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Type II collagen

Type II collagen is a fibrillar protein that serves as the predominant structural component of hyaline cartilage in vertebrates, forming a robust network of fibrils that imparts tensile strength and resilience to tissues subjected to compressive forces.^[1] It consists of a homotrimer composed of three identical α1(II) polypeptide chains, each approximately 1,060 amino acids long, encoded by the COL2A1 gene on chromosome 12q13.11, and assembles into a characteristic triple-helical structure stabilized by repeating Gly-X-Y motifs where X is often proline and Y is hydroxyproline.^[2] This rod-like molecule, about 300 nm in length and 1.5 nm in diameter, undergoes post-translational modifications including hydroxylation and glycosylation before extracellular assembly into 50-nm-diameter fibrils with a 67-nm D-periodic banding pattern.^[3] Expressed primarily by chondrocytes, type II collagen is synthesized as a procollagen precursor that is cleaved to form mature fibrils, which integrate with minor collagens such as types IX and XI to create a heterogeneous cartilage matrix.^[3] It is the main collagenous element in articular cartilage, the notochord, intervertebral discs, and the vitreous humor of the eye, where it supports skeletal development, endochondral ossification, and vitreous gel stability.^[2] Interactions with proteoglycans like aggrecan and small leucine-rich proteoglycans such as decorin further enhance its role in load-bearing and hydration retention, preventing tissue deformation under physiological stress.^[1] Mutations in COL2A1, including missense variants and exon skipping, disrupt collagen folding, fibril assembly, or cross-linking, leading to a spectrum of heritable disorders known as type II collagenopathies.^[4] These range from lethal chondrodysplasias like achondrogenesis type II, characterized by severe skeletal hypoplasia and perinatal death, to non-lethal conditions such as Stickler syndrome, which features arthropathy, vitreoretinal degeneration, and sensorineural hearing loss.^[5] Additionally, COL2A1 variants contribute to osteoarthritis susceptibility and familial osteonecrosis of the femoral head by impairing cartilage homeostasis and vascular integrity.^[6]

Molecular Structure

Primary Sequence and Gene Product

Type II collagen is a homotrimeric fibrillar collagen composed of three identical α1(II) chains, each encoded by the COL2A1 gene located on the long arm of human chromosome 12 at locus 12q13.11.^[7] The gene spans approximately 31 kb and consists of 54 exons, which collectively encode the precursor protein known as pro-α1(II) chain, comprising 1,487 amino acids.^[8] This prochain serves as the primary gene product and represents the foundational linear polypeptide sequence prior to assembly and processing. The primary sequence of the pro-α1(II) chain is organized into distinct domains that define its structural and functional potential. At the N-terminus, a signal peptide of 23 amino acids directs the nascent polypeptide into the endoplasmic reticulum for secretion, after which it is cleaved to yield the mature pro-α1(II) chain.^[9] This is followed by a large N-propeptide of approximately 208 amino acids, which includes a von Willebrand factor type C (vWFC)-like domain implicated in regulating fibril nucleation during extracellular assembly. The central domain, spanning 1,014 amino acids, forms the signature triple helical region characterized by repeating Gly-X-Y triplets, where glycine occupies every third position—essential for tight packing of the three chains in the helix—and X and Y positions are predominantly proline residues, conferring rigidity to the structure. Interruptions in the Gly-X-Y repeats, more frequent in type II collagen than in other fibrillar collagens, introduce flexibility particularly suited to the dynamic fibrils of cartilage. The C-terminus features a globular C-propeptide of about 242 amino acids, which facilitates initial association and selection of three identical α1(II) chains during trimer formation.^[9]^[5] The primary sequence of type II collagen exhibits remarkable evolutionary conservation across vertebrates, reflecting its critical role in skeletal development. In the triple helical domain, human and mouse sequences share approximately 95% amino acid identity, with only about 37 substitutions across the 1,014 residues, underscoring the functional constraints on this region.^[10] This high conservation extends to other vertebrates, ensuring the structural integrity necessary for cartilage formation and maintenance.

Triple Helix Formation

Type II collagen forms a homotrimer composed of three identical pro-α1(II) chains, which are initially synthesized as procollagen molecules with N- and C-terminal propeptides.^[11] The assembly into the procollagen trimer begins intracellularly through the association of the C-propeptides, which nucleate the formation of the triple helix by bringing the three chains into close proximity and aligning them in register.^[12] The triple helix nucleation occurs at the C-terminus, where sequences rich in imino acids (proline and hydroxyproline) provide enhanced stability to initiate folding.^[12] From this nucleation site, the helix propagates toward the N-terminus in a zipper-like manner, with an in vitro propagation rate of approximately 100 residues per second at physiological temperatures.^[13] This directional folding ensures the correct alignment of the Gly-X-Y repeats across the three chains. The mature triple helix exhibits a right-handed superhelical structure with a pitch of 0.86 nm per turn (corresponding to three residues) and a diameter of approximately 1.5 nm.^[12] Its stability is maintained by direct interchain hydrogen bonds, primarily between the glycine NH group of one chain and the carbonyl oxygen of proline in the X position of an adjacent chain, supplemented by water-mediated bridges involving hydroxyproline residues in the Y position.^[12] The thermal stability of the type II collagen triple helix has a melting temperature (Tm) of about 41°C, which is slightly lower than that of type I collagen (Tm ≈ 42°C) due to a modestly reduced content of stabilizing imino acids (proline + hydroxyproline ≈ 21% in type II versus 23% in type I).^[12] This lower Tm confers greater flexibility to type II collagen, facilitating its role in dynamic tissues like cartilage under mechanical stress.^[12] Mutations substituting glycine residues in the Gly-X-Y repeats disrupt the close packing required for hydrogen bonding, leading to delayed helix propagation and overmodification of the chains during biosynthesis.^[11] Such defects, including glycine-to-arginine substitutions in the COL2A1 gene, compromise overall helix integrity and are associated with connective tissue disorders (as detailed in the Pathology section).^[11]

Higher-Order Assembly

Type II collagen, synthesized as procollagen with N- and C-terminal propeptides, undergoes extracellular processing to enable higher-order assembly into fibrils. The N-propeptide is cleaved by ADAMTS-2, a metalloproteinase specific for fibrillar procollagens including type II, while the C-propeptide is removed by BMP-1 (also known as procollagen C-proteinase), yielding the mature collagen molecule competent for fibrillogenesis.^[14]89822-6/fulltext) This cleavage is essential, as the propeptides sterically hinder premature aggregation during secretion. Fibril nucleation begins with the lateral association of mature type II collagen molecules, where the N-terminal region of one molecule interacts with the C-terminal region of an adjacent molecule, mediated initially by residual N-propeptide interactions in some models before full processing. These molecules align in a staggered array, defining the characteristic D-period of approximately 67 nm, which arises from the axial offset and produces the banded appearance observed in electron microscopy.^[15]^[2] In cartilage, the resulting fibrils are initially thin, with diameters of 15-20 nm, and subsequently aggregate laterally into thicker fibers (up to 50 nm or more) to form hierarchical structures capable of bearing mechanical loads. Stability is conferred by enzymatic cross-linking via lysyl oxidase, which oxidizes lysine residues to aldehydes that condense into covalent bonds such as dehydrohydroxylysinonorleucine, enhancing fibril tensile strength.^[16]^[15]^[17] The quarter-stagger model governs this assembly, with molecules overlapping by about 40% of their length (approximately 40 nm) and leaving a gap region for accommodation of non-collagenous matrix components. This arrangement repeats every D-period, optimizing both structural integrity and tissue-specific functionality.^[15] Tissue-specific adaptations in fibril assembly are evident in the vitreous humor, where type II collagen forms uniform, thin fibrils (10-12 nm diameter) that lack extensive cross-linking, maintaining optical transparency and flexibility essential for the gel-like vitreous structure.^[15]

Biosynthesis and Processing

Transcription and Translation

The transcription of the COL2A1 gene, which encodes the alpha-1 chain of type II collagen, is primarily regulated in chondrocytes through promoter elements responsive to the transcription factor SOX9, a master regulator of chondrogenesis. SOX9 binds directly to a conserved enhancer sequence within intron 1 of COL2A1, activating tissue-specific expression in cartilaginous tissues.^[18] Additionally, a distal enhancer in intron 6 cooperates with SOX9, SOX5, and SOX6 to further enhance transcription, ensuring high-level expression during chondrocyte differentiation.^[19] These regulatory elements collectively drive chondrocyte-specific COL2A1 transcription, with SOX9/SOX5/SOX6 forming a complex that binds multiple sites across the gene locus.^[20] Following transcription, COL2A1 pre-mRNA undergoes alternative splicing, particularly of exon 2, to produce distinct isoforms tailored to developmental stages. The type IIA isoform, which retains exon 2, predominates in chondroprogenitor cells and embryonic cartilage, while the type IIB isoform, lacking exon 2, is the major form in differentiated adult chondrocytes.^[21] This splicing event is developmentally regulated, with exon 2 inclusion decreasing as cells mature, thereby modulating the protein's function in early versus mature cartilage matrix assembly.^[22] Translation of COL2A1 mRNA occurs on ribosomes associated with the rough endoplasmic reticulum (ER), where the nascent polypeptide is co-translationally translocated into the ER lumen via an N-terminal signal peptide that is subsequently cleaved.^[23] In the ER, chaperone proteins such as BiP (also known as GRP78) bind to the emerging chain, preventing aggregation and assisting in initial folding prior to triple helix formation.^[24] This process ensures efficient synthesis of the pro-alpha1(II) chain, which constitutes a major secretory product in chondrocytes. COL2A1 expression is notably high in hypertrophic chondrocytes during endochondral ossification, supporting cartilage matrix production in the growth plate, while it is downregulated in osteoblasts that favor type I collagen synthesis.^[25] Quantitatively, COL2A1 mRNA represents a substantial portion of the chondrocyte transcriptome, underscoring its dominance in the cellular output dedicated to extracellular matrix components.^[23]

Post-Translational Modifications

Post-translational modifications of type II collagen occur primarily in the endoplasmic reticulum (ER) following translation of the procollagen chains, ensuring proper folding, stability, and secretion in the hypoxic environment of cartilage. These modifications are essential for the protein's structural integrity, as type II collagen relies on them to form a stable triple helix and interact with other extracellular matrix components. Unlike the unmodified translation product, which is prone to degradation, these enzymatic alterations adapt the collagen to the low-oxygen conditions prevalent in avascular cartilage tissues.^[26] Hydroxylation is a key modification, where prolyl 4-hydroxylase (P4H) and lysyl hydroxylase (LH, also known as PLOD) enzymes add hydroxyl groups to proline and lysine residues, respectively. This process requires ascorbic acid as a cofactor and occurs co-translationally in the ER, stabilizing the triple helix through hydrogen bonding and facilitating secretion. Under hypoxic conditions in cartilage, hypoxia-inducible factor-1α (HIF-1α) upregulates P4H and LH expression, maintaining efficient hydroxylation despite limited oxygen availability, which is critical for chondrocyte survival and matrix deposition.^[26]^[27] Glycosylation follows hydroxylation, primarily targeting hydroxylysine residues to form galactosyl-hydroxylysine (Gal-Hyl) or glucosyl-galactosyl-hydroxylysine (Glc-Gal-Hyl) disaccharides. These modifications are catalyzed by collagen galactosyltransferase (e.g., COLGALT1/2) and glucosyltransferase (e.g., B4GALT7 or LH3), adding β-D-galactose and α-D-glucose units that influence fibril diameter, packing, and resistance to proteolysis. In type II collagen, higher glycosylation levels compared to type I contribute to the loose, arcading fibril architecture essential for cartilage's compressive properties.^[28] Additional modifications include limited sulfation of tyrosine residues and formation of disulfide bonds within the C-propeptide, which promote trimerization and proper alignment during helix assembly. Sulfation is minimal in type II collagen, unlike in other collagens, to avoid excessive charge that could disrupt fibril interactions. These intracellular changes occur before secretion, with disulfide isomerases ensuring correct bonding for stability.^[29] In pathological conditions, such as chondrodysplasias caused by COL2A1 mutations, delayed triple helix formation allows prolonged exposure to hydroxylases, resulting in hyperhydroxylation and over-glycosylation. This overmodification alters electrophoretic mobility, enabling detection via techniques like SDS-PAGE, where overmodified chains migrate more slowly due to increased negative charge from added sugar moieties. Such changes compromise collagen function, leading to skeletal abnormalities.^[30] ER quality control mechanisms degrade unmodified or improperly modified procollagen chains to prevent accumulation of dysfunctional proteins. Unhydroxylated chains fail to fold correctly and are recognized by chaperones like calnexin, targeting them for ER-associated degradation (ERAD) via retrotranslocation to the cytosol for proteasomal breakdown or selective ER-phagy involving FAM134B receptors. In type II collagen biosynthesis, this pathway ensures only properly modified trimers proceed to secretion, with defects exacerbating ER stress in cartilage disorders.^[31]

Secretion and Fibril Formation

Following its post-translational modifications in the endoplasmic reticulum (ER), type II procollagen is transported to the Golgi apparatus via coat protein complex II (COPII)-coated vesicular tubular clusters, a process that segregates it from other extracellular matrix components in chondrocytes.^[32] Within the Golgi, procollagen molecules aggregate into higher-order structures within the cisternae, causing distensions that facilitate cisternal maturation and progression through the Golgi stack without luminal exit.^[32] This aggregation prepares the procollagen for secretion via large Golgi-to-plasma membrane carriers, which may directly fuse with the cell surface or form specialized fibripositors to support extracellular deposition.^[32] Type II procollagen is secreted through a constitutive pathway in chondrocytes, bypassing regulated secretory granules typical of some extracellular matrix proteins.^[3] The solubility of procollagen is maintained intracellularly by the acidic pH of the ER and Golgi (approximately pH 6.5–7.0), which prevents premature triple helix interactions and fibril nucleation; upon release into the neutral extracellular environment (pH ~7.4), solubility decreases, priming the molecules for assembly once propeptides are removed.^[3] This pH gradient ensures controlled export without intracellular precipitation. In the extracellular space, the N-terminal propeptide of type II procollagen is primarily cleaved by ADAMTS-3, a metalloproteinase that recognizes a specific site between the short N-terminal domain and the central collagenous region, while the C-propeptide is processed by members of the tolloid family (e.g., BMP-1).^[33] This cleavage occurs at neutral pH and is essential for triggering self-assembly, as the intact propeptides sterically hinder intermolecular interactions; incomplete processing leads to disrupted fibril formation observed in certain collagenopathies.^[33] Initial fibrillogenesis of type II collagen is highly dependent on environmental factors, including neutral pH (optimal range 7.0–8.5) and physiological ionic strength (0.15–0.4 M NaCl), which promote nucleation and lateral growth into thin fibrils (typically 20–50 nm in diameter) characteristic of cartilage.^[34] In cartilage, this process is nucleated and regulated by cartilage oligomeric matrix protein (COMP), a pentameric thrombospondin that binds type II collagen with high affinity (Kd ≈ 1.7 nM) via its C-terminal domain, bridging molecules to accelerate assembly and enhance fibril stability.^[35] Fibronectin contributes supportively by organizing the pericellular matrix through integrin-mediated interactions, localizing type II collagen deposition near the chondrocyte surface without directly nucleating fibrils.^[36] Compared to type I collagen, fibril assembly of type II is notably slower, with a lag phase 5–6 times longer and a propagation rate approximately 30 times lower following propeptide removal, attributed to its lower density of intermolecular cross-links and adaptations for the compressive environment of cartilage.^[37] This tempered kinetics allows for finer control over fibril diameter and integration with minor collagens like type XI, ensuring the uniform, heterotypic architecture of hyaline cartilage fibrils.^[37]

Tissue Distribution and Function

Role in Cartilage

Type II collagen constitutes approximately 50-80% of the dry weight of articular cartilage and 90-95% of its total collagen content, forming the primary structural framework of the extracellular matrix.^[38]^[39] This fibrillar network provides essential tensile strength, enabling the tissue to withstand multidirectional mechanical loads during joint movement. The collagen fibrils, organized in a characteristic Benninghoff arcade-like structure, resist tensile forces while maintaining tissue integrity under shear and compressive stresses.^[40]^[41] In addition to tensile support, type II collagen fibrils play a critical role in entrapping proteoglycans, particularly aggrecan, within the matrix. This entrapment creates a hydrated gel-like environment that confers compressive resistance to cartilage, as the negatively charged proteoglycans attract and retain water molecules, contributing to the tissue's high water content of 65-80%.^[42]^[40] The arcade arrangement of the fibrils forms a porous network that confines these aggregates, preventing their diffusion while allowing fluid flow for nutrient exchange and load dissipation. In growth plate cartilage, similar fibrillar organization supports endochondral ossification by providing structural stability during transient compressive loads.^[43] Zonal variations in type II collagen fibril organization and diameter further optimize cartilage function across its depth. In the superficial zone, thin fibrils (approximately 20-50 nm in diameter) align parallel to the articular surface, enhancing resistance to shear forces and surface wear. Deeper zones feature progressively thicker fibrils (up to 100 nm), oriented radially to anchor the tissue to subchondral bone and bolster compressive strength. These adaptations ensure biomechanical heterogeneity, with the overall fibril network exhibiting a Young's modulus on the order of 1 MPa in the superficial zone, contributing to the tissue's ability to retain up to 80% water under load.^[44]^[45] Metabolic turnover of type II collagen in adult articular cartilage is notably slow, with a half-life estimated at 100-400 years, reflecting the tissue's avascular nature and low cellularity. This longevity preserves the structural integrity of the matrix over decades, minimizing degradation under normal physiological conditions. However, during injury or repair processes, such as in fracture healing or osteoarthritis initiation, turnover accelerates via upregulated matrix metalloproteinase activity, facilitating remodeling but risking fibril disruption if unbalanced. In growth plate cartilage, turnover is more dynamic to accommodate rapid skeletal elongation.^[46]^[47]

Presence in Other Tissues

Although type II collagen is predominantly found in cartilage, it is also present in several other tissues where it contributes to specialized extracellular matrices.^[48] In the vitreous humor of the eye, type II collagen forms the primary structural component of a gel-like matrix, accounting for 60-80% of the total collagen content. It assembles into uniform, thin fibrils that interact with proteoglycans such as opticin, which binds to these fibrils and helps regulate their spacing to maintain ocular transparency. These fibrils, often co-expressed with minor amounts of type XI collagen, provide tensile strength while allowing light transmission.^[49]^[50]^[51] Within the intervertebral disc, type II collagen is concentrated in the nucleus pulposus, where it constitutes the predominant collagen type and forms an irregular network that supports proteoglycan aggregation and tissue hydration, akin to its role in cartilaginous structures. This composition enables the nucleus to withstand compressive forces and maintain disc integrity. Type XI collagen is co-expressed here at low levels to regulate fibril assembly.^[52]^[53]^[54] In the cochlea, type II collagen is a major constituent of the tectorial membrane, comprising a significant portion of its protein content alongside types IX and XI, forming a gelatinous overlay on the organ of Corti. These collagen fibrils, cross-linked by tectorins, facilitate the mechanical coupling of sound-induced vibrations from the basilar membrane to hair cell stereocilia, essential for auditory transduction.^[55]^[56] During development, type II collagen exhibits transient expression in the notochord, where it supports axial structure formation, and in the perichondrium surrounding nascent cartilage anlagen, aiding early chondrogenesis before shifting to more permanent sites. In these non-cartilaginous contexts, type II collagen typically represents a minor fraction of total tissue collagen, often less than 5% overall, but plays critical regulatory roles when co-expressed with type XI.^[57]^[58]^[59]^[54]

Interactions with Extracellular Matrix Components

Type II collagen engages in electrostatic interactions with the aggrecan core protein, primarily through basic residues on the collagen that attract the negatively charged glycosaminoglycan chains of aggrecan, facilitating adhesion within the cartilage matrix.^[60] These interactions are modulated by ionic strength, underscoring their electrostatic nature and role in maintaining matrix integrity under physiological conditions.^[61] Additionally, the keratan sulfate-rich region of aggrecan binds to type II collagen fibrils via the core protein, further stabilizing the proteoglycan-collagen network.^[62] In heterotypic fibril formation, type II collagen co-assembles with type XI collagen, where type XI acts as a nucleator and regulator of fibril diameter, ensuring uniform thin fibrils characteristic of cartilage.^[63] Type XI collagen integrates into the core of these fibrils during early assembly stages, influencing the overall microstructure through intermolecular cross-linking.^[64] Complementing this, type IX collagen associates peripherally with type II/XI heterotypic fibrils, with its NC4 domain providing surface anchoring that positions the proteoglycan side chains outward for interactions with other matrix components.^[65] Type II collagen binds to integrins α1β1 and α2β1 on chondrocytes, serving as adhesion receptors that transduce mechanical and biochemical signals to regulate cell behavior and matrix homeostasis.^[66] These interactions promote chondrocyte attachment to the collagen network, with α1β1 showing preference for type VI collagen but also supporting type II binding, while α2β1 exhibits stronger affinity for type II collagen, facilitating signaling pathways that influence proliferation and differentiation.^[67] Through these receptors, type II collagen modulates intracellular pathways, including those involved in cytoskeletal organization and response to environmental cues in the extracellular matrix.^[68] Cleavage products of type II collagen, known as matricryptins, exhibit bioactive properties that modulate inflammation in the cartilage microenvironment. For instance, cyanogen bromide-generated peptides such as CB12-II (residues 195–218) upregulate matrix metalloproteinase-13 (MMP-13) expression and induce further collagen degradation, amplifying inflammatory responses in arthritic conditions.^[69] Other collagenase-derived fragments, like the 3/4 C-terminal piece, act as matrikines by stimulating cytokine release and immune cell activation, contributing to pathological remodeling.^[70] In pathological contexts, type II collagen demonstrates sensitivity to enzymatic degradation and pH variations, which exacerbate matrix breakdown. MMP-13 preferentially cleaves the triple helix at the Gly775-Ile776 bond, initiating fibril unraveling and subsequent fragmentation in osteoarthritis.^[71] ADAMTS-5, while primarily targeting aggrecan, contributes to overall matrix disassembly by facilitating access for collagenases, with its activity heightened in inflammatory environments.^[72] Acidic pH conditions, common in inflamed joints, enhance collagenase efficiency and fibril swelling, promoting denaturation and increased susceptibility to proteolysis.^[73]

Developmental Biology

Expression During Embryogenesis

Type II collagen expression initiates shortly after gastrulation in the prechondrogenic mesoderm of vertebrate embryos, marking the onset of chondrogenic commitment. In mouse embryos, Col2a1 transcripts first appear at embryonic day (E) 9.5 in the sclerotome of somites and cranial mesenchyme, regions destined to form cartilage elements of the axial and appendicular skeleton.^[58] This early expression occurs in mesenchymal cells prior to overt differentiation, with low levels of type IIA procollagen isoform detected in these prechondrogenic populations.^[74] As development proceeds, mesenchymal cells condense to form precartilaginous templates, where the type IIA isoform predominates in chondroprogenitor cells within these condensations.^[74] The transition to differentiated chondrocytes coincides with cavitation of these condensations and a switch to the type IIB isoform, which lacks exon 2 and supports mature cartilage matrix assembly.^[74] This isoform switch is orchestrated by the SOX9/SOX5/SOX6 transcription factor trio, which cooperatively activates Col2a1 expression in a stage-specific manner to drive the mesenchymal-to-chondrocyte transition.^[75] In developing limb buds, Col2a1 expression exhibits a proximal-to-distal gradient, beginning in proximal mesenchymal condensations around E10.5 in mice and extending distally as chondrogenesis progresses, ensuring sequential cartilage formation along the limb axis.^[76] This pattern is critical for proper limb morphogenesis, as type II collagen provides structural support during the differentiation process. Beyond skeletal tissues, type II collagen is expressed transiently in non-chondrogenic sites derived from neural crest cells, particularly in ocular structures such as the neural retina, corneal epithelium, and sclera, where it appears by E10.5 in mice and contributes to early tissue patterning.^[58] Additionally, transient expression occurs in the embryonic heart, including the epicardium, myocardium, and developing valve regions akin to septa, from E9.5 to E12.5, before diminishing as cardiac structures mature. Disruption of Col2a1 in knockout mice reveals its essential role, with no overt defects until E12.5, after which embryos exhibit severe dwarfism due to shortened long bones lacking growth plates, enlarged vertebral bodies, and cleft palate, leading to perinatal lethality.^[57] These phenotypes underscore the protein's necessity for notochord regression and proper cartilage template formation during embryogenesis.

Involvement in Skeletal Development

Type II collagen serves as the primary structural protein in the cartilage template during endochondral ossification, the process responsible for long bone growth and the replacement of cartilaginous models with bone tissue. In the growth plate, it is abundantly expressed in the proliferative and pre-hypertrophic zones, where it forms a robust fibrillar network that supports chondrocyte proliferation, organization into columns, and the synthesis of extracellular matrix components essential for longitudinal elongation. This scaffold provides mechanical stability and facilitates nutrient diffusion, enabling sustained bone growth throughout development.^[77]^[43]^[78] As chondrocytes transition to hypertrophy, type II collagen expression is downregulated, coinciding with the upregulation of type X collagen in the hypertrophic zone, which promotes matrix mineralization and remodeling to accommodate vascular invasion and osteoblast activity. This shift is critical for the progression of endochondral ossification, as the degradation and reorganization of type II collagen fibrils create pathways for blood vessels and bone-forming cells to replace cartilage. Disruptions in this transition, such as impaired collagenolysis, delay ossification and lead to skeletal abnormalities.^[79]^[43]^[80] In joint formation, type II collagen maintains the integrity of the synovial cavity by forming the articular cartilage layer, which ensures proper separation of skeletal elements and prevents fusion; defects in its assembly result in joint malformations observed in collagenopathies. Similarly, in craniofacial development, it is integral to the mandibular condyle and Meckel's cartilage, where it supports secondary cartilage growth and the morphogenesis of jaw structures through endochondral processes.^[4]^[81]^[82] The expression of type II collagen in these contexts is synergistically induced by growth factors such as BMP-2 and TGF-β, which activate signaling pathways that enhance chondrogenesis and matrix production, thereby driving longitudinal bone elongation and overall skeletal patterning. BMP-2, in particular, upregulates type II collagen synthesis in chondrocytes, while TGF-β promotes its deposition in the extracellular matrix to sustain proliferative activity.^[83]^[84]^[85]

Regulation of Chondrogenesis

Chondrogenesis, the process of cartilage formation during skeletal development, is tightly regulated by molecular pathways that control the expression of Type II collagen (encoded by COL2A1) in differentiating chondrocytes. These pathways ensure precise spatiotemporal control of COL2A1 transcription, enabling the transition from mesenchymal precursors to mature cartilage tissue. Key transcription factors and signaling cascades integrate extracellular cues to activate or repress COL2A1, maintaining chondrocyte identity while preventing aberrant differentiation. The SOX trio of transcription factors—SOX9, SOX5, and SOX6—plays a central role in activating COL2A1 expression during early chondrogenesis. SOX9 binds directly to enhancer elements within the COL2A1 gene, initiating transcription in chondroprogenitor cells. SOX5 and SOX6 act as co-activators, cooperating with SOX9 to amplify this activation by binding adjacent sites on the enhancer, which leads to robust upregulation of COL2A1 and other cartilage matrix genes. This cooperative mechanism is essential for mesenchymal condensation and the establishment of the chondrogenic phenotype, as demonstrated in studies using mouse models where disruption of the SOX trio impairs cartilage formation. The Wnt/β-catenin pathway exerts inhibitory effects on COL2A1 expression in specific regions, such as the periarticular interzones that define future joint spaces. Activation of canonical Wnt signaling stabilizes β-catenin, which translocates to the nucleus and suppresses chondrogenic differentiation by repressing SOX9 activity and directly inhibiting COL2A1 promoter elements. This localized inhibition prevents cartilage formation in joint regions, promoting synovial joint specification instead. Experimental evidence from conditional β-catenin gain-of-function models shows reduced COL2A1 expression and ectopic joint-like structures, underscoring the pathway's role in delineating skeletal boundaries. Hedgehog signaling, particularly through Indian hedgehog (Ihh), modulates COL2A1 indirectly in the hypertrophic zones of the growth plate. Ihh is upregulated in prehypertrophic chondrocytes, where it promotes hypertrophy by coordinating with parathyroid hormone-related protein (PTHrP) to regulate proliferation and maturation. During this transition, Ihh signaling contributes to the downregulation of COL2A1 expression, shifting the chondrocyte phenotype toward matrix mineralization and vascular invasion. In vitro and in vivo studies reveal that enhanced Ihh activity accelerates hypertrophy while diminishing COL2A1 levels, highlighting its role in terminating the cartilaginous phase. Epigenetic mechanisms further fine-tune COL2A1 expression during chondrocyte differentiation and maintenance. Histone acetylation at the COL2A1 promoter, mediated by co-activators like p300/CBP recruited by SOX9, opens chromatin structure to facilitate transcription and enhance chondrogenic gene accessibility. Conversely, microRNA-29b (miR-29b) represses COL2A1 in dedifferentiating chondrocytes by targeting its 3'-untranslated region, leading to reduced mRNA stability and protein levels. This repression is prominent in conditions mimicking loss of chondrocyte phenotype, where miR-29b overexpression correlates with decreased COL2A1 and increased fibrotic markers. Feedback loops involving Runx2 provide a mechanism to repress COL2A1 and direct the switch from chondrogenesis to osteogenesis. Runx2, a master regulator of hypertrophy, binds regulatory elements to suppress COL2A1 transcription in late-stage chondrocytes, favoring osteogenic gene programs. This repression is part of a cross-inhibitory network with SOX9, where elevated Runx2 levels promote matrix degradation and terminal differentiation. Genetic models overexpressing Runx2 in chondrocytes demonstrate accelerated COL2A1 downregulation and premature ossification, illustrating the loop's importance in lineage commitment.

Pathology and Disorders

Genetic Mutations and Collagenopathies

Mutations in the COL2A1 gene, which encodes the alpha-1 chain of type II collagen, are responsible for a spectrum of autosomal dominant disorders collectively known as type II collagenopathies. These inherited conditions primarily affect skeletal development, leading to a range of phenotypes from perinatal lethality to milder joint and ocular abnormalities. The majority of pathogenic variants occur in the coding region for the triple helical domain, disrupting the Gly-X-Y repeat sequence essential for collagen fibril assembly. Inheritance is typically autosomal dominant, with de novo mutations accounting for many cases, and the exact prevalence of severe forms, such as achondrogenesis type II and spondyloepiphyseal dysplasia congenita, is unknown, though they are rare genetic conditions with estimates around 1:100,000 for SEDC and rarer for achondrogenesis type II.^[4]^[5] The most common mutation types are glycine substitutions within the triple helical domain, which exert a dominant-negative effect by incorporating abnormal chains into collagen trimers, thereby impairing fibril formation. For instance, the G997S substitution (c.2989G>A) has been identified in individuals with spondyloepiphyseal dysplasia congenita, leading to severe short stature and skeletal deformities. Splice site mutations, such as those causing exon skipping (e.g., c.1091-2A>G), result in shortened or unstable transcripts, further contributing to reduced functional type II collagen. In contrast, null alleles like nonsense or frameshift variants cause haploinsufficiency, often associated with milder conditions such as Stickler syndrome.^[86]^[4]^[5] The spectrum of disorders includes lethal achondrogenesis type II, characterized by extreme micromelia and absent vertebral ossification due to dominant-negative missense mutations such as glycine substitutions; spondyloepiphyseal dysplasia congenita, featuring glycine-to-serine or arginine substitutions that cause progressive spinal and epiphyseal abnormalities; Stickler syndrome, where glycine disruptions lead to vitreoretinal degeneration, hearing loss, and arthropathy; and milder familial osteoarthritis from subtle helical domain alterations. Mechanistically, mutant chains cause misfolded trimers to be retained in the endoplasmic reticulum, activating the unfolded protein response and leading to chondrocyte stress, apoptosis, and reduced extracellular matrix fibril density. Genotype-phenotype correlations indicate that mutations in the N-terminal region of the triple helix tend to produce more severe phenotypes compared to those in the C-terminal region, which are often milder and associated with later-onset joint issues.^[4]^[86]^[87]

Role in Osteoarthritis and Degenerative Diseases

Type II collagen degradation plays a central role in the pathogenesis of osteoarthritis (OA), a degenerative joint disease characterized by progressive cartilage breakdown. In OA, the articular cartilage loses its structural integrity primarily due to enzymatic cleavage of type II collagen fibrils, which constitute over 90% of the collagen in cartilage and provide tensile strength. This degradation disrupts the extracellular matrix (ECM), leading to joint dysfunction and pain.^[88] Degradation of type II collagen in OA is predominantly mediated by matrix metalloproteinases (MMPs), particularly MMP-13 (collagenase-3), which preferentially cleaves the triple-helical structure at specific sites. MMP-13 initiates intra-helical cleavage at the Gly^{775}-Ile^{776} bond in the α1 chain and a corresponding Gly-Leu bond in the α2 chain, generating characteristic neoepitopes such as COL2-3/4C^{short}, which serve as biomarkers of active collagenolysis. These neoepitopes can be detected in cartilage extracts and synovial fluid from OA patients, reflecting increased collagenase activity compared to healthy tissue. Other MMPs, like MMP-1 and MMP-8, contribute but with lower efficiency toward type II collagen.^[89]^[88]^[90] The resulting collagen fragments compromise fibril integrity, initiating a cascade of ECM disruption. Cleaved fibrils lose their ability to encase proteoglycans like aggrecan, leading to rapid depletion of these hydrated molecules and reduced cartilage hydration and shock absorption. This exposes underlying bone and promotes aberrant mineralization, including ectopic calcification within the cartilage matrix, which further stiffens the tissue and exacerbates mechanical stress. In advanced OA, fragmented collagen accumulates in the superficial zone, correlating with fibrillation and erosion of the articular surface.^[91]^[92] Aging significantly alters type II collagen homeostasis, predisposing cartilage to degenerative changes. With advancing age, non-enzymatic glycation leads to increased advanced glycation end-products (AGEs), such as pentosidine, which form intermolecular cross-links between collagen molecules, reducing fibril elasticity and impairing biomechanical properties. Pentosidine levels rise progressively from age 20 onward, contributing to cartilage stiffening and vulnerability to mechanical damage. Additionally, type II collagen synthesis by chondrocytes declines sharply after age 40, with incorporation rates dropping to less than 2% of new protein in mature cartilage, while turnover remains low, amplifying the impact of degradative processes.^[93]^[94]^[95] Environmental and mechanical risk factors accelerate type II collagen turnover in OA. Obesity imposes excessive joint loading, elevating MMP-13 expression and collagen degradation rates through biomechanical stress and adipokine-mediated inflammation. Joint injury, such as anterior cruciate ligament tears, triggers acute collagen fibril disruption, increasing fragment release and subsequent catabolic signaling. These type II collagen fragments act as damage-associated molecular patterns, stimulating chondrocytes via Toll-like receptors to produce pro-inflammatory cytokines like IL-1β, which further upregulates MMPs and perpetuates degradation.^[96]^[97]^[98] Urinary C-terminal telopeptide of type II collagen (uCTX-II) emerges as a key progression marker for knee OA. Elevated uCTX-II levels indicate ongoing cartilage collagen breakdown and correlate with radiographic joint space narrowing and structural progression over 2-5 years. In cohorts of knee OA patients, baseline uCTX-II above 500 ng/mmol creatinine predicts a 2-3-fold higher risk of disease worsening, independent of age or BMI, making it a valuable prognostic tool for monitoring degenerative advancement.^[99]^[100]

Autoimmune and Inflammatory Conditions

Type II collagen (CII) acts as a key autoantigen in rheumatoid arthritis (RA), where T-cells recognize specific triple-helical epitopes, such as the arthritogenic sequence CII260-270, leading to an autoimmune response that drives joint inflammation.^[101] These T-cell responses are restricted by certain MHC class II molecules, like HLA-DR4, and contribute to the initiation of disease in susceptible individuals.^[102] Additionally, autoantibodies targeting native CII, rather than denatured forms, are prevalent and correlate with disease activity and symptom severity in RA patients.^[103] Breakdown of immune tolerance to CII is central to RA pathogenesis, particularly through disruption of oral tolerance mechanisms in the gut mucosa. Normally, ingestion of undenatured CII by gut-associated lymphoid tissue dendritic cells promotes the generation of regulatory T-cells (Tregs) that secrete inhibitory cytokines like TGF-β and IL-10, suppressing autoreactive responses systemically.^[104] In RA, this tolerance fails, allowing CII-specific T-cells to escape regulation and proliferate, exacerbating autoimmunity.^[104] In the synovial joint, proteolytic fragments of CII generated during inflammation act as neoantigens that amplify Th17 cell responses, promoting the production of proinflammatory cytokines such as IL-17 and IL-22, which drive chronic synovitis and pannus formation.^[102] This process leads to invasive synovial tissue that erodes cartilage and bone, perpetuating the autoimmune cycle in RA.^[105] CII autoimmunity is also implicated in relapsing polychondritis, a rare disorder where anti-Type II antibodies target cartilaginous structures, causing recurrent inflammation and progressive destruction of ear, nose, and tracheobronchial cartilage.^[106] These antibodies, often IgG class, bind native CII epitopes and activate complement, leading to tissue damage.^[107] The collagen-induced arthritis (CIA) model in mice replicates these mechanisms, where immunization with heterologous CII induces T- and B-cell responses mimicking human RA and relapsing polychondritis.^[108] Anti-CII antibodies are detected in approximately 20-40% of patients with early RA, with prevalence around 27% at disease onset, and their presence is associated with more aggressive progression and erosive joint damage.^[103]^[109]

Clinical and Therapeutic Aspects

Diagnostic Approaches

Genetic testing serves as the cornerstone for diagnosing type II collagen disorders, primarily through sequencing the COL2A1 gene, which encodes the alpha-1 chain of type II collagen. Sanger sequencing is employed to identify point mutations, such as missense, nonsense, and splice-site variants, and is recommended as an initial targeted approach for suspected cases based on clinical features like skeletal dysplasia or ocular abnormalities.^[4] Next-generation sequencing (NGS), often via multigene panels or whole-exome sequencing, enables broader detection including copy number variants and is particularly useful for atypical presentations or when mosaicism is suspected, occurring in 6-10% of parental cases.^[110] Variant pathogenicity is classified according to American College of Medical Genetics and Genomics (ACMG) guidelines, integrating evidence from population databases, computational predictions, functional studies, and segregation analysis to confirm deleterious effects.^[111] Biochemical assays provide non-invasive assessment of type II collagen turnover in cartilage, aiding in the evaluation of degenerative conditions associated with collagen abnormalities. Serum cartilage oligomeric matrix protein (COMP) levels reflect cartilage synthesis and degradation, with elevated concentrations indicating increased matrix remodeling.^[112] Urinary C-terminal telopeptide of type II collagen (CTX-II) measures collagen breakdown products, serving as a biomarker for cartilage degradation; higher levels correlate with disease progression in osteoarthritis and other collagen-related arthropathies.^[113] These assays, typically performed via enzyme-linked immunosorbent assay (ELISA), offer quantitative insights into matrix integrity but require correlation with clinical findings for specificity.^[114] Imaging modalities visualize structural disruptions in tissues rich in type II collagen, such as cartilage and vitreous, supporting clinical diagnosis. Magnetic resonance imaging (MRI) detects fibril disruption and cartilage thinning in joints, revealing early degenerative changes or dysplasia in conditions like spondyloepiphyseal dysplasia congenita. Ultrasound is valuable for prenatal screening in Stickler syndrome, identifying features like micrognathia, cleft palate, or vitreous anomalies, and for postnatal assessment of ocular or joint involvement.^[4] Radiographs complement these by showing skeletal irregularities, such as platyspondyly or epiphyseal abnormalities, though MRI provides superior soft tissue detail. Immunohistology through cartilage biopsy staining evaluates matrix composition and integrity at the tissue level. Biopsies from affected joints are stained with anti-type II collagen (anti-CII) antibodies to quantify collagen distribution and detect abnormalities like reduced fibril density or irregular deposition.^[115] This technique, often using immunofluorescence or immunohistochemistry, highlights disruptions in the extracellular matrix, confirming collagen defects in ambiguous cases.^[116] Such analyses are invasive and reserved for research-supported diagnostics where imaging is inconclusive.^[115] Functional tests in chondrocyte cultures assess the impact of COL2A1 mutations on collagen processing and secretion. Primary chondrocytes derived from patient biopsies are cultured to evaluate mutant protein effects, such as delayed triple helix formation or endoplasmic reticulum stress leading to reduced secretion.^[117] These assays, including pulse-chase labeling and electron microscopy, demonstrate how variants disrupt fibril assembly, providing evidence for pathogenicity under ACMG criteria.^[118] Such in vitro models are particularly informative for novel mutations, bridging genetic findings with phenotypic severity.^[117]

Nutritional Supplements and Therapies

Undenatured type II collagen (UC-II), derived from chicken sternum cartilage, is commonly administered as a nutritional supplement at a dosage of 40 mg per day for joint health support, particularly in osteoarthritis (OA).^[119] Meta-analyses of clinical trials indicate that UC-II supplementation leads to significant pain reduction in knee OA patients, achieving improvements of 20-30% or more on validated scales such as the Visual Analog Scale (VAS) and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) compared to placebo, often meeting or exceeding the minimal clinically important difference threshold.^[120] These benefits are attributed to UC-II's ability to modulate immune responses and reduce joint inflammation without altering the cartilage's structural integrity. Hydrolyzed forms of type II collagen, consisting of bioactive peptides with molecular weights typically between 1-10 kDa, offer enhanced bioavailability due to improved intestinal absorption, with peptides detectable in blood within one hour of ingestion and accumulating in joint tissues.^[121] These peptides promote chondrocyte activity and extracellular matrix synthesis, supporting joint repair in OA. When combined with glucosamine, hydrolyzed type II collagen has demonstrated reduced cartilage degradation and improved function in clinical studies, such as those involving hand and knee OA patients over 6-12 months.^[121] Oral tolerance therapy using type II collagen aims to induce immune tolerance by promoting regulatory T cells (Treg cells) that suppress autoimmune responses against joint cartilage. A 1996 double-blind, placebo-controlled pilot study by Sieper et al. involving early rheumatoid arthritis (RA) patients found no overall significant differences between daily oral doses of 1-10 mg bovine type II collagen for 12 weeks and placebo, though a non-significant trend toward reduced swollen and tender joint counts was observed in early RA patients at 10 mg/day.^[122] Injectable therapies incorporating type II collagen scaffolds facilitate cartilage regeneration by providing a biocompatible matrix for autologous chondrocyte implantation. The matrix-induced autologous chondrocyte implantation (MACI) procedure, for instance, seeds patient-derived chondrocytes onto a collagen-based scaffold to repair full-thickness knee cartilage defects up to 4 cm², leading to hyaline-like tissue formation and superior pain relief and function at 2-5 years post-implantation versus microfracture, as evidenced by the SUMMIT trial.^[123] Broader applications of type II collagen scaffolds in regenerative medicine enhance subchondral bone vascularization and cartilage integration in preclinical models.^[124] Type II collagen supplements, including both undenatured and hydrolyzed forms, hold Generally Recognized as Safe (GRAS) status from the FDA for human consumption at recommended doses.^[125] They are generally well-tolerated, with rare gastrointestinal side effects such as mild diarrhea or stomach discomfort reported in less than 5% of users across clinical trials. However, long-term safety data are limited to studies of up to 24 months, showing no serious adverse events or changes in organ function.^[119]

Emerging Research Directions

Recent advances in gene editing have focused on CRISPR-based technologies to model and potentially correct mutations in the COL2A1 gene, which encodes Type II collagen. Base editing techniques using CRISPR/Cas9 have been employed to introduce heterozygous mutations, such as p.R719C, into induced pluripotent stem cells (iPSCs) derived from healthy donors, creating accurate in vitro models of chondrodysplasias like precocious osteoarthritis.^[126] These iPSC lines enable the study of disease mechanisms by differentiating into chondrocytes that recapitulate pathological fibril assembly defects.^[127] Similarly, editing to introduce the p.G1170S mutation in iPSCs has provided insights into skeletal dysplasia progression, highlighting disrupted endochondral ossification.^[128] Preclinical efforts are advancing toward therapeutic applications, with CRISPR/Cas9 gene editing explored to correct COL2A1 mutations in cellular models of collagen disorders, though challenges like off-target effects persist.^[129] These approaches underscore the potential for personalized therapies in collagenopathies, with ongoing studies optimizing delivery for clinical translation.^[130] In biomaterial engineering, 3D-printed scaffolds incorporating type II collagen are emerging for cartilage and enthesis repair, with preclinical studies showing improved tissue integration. Integration with hydrogels, such as hyaluronic acid-collagen composites, further advances this field by enabling injectable, 3D-printable formulations that fill cartilage defects and sustain Type II collagen release for prolonged tissue remodeling.^[131] These hybrid materials demonstrate superior biocompatibility and load-bearing capacity in preclinical evaluations, paving the way for minimally invasive cartilage reconstruction.^[39] Epigenetic therapies targeting Type II collagen dysregulation in osteoarthritis (OA) are gaining traction, particularly through histone deacetylase (HDAC) inhibitors that modulate chromatin accessibility to restore gene expression. Selective HDAC inhibitors, such as those targeting class IIa enzymes, have been shown to attenuate inflammatory responses in chondrocytes, upregulating Type II collagen synthesis while suppressing catabolic factors like MMP-13 in OA models.^[132] These compounds counteract interleukin-1β-induced repression of chondrogenic markers, promoting matrix homeostasis in human cartilage explants.^[133] Complementing this, miRNA mimics are being explored to enhance chondrogenesis by fine-tuning regulatory networks; for instance, miR-27b mimics directly promote Type II collagen expression by targeting inhibitory pathways in mesenchymal stem cells.^[134] Similarly, miR-218 mimics accelerate early chondrogenic differentiation, increasing Type II collagen deposition in scaffold-based cultures.^[135] These therapies hold potential for OA treatment by addressing epigenetic silencing without genetic alteration. Links between the gut microbiome and Type II collagen-related immunity are under investigation, with gut dysbiosis implicated in impairing oral tolerance mechanisms. Dysbiotic microbiota alterations, observed in collagen-induced arthritis models, disrupt immune homeostasis and exacerbate joint inflammation by reducing regulatory T-cell responses to oral Type II collagen antigens.^[136] Studies from 2023 demonstrate that undenatured Type II collagen (UC-II) supplementation induces oral tolerance, mitigating arthritis severity through microbiome modulation and enhanced anti-inflammatory cytokine production.^[137] Probiotics, particularly Lactobacillus casei, synergize with UC-II to amplify these effects, restoring microbial balance and boosting suppression of inflammatory pathways in OA preclinical models.^[138] This interplay suggests microbiome-targeted interventions could enhance UC-II efficacy in autoimmune joint disorders. Artificial intelligence (AI) is revolutionizing the prediction of Type II collagen mutation impacts, with computational models simulating fibril mechanics to forecast disease outcomes. Deep learning frameworks, trained on molecular dynamics data, predict how COL2A1 mutations alter triple-helix stability and fibril assembly, correlating structural disruptions with clinical severity in chondrodysplasias.^[139] These AI-driven simulations enable rapid screening of variants, revealing mechanics-informed lethality risks analogous to those in related collagens.^[140] In 2024 publications, deep learning applications extended to epitope mapping have improved identification of immunogenic sites on Type II collagen, aiding vaccine design for autoimmune conditions by predicting MHC binding affinities with high precision.^[141] Such tools accelerate therapeutic discovery by integrating epitope predictions with fibril mechanics, prioritizing high-impact mutations for experimental validation.^[142]

References

[1]
The Collagen Family - PMC - PubMed Central
Four collagens are type II membrane proteins that also exist in a soluble form released from the cell surface by shedding. Collagens play structural roles and ...
[2]
In Situ D-periodic Molecular Structure of Type II Collagen - PMC
Type II collagen fibrils are major components of cartilage, intervertebral discs, and the vitreous humor of the eye and are vital to the normal development of ...
[3]
Collagen type II: From biosynthesis to advanced biomaterials for ...
Collagen type II exhibits a triple stranded, coiled rod-like structure, is expressed as a homotrimer (i.e. [a1(II)]3) and is synthesised exclusively by ...Fig. 1 · 4. Collagen Type Ii... · Table 2
[4]
Type II Collagen Disorders Overview - GeneReviews - NCBI - NIH
Apr 25, 2019 · Kniest dysplasia, COL2A1-related, is a very severe type II collagen disorder, but results in live birth and longer survival. The clinical ...Clinical Characteristics of Type... · Differential Diagnosis of Type... · Management
[5]
Entry - *120140 - COLLAGEN, TYPE II, ALPHA-1; COL2A1 - OMIM
Other Disorders Caused by COL2A1 Mutations. Other disorders caused by mutation in the COL2A1 gene include spondylometaphyseal dysplasia (SMD; 184252; see ...
[6]
Type II Collagen Gene Variants and Inherited Osteonecrosis of the ...
Jun 2, 2005 · Furthermore, mutation of the COL2A1 gene is known to be the cause of congenital disorders of the skeletal system. The results of the current ...<|control11|><|separator|>
[7]
COL2A1 collagen type II alpha 1 chain [ (human)] - NCBI
Sep 27, 2025 · Mutation in COL2A1 gene is associated with Retinal detachment and infantile-onset glaucoma in Stickler syndrome. In summary, it is not easy to ...
[8]
Identification and functional characteristics of a novel splicing ...
Jul 9, 2024 · The COL2A1 gene maps on to 12q13.11-q13.2, contains 54 exons, and encodes the type II collagen precursor protein (Nishimura et al., 2005).<|control11|><|separator|>
[9]
COL2A1 - Collagen alpha-1(II) chain - Homo sapiens (Human)
Jan 23, 2007 · Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth.
[10]
Mouse type II collagen gene. Complete nucleotide sequence, exon ...
The overall identity of the coding sequences of the mouse and human type II collagen genes is 89% at the nucleotide level, but only 37 amino acid changes occur ...
[11]
The triple helix of collagens – an ancient protein structure that ... - NIH
The triple helix is a protein structure of fundamental importance in building the extracellular matrix that enabled animal multicellularity and tissue ...Missing: homotrimer composition
[12]
COLLAGEN STRUCTURE AND STABILITY - PMC - NIH
The hydroxylation of Pro residues in the Yaa position of collagen increases dramatically the thermal stability of triple helices (Table 2). This ...
[13]
Collagen triple-helix formation in all-trans chains proceeds ... - PNAS
The results show that triple-helix formation occurs with a rate constant of 113 ± 20 s –1 at 3.7°C and is virtually independent of temperature, indicating a ...
[14]
The quest for substrates and binding partners: A critical barrier for ...
Sep 1, 2020 · ADAMTS2 seems to be the physiologically relevant procollagen N-propeptidase and can remove the propeptide of the fibrillar collagen types I, II, ...
[15]
Collagen fibril assembly: New approaches to unanswered questions
Here, we review current models of collagen fibril self-assembly, and how cells might exert control over the self-assembly process to define the number, length ...
[16]
Molecular properties and fibril ultrastructure of types II and XI ... - NIH
It is known that cartilage typically has two distinct populations of collagen fibrils; one thin (~20 nm in diameter) and the other thick (>40 nm diameter) (Hagg ...
[17]
Temporal Changes in Collagen Cross-Links in Spontaneous ...
Apr 12, 2012 · Cross-link type is predominantly determined by the extent of hydroxylation of specific lysine residues catalyzed by lysyl hydroxylases (LHs), ...Rabbit Osteochondral Defect... · Collagen And Hyl Content · Collagen Cross-Link Analysis<|control11|><|separator|>
[18]
SOX9 directly regulates the type-ll collagen gene | Nature Genetics
Jun 1, 1997 · These results demonstrate that COL2A1 expression is directly regulated by SOX9 protein in vivo and implicate abnormal regulation of COL2A1 ...
[19]
A Novel Regulatory Mechanism of Type II Collagen Expression via a ...
The SOX9/5/6 combination enhances Col2a1 transcription through a novel enhancer in intron 6 together with the enhancer in intron 1.
[20]
transcription factors SOX9 and SOX5/SOX6 cooperate genome-wide ...
SOX9 is a transcriptional activator required for chondrogenesis, and SOX5 and SOX6 are closely related DNA-binding proteins that critically enhance its ...
[21]
Alternative Splicing of Type II Procollagen: IIB or not IIB? - PMC - NIH
Over two decades ago, two isoforms of the type II procollagen gene (COL2A1) were discovered. These isoforms, named IIA and IIB, are generated in a ...
[22]
Alternative Splicing of Type II Procollagen Exon 2 Is Regulated by ...
Alternative splicing of the type II procollagen gene (COL2A1) is developmentally regulated during chondrogenesis. Chondropro- genitor cells produce the type ...
[23]
Biochemistry, Collagen Synthesis - StatPearls - NCBI Bookshelf - NIH
Transcription of mRNA in the nucleus. Genes for pro-a1 and pro-a2 chains are transcribed · Translation. mRNA moves into the cytoplasm and interacts with ...
[24]
Roles of the endoplasmic reticulum–resident, collagen-specific ... - NIH
Heat shock protein 47 (Hsp47) is an endoplasmic reticulum (ER)-resident molecular chaperone essential for correct folding of procollagen in mammalian cells.
[25]
Parallel expression of Sox9 and Col2a1 in cells ... - PubMed - NIH
However, no Sox9 expression was detected in hypertrophic chondrocytes; only low levels of Col2a1 RNA were found in the upper hypertrophic zone. Coexpression ...
[26]
Significance of Type II Collagen Posttranslational Modifications
Jun 8, 2023 · The aim of this review is to summarize the recent knowledge on COL2 posttranslational modifications with relevance to RA pathophysiology, diagnosis and ...
[27]
Posttranslational modifications of collagens as targets of hypoxia ...
In this brief review, we have highlighted the role of hypoxia and Hif-1α in regulating posttranslational modifications of collagens.
[28]
Glycosylation Modulates the Structure and Functions of Collagen
Thus, the elongation from the collagen triple helix (<2 nm diameter, 300 ... triple helix with pitch variation and two proline ring conformations. Food ...
[29]
Lysine post-translational modifications of collagen - PMC
Lysine modifications of collagen are highly complicated sequential processes catalysed by several groups of enzymes leading to the final step of biosynthesis.
[30]
Type II collagen defects in the chondrodysplasias. I ... - PubMed
Type II collagen defects in the chondrodysplasias. I ... Amino acid analysis indicated that the abnormal collagens have a higher ratio of hydroxylysine ...
[31]
A selective ER‐phagy exerts procollagen quality control via a ...
Dec 17, 2018 · Autophagy is a cytosolic quality control process that recognizes substrates through receptor‐mediated mechanisms.
[32]
Procollagen trafficking, processing and fibrillogenesis
Apr 1, 2005 · Here, we focus on the synthesis, trafficking, post-translational processing and assembly of collagen into fibrils, as well as some potentially important ...
[33]
Mini reviews The procollagen N-proteinases ADAMTS2, 3 and 14 in ...
ADAMTS2, 3 and 14 cleave the amino-propeptide of fibrillar collagens. ADAMTS2 deficiency leads to the dermatosparactic type of Ehlers–Danlos syndrome.Missing: BMP- | Show results with:BMP-
[34]
pH effects on collagen fibrillogenesis in vitro - ScienceDirect.com
Jun 1, 2009 · It has been found that the conditions for the formation of native type fibrils are in the pH range of 5.0–8.5, ionic strength between 0.1 and ...
[35]
Cartilage oligomeric matrix protein and its binding partners in the ...
In its pentameric state, COMP is also known to promote fibrillogenesis of type I and type II collagens. COMP interacts mainly with free type I and type II ...
[36]
Collagen fibrillogenesis: fibronectin, integrins, and ... - PubMed Central
This review puts forward a working hypothesis for how fibronectin and integrins (the organizers) determine the site of fibril assembly, and collagens V and XI ...<|control11|><|separator|>
[37]
Self-assembly into fibrils of collagen II by enzymic ... - PubMed
Apr 15, 1994 · The lag time for assembly of collagen II fibrils was 5-6-fold longer, and the propagation rate for collagen II fibrils was about 30-fold lower than for ...
[38]
Type II collagen – Knowledge and References - Taylor & Francis
Type II collagen is mainly produced by chondrocytes and composed of 50–80% the dry weight of articular cartilage. It is insoluble and exhibits the mechanical ...
[39]
Type II collagen scaffolds for tissue engineering - Nature
Aug 10, 2024 · The biosynthesis of CII is a multi-step process that commences with the transcription of the COL2A1 gene, encoding α1(II) polypeptide chains ( ...
[40]
The Basic Science of Articular Cartilage - NIH
The interaction between the highly negatively charged cartilage proteoglycans and type II collagen provides the compressive and tensile strength of the tissue.
[41]
Articular Cartilage: A Brief Review of its Structure, Function, and ...
Aug 19, 2016 · The type II collagen fibrils are thinner near the articular surface and the tangential zone than that are in the deeper zones, and the collagen ...Missing: entrapment | Show results with:entrapment
[42]
Articular cartilage and changes in Arthritis - PubMed Central - NIH
After skeletal growth has ceased, the synthetic rate of type II collagen by articular chondrocytes drops dramatically as assessed by proline labeling in vivo.Missing: secretion | Show results with:secretion
[43]
Collagen type II: From biosynthesis to advanced biomaterials for ...
Collagen type II is the major constituent of cartilage tissue, and is advocated as a building block in cartilage engineering.Collagen Type Ii: From... · 2. Cartilage Development... · 4. Collagen Type Ii...
[44]
Articular Cartilage - Basic Science - Orthobullets
type II collagen accounting for 90% to 95% of the total collagen content. zones based on the shape of the chondrocytes and the orientation of the type II ...
[45]
Depth-Dependent Strain Model (1D) for Anisotropic Fibrils in ... - MDPI
Jan 1, 2024 · Most of the collagen in articular cartilage is type II, which is fibril collagen. ... For the SZ the modulus is ~1 MPa and it increases to ...1. Introduction · 2. Materials And Methods · 3. ResultsMissing: Young's | Show results with:Young's
[46]
Effect of collagen turnover on the accumulation of advanced ...
Dec 15, 2000 · From the age-related increases in % d-Asp the half-life of cartilage collagen was calculated to be 117 years and that of skin collagen 15 years ...
[47]
Role of Chondrocytes in Cartilage Formation, Progression of ... - NIH
Dec 18, 2015 · Chondrocytes recognize the loss of ECM and actively produce collagen type II and proteoglycans. However, the ratio between the ECM protein ...
[48]
Collagen Type II | Harvard Catalyst Profiles
A fibrillar collagen found predominantly in CARTILAGE and vitreous humor. It consists of three identical alpha1(II) chains. Descriptor ID. D024043. MeSH ...
[49]
Adult vitreous structure and postnatal changes | Eye - Nature
Feb 29, 2008 · Type VI collagen has been shown to bind fibrillar collagens and HA, so it could have a role in linking together the heterotypic collagen fibrils ...
[50]
Enzymatic Breakdown of Type II Collagen in the Human Vitreous
Type II collagen is predominant and consists of approximately 80% of total vitreous collagen. In vitro studies in ox vitreous 12 and in vivo studies in chick ...
[51]
The Vitreous Glycoprotein Opticin Inhibits Preretinal ...
Opticin is a glycoprotein that we discovered associated with vitreous humor collagen fibrils. Subsequently, it has been identified in other tissues including ...
[52]
Intervertebral Disc Collagen - Journal of Biological Chemistry
Nucleus pulposus, the central zone of the intervertebral disc, is gel-like and has a similar collagen phenotype to that of hyaline cartilage.
[53]
Intervertebral Disk Degeneration: The Microenvironment and Tissue ...
Jul 19, 2021 · Type II collagen is the most critical collagen in the NP for forming irregular networks to support PGs and water. The distinctive arrangement ...
[54]
Co-expression of collagens II and XI and alternative splicing of exon ...
Low levels of procollagen alpha 2(XI) mRNA were also seen in non-cartilaginous tissues: calvarial and diaphyseal bone, kidney, skin, muscle, intestine, liver, ...
[55]
Tectorins crosslink type II collagen fibrils and connect the tectorial ...
The tectorial membrane (TM) is a gelatinous acellular membrane located above the hearing sensory epithelium and is composed mostly of type II collagen, and α– ...
[56]
Collagen--the predominant protein of the tectorial membrane
Evidence is presented that, in contrast to the traditional view, a large proportion of the proteins of the tectorial membrane (TM) consists of collagen, ...
[57]
Collagen II Is Essential for the Removal of the Notochord and the ...
Collagen II is a fibril-forming collagen that is mainly expressed in cartilage. Collagen II–deficient mice produce structurally abnormal cartilage that ...
[58]
Expression of the mouse α1 (II) collagen gene is not restricted to ...
Apr 1, 1991 · In the trunk region, type II collagen is found abundantly in the notochord and the sclerotomal mesenchyme. The appearance of type II collagen ...
[59]
Localization of type I and II collagen during development of human ...
In childhood and early adolescence type I collagen was found to be restricted to the perichondrium of first rib cartilage, while type II collagen was localized ...
[60]
Molecular Adhesion between Cartilage Extracellular Matrix ... - NIH
Aggrecan–collagen adhesion thus mainly takes place between aggrecan and type II collagen ... electrostatic interactions govern aggrecan–collagen adhesion in vivo.
[61]
Molecular Adhesion between Cartilage Extracellular Matrix ...
Although previous studies have suggested high binding affinity of KS-GAGs to type II collagen, (9) our experiment did not investigate the KS-GAG and collagen ...
[62]
Association of the Aggrecan Keratan Sulfate-rich Region with ...
These observations support the view that the interaction depends on the core protein. Figure viewer. Figure 3 Binding of aggrecan fragments to isolated collagen ...
[63]
Collagen XI regulates the acquisition of collagen fibril structure ...
Collagen XI is a fibril-forming collagen that regulates collagen fibrillogenesis. Collagen XI is normally associated with collagen II-containing tissues ...
[64]
Assembly of collagen types II, IX and XI into nascent hetero‐fibrils by ...
Jul 9, 2003 · The results indicate that the assembly and intertype cross-linking of the cartilage type II collagen heteropolymer is an integral, early process in fibril ...
[65]
Type IX collagen proteoglycan from cartilage is covalently cross ...
Feb 5, 1988 · The results show that type IX collagen is linked to type II collagen by a hydroxypyridinium cross-link. This cross-link connects the central ...
[66]
evidence of a differential role for α1β1 and α2β1 integrins in ...
Cell adhesion assays revealed that both α1β1 and α2β1 could serve as chondrocyte adhesion receptors for types II and VI collagen. In cell lines expressing both ...
[67]
Integrin expression by primary and immortalized human chondrocytes
Cell adhesion assays revealed that both α1β1 and α2β1 could serve as chondrocyte adhesion receptors for types II and VI collagen. In cell lines expressing both ...
[68]
Integrins and chondrocyte-matrix interactions in articular cartilage
These integrins bind a host of cartilage extracellular matrix (ECM) proteins, most notably fibronectin and collagen types II and VI, which provide signals that ...
[69]
Peptides of type II collagen can induce the cleavage of type II ...
The objective of this study was to determine whether a fragment(s) of type II collagen can induce cartilage degradation. Fragments generated by cyanogen ...
[70]
Cartilage extracellular matrix-derived matrikines in osteoarthritis
Type II collagen peptide stimulates Akt leading to nuclear factor-κB activation: its inhibition by hyaluronan. Biomed Res 35: 193–199, 2014. Crossref · PubMed.
[71]
Overview of MMP-13 as a Promising Target for the Treatment of ...
MMP-13 is the primary catabolic factor involved in cartilage degradation through its particular ability to cleave type II collagen. The breakdown products of ...
[72]
Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 ...
Various MMPs play important roles in collagen degradation. MMP-1, -8, -13 ... MMP-13 has higher affinity for type II collagen than MMP-1 or -8 but can ...
[73]
Increased type II collagen cleavage by cathepsin K and collagenase ...
May 14, 2012 · The intra-helical cleavage of type II collagen by proteases, including collagenases and cathepsin K, is increased with aging and ...Missing: matricryptins | Show results with:matricryptins<|separator|>
[74]
Expression of Two Novel Alternatively Spliced COL2A1 Isoforms ...
Alternative splicing of the type II procollagen gene (COL2A1) is developmentally-regulated during chondrogenesis. Type IIA procollagen (+ exon 2) is ...
[75]
A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed ...
A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene. · Abstract · Full Text.
[76]
Gene regulation during cartilage differentiation: temporal and spatial ...
Sep 1, 1989 · Spatially, type II collagen transcripts are the first to be detected in situ in all prechondrogenic regions during the stages of limb ...
[77]
The growth plate: a physiologic overview - PMC - NIH
The major structural collagen of the growth plate cartilage is type II collagen, a strong fibrous complex of three identical strands interwoven for strength.
[78]
Endochondral ossification: A delicate balance between growth and ...
For example, mice lacking type II collagen show increased chondrocyte apoptosis, suggesting that this collagen provides an attachment substrate and essential ...
[79]
Disrupted type II collagenolysis impairs angiogenesis, delays ...
Aug 2, 2019 · Disrupted type II collagenolysis impairs angiogenesis, delays endochondral ossification and initiates aberrant ossification in mouse limbs.Missing: helix hyperhydroxylation electrophoresis
[80]
Type XXVII collagen at the transition of cartilage to bone ... - NIH
Type II collagen remained throughout the structure. Type X collagen was prominent in the ECM surrounding hypertrophic cells. Type XXVII collagen was ...
[81]
The Contribution of Meckel's Cartilage-Derived Type II Collagen ...
Jun 5, 2024 · Our investigation has identified the presence of type II collagen positive (Col2 + ) cells within the jawbone development and regeneration.
[82]
Mandibular Condylar Cartilage in Development and Diseases
Apr 19, 2025 · Chondrocytes in the MCC undergo rapid hypertrophic differentiation and express both types II collagen (COLII) and type X collagen (COLX) in the ...
[83]
Bone morphogenetic protein (BMP)-2 enhances the expression of ...
Bone morphogenetic protein (BMP)-2 enhances the expression of type II collagen and aggrecan in chondrocytes embedded in alginate beads · Authors · Affiliation.
[84]
TGF-β and BMP signaling in osteoblast, skeletal development ... - NIH
This review summarizes the recent advances in our understanding of TGF-β/BMP signaling in osteoblast differentiation, chondrocyte differentiation, skeletal ...
[85]
Interaction of TGFβ and BMP Signaling Pathways during ...
In summary, our data demonstrate that during endochondral ossification, BMP and TGFβ signaling can have antagonistic effects on chondrocyte proliferation and ...
[86]
Mutation Update for COL2A1 Gene Variants Associated with Type II ...
Mutations in the COL2A1 gene cause a spectrum of rare autosomal-dominant conditions characterized by skeletal dysplasia, short stature, and sensorial defects.
[87]
Molecular genetics of the COL2A1-related disorders - ScienceDirect
Mutations in the collagen type II alpha-1 gene (COL2A1) have been reported to be responsible for a series of abnormalities, known as type II collagenopathies.
[88]
Enhanced cleavage of type II collagen by collagenases in ... - NIH
We demonstrate the direct involvement of increased collagenase activity in the cleavage of type II collagen in osteoarthritic human femoral condylar cartilage ...Missing: matricryptins | Show results with:matricryptins
[89]
https://europepmc.org/articles/pmc1219184
[90]
Overview of MMP-13 as a Promising Target for the Treatment ... - MDPI
MMP-13 (collagenase 3) is the key enzyme in the cleavage of type II collagen and plays a pivotal role in the breakdown of cartilage in osteoarthritic joints [17] ...
[91]
Developmental Mechanisms in Articular Cartilage Degradation in ...
Dec 29, 2010 · This is accompanied by severe proteoglycan loss followed by degradation of type II collagen [164, 180]. Collagen degradation occurs around ...
[92]
On how degeneration influences load-bearing in the cartilage–bone ...
This study investigated the microanatomical response to compression of intact and degenerate cartilage-on-bone samples with the aim of elucidating the ...<|control11|><|separator|>
[93]
Aging Cartilage and Osteoarthritis--What's the Link? - Science
Pentosidine formation, which can cross-link collagen molecules, might be an important factor in the increase in collagen stiffness and altered cartilage ...
[94]
Role of advanced glycation end products in mobility and ...
Oct 10, 2018 · One of the major age-related changes in articular cartilage is increased levels of cross-linked AGEs. From age 20 onward, AGEs accumulate in ...
[95]
Age-dependent changes in protein incorporation into collagen-rich ...
Sep 28, 2021 · We report dynamic turnover of the matrisome, the proteins of the extracellular matrix, in bone and cartilage during skeletal maturation, which ...
[96]
Can urinary CTX-II be a biomarker for knee osteoarthritis?
Feb 10, 2020 · According to systematic reviews, urinary CTX-II is the most effective cartilage degradation biomarker in most aspects in terms of diagnosis, ...
[97]
Obesity aggravates the joint inflammation in a collagen-induced ...
White fat cells secrete adipokines that induce inflammation and obesity has been reported to be characterized by high serum levels of inflammatory cytokines ...Missing: turnover fragments
[98]
Expression and regulation of Toll-like receptor 2 by IL-1β and ...
TLR2 was upregulated following IL-1β and fibronectin proteolytic fragments stimulation in primary cultures of osteoarthritic articular chondrocytes. Fibronectin ...Materials And Methods · Tlr2 Mrna Expression By... · Myd88 And Nf-κb Activation...
[99]
The cartilage degradation marker, urinary CTX-II, is associated with ...
It was found that baseline urinary CTX-II, a marker of cartilage type II collagen degradation, was one of the most prognostic biomarkers for disease progression ...<|control11|><|separator|>
[100]
Urinary CTX-II concentrations are elevated and associated with ...
This study examined the magnitude and change over time in urinary CTX-II (uCTX-II) concentrations shortly after ACL reconstruction, and, secondarily, the ...
[101]
Genetic control of tolerance to type II collagen and development of ...
T cell recognition of the type II collagen (CII) 260-270 peptide is a bottleneck for the development of collagen-induced arthritis (CIA), an animal model of ...
[102]
Type II collagen autoimmunity in rheumatoid arthritis - PubMed
This review summarizes the autoimmune reaction to type II collagen (CII) autoimmunity with regard not only to antibody response to CII but also to the ...
[103]
Antibodies to type II collagen in early rheumatoid arthritis ... - PubMed
The presence of antibodies to native collagen was associated with activity of RA and severity of symptoms, and loss of antibodies at 12 months was associated ...
[104]
Type II collagen oral tolerance; mechanism and role in ... - PubMed
Aug 21, 2009 · Repeated oral administrations of type II collagen (CII) induce oral tolerance and inhibit the development of collagen-induced arthritis (CIA).
[105]
Proinflammatory role of the Th17 cytokine interleukin-22 in collagen ...
Objective: To investigate the role of interleukin-22 (IL-22) in collagen-induced arthritis (CIA), an animal model of rheumatoid arthritis. Methods: C57BL/6 mice ...
[106]
Antibodies to type II collagen in relapsing polychondritis - PubMed
Antibodies to Type II (cartilage) collagen were found in the serum of five patients at the time of acute symptoms. No antibodies were detected.
[107]
pathogenic role of anti-native collagen type II antibodies ... - PubMed
Relapsing polychondritis - pathogenic role of anti-native collagen type II antibodies. A case report with immunological and pathological studies · Abstract.
[108]
Collagen-induced arthritis, an animal model of autoimmunity - PubMed
Immunization of genetically susceptible strains of rodents and primates with type II collagen (CII) leads to the development of a severe polyarticular arthritis
[109]
Incidence of serum antibodies to native type I and type II collagens ...
Raised serum IgG antibody levels to native type II collagen were present in 42% of patients with RA, 30% with AS, and none of the patients with psoriatic ...
[110]
Best practice guidelines regarding diagnosis and management of ...
Somatic mosaicism for COL2A1 variants is well recognized in the medical literature to occur in 6–10% of parents of children with apparent germline mutations in ...
[111]
https://www.jcrpe.org/articles/diagnostic-challenge-of-phenotypic-variability-in-lessemgreatercol2a1lessemgreater-related-disorders-four-novel-variants-that-expand-the-clinical-spectrum/jcrpe.galenos.2025.2024-9-7
[112]
Combined detection of serum CTX-II and COMP concentrations in ...
Nov 22, 2016 · Cartilage oligomeric matrix protein (COMP) and crosslinked C-telopeptides of type II collagen (CTX-II) are two biochemical markers; they are ...
[113]
C-Terminal Cross-Linked Telopeptides of Type II Collagen as ...
According to our findings, urinary CTX-II levels have a potential to distinguish knee OA patients from healthy controls which can serve as biomarker for knee OA ...
[114]
Biochemical markers of type II collagen breakdown and synthesis ...
Cartilage turnover assessed with a newly developed assay measuring collagen type II degradation products: influence of age, sex, menopause, hormone ...
[115]
Immunohistochemical study of collagen types I and II and ...
Type II collagen makes up more than 90% of the collagen found in adult articular (hyaline) cartilage [6] and it occurs to a smaller extent in fibrocartilage ...
[116]
Characteristics of Anti-Type II Collagen Antibody Binding to Articular ...
With normal human articular cartilage, the cut surfaces bound approximately 4 times as much anti-CII antibody as the intact articular surfaces. These findings ...Missing: biopsy | Show results with:biopsy
[117]
Characterization of a type II collagen gene (COL2A1) mutation ... - NIH
A subtle mutation in the type II collagen gene COL2A1 was detected in a case of human hypochondrogenesis by using a chondrocyte culture system and PCR-cDNA ...Missing: functional tests assays
[118]
Clinical and functional characterization of COL2A1 p.Gly444Ser ...
These results showed that p.Gly444Ser variant alters procollagen molecules processing and the assembly of mature type-II collagen fibrils.Missing: assays | Show results with:assays
[119]
Undenatured Type II Collagen (UC-II) in Joint Health and Disease
Many clinical and experimental studies have revealed that the undenatured form of type II collagen (UC-II) offers common health benefits to patients with OA.<|separator|>
[120]
[PDF] Review Effect of collagen supplementation on knee osteoarthritis
The results of this meta- analysis suggest that oral collagen administration relieves OA symptoms. Our findings revealed noteworthy im- provements, ...<|separator|>
[121]
Collagen Supplementation for Joint Health: The Link between ...
Mar 8, 2023 · Hydrolyzed collagen may contain biologically active peptides that are able to reach joint tissues and exert chondroprotective effects. Although ...
[122]
Oral type II collagen treatment in early rheumatoid arthritis. A double ...
Methods: Ninety patients with RA (disease duration < or = 3 years) were treated for 12 weeks with oral bovine type II collagen at 1 mg/day (n = 30) or 10 mg/day ...Missing: phase | Show results with:phase
[123]
Repair Knee Cartilage Defects Using a Patient's Own Cells - MACI
MACI is an autologous cellularized scaffold product indicated for the repair of single or multiple symptomatic, full-thickness cartilage defects of the knee.
[124]
Recent strategies of collagen-based biomaterials for cartilage repair
Mar 28, 2022 · Collagen type II (COL II) in articular cartilage accounts for approximately 90–95% of the extracellular matrix collagen. The characteristic ...
[125]
Bioiberica obtains self-determined GRAS status for Collavant n2 ...
Jul 2, 2024 · Collavant n2 native (undenatured) type II collagen (40mg/day) has obtained self-determined GRAS status, allowing brands to harness its joint health benefits.<|control11|><|separator|>
[126]
Using CRISPR/Cas9 to generate a heterozygous COL2A1 p.R719C ...
Aug 24, 2025 · To develop an in vitro disease model of a human chondrodysplasia, we used CRISPR/Cas9 gene editing to generate a heterozygous COL2A1 exon 50 c. ...
[127]
Using CRISPR/Cas9 to generate a heterozygous COL2A1 p.R719C ...
The human iPSC line MCRIi019-A-6 was generated using CRISPR/Cas9-mediated gene editing to introduce a heterozygous COL2A1 exon 33 c.2155 C>T (p.R719C) mutation ...
[128]
Modeling of skeletal development and diseases using human ...
, et al. CRISPR/Cas9 editing to generate a heterozygous COL2A1 p.G1170S human chondrodysplasia iPSC line, MCRIi019-a-2, in a control iPSC line, MCRIi019-a.
[129]
Gene editing for collagen disorders: current advances and future ...
Aug 11, 2025 · Recent preclinical studies have focused on CRISPR/Cas9-based methods to address the dominant-negative pathogenic variants in the genes ...
[130]
CRISPR/Cas9 and iPSC-Based Therapeutic Approaches in ... - MDPI
CRISPR/Cas9 and hiPSCs are promising for disease modeling and therapy, but off-target effects and the complexity of gene editing in the brain limit their use.Crispr/cas9 And Ipsc-Based... · 2. Crispr-Based Gene Editing... · 2.2. Gene-Editing Potential...
[131]
Promotion of bone-tendon healing after ACL reconstruction using ...
We aimed to enhance bone-tendon healing post-ACL reconstruction using scaffold-free cell constructs of ADSCs produced with a bio-3D printer. This allows the ...
[132]
[PDF] TOWARDS 3D BIOPRINTED ACL-ON-CH
Results showed improved osteointegration between tendon and tunnel bone in a rabbit model ACL reconstruction as assessed by haematoxylin and eosin staining,.
[133]
An injectable and 3D printable pro-chondrogenic hyaluronic acid ...
Oct 27, 2023 · An injectable and 3D printable pro-chondrogenic hyaluronic acid and collagen type II composite hydrogel for the repair of articular cartilage defects.Abstract · Introduction · Results · Discussion
[134]
A novel inhibitor of class IIa histone deacetylases attenuates ...
Sep 2, 2024 · A novel inhibitor of class IIa HDACs has been shown to have an anti-inflammatory and anti-arthritic profile distinct from current therapies. It ...
[135]
Histone deacetylase inhibitors attenuated interleukin-1β-induced ...
Jan 27, 2022 · This study aimed to confirm that histone deacetylase (HDAC) inhibitors, which alleviate arthritis, can improve chondrogenesis inhibited by IL-1β ...
[136]
miR-27b promotes type II collagen expression by targetting ...
siRNA-mediated knockdown of Pparγ2 caused an increase in protein levels of Col2a1. The present study demonstrates that miR-27b regulates chondrocyte hypertrophy ...<|control11|><|separator|>
[137]
MicroRNA-218 promotes early chondrogenesis of mesenchymal ...
Jan 15, 2019 · The transfection of miR-218 mimics notably enhanced SDSCs chondrocytes differentiation, as evidenced by augmented expressions of chondrogenic ...
[138]
“Cross-talk” between gut microbiome dysbiosis and osteoarthritis ...
Apr 24, 2023 · Available evidence indicated that GMB dysbiosis aggravated OA through activating the immune response and subsequent induction of inflammation.
[139]
The effect of oral administration of undenatured type II collagen on ...
Apr 20, 2023 · All doses of undenatured collagen supplementation may ameliorate MIA-induced OA symptoms, the higher doses showed faster improvement in gait measurements.
[140]
Clinical efficacy of probiotic supplementation in the treatment ... - NIH
Apr 9, 2025 · Lactobacillus casei enhances type II collagen/glucosamine-mediated suppression of inflammatory responses in experimental osteoarthritis.Missing: UC- | Show results with:UC-
[141]
Discovering design principles of collagen molecular stability using a ...
Sep 26, 2022 · We develop a deep learning framework to generate collagen sequences with desired thermal stability and validate our deep learning framework using both ...Missing: nucleation | Show results with:nucleation
[142]
Lethality prediction of osteogenesis imperfecta on type I collagen by ...
We propose a method to predict the risk of lethality for OI-inducing mutations, where a novel mechanics-informed graph representation of the collagen fibril
[143]
Integrating machine learning to advance epitope mapping - Frontiers
Sep 29, 2024 · There exist several accessible online machine learning tools for predicting T-cell epitopes and/or MHC class I and class II peptide binding ( ...Missing: collagen | Show results with:collagen
[144]
Integrating machine learning to advance epitope mapping - PMC - NIH
Sep 30, 2024 · In T-cell epitope prediction, we encounter the dichotomy of algorithms focused primarily on either MHC class I or MHC class II binding ...Missing: collagen | Show results with:collagen