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Potato leafroll virus

Potato leafroll virus (PLRV) is a single-stranded positive-sense RNA virus belonging to the species Potato leafroll virus in the genus Polerovirus and family Solemoviridae, known for causing potato leafroll disease in potato crops (Solanum tuberosum) worldwide.^[1] This virus features an icosahedral virion approximately 25-30 nm in diameter, with a genome of about 5.9 kb encoding multiple open reading frames that facilitate replication, movement, and aphid transmission.^[1] First described in 1916 in the Netherlands as the causative agent of leafroll symptoms in potatoes, PLRV remains one of the most economically significant potato pathogens due to its persistent spread via infected seed tubers and aphid vectors.^[2] Infected potato plants typically exhibit primary symptoms in upper leaves, including upward rolling, paling, and erect posture, often accompanied by chlorosis and reddening at the leaflet edges, while secondary infections lead to severe rolling and leathery texture in lower leaves, plant stunting, and reduced photosynthesis.^[3] Tuber quality is compromised, with internal net necrosis—characterized by brown strands or speckles—appearing in susceptible varieties, rendering produce unmarketable.^[4] PLRV is transmitted in a circulative, non-propagative manner by several aphid species, primarily the green peach aphid (Myzus persicae), requiring at least 20 minutes of feeding for acquisition, followed by a latent period of at least 24 hours in the vector before inoculation is possible, with the virus persisting in the vector for days to weeks.^[4]^[3] Although potatoes are the primary host, PLRV infects other solanaceous crops such as tomato (Solanum lycopersicum) and pepper (Capsicum annuum), as well as weeds like shepherd's purse (Capsella bursa-pastoris) and jimsonweed (Datura stramonium), serving as reservoirs for aphid-mediated spread.^[4]^[3] The virus is distributed globally across all major potato-growing regions, including North and South America, Europe, Asia, Africa, and Oceania.^[4] Economically, PLRV causes yield losses of up to 90% in severely affected fields, primarily through reduced tuber size and number, alongside quality degradation that disqualifies seed lots from certification standards (typically tolerating 0.5-5% infection).^[4] Management relies on planting certified virus-free seed, aphid control with insecticides, and rogueing infected plants, though challenges persist due to the virus's efficient vector transmission and genetic diversity.^[3]^[5]

Virology

Taxonomy and classification

The Potato leafroll virus (PLRV) is classified as a member of the genus Polerovirus within the family Solemoviridae and the order Sobelivirales, according to the International Committee on Taxonomy of Viruses (ICTV).^[1]^[6] PLRV was first described in 1916 by Dutch researchers H.M. Quanjer, H.A. van der Lek, and J.G. Oortwijn Botjes, who identified the infectious nature of potato leafroll disease through grafting experiments in the Netherlands.^[7] Initially termed "potato leafroll disease," the causal agent was later confirmed as a virus, with purification of particles achieved in 1967, leading to its formal naming as PLRV and placement in the luteovirus group before reclassification into the genus Polerovirus in 1999.^[7]^[8] Its taxonomic placement is defined by key characteristics, including a phloem-limited lifestyle and a positive-sense single-stranded RNA (+ssRNA) genome approximately 5.9 kb in length, which encodes multiple open reading frames essential for replication and aphid transmission.^[9]^[10] PLRV exhibits strain variations, with classical isolates such as PLRV-0 and PLRV-5 showing nucleotide identities of 92-98% and differing in aphid transmissibility and symptom severity; an emerging variant identified in Scotland in 2023-2024, characterized by reduced genetic diversity in the 3' genome region following aphid transmission, has rapidly become predominant, leading to increased infection rates in seed potatoes by 2025.^[10]^[11]

Genome and virion structure

The Potato leafroll virus (PLRV) has a monopartite, linear, positive-sense single-stranded RNA genome of approximately 5.9 kb, specifically 5,881–5,889 nucleotides in length across isolates.^[12]^[13] This genome features seven overlapping open reading frames (ORFs 0–6), expressed via genomic RNA for the 5'-proximal ORFs and subgenomic RNAs for the others, encoding proteins critical for viral replication, suppression of host defenses, movement, and assembly.^[14] ORF0 produces the P0 protein, a multifunctional silencing suppressor that also aids in symptom induction and contains an F-box motif for targeting host proteins; ORFs 1 and 2 encode the P1 and P2 replicase subunits, where P1 includes protease and helicase domains for genome-linked protein processing, and P2 serves as the RNA-dependent RNA polymerase core.^[7]^[13] ORFs 3–5 encode the P3 movement protein for cell-to-cell and systemic trafficking, the P4 coat protein essential for virion formation, and the P5 readthrough extension of P4 that enhances capsid stability and aphid transmission efficiency.^[14] The PLRV virion is a non-enveloped, isometric particle with icosahedral symmetry, approximately 25 nm in diameter, composed of 180 copies of the major coat protein P4 arranged in T=3 morphology, along with a few copies (typically 3–6) of the minor readthrough protein (P4-P5 fusion) that project from the surface to facilitate vector interactions.^[7]^[15] Assembly initiates with P4 encapsidating the genomic RNA, where the readthrough domain of P5 stabilizes the particle and exposes motifs for aphid binding, ensuring efficient transmission without altering the core icosahedral structure. PLRV exhibits low genetic diversity globally, with nucleotide identities among isolates typically exceeding 95%, serving as a threshold for strain delineation within the species; for instance, Indian isolates share 97.6–98.7% similarity with reference strains, while Argentine and worldwide sequences range from 94.4–97.3%, reflecting evolutionary constraints in replicase and coat regions.^[13]^[16]^[14]

Transmission

Vectors and acquisition

The primary vector of Potato leafroll virus (PLRV) is the green peach aphid, Myzus persicae, which efficiently transmits the virus in a persistent circulative manner.^[17] Secondary vectors include the glasshouse potato aphid, Aulacorthum solani, and Macrosiphum euphorbiae, though these species transmit PLRV less effectively than the primary vector.^[18]^[19] Aphids acquire PLRV by ingesting phloem sap from infected plants during an acquisition access period, after which the virus crosses the gut epithelium into the hemolymph without replicating in the vector.^[17] The virus then circulates in the hemolymph for a latent period of 24-48 hours before reaching the accessory salivary glands, where it binds to the GroEL chaperonin protein produced by the aphid's endosymbiotic bacterium Buchnera aphidicola to prevent degradation and facilitate retention.^[20]^[21] Transmission efficiency increases with longer acquisition access periods, often exceeding 80% after 16 hours of feeding on infected tissue.^[22] Colonizing aphid species like M. persicae are preferred vectors for PLRV due to their tendency to establish prolonged feeding on potato phloem, unlike transient non-colonizing aphids that contribute minimally to spread.^[23] PLRV acquisition can alter vector behavior, including enhanced settling and phloem ingestion on infected plants as well as changes in reproduction rates, potentially increasing the virus's epidemiological success.^[24] Non-vector transmission of PLRV is limited to experimental grafting between infected and healthy plants or rare mechanical inoculation under artificial conditions, but these routes are negligible in field settings compared to aphid-mediated dissemination.^[7]

Inoculation and persistence

The inoculation of Potato leafroll virus (PLRV) into host plants occurs when viruliferous aphids, primarily Myzus persicae, feed on phloem tissue and inject virus particles through their salivary glands during the probing and ingestion phases.^[25] This process requires a minimum inoculation access period (IAP) of approximately 1 hour for efficient transmission, though longer periods (e.g., 24-48 hours) are often observed in field conditions to achieve higher infection rates.^[26] Once the virus reaches the plant's phloem, it establishes systemic infection, facilitated by the aphid's targeted feeding behavior that delivers virions directly to vascular tissues.^[27] PLRV persists in the aphid vector for its entire lifespan, typically up to 30 days under optimal conditions, in a circulative, non-propagative manner where virions accumulate in the hemocoel and salivary glands without replicating in the insect.^[28]^[29] The virus is transmitted transstadially, passing through aphid molts from nymph to adult stages, but not transovarially to progeny, ensuring retention solely in acquired individuals.^[30] Transmission efficiency in competent vectors reaches 50-80% under laboratory conditions, influenced by factors such as aphid age and viral titer.^[31]^[32] Vector specificity for PLRV transmission is determined by interactions between viral coat and readthrough proteins with specific receptors on the aphid midgut epithelium, allowing efficient uptake and circulation only in certain species.^[33] At least 11 aphid species can transmit PLRV, but M. persicae and Macrosiphum euphorbiae are the most efficient, with transmission rates varying by species-specific binding affinities.^[34] Winged (alate) morphs of these aphids play a key role in long-distance dispersal, carrying the virus across fields and regions during migratory flights.^[35] Quantitative models of PLRV transmission in potato fields incorporate aphid population dynamics, vector competence, and crop density to estimate the basic reproduction number (R₀), which typically exceeds 1 under high aphid pressure, indicating potential for epidemic spread.^[36] These models highlight that R₀ is sensitive to vector control measures, with reductions below 1 achievable through targeted interventions.^[36]

Hosts and symptoms

Primary and alternative hosts

The primary host of Potato leafroll virus (PLRV) is potato (Solanum tuberosum), where the virus establishes systemic infections primarily through contaminated seed tubers or aphid vectors. In potatoes, seed tuber transmission occurs at rates up to 100% from tubers produced by secondarily infected plants, perpetuating the virus as a major source of primary inoculum in new plantings.^[26] Alternative hosts encompass over 20 plant species, mainly within the Solanaceae family, including tomato (Solanum lycopersicum), tobacco (Nicotiana tabacum), and weeds such as black nightshade (Solanum nigrum), hairy nightshade (Solanum sarrachoides), and groundcherry (Physalis floridana). These alternative hosts, particularly solanaceous weeds, serve as reservoirs that support aphid-mediated spread to potato crops. Experimental or occasional natural infections have been documented in non-Solanaceae species like shepherd's purse (Capsella bursa-pastoris) in Brassicaceae and spinach (Spinacia oleracea) in Chenopodiaceae, but they do not constitute significant reservoirs.^[37]^[2]^[4] PLRV infections are confined to phloem tissues across all hosts, restricting mechanical transmission and limiting the overall host range to phloem-dependent species. No natural reservoirs outside the Solanaceae family have been identified as epidemiologically important for sustaining PLRV cycles in potato production systems. Infected seed tubers generally yield a high percentage of infected daughter plants (typically 70-90%, though variable from 36-100% depending on cultivar and infection stage), reinforcing the virus's persistence through vegetative propagation.^[38]

Symptoms and diagnosis

Infection with Potato leafroll virus (PLRV) in potato plants manifests through distinct primary and secondary symptoms, depending on whether the infection occurs during the growing season or is carried over from infected seed tubers. Primary symptoms, resulting from aphid transmission during the season, include pale, upright, and rolled upper leaves with reddening around the edges, while lower leaves may show minimal changes.^[3] Secondary symptoms, from tuber-borne infection, feature severe upward rolling and leathery texture in lower leaves, overall plant stunting, chlorosis, and an upright growth habit, with marginal reddening or chlorosis in older leaves.^[3] Additionally, infected tubers often develop internal net necrosis, particularly in susceptible varieties like Russet Burbank, appearing as brown, corky arcs in the vascular ring.^[28] In alternative hosts, PLRV symptoms vary in severity; tomatoes exhibit milder effects such as leaf mottling, puckering, and upward curling without significant stunting.^[39] In weeds like jimsonweed (Datura stramonium), infections can cause more severe systemic necrosis and chlorosis, contributing to plant decline.^[3] Diagnosis of PLRV relies on a combination of serological, molecular, and biological methods to confirm infection, as foliar symptoms can be subtle or absent. Enzyme-linked immunosorbent assay (ELISA), particularly double antibody sandwich ELISA (DAS-ELISA), detects PLRV coat protein in leaf or tuber extracts with a sensitivity allowing detection at dilutions up to 1:250 of infected tissue.^[40] Reverse transcription polymerase chain reaction (RT-PCR) targets the open reading frame 5 (ORF5) region of the PLRV genome for highly specific amplification, enabling detection in low-titer samples from leaves or tubers at RNA dilutions of 1:1024 or greater.^[41] Visual indexing involves grafting or mechanical inoculation onto indicator plants like Physalis floridana, where symptoms such as leaf rolling and chlorosis appear within 3-4 weeks to confirm infectivity.^[42] Asymptomatic or latent infections complicate field detection, occurring in up to 17.5% of samples that test positive via molecular methods despite lacking visible symptoms.^[43] Such cases underscore the need for routine serological or PCR-based screening in seed certification programs to identify hidden reservoirs.^[44]

Epidemiology

Disease cycle

The disease cycle of Potato leafroll virus (PLRV) commences with overwintering primarily in infected potato seed tubers, which serve as the main reservoir between seasons, and to a lesser extent in perennial weeds such as field bindweed (Convolvulus arvensis).^[45] Infected volunteer potato plants and certain wild hosts like wild crucifers can also harbor the virus over winter.^[2] During spring planting, emergence of plants from these infected tubers introduces the virus into new fields, establishing focal points of infection that attract colonizing aphids.^[29] Primary infection arises from seed-borne PLRV, where the proportion of infected tubers in the seed lot directly determines initial field incidence, often reaching high levels such as up to 52% in uncertified seed lots.^[46] This seed-mediated introduction provides a persistent source within the crop, contrasting with secondary infection driven by aphid vectors such as the green peach aphid (Myzus persicae), which facilitates rapid dissemination.^[45] Secondary spread accelerates exponentially during summer as aphid populations expand, enabling the virus to infect healthy plants nearby and across fields.^[47] Upon aphid inoculation into phloem tissue, PLRV exhibits a latency period of 2-3 weeks before symptoms manifest in current-season infections, allowing time for systemic spread.^[48] The virus moves exclusively through phloem sieve tubes, limited to this vascular compartment, which restricts its distribution but enables efficient long-distance transport within the plant.^[49] PLRV epidemics exhibit population dynamics characterized by buildup models incorporating aphid vector proliferation, often following logistic growth patterns that align with seasonal increases in aphid density and corresponding rises in virus incidence.^[47] These models highlight how initial primary foci amplify through secondary transmission, culminating in widespread infection by harvest.^[50]

Environmental factors

Transmission of Potato leafroll virus (PLRV) is highly influenced by temperature, with optimal conditions for aphid-mediated spread occurring between 13 and 19°C, where vector acquisition and inoculation efficiency peak due to favorable aphid physiology and virus retention.^[51] At temperatures exceeding 26°C, transmission rates decline sharply owing to elevated mortality and shortened lifespan of the primary vector, Myzus persicae.^[52] Below 5°C, PLRV stability diminishes, leading to reduced infectivity in infected plant tissues during storage or overwintering.^[53] Relative humidity plays a critical role in vector dynamics, as high levels (80–90%) promote aphid population growth and enhance PLRV transmission efficiency by up to 35% when paired with moderate temperatures around 25°C.^[54] In contrast, low humidity and dry conditions restrict aphid alate production and flight capability, thereby limiting long-distance virus dispersal.^[55] Soil properties and light exposure exert indirect effects on PLRV through modulation of host plant vigor; nutrient-deficient soils weaken potato plants, amplifying disease severity and yield impacts in infected crops.^[56] Shaded environments delay symptom onset, such as leaf rolling, by slowing photosynthesis and phloem-limited virus movement within the host.^[29] Climate change, including warmer temperatures, may influence overwintering of aphid vectors and volunteer plants, potentially affecting PLRV reservoirs.

Global distribution and prevalence

Potato leafroll virus (PLRV) is ubiquitous in potato-growing regions worldwide, occurring across Europe, North America, South America, Asia, Africa, and Oceania, where it ranks as the second most prevalent potato virus after potato virus Y (PVY).^[5] Its distribution aligns closely with commercial potato production, facilitated by the movement of infected seed tubers and aphid vectors.^[57] Prevalence varies by region, with higher incidence often reported in temperate zones supporting dense aphid populations. In Europe, particularly the United Kingdom and Scotland, PLRV incidence in seed potato crops has reached 9.4% of the seed area surveyed in 2022, escalating to affect 17.5% of Scottish seed crops in 2025 through downgrades or failures.^[58]^[59] In North America, historical epidemics linked to environmental cycles have demonstrated widespread occurrence, while in South America, the virus contributes to significant infections in native potato microcenters.^[60]^[61] Asia shows notable rates, such as 20-60% incidence in potato plains of Punjab and Sindh, Pakistan, and up to 52.3% in surveyed fields.^[62] In Africa, PLRV is present but with variable incidence; for instance, in Kenya, 72.9% of field samples tested positive for at least one major potato virus (with PLRV at 6.8%), and surveys in Kenya and Nigeria confirm its distribution, though lower aphid pressure in some arid areas limits explosive outbreaks compared to temperate regions.^[63]^[14]^[64] Prevalence trends indicate rising incidence in certain areas, notably a 15-20% increase in Scotland following the emergence of novel PLRV variants since 2018, which accumulate to higher levels and displace older strains.^[65]^[66] Globally, estimates suggest 20-50% of seed lots may be infected, underscoring the virus's persistence in propagation material despite certification efforts.^[2] Key factors driving PLRV distribution include international trade in certified seed potatoes, which mitigates but does not eliminate spread when latent infections occur, and aphid migration corridors, such as wind-assisted dispersal of vectors like Myzus persicae, enabling rapid jumps between fields and regions.^[2]^[67] Monitoring data from organizations like the Agriculture and Horticulture Development Board (AHDB) and Science and Advice for Scottish Agriculture (SASA) reveal annual increases of 5-10% in PLRV incidence within temperate zones, based on visual inspections and serological testing of seed crops.^[58]^[67] These surveys emphasize the need for ongoing vigilance, as genetic diversity—documented in 84 full-genome sequences from 22 countries—highlights evolving populations that challenge control measures.^[5]

Impact

Economic losses

Potato leafroll virus (PLRV) significantly impacts potato production by causing substantial yield reductions in infected plants, with tuber weight losses ranging from 30% to 90% depending on the potato cultivar, infection timing, and environmental conditions.^[2] In severe cases, primary infections can lead to near-total crop failure in susceptible varieties, while secondary infections from contaminated seed tubers typically result in 33-50% reductions.^[68] Globally, PLRV is estimated to cause an annual loss of approximately 20 million tons of potatoes, underscoring its role as one of the most economically damaging potato pathogens.^[69] In the United States, PLRV inflicts annual economic losses of around $100 million, primarily through diminished yields and quality issues in commercial potato fields.^[70] These costs are exacerbated in developing countries, where limited access to certified virus-free seed tubers amplifies infection rates and results in higher losses relative to production scales.^[71] PLRV also degrades tuber quality through internal net necrosis, a symptom characterized by brownish, net-like discolorations in the vascular tissue that render tubers unsuitable for fresh markets or processing.^[72] In processing industries, 20-30% of tubers from moderately infected fields may be discarded, further compounding financial strain on growers.^[73] Beyond direct yield and quality losses, PLRV elevates production costs for farmers through the need for intensive monitoring, aphid vector control, and seed certification programs. These indirect expenses highlight the virus's broader toll on potato industry sustainability, particularly in regions reliant on export markets.^[74]

Physiological effects on plants

Potato leafroll virus (PLRV) infection primarily disrupts phloem function in potato plants by inducing excessive callose deposition in sieve tube elements, which obstructs the transport of assimilates and photosynthates. This phloem-limited replication of the virus leads to necrosis in phloem tissues of stems and petioles, as well as abnormal accumulation of callose in sieve tubes of stems and tubers, impairing the translocation of carbohydrates from source leaves to sink organs such as tubers.^[75]^[22] Consequently, carbohydrates accumulate in infected leaves, reducing photosynthate allocation to developing tubers and contributing to diminished tuber yield and quality.^[26] PLRV also induces hormone imbalances that affect plant growth and development. Infection modulates phytohormone signaling, including suppression of jasmonic acid (JA) and ethylene (ET) pathways through virus-encoded proteins such as P0, P1, and P7, which inhibit aphid-induced hormone responses and weaken plant defenses.^[76] These imbalances disrupt overall growth regulation and exacerbate the physiological stress on infected plants. PLRV interacts with the host genome by suppressing RNA silencing mechanisms, facilitating viral replication and systemic spread. The virus-encoded P0 protein acts as a potent suppressor of RNA interference (RNAi) by binding to and promoting the degradation of ARGONAUTE1 (AGO1), a key component of the host's RNAi machinery, thereby preventing the degradation of viral RNA.^[77] This suppression allows the virus to evade host defenses and establish persistent infection in phloem tissues.^[33] Recent studies (as of 2025) indicate that PLRV infection alters source-sink dynamics and triggers antioxidant responses to mitigate oxidative stress from reactive oxygen species (ROS), with reductions in tuber starch content in susceptible varieties.^[78]

Management

Cultural and agronomic practices

Cultural and agronomic practices play a crucial role in preventing the introduction and spread of Potato leafroll virus (PLRV) in potato fields by minimizing inoculum sources and vector activity. These methods emphasize the use of high-quality planting material and field management to interrupt the disease cycle, particularly through aphid transmission.^[79] Seed certification programs are essential for ensuring virus-free stock, with certified seed tubers typically required to have PLRV infection levels below 0.5-1% for elite classes and up to 2% for standard certified seed, varying by region and standards, to limit primary infection from planting material. These programs involve rigorous inspections, including visual assessments during the growing season and post-harvest winter grow-out tests to detect latent infections. Roguing infected plants early, by removing symptomatic individuals and neighboring plants to account for asymptomatic spread, further reduces inoculum within the field and supports recertification standards.^[80]^[79]^[81] Crop rotation with non-host crops, such as cereals or grasses, for at least two to three years helps break the disease cycle by eliminating volunteer Solanaceae plants that serve as reservoirs for PLRV. Sanitation practices, including the destruction of cull piles to prevent sprouting and the prompt removal of infected or volunteer potatoes, are critical to eliminating overwintering sources of the virus. Avoiding Solanaceae weeds and groundkeepers in and around fields further limits potential hosts for aphids carrying PLRV.^[29]^[81]^[79] Strategic planting timings and configurations can reduce exposure to aphid vectors during peak flight periods. Early planting allows potatoes to reach maturity before aphid populations surge in mid-summer, thereby minimizing transmission opportunities. Incorporating barrier or trap crops, such as mustard or cereals like wheat and sorghum around field borders, acts as a filter to intercept and deter viruliferous aphids from reaching the potato crop.^[82]^[79]^[83] Regular field monitoring through scouting for virus symptoms, such as leaf rolling and stunting, and aphid presence maintains low inoculum levels by enabling timely roguing. Indexing techniques, including serological tests like ELISA on leaf samples, help quantify PLRV incidence and guide management decisions, particularly in seed production fields where thresholds are strictly enforced. These practices collectively support sustainable PLRV control without relying on chemical interventions.^[29]^[79]^[81]

Chemical and biological controls

Chemical control of Potato leafroll virus (PLRV) primarily targets its aphid vectors, such as the green peach aphid (Myzus persicae) and potato aphid (Macrosiphum euphorbiae), through insecticide applications to limit virus transmission within fields.^[84] Systemic insecticides like imidacloprid, applied at rates of 0.1-0.2 kg active ingredient per hectare via in-furrow or soil drench at planting, provide early-season protection by uptake into plant tissues, reducing aphid colonization for up to 60 days.^[85] Foliar sprays, including pyrethroids or neonicotinoids, are timed to coincide with aphid influx periods, typically from early to mid-season, to suppress winged migrants and prevent secondary spread.^[29] To mitigate insecticide resistance in aphid populations, rotations among chemical classes (e.g., from neonicotinoids to organophosphates) are recommended, following integrated pest management principles.^[86] Biological controls leverage natural enemies to suppress aphid vectors without relying solely on synthetic chemicals. Predatory insects, such as lady beetles (Coccinellidae spp.), consume large numbers of aphids, with larvae alone capable of eating up to 300 individuals per development cycle, contributing to population reductions in potato fields.^[87] Parasitoid wasps of the genus Aphidius (e.g., Aphidius ervi and Aphidius matricariae) lay eggs inside aphids, leading to host mummification and up to 80% parasitism rates in targeted populations when released augmentatively.^[88] Entomopathogenic fungi, particularly Beauveria bassiana, infect aphids through cuticle penetration, achieving 50-70% mortality within 7-10 days under favorable humidity conditions (above 70% RH), as demonstrated in greenhouse and field trials on solanaceous crops.^[89] Treatment decisions are guided by economic injury levels, with thresholds of 5-10 aphids per plant (or 5 per leaf in processing potatoes) triggering interventions to balance control efficacy against costs, especially in seed production where even low vector densities can amplify PLRV incidence.^[88] Integrated pest management (IPM) for PLRV vectors combines chemical and biological approaches with regular monitoring using yellow sticky traps or plant scouting, enabling targeted applications that reduce overall insecticide use by 30-50% while maintaining yield protection.^[79] This strategy emphasizes early detection of aphid alates to time interventions, conserving beneficial insects and minimizing non-target effects.^[84]

Resistance breeding and genetic strategies

Natural resistance to Potato leafroll virus (PLRV) in potato has been identified primarily from wild relatives such as Solanum etuberosum, which confers extreme resistance through the dominant Rl_retb gene, preventing viral multiplication and systemic spread in infected plants.^[90]^[91] This resistance is stably inherited across sexual generations, making S. etuberosum a valuable source for introgression into cultivated potato (Solanum tuberosum).^[90] Another key source is S. tuberosum ssp. andigena, harboring the Rl_adg allele, which provides major gene resistance to PLRV accumulation by limiting viral replication in phloem tissues.^[92]^[93] Breeding programs have successfully incorporated the *Rl_adg* allele into elite cultivars, achieving substantial protection against PLRV, with resistant lines showing up to 90% reduction in virus titer compared to susceptible varieties.^[94] These efforts often involve interspecific hybridization followed by backcrossing to restore tuber quality and yield, though polygenic nature of full resistance complicates complete transfer.^[95] Marker-assisted selection (MAS) has accelerated progress by targeting quantitative trait loci (QTLs) for PLRV tolerance, including a major QTL (PLRV.1) on chromosome XI that explains over 50% of variation in virus accumulation resistance.^[96]^[97] For instance, the cultivar 'Superior' exhibits partial field tolerance to PLRV, linked to QTLs on chromosomes V and XI, enabling deployment in regions with moderate aphid pressure without full immunity.^[98] Genetic engineering approaches, particularly RNA interference (RNAi), have targeted PLRV open reading frames (ORFs) such as the coat protein (CP; ORF 3) and movement protein (MP; ORF 4) to induce post-transcriptional gene silencing.^[99] Transgenic potatoes expressing inverted-repeat RNAi constructs against PLRV CP reduced infection rates by 80-100% in greenhouse and field trials, eliminating systemic symptoms and tuber necrosis.^[98]^[100] Similarly, RNAi targeting the replicase gene (ORF 1) has conferred broad resistance by disrupting viral RNA replication, with efficacy demonstrated in multiple cultivars.^[101] CRISPR/Cas9 editing enhances PLRV resistance by modifying host susceptibility factors or bolstering endogenous silencing pathways, such as knocking out eIF4E isoforms to block viral translation initiation.^[102] Edited lines show reduced PLRV titer and delayed symptom onset, with up to 90% protection in edited progeny, though off-target effects remain a challenge.^[103] These strategies complement RNAi by enabling precise, non-transgenic modifications in elite germplasm. Recent advancements in the 2020s include regulatory approvals for GM potatoes expressing the Rl_adg gene, such as events deregulated in the US in 2024, providing stacked resistance to PLRV and other viruses while addressing biosafety concerns through field trials confirming no environmental risks. As of 2025, a novel PLRV variant identified in Scotland has led to increased infection rates in seed fields (up to 17.5%), necessitating enhanced monitoring and management, while projects like BioPotatoes are advancing precision-bred varieties incorporating natural PLRV resistance traits to reduce reliance on insecticides.^[104]^[94]^[11]^[105] Progress in overcoming regulatory hurdles has facilitated commercialization in select regions like North America, with ongoing efforts to integrate CRISPR edits into breeding pipelines for durable, multi-virus resistance.^[94] Challenges persist in polyploid genome complexity and aphid vector dynamics, but combined conventional and biotechnological strategies promise enhanced PLRV management.^[106]

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Aulacorthum solani (foxglove aphid) | CABI Compendium
A. solani is an important vector of Potato virus Y, Potato virus A, Potato virus X and Potato leafroll virus (Culjak et al., 2013).
[19]
Potato Leaf Roll Virus - PLRV - the French seed potato - Fn3Pt
Few aphid species can transmit PLRV: Myzus persicae is the most efficient vector but Aulacorthum solani and Macrosiphum euphorbiae may also transmit PLRV.
[20]
The Role of Bacterial Chaperones in the Circulative Transmission of ...
Generally, the latent period for luteoviruses can be as short as 24 hours; however it can also reach up to four days [14]. While early reports suggested that ...
[21]
Potato Leafroll Virus Binds to the Equatorial Domain of the Aphid ...
PLRV readily binds to extracellular Buchnera GroEL, and in vivo interference in this interaction coincides with reduced capsid integrity and loss of ...
[22]
[PDF] ACQUISITION AND TRANSMISSION OF POTATO LEAFROLL ...
Although PLRV is persistently transmitted by several aphids species, Myzus persicae Sulzer is considered to be the principal and most efficient vector ( ...<|control11|><|separator|>
[23]
[PDF] Aphid vectored virus disease in potato crops - Farm Advisory Service
Secondly and more importantly, several species of aphid can act as vectors for potato viruses. Aphid vectored viruses are a much greater concern than feeding ...
[24]
Changes in Green Peach Aphid Responses to Potato Leafroll Virus ...
Oct 1, 2009 · The green peach aphid, Myzus persicae (Sulzer), an important insect pest of potato, Solanum tuberosum L., is the most efficient vector of potato ...
[25]
Potato Diseases Caused by PVY and PLRV - Cooperative Extension ...
Potato Leaf Roll Virus (PLRV) is transmitted by aphids, primarily, the green peach aphid. When an aphid picks up the virus from an infected plant, the virus ...
[26]
Details of DPV Potato leafroll virus and References
Infectious nature of potato leafroll disease described by Quanjer, Van der Lek & Oortwijn Botjes (1916), virus particles purified by Peters (1967). Selected ...Missing: discovery history
[27]
First report on molecular basis of potato leaf roll virus (PLRV ...
Nov 10, 2020 · Aphids acquire the PLRV into the salivary glands by feeding on infected plants and further continue to transmit the virus to healthy plants ...
[28]
Potato (Solanum tuberosum)-Potato Leafroll Virus (Leaf Roll)
Once an aphid acquires the virus, it can transmit it for life, but not pass it on to its offspring. The green peach aphid is the most important vector in our ...Missing: circulative lifelong
[29]
Leafroll / Potato / Agriculture: Pest Management ... - UC IPM
Potato leafroll virus is not transmitted mechanically by machinery or contact with leaves. Management. Use certified seed tubers. Control sources of the virus, ...Missing: graft | Show results with:graft
[30]
[PDF] Evidence for Lack of Propagation of Potato Leaf Roll Virus in its ...
major aphid vector, Myzus persicae (Sulzer). Insect extracts or potatoes exhibiting typical PLRV symptoms and the PLRV hemolymph were used as inocula ...Missing: sulcicola | Show results with:sulcicola
[31]
[PDF] Transmission of Potato Leafroll Virus from Plants and Artificial Diets
* After the acquisition access period (AAP), the aphid nymphs were transferred five times to fresh Physalis floridana seedlings at inoculation access periods ( ...
[32]
Transmission efficiency of potato leaf roll virus (Plrv) by potato aphid ...
Aug 2, 2025 · Five aviruliferous aphids of both the species were allowed to acquire the PLRV from virus infected potato plant for 96 hrs (Acquisition Access ...
[33]
Molecular Mechanisms of Potato Plant–Virus–Vector Interactions
It helps the virus move long distances through the plant's vascular system. Although its mechanism of action is not fully understood, mutations in the P3a gene ...
[34]
Potato leafroll virus molecular interactions with plants and aphids
Potato leafroll virus (PLRV) genome organization with a 5.8–5.9 kb positive-sense single-stranded RNA genome, typical of poleroviruses. Open reading frames ( ...Potato Leafroll Virus... · 2. Transmission By Aphids... · 4. Virus Management And...
[35]
Aphid populations and virus vector potential in potato fields across ...
Oct 21, 2025 · Over 40 aphid species including the notorious Myzus persicae and Macrosiphum euphorbiae are known virus vectors in potato fields.
[36]
Addressing Various Strategies for Combating the Leaf-Roll Virus in ...
Mar 25, 2025 · In this research, we introduced a mathematical simulation that describes the transmission dynamics of the potato leaf-roll virus (PLRV) and ...
[37]
First Report of Twenty-two New Hosts of Potato leafroll virus
Twenty-two new hosts of Potato leafroll virus were identified, including Cucurbita pepo, Datura fastuosa, and Hibiscus golfrosens, in 5 new families and 1 ...
[38]
tomato disease - PlantVillage
Oct 24, 2016 · Potato leafroll virus (PLRV) Tomato spotted wilt virus (TSWV) ... This virus causes varied symptoms like mosaic, mottling or puckering or curling ...
[39]
[PDF] DAS-ELISA PLRV - Bioreba
May 16, 2024 · PLRV (Potato leafroll virus). Genus. Polerovirus. Method. DAS-ELISA ... PLRV on leaves of potato: 1:250 dilution. PLRV on tubers of potato ...
[40]
Sensitive detection of potato viruses, PVX, PLRV and PVS, by RT ...
Primers were designed from the coat protein gene of all three viruses and were able to detect virus in leaf RNA dilutions of 1 : 1024 to 1 : 4096 and from tuber ...
[41]
Indexing of Potato leaf roll virus (PLRV) from Potato Growing Areas ...
Tubers and leaves of symptomatic potato plants were indexed for PLRV using test plants, DAS-ELISA and RT-PCR.
[42]
Prevalence of potato viruses on potato (Solanum tuberosum L ...
Asymptomatic leaf samples showed positive reactions to viruses (PVX, PVY and PLRV) at a rate of 17.5%. Meanwhile, 38.7% of symptomatic samples tested negative ...
[43]
[PDF] Detection of Potato Leafroll Virus in Dormant Potato Tubers
A further advantage of IMC/RT-PCR on ELISA tests can be mentioned. A positive result with the latter method does not prove the presence of viable virus.
[44]
[PDF] POTATO LEAFROLL VIRUS DISEASE
An aphid may transmit PLRV for a long time after acquisition and the latent period has passed. Occasionally, an aphid transmits PLRV for its entire life- time, ...
[45]
Responses of Aphid Vectors of Potato leaf roll virus to Potato Varieties
Jul 28, 2017 · Potato leaf roll virus (PLRV) can reduce tuber yield and quality in potato. Green peach aphid (Myzus persicae [Sulzer]) and potato aphid ...Skip main navigation · Abstract · Materials and Methods · Results
[46]
Regression Modeling Strategies to Predict and Manage Potato Leaf ...
It was concluded that a five-year model validated with a two-year data set exhibited 94% variability in the PLRV disease development. All environmental ...
[47]
Potato Leaf Roll Virus - Crop IPM
Plants from infected tubers show symptoms first on lower leaves within a month after emergence. Current season infection develops after aphids probe on potato ...
[48]
Inhibited Long‐Distance Movement of Potato Leafroll Virus to ...
Jun 28, 2008 · PLRV is introduced by aphids directly into phloem tissue. The virus probably moves in plants mainly in sieve tubes, because of phloem limitation ...
[49]
Eco-epidemiological model and analysis of potato leaf roll virus ...
Therefore, this paper developed a fractional order mathematical model of PLRV by considering aphid vectors. The results indicate that decreasing the infection ...Missing: logistic growth
[50]
Temperature and CO2 Level Influence Potato leafroll virus Infection ...
Oct 1, 2017 · In this study, we found that an increase in atmospheric CO 2 concentration (940 ± 50 ppm) resulted in the infection of more potato plants by PLRV at 30 ± 2°C ...Missing: latency | Show results with:latency
[51]
Effects of High and Fluctuating Temperatures on Myzus persicae ...
Dec 1, 2006 · Optimal temperature for green peach aphid population growth was 26.7°C. The lower and upper developmental thresholds were 6.5 and 37.3°C, ...Abstract · Materials and Methods · Results
[52]
[PDF] Effect of Storage Temperatures on Potato Virus Infectivity Levels
Leaves infected with potato leafroll virus or potato virus A, S, X, or Y stored at 25, 4,-20, or -70 C produced variable infectivity and serological ...
[53]
Environmental factors influencing aphid transmission of potato virus ...
High RH (80–90%) and high temperature (25–30°C), when combined, increased virus transmission by 30–35%. Transmission rates were reduced by nearly 50% if RH was ...
[54]
Myzus persicae (green peach aphid) | CABI Compendium
Dec 18, 2021 · Aerial dispersal of winged forms over long distance is the main mode of dispersal of this cosmopolitan pest. However, phytosanitary measures are ...
[55]
First report on molecular basis of potato leaf roll virus (PLRV ... - NIH
The crop is infected by more than 30 RNA viruses, out of which 13 are mainly transmitted by aphids. Potato leaf roll virus (PLRV), belongs to genus Polerovirus ...<|control11|><|separator|>
[56]
Potato virus Y and Potato leafroll virus management under climate ...
Nov 26, 2020 · Abstract · Potato virus Y (PVY) and Potato leafroll virus (PLRV) are the two major potato viruses threatening profitable seed potato production.
[57]
Potato Leafroll Virus - an overview | ScienceDirect Topics
Potato Leafroll Virus is a plant virus that specifically affects potatoes, causing high yield losses. It is transmitted through infected potato seed tubers ...
[58]
Turning the tide on potato virus - Crop Production Magazine
Apr 17, 2023 · “Potato leaf roll virus (PLRV) seems to be increasing apace and it's a ... PLRV incidence was recorded at 9.4% of the 2022 seed area ...<|control11|><|separator|>
[59]
New variant of leafroll virus - British Potato
Oct 21, 2025 · PLRV incidence has risen sharply in Scottish seed potatoes and number of seed potato fields reporting infection has increased nearly tenfold.
[60]
Epidemics of potato leaf roll in North America and Europe linked to ...
Potato leafroll virus: Evaluation of resistance in potato cultivars. Plant Dis. 70: 621-623. Bahlme, H.N., and D.A. Mooley. 1981. Cyclic fluctuations in the ...
[61]
Virus Incidence Associated with Native Potato Yield in Microcenters ...
Oct 16, 2021 · The most frequently detected viruses are those that are contact-transmitted, with PVX (incidence 30–82%) and PVS (20–50%) the most common, ...
[62]
[PDF] A REVIEW PAPER ON POTATO LEAF ROLL VIRUS (PLRV) OF ...
coding for proteins (P0-P7) and Rap1 respectively. Three 5' proximal ORFs encode the proteins (P0, P1 and P2). Five other ORFs are expressed from two ...
[63]
Prevalence, distribution and control of six major potato viruses in ...
Dec 7, 2020 · Virus prevalence in the fields was high; 72.9% of the samples were positive for at least one of the six viruses; and 55.9% showed multiple ...
[64]
Incidence and Distribution of Potato Viruses in Plateau State, Nigeria
Abstract. Potato farms and backyard holdings in Plateau State were surveyed for potato viruses S,X,Y and leaf roll in 1990-1992.
[65]
Genetic Diversity of Potato Leafroll Virus (Polerovirus PLRV) Is ...
Potato leafroll virus (PLRV) is a positive-strand RNA virus which belongs to the species Polerovirus PLRV within the family Solemoviridae [1,2,3,4]. PLRV is a ...<|control11|><|separator|>
[66]
[PDF] Genetic diversity of potato leafroll virus is shaped by variant ...
Aug 24, 2025 · The PLRV genome contains eight open reading frames (ORFs), some of which overlap. The viral RNA replicase is . CC-BY-NC 4.0 International ...Missing: organization | Show results with:organization
[67]
Summary & Outlook | SASA (Science & Advice for Scottish Agriculture)
Inspection of Scottish seed potato crops over the past few years have revealed an increasing incidence of both PVY and PLRV - the two predominant aphid-borne ...Missing: prevalence | Show results with:prevalence
[68]
Potato Leafroll Virus - an overview | ScienceDirect Topics
Potato leafroll virus (PLRV) is a viral pathogen that affects potato plants and can lead to significant crop damage. Resistance to PLRV has been engineered ...
[69]
Scientists discover key plant virus-insect virus interaction | CALS
Jun 11, 2019 · Potato leafroll virus (PLRV) is of particular concern, as it can reduce ... 20 million tons of global yield losses annually. “The most ...
[70]
How genetic engineering is creating more nutritious, healthier food ...
May 18, 2022 · Potato leafroll polerovirus is responsible for the annual loss of 20 million tons of potato, at a cost of US$100 million. ... Virus, which ...<|separator|>
[71]
Addressing Various Strategies for Combating the Leaf‐Roll Virus in ...
Mar 21, 2025 · In this research, we introduced a mathematical simulation that describes the transmission dynamics of the potato leaf-roll virus (PLRV) and involves some ...
[72]
Landscape‐scale patterns and predictors of potato viruses in Scotland
Mar 15, 2024 · This approach provides a comprehensive overview of potato viruses in Scotland, a deeper understanding of epidemiological risk factors at the landscape-scale ...<|control11|><|separator|>
[73]
[PDF] PEST MANAGEMENT IN THE FUTURE - National IPM Database
On fresh market stock PLRV is rarely an economic problem. On processing potatoes PLRV can cause net necrosis (internal discoloration) of the tubers in some ...
[74]
[PDF] Pest Management Strategic Plan for Pacific Northwest Potato ...
Jan 26, 2006 · Develop pest management strategies that lower inputs/costs for growers while maintaining the sustainability of potato production. 3. Investigate ...
[75]
Potato Leafroll Virus - an overview | ScienceDirect Topics
Potato leafroll virus is a member of the genus Polerovirus in the family Solemoviridae. Potato leafroll virus (PLRV) has a worldwide distribution and is one ...Missing: taxonomy classification<|control11|><|separator|>
[76]
https://doi.org/10.1111/pce.13684
[77]
Virus‐induced phytohormone dynamics and their effects on plant ...
Feb 8, 2021 · Virus infection of plants may also modulate other phytohormones, such as auxin, gibberellins, cytokinins, brassinosteroids and abscisic acid, ...
[78]
https://www.researchgate.net/publication/391667004_Alteration_of_Source-Sink_Dynamics_and_Antioxidant_Responses_in_Potato_Solanum_tuberosum_L_Under_Potato_Leafroll_Virus_PLRV_Infection
[79]
Integrated Management of PLRV and PVY in Seed Potato, with ...
Symptoms of PLRV in potato include reddening and brittleness of leaves, stunting, necrosis, and characteristic rolling or cupping of foliage. PVY is the type ...
[80]
NAC: CHAPTER 587 - AGRICULTURAL PRODUCTS, SEEDS AND ...
... certified seed potatoes unless the Department approves certification. ... Potato leaf roll virus. 0.00. 0.00. 0.03. 0.05. 0.10. 0.20. Well-defined mosaic.
[81]
Disease Control - New England Vegetable Management Guide
PVX is not known to be spread by aphids but is easily spread by plant-to-plant contact, farm machinery, or cultural practices. Purchase certified seed to limit ...Missing: agronomic | Show results with:agronomic
[82]
OUTSMART APHIDS AND VIRUS - British Potato
May 15, 2025 · “Predictions of early aphid flights in Scotland are a concern, but a favourable spring means that crops should be in the ground early in ...
[83]
Overview of Aphids - PotatoPro
Trap Crops: Plant attractive crops like mustard or radish near potatoes to draw aphids away, then treat or remove the trap crops to reduce main crop infestation ...Aphid Biology · Natural Enemies Of Aphids · Cultural Control
[84]
Aphids / Potato / Agriculture: Pest Management Guidelines ... - UC IPM
Potato leafroll virus is spread by both aphids, but green peach aphid is by far the more effective vector. Early season leafroll infection stunts the plant.
[85]
[PDF] Insecticide Schedule for Potatoes
Remarks: Insecticide Resistance Group 4A. Clothianidin may be applied in-furrow or as a side-dress treatment for aphids, Colorado potato beetles, flea beetles ...
[86]
[PDF] 2015 Integrated Pest Management Guidelines for Insects and Mites ...
This, and the fact that most PVY transmission is by aphids that are not living in the potato field, means that PVY control with insecticides is poor at best. ...
[87]
Gotcha! Tiny Lady Beetles Have Big Biocontrol Potential
“Even before reaching adulthood, one beetle can eat 300 aphids,” Schaefer says. Its dinner menu can include more than 50 species of aphids and other soft-bodied ...
[88]
Potato Aphid : Vegetable : Center for Agriculture, Food, and the ...
Aug 27, 2020 · In general, aphid predators are better at controlling high aphid populations, compared to parasitoids ... Banker plants used to support Aphidius ...
[89]
Biological Control of Aphid Using Fungal Culture and Culture ... - NIH
Among 47 culture filtrates cultured potato dextrose broth, filtrate of Beauveria bassiana Bb08 showed the highest mortality (78%) against green peach aphid ...
[90]
Characterization of the expression and inheritance of potato leafroll ...
The PLRV resistance contributed by S. etuberosum is stably transmitted and expressed through sexual generations and therefore would be useful to potato ...Missing: Ry | Show results with:Ry
[91]
Mapping of the potato leafroll virus resistance gene, Rlr etb , from ...
Gene Rlr etb , derived from the potato species Solanum etuberosum, confers resistance to potato leafroll virus (PLRV). Mapping of this gene would aid in ...
[92]
Rladg: Genes List | GM Approval Database - ISAAA.org
Gene: Rladg. Gene Source: Product: Function: controls resistance to the infection and to PLRV accumulation. GM Trait: Viral disease resistance.
[93]
(PDF) Introgression of the Rladg allele of resistance to potato leafroll ...
Aug 8, 2025 · Genetic resistance to Potato Leafroll Virus (PLRV) is polygenic, which hinders the obtainment of resistant cultivars.
[94]
[PDF] Michigan State University - usda aphis
Apr 24, 2024 · Potato leafroll virus (PLRV) is a widespread and damaging disease of potato. The transmission is through aphids and through seed tubers ...Missing: symptoms | Show results with:symptoms
[95]
https://www.scielo.br/j/cbab/a/QpHLZb6WWxbRpzn6NBkzkKc/?lang=en
[96]
A major quantitative trait locus for resistance to Potato leafroll virus is ...
The major QTL, PLRV.1, mapped to potato chromosome XI in a resistance hotspot containing several genes for qualitative and quantitative resistance to viruses ...
[97]
A Major Quantitative Trait Locus for Resistance to Potato leafroll ...
The major QTL, PLRV.1, mapped to potato chromosome XI in a resistance hotspot containing several genes for qualitative and quantitative resistance to viruses ...
[98]
Strategies for Engineering Virus Resistance in Potato - MDPI
Apr 22, 2023 · In this study, we summarize the progress of antiviral strategies applied in potato through engineering either virus-derived or plant-derived genes.
[99]
Inhibition of potato leafroll virus multiplication and systemic ... - Nature
Dec 16, 2020 · RT-PCR for the actin gene was also performed simultaneously as an internal control (Fig. 4D). ELISA to detect PLRV in host plants. The presence ...
[100]
Marker-free PLRV resistant potato mediated by Cre-loxP excision ...
Aug 20, 2016 · Marker-free PLRV resistant potato mediated by Cre-loxP excision and RNAi ... The regeneration efficiency was thus 53 % and transformation ...Missing: efficacy | Show results with:efficacy
[101]
Full article: RNA Interference Approaches for Plant Disease Control
The authors estimated that if 80% of the potatoes grown in the region had RNAi-based resistance to PLRV, this could result in a reduction of approximately 1.8 ...
[102]
CRISPR/Cas9-Mediated Targeting of Susceptibility Factor eIF4E ...
Jul 12, 2022 · The CRISPR/Cas9 technology was used to create resistance to PVY, and resistant plants produced virus-free tubers.
[103]
Strategies for Engineering Virus Resistance in Potato - PMC
Apr 22, 2023 · RDR6-dependent RNA silencing pathways ... Moreover, viral resistance could be obtained through the CRISPR/Cas9 editing of plant endogenous genes.
[104]
Disease-resistant potato| GM Approval Database- ISAAA.org
Aug 15, 2024 · Rladg, controls resistance to the infection and to PLRV accumulation. Rysto, Solanum stoloniferum, Rysto immune receptor, confers extreme ...<|separator|>
[105]
Resistance to biotic and abiotic stress in potato
Jan 18, 2024 · The Ry-fsto gene from Solanum stoloniferum for extreme resistance to Potato virus Y maps to potato chromosome XII and is diagnosed by PCR ...