cardiology (ESC, 2025) and American College of Cardiology (ACC, 2024) recommend CMR in all hemodynamically stable patients with suspected myocarditis and elevated biomarkers or ECG/echo changes, as it informs prognosis (e.g., LGE extent predicts adverse remodeling) while avoiding biopsy risks like sampling error.[112][110] Emerging biomarkers like microRNAs (e.g., miR-21, miR-208b) show promise for specificity but remain investigational, not incorporated into standard protocols due to validation gaps.[108] No single biomarker or test confirms myocarditis definitively without clinical correlation, emphasizing multimodal approaches for probable diagnosis.[10]
Invasive Confirmation Methods
Endomyocardial biopsy (EMB) serves as the definitive invasive method for confirming myocarditis by providing histopathological evidence of myocardial inflammation and damage.[115] Performed under fluoroscopic guidance, the procedure typically involves catheter-based sampling from the right ventricular septum via femoral or jugular venous access, yielding 4-6 tissue fragments for analysis.[116] Histological evaluation identifies inflammatory infiltrates (e.g., lymphocytes, eosinophils, or giant cells) alongside myocyte necrosis or degeneration, enabling classification into active, borderline, or healed myocarditis.[12]Immunohistochemistry and molecular techniques, such as PCR for viral genomes, further enhance specificity by detecting immune activation markers (e.g., CD3+ T-cells) or pathogens.[15]The Dallas criteria, established in 1987, define myocarditis on biopsy as an inflammatory infiltrate with associated myocyte damage, excluding explanations like ischemia; borderline cases show infiltrates without necrosis.[13] These criteria exhibit approximately 60% sensitivity and 80% specificity, limited by focal disease distribution leading to sampling errors in up to 30-50% of cases.[1] EMB is recommended in scenarios unresponsive to standard therapy, such as new-onset heart failure with arrhythmias, suspected fulminant or giant-cell myocarditis, or to guide immunosuppression in histologically confirmed inflammatory cardiomyopathy.[117][112] Per 2024 ACC guidelines, EMB confirms etiology-specific subtypes (e.g., eosinophilic or sarcoid-related) and informs prognosis, with giant-cell variants showing <20% one-year survival without intervention.[112][118]Complications occur in 1-6% of procedures, including ventricular perforation (0.5-2%), pericardial effusion or tamponade requiring drainage (up to 2%), and rare mortality (0.1-0.5%), with risks elevated in hemodynamically unstable patients or those with elevated BNP levels.[119][120] Right ventricular EMB predominates due to lower perforation risk compared to left ventricular approaches (meta-analysisodds ratio 2.1 for effusion), though operator experience in high-volume centers (<1% major events) mitigates hazards.[121][122] Despite these risks, EMB's diagnostic yield justifies its use in ambiguous cases where non-invasive imaging (e.g., CMR) suggests inflammation but etiology remains unclear, as it uniquely identifies treatable causes like viral persistence or autoimmune triggers.[118] Left ventricular sampling may be pursued in right-sided failures or for broader sampling, but remains less common.[121]
Management
Supportive and Symptomatic Care
Supportive and symptomatic care for myocarditis emphasizes hemodynamic stabilization, symptom relief, and complication prevention, tailored to disease severity and stage, with hospitalization recommended for moderate to severe presentations involving heart failure, arrhythmias, or hemodynamic instability.[112][123] Continuous cardiac monitoring, supplemental oxygen for hypoxemia, and cautious fluid management are initial priorities in acute settings to address respiratory distress or shock, while avoiding excessive intravenous fluids in hypotensive patients to prevent worsening pulmonary edema.[123]Bed rest and restricted physical activity, particularly avoidance of strenuous exercise for 3-6 months in symptomatic or advanced stages, support myocardial recovery by reducing oxygen demand.[112]For heart failure symptoms, guideline-directed medical therapy (GDMT) is applied per established heart failure protocols, including diuretics for volume overload, angiotensin-converting enzyme inhibitors or alternatives for afterload reduction, and inotropes such as dobutamine or milrinone for low-output states in severe cases.[9][112][123] Beta-blockers may be introduced cautiously post-acute phase for arrhythmia prophylaxis or systolic dysfunction, though initiation is deferred in unstable patients due to negative inotropic effects.[9] Chest pain, often pericarditic, is managed with nonsteroidal anti-inflammatory drugs or colchicine when left ventricular ejection fraction is preserved, but these are avoided in reduced ejection fraction or fulminant cases to prevent hemodynamic compromise.[112]Arrhythmia management follows standard guidelines, with antiarrhythmic agents used judiciously for sustained ventricular tachycardia or significant bradyarrhythmias, and temporary pacing for advanced atrioventricular block; implantable devices like pacemakers or defibrillators are considered based on risk factors such as late gadolinium enhancement on imaging.[112][123][9] Sympathomimetics, beta-blockers in acute instability, and digoxin are generally avoided due to heightened myocardial sensitivity and proarrhythmic potential.[123] Ongoing monitoring includes serial electrocardiography, echocardiography, biomarkers like high-sensitivity troponin, and follow-up imaging at 2-4 weeks and 6 months to assess resolution and guide therapy adjustments.[112][9]
Targeted Etiologic Therapies
Targeted etiologic therapies for myocarditis focus on eradicating or mitigating the underlying causative agent or process when identifiable through diagnostic evaluation, such as endomyocardial biopsy or serologic testing, rather than relying solely on supportive measures.[9] In cases of infectious etiology, antimicrobial agents are employed to target the pathogen directly; for instance, antibiotics are the primary treatment for bacterial myocarditis, selected based on the specific organism, with examples including penicillin or erythromycin for streptococcal or borrelial infections like Lyme disease-associated myocarditis.[124][125] Similarly, for diphtheritic myocarditis, diphtheria antitoxin combined with antibiotics such as erythromycin is administered to neutralize toxin and eliminate Corynebacterium diphtheriae.[124] However, bacterial myocarditis remains rare, and empirical broad-spectrum coverage (e.g., vancomycin plus a third-generation cephalosporin) may be initiated pending culture results in suspected cases.[126]For viral myocarditis, which constitutes the majority of infectious cases, routine antiviral therapy is not recommended in acute presentations due to insufficient evidence of benefit from randomized trials, though agents like acyclovir, ganciclovir, or valacyclovir may be used for herpesvirus-associated disease, such as human herpesvirus 6 (HHV-6).[127][128] Interferon-beta has shown promise in small series for chronic enteroviral or parvoviral B19 myocarditis by reducing viral load, but larger studies are lacking, and its use is reserved for biopsy-confirmed persistent infection without contraindications.[125]Pleconaril, an enterovirus inhibitor, has been trialed in pediatric coxsackievirus cases with anecdotal recovery, yet prospective data remain limited.[125]In autoimmune or immune-mediated myocarditis, including giant cell myocarditis (GCM), immunosuppression is a cornerstone when viral persistence is excluded via biopsy. For GCM, a rapidly progressive variant with historically near-uniform fatality prior to 1990, combination therapy with high-dose corticosteroids (e.g., prednisone 60 mg/day), cyclosporine, and azathioprine yields 1-year survival rates exceeding 70% in prospective cohorts, compared to under 20% without intervention.[129][130]Muromonab-CD3 or ATG induction may be added for refractory cases, delaying or obviating transplantation.[74] For lymphocytic myocarditis without giant cells but with ongoing inflammation, the TIMIC trial demonstrated improved left ventricular function at 6 months with prednisone plus azathioprine versus placebo, but only in virus-negative biopsies, underscoring the risk of worsening outcomes with immunosuppression in active viralinfection.[128]Immune checkpoint inhibitor-induced myocarditis, increasingly recognized since 2016, responds to high-dose steroids (methylprednisolone 1-2 mg/kg/day), with infliximab or mycophenolate for steroid-refractory cases, achieving resolution in over 80% of reported series.[1]Hypersensitivity or drug-induced myocarditis requires immediate discontinuation of the offending agent, such as mesalazine or immune checkpoint inhibitors, often supplemented by brief corticosteroids if eosinophilic infiltration predominates.[9] Intravenous immunoglobulin (IVIG) at 2 g/kg over 24 hours has been used in pediatric viral or idiopathic cases for its antiviral and immunomodulatory effects, with meta-analyses showing reduced mortality in fulminant presentations, though adult evidence is weaker and not guideline-endorsed routinely.[131] Emerging targets like IL-1 blockade (anakinra) show efficacy in recurrent or pericarditis-overlapping cases but require further validation for primary myocarditis.[132] Overall, etiologic confirmation via biopsy is pivotal, as misapplication of immunosuppression in infectious contexts can exacerbate myocardial damage.[9]
Advanced Circulatory Support
Advanced circulatory support is indicated in myocarditis patients with fulminant presentation, characterized by rapid hemodynamic deterioration, cardiogenic shock, or cardiac arrest refractory to inotropic agents and fluid resuscitation.[133]Mechanical circulatory support (MCS) devices serve as a bridge to myocardial recovery, heart transplantation, or durable ventricular assist device implantation, providing time for diagnostic clarification and potential native heart function restoration.[134] In such cases, peripheral venoarterial extracorporeal membrane oxygenation (VA-ECMO) is frequently deployed as first-line temporary support due to its ability to rapidly achieve full biventricular and pulmonary support.[135]VA-ECMO involves cannulation of femoral vessels to divert blood from the right atrium to the arterial system post-oxygenation, though it increases left ventricular afterload, potentially necessitating adjunctive left ventricular unloading strategies like Impella or intra-aortic balloon pump (IABP) to prevent distension and pulmonary edema.[136] Survival to hospital discharge with VA-ECMO in fulminant myocarditis ranges from 55.7% to 75.5% in adults, with pediatric cohorts showing rates around 67-73%, often without requiring transplantation in survivors.[137][138] Factors favoring recovery include younger age, absence of pre-ECMO cardiac arrest, and preserved inflammatory markers with limited myocardial necrosis on admission.[139][140]Percutaneous left ventricular assist devices, such as Impella, offer targeted unloading and have been used in combination with ECMO (ECPella) to mitigate afterload issues, particularly in eosinophilic or giant cell myocarditis variants where recovery potential varies.[141] Durable MCS, including left ventricular assist devices (LVADs), is reserved for cases failing temporary support, with explantation possible upon recovery; however, myocarditis patients on durable MCS exhibit higher complication rates and lower bridging success compared to other etiologies.[142] MCS utilization in pediatric myocarditis has risen, occurring in approximately 1 in 13 cases, predominantly with ECMO, reflecting improved access to extracorporeal technologies.[143]Complications of MCS in myocarditis include bleeding (due to anticoagulation), vascular injury, stroke, and infection, with neurological events noted in up to 10-20% of ECMO cases; early aggressive support correlates with better outcomes, emphasizing rapid escalation before irreversible multiorgan failure.[138][135] In specialized centers, over two-thirds of patients with fulminant myocarditis and shock achieve discharge with MCS, underscoring its role despite inherent risks.[144]
Prognosis
Short-Term Outcomes and Mortality
In acute myocarditis, short-term outcomes are generally favorable for non-fulminant cases, with in-hospital mortality rates typically ranging from 2.4% to 5.3% across large cohorts. [22][145] For instance, a German nationwide analysis of over 88,000 hospitalizations from 2007 to 2022 reported an overall in-hospital mortality of 2.44%, with rates declining over time until a slight uptick post-2020. [22] Similarly, U.S. data from 1999 to 2019 indicated declining age-adjusted mortality for acute myocarditis, though a sharp increase occurred from 2020 onward, potentially linked to broader healthcare disruptions or diagnostic shifts rather than inherent disease progression. [31] Most patients experience resolution of symptoms within days to weeks with supportive care, though complications such as acute heart failure (occurring in up to 20-30% of hospitalized cases) or ventricular arrhythmias can prolong intensive care stays and elevate risks. [146]Fulminant myocarditis, characterized by rapid hemodynamic collapse requiring mechanical circulatory support, carries substantially worse short-term prognosis, with in-hospital mortality rates of 31-41%. [147][148] A meta-analysis of rapidly progressive cases reported a pooled short-term mortality of 35% (95% CI 29-40%), with lower rates in younger patients and those receiving timely extracorporeal membrane oxygenation (ECMO). [148] Survival to discharge in ECMO-supported fulminant cases has been documented at 59% in select cohorts, underscoring the role of early intervention, though overall 30-day mortality can reach 28% even with advanced therapies. [149][100] Biopsy-confirmed viral myocarditis shows 30-day all-cause mortality around 6-10% in clinically suspected acute presentations, higher in older adults or those with persistent left ventricular dysfunction. [150]Predictors of adverse short-term outcomes include advanced age, biventricular failure, and need for inotropes or ventilatory support, with odds ratios for 30-day mortality up to 3.75 times higher in acute myocarditis versus unexplained cardiomyopathy mimics. [151] Pediatric cases with mechanical support exhibit in-hospital mortality of approximately 22%, particularly in infants under 2 years. [152] Empirical data emphasize that while overall short-term survival exceeds 90% for milder forms, fulminant subsets demand aggressive monitoring to mitigate arrhythmia-related deaths, which account for a notable fraction of early fatalities. [153]
Long-Term Sequelae and Recovery Patterns
The prognosis of acute myocarditis varies by subtype and initial severity, with uncomplicated viral cases often resolving without residual impairment, while fulminant or biopsy-proven forms carry elevated risks of chronic sequelae such as dilated cardiomyopathy, heart failure, ventricular arrhythmias, and sudden cardiac death.[72] In biopsy-proven viral myocarditis, long-term all-cause mortality reaches 39%, with cardiac mortality at 27% and sudden cardiac death at 11%, reflecting progression driven by fibrosis and remodeling in approximately 30% of cases.[153][72]Fulminant myocarditis, characterized by acute hemodynamic collapse, demonstrates paradoxically favorable long-term outcomes among survivors, with 11-year transplant-free survival of 93% compared to 45% in acute nonfulminant myocarditis; left ventricular ejection fraction typically recovers fully in fulminant cases supported by mechanical devices, whereas nonfulminant forms more frequently evolve into persistent dysfunction or transplantation need.[154] Multicenter cohorts confirm this dichotomy: uncomplicated acute myocarditis yields 5-year major adverse cardiac event rates of 0%, including negligible heart failure or arrhythmias, versus 14.7% (encompassing death, transplantation, device implantation, or recurrent events) in complicated presentations with initial left ventricular ejection fraction below 50%.[101]Recovery patterns hinge on early intervention and etiology; among patients presenting with moderate-to-severe left ventricular dysfunction, 70% achieve preserved systolic function (ejection fraction ≥50%) at median follow-up of nearly 5 years, though overall mortality stands at 4.4%, rising to 27% in fulminant subsets due to in-hospital decompensation.[155] Adverse predictors include advanced age (hazard ratio 1.11 per year), poor functional class, and fulminant onset, while higher admission albumin levels correlate with reduced risk.[155] Even in clinically suspected cases without biopsy confirmation, 10-year all-cause mortality approximates 17%, underscoring persistent cardiovascular vulnerability beyond acute resolution.[156]
COVID-19 and Vaccine-Associated Myocarditis
Myocarditis Linked to SARS-CoV-2 Infection
Myocarditis has been reported in patients with SARS-CoV-2 infection, with estimated prevalence ranging from 10 to 105 cases per 100,000 infected individuals, showing a predominance in males.[53] Large cohort studies indicate that the one-year risk of myocarditis among COVID-19 survivors is approximately twofold higher compared to non-vaccinated individuals without prior infection.[157] In the United States, the overall incidence of myocarditis attributable to SARS-CoV-2 infection has been quantified through surveillance data, though exact rates vary by population and diagnostic criteria.[158]Proposed mechanisms include direct viral invasion of cardiomyocytes via ACE2 receptor expression, leading to cytopathic effects, as demonstrated in engineered human heart tissue models where SARS-CoV-2 replicated and induced cellular damage without infecting non-myocyte cardiac cells.[159] However, autopsy studies reveal infrequent detection of SARS-CoV-2genome in myocardial tissue, suggesting indirect pathways predominate, such as hyperinflammation, cytokine storm, and immune dysregulation triggering lymphocytic infiltration or endothelial dysfunction.[160] Additional contributors may involve hypoxia from severe respiratory disease, microvascular thrombosis, or dysregulation of the renin-angiotensin-aldosterone system, exacerbating myocardial injury.[161]Pathological confirmation remains challenging; autopsy series of fatal COVID-19 cases report myocarditis in 1.4% to 7.2% of hearts examined, often lacking classic viral inclusions and instead showing diffuse lymphocytic or macrophage infiltrates atypical of typical viral myocarditis.[162]Fulminant presentations, involving rapid hemodynamic collapse, have been documented in case series totaling over 100 patients, with high mortality despite mechanical support, though these represent severe outliers amid mostly subclinical or mild cases.[163] Comparative analyses estimate a 15-fold higher incidence of infection-associated myocarditis relative to non-SARS-CoV-2 etiologies, underscoring elevated risk during acute infection phases.[32]Risk factors align with severe COVID-19, including older age, comorbidities, and male sex, though younger patients can develop acute forms; empirical data from population studies confirm sustained post-infection cardiac risks persisting beyond acute illness.[164] Diagnostic reliance on elevated troponins, ECG changes, and echocardiography often precedes biopsy confirmation, which is rarely performed due to procedural risks in unstable patients.[165] These findings highlight SARS-CoV-2 as a trigger for myocardial inflammation, distinct from vaccine-related cases in temporal onset and potential severity.[54]
Myocarditis Following mRNA Vaccination
Cases of myocarditis have been observed following administration of mRNA-based COVID-19 vaccines, particularly Pfizer-BioNTech's BNT162b2 and Moderna's mRNA-1273, with reports emerging in early 2021.[5] The condition is characterized by inflammation of the heart muscle, confirmed via elevated cardiac biomarkers such as troponin, electrocardiographic changes, and cardiac magnetic resonance imaging showing late gadolinium enhancement indicative of myocardial injury.[166] Temporal clustering of cases within 2 to 7 days post-vaccination, especially after the second dose, supports a causal association, as evidenced by pharmacovigilance data and cohort studies excluding alternative etiologies in many instances.[36][167]Incidence rates are elevated compared to background population levels, with the highest risks in adolescent and young adult males aged 12 to 29 years.[168] In the United States, surveillance data from the Vaccine Adverse Event Reporting System (VAERS) identified 989 probable cases among individuals aged 12 to 29 between January 12 and November 5, 2021, predominantly after mRNA vaccination.[169] A study of over 2.5 million Israeli vaccine recipients found an incidence of myocarditis at 2.7 excess cases per 100,000 after the second BNT162b2 dose, rising to 10.69 cases per 100,000 in males aged 16 to 29.[167] Among U.S. military personnel, 23 cases were documented between January and June 2021, with an incidence of 8.2 per 100,000 in male service members under 30 after mRNA doses.[170] Moderna vaccination showed higher relative risk than Pfizer in some analyses, with odds ratios up to 2.5 for myocarditis in young males.[36]Symptoms typically include acute chest pain, dyspnea, and palpitations, prompting emergency evaluation.[171]Histopathology in biopsied cases reveals lymphocytic infiltrates with myocyte damage, distinct from classic viral patterns but consistent with immune-mediated injury potentially triggered by molecular mimicry or exaggerated T-cell responses to the spike protein encoded by the vaccine mRNA.[172] While most cases are mild and resolve with rest and anti-inflammatory therapy, a subset shows persistent late gadolinium enhancement on follow-up imaging, suggesting subclinical fibrosis in up to 80% of affected youth.[166] Hospitalization rates vary, but intensive care admission occurs in approximately 10-20% of reported cases, with rare progression to fulminant disease.[173]Mechanistic hypotheses include direct cardiotoxicity from the spike protein or adjuvant-induced hypersensitivity, supported by animal models and in vitro studies demonstrating cardiac cell uptake of lipid nanoparticles.[5] Risk mitigation strategies implemented by regulatory bodies include updated labeling warnings for males aged 12-24 as of June 2025 and recommendations for extended dosing intervals, which reduced incidence by up to 75% in observational data.[83][174] Despite these associations, absolute risks remain low at under 1 per 10,000 doses in high-risk groups, though underreporting in passive systems like VAERS may underestimate true incidence.[175] Ongoing surveillance through active systems continues to monitor long-term sequelae, including arrhythmias and reduced ejection fraction in select patients.[176]
Risk Comparisons and Empirical Data
Empirical studies have quantified the incidence of myocarditis following SARS-CoV-2infection as varying by age, sex, and infection severity, with rates generally exceeding those observed post-vaccination. Among males aged 12-17 years, approximately 450 cases per million infections have been reported, while broader population estimates indicate around 200 cases per million infections overall. In unvaccinated individuals under 40 years, the excess risk attributable to infection reached 16 additional cases per million in some analyses. For context, one large cohort study of over 20 million individuals found myocarditis occurring in about 0.2 per 1,000 (200 per million) infected patients within one year. These rates reflect confirmed diagnoses, often requiring hospitalization, and are derived from administrative health data linking infection status to cardiac outcomes.In contrast, mRNA vaccine-associated myocarditis shows lower incidence rates, predominantly after the second dose and in adolescent and young adult males. Rates peaked at 70.7 cases per million doses of BNT162b2 (Pfizer-BioNTech) in males aged 12-15 years, and around 67 cases per million in similar male cohorts post-second dose across mRNA platforms. Overall population incidence post-vaccination hovered at 5 cases per 100,000 (50 per million) exposures, with excess risks of 27.8 per million in head-to-head comparisons of mRNA vaccines. Booster doses elevated risks further, with incidence ratios up to 11.76 per million for mRNA-1273 (Moderna) in the 1-28 days post-dose.Direct comparative analyses consistently indicate higher myocarditis risk from SARS-CoV-2infection than from vaccination, with multipliers ranging from 2- to 15-fold depending on the cohort and timeframe. One self-controlled case series reported infection elevating risk 15-fold versus 2-fold post-vaccination, translating to more cases per exposure from infection. Another study across nearly 3 million cases found only 0.001% of myocarditis events tied to vaccination within 28 days, versus substantially elevated risks post-infection. In young males under 40, infection posed a sevenfold higher likelihood than vaccination. These findings hold across multiple jurisdictions, including U.S. and internationalsurveillance data, though absolute risks remain rare for both (under 0.1% incidence).
Population
Myocarditis Incidence per Million (SARS-CoV-2 Infection)
Myocarditis Incidence per Million (mRNA Vaccination, Post-2nd Dose)
Risk Ratio (Infection vs. Vaccination)
Males 12-17 years
~450
~67
~7-fold higher post-infection
Males 12-15 years
146-450 (variable by study)
70.7 (BNT162b2)
2-15-fold higher post-infection
Overall/Adult
200
50
4-7-fold higher post-infection
Severity profiles differ markedly, with vaccine-associated cases typically milder and resolving faster than those from infection. Post-vaccination myocarditis in young males often presents with chest pain, elevated troponins, and MRI-confirmed inflammation but low rates of intensive care (1.07% hospitalization, 0.015% mortality in affected cohorts), with most patients recovering fully within days to weeks via supportive care. Infection-linked myocarditis, by contrast, correlates with higher complication rates, including persistent cardiac dysfunction, arrhythmias, and 18-month sequelae like heart failure, particularly in hospitalized patients. One cohort comparison found fewer cardiovascular events (e.g., transplants, ventricular assist devices) in vaccine-attributed versus infection-attributed cases among young adults. These outcomes underscore causal distinctions: direct viral myocardial invasion and cytokine storms in infection versus immune-mediated responses post-vaccination, though both warrant monitoring.[164][54][177]
Criticisms of Risk Minimization Narratives
Critics contend that passive surveillance systems such as the Vaccine Adverse Event Reporting System (VAERS) substantially underestimate the incidence of mRNA vaccine-associated myocarditis, as voluntary reporting captures only a fraction of cases, with analyses estimating underreporting factors as high as 31 based on historical vaccine adverse event patterns and verification rates exceeding 90% for submitted myocarditis reports.[38][36] This underascertainment is compounded by diagnostic challenges, including reliance on symptomatic presentations and elevated biomarkers like troponin, potentially missing subclinical myocardial injury evidenced by widespread post-vaccination troponin elevations in military cohorts and athletes.[178]Comparative studies purporting lower myocarditis risk from vaccination versus SARS-CoV-2 infection have been faulted for methodological inconsistencies, such as drawing infection cohorts primarily from hospitalized or severe cases, which inflate observed rates by conflating disease severity with causation, while vaccination targets broader, healthier populations including low-risk youth where absolute infection-related myocarditis remains rare.[179]Retrospective designs in these analyses may further underestimate vaccine risks by missing transient or undiagnosed events, akin to historical undercounts in other vaccine-associated myocarditis like smallpox vaccination.[178] Moreover, risk-benefit narratives often emphasize relative risks without accounting for demographic mismatches, such as elevated odds (up to 11.76 per 100,000) in adolescent males post-second mRNA-1273 dose, a group facing minimal severe COVID-19 threat pre-Omicron.[179]Long-term sequelae challenge assurances of uniformly mild outcomes, with cardiac magnetic resonance imaging revealing persistent late gadolinium enhancement—a marker of myocardial fibrosis and potential precursor to arrhythmias, dilated cardiomyopathy, or sudden death—in 78% or more of followed vaccine-myocarditis patients at 5-18 months post-onset.00388-2/fulltext) 00282-7/fulltext) [180] Such findings, alongside reports of relapsing inflammation and cardiac arrest temporally linked to vaccination, underscore uncertainties in minimization claims, particularly from sources like public health agencies with mandates to promote uptake, where incentives may prioritize infection-comparison framing over absolute risks or extended monitoring in affected cohorts.[181][182] These critiques highlight the need for prospective, unbiased longitudinal data to assess causal impacts beyond short-term hospitalization metrics.
Historical Developments
Early Case Reports and Recognition
The earliest documented descriptions of myocardial inflammation appeared in the mid-18th century, when French physician Jean-Baptiste Senac detailed cases of heart muscle inflammation in his 1749 treatiseTraité de la structure du cœur, de son action, et de ses maladies, highlighting the diagnostic challenges posed by its subtlety during life.[183] Senac's observations, drawn from clinical and postmortem examinations, marked an initial step toward distinguishing inflammatory processes in the myocardium from other cardiac pathologies, though without microscopic confirmation.[184]The term "myocarditis," denoting inflammation specifically of the heart muscle, was formally introduced in 1837 by German pathologist Joseph Friedrich Sobernheim, who used it to describe non-ischemic inflammatory changes observed in autopsied tissues.[57] This nomenclature helped differentiate myocarditis from endocarditis or pericarditis, but early reports remained anecdotal, often conflated with rheumatic fever or idiopathic heart failure, limiting clinical recognition until advances in histology.Histological case reports emerged in the late 19th and early 20th centuries, with Russian pathologist Abramov documenting an autopsy of primary myocarditis in 1897.[185] Recognition advanced significantly in 1900 when pathologist Carl Ludwig Alfred Fiedler reported a series of fatal cases in young adults, characterizing acute interstitial myocarditis through microscopic evidence of diffuse lymphocytic infiltrates in the myocardial interstitium, unassociated with coronary artery disease or valvular issues, and leading to fulminant heart failure with death occurring 5–17 days post-onset.[185] Fiedler's findings, based on systematic autopsy reviews, established myocarditis as a distinct, often rapidly lethal entity, prompting subsequent pathological scrutiny; for instance, Christian Georg Schmorl's re-examination of Fiedler's slides identified subtypes including lymphocytic and giant-cell infiltrates, linking them to potential infectious or autoimmune triggers.[185] These reports shifted focus from gross anatomy to cellular pathology, enabling earlier antemortem suspicion in cases of unexplained acute cardiac decompensation.
Key Diagnostic and Therapeutic Milestones
The histopathological recognition of myocarditis emerged in the late 19th century following the adoption of microscopy for autopsy examinations, with Carl Ludwig Alfred Fiedler providing the first detailed description of acute interstitial myocarditis in 1900, characterized by inflammatory infiltrates and myocyte damage.[186][187] Prior to this, the term "myocarditis" had been introduced by Joseph Friedrich Sobernheim in 1837 to denote myocardial inflammation, though without standardized diagnostic criteria.[183] These early postmortem findings established inflammation as a causal mechanism in sudden cardiac deaths but limited clinical diagnosis to inference from symptoms like chest pain and arrhythmias.The advent of endomyocardial biopsy (EMB) in 1962, initially developed in Japan for invasive cardiac sampling, marked a pivotal shift toward antemortem diagnosis, enabling direct histological confirmation of myocarditis in living patients by the 1960s.[188][189] This technique, performed via right heart catheterization, allowed identification of inflammatory cells and myocyte necrosis, transforming myocarditis from a largely presumptive entity to one verifiable through tissueanalysis. In 1984, the Dallas criteria standardized EMB interpretation, defining active myocarditis as lymphocytic infiltrates with myocyte damage and borderline cases as infiltrates without necrosis, facilitating consistent histopathological diagnosis despite sampling limitations.[12][190]Noninvasive imaging advanced diagnostics further with the original Lake Louise criteria in 2009, which integrated cardiac magnetic resonance (CMR) features—such as T2-weighted edema, early gadolinium enhancement for hyperemia, and late enhancement for necrosis/scarring—to support myocarditis diagnosis without biopsy in select cases.[191] Updated in 2018, these criteria incorporated parametric mapping (native T1, T2, and extracellular volume) for quantitative assessment of inflammation and fibrosis, improving sensitivity to approximately 80-90% in acute presentations while reducing reliance on invasive EMB, which has a sensitivity of 10-30% due to focal disease.[192] High-sensitivity cardiac troponin assays, refined in the 2000s, complemented these by detecting myocardial injury with levels elevated in over 90% of confirmed cases.[112]Therapeutically, management remained supportive through the mid-20th century, focusing on heart failure symptoms with diuretics, inotropes, and vasodilators, as no etiology-specific interventions existed beyond treating precipitating infections.[123] The 1980s introduced mechanical circulatory support like intra-aortic balloon pumps and ventricular assist devices for fulminant cases, reducing short-term mortality from near 100% to 20-50% in specialized centers by bridging to recovery or transplant.[193] EMB-guided immunosuppression emerged as a milestone in the 1990s for biopsy-proven lymphocytic or giant-cell myocarditis, with regimens including corticosteroids and cyclosporine or azathioprine yielding 5-year survival rates of 70-80% versus 20% without, though randomized trials remain limited due to rarity.[9] For viral etiologies, interferon-beta trials in the 2000s showed modest viral clearance but no consistent mortality benefit, underscoring supportive care as the mainstay.[8] Recent advances emphasize etiology-targeted approaches, such as intravenous immunoglobulin for pediatric cases or colchicine for pericarditis-dominant presentations, integrated with guideline-directed heart failure therapy including ACE inhibitors and beta-blockers.[111][9]