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RUNX2

RUNX2, or runt-related transcription factor 2, is a protein-coding gene that encodes a key transcription factor in the RUNX family, essential for osteoblast differentiation, chondrocyte maturation, and overall skeletal development in vertebrates.^[1] It functions by binding to specific DNA consensus sequences as a heterodimer with core-binding factor beta (CBFB), thereby regulating the expression of genes involved in bone formation, proliferation of skeletal progenitor cells, and vascular invasion into developing bone tissues.^[1] Expressed primarily in multipotent mesenchymal cells, preosteoblasts, osteoblasts, and hypertrophic chondrocytes, RUNX2 orchestrates the transition from cartilage to bone during endochondral ossification and supports membranous ossification in flat bones.^[2] Its activity is tightly controlled by multiple signaling pathways, including hedgehog, fibroblast growth factor (FGF), Wnt, and parathyroid hormone-related protein (PTHLH), which collectively ensure precise spatiotemporal regulation of skeletal growth.^[1] In osteoblast lineage cells, RUNX2 promotes proliferation through upregulation of receptors like FGFR2 and FGFR3, and induces differentiation by activating downstream targets such as Sp7 (osterix) and genes encoding bone matrix proteins including Col1a1, Col1a2, Spp1 (osteopontin), Ibsp (bone sialoprotein), and Bglap (osteocalcin).^[2] It also facilitates the commitment of mesenchymal progenitors to the osteoblast lineage by integrating signals from Dlx5 and Wnt pathways.^[1] In chondrocytes, RUNX2 drives the maturation process from prehypertrophic to terminal hypertrophic stages, regulating proliferation via Indian hedgehog (Ihh) and inhibiting apoptosis to allow for transdifferentiation into osteoblasts, particularly in embryonic trabecular bone formation.^[2] Additionally, RUNX2 supports angiogenesis by inducing vascular endothelial growth factor A (Vegfa), enabling blood vessel invasion into mineralized cartilage templates.^[2] RUNX2 expression and activity are modulated post-translationally by enzymes such as protein kinase C (PKC) and extracellular signal-regulated kinase (ERK) through phosphorylation, peptidyl-prolyl isomerase PIN1 via isomerization, and histone deacetylases (HDACs) influencing acetylation, which collectively affect its stability, DNA binding, and transcriptional potency.^[3] Dysregulation of RUNX2 contributes to several bone-related disorders; heterozygous mutations lead to cleidocranial dysplasia (CCD), characterized by delayed cranial suture closure, hypoplastic or absent clavicles, and dental abnormalities due to haploinsufficiency.^[3] Overexpression or hyperactivation is implicated in craniosynostosis (premature suture fusion), may accelerate osteoarthritis progression by altering cartilage homeostasis, and the development of osteosarcoma and other cancers.^[3] ^[4] As a master regulator, RUNX2 represents a promising therapeutic target for conditions like osteoporosis, with potential interventions including HDAC inhibitors to enhance its activity in CCD or PIN1 inhibitors to mitigate excessive function in craniosynostosis.^[3] As of 2025, recent studies have highlighted RUNX2's roles in bone mechanotransduction via polycystins, hypomethylation as a potential biomarker for osteoporosis, and in the pathogenesis of osteosarcopenia.^[5]^[6]^[7]

Gene and Protein Overview

Genomic Structure and Location

The RUNX2 gene is located on the short arm of human chromosome 6 at band p21.1, spanning genomic coordinates 45,328,157 to 45,664,349 in the GRCh38 assembly, which corresponds to approximately 336 kb.^[8] In the mouse, the orthologous Runx2 gene resides on chromosome 17, from positions 44,806,874 to 45,125,684, encompassing about 319 kb.^[9] The gene's official identifiers include OMIM 600211, HGNC 10472, and Ensembl ENSG00000124813.^[10]^[11] The genomic architecture of RUNX2 features a complex structure with multiple exons due to alternative splicing, though principal transcripts are organized into 8–9 exons spanning the locus.^[10] Exon 1 serves as a non-coding region in certain variants, while exons 3 through 5 encode the core Runt homology domain essential for DNA binding.^[12] This organization includes two promoter regions associated with exons 1 and 2, contributing to regulatory diversity.^[10] The Runt homology domain exhibits high evolutionary conservation across vertebrates, reflecting its fundamental role in transcriptional regulation.^[13] Orthologs of RUNX2 are present in distant species, including the runt protein in Drosophila melanogaster and runx2a/runx2b in zebrafish, underscoring the ancient origins of the RUNX family.^[13]^[14]

Isoforms and Expression Patterns

RUNX2 produces three main isoforms through alternative promoter usage and splicing, designated as Type I, Type II, and Type III. The Type II isoform, also known as Runx2-II, is transcribed from the distal P1 promoter and initiates with the amino acid sequence MASNS; it is mesenchymal-specific and includes a glutamine-alanine (QA) domain at the N-terminus that modulates its transcriptional activity.^[15]^[16] In contrast, the Type I isoform, or Runx2-I, arises from the proximal P2 promoter, starts with MRIPV, lacks the QA domain, and predominates in committed osteoblasts.^[17]^[18] The Type III isoform represents a rare alternative splice variant, primarily identified in rodents such as mice and rats, sharing the same reading frame as Type II but with a distinct first exon; it is not observed in humans.^[16]^[19] Promoter usage dictates isoform specificity and tissue context. The P1 promoter is active in non-osseous mesenchymal tissues, driving Type II expression during early developmental stages to support broad progenitor commitment.^[20]^[21] Conversely, the P2 promoter activates in committed osteo- and chondroprogenitors, preferentially producing the Type I isoform to fine-tune differentiation in skeletal lineages.^[22]^[23] This differential promoter activity allows RUNX2 to adapt its isoform profile to varying cellular demands across development. RUNX2 exhibits dynamic spatiotemporal expression patterns, with high levels during embryonic skeletal development. In mice, Runx2 mRNA is first detectable at embryonic day 11.5 (E11.5) in limb buds, mandibular processes, and mesenchymal condensations destined for bone formation.^[22] Expression intensifies in differentiating osteoblasts, prehypertrophic and hypertrophic chondrocytes, and odontoblasts within the developing skeleton, peaking around E13.5 to E17.5 to orchestrate endochondral and intramembranous ossification.^[22]^[17] In adult tissues, RUNX2 levels are generally low in mature bone but become upregulated in response to injury, such as during fracture healing or osteoarthritis, where it supports repair by reactivating osteoblast and chondrocyte programs.^[24] Beyond skeletal contexts, RUNX2 is expressed in hair follicle stem cells, brain tissues, and various cancer cells, including those in osteosarcoma, breast cancer metastases, and oral squamous cell carcinoma, often correlating with aberrant proliferation or invasion.^[25]^[26]^[27]

Molecular Structure and Mechanism

Protein Domains and Architecture

The RUNX2 protein exhibits a modular architecture characteristic of the RUNT family of transcription factors, with the predominant Type II isoform comprising 521 amino acids in humans. This isoform spans all eight exons and includes distinct N-terminal, central, and C-terminal regions that facilitate nuclear localization, DNA interaction, and regulatory functions. The protein is predominantly nuclear due to multiple nuclear localization signals (NLS) positioned at both the N- and C-termini, ensuring efficient compartmentalization within the nucleus. Additionally, a nuclear matrix targeting signal (NMTS) in the C-terminal region directs RUNX2 to subnuclear foci associated with transcriptional activity.^[28]^[10] Central to the protein's function is the Runt homology domain (RHD), a conserved 128-amino-acid motif spanning residues 50–177, which mediates both sequence-specific DNA binding and heterodimerization with the non-DNA-binding partner CBFβ to enhance stability and affinity. N-terminal to the RHD lies the glutamine/alanine-rich (QA) domain, consisting of 23 glutamine and 17 alanine repeats, which contributes to transactivation potential. The transactivation domain (TAD) is multifaceted, with subdomains including an N-terminal activation domain 1 (AD1, first 19 residues) and the QA region acting as AD2; a C-terminal TAD (approximately residues 391–419) recruits co-activators to modulate gene expression. An inhibitory domain (ID, residues 338–376) within the proline/serine/threonine-rich (PST) region provides autoregulatory control by repressing excessive transcriptional activity, while the C-terminal VWRPY motif further contributes to inhibition.^[29]^[30]^[28] Structural features also include multiple phosphorylation sites that influence stability and activity, such as serine 347 and threonine 340 in the PST region (targeted by protein kinase A), serine 203, threonine 205, and threonine 207 in the RHD (by Akt), and serine 451 (by CDK1/cdc2). These modifications occur primarily on serine/threonine residues and are critical for fine-tuning protein function without altering the core architecture. The NLS sequences, including a 9-amino-acid motif (PRRHRQKLD) at the RHD-PST junction (approximately residues 204–220) and additional signals at the termini, ensure nuclear import. The NMTS, a 38-amino-acid sequence within the C-terminal PST domain (residues ~327–521), anchors RUNX2 to the nuclear matrix for localized gene regulation.^[28]^[31]^[10] Isoform variations arise from alternative promoter usage, with Type I (513 amino acids, initiating at exon 2 with MRIPVD) lacking the N-terminal exon 1-encoded QA domain present in Type II (starting with MASNSL), resulting in reduced transactivation capacity and protein stability for Type I. This architectural difference influences isoform-specific roles, though both retain the core RHD and C-terminal features. Type I is more broadly expressed, including in non-osseous tissues, while Type II predominates in osteoblasts.^[28]^[30]

DNA Binding and Transcriptional Activity

RUNX2 binds to DNA through its Runt homology domain (RHD), which specifically recognizes the consensus sequence 5'-PuACCPuCA-3' (where Pu represents a purine) within the promoter regions of target genes.^[32] This binding is stabilized by heterodimerization with the non-DNA-binding partner CBFβ, which enhances the affinity of RUNX2 for DNA by approximately 10-fold without directly contacting the DNA.^[33] The interaction with CBFβ induces a conformational change in the RHD, promoting efficient sequence-specific recognition and preventing proteasomal degradation of RUNX2.^[34] As a transcription factor, RUNX2 functions primarily as an activator of osteogenic genes, including Alpl (encoding alkaline phosphatase, ALP) and Bglap (osteocalcin), by recruiting RNA polymerase II and co-activators to enhancer elements.^[35] It also exhibits repressive activity on select targets through recruitment of histone deacetylases (HDACs), such as HDAC3, leading to chromatin condensation and transcriptional silencing.^[36] RUNX2's dual role allows it to oscillate between activation and repression in a context-dependent manner, influenced by cellular signals and co-factor availability.^[37] RUNX2's transcriptional mechanism involves cooperative interactions with other transcription factors, such as AP-1 family members, which bind adjacent sites to synergistically enhance promoter occupancy and gene activation.^[38] In osteoblasts, ChIP-seq analyses have identified thousands of RUNX2 binding sites associated with the direct regulation of numerous target genes, including Col1a1 (collagen type I alpha 1) and Bglap, underscoring its role as a master regulator of osteoblast-specific transcription.^[39]

Biological Functions

Role in Osteoblast Differentiation

RUNX2 is essential for the commitment of mesenchymal stem cells (MSCs) to the osteoblast lineage, directing their differentiation into pre-osteoblasts. In Runx2-null mice, this commitment is severely impaired, resulting in a complete lack of bone formation due to the maturational arrest of osteoblasts at an immature stage.^[40] Homozygous mutants exhibit no intramembranous or endochondral ossification, with skeletal elements remaining as cartilaginous templates, underscoring RUNX2's indispensable role in initiating osteoblast specification from multipotent progenitors.^[41] During osteoblast maturation, RUNX2 upregulates early differentiation markers, including its own expression and that of Osterix (Osx), in immature osteoblasts. Osx functions downstream of RUNX2, as evidenced by the absence of Osx expression in Runx2-deficient models, which further blocks progression to mature osteoblasts.^[42] In later maturation stages, RUNX2 activates genes encoding extracellular matrix proteins, such as Osteopontin (Spp1) and Bone Sialoprotein (Ibsp), promoting the synthesis of the bone matrix essential for structural integrity.^[41] These targets are directly regulated through RUNX2 binding to OSE2 elements in their promoters, enhancing transcription in committed osteoblasts. RUNX2 facilitates extracellular matrix mineralization by inducing genes like Ibsp, which supports hydroxyapatite deposition and nodule formation in maturing osteoblasts.^[41] Its temporal expression peaks at the transition from proliferation to differentiation, with high levels in proliferating osteoprogenitors that decline as cells exit the cell cycle and commit to matrix production. This dynamic regulation ensures coordinated progression, as sustained RUNX2 activity in early stages drives commitment while its modulation allows maturation.^[43] In vitro studies demonstrate that RUNX2 overexpression in mesenchymal precursor cells, such as C2C12 myoblasts, redirects their fate toward an osteogenic phenotype, inhibiting myogenesis and inducing expression of osteoblast markers like Alkaline Phosphatase and Osteocalcin. Forced expression via viral vectors in these cells enhances alkaline phosphatase activity and mineralized nodule formation, confirming RUNX2's sufficient role in driving osteoblast-like differentiation independently of endogenous cues.

Involvement in Chondrogenesis and Skeletal Development

RUNX2 is essential for chondrogenesis, particularly in promoting the hypertrophy of chondrocytes during cartilage formation. It drives the terminal differentiation of prehypertrophic and hypertrophic chondrocytes by directly inducing the expression of hypertrophy-related genes, such as Col10a1, Mmp13, and Vegfa, which facilitate matrix remodeling and vascularization.^[44] In Runx2-null mice, chondrocyte maturation is severely impaired, resulting in an accumulation of immature chondrocytes, delayed vascular invasion into the cartilage template, and a complete block in endochondral ossification despite normal initial chondrogenesis.^[40] This underscores RUNX2's role in transitioning cartilage to bone by enabling the recruitment of osteoprogenitors and osteoclasts to the hypertrophic zone.^[45] In endochondral ossification, RUNX2 coordinates the chondrocyte-to-osteoblast transition at the growth plate by integrating key signaling pathways. It upregulates Indian hedgehog (Ihh) expression in hypertrophic chondrocytes, which diffuses to the perichondrium to induce parathyroid hormone-related protein (PTHrP) secretion, establishing a negative feedback loop that balances chondrocyte proliferation and differentiation to maintain longitudinal bone growth.^[2] RUNX2 also promotes chondrocyte survival and apoptosis in the terminal hypertrophic zone, ensuring proper scaffolding for osteoblast invasion and mineralization.^[46] Disruption of this regulation in conditional Runx2 knockouts leads to disorganized growth plates and shortened limbs, highlighting its orchestration of the spatiotemporal dynamics in long bone development.^[47] RUNX2 further governs broader skeletal morphogenesis, including cranial suture patency and tooth formation. In calvarial development, it regulates suture closure by inducing Hh, Fgf, Wnt, and Pthlh signaling in mesenchymal cells at the osteogenic fronts, preventing premature fusion while promoting membranous ossification.^[48] Heterozygous Runx2 mutations, as seen in models of cleidocranial dysplasia, result in delayed fontanelle closure and persistent suture patency, illustrating its dosage-dependent control over cranial vault integrity.^[3] Additionally, as of 2025, RUNX2 has been shown to maintain synchondrosis chondrocytes in the cranial base through a FGFR3-MAPK-SOX9 axis, ensuring proper endochondral growth of the skull base.^[49] In odontogenesis, RUNX2 directs root formation by activating Notum (a Wnt inhibitor) and other genes in dental progenitor cells, ensuring proper tooth anchorage.^[50] RUNX2 expression initiates around embryonic day 9.5 (E9.5) in mice, first in the notochord and early mesenchymal condensations, and becomes prominent in limb buds by E11.5, marking the onset of skeletal patterning.^[51] It is indispensable for both endochondral ossification in appendicular skeleton and intramembranous ossification in the cranium and clavicles, where it specifies osteoblast commitment from neural crest-derived mesenchyme without an intervening cartilage stage.^[52] This early and sustained expression ensures coordinated skeletal assembly, with peak activity in hypertrophic zones and osteogenic fronts throughout fetal and postnatal growth.^[53]

Cell Cycle Regulation

RUNX2 plays a critical role in modulating the cell cycle in osteoblasts, primarily by enforcing a G1 phase checkpoint that balances proliferation with differentiation. In preosteoblastic cells, RUNX2 protein levels oscillate during the cell cycle, peaking in early G1 phase and declining in late G1 prior to S-phase entry.^[54] This temporal regulation supports an antiproliferative function, as elevated RUNX2 delays the G1/S transition and promotes exit from active proliferation toward quiescence.^[54] Specifically, RUNX2 upregulates cyclin-dependent kinase inhibitors such as p21CIP1 (CDKN1A) and p27KIP1 (CDKN1B), which inhibit CDK activity and enforce G1 arrest to prevent untimely S-phase progression.^[55] These mechanisms ensure that osteoprogenitors withdraw from the cell cycle at an appropriate stage to commit to osteogenic maturation. RUNX2 further suppresses proliferation by antagonizing key G1/S drivers, including downregulation of Cyclin D1 expression and inhibition of CDK4/6 complexes. In quiescent osteoblasts, RUNX2 elevation correlates with reduced Cyclin D1 levels, disrupting the Cyclin D1-CDK4/6 axis that normally phosphorylates RB to release E2F-mediated transcription of S-phase genes.^[54] Additionally, RUNX2 interacts with the E2F-RB pathway to repress mitotic gene expression, limiting progression beyond G1 and maintaining cells in a differentiation-competent state.^[56] This repressive activity on cell cycle promoters is evident in studies where RUNX2 overexpression in MC3T3-E1 preosteoblasts induces G1 arrest, reducing the S-phase fraction by up to 50% compared to controls.^[54] The osteogenic balance mediated by RUNX2 is highlighted by its dual influence on proliferation: moderate levels support progenitor expansion, while high levels halt it to favor differentiation. Conditional knockout of Runx2 in osteoprogenitors results in hyperproliferation of calvarial cells, as observed in Runx2-/- primary cultures that exhibit faster growth rates than wild-type counterparts due to unchecked G1/S progression.^[57] Conversely, sustained high RUNX2 enforces proliferation suppression, as demonstrated by G1 arrest in overexpression models, underscoring its role in timing the switch from proliferative expansion to terminal osteoblast differentiation.^[58]

Regulation

Transcriptional and Epigenetic Regulation

The expression of the RUNX2 gene is tightly controlled at the transcriptional level by multiple signaling pathways that drive osteoblast differentiation. Bone morphogenetic protein 2 (BMP2) signaling, mediated through Smad1, induces RUNX2 transcription primarily via activation of the proximal P2 promoter in mesenchymal stem cells and immature osteoblasts. This induction involves Smad1 forming complexes with other factors to bind and activate regulatory elements in the P2 promoter region, thereby committing precursor cells to the osteogenic lineage. Similarly, the Wnt/β-catenin pathway enhances RUNX2 gene expression by promoting the nuclear accumulation of β-catenin, which interacts with LEF1 (lymphoid enhancer-binding factor 1) to bind TCF/LEF consensus sites in the proximal RUNX2 promoter, stimulating transcription in osteoprogenitor cells.^[59]^[60]^[61] Transcriptional repression of RUNX2 also plays a critical role in restricting its expression to appropriate cellular contexts and developmental stages. Twist1, a basic helix-loop-helix transcription factor, inhibits RUNX2 transcription by binding to E-box elements in the P2 promoter and interfering with the activity of activating transcription factors through protein-protein interactions. Msx2, a homeobox-containing repressor, suppresses RUNX2 transcriptional activity via direct protein-protein interaction with its DNA-binding domain and inhibits RUNX2 gene expression by binding to its promoter in undifferentiated mesenchymal cells.^[62]^[63]^[64] Additionally, the miR-23a/27a/24-2 microRNA cluster post-transcriptionally represses RUNX2 by targeting conserved sites in its 3' untranslated region (UTR), thereby reducing mRNA stability and translation in non-osteogenic lineages.^[65] Promoter-specific mechanisms further fine-tune RUNX2 expression, reflecting its isoform diversity. The distal P1 promoter, which drives expression of the RUNX2/p57 isoform predominant in mature osteoblasts, is repressed by CCAAT/enhancer-binding protein β (C/EBPβ) through direct binding to a response element at position -591/-576, thereby modulating osteogenic maturation in response to environmental cues. In contrast, the P2 promoter supports the RUNX2/p56 isoform in early progenitors and is less subject to this repression.^[66] Epigenetic modifications provide an additional layer of control, ensuring stable silencing or activation of RUNX2 in specific tissues. During osteogenesis, histone H3 lysine 27 acetylation (H3K27ac) marks accumulate at the RUNX2 promoters, particularly P1, facilitating open chromatin and recruitment of RNA polymerase II to enhance transcription in differentiating osteoblasts. Conversely, DNA hypermethylation at CpG islands in the RUNX2 promoters silences its expression in non-osteogenic tissues, such as fibroblasts or adipocytes, by promoting chromatin compaction and inhibiting transcription factor access; demethylation of these sites correlates with lineage commitment to osteoblasts. These epigenetic changes are dynamically regulated by enzymes like histone acetyltransferases and DNA methyltransferases, integrating extracellular signals to maintain RUNX2's osteoblast-specific expression pattern.^[67]^[18]^[68]

Post-translational Modifications

Post-translational modifications (PTMs) of RUNX2, including phosphorylation, acetylation, ubiquitination, and O-GlcNAcylation, play critical roles in regulating its protein stability, subcellular localization, and transcriptional activity, thereby fine-tuning osteoblast differentiation and bone homeostasis. These modifications occur primarily on serine, threonine, lysine, and other residues within key domains such as the runt homology domain (RHD) and transactivation domain (TAD), influencing RUNX2's interactions with chromatin and its degradation pathways. Recent research has shown that O-GlcNAcylation of RUNX2 mediates Wnt-stimulated bone formation (as of 2024), while SIRT6 promotes nuclear export of deacetylated RUNX2 to inhibit osteogenic transdifferentiation in vascular cells (as of 2024).^[69]^[28] Phosphorylation modifies RUNX2 at multiple serine/threonine sites, often by kinases responsive to growth factor signaling, affecting its stability and nuclear retention. Cyclin-dependent kinases CDK4 and CDK6 phosphorylate RUNX2 at Ser472, promoting its ubiquitination and subsequent proteasomal degradation during the cell cycle, which limits RUNX2 accumulation in proliferating cells. In contrast, extracellular signal-regulated kinase (ERK) phosphorylates Ser301 in the RHD, enhancing RUNX2's DNA-binding affinity, transactivation potential, and protein stability by inhibiting degradation, as observed in BMP2-stimulated osteoblasts. Glycogen synthase kinase 3β (GSK3β) phosphorylates residues such as Ser369, Ser373, and Ser377 in the C-terminal region, leading to inhibition of RUNX2 transcriptional activity and suppression of osteoblast differentiation. Additionally, phosphorylation at Ser451 by cdc2 kinase facilitates nuclear export and cell cycle progression, reducing RUNX2's osteogenic function. These site-specific events collectively balance RUNX2's role in proliferation versus differentiation. Acetylation primarily targets lysine residues in the TAD, modulating RUNX2's transcriptional potency and resistance to degradation. The histone acetyltransferases p300 and CBP, activated by BMP2 signaling, acetylate RUNX2 at multiple lysine residues in the TAD, enhancing its transactivation domain activity and protecting it from ubiquitin-mediated proteolysis, thereby promoting osteoblast gene expression. Conversely, deacetylation by sirtuin 6 (SIRT6), a NAD+-dependent deacetylase, removes these acetyl groups on RUNX2, suppressing its osteogenic activity and osteogenic differentiation in mesenchymal stem cells and vascular smooth muscle cells.^[70] Ubiquitination targets RUNX2 for proteasomal degradation, tightly controlling its short half-life of approximately 2-4 hours in osteoblasts. The E3 ubiquitin ligases Smurf1 and Smurf2 bind to the nuclear matrix targeting signal (NMTS) in RUNX2's C-terminus, catalyzing polyubiquitination at lysine residues and accelerating degradation, which reduces RUNX2 levels and attenuates osteoblast maturation. This process is often primed by prior phosphorylation events, such as those by CDK4/6, linking cell cycle regulation to protein turnover. O-GlcNAcylation, a nutrient-sensitive modification, adds N-acetylglucosamine to serine/threonine residues via O-GlcNAc transferase (OGT), stabilizing RUNX2 and enhancing its activity under hyperglycemic conditions. OGT modifies RUNX2 at sites including Ser32, Ser33, and Ser371, increasing its transcriptional activation of osteoblast markers like alkaline phosphatase by up to 65% and promoting early osteoblast differentiation in bone marrow mesenchymal stem cells.^[71] Inhibition of O-GlcNAcase (OGA) further elevates O-GlcNAc levels on RUNX2, mimicking hyperglycemia and boosting bone formation.^[71]

Co-regulators and Co-factors

RUNX2, a key transcription factor in osteogenesis, relies on the co-factor core binding factor β (CBFβ) to enhance its DNA-binding affinity and transcriptional activity. CBFβ does not bind DNA directly but stabilizes the runt homology domain (RHD) of RUNX2 through heterodimerization, which is indispensable for RUNX2's function, as RUNX2 exhibits no transcriptional activity in the absence of CBFβ. This interaction is critical during skeletal development, where Cbfb knockout leads to severe bone defects similar to those in Runx2-null mice.^[72] Among co-activators, p300 and CREB-binding protein (CBP) interact with RUNX2 to promote histone acetylation at target gene promoters, facilitating chromatin remodeling and gene activation in osteoblasts. These histone acetyltransferases (HATs) are recruited to RUNX2-bound sites on genes like osteocalcin, enhancing transcriptional output during osteoblast differentiation. Additionally, Mastermind-like 1 (MAML1), a co-activator in the Notch signaling pathway, synergizes with RUNX2 independently of Notch to boost its transactivation potential, particularly in promoting osteoblast-specific gene expression.^[73]^[74] Co-repressors such as Groucho/Transducin-like enhancer of split (TLE) proteins modulate RUNX2 by recruiting histone deacetylases (HDACs), leading to chromatin condensation and suppression of target genes. TLE1, for instance, interacts with RUNX2 to repress ribosomal RNA gene transcription in osteoblasts via HDAC recruitment, thereby fine-tuning osteogenic progression. Similarly, zinc finger protein 521 (ZFP521) acts as a co-repressor by binding RUNX2 and inhibiting its activity through HDAC3 recruitment, which delays osteoblast differentiation and regulates bone mass formation.^[75]^[76]^[77] Context-specific modulation occurs through factors like Distal-less homeobox 5 (DLX5), which synergizes with RUNX2 in osteoblasts to co-activate promoters of bone matrix genes, amplifying osteogenic differentiation. Furthermore, Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) exhibit context-dependent effects on RUNX2, switching from co-activation in early osteoblast commitment to inhibition in mature osteoblasts by binding RUNX2 and altering its transcriptional output in response to Hippo pathway signals. Phosphorylation of RUNX2 can influence its affinity for these co-factors, modulating their regulatory impact.^[78]^[43]

Protein Interactions

Key Interacting Partners

RUNX2, a Runt domain-containing transcription factor essential for osteogenesis, forms physical associations with multiple proteins that influence its stability, localization, and DNA-binding capacity. These interactions occur through specific domains, such as the Runt homology domain (RHD), and are critical for RUNX2's role in skeletal development and cellular regulation. Among its core partners, core-binding factor β (CBFβ) forms an obligate heterodimer with RUNX2, enhancing its affinity for DNA and preventing proteasomal degradation by masking the Runt domain from ubiquitin ligases. This heterodimerization is indispensable for RUNX2's transcriptional activity, as evidenced by studies showing that CBFβ knockout disrupts skeletal formation in mice.^[79] In parallel, RUNX2 physically interacts with Smad1 and Smad3, components of the BMP signaling pathway, via its Runt domain, bridging BMP-induced signals to osteoblast-specific gene expression. Co-immunoprecipitation assays confirm this association, which stabilizes RUNX2 on target promoters.^[80] In cell cycle regulation, RUNX2 associates with p53, where their interaction represses p53 transcriptional activity, inhibiting p53-mediated apoptosis and cell cycle arrest in osteosarcoma cells, as demonstrated through co-immunoprecipitation and functional studies.^[81] Conversely, RUNX2 forms an inhibitory complex with Cyclin D1 and CDK4, where CDK4 phosphorylates RUNX2 at serine-472, leading to its ubiquitination and proteasomal degradation, thereby linking cell cycle progression to RUNX2 turnover. This phosphorylation-dependent interaction was mapped using mutagenesis and in vitro kinase assays.^[82]^[83] During development, RUNX2 heterodimerizes with Twist1, a basic helix-loop-helix transcription factor, which inhibits RUNX2's DNA-binding ability by direct association within the nucleus, as shown in co-immunoprecipitation studies from calvarial cells. This interaction fine-tunes cranial suture patency and osteoblast differentiation.^[84] RUNX2 also cooperatively interacts with special AT-rich sequence-binding protein 2 (SATB2), enhancing mutual transcriptional activation on osteoblast genes like osteocalcin, confirmed by chromatin immunoprecipitation and luciferase reporter assays in mesenchymal stem cells.^[85] Additional partners include Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ), effectors of the Hippo pathway, which physically bind RUNX2 to modulate mechanotransduction and osteogenic fate; co-immunoprecipitation in osteoblasts reveals YAP/TAZ recruitment to RUNX2 target sites.^[86]^[87] These associations collectively shape RUNX2's regulatory network, with functional impacts explored in subsequent contexts.

Functional Consequences of Interactions

RUNX2's interaction with components of the BMP signaling pathway, particularly Smad proteins, results in synergistic activation of osteoblast-specific gene expression. BMP-activated Smads, such as Smad1 and Smad5, form complexes with RUNX2 at composite promoter elements, enabling cooperative binding that RUNX2 cannot achieve alone. This synergy enhances the transcription of genes like osteocalcin and alkaline phosphatase by 5- to 10-fold, as demonstrated in mesenchymal precursor cells where co-expression of RUNX2 and Smad5 markedly increases promoter activity and osteoblast differentiation markers compared to RUNX2 expression in isolation.^[88]^[89] The interaction between RUNX2 and p53 allows RUNX2 to repress p53 target genes such as p21^WAF1^, inhibiting G1 phase arrest and apoptosis in response to DNA damage or stress signals. This repression promotes proliferation in cancer cells, particularly in osteosarcoma where RUNX2 overexpression disrupts p53 function to permit unchecked growth.^[90]^[91] Inhibitory interactions, such as with Twist1, block RUNX2's runt homology domain (RHD), preventing DNA binding and thereby delaying osteoblast differentiation during early skeletogenesis. Twist1 directly binds the RHD of RUNX2, inhibiting its transcriptional activity without altering RUNX2 levels, which maintains progenitor proliferation until Twist1 expression declines to allow differentiation onset. Similarly, interaction with Cyclin D1, via Cyclin D1-CDK4 complexes, sequesters RUNX2 in the cytoplasm, promoting its ubiquitination and degradation to suppress osteogenic gene expression and favor cell cycle progression over differentiation.^[92]^[93]^[94] RUNX2 integrates signals from multiple pathways to coordinate skeletal patterning and development. Through interaction with β-catenin in the Wnt pathway, RUNX2 facilitates crosstalk that regulates osteoblast commitment and bone primordia formation, where β-catenin stabilizes RUNX2 activity to pattern skeletal elements during embryogenesis. Likewise, association with MAML1 from the Notch pathway modulates chondrocyte maturation, with MAML1 enhancing RUNX2's transcriptional output to control hypertrophy and extracellular matrix production in growth plate chondrocytes, thereby balancing osteogenesis and chondrogenesis.^[95]^[96]^[74]

Clinical and Pathological Significance

Cleidocranial Dysplasia

Cleidocranial dysplasia (CCD) is a rare autosomal dominant skeletal disorder primarily caused by heterozygous mutations in the RUNX2 gene, leading to haploinsufficiency of this key transcription factor essential for osteoblast differentiation and bone formation. Approximately 60-70% of individuals with a clinical diagnosis of CCD harbor loss-of-function mutations in RUNX2, including nonsense and missense variants predominantly located in the runt homology domain (RHD), which disrupt DNA binding and transcriptional activation. Deletions encompassing the RUNX2 locus account for about 10% of cases, while the remaining cases may involve other variant types such as frameshift, splicing, or in-frame changes, though RUNX2 mutations are identified in roughly two-thirds of patients overall. About one-third of mutations arise de novo, with the remainder inherited from an affected parent. Characteristic symptoms of CCD reflect impaired intramembranous ossification and include hypoplastic or absent clavicles, allowing the shoulders to approximate anteriorly; delayed closure of the fontanelles and cranial sutures, often resulting in a persistent open anterior fontanelle beyond infancy; and various dental anomalies such as supernumerary teeth, delayed eruption of permanent teeth, and retention of primary dentition. Additional features encompass short stature, midface hypoplasia, hypertelorism, and skeletal abnormalities like a narrow thorax, short ribs, and pubic symphysis defects. Craniofacial manifestations, including macrocephaly and bossing of the forehead, further contribute to the phenotype, with variability influenced by the specific RUNX2 variant. At the molecular level, RUNX2 haploinsufficiency reduces the dosage of functional protein, impairing osteoblast proliferation, differentiation, and maturation, particularly in tissues reliant on intramembranous ossification such as the clavicles and calvaria. This leads to defective bone formation and delayed skeletal development. In some variants, particularly C-terminal mutations (e.g., truncating changes in the PST or NMTS domains), paradoxical effects occur: these can enhance RUNX2 transcriptional activity on target promoters, yet disrupt downstream osteogenic differentiation by altering protein stability, localization, or interactions, ultimately delaying terminal maturation of osteoblasts and exacerbating the CCD phenotype. Diagnosis of CCD is confirmed through genetic testing, including sequencing of the RUNX2 gene and array comparative genomic hybridization to detect deletions or duplications. Clinical evaluation incorporates radiographic imaging to visualize skeletal and dental anomalies, alongside multidisciplinary assessment for associated complications like hearing loss or recurrent infections. Treatment is supportive and symptom-focused, involving orthodontic and surgical interventions to manage dental issues (e.g., extraction of supernumerary teeth and orthodontic alignment), orthopedic procedures for skeletal deformities, and craniofacial reconstructions if needed; there is currently no cure for the underlying genetic defect.

Osteosarcoma and Cancer

RUNX2 is overexpressed in approximately 90% of osteosarcoma cases at the protein level, compared to only 20% in normal controls, and this upregulation is associated with advanced tumor stages and higher grades.^[97] In osteosarcoma tumors, elevated RUNX2 levels correlate with poor response to chemotherapy, as demonstrated in analyses where RUNX2 mRNA was significantly higher in tumors with less than 90% necrosis post-treatment.^[98] This overexpression promotes metastasis by upregulating matrix metalloproteinases such as MMP9 and MMP13, which facilitate extracellular matrix degradation and invasive behavior in cancer cells.^[99] Ectopic expression of RUNX2 in osteosarcoma cells drives proliferation and invasion while contributing to apoptosis evasion through interactions with microRNA-34c, part of a compromised p53-miR-34c-RUNX2 regulatory loop that normally suppresses tumor growth.^[100] Experimental evidence from RNA interference studies shows that RUNX2 knockdown via shRNA in osteosarcoma xenografts reduces tumor volume and enhances doxorubicin-induced apoptosis, with increased necrosis observed in treated models.^[101] Beyond osteosarcoma, RUNX2 is upregulated in prostate, breast, and lung cancers, where it facilitates bone metastasis by promoting epithelial-mesenchymal transition (EMT). In these malignancies, RUNX2 represses E-cadherin expression, enabling loss of cell adhesion and enhanced migratory potential, as seen in BMP-2-induced EMT models in lung cancer cells and similar mechanisms in breast and prostate tumors.^[102]^[103]^[104]

Other Associated Disorders

RUNX2 polymorphisms have been implicated in the susceptibility to metabolic bone diseases, particularly osteoporosis. The single nucleotide polymorphism (SNP) rs7771980 (-1025T>C) in the RUNX2 promoter region is associated with reduced bone mineral density (BMD), with the minor homozygote CC genotype showing significantly lower lumbar spine and femoral neck BMD compared to other genotypes.^[105] This SNP reduces RUNX2 expression by altering promoter activity, thereby contributing to increased osteoporosis risk in postmenopausal women.^[106] Additionally, C-carrier individuals (TC + CC) at rs7771980 exhibit a lower risk of vertebral fractures, highlighting the variant's role in modulating bone fragility.^[107] In inflammatory conditions, RUNX2 is upregulated in synovial fibroblasts from patients with rheumatoid arthritis (RA), where it drives osteogenic differentiation and contributes to pathological bone remodeling. During osteogenic induction, RUNX2 expression significantly increases in RA fibroblast-like synovial cells (FLS) by day 21, promoting the expression of osteogenic markers such as alkaline phosphatase (ALP).^[108] This upregulation facilitates the differentiation of a subpopulation of synovial fibroblasts into osteochondrogenic lineages, leading to ectopic bone formation and ankylosis while coexisting with inflammatory bone erosion mediated by matrix metalloproteinases (MMPs).^[109] By enhancing the invasive and pro-osteogenic potential of RA-FLS, elevated RUNX2 exacerbates joint destruction and bone loss in chronic inflammation.^[109] RUNX2 also plays a role in other inflammatory and metabolic disorders affecting bone. In periodontitis, a chronic inflammatory condition leading to dental alveolar bone loss, RUNX2 expression is markedly elevated in gingival tissues of affected patients compared to healthy controls.^[110] This high expression correlates positively with clinical parameters such as probing depth (r = 0.396) and clinical attachment loss (r = 0.435), suggesting that RUNX2 contributes to the pathogenesis by promoting dysregulated osteogenesis and bone resorption in the periodontal ligament.^[110] Furthermore, in diabetes-induced osteopenia, hyperglycemia elevates O-GlcNAcylation levels, which modifies RUNX2 at key serine residues (Ser32, Ser33, Ser371), linking nutrient sensing to impaired osteoblast differentiation.^[71] This post-translational modification disrupts RUNX2 transcriptional activity, reducing alkaline phosphatase expression and contributing to decreased bone mineral density and increased fracture risk observed in type 1 and type 2 diabetes.^[71]^[111] Rare genetic variants in RUNX2 are associated with metaphyseal dysplasia with maxillary hypoplasia and brachydactyly (MDMHB), a skeletal disorder characterized by short stature, brachydactyly, and dental abnormalities. A 105 kb intragenic duplication encompassing exons 3–5 of RUNX2 leads to gain-of-function effects, resulting in elevated protein levels and enhanced transcriptional activity compared to wild-type RUNX2.^[112] This duplication, identified in affected families via SNP genotyping and confirmed by real-time PCR, disrupts normal bone development, particularly in metaphyses, and represents the first reported gain-of-function mutation in RUNX2.^[112]

Therapeutic Implications

Potential Targets and Strategies

Due to the oncogenic role of RUNX2 in cancers such as osteosarcoma and breast cancer, small molecule inhibitors targeting its Runt homology domain (RHD) have emerged as promising therapeutic agents. For instance, CADD522 binds to the RHD of RUNX2, disrupting its DNA-binding affinity and thereby suppressing tumor cell proliferation, migration, and metastasis in preclinical models of breast cancer. These compounds demonstrate efficacy in reducing tumor burden without immediate cytotoxicity to non-cancerous cells, highlighting their selectivity for dysregulated RUNX2 signaling in cancer contexts.^[113] HDAC inhibitors represent another class of pharmacological strategies to disrupt RUNX2's interaction with co-repressor complexes in cancer. Class I HDACs, particularly HDAC1, are essential for RUNX2 transcriptional activity in tumor cells by maintaining repressive chromatin states at target promoters; inhibiting them with agents like MS-275 or vorinostat reduces RUNX2 expression and derepresses pro-apoptotic genes such as p21, leading to decreased cancer cell survival. In osteosarcoma models, HDAC inhibition attenuates RUNX2-mediated invasion by altering co-repressor recruitment, offering a combinatorial approach with chemotherapy to overcome resistance. These inhibitors leverage RUNX2's reliance on HDACs for oncogenic function, providing a mechanism to indirectly target its activity while minimizing direct protein inhibition challenges.^[114]^[115] To counteract bone loss in conditions like osteoporosis, where RUNX2 expression is diminished, activators that enhance its activity through upstream signaling pathways are under investigation. Agents like linarin stimulate the BMP-2/RUNX2 pathway, promoting osteoblast differentiation and mineralization in ovariectomized rodent models of postmenopausal osteoporosis. Gene therapy approaches, including viral delivery of RUNX2-encoding vectors or cell-penetrating fusion proteins, have boosted RUNX2 levels in mesenchymal stem cells, restoring bone density and preventing trabecular loss in preclinical studies. These strategies aim to amplify RUNX2's physiological role in osteogenesis without systemic overexpression risks.^[116]^[117] Genetic editing tools offer precise modulation of RUNX2 for hereditary and neoplastic disorders. In cleidocranial dysplasia (CCD), caused by RUNX2 haploinsufficiency, CRISPR-Cas9 has been used to correct patient-specific mutations in induced pluripotent stem cells (iPSCs), restoring wild-type RUNX2 expression and enabling normal osteoblast differentiation in vitro, with potential for autologous cell therapies. For osteosarcoma, RNA interference targeting RUNX2 mRNA reduces tumor invasion and proliferation in cell lines. These nucleic acid-based interventions provide selective silencing, addressing RUNX2's overexpression in cancer while preserving essential functions.^[118]^[119] Despite these advances, therapeutic targeting of RUNX2 faces significant challenges, particularly isoform specificity and off-target effects on skeletal development. RUNX2 exists in multiple isoforms (e.g., type I, II, and III) with distinct tissue distributions and functions; inhibitors like CADD522 may non-selectively affect all isoforms, potentially disrupting the osteogenic isoform in non-tumor cells while aiming for the pro-metastatic variant in cancer. Off-target modulation risks impairing bone homeostasis, as RUNX2 inhibition in developing skeletons could mimic CCD-like defects, including delayed ossification and reduced mineralization, necessitating delivery systems confined to diseased tissues. Ongoing research emphasizes isoform-specific ligands and conditional editing to mitigate these issues, ensuring therapeutic benefits outweigh skeletal toxicities.^[113]^[120]^[121]

Recent Research Developments

A 2021 study identified Smoc1 and Smoc2 as novel downstream targets of Runx2 in regulating intramembranous and endochondral bone formation, with Bmp2 inducing their expression synergistically with Runx2 to promote osteoblastogenesis.^[122] In 2023, research demonstrated that oxidative stress-induced degradation of RUNX2 via glutathione depletion impairs osteoblast function and contributes to reduced bone formation in aging models, highlighting its role in senescence-associated bone loss.^[123] Recent investigations have elucidated emerging roles for RUNX2 in immune-osteoblast crosstalk, particularly in arthritis.^[124] Additionally, the epigenetic reader BRD4 has been shown to bind active enhancers in cranial neural crest cells, facilitating RUNX2 recruitment and osteoblast differentiation essential for facial bone development.^[125] Therapeutic advances include preclinical evaluations of RUNX2-targeted siRNA delivered via cationic nanogels, which decrease mineralization in osteoblast-like cells by downregulating RUNX2-mediated pathways, though primarily demonstrated in earlier models with ongoing refinements.^[126] Computational approaches, such as Mendelian randomization, have assessed RUNX2's causal association with osteoarthritis risk.^[127] Single-cell RNA sequencing analyses in 2022 revealed genetic signatures of RUNX2 in intermuscular bone formation during zebrafish development, addressing gaps in understanding its isoform-specific dynamics across cell populations.^[128]

References

[1]
Molecular Mechanism of Runx2-Dependent Bone Development - PMC
Runx2 plays a major role in chondrocyte maturation, and Runx3 is partly involved. Runx2 regulates chondrocyte proliferation by directly regulating Ihh ...
[2]
Whole Aspect of Runx2 Functions in Skeletal Development - MDPI
Runx2 has multiple functions in skeletal development, including chondrocyte maturation, osteoblast differentiation, the proliferation of chondrocytes and ...
[3]
RUNX2-modifying enzymes: therapeutic targets for bone diseases
Aug 13, 2020 · RUNX2 is a master transcription factor of osteoblast differentiation. RUNX2 expression in the bone and osteogenic front of a suture is ...
[4]
https://pmc.ncbi.nlm.nih.gov/articles/PMC3005824/
[5]
https://pubmed.ncbi.nlm.nih.gov/38791330/
[6]
RUNT-RELATED TRANSCRIPTION FACTOR 2; RUNX2 - OMIM
RUNX2, also known as RUNT-RELATED TRANSCRIPTION FACTOR 2, is located at 6p21.1 and is associated with cleidocranial dysplasia.
[7]
https://pubmed.ncbi.nlm.nih.gov/40540718/
[8]
q13950 · runx2_human - UniProt
Nov 2, 2001 · RUNX2 is a transcription factor involved in osteoblastic differentiation and skeletal morphogenesis, essential for osteoblast maturation and ...
[9]
The evolution of Runx genes I. A comparative study of sequences ...
Mar 24, 2003 · Runx genes encode proteins defined by the highly conserved Runt DNA-binding domain. Studies of Runx genes and proteins in model organisms ...
[10]
https://omim.org/entry/600211
[11]
Runx2 Protein Expression Utilizes the Runx2 P1 Promoter to ... - NIH
The proximal P2 promoter (Runx2 P2) regulates the type I isoform (designated Runx2-I), which begins with the amino acids MRIPV and is the first Runx2 discovered ...
[12]
Advances in Runx2 regulation and its isoforms - ScienceDirect.com
Further studies have shown that expression of RUNX2 is initiated from two promoters, the distal P1 promoter and the proximal P2 promoter. The alternative use of ...
[13]
Runx2-I Isoform Contributes to Fetal Bone Formation Even in the ...
Runx2 has two isoforms, Runx2-II and Runx2-I, and production of each isoform is controlled by a unique promoter: a distal promoter (P1) and a proximal promoter ...
[14]
DNA methylation of the RUNX2 P1 promoter mediates MMP13 ...
Aug 10, 2017 · Type II isoform transcription is initiated at the distal promoter P1, whilst type I isoform transcription starts at the proximal promoter P2.
[15]
“A Friend Among Strangers” or the Ambiguous Roles of Runx2 - MDPI
In addition, there is evidence of a type III isoform of Runx2 mRNA in mice and rats. It has the same reading frame as the type II isoform, but the ...
[16]
The bone-specific Runx2-P1 promoter displays conserved three ...
The runx2 type-I transcript is driven by the P2 promoter and is expressed in both osteogenic and non-osteogenic mesenchymal tissues (11). During embryonic ...
[17]
Stimulation of the Runx2 P1 promoter by collagen-derived dipeptide ...
Dec 7, 2021 · Runx2 expression is regulated by the distal P1 promoter whose activity produces the N-terminal isoform type II Runx2, such as MASNS type and ...
[18]
Dose-Dependent Effects of Runx2 on Bone Development - PMC - NIH
Distinct promoters, designated P1 and P2, give rise to functionally similar Runx2-II and Runx2-I isoforms. We postulate that this dual promoter gene structure ...
[19]
RUNX2: A Master Bone Growth Regulator That May Be Involved in ...
RUNX2 plays a crucial role in the proliferation and differentiation of osteoblasts, required for bone formation.Runx2: A Master Bone Growth... · Runx Transcription Factors · Regulation Of Runx2 Activity
[20]
Chondrocyte‐Specific RUNX2 Overexpression Accelerates Post ...
Jun 12, 2019 · RUNX2 expression is reported to be upregulated in human OA cartilage and in the articular cartilage of mice following traumatic joint injury.30, ...
[21]
RUNX transcription factors: orchestrators of development
Sep 5, 2019 · Runx2 protein expression utilizes the Runx2 P1 promoter to establish osteoprogenitor cell number for normal bone formation ... adult hair follicle ...
[22]
Expression of Runx2 transcription factor in non-skeletal tissues ...
A large number of in vitro studies have shown that Runx2 is a positive regulator for the expression of bone matrix genes, including type I collagen, osteopontin ...Missing: injury | Show results with:injury
[23]
RUNX2 Isoform II Protects Cancer Cells from Ferroptosis and ... - eLife
P1 and P2 represent promoters. Isoform II is transcribed from P1, while isoform I is transcribed from P2. Exon 5 and 7 are alternative exons. (B-D) The ...<|control11|><|separator|>
[24]
Post-Translational Regulations of Transcriptional Activity of RUNX2
RUNX2 proteins have several functional domains including a glutamine/alanine-rich domain (QA), Runt homology domain (RHD), nuclear localization signal (NLS) ...Missing: architecture | Show results with:architecture
[25]
Two novel RUNX1 mutations in a patient with congenital ...
Apr 3, 2015 · This region is coded on exons 3 through 5, which correlates to amino acids 50–177, and includes three specific DNA binding sites. The C ...
[26]
[PDF] ROLE AND REGULATION OF RUNX2 IN OSTEOGENESIS†
The 'Runt domain' is an evolutionarily conserved domain located at the N-terminal site which derives its name from the fact that the pair rule gene runt in ...
[27]
https://elifesciences.org/reviewed-preprints/99122
[28]
PEBP2/CBF, the murine homolog of the human myeloid AML1 and ...
The functional element 5'-GGCCACA-3' located at positions -66 to 72 differs from the PEBP2/CBF consensus (5'-PuACCPuCA-3') only by an A-to-G transition at ...
[29]
Cooperative binding of DNA and CBFβ to the Runt domain of the ...
A related protein, known as core binding factor β (CBFβ) also binds to the RD to enhance RD–DNA interaction by 6- to 10-fold. Here, we report results from ...
[30]
Disease mutations in RUNX1 and RUNX2 create nonfunctional ...
CBFβ increases the affinity of the RD for DNA by 10‐fold; thus a detectable galactosidase signal in the modified yeast one‐hybrid assay represents a <100‐fold ...
[31]
Genomic Promoter Occupancy of Runt-related Transcription Factor ...
Indeed, of the ∼220 genes that responded to changes in RUNX2 gene expression (using a t test), only 25 genes were also identified by RUNX2 promoter binding.Experimental Procedures · Chip Assays · Runx2 Regulates Genes...<|control11|><|separator|>
[32]
Histone Deacetylase 3 Interacts with Runx2 to Repress the ...
HDAC3 repressed Runx2-induced activation of the osteocalcin promoter and was found associated with the osteocalcin promoter in osteoblasts. Stable suppression ...
[33]
Histone Deacetylase 7 Associates With Runx2 and Represses Its ...
Runx2 is a key regulator of gene expression in osteoblasts and can activate or repress transcription depending on interactions with various co-factors. Based on ...
[34]
Multiple Signaling Pathways Converge on the Cbfa1/Runx2 ...
Interactions with Runx2 are complex involving both binding of distinct components such as AP-1 factors and Smads to separate sites on DNA, direct interactions ...
[35]
Genomic occupancy of Runx2 with global expression profiling ...
Mar 21, 2014 · The Runx2 peaks were partitioned into 42 subsets of Runx2 binding regions (7 clusters with 6 genomic location categories) and it was observed ...Runx2 Dynamically Occupies A... · Genome-Wide Runx2 Occupancy... · Runx2 Binding Functions At...<|control11|><|separator|>
[36]
https://www.jbc.org/article/S0021-9258%2820%2972671-2/fulltext
[37]
Cell signaling and transcriptional regulation of osteoblast lineage ...
Jul 2, 2024 · Runx2 has an activation domain that turns on the genes for osteocalcin and COL1A1 in addition to its conserved DNA-binding domain.
[38]
Runx2 and Runx3 are essential for chondrocyte maturation, and ...
Runx2 and Runx3 are essential for chondrocyte maturation and that Runx2 regulates limb growth by organizing chondrocyte maturation and proliferation.
[39]
Runx2 is essential for the transdifferentiation of chondrocytes into ...
Nov 30, 2020 · Runx2 is an essential transcription factor for osteoblast differentiation and chondrocyte maturation and has been considered to be required for vascular ...
[40]
The functions of Runx family transcription factors and Cbfb in ...
Runx2 is also required for vascular invasion into the mineralized cartilage, which is composed of terminal hypertrophic chondrocytes 13, 14. Runx1 is also ...
[41]
Runx2 is required for hypertrophic chondrocyte mediated ...
These results strongly suggest that RUNX2 regulates apoptosis of hypertrophic chondrocytes during endochondral ossification. ... The increased levels of Ihh and ...
[42]
Runx2 Regulates Endochondral Ossification through Control of ...
We show Runx2 directly regulates a unique set of cell cycle genes Gpr132, Sfn, c-Myb, and Cyclin A1 to control proliferative capacity of chondrocyte.Runx2 Regulates Endochondral... · Runx2 Functions In... · Runx2 Regulates Cell Cycle...
[43]
Runx2 Regulates Cranial Suture Closure by Inducing Hedgehog ...
Mar 15, 2019 · Runx2 Regulates Cranial Suture Closure by Inducing Hedgehog, Fgf, Wnt and Pthlh Signaling Pathway Gene Expressions in Suture Mesenchymal Cells.Missing: skeletal patency
[44]
Runx2 Regulates Mouse Tooth Root Development Via Activation of ...
Runx2 regulates cranial suture closure by inducing hedgehog, Fgf, Wnt, and Pthlh signaling pathway gene expression in suture mesenchymal cells . Hum Mol ...Missing: patency | Show results with:patency
[45]
Mesenchymal Deletion of Histone Demethylase NO66 in Mice ...
Expression of Runx2 is known to begin in the notochord on mouse embryonic day 9.5 (E9. ... QPCR assay. Comparison of mRNA expression of NO66 in limbs of E15.5 ...
[46]
Runx2, a multifunctional transcription factor in skeletal development
Runx2 plays important roles in multiple steps of skeletal development. Runx2 determines the lineage of osteoblasts from multipotent mesenchymal cells.
[47]
Regulation of Skeletal Development and Maintenance by Runx2 ...
In osteoblasts, Runx2 is required for bone formation by inducing the expression of major bone matrix protein genes, including Col1a1 (collagen type I alpha 1), ...
[48]
The Bone-specific Expression of Runx2 Oscillates during the Cell ...
We propose that Runx2 levels and function are biologically linked to a cell growth-related G 1 transition in osteoblastic cells.
[49]
Regulation of Proliferation, Differentiation and Functions of ... - MDPI
Runx2 is essential for osteoblast differentiation and chondrocyte maturation. During osteoblast differentiation, Runx2 is weakly expressed in uncommitted ...
[50]
Regulation of transcription and chromatin structure by pRB - NIH
In addition to inhibiting E2Fs through direct interaction, pRB is also able to actively repress gene transcription mediated by neighboring transcription factors ...
[51]
Terminal osteoblast differentiation, mediated by runx2 and p27 KIP1 ...
Dec 6, 2004 · Introduction of these constructs into 3T3 cells showed that the colony suppression activity of runx2 is due to a G1 cell cycle arrest dependent ...Results · Runx2 Inhibits Cell Growth... · A Role For P27 In Osteogenic...
[52]
Both the Smad and p38 MAPK pathways play a crucial role in Runx2 ...
Oct 17, 2002 · Our results demonstrate that following TGF-beta and BMP induction, both the Smad and p38 MAPK pathways converge at the Runx2 gene to control mesenchymal ...Missing: Smad1 | Show results with:Smad1
[53]
Canonical WNT Signaling Promotes Osteogenesis by Directly ...
Our results provide evidence for direct regulation of Runx2 by canonical WNT signaling and suggest that Runx2 is a target of β-catenin/TCF1 for the stimulation ...
[54]
Complex Interplay between the RUNX Transcription Factors and ...
Wnt induction of Runx2 is not restricted to osteoblasts and direct regulation by β-catenin/LEF1 has also been shown in chondrocytes (Dong et al., 2006). The ...
[55]
TWIST1 binds the RUNX2 gene promoter - Reactome
TWIST1 transcription factor binds the proximal P2 promoter of RUNX2. Binding involves the basic helix-loop-helix (bHLH) domain of TWIST1 and E1-box in the ...Missing: repression | Show results with:repression
[56]
Regulation of the activity of the transcription factor Runx2 ... - PubMed
We have then found that the homeobox protein Msx2 is able to repress the transcription activity of Runx2 by interacting with it. ... Msx2 to interact with Runx2 ...
[57]
A network connecting Runx2, SATB2, and the miR-23a∼27a∼24-2 ...
Here we report that Runx2, a transcription factor essential for osteoblastogenesis, negatively regulates expression of the miR cluster 23a∼27a∼24-2.
[58]
CCAAT/Enhancer Binding Protein β Abrogates Retinoic Acid ...
... C/EBPβ regulates osteoblastogenesis through direct repression of the Runx2 P1 promoter. In vivo, ectopic expression of C/EBPβ reduced Runx2 expression and a ...
[59]
GATA4 Directly Regulates Runx2 Expression and Osteoblast ...
GATA4 binds near the Runx2 promoter and enhancer and helps maintain open chromatin to regulate Runx2 expression leading to bone mineralization.
[60]
Epigenetic Control of the Bone-master Runx2 Gene during ...
Together, these results suggest that although EZH2-mediated H3K27 tri-methylation is an epigenetic component of Runx2/p57 gene silencing in non-osteoblastic ...Chromatin... · Runx2 P1 Proximal Promoter... · Runx2/p57 Expression...
[61]
SIRT6 protects vascular smooth muscle cells from osteogenic ...
Since SIRT6 is a NAD+-dependent deacetylase, we hypothesized that SIRT6 regulated Runx2 through influencing its acetylation status. As shown in IF staining ...Missing: CBP | Show results with:CBP
[62]
O-GlcNAc Modification of the runt-Related Transcription Factor 2 ...
The post-translational modification of Runx2 by phosphorylation, ubiquitinylation, and acetylation modulates its activity, stability, and ...Missing: ubiquitination | Show results with:ubiquitination
[63]
Cbfbeta interacts with Runx2 and has a critical role in ... - PubMed
We demonstrate that Cbfbeta is expressed in developing bone and forms a functional interaction with Runx2, and that Cbfb(GFP) is a hypomorphic allele.Missing: CBFβ co-
[64]
Regulation of the Bone-Specific Osteocalcin Gene by p300 ... - NIH
Regulation of the bone-specific osteocalcin gene by p300 requires Runx2/Cbfa1 and the Vitamin D3 receptor but not p300 intrinsic histone acetyltransferase ...
[65]
MAML1 Enhances the Transcriptional Activity of Runx2 and Plays a ...
Jan 10, 2013 · Because MAML1 is a coactivator of Notch signaling, we investigated whether or not the action of MAML1 on Runx2 was dependent on Notch.
[66]
Runx2 (Cbfa1, AML-3) Interacts with Histone Deacetylase 6 ... - NIH
Runx2 interacts with the transcriptional corepressor proteins mSin3A and TLE/Groucho (25, 33, 51). The conserved amino acids, VWRPY, at the extreme carboxy ...
[67]
Transcriptional corepressor TLE1 functions with Runx2 in epigenetic ...
Mechanistically, depletion of TLE1 relieves Runx2-mediated repression of rRNA genes transcription and selectively increases histone modifications linked to ...
[68]
Zfp521 antagonizes Runx2, delays osteoblast differentiation in vitro ...
Zfp521 binds Runx2 and represses its transcriptional activity, and Runx2 dose-dependently rescues Zfp521's inhibition of osteoblast differentiation.Missing: corepressor | Show results with:corepressor
[69]
Osteoblast-related Transcription Factors Runx2 (Cbfa1/AML3) and ...
In the breast cancer cells, the Runx2 isoforms displayed a unique divergence in regulation of bsp promoter activity. These two isoforms are denoted according to ...
[70]
Requisite roles of Runx2 and Cbfb in skeletal development - PubMed
Cbfb is required for the functions of Runx1 and Runx2. Thus, Runx2/Cbfb heterodimers play essential roles in skeletal development. Publication types. Review ...Missing: primary paper
[71]
Structural Coupling of Smad and Runx2 for Execution of the BMP2 ...
Our results establish the critical residues of RUNX2 for execution and completion of BMP2 signaling for osteoblastogenesis through a mechanism that requires ...
[72]
Runx2 Disruption Promotes Immortalization and Confers Resistance ...
Dec 3, 2007 · Thus, hDRIL binds E2F1 and activates the cyclin E1 promoter (36), whereas repression of BTG2 leads to up-regulation of cyclins D1 and E1 (37).
[73]
Cyclin D1-Cdk4 Induce Runx2 Ubiquitination and Degradation - PMC
In this report, we show that cyclin D1-Cdk4 induces Runx2 phosphorylation, ubiquitination, and proteasome degradation and thereby inhibits differentiation while ...
[74]
Cyclin D1-cdk4 induce runx2 ubiquitination and degradation - PubMed
We show that cyclin D1-Cdk4 induce Runx2 degradation in an ubiquitination-proteasome-dependent manner. Mutagenesis of Runx2 serine-472, a consensus Cdk site, ...Missing: interaction primary paper
[75]
The Multiple Interactions of RUNX with the Hippo–YAP Pathway - PMC
In this review, we discuss the multiple interactions of RUNX with the Hippo–YAP pathway, their shared collaborators in Wnt, TGFβ, MYC and RB pathways.
[76]
Sox2 antagonizes the Hippo pathway to maintain stemness ... - Nature
Apr 2, 2015 · Furthermore, TAZ stimulates osteogenic differentiation through Runx2 activation, whereas YAP suppresses Runx2-mediated gene transcription. In ...<|separator|>
[77]
A program of microRNAs controls osteogenic lineage progression ...
May 31, 2011 · In this study, we directly addressed whether microRNAs (miRNAs) can control the osteogenic activity of Runx2 and affect osteoblast maturation.3′utr Of Runx2 Mrna Is... · Endogenous Runx2 Protein... · Runx2-Targeting Mirnas...
[78]
Runx2 Is a Common Target of Transforming Growth Factor β1 ... - NIH
Treatment with bone morphogenetic protein 2 (BMP-2) not only blocks myogenic differentiation of C2C12 cells but also induces osteoblast differentiation. The ...
[79]
TGF-β and BMP Signaling in Osteoblast Differentiation and Bone ...
BMP-activated Smads interact with Runx2 to induce osteoblast-specific gene expression in C2C12 cells. Smad1 interacts with Runx2 on the promoter of target genes ...
[80]
The RUNX Family Defines Trk Phenotype and Aggressiveness of ...
Collectively, RUNX2 could possibly contribute to neuroblastoma tumorigenesis by collaborating with MYCN as well as by its inhibitory effect on the p53 family ( ...Missing: paper | Show results with:paper
[81]
RUNX Family Participates in the Regulation of p53-Dependent DNA ...
In contrast, when cells receive the repairable DNA damage, p53 induces G1/S and/or G2/M cell cycle arrest through the transactivation of p21 WAF1, p53R2 (p53- ...
[82]
Article A Twist Code Determines the Onset of Osteoblast Differentiation
Here we show that Twist proteins transiently inhibit Runx2 function during skeletogenesis. Twist-1 and -2 are expressed in Runx2-expressing cells throughout the ...
[83]
A Multifaceted Function of the Runt-related Transcription Factor 2 ...
This review discusses the regulatory mechanisms that are critical for Runx2 expression and function, and highlights the dynamic and complex cross-talks.Missing: architecture | Show results with:architecture
[84]
Signaling Networks that Control the Lineage Commitment and ...
... Runx2 in a consensus Cdk site increases the half-life of Runx2 and causes loss of sensitivity to cyclin D1–induced Runx2 degradation. This presents a ...
[85]
Novel Links among Wnt and TGF-β Signaling and Runx2 - PMC - NIH
Jan 21, 2010 · β-Catenin appears to comprise at least one element of the intersection between Wnt and other growth factor systems in bone in response to BMP-2, ...Figure 3 · Wnt Modulates Tgf-β... · Tgf-β Enhances...
[86]
Wnt/β-catenin signaling components and mechanisms in bone ...
Jul 10, 2024 · In this review, we provide an update of Wnt/β-catenin signaling components and mechanisms in bone formation, homeostasis, and diseases.
[87]
RUNX2 and WWOX genes as molecular biomarkers and candidates ...
Dec 8, 2016 · The data reported in the current study show marked elevation of RUNX2 RNA and protein in OS patients compared to normal control (75 & 90%) ...
[88]
Expression analysis of genes associated with human osteosarcoma ...
May 13, 2010 · RUNX2 was the only gene of the 16 to show significant overexpression in tumors that had a poor response to chemotherapy relative to good responders.
[89]
The Runx2 Osteogenic Transcription Factor Regulates Matrix ... - NIH
Thus, our studies suggest a regulatory linkage between Runx2, the expression of metastatic markers (MMP9 and MMP13), and the invasive behavior of cancer cells.
[90]
MicroRNA-34c Inversely Couples the Biological Functions of ... - NIH
Results: Runx2 and p53 levels are inversely correlated in OS. miR-34c, which targets Runx2, is absent in OS and elevated by p53. Conclusion: p53, miR-34c, and ...Runx2 Promoter And 3′-Utr... · Results · Stabilization Of P53...Missing: G1 | Show results with:G1
[91]
Loss of Runx2 sensitises osteosarcoma to chemotherapy-induced ...
Oct 15, 2015 · We observed that overexpression of Runx2 protected OS cells from doxo-induced apoptosis compared with control cells. These experiments ...Allograft In Vivo Tumour... · Knockdown Of Runx2... · C-Myc Influences...<|control11|><|separator|>
[92]
Lung Tumor-associated Osteoblast-derived Bone Morphogenetic ...
Runx2 Is Required for BMP-2-induced Snail Up-regulation and E-cadherin Down-regulation. We next investigated the role of Runx2 on BMP-2-mediated EMT and ...Missing: upregulated | Show results with:upregulated
[93]
RUNX2 and the PI3K/AKT axis reciprocal activation as a driving ...
Jul 25, 2015 · RUNX2 induces EMT, with increased expression of the EMT-related transcription factor SNAI2 and decreased expression of E-cadherin in breast ...
[94]
Runx2 transcriptome of prostate cancer cells - PubMed Central - NIH
Runx2-mediated induction of CSF2 in PCa cells likely contributes to the increased bone turnover in bone metastatic sites, similar to the role of this cytokine ...Missing: lung | Show results with:lung
[95]
Association of a RUNX2 promoter polymorphism with bone mineral ...
Subjects bearing the minor homozygote genotype (CC) at the RUNX2 -1025 T > C SNP (rs7771980) located in P2 showed a significant association with reduced ...
[96]
Promoter 2 -1025 T/C polymorphism in the RUNX2 gene ... - PubMed
Runx2 knockout mice exhibit a complete lack of ossification, while overexpression of this gene in transgenic mice results in an osteoporotic phenotype. Thus, ...<|separator|>
[97]
The association between genetic variants of RUNX2, ADIPOQ and ...
Compared with the TT subjects at rs7771980 in RUNX2, C-carrier (TC + CC) subjects had a lower vertebral fracture risk after adjusting for age, smoking ...
[98]
Osteogenic differentiation of fibroblast-like synovial cells in ...
Aug 29, 2018 · Expression of ALP, runt related transcription factor 2 (RUNX2) was determined by quantitative real-time PCR. ... Expression of ROBO1 in rheumatoid ...
[99]
Osteochondrogenesis derived from synovial fibroblasts in ...
May 1, 2020 · ... Runx2 ... A subpopulation of synovial fibroblasts leads to osteochondrogenesis in a mouse model of chronic inflammatory rheumatoid arthritis.
[100]
The expression of Runx2 in the pathogenesis of periodontitis
The high expression level of Runx2 in the gingiva of patients with periodontitis may be correlated with the pathogenesis of periodontitis.
[101]
RUNX2 O‐GlcNAc modification links osteogenesis and nutrient ...
Decreased bone mineral density and increased risk of fracture in patients with Type I and II diabetes suggest that bone is a nutrient-responsive tissue, ...
[102]
Metaphyseal dysplasia with maxillary hypoplasia and brachydactyly ...
... RUNX2 duplication found in individuals with MDMHB leads to a gain of function. Until now, only loss-of-function mutations have been detected in RUNX2; the ...Missing: variants | Show results with:variants<|control11|><|separator|>
[103]
Characterization of CADD522, a small molecule that inhibits RUNX2 ...
Sep 19, 2017 · The current study defines the effect of CADD522 on breast cancer growth and metastasis, and addresses the mechanisms by which it exerts its anti-tumor activity.Results · Cadd522 Inhibits Runx2... · Cadd522 Inhibition Of...
[104]
A small-molecule inhibitor of the aberrant transcription factor CBFβ ...
AI-10-49 restores RUNX1 transcriptional activity, displays favorable pharmacokinetics, and delays leukemia progression in mice.Missing: RUNX2 | Show results with:RUNX2
[105]
HDACs control RUNX2 expression in cancer cells through ...
Low doses of all HDACi induced no changes in RUNX2 levels or a slight increase in only two cell lines. This result is in line with the known effect of HDAC ...
[106]
HDACs control RUNX2 expression in cancer cells through ...
Aug 8, 2019 · In this study, we showed that Class I HDACs and in particular HDAC1 are required for RUNX2 efficient transcription in cancer.
[107]
International Journal of Molecular Medicine - Spandidos Publications
Feb 18, 2016 · Linarin promotes osteogenic differentiation by activating the BMP-2/RUNX2 pathway via protein kinase A signaling. Authors:.Missing: mimetics | Show results with:mimetics
[108]
Bone‐Targeted Delivery of Cell‐Penetrating‐RUNX2 Fusion Protein ...
Aug 13, 2023 · These findings suggest that HAB‐30Kc19α‐RUNX2 may be a promising candidate for bone‐targeted therapy in osteoporosis. Keywords: bone ...
[109]
Targeted reversion of induced pluripotent stem cells from patients ...
Jan 22, 2018 · Runt-related transcription factor 2 (RUNX2) haploinsufficiency causes cleidocranial dysplasia (CCD) which is characterized by supernumerary ...
[110]
Antisense Oligonucleotides as a Tool for Prolonged Knockdown of ...
Sep 1, 2023 · An antisense oligonucleotide (ASO) technology can achieve RNA knockdown by recruiting RNase H to the RNA-DNA duplexes for nuclear RNA cleavage.Missing: RUNX2 osteosarcoma<|separator|>
[111]
RUNX2 RNA interference inhibits the invasion of osteosarcoma - PMC
In the present study, following RUNX2 interference, the number of cells that passed through the Transwell were decreased significantly, and this decrease ...Missing: xenografts | Show results with:xenografts
[112]
RUNX2 isoform II protects cancer cells from ferroptosis and ... - eLife
Jun 11, 2025 · This article investigates how isoform II of transcription factor RUNX2 promotes cell survival and proliferation in oral squamous cell carcinoma cell lines.Missing: specificity | Show results with:specificity
[113]
RUNX2-modifying enzymes: therapeutic targets for bone diseases
Aug 13, 2020 · RUNX2 expression in the bone and osteogenic front of a suture is crucial for cranial suture closure and membranous bone morphogenesis. In this ...Missing: mimetics | Show results with:mimetics
[114]
Smoc1 and Smoc2 regulate bone formation as downstream ... - PMC
These results suggest that Smoc1 and Smoc2 function as novel targets for Runx2, and play important roles in intramembranous and endochondral bone formation.
[115]
Glutathione limits RUNX2 oxidation and degradation to regulate ...
Jul 11, 2023 · We demonstrate that reducing GSH biosynthesis led to acute degradation of RUNX2, impaired osteoblast differentiation, and reduced bone formation.
[116]
https://www.spandidos-publications.com/10.3892/ijmm.2016.2490
[117]
BRD4 binds to active cranial neural crest enhancers to regulate ...
This BRD4 association is required for RUNX2 recruitment and appropriate osteoblast differentiation. We conclude that BRD4 controls facial bone development ...
[118]
Cationic Nanogel-mediated Runx2 and Osterix siRNA Delivery ...
Dec 2, 2014 · In our current study, we hypothesized that (1) nanogel-mediated siRNA delivery against Runx2 and Osx genes will decrease messenger RNA (mRNA) ...
[119]
New Insights on the Therapeutic Potential of Runt-Related ...
Jun 14, 2024 · High Runx2 expression is linked to increased OA risk, suggesting Runx2 inhibitors may have therapeutic potential for OA treatment.
[120]
Single-cell transcriptomes and runx2b−/− mutants reveal the ...
Aug 2, 2022 · Single-cell transcriptomes and runx2b−/− mutants reveal the genetic signatures of intermuscular bone formation in zebrafishMissing: isoform | Show results with:isoform