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Cucumber mosaic virus

Cucumber mosaic virus (CMV) is a positive-sense single-stranded RNA virus belonging to the genus Cucumovirus in the family Bromoviridae, featuring icosahedral virions approximately 28–30 nm in diameter and a tripartite genome consisting of three RNA segments (RNA1, RNA2, and RNA3).^[1] It possesses the widest known host range among plant viruses, infecting more than 1,200 species across over 100 families, encompassing vegetables (such as cucumbers, tomatoes, peppers, and squash), ornamentals, weeds, and woody plants.^[2] First identified in cucumbers in the early 20th century, CMV is distributed worldwide across temperate, tropical, and subtropical climates, where it causes economically significant diseases through symptoms including leaf mosaics, mottling, stunting, malformations, and reduced yields.^[3]^[4] Transmission of CMV occurs primarily through over 80 species of aphids in a non-persistent manner, where the virus is acquired and inoculated during brief feeding periods of seconds to minutes, facilitated by the viral capsid protein without requiring helper components.^[1] Additional spread happens mechanically via contaminated tools, hands, or sap; through seeds, pollen, or vegetative propagation; and by the parasitic plant dodder (Cuscuta spp.).^[3]^[2] The virus overwinters in perennial weeds, crops like alfalfa, and infected seeds, serving as reservoirs for seasonal epidemics.^[2] CMV induces a range of symptoms depending on the host, viral strain, environmental conditions, and infection timing, such as chlorotic mosaics and ringspots on leaves, fruit deformities (e.g., warts or roughness), flower color breaking, and plant death in severe cases, leading to substantial economic losses in horticultural and vegetable production.^[3]^[2] Management relies on integrated strategies including the use of virus-free certified seeds and transplants, reflective mulches or row covers to deter aphids, weed control to eliminate reservoirs, vector monitoring and insecticide applications, and prompt removal of infected plants, as no curative chemicals exist.^[5] Resistance breeding in crops like cucumbers has identified genes (e.g., cm and cymv) conferring tolerance, though CMV's genetic variability challenges durable control.^[4]

Overview and Taxonomy

Classification

Cucumber mosaic virus (CMV) belongs to the genus Cucumovirus in the family Bromoviridae and the order Martellivirales, within the realm Riboviria, kingdom Orthornavirae, phylum Kitrinoviricota, and class Alsuviricetes.^[6] This placement is based on its tripartite, positive-sense single-stranded RNA genome and shared replicative mechanisms with other members of the family.^[7] CMV isolates are classified into three subgroups—IA, IB, and II—primarily distinguished by nucleotide sequence identities in their genomic RNAs and serological reactivity patterns.^[8] Subgroup I strains (encompassing IA and IB) exhibit greater than 88% sequence identity among themselves, while subgroup II strains show over 96% identity internally but lower similarity to subgroup I; these divisions correlate with differences in symptom severity, with subgroup I generally inducing more virulent effects. Subgroup IA is uniquely associated with the presence of satellite RNAs (330–390 nucleotides long), which do not encode proteins but can modulate viral replication and host symptom expression, such as necrosis in certain plants.^[6] Subgroup IB is predominantly found in Asia, whereas IA and II have global distributions.^[9] Phylogenetically, CMV forms a monophyletic clade within the genus Cucumovirus, closely related to other bromoviruses through conserved RNA-dependent RNA polymerase genes that facilitate genome replication.^[7] Its evolutionary origins involve frequent reassortment among genomic segments and recombination events, contributing to high genetic diversity and adaptation across diverse hosts; analyses of multiple strains reveal radial evolution patterns, with subgroup divergences estimated to have occurred through these mechanisms over evolutionary timescales.^[10]^[11] As of the 2025 International Committee on Taxonomy of Viruses (ICTV) update, the species nomenclature remains Cucumber mosaic virus, with no alterations to its genus or family assignment, reflecting stable taxonomic consensus based on molecular and biological criteria.^[6]

Discovery and History

The Cucumber mosaic virus (CMV) was first described in 1916 as the causal agent of a mosaic disease affecting cucumbers (Cucumis sativus) in the United States, with simultaneous reports by S.P. Doolittle in Michigan and I.C. Jagger in New York detailing symptoms such as leaf mottling, stunting, and fruit deformation.^[4] This initial identification marked CMV as one of the earliest documented plant viruses, highlighting its impact on cucurbit crops and prompting early investigations into its infectious nature through mechanical sap transmission experiments.^[8] In the 1930s, research advanced understanding of CMV's natural spread, with studies confirming aphid-mediated transmission as a primary mode, notably through experiments by H.R. Fulton and E.S. Rackham demonstrating efficient nonpersistent transmission to tobacco hosts by species like Myzus persicae.^[12] These findings established aphids as key vectors, influencing epidemiological models and control strategies for the virus in agricultural settings during the mid-20th century. Subsequent decades saw expanded host range documentation, but molecular insights emerged in the 1970s when the tripartite single-stranded RNA genome structure was elucidated, revealing three genomic RNAs (RNA1, RNA2, RNA3) essential for replication, movement, and encapsidation.^[13] By the 1980s, CMV strains were classified into subgroups (IA, IB, and II) based on serological, biological, and nucleic acid sequence differences, with seminal work by F. Garcia-Arenal and colleagues using hybridization and sequencing to delineate subgroup I and II distinctions that correlated with host specificity and vector efficiency.^[14] This classification framework facilitated targeted research on strain variability and evolution. Recent studies have further refined knowledge of CMV dynamics; for instance, 2023 investigations in Espelette pepper crops confirmed the absence of seed-mediated transmission despite recurrent epidemics, using grow-out tests on over 5,000 seedlings to rule out vertical spread as a factor.^[15] In 2024, molecular characterization identified CMV isolates infecting purple coneflowers (Echinacea purpurea) in China, revealing subgroup IA strains with high sequence identity to known cucurbit isolates and symptoms including leaf mosaic and stunting.^[16] By 2025, reports documented a significant outbreak of subgroup IB CMV in pepper fields in southwestern France, affecting 78% of samples and extending to five new host genera, underscoring the subgroup's increasing prevalence and low genetic diversity in epidemic contexts.^[17]

Hosts and Symptoms

Host Range

Cucumber mosaic virus (CMV) possesses one of the widest host ranges among plant viruses, infecting more than 1,200 species across over 100 families, encompassing both monocotyledons and dicotyledons.^[8] This broad susceptibility spans major agricultural crops, ornamental plants, and weeds, enabling the virus to persist in varied environments and contribute to its global prevalence.^[5] Key host families include Cucurbitaceae, Solanaceae, Fabaceae, Brassicaceae, and Poaceae, among others.^[8] Among agricultural hosts, CMV primarily affects crops in the Cucurbitaceae family, such as cucumbers (Cucumis sativus), melons (Cucumis melo), squash (Cucurbita spp.), and pumpkins (Cucurbita pepo).^[5] In the Solanaceae family, it infects tomatoes (Solanum lycopersicum), peppers (Capsicum spp.), and tobacco (Nicotiana tabacum).^[8] Ornamental hosts include petunias (Petunia spp.), impatiens (Impatiens spp.), Alstroemeria (Alstroemeria spp.), and marigolds (Tagetes erecta).^[18]^[19] Weed and wild hosts, which act as reservoirs, encompass chickweed (Stellaria media) in the Caryophyllaceae family and species of Chenopodium (e.g., C. amaranticolor, C. quinoa) in the Amaranthaceae family.^[20]^[8] Host range variation occurs across CMV subgroups, with both infecting crops and ornamental plants; subgroup I strains predominate and are generally more virulent than subgroup II strains.^[8]^[19] Factors influencing host specificity include viral strain adaptations to particular plant species and interactions driven by viral genes, such as the 2b protein, which modulates virulence and transmission efficiency.^[8]

Disease Manifestations

Cucumber mosaic virus (CMV) infection typically manifests as a range of visible symptoms on infected plants, primarily affecting foliage and overall growth due to disruption of cellular functions. The virus induces characteristic leaf symptoms, including mosaic patterns of light and dark green or yellow areas, mottling, and vein clearing, where veins appear distinctly yellowed against a greener background.^[3]^[21] Additional leaf distortions such as shoestringing (narrowing and filament-like growth of leaves), chlorosis (general yellowing), ringspots, and oak-leaf patterns can occur, often leading to malformed or wrinkled leaves.^[21]^[5] These symptoms arise from the virus's interference with chlorophyll synthesis and pigment distribution in leaf tissues.^[8] Systemic effects of CMV extend beyond leaves to impact plant development and reproductive structures. Infected plants often exhibit stunting, with reduced internode length and overall dwarfing, alongside necrosis in flowers and fruits.^[3]^[8] Fruit deformation is common in susceptible crops like tomatoes, resulting in bumpy, necrotic, or patchily discolored produce with depressed spots that diminish marketability.^[21] Flower necrosis and color breaking further contribute to decreased seed set and yield.^[3] Symptom severity varies by host species, viral strain, plant age, and environmental conditions; for instance, infections in young seedlings tend to be more severe, causing pronounced stunting and mosaic, while older plants or certain weed hosts may show milder or latent symptoms.^[21]^[8] Strains from subgroup II often induce less aggressive responses compared to subgroup I.^[8] At the cellular level, CMV pathogenesis involves cell-to-cell movement facilitated by the 3a movement protein, which alters plasmodesmata to allow viral spread, followed by systemic transport requiring both the 3a protein and capsid protein.^[8] This dissemination culminates in physiological disruptions, including reduced photosynthesis through downregulation of photosystem genes, thylakoid membrane abnormalities, and decreased chlorophyll content, ultimately limiting plant energy production and exacerbating symptom development.^[8]^[22]

Economic Importance

Affected Crops

Cucumber mosaic virus (CMV) primarily affects major agricultural crops within the Cucurbitaceae family, including cucumber (Cucumis sativus), squash (Cucurbita spp.), and melon (Cucumis melo), where it causes significant stunting and reduced fruit set.^[23] In solanaceous crops such as tomato (Solanum lycopersicum), pepper (Capsicum annuum), and eggplant (Solanum melongena), CMV leads to leaf mottling and deformation, compromising plant vigor and harvestable yield.^[23] Leguminous crops like common bean (Phaseolus vulgaris), chickpea (Cicer arietinum), and lupin (Lupinus spp.) are also susceptible, with infections resulting in mosaic symptoms on foliage and pods that diminish seed production.^[23]^[24]^[25] Yield losses from CMV vary by crop and infection timing, but in cucumbers, epidemics can reduce productivity by 10–20% under typical conditions, escalating to near-total loss (up to 100%) in severe, unmanaged outbreaks.^[26]^[5] In tomatoes, the virus primarily impairs fruit quality through uneven ripening and necrosis, leading to marketable yield reductions of 25–50% in major growing regions such as China.^[27] For peppers and beans, similar impacts occur, with stunted growth and distorted pods contributing to 15–45% losses in affected fields.^[28]^[24]^[29] The virus is widespread across Asia, Europe, and the Americas, with high incidence in intensive vegetable production regions of these continents.^[30] It has become increasingly problematic in greenhouse-grown ornamentals, such as chrysanthemum and petunia, where controlled environments facilitate rapid spread via aphids.^[23]^[19] CMV often interacts synergistically with potyviruses, such as zucchini yellow mosaic virus, in co-infected cucurbit crops, amplifying symptom severity and yield reductions beyond those caused by either virus alone.^[31] This interaction enhances viral replication and movement, leading to more devastating disease outcomes in mixed infections.^[32]

Global and Agricultural Impact

Cucumber mosaic virus (CMV) inflicts substantial economic losses on global vegetable production, with yield reductions ranging from 25% to 50% in tomatoes in major growing regions such as China.^[27] In Europe, losses can reach 60% in melons and up to 80% in peppers, particularly in Spain, while tomato crops in Spain and Italy experience up to 80% plant losses across 70% of production areas, escalating to 100% in cases involving necrogenic satellite RNA.^[27] These impacts extend to other high-value crops like cucurbits and ornamentals, contributing to CMV's status as one of the most economically damaging plant viruses worldwide due to its broad host range exceeding 1,200 species.^[4] As a regulated non-quarantine pest in the European Union, CMV is subject to strict controls under directives such as Commission Implementing Directive (EU) 2020/177, which mandates testing and certification for seeds to prevent spread through planting material.^[33] Similar regulations apply across many countries, including requirements for official testing of vegetable seeds to ensure freedom from the virus, thereby mitigating risks to international trade and agricultural exports.^[34] These measures highlight CMV's role in imposing trade restrictions, particularly for seed and propagative materials from affected regions. In developing countries, CMV threatens food security by severely impacting staple vegetables such as tomatoes, peppers, and cucurbits, which are essential dietary components and income sources for smallholder farmers.^[35] Infections lead to reduced yields and poor-quality produce, exacerbating malnutrition and economic vulnerability in regions like sub-Saharan Africa and South Asia where these crops form the backbone of local agriculture.^[36] A notable recent outbreak occurred in 2021–2022 in the Espelette pepper-growing area of southwestern France, where a subgroup IB variant of CMV was detected in 78% of sampled pepper crops and associated weeds, demonstrating the virus's potential for rapid regional spread and underscoring ongoing challenges in European horticulture.^[17]

Transmission and Epidemiology

Vectors and Transmission Modes

The primary vectors of Cucumber mosaic virus (CMV) are aphids, which transmit the virus in a non-persistent, stylet-borne manner. Over 80 aphid species can vector CMV, with the green peach aphid (Myzus persicae) being one of the most efficient and widespread transmitters. Aphids acquire the virus rapidly during brief feeding probes on infected plants, typically within seconds to a minute, and retain it on their mouthparts (stylets) for only minutes to hours before it becomes non-infectious.^[37]^[15]^[38] In addition to aphid transmission, CMV spreads mechanically through contact with infected plant sap, such as via pruning tools, workers' hands, or contaminated equipment during cultivation activities. CMV can also be transmitted by the parasitic plant dodder (Cuscuta spp.), which bridges infected and healthy plants. Seed transmission is rare and strain-dependent; it is generally absent or very low in cucumber, but has been reported at low rates in tomato depending on the cultivar and isolate, and higher rates in certain weeds such as chickweed. Pollen transmission occurs in some susceptible hosts, including squash, where virus-laden pollen can infect flowers during pollination. Unlike some related viruses, CMV does not spread through soil, water, or root grafts.^[3]^[15]^[39]^[2]^[40] Transmission efficiency can vary among CMV subgroups and aphid species, with differences attributed to the viral coat protein, though recent studies show no overall significant differences between subgroups IA, IB, and II.^[41]^[14]

Disease Cycle

The disease cycle of Cucumber mosaic virus (CMV) begins with the entry of the virus into host plants, primarily through mechanical wounding during handling or via aphid vectors that transmit it in a non-persistent, stylet-borne manner.^[4] Upon entry, the virus establishes local infection near the inoculation site before spreading systemically through the vascular tissue, leading to infection of new growth. The latency period, from inoculation to the onset of visible symptoms such as mosaic patterns and chlorosis, typically lasts 4-5 days in young plants under optimal conditions, though it can extend to 7-14 days in older foliage or less favorable environments.^[43]^[5] During this phase, the virus titer increases, leading to stunted growth and yield reductions as the infection progresses.^[4] Overwintering of CMV occurs primarily in perennial and biennial weed hosts, such as common milkweed (Asclepias syriaca) and winter cress (Barbarea vulgaris), which serve as reservoirs for the virus during off-seasons.^[4] Seed transmission is possible but rare, reported in over 40 plant species, including some crops like tomato and legumes, with transmission rates often below 1% in cucurbits.^[43]^[15] Annual cycles are largely driven by fluctuating aphid populations, which facilitate primary infections from overwintering sources and secondary spread within fields.^[44] Temperature significantly influences the speed and severity of the disease cycle, with optimal replication and symptom development occurring at 20-25°C, where the virus multiplies efficiently and aphid activity peaks.^[45] At higher temperatures above 28°C, symptom expression may intensify or resistance in certain hosts like spinach can break down, accelerating the cycle, while cooler conditions below 20°C slow systemic spread and delay onset.^[4]^[43]

Viral Properties

Genome Organization

The genome of Cucumber mosaic virus (CMV) is composed of three positive-sense single-stranded RNA (ssRNA) molecules, designated RNA1, RNA2, and RNA3, which together form a tripartite genome totaling approximately 8.4 kb. Each RNA is encapsidated separately in virions and functions as a messenger RNA. This organization is characteristic of the genus Cucumovirus in the family Bromoviridae.^[13] RNA1, approximately 3.3 kb in length, encodes the multifunctional 1a protein (about 110 kDa), which contains methyltransferase and helicase domains essential for viral replication. RNA2, around 2.9 kb, encodes the 2a protein (about 97 kDa), the RNA-dependent RNA polymerase subunit that forms the replicase complex with 1a, as well as the 2b protein (about 17 kDa) from an overlapping open reading frame, which acts as a viral suppressor of RNA silencing. RNA3, approximately 2.2 kb, encodes the 3a movement protein (about 32 kDa) required for cell-to-cell spread and the coat protein (about 24 kDa), the latter translated from a subgenomic RNA4 (sgRNA4) of roughly 0.9 kb that is transcribed from the 3' region of RNA3.^[13]^[46]^[13] Certain CMV strains, particularly in subgroup II, produce an additional subgenomic RNA (sgRNA4A) from RNA2 to express the 2b protein, though in subgroup I strains, 2b is primarily translated directly from the genomic RNA2. Infections can also lead to the accumulation of defective interfering (DI) RNAs, which arise through template-switching during replication and consist of truncated or rearranged genomic sequences that interfere with helper virus replication. Additionally, some CMV isolates are associated with satellite RNAs (satRNAs), small (about 0.3-0.4 kb), non-coding RNAs that replicate via the viral replicase but lack sequence homology to the CMV genome; these satRNAs can attenuate or exacerbate disease symptoms depending on the strain.^[13]^[13]^[47] CMV displays notable sequence variability, with isolates classified into subgroups IA, IB, and II based on phylogenetic analysis of genomic RNAs. Nucleotide identities range from 73% to 94% across the three RNAs when comparing representative strains from different subgroups, with inter-subgroup divergence typically 20-30% (equating to ~70-80% identity) and higher conservation within subgroups (80-96%). This variability contributes to differences in host range, symptom severity, and vector transmission efficiency among strains.^[13]^[48]

Virion Structure

The virion of Cucumber mosaic virus (CMV) is isometric and non-enveloped, exhibiting icosahedral symmetry with a triangulation number T=3. These particles measure approximately 28-30 nm in diameter, with a maximum dimension of 30.5 nm observed in structural analyses. The capsid is assembled from 180 identical coat protein (CP) subunits arranged in pentameric and hexameric clusters, forming a truncated icosahedral shell that encapsidates the viral genome.^[14]^[49] The CP is a single polypeptide comprising 219 amino acids, with a molecular weight of approximately 24,500 Da, and relies on interactions with the RNA genome for stability. Structural studies reveal three quasi-equivalent CP conformers (A, B, and C subunits) within the icosahedral asymmetric unit, where the A subunit adopts a more extended conformation due to its position at the pentameric clusters, while B and C subunits form the hexameric clusters. The N-terminal region of the CP is flexible and involved in RNA binding, contributing to the overall integrity of the virion.^[14]^[49]^[50] CMV packages its tripartite positive-sense single-stranded RNA genome into separate virion types, with each particle containing a single RNA molecule: RNA 1 in bottom-component (B) virions, RNA 2 in middle-component (M) virions, and RNA 3 in top-component (T) virions. This selective encapsidation ensures efficient genome delivery, with RNA-CP interactions primarily nonspecific but stabilized at the capsid inner surface.^[14] Cryo-electron microscopy (cryo-EM) at 23 Å resolution has revealed key surface features of the CMV virion, including prominent protrusions formed by β-barrel domains and loops on the CP exterior. These surface elements, particularly the conserved βH-βI loop (residues 190-198), project outward and contain charged residues essential for aphid vector transmission by facilitating stylet attachment and retention in the insect mouthparts. High-resolution X-ray crystallography complements these findings, confirming the protrusions' role in exposing epitopes critical for non-persistent transmission.^[51]^[49]^[37]

Replication and Proteins

The replication of Cucumber mosaic virus (CMV), a positive-sense single-stranded RNA virus, relies on the formation of a replicase complex composed of the non-structural proteins 1a and 2a, which are essential for synthesizing both positive- and negative-strand RNA intermediates. The 1a protein, encoded by RNA 1, possesses helicase and methyltransferase domains that unwind RNA and cap the viral genome, respectively, while the 2a protein, encoded by RNA 2, functions as the RNA-dependent RNA polymerase (RdRp) responsible for catalyzing RNA synthesis. This complex assembles in cytoplasmic vesicles derived from the endoplasmic reticulum, forming viral replication factories or spherules where the viral genome is amplified through asymmetric synthesis favoring positive-strand production.^[52]^[53]^[54] CMV encodes several key proteins that orchestrate its lifecycle, with the 1a and 2a proteins central to replication as described, while the 2b protein, also from RNA 2, acts as a potent suppressor of RNA silencing to evade host defenses and exacerbate infection symptoms. The 3a movement protein, encoded by RNA 3, facilitates viral spread, and the coat protein (CP), also from RNA 3, protects the genome. These proteins interact dynamically; for instance, the 1a-2a interaction is indispensable for replicase activity, and phosphorylation events on 2a can modulate replication efficiency.^[52]^[55]^[56] In host plants, CMV proteins engage critical interactions to promote infection: the 2b protein suppresses RNA interference by binding small interfering RNAs and inhibiting Argonaute proteins, thereby allowing unchecked viral accumulation, while the 3a protein enables cell-to-cell movement by targeting plasmodesmata, increasing their size-exclusion limit to permit passage of viral ribonucleoprotein complexes. These mechanisms ensure efficient intracellular replication and intercellular dissemination without disrupting host cell integrity prematurely.^[56]^[57]^[58]^[59]^[60] Variability in the 2b protein sequence among CMV strains significantly influences symptom severity, with certain isoforms enhancing silencing suppression and viral titer, leading to more pronounced disease in susceptible hosts, whereas others result in milder effects due to reduced counter-defense activity. This strain-specific polymorphism in 2b underscores its role as a key determinant of pathogenicity across diverse plant species.^[61]^[62]^[63]

Environmental Factors

Stability and Survival

Cucumber mosaic virus (CMV) virions demonstrate moderate thermal stability, with a thermal inactivation point of 55–70°C for 10 minutes. The virus is also sensitive to pH extremes, maintaining structural integrity and infectivity within a range of pH 5 to 9, with disassembly or loss of function occurring below pH 5 or above pH 9.5.^[64]^[65]^[14] Outside host plants, CMV exhibits limited persistence due to its relative instability. In plant sap at room temperature, infectivity is lost within a few days to hours, though storage at 4°C can extend viability to several months under protected conditions.^[66]^[14] The virus does not survive long in dried plant debris or soil, typically degrading rapidly in such environments, in contrast to more robust viruses like tobacco mosaic virus.^[4] CMV is highly sensitive to ultraviolet (UV) radiation, which quickly inactivates virions upon exposure.^[4]

Influences on Infection

The infectivity and spread of Cucumber mosaic virus (CMV) are significantly modulated by temperature, with optimal conditions for transmission and symptom development occurring between 15°C and 30°C. Within this range, aphid vectors exhibit peak activity, facilitating efficient non-persistent transmission, while viral replication in host plants proceeds rapidly, leading to higher infection rates in susceptible crops like cucumber and tomato.^[67]^[68] Temperatures above 30°C impair viral acquisition by aphids and decrease transmission efficiency, as demonstrated in controlled studies on tobacco and cucumber hosts.^[69] Humidity and wind further influence CMV dynamics by affecting vector behavior and mechanical dissemination. Relative humidity influences aphid populations, with warm, dry conditions often increasing their numbers and enhancing virus transmission.^[4] CMV does not transmit directly through soil, relying instead on above-ground vectors and mechanical means, which limits its persistence in soil environments.^[45] Additionally, exposure to ultraviolet (UV) light in field settings accelerates virion degradation, thereby reducing the virus's survival on plant surfaces and curbing secondary infections.^[70] Emerging models indicate that climate change, particularly rising temperatures in warming regions, could exacerbate CMV spread by extending the activity window for aphid vectors and potentially enhancing viral replication rates in temperate agricultural zones. Elevated CO2 levels may also increase viral accumulation in infected plants. Projections suggest increased infection incidence in cucumber-producing areas, underscoring the need for adaptive monitoring.^[68]^[71]

Detection and Diagnosis

Serological Methods

Serological methods for detecting Cucumber mosaic virus (CMV) rely on antibodies that specifically bind to the viral coat protein, enabling the identification of the virus in infected plant tissues such as leaves and sap. These techniques are widely used for routine screening in agricultural settings due to their simplicity, cost-effectiveness, and ability to process large numbers of samples.^[72]^[73] The enzyme-linked immunosorbent assay (ELISA) is the standard serological method for CMV detection, particularly suited for field and laboratory screening of crops like cucumbers, tomatoes, and ornamentals. In direct antigen-coated plate (ACP)-ELISA or double antibody sandwich (DAS)-ELISA formats, plant sap is applied to wells coated with capture antibodies, followed by detection antibodies conjugated to enzymes like alkaline phosphatase, which produce a colorimetric signal proportional to virus concentration. These assays target the coat protein of intact virions or dissociated subunits, allowing reliable detection in crude extracts. Polyclonal antibodies provide broad detection across CMV strains, while monoclonal antibodies enhance specificity, enabling differentiation between subgroups I and II based on antigenic variations in the coat protein. For instance, mixed ELISA combining polyclonal and monoclonal antibodies has been shown to be more sensitive than traditional polyclonal DAS-ELISA for routine surveys.^[73]^[72]^[74] Lateral flow devices, such as immunochromatographic strip tests, offer rapid on-site detection of CMV without specialized equipment, making them ideal for growers and extension services. These portable strips function like pregnancy tests: a sample of infected sap is applied to a membrane where gold nanoparticle-labeled antibodies capture the viral coat protein, migrating to a test line for visible results within 5-10 minutes. Commercial kits detect all known CMV isolates, including both subgroups, using polyclonal capture and monoclonal detection reagents.^[75] Serological methods generally achieve high sensitivity, detecting CMV in dilutions of infected sap up to 1:50,000, which corresponds to low virus titers in early infections. This level of detection supports timely field interventions, though confirmation with molecular techniques may be needed for ambiguous results.^[76]

Molecular Techniques

Molecular techniques for detecting Cucumber mosaic virus (CMV) primarily rely on nucleic acid-based methods that target the virus's tripartite RNA genome, enabling precise identification, quantification, and strain differentiation in infected plant tissues. These approaches offer higher specificity compared to serological methods by directly amplifying or sequencing viral RNA sequences, which is crucial for distinguishing CMV subgroups IA, IB, and II that vary in host range and symptom severity. Reverse transcription polymerase chain reaction (RT-PCR) remains a cornerstone technique, often targeting conserved regions of RNA3, the segment encoding the movement protein and coat protein, to confirm infection with high sensitivity down to femtogram levels of viral RNA.^[77] Multiplex RT-PCR variants extend this capability by simultaneously detecting CMV subgroups alongside other pathogens, using subgroup-specific primers that produce distinct amplicon sizes for gel electrophoresis-based differentiation; for instance, primers targeting the coat protein gene on RNA3 can discriminate subgroup I from II isolates in a single reaction, reducing diagnostic time and cost in mixed infections. This method has been validated across diverse hosts like tomato and pepper, achieving detection limits of 10^{-3} to 10^{-4} dilutions of infected sap. Next-generation sequencing (NGS) provides a comprehensive alternative for full genome assembly and variant identification, generating de novo assemblies of the ~8 kb CMV genome from total RNA extracts without prior sequence knowledge; studies using Illumina platforms have reconstructed complete tripartite genomes from 14 CMV variants across seven host plants, revealing recombination hotspots in RNA2 and RNA3 that inform epidemiology and resistance strategies.^[78]^[79] Loop-mediated isothermal amplification (LAMP), particularly in reverse transcription format (RT-LAMP), facilitates field-deployable detection without requiring a thermocycler, relying on Bst DNA polymerase and four to six primers to amplify target sequences like the CMV coat protein gene at a constant 60–65°C for 30–60 minutes, yielding visible turbidity or color change via intercalating dyes. This technique detects as few as 10 copies of CMV RNA in banana and cucurbit samples, with results interpretable by naked eye or portable fluorometers, making it ideal for resource-limited settings. Recent advances integrate CRISPR-Cas systems with amplification for enhanced rapidity and subtyping; for example, a NASBA-CRISPR-Cas13a platform combines isothermal RNA amplification with collateral cleavage of reporter molecules upon binding to CMV-specific guide RNAs, achieving attomolar sensitivity for CMV detection with high specificity and minimal matrix effects in plant samples, suitable for point-of-use settings.^[80]^[81]

Management and Control

Cultural and Preventive Measures

Cultural and preventive measures for Cucumber mosaic virus (CMV) primarily involve farm-level practices aimed at reducing the introduction and spread of the virus through sanitation, certified planting materials, weed management, and physical barriers. These strategies focus on breaking the cycle of infection by limiting reservoirs and vectors at the field level.^[3]^[82] Sanitation practices are essential to prevent mechanical transmission and reduce inoculum sources. Infected plants should be promptly rogued—removed and destroyed by burning or deep burial—to eliminate potential reservoirs for aphids and the virus itself.^[2]^[3] Tools and equipment used for pruning or propagation must be disinfected regularly, such as with a 10% bleach solution, to avoid spreading the virus between plants during handling.^[83]^[3] Using certified CMV-free seeds and transplants is a key preventive step, as the virus can be seed-transmitted in some host species. Seed certification programs, including testing via enzyme-linked immunosorbent assay (ELISA) or molecular methods, ensure low virus incidence; for example, many regions mandate such certification for cucurbit seeds to comply with phytosanitary standards.^[2]^[84] Laboratories like those at Oregon State University offer specific mosaic virus testing for cucumber and related crops to support certification.^[85] Weed control targets removal of alternative hosts that serve as reservoirs for CMV, thereby reducing overwintering sites and aphid multiplication. Broadleaf weeds, particularly species in the Chenopodiaceae family such as Chenopodium album (lamb's quarters), are common reservoirs and should be eliminated from fields and surrounding areas through cultivation or mulching to inhibit growth.^[3]^[2] Crop rotation with non-host crops, such as cereals or brassicas, for at least two years helps dilute soil-based reservoirs and disrupts aphid-virus cycles, though its efficacy is limited by the virus's wide host range.^[86] Physical barriers like floating row covers exclude aphids during early growth stages, while reflective mulches (e.g., aluminum-coated plastic) disorient vectors and can delay CMV incidence by up to two weeks in cucurbit crops.^[82] These measures collectively reduce aphid-mediated transmission without relying on chemical interventions.^[87]

Resistance and Breeding Strategies

Host plant resistance to Cucumber mosaic virus (CMV) primarily manifests through two mechanisms: the hypersensitive response (HR), which limits viral spread via localized cell death, and tolerance, where plants exhibit minimal symptoms despite infection. In cowpea (Vigna unguiculata), HR to CMV is elicited by recognition of the viral 2a protein, independent of its replicase activity, resulting in confinement of the virus to initially infected cells.^[88] Similarly, in Chenopodium amaranticolor, HR requires CMV movement beyond the initial infection site, triggering programmed cell death that restricts systemic spread.^[89] Tolerance is prominent in cucumbers (Cucumis sativus), where certain landraces from the Himalayas, such as 'Srinagar Local-I' and 'Paprola Local', maintain yield under infection due to recessive genetic control that suppresses symptom development without fully eliminating the virus.^[90] Breeding efforts for CMV resistance focus on introgressing resistance (R) genes and employing marker-assisted selection (MAS) to develop tolerant varieties. In cucumbers, quantitative trait loci (QTLs) on chromosomes 2 and 6 have been identified that confer recessive resistance, enabling MAS with Kompetitive allele-specific PCR (KASP) markers for efficient introgression into elite lines.^[91] For peppers (Capsicum annuum), the recessive cmr2 gene provides resistance to CMV subgroup I strains by disrupting viral replication, and has been successfully introgressed via traditional breeding combined with MAS to create partially resistant hybrids.^[92] In melons (Cucumis melo), the cmv1 locus, encoding a mutated vacuolar protein sorting 41 (CmVPS41), imparts recessive resistance to subgroup II strains, with new alleles identified for diversifying breeding programs.^[93] Transgenic approaches, particularly RNA interference (RNAi), have enhanced resistance by silencing viral genes. In peppers, transgenic lines expressing small interfering RNAs (siRNAs) targeting the CMV coat protein or replicase genes exhibit delayed symptom onset and reduced viral titers against multiple strains, with improvements reported in the 2020s through stable inheritance in progeny.^[21] Similar RNAi constructs in tomatoes block long-distance CMV movement, conferring near-complete resistance to subgroups I and II.^[94] Challenges in breeding include CMV's genetic variability across subgroups I and II, which often leads to resistance breakdown as strains evolve to evade single R genes.^[21] Recent advances as of 2025 include CRISPR/Cas9 editing of eIF4E1 and eIF4E2 genes in tomato, conferring broad-spectrum resistance to CMV and other viruses by modifying translation initiation factors, enabling durable protection against diverse CMV isolates.^[95] Emerging RNA-based active agents have also been reported to reliably protect plants against CMV as of 2025.^[96]

References

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The Structure of Cucumber Mosaic Virus and Comparison to ... - NIH
Cucumber mosaic virus (CMV) is the type member of the genus Cucumovirus, family Bromoviridae, which infects over 800 plant species and causes economically ...
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Cucumber Mosaic - Wisconsin Horticulture
Feb 29, 2024 · Cucumber mosaic is a viral disease that affects a wide range of fruits, vegetables, herbaceous and woody ornamentals, and weeds.
[3]
Cucumber Mosaic Virus
### Summary of Cucumber Mosaic Virus (CMV)
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Cucumber mosaic virus - American Phytopathological Society
Jan 1, 2009 · CMV infects 1200 species in over 100 plant families and can cause significant economic losses in many vegetable and horticultural crops.Missing: impact | Show results with:impact
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Understanding and Managing Cucumber Mosaic Virus
Mar 4, 2025 · Cucumber mosaic virus is a worldwide disease. It affects a wide range of regions and climates that include temperate, tropical, and subtropical conditions.
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ICTV Virus Taxonomy Profile: Bromoviridae 2025 - PMC - NIH
Jan 31, 2025 · Some strains of cucumber mosaic virus (genus Cucumovirus) support a 330–390 nt satellite RNA that may induce necrosis in tomato, chlorosis in ...Missing: classification | Show results with:classification
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Family: Bromoviridae - ICTV
The family Bromoviridae includes plant viruses with tri-segmented, positive-sense RNA genomes of about 8 kb.
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Cucumber mosaic virus: viral genes as virulence determinants - PMC
In general, subgroup I strains are more virulent than subgroup II strains; most subgroup I strains develop severe symptoms, including mosaic and stunt, whereas ...
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Genetic Structure and Molecular Variability of Cucumber mosaic ...
Subgroups IA and II have a worldwide distribution, while subgroup IB is reported to be principally restricted to Asia [20]. RNA viruses can undergo rapid ...
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Evolutionary History of Cucumber Mosaic Virus Deduced by ... - NIH
In this study 15 strains of CMV whose nucleotide sequences have been determined were used for a complete phylogenetic analysis of the virus.
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Rearrangements in the 5′ Nontranslated Region and Phylogenetic ...
Cucumber mosaic virus (CMV) has been divided into two subgroups based on serological data, peptide mapping of the coat protein, nucleic acid hybridization ...Source Of Sequence Data · Results · Phylogenetic Analyses<|control11|><|separator|>
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Aphid transmission of nonpersistent plant viruses with special ...
Some factors involved in aphid transmission of the cucumber mosaic virus to tobacco. Jour. Agr. Res. 1933. 47:689-704. Horsfall J. L. The effects of feeding ...
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Cucumber mosaic virus, a model for RNA virus evolution - 2001
Jul 7, 2008 · Cucumber mosaic, first described in 1916 (Doolittle, 1916), was one ... Genome organization of CMV. The nucleotide and amino acid ...
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Details of DPV Cucumber mosaic virus and References
... Subgroups IA and IB, in which Subgroup IA strains are more closely clustered than are Subgroup IB strains. Subgroups IA and II appear to be monophyletic ...Cucumber Mosaic Virus · Host Range And... · Table 1. Genetic Mapping Of...
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Absence of Seed-Mediated Transmission of Cucumber Mosaic Virus ...
Oct 27, 2023 · In the past decade, severe epidemics of cucumber mosaic virus (CMV) have caused significant damage to Espelette pepper crops.Missing: historical | Show results with:historical
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Biological and Molecular Characterization of the Cucumber Mosaic ...
This study aimed to explore the biological and molecular characteristics of the cucumber mosaic virus (CMV) infecting the purple coneflowers in China.
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Outbreak of Cucumber Mosaic Virus Subgroup IB in Pepper from the ...
May 21, 2025 · A CMV subgroup IB outbreak was found in Espelette pepper crops, with 78% of pepper samples affected. Five new host genera were identified. The ...Missing: review | Show results with:review
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Petunia (Petunia spp.)-Viruses
Several viruses have been a problem on petunia including cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), beet curly top virus, and impatiens necrotic ...Missing: chickweed | Show results with:chickweed
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Genealogy of Cucumber mosaic virus Isolated from Ornamental ...
CMV from the ornamental species Alstroemeria sp. (AJ131622), Tagetes erecta (AM396983) and Impatiens sp. (DQ 018289) belongs to subgroup II. CMV could not ...
[20]
Cucumber mosaic virus / RHS Gardening
Cucumber mosaic virus (CMV) is one of the most common plant viruses and causes a wide range of symptoms, especially yellow mottling, distortion and stunting.Missing: petunia | Show results with:petunia
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Pepper Crop Improvement Against Cucumber Mosaic Virus (CMV)
Cucumber mosaic virus was first discovered in cucumbers and muskmelons in ... cucumbers in New York, United States in 1916 (Doolittle, 1916). It is now ...
[22]
Cucumber mosaic virus impairs the physiological homeostasis of ...
The results showed that CMV infection affected the photosynthesis of P. notoginseng, caused serious oxidative damage to P. notoginseng and increased the ...
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Cucumber Mosaic Virus : Vegetable : Center for Agriculture, Food ...
Dec 27, 2018 · Cucumber Mosaic Virus (CMV) can cause severe losses in vegetables, legumes, and ornamentals. The virus is distributed worldwide but is particularly prevalent ...Missing: solanaceous | Show results with:solanaceous
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Major viral diseases in grain legumes - PubMed Central - NIH
Of the other viruses, cucumber mosaic virus (CMV) causes significant yield losses in chickpea, reportedly 45%, when CMV incidence reached 75% (Jones et al., ...Missing: solanaceous | Show results with:solanaceous
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Temperate pulse viruses: cucumber mosaic virus - Agriculture Victoria
Jun 10, 2022 · Cucumber mosaic virus (CMV) causes severe disease in lentils, chickpeas and lupins. In areas where large aphid populations occur, crop losses can be high.Missing: affected | Show results with:affected
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[PDF] Cucumber Mosaic Virus in Hawai'i - CTAHR
The pandemic distribu- tion of cucumber mosaic, coupled with the fact that it typically causes 10–20% yield loss where it occurs (although it ... Cucumber mosaic ...
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Cucumber Mosaic Virus Degrades Pepper Fruit Production ...
Mar 19, 2025 · 2023. “Absence of Seed-Mediated Transmission of Cucumber Mosaic Virus in Espelette Pepper Crops Despite Widespread and Recurrent Epidemics.
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(PDF) Transmission Efficiency of Cucumber mosaic virus by Aphids ...
Aug 10, 2025 · Cucumber mosaic virus (CMV) is a major component of the virus complex that has become more pronounced in snap bean in the midwestern and ...
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Biological and Molecular Characterization of Cucumber mosaic ...
A subgroup II isolate from Ocimum sanctum was found closely related to European isolate, EU191025 [24]. The adaptation of the virus to the new environment and ...
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Characterization of Synergy Between Cucumber mosaic virus and ...
ABSTRACT Mixed infections of cucurbits by Cucumber mosaic virus (CMV) and potyviruses exhibit a synergistic interaction. Zucchini squash and melon plants ...
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Visual tracking of viral infection dynamics reveals the synergistic ...
May 4, 2023 · Cucumber mosaic virus (CMV) is one of the most prevalent plant viruses in the world, and causes severe damage to various crops.<|control11|><|separator|>
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[PDF] Top 10 plant viruses in molecular plant pathology
Cucumber mosaic virus (CMV) is the type member of the genus Cucumovi- rus in the family Bromoviridae. CMV particles are icosahedral in shape and. 29 nm in ...
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[PDF] COMMISSION IMPLEMENTING DIRECTIVE (EU) 2020/177 of 11 ...
Feb 11, 2020 · The seed shall also comply with the requirements concerning Union quarantine pests, protected zone quarantine ... Cucumber mosaic virus [CMV000].
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NAME OF THE ORGANISM: Cucumber mosaic virus (CMV000)
2 – Status in the EU: Is this pest already a quarantine pest for the whole EU? No. Presence in the EU: Yes. List of countries (EPPO Global Database):. Austria ...
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Pepper Crop Improvement Against Cucumber Mosaic Virus (CMV)
Dec 10, 2020 · Cucumber mosaic virus (CMV) is a prevalent virus affecting the quality and yield of pepper, resulting in yield losses of greater than 80% during ...Missing: economic | Show results with:economic
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Cucumber mosaic virus of lupins | Grain, pulses and cereal diseases
Jun 10, 2022 · Cucumber Mosaic Virus (CMV) is a very damaging viral disease of lupin crops. It can also infect a large range of non-cereal crops and pastures.
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A Conserved Capsid Protein Surface Domain of Cucumber Mosaic ...
Cucumber mosaic virus (CMV) is an aphid-transmitted virus, the transmission of which has been studied in some detail (33). It is a plus-sense RNA virus with ...Missing: Fulton 1933
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Cucumber mosaic virus and its 2b protein alter emission of host ...
May 3, 2017 · In Arabidopsis and cucurbits, CMV decreases host quality and inhibits prolonged feeding by aphids, which may enhance virus transmission rates.<|control11|><|separator|>
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Studies on the seed transmission of cucumber mosaic virus in ...
Studies on the seed transmission of cucumber mosaic virus in chickweed (Stellaria media) in relation to the ecology of the virus. Wiley. Annals of Applied ...
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Transmission Comparisons of Cucumber Mosaic Virus Subgroup I ...
Apr 16, 2012 · Both NX and AG varied in transmission efficiency by the five aphids, and our transmission results revealed important differences in transmission ...
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Cucumber Mosaic Virus (CMV): Causes, Symptoms, Disease Cycle
Apr 26, 2025 · It can move the virus to the young leaves, stems, flowers, and fruits, which result in severe mosaic symptoms, stunted growth, and fruit ...
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https://www.%5Bresearchgate
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[PDF] Cucumber mosaic virus - PLANT DISEASE
Cucumber mosaic is the most destructive and widespread disease of cucumber and muskmelons ... Primary disease cycles usually start with the virus carried from.
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https://doi.org/10.1016/b978-0-12-394314-9.00013-0
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Cucumber Mosaic Virus - Penn State Extension
Jul 5, 2023 · Cucumber mosaic virus (CMV) was named for one of the first plants in which it was found. However, it can infect a large number of woody and herbaceous plant ...
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Cucumber Mosaic Virus - an overview | ScienceDirect Topics
Cucumber mosaic virus (CMV) is a type of positive-sense ssRNA virus that infects a wide range of plant species, causing symptoms such as chlorotic local ...
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Genetic variability and evolution of the satellite RNA of cucumber ...
Abstract. The genetic structure of populations of cucumber mosaic virus (CMV) satellite RNA (satRNA) and its evolution were analyzed during the course of a CMV ...Missing: RNA1 RNA2
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High-Throughput Sequencing Discloses the Cucumber Mosaic Virus ...
Based on present knowledge, CMV isolates are classified into two major subgroups, termed I and II, according to their serological properties, symptomatology ...
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The Structure of Cucumber Mosaic Virus and Comparison to ...
Amino acid changes in the coat protein of cucumber mosaic virus differentially affect transmission by the aphids Myzus persicae and Aphis gossypii.Virology ...
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Co-existence of chlorosis inducing strain of Cucumber mosaic virus ...
Apr 22, 2021 · Coat protein of CMV comprised of 657 nucleotides putatively translated into 219 amino acids. CMV can be categorized as chlorosis inducing ...
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The Structure of Cucumber Mosaic Virus: Cryoelectron Microscopy ...
May 26, 1997 · The three-dimensional structure of cucumber mosaic virus (CMV) was analyzed at 23 Å resolution by cryoelectron microscopy and image ...
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Cucumovirus ~ ViralZone - Expasy
Replication occurs in viral factories made of membrane vesicles derived from the ER (spherules). A dsRNA genome is synthesized from the genomic ssRNA(+).
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In Situ Localization and Tissue Distribution of the Replication ...
The replication-associated proteins encoded by Cucumber mosaic virus (CMV), the 1a and 2a proteins, were detected by immunogold labeling in two host species ...
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Production of cucumber mosaic virus RNA5 and its role in ...
Feb 5, 2009 · ... RNAs. Viral RNA replication is dependent on efficient interaction between two nonstructural proteins, 1a and 2a, encoded by monocistronic ...
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Differential Selection of Genes of Cucumber Mosaic Virus Subgroups
In addition, CMV can harbor satellite RNAs that do not encode proteins but can affect the symptoms induced by the virus (Kaper and Waterworth 1977; Gonsalves, ...
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Suppression of Antiviral Silencing by Cucumber Mosaic Virus 2b ...
We show that 2b expressed from the CMV genome drastically reduced the accumulation of 21-, 22-, and 24-nucleotide classes of viral small interfering RNAs ( ...
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Cucumber mosaic virus and its 2b RNA silencing suppressor modify ...
Dec 9, 2011 · The cucumber mosaic virus (CMV) 2b protein not only inhibits anti-viral RNA silencing but also quenches transcriptional responses of plant ...
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The 3a protein from cucumber mosaic virus increases the gating ...
The 3a protein, encoded by RNA 3 of cucumber mosaic virus (CMV), is the putative movement protein of viral progeny in infected plants.
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The 3a Protein from Cucumber Mosaic Virus Increases the Gating ...
Nov 1, 1994 · The 3a protein, encoded by RNA 3 of cucumber mosaic virus (CMV), is the putative movement protein of viral progeny in infected plants.
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Movement protein - Cucumber mosaic virus (strain S) (CMV) - UniProt
Transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell ...
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Self‐interaction of the cucumber mosaic virus 2b protein plays a vital ...
The results indicate that symptom severity is related to virus titre, mainly depending on 2b's capability to suppress RNA silencing.Missing: variability | Show results with:variability
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Strain-specific differences in the interactions of the cucumber mosaic ...
Aug 20, 2024 · The cucumber mosaic virus (CMV) 2b protein was among the first discovered viral suppressors of RNA silencing. It has additional pro-viral ...
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(PDF) The Role of the Cucumber mosaic virus 2b Protein in Viral ...
Aug 7, 2025 · The Cucumber mosaic virus (CMV) 2b protein is a counter-defense factor and symptom determinant. Conserved domains in the 2b protein sequence ...
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Details of DPV Cucumber mosaic virus and References
Described by Doolittle (1916) and Jagger (1916). Selected synonyms. Cucumber ... Alfalfa mosaic virus may also be confused with cucumber mosaic virus ...Missing: discovery | Show results with:discovery<|control11|><|separator|>
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II. Stability, dissociation and reassembly of cucumber mosaic virus
Salt-dissociated cucumber mosaic virus failed to reassemble in a normal fashion below pH 5, where insoluble nucleoprotein precipitates were formed, or above ...
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1F15: CUCUMBER MOSAIC VIRUS (STRAIN FNY) - RCSB PDB
1F15 is the structure of Cucumber Mosaic Virus (strain FNY), determined by X-ray diffraction at 3.20 Å resolution. It has a 305-A diameter.
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Prediction of the preservation of freeze-dried cucumber mosaic virus
The survival of two cucumber mosaic virus strains lyophilized in leaves and in protected plant sap was evaluated using an accelerated storage test at 28, 37 and ...
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Cucumber Mosaic Virus Identification and Treatment - Epic Gardening
Aug 19, 2024 · Cucumber mosaic virus is a damaging common disease that affects more than cucumbers. Learn the symptoms and prevention tactics here.
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Climate Change Effects on Cucumber Viruses and Their Management
Plant viruses are a major threat to cucumber yields, causing annual damages that exceed $30 billion across various crops. Unlike other plant pathogens, ...
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Elevated Temperature Can Reduce Cucumber Mosaic Virus ... - NIH
Aug 1, 2025 · The transmission efficiency was markedly reduced at 30°C compared to 25°C, regardless of variations in temperature during virus acquisition and ...Missing: optimal | Show results with:optimal
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[PDF] Study on the Cucumber Mosaic Virus (CMV)
This propagation is influenced by environmental factors, such as temperature, relative humidity and wind velocity, indicating a positive correlation between ...
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Cucumber Mosaic / Cucurbits / Agriculture - UC IPM
The virus is transmitted by many species of aphids and could be seedborne. ... Mulches may need to be removed in the desert areas when summer temperatures are ...<|control11|><|separator|>
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Studies of Ultraviolet Inactivation of Cucumber Mosaic Virus <i>In ...
It has been known for some time that plant viruses are inactivated in vitro by ultraviolet (UV) irradiation (Arthur and Newell, 1929).Missing: soil survival
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Future Scenarios for Plant Virus Pathogens as Climate Change ...
Aug 6, 2025 · Plant viruses cause considerable losses to crops estimated at over $30 billion annually, which are expected to increase with global warming ( ...<|control11|><|separator|>
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Antigenic properties of the coat of Cucumber mosaic virus using ...
The coat protein (CP) of Cucumber mosaic virus (CMV) was characterized by antigen-capture-ELISA using a panel of monoclonal antibodies (mAbs) which were ...
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Monoclonal Antibodies for Detection and Serotyping of Cucumber ...
May 1, 2008 · Two different test procedures were developed: an ELISA with polyclonal and monoclonal antibodies (mixed ELISA) for the routine detection of CMV ...
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ELISA Reagent Set for Cucumber mosaic virus (CMV) - Agdia
Detection Reagent: Monoclonal. Full Validation Report. Analytical Sensitivity. Limit of detection: 1:97,200 dilution of infected tissue (pathogen titer unknown) ...
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ImmunoStrip® for Cucumber mosaic virus (CMV) - Agdia
The Cucumber mosaic virus (CMV) ImmunoStrip® is used to detect the presence of CMV in ornamental and vegetable crops.
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Sensitive Detection of a Plant Virus by Electrochemical Enzyme ...
The detection limit for the purified CMV is 1.0 ng/mL and the highest dilution ratio of the infected leaf sap is 1:5.0 x 10(4). The method is based on coupling ...
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Broad Spectrum Detection of Cucumber Mosaic Virus (CMV) Using ...
Primers complementary to conserved sequences of cucumber mosaic virus (CMV) RNA 3 were designed and reverse transcription-polymerase chain reaction was directly
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Multiplex RT-PCR detection of Cucumber mosaic virus subgroups ...
Multiplex RT-PCR detection of Cucumber mosaic virus subgroups and Tobamoviruses infecting Tomato using 18S rRNA as an internal control · Authors · Affiliation.Missing: RNA3 | Show results with:RNA3
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High-Throughput Sequencing Discloses the Cucumber Mosaic Virus ...
Jun 23, 2022 · In this study, we analyzed the variability of the CMV genome based on 14 fully sequenced CMV variants infecting seven different host plant ...
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Detection of cucumber mosaic virus isolates from banana by one ...
A single-tube, one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of CMV-infected ...
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https://ipm.ucanr.edu/agriculture/dry-beans/cucumber-mosaic/
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Managing Pests in Gardens: Vegetables: Diseases: Mosaic viruses ...
Mosaic viruses cause yellow/green patterns, spread by aphids/leafhoppers. Control by removing symptomatic plants, using reflective mulches, and keeping weeds ...Missing: prevent | Show results with:prevent
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Cucumber Mosaic Virus | USU
Cucumber mosaic virus (CMV) is a cucumovirus, first reported on cucumbers. However, the pathogen can infect other vegetable crops including peppers, spinach, ...<|control11|><|separator|>
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Cucumber Mosaic / Dry Beans / Agriculture - UC IPM
The use of certified disease-free seed may help minimize the incidence of seedborne cucumber mosaic. Avoid growing beans adjacent to alfalfa fields or ...
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NEW Mosaic Virus Testing | Seed Laboratory - Oregon State University
Mosaic Virus detection now available for vegetable, squash, tomato, pepper, cucumber, potato, and other species of seed. Email seedlab@oregonstate.edu for ...
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Cucumber, Squash, Melon & Other Cucurbit Diseases
Jun 6, 2025 · The disease is easily spread by wind. Rotation of crops and seed ... Cucumber mosaic virus (CMV) and Watermelon mosaic virus (WMV).
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Barrier crops as a cultural control measure of non-persistently ...
Barrier crops have been used since the early 1950s as a cultural control strategy for reducing the spread of non-persistently transmitted aphid-borne viruses.
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The 2a protein of Cucumber mosaic virus induces a hypersensitive ...
These findings suggest that the 2a protein of CMV is sufficient to induce HR in cowpea independently of its replicase activity.
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The hypersensitive response to cucumber mosaic virus in ... - PubMed
The hypersensitive response to cucumber mosaic virus in Chenopodium amaranticolor requires virus movement outside the initially infected cell · Authors.
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Natural Resistances to Viruses in Cucurbits - MDPI
All major cucurbits are susceptible to potyviruses. Also, all are hosts of CMV, but infections are more important in melon and cucumber, whereas watermelon ...
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Genomic-Assisted Marker Development Suitable for CsCvy-1 ...
Aug 17, 2021 · We used genomics coupled with the bulked segregant analysis method and generated the MAS-friendly Kompetitive allele specific PCR (KASP) markers suitable for ...Abstract · Materials and Methods · Results · Discussion
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Identification of Cucumber mosaic resistance 2 (cmr2) That Confers ...
Aug 20, 2018 · Our result thus provide genetic and molecular evidence that cmr2 is a single recessive gene that confers to pepper an unprecedented resistance ...Abstract · Introduction · Results · Discussion
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CmVPS41 Is a General Gatekeeper for Resistance to Cucumber ...
Sep 30, 2019 · We have analyzed the variability of the gene CmVPS41 in a set of 52 melon accessions belonging to 15 melon groups, both from the spp melo and the spp agrestis.
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RNAi-Mediated Resistance to Cucumber Mosaic Virus (CMV) in ...
Discover how transgenic tomato plants expressing CMV-specific dsRNA of the replicase gene show resistance to Cucumber mosaic virus.
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Genetic engineering, including genome editing, for enhancing broad ...
Feb 10, 2025 · Developing broad-spectrum resistance (BSR) in crops is a key strategy for controlling crop diseases and ensuring sustainable crop production.