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Microfauna

Microfauna are microscopic animals, generally defined as heterotrophic organisms smaller than 0.1 mm in body size, including protozoans (such as amoebae, flagellates, and ciliates), nematodes, rotifers, and tardigrades, that inhabit diverse environments like soil, freshwater sediments, marine benthos, and biological soil crusts.^[1] These organisms are primarily bacterivores or fungivores, regulating microbial populations through predation and contributing to nutrient mineralization by releasing excess nutrients from microbial biomass. In soil ecosystems, microfauna populations can reach densities of 10^4 to 10^7 individuals per gram of dry soil, particularly in the upper horizons where moisture is available, enhancing decomposition processes and plant nutrient availability.^[1] Aquatic microfauna, such as benthic ciliates and foraminifera, serve as key links in food webs by consuming bacteria, diatoms, and detritus, thereby transferring energy to higher trophic levels. Microfauna exhibit high diversity and abundance, with nematodes often representing a dominant group in soil animal communities of many temperate ecosystems, adapting to microhabitats within water films or pore spaces.^[2] Their ecological significance extends to soil structure improvement through burrowing and grazing activities that promote aggregation and aeration, indirectly supporting agriculture and carbon sequestration.^[1] In arid environments, such as biological soil crusts, microfauna like testate amoebae and nematodes influence nitrogen cycling and microbial community dynamics under fluctuating moisture conditions.^[3] Disturbances like climate change or land use intensification can alter microfauna communities, affecting ecosystem resilience, as evidenced by shifts in protozoan and nematode abundances in response to temperature and precipitation variations.^[4] Overall, microfauna underpin foundational processes in global biogeochemical cycles, underscoring their indispensable role in maintaining biodiversity and soil health.^[5]

Definition and Characteristics

Definition

Microfauna are defined as microscopic animals that exhibit animal-like qualities, characterized by their small size, typically less than 0.1 mm (100 μm) in body length or width, and comprising heterotrophic eukaryotic organisms that primarily consume organic matter or other small organisms.^[1] These organisms are distinguished from larger fauna, such as mesofauna (0.1–2 mm) and macrofauna (>2 mm), by their microscopic scale, which necessitates specialized techniques for observation and study, and from microbiota by their multicellular or complex unicellular structure with motility and predation behaviors akin to animals.^[1] Microfauna commonly includes protozoans (such as amoebae, flagellates, and ciliates) alongside metazoans like nematodes, rotifers, and tardigrades, due to their shared ecological roles and animal-like traits.^[6] The term "microfauna" was first recorded in scientific literature in 1895, but it gained prominence in early 20th-century ecology, particularly in studies of soil biology, to categorize small invertebrates invisible to the naked eye that play subtle yet essential roles in ecosystems.^[7] Pioneering work in soil ecology during this period, building on classifications by researchers like M.S. Gilyarov in the 1940s, formalized the size-based distinctions within soil animal communities, emphasizing microfauna's position as the smallest metazoan group.^[8] This historical development reflected growing recognition of the diversity and functional importance of these minute organisms in natural environments.

Physical Characteristics

Microfauna display diverse morphologies tailored to their diminutive sizes, often featuring soft, flexible bodies or thin cuticles that enhance permeability for essential exchanges. The high surface-to-volume ratio inherent in their microscopic scale—typically ranging from tens of micrometers to a few millimeters—facilitates rapid diffusion of oxygen, nutrients, and waste without specialized respiratory or circulatory systems, a key adaptation for life in thin water films or interstitial spaces. Many are elongated or cylindrical, such as nematodes, which are unsegmented, pseudocoelomate worms with smooth, elastic cuticles and diameters under 500 μm, enabling them to navigate narrow pores.^[9] In contrast, small arthropods among microfauna possess chitinous exoskeletons for structural support, while non-arthropod groups like rotifers exhibit loricae—sclerotized or gelatinous coverings—for protection without rigidity.^[10] Rotifers are commonly pear- or bottle-shaped, with microfaunal representatives measuring less than 0.1 mm (though some species reach up to 1 mm), and tardigrades have compact, barrel-like bodies up to 1.2 mm, but typically under 0.1 mm for those classified as microfauna.^[9] Locomotion adaptations reflect their need to traverse viscous media at small scales, where inertia is negligible and drag dominates. Nematodes employ sinusoidal undulation of their hydrostatic skeleton for propulsion, supplemented by ciliary gliding or adhesive secretions for attachment in sediments.^[9] Rotifers utilize coronas—ciliated structures at the anterior end—for gliding and feeding, generating metachronal waves that propel them at speeds up to several body lengths per second.^[11] Tardigrades rely on four pairs of stumpy legs terminating in claws or discs for slow, deliberate ambulation over substrates, with body volume regulation aiding movement through hydrostatic pressure.^[12] Sensory systems are correspondingly rudimentary, featuring simple chemoreceptors like nematode amphids for detecting gradients or a ventral nerve cord with ganglia in tardigrades for basic coordination; these enable responses to environmental cues without complex brains.^[9] A notable physiological adaptation is tardigrade cryptobiosis, where individuals retract limbs into a protective tun state, reducing metabolic activity to near zero during desiccation or extremes, allowing revival upon rehydration.^[13] Reproductive strategies prioritize rapid population growth in unstable microhabitats, with parthenogenesis—development of unfertilized eggs—common in rotifers and certain nematodes to bypass mate location.^[11] Rotifers often reproduce cyclically via amictic females producing diploid eggs asexually, shifting to mictic females for sexual phases under stress, yielding high offspring numbers; for instance, Philodina roseola averages 22 eggs per female over its lifespan at 1.22 eggs daily.^[14] Nematodes similarly favor parthenogenesis in many free-living species, with females exhibiting elevated fecundity—up to 317 eggs per individual in bacterivorous types—to exploit ephemeral resources.^[15] These traits, combined with direct development and short generation times, underscore microfauna's resilience despite their size constraints.^[16]

Habitats and Distribution

Terrestrial Environments

Microfauna primarily inhabit terrestrial soils, where they form a significant component of the soil biota, with abundances typically ranging from 10^4 to 10^7 individuals per gram of dry soil, predominantly consisting of protists such as naked amoebae and flagellates, alongside nematodes and other micrometazoans.^[17] These populations are heavily influenced by soil physicochemical properties, including moisture content, which regulates active versus dormant states; pH levels, which affect species composition and diversity; and organic matter, which serves as a key energy source and correlates positively with overall microfaunal richness and abundance.^[18]^[19] In addition to bulk soil, microfauna occupy specialized terrestrial niches such as lichens, mosses, leaf litter layers, and sand dunes, where microhabitat conditions like humidity and substrate stability support distinct communities. For instance, free-living nematodes thrive in the rhizosphere—the soil zone surrounding plant roots—due to enhanced nutrient availability and microbial activity, often comprising bacterivores and fungivores that influence root health.^[20] Similarly, tardigrades are commonly found in moss cushions, exploiting the moist, protected environment for feeding on algae and micrometazoans.^[21] Terrestrial microfauna exhibit remarkable adaptations to environmental stresses, particularly desiccation, with species like tardigrades and certain nematodes entering anhydrobiosis—a reversible state of metabolic shutdown allowing survival in dry conditions for extended periods.^[22] Their vertical distribution in soil profiles is uneven, with highest abundances concentrated in the topsoil (0-10 cm layer) where organic matter and moisture are greatest, declining sharply with depth due to reduced oxygen and resources.^[23] This stratification underscores their role in surface-driven processes like nutrient cycling, though detailed mechanisms are explored elsewhere.^[24]

Aquatic Environments

Microfauna inhabit a wide array of aquatic environments, including freshwater systems such as lakes, rivers, and temporary ponds, as well as marine settings like oceans, deep-sea sediments, and interstitial spaces between sediment grains.^[25] In these habitats, microfauna exist primarily as benthic forms dwelling in sediments or as planktonic organisms suspended in the water column, with interstitial microfauna navigating pore spaces in sandy or muddy substrates.^[25] Freshwater habitats like hyporheic zones in streams and lake bottoms support diverse assemblages, while marine environments range from coastal mangroves to hadal trenches exceeding 6,000 meters in depth.^[26]^[25] Key adaptations enable microfauna to thrive in aquatic conditions, including filter-feeding mechanisms for capturing suspended particles and microbes, as seen in rotifers and ciliates, which use ciliary or appendage-based filtration to process water for food.^[25] Buoyancy is maintained through low-density body structures or lipid inclusions, allowing planktonic species like small rotifers to remain afloat in the water column without sinking into sediments.^[27] In benthic and interstitial habitats, elongated, worm-like forms facilitate movement through tight pore spaces, while some nematodes exhibit high anaerobic capacity to survive low-oxygen conditions in deep-sea or anoxic sediments.^[26] Certain nematodes, for instance, produce resilient eggs that endure desiccation in temporary ponds, enabling rapid recolonization upon reflooding.^[28] Distribution patterns of aquatic microfauna vary significantly by habitat type and environmental gradients. Tropical freshwater bodies, such as lakes and rivers, host higher diversity due to stable temperatures and nutrient availability, with rotifers and nematodes often dominating.^[25] In contrast, oligotrophic deep-sea sediments exhibit lower diversity and abundance, decreasing with depth as food resources diminish, though nematodes and foraminiferans persist in upper sediment layers at densities up to 6,400 individuals per 10 cm² in hadal zones.^[26] Salinity gradients strongly influence distribution, with euryhaline nematodes tolerating a wide range from freshwater to hypersaline conditions, facilitating their presence across estuarine and coastal interstitial habitats.^[29] Rotifers proliferate during phytoplankton blooms in lakes, where their grazing adaptations help control algal densities.^[30]

Ecological Roles

Nutrient Cycling

Microfauna, including protozoa and nematodes, serve as key decomposers in soil and aquatic ecosystems by grazing on bacteria, fungi, and organic detritus, thereby facilitating the breakdown of organic matter and the release of essential nutrients like carbon, nitrogen, and phosphorus. This grazing activity accelerates the decomposition process, converting complex organic compounds into simpler forms that plants and microbes can utilize, ultimately supporting primary productivity.^[31] For instance, bacterivorous nematodes and protozoa consume microbial biomass, excreting excess nutrients in mineralized forms that enrich the surrounding environment.^[32] Through predation, microfauna stimulate microbial activity by regulating bacterial and fungal populations, which enhances turnover rates and mineralization of organic matter.^[33] This predatory pressure promotes bacterial regrowth and enzyme production, leading to increased rates of nutrient mineralization; bacterivorous nematodes, for example, can boost net nitrogen availability by up to 25% and phosphorus by 23% compared to nematode-free conditions.^[31] In soils, such interactions result in 20-30% greater nitrogen transfer and mineralization, as microfauna disrupt microbial immobilization and promote the release of ammonium and nitrate.^[34] Additionally, the gut passage of organic particles by microfauna like nematodes aids in fragmentation, increasing surface area for microbial attack and further stimulating decomposition.^[35] Microfauna contribute specifically to phosphorus solubilization by enhancing the activity of phosphate-solubilizing bacteria through grazing, which liberates bound phosphorus from soil minerals and organic residues during digestion and excretion.^[36] In the nitrogen cycle, their role amplifies mineralization, with protists and nematodes accounting for approximately 25% of soil nitrogen mineralization.^[37] For carbon turnover, microfauna integrate into soil food webs, driving the flux of carbon through microbial loops and detrital pathways, where grazing reduces fungal and bacterial carbon immobilization, promoting rapid cycling and CO2 release.^[32] These processes underscore microfauna's pivotal function in maintaining nutrient balances essential for ecosystem health.^[38]

Interactions in Ecosystems

Microfauna play pivotal roles as predators and herbivores within ecosystem food webs, exerting top-down control on microbial populations and facilitating energy transfer across trophic levels. Protozoans, for instance, graze selectively on bacteria, reducing overall bacterial abundance while altering community composition by favoring resilient taxa such as high-G+C gram-positive bacteria like Arthrobacter species.^[39] This predation not only suppresses fast-growing gram-negative bacteria but also enhances microbial diversity by promoting the proliferation of grazing-resistant forms, thereby stabilizing bacterial dynamics in soil and aquatic microcosms. In aquatic systems, rotifers exemplify herbivory by consuming algae in the 2–50 μm size range, including species like Chlorella and Cryptomonas, which positions them as key intermediaries linking primary producers to higher consumers such as small invertebrates. Consequently, rotifers influence algal population dynamics and contribute to the structure of planktonic food chains, where they serve both as grazers and prey, amplifying trophic connectivity. Symbiotic interactions further integrate microfauna into plant-dominated communities, encompassing both mutualistic and parasitic dynamics that modulate ecosystem stability. Free-living nematodes engage in mutualism with plants by preying on deleterious microbes and transporting beneficial rhizosphere organisms via phoresy, which indirectly bolsters plant nutrient uptake and pathogen resistance without direct parasitism. These interactions enhance plant vigor through balanced microbial regulation in the rhizosphere. In contrast, plant-parasitic nematodes establish antagonistic symbioses by penetrating root tissues to feed on cellular contents, eliciting galls or lesions that compromise vascular function and predispose plants to secondary infections. Such parasitism disrupts host physiology, altering resource allocation and community-level plant fitness. Through their activities, microfauna exert broader community effects, including modifications to soil architecture and the propagation of trophic cascades that sustain biodiversity. Nematodes and protozoans contribute to soil structure by navigating and enlarging existing micropores during locomotion and feeding, which promotes aggregation and aeration at fine scales, fostering habitable conditions for other soil biota. In soil micro-food webs, these organisms drive trophic cascades by regulating basal resources like bacteria and fungi, which in turn supports higher diversity across trophic levels and prevents dominance by any single group. For example, intensified predation pressure from microfauna under restored vegetation amplifies cascading benefits, enhancing overall community resilience and functional redundancy.

Classification and Diversity

Major Phyla

Microfauna encompasses a diverse array of small invertebrates, predominantly from several key phyla, with an emphasis on their metazoan composition excluding larger or microbial elements.^[1] The dominant groups include Nematoda, Rotifera, Tardigrada, and certain classes within Arthropoda, reflecting the invertebrate core of microfaunal assemblages.^[40] Nematoda, or roundworms, represent one of the most abundant phyla in microfauna, comprising both free-living bacterivores and detritivores as well as parasitic forms that interact with other organisms.^[1] These unsegmented, elongate animals exhibit a pseudocoelomate body plan, enabling their widespread occurrence in various microenvironments.^[24] Rotifera, commonly known as wheel animals due to their ciliated corona used for locomotion and feeding, form another critical phylum, characterized by their microscopic size and parthenogenetic reproduction in many species.^[41] Tardigrada, or water bears, are renowned for their cryptobiotic resilience, featuring a four-lobed body with eight clawed legs, allowing survival under extreme conditions.^[24] Within Arthropoda, microfaunal representatives include microscopic mites from the subclass Acari and springtails from the class Collembola, alongside crustacean groups such as Ostracoda (seed shrimps with bivalved carapaces), Copepoda (small copepods with segmented bodies), and Cladocera (water fleas with branched antennae).^[1] These arthropod subgroups contribute significantly to microfaunal diversity through their chitinous exoskeletons and varied appendages.^[24] Taxonomically, microfauna definitions sometimes extend to heterotrophic protozoans, such as foraminifera from the class Foraminifera within the supergroup Rhizaria, which possess tests or shells and engage in benthic deposit feeding, though traditional classifications prioritize multicellular invertebrates.^[42] This inclusion highlights the boundary between protozoan and metazoan microfauna, with invertebrates forming the predominant component.^[1] Microfauna phyla trace their origins to ancient evolutionary events, with nematodes emerging approximately 442 million years ago in marine settings as part of the Ecdysozoa clade.^[43] Similarly, tardigrades exhibit deep phylogenetic roots in the Panarthropoda clade from the Cambrian period, while rotifers belong to the Gnathifera clade with ancient origins, underscoring the early diversification of these resilient groups.^[44]

Biodiversity and Abundance

Microfauna represent a highly diverse group of microscopic animals, the majority of which remain undescribed due to challenges in taxonomic identification and sampling. Nematodes alone account for a significant portion of this diversity, with approximately 25,000 to 30,000 species described as of 2013, and as of 2022 around 28,500 valid species, yet projections suggest millions more undescribed species exist across terrestrial and aquatic habitats.^[45]^[46] This vast undescribed diversity underscores the critical role of microfauna in ecosystem processes, though comprehensive inventories are limited by their small size and cryptic lifestyles. Abundance patterns of microfauna vary markedly by habitat, peaking in organic-rich environments like forest litter and agricultural soils where food resources such as bacteria and fungi are plentiful. In these settings, populations can reach up to $10^7 individuals per gram of dry soil, dominated by protozoa and nematodes that thrive on decomposing organic matter. Seasonal fluctuations in abundance are primarily driven by temperature and moisture, with higher densities observed during warmer, wetter periods that enhance microbial activity and resource availability, while drier or colder conditions lead to dormancy or reduced reproduction.^[1]^[47]^[48] Habitat loss poses a severe threat to microfauna biodiversity, particularly through land-use intensification, urbanization, and erosion that degrade soils and sediments, leading to declines in endemic species adapted to specific microhabitats. These disturbances disrupt organic layers essential for microfauna survival, resulting in reduced species richness and altered community structures in affected areas. Conservation efforts focusing on minimizing soil disturbance are vital to mitigate these impacts and preserve microfaunal contributions to ecosystem health.^[49]^[50]

Study and Applications

Methods of Study

Studying microfauna requires specialized sampling techniques to capture these small, often motile organisms from diverse environments without significant disturbance. In terrestrial habitats, soil coring is a common method to collect intact soil samples, typically using cylindrical corers to extract cores of 5-10 cm depth and diameter, preserving the vertical stratification of microfauna communities.^[51] Extraction from these cores often employs dynamic methods like the Baermann funnel, where soil is placed on a mesh over warm water, allowing nematodes and other soft-bodied microfauna to migrate downward due to thermotropism over 24-48 hours, yielding high recovery rates for active individuals.^[52] For arthropod microfauna such as mites and springtails, the Tullgren or Berlese-Tullgren funnel uses a gradual drying process with heat and light to drive organisms from soil or litter into a collecting vial with preservative, typically over 7-10 days for complete extraction.^[53] In aquatic environments, sampling microfauna involves filtration to concentrate organisms from water columns. Water filtration uses fine-mesh filters (e.g., 0.45-10 μm pore size) or pump systems to process large volumes, effectively capturing protozoa, rotifers, and microcrustaceans while minimizing contamination.^[54] Plankton nets with mesh sizes of 20-100 μm are towed or deployed vertically to sample microzooplankton and other pelagic microfauna, providing depth-specific profiles in lakes, rivers, or marine settings.^[55] Identification of microfauna traditionally relies on microscopy for morphological analysis. Light microscopy, including compound and stereomicroscopes at 100-1000x magnification, enables observation of key traits like body shape, appendages, and locomotion in live or preserved specimens, suitable for protozoa and nematodes.^[56] Electron microscopy, such as scanning or transmission types, offers ultrastructural details for taxonomic resolution at the species level, particularly for complex forms like tardigrades.^[57] Molecular tools like DNA barcoding target specific genetic markers (e.g., COI gene for animals) to assign species identities, overcoming limitations of morphology in cryptic taxa.^[58] Metagenomics sequences bulk environmental DNA to profile entire communities, revealing diversity including rare or dormant microfauna without culturing.^[59] Quantitative approaches assess microfauna abundance and function. Biomass estimation often involves counting individuals under microscopy and multiplying by average biovolume or dry weight per taxon, providing metrics in μg/g soil or water.^[56] Community profiling uses quantitative PCR (qPCR) to amplify taxon-specific genes, enabling absolute quantification of groups like nematodes via targeted primers.^[60] Flow cytometry rapidly enumerates cells by light scatter and fluorescence, distinguishing live microfauna like ciliates in aquatic samples with high throughput.^[61]

Human Impacts and Applications

Human activities have profoundly impacted microfauna populations, particularly through agricultural practices that disrupt soil and aquatic habitats. The application of pesticides has been shown to significantly reduce the abundance and diversity of soil microfauna, including nematodes and protozoa, with meta-analyses indicating moderate to strong negative effects across various functional groups even at recommended application rates.^[62] Similarly, intensive tillage practices degrade soil structure and expose microfauna to desiccation and predation, leading to decreased populations of soil-dwelling species such as enchytraeids and mites.^[63] In aquatic environments, climate change exacerbates these pressures by altering temperature regimes and water chemistry, which shifts the distribution of microfauna like rotifers toward poleward migrations or higher elevations, potentially reducing local biodiversity in temperate and tropical zones.^[64]^[65] In agriculture, microfauna serve dual roles as both indicators of soil health and active agents in pest management. Nematode communities, particularly free-living species, act as sensitive bioindicators of soil disturbances from pollution or management practices, with shifts in their trophic structure signaling declines in overall soil quality.^[66] Beneficial entomopathogenic nematodes, such as Steinernema carpocapsae and Steinernema feltiae, are widely applied for biocontrol of soil pests including cutworms, armyworms, and fungus gnat larvae; these nematodes parasitize insects by releasing symbiotic bacteria that cause septicemia, offering an environmentally friendly alternative to chemical insecticides.^[67] Their integration into integrated pest management systems has demonstrated efficacy in reducing pest populations by up to 80% in field trials without harming non-target organisms.^[68] As of 2025, emerging applications of microfauna extend to bioremediation and medical research. Soil microfauna, including collembolans and nematodes, facilitate the degradation and translocation of pollutants like microplastics through ingestion and excretion, enhancing natural attenuation in contaminated sites.^[69] Tardigrades show promise in biomonitoring wastewater, with their extremophile genes being explored for engineering microbes in advanced bioremediation systems targeting heavy metals and organic contaminants.^[70] In medicine, protozoan components of the human gut microbiome are increasingly studied for their roles in modulating immune responses and metabolic health, informing probiotic therapies for conditions like inflammatory bowel disease.^[71]

Comparison with Macrofauna

Microfauna are defined as microscopic soil animals and protists smaller than 0.2 mm in body length, often less than 0.1 mm, while macrofauna encompass larger invertebrates exceeding 2 mm.^[72]^[73] This size disparity profoundly influences their mobility and energy dynamics within soil matrices; microfauna navigate narrow pore spaces (typically 0.01–0.1 mm) via passive diffusion or limited crawling, conserving energy through low locomotion demands but restricting long-distance dispersal, whereas macrofauna rely on active burrowing that requires higher energy expenditure for structural modifications.^[74]^[75] Ecologically, microfauna primarily function as decomposers by grazing on microbial communities, facilitating rapid nutrient mineralization at the microscale without significantly altering soil architecture.^[74] In contrast, macrofauna act as ecosystem engineers, such as earthworms that mix soil layers, enhance aeration, and promote aggregate formation, thereby influencing broader habitat heterogeneity and carbon sequestration on a macroscale.^[74]^[76] While microfauna dominate in benthic and pore-water niches with high population densities, macrofauna exert disproportionate impacts through bioturbation, often focusing on surface or epigeic zones.^[72] Feeding strategies further highlight these distinctions: microfauna predominantly engage in microbial grazing, consuming bacteria, fungi, and protozoa to regulate microbial populations and drive fine-scale organic matter breakdown.^[74] Macrofauna, conversely, employ deposit feeding by ingesting bulk soil and detritus or suspension feeding to capture particulate organic matter, processing larger volumes of material and accelerating litter incorporation into deeper soil profiles.^[74]^[77] These approaches underscore microfauna's role in initial decomposition stages versus macrofauna's in secondary transformation and soil turnover.

Comparison with Microflora

Microfauna and microflora are fundamental components of microscopic ecosystems, differing fundamentally in their biological characteristics. Microfauna encompass small heterotrophic animals that are generally motile and include both unicellular forms, such as protozoa (e.g., amoebae and ciliates), and multicellular organisms, like nematodes and rotifers, which range in size from less than 100 μm in body width. In contrast, microflora consist of prokaryotic microorganisms, such as bacteria and archaea, alongside eukaryotic microbes including autotrophic algae and heterotrophic fungi, which are typically non-motile and lack animal-like traits such as complex locomotion or predation behaviors.^[78]^[79] These groups share overlapping habitats, notably in soil water films and pores, the rhizosphere surrounding plant roots, and within animal digestive tracts like earthworm guts, where conditions support dense microbial communities. Despite this coexistence, microfauna frequently interact with microflora through predation, with microfauna serving as consumers that regulate microfloral populations; for example, bacterivorous nematodes and protozoan amoebae prey on bacteria by engulfing cells via pseudopodia, while fungivorous nematodes graze directly on fungal hyphae, disrupting mycelial networks and facilitating nutrient release.^[80]^[81]^[82] Functionally, microfauna drive active trophic dynamics through predation and grazing, which stimulate microbial turnover and immobilize-release cycles of nutrients like nitrogen and carbon, thereby enhancing soil fertility and ecosystem resilience. Microflora, however, primarily contribute through passive mechanisms, such as bacterial decomposition of organic matter, algal photosynthesis for primary production, and fungal roles in symbiotic nutrient uptake, without the direct consumptive pressure exerted by microfauna. This distinction underscores microfauna's role in top-down control within microbial food webs, complementing microflora's bottom-up foundational processes.^[83]^[84]^[79]

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Results revealed that soil fauna facilitated the transfer of up to 26 % more nitrogen from donor to receptive soil, but phosphorus levels remained unaffected.
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Nematodes and their bacterial prey improve phosphorus acquisition ...
Oct 25, 2022 · Nematode predation enhanced the capability of phosphate-solubilizing bacteria in improving plant performance (aboveground P accumulation and ...
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The mechanisms and collective role of soil microfauna in soil ...
Studies have estimated that about 25% of N mineralized in soil is by activities of protists and nematodes, but such estimates have never been verified in ...
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The use of plant, bacterial and fungal resources in soil food webs of ...
Jul 30, 2025 · Soil food webs, driven by complex interactions among plants, microbes and invertebrates, are crucial for carbon and nutrient cycling in ...
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Microfauna - SARE - Sustainable Agriculture Research and Education
Microfauna are tiny creatures, less than 0.006”(0.16 mm). Microfauna inhabit water films and studying them often requires microbiological techniques.
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MICROFAUNA: What is and examples - Evidence Network
Jun 16, 2025 · Microfauna refers to Microanimal species set that inhabit simultaneously in a certain geographical region.
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The evolution of early Foraminifera - PMC - NIH
The Foraminifera represent one of the most ecologically important groups of marine heterotrophic protists (4). Because of their excellent fossil record, the ...Missing: microfauna definition
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Molecular Timetrees Reveal a Cambrian Colonization of Land and a ...
Mar 4, 2013 · Rota-Stabelli, L.I. Campbell, H. Brinkmann, G.D. Edgecombe, S.J. Longhorn, K.J. Peterson, D. Pisani, H. Philippe, M.J. Telford. A congruent ...
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Nematodes as evolutionary commuters between marine, freshwater ...
Sep 12, 2019 · Nematodes arose during the early Cambrian (525 Mya) in marine habitats (Rota-Stabelli et al., 2013), and bacterivory is the probable ancestral ...
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Nematodes - PMC - NIH
Oct 7, 2013 · Only around 30,000 species have been described, but the number of extant nematode species is estimated to be a million or more. These species ...
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[PDF] Soil ciliates (Protozoa: Ciliophora) from evergreen rain forests of ...
Abstract This paper provides, for the first time, compre- hensive data on alpha diversity of soil ciliates from ever- green tropical and temperate rain ...
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Nonlinear response of soil microfauna network complexity and ... - NIH
Aug 29, 2025 · The nonlinear responses of soil microfauna network complexity and stability were primarily linked to soil temperature, moisture, organic carbon, ...
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Identifying potential threats to soil biodiversity - PMC - NIH
Jun 12, 2020 · After a thorough review of the literature identified on a Web of Science search concerning threats to soil biodiversity (topic search: threat* “ ...
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Soil Fauna Diversity - Function, Soil Degradation, Biological Indices ...
Aug 29, 2012 · Land use changes and the intensification of agriculture also generate severe habitat degradation or destruction of soil biota. The environment ...2. Biodiversity Of Soil... · 3. The Role Of Soil Fauna In... · 4. Diversity Of Soil Fauna...
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Soil Sampling Methods - Soil Ecology Wiki
Mar 31, 2023 · Baermann funnels are useful for measurement of microfauna and microorganism abundance and richness in a soil sample. A funnel with a small mesh ...
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MINI-BAERMANN FUNNEL, A SIMPLE DEVICE FOR CLEANING ...
The Baermann filter method is a long-standing, simple technique for recovering nematodes from soil and charcoal coprocultures.
[52]
[PDF] Chapter 19. Sampling Methods for Soil and Litter Fauna
After field collections, the samples are installed in Berlese-. Tullgren funnels and exposed to a light source for 7–10 days until the sample is dried (Fig.
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A simple approach to water and plankton sampling for water ...
A major problem of studies on the water microflora and microfauna in general is the need for accurate estimates and sampling of the standing crop at various.
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Sampling tools for the marine environment - Coastal Wiki
Aug 20, 2020 · The nets, mounted on a rigid yoke, can be towed from the surface to near the bottom for sampling throughout the water column. In order to obtain ...
[55]
(PDF) Chapter 19. Sampling Methods for Soil and Litter Fauna
Nov 13, 2020 · In this chapter, we review numerous techniques and approaches to evaluate and quantify the influences of soil and litter fauna.
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Development and application of a DNA metabarcoding method for ...
In this study, highly sensitive primer sets using the 18S rDNA region as metabarcoding markers were developed specifically for soil nematode fauna.
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DNA Metabarcoding Methods for the Study of Marine Benthic ...
Here, we review DNA metabarcoding approaches used in studies on marine meiobenthos with the aim of facilitating researchers to make informed decisions.Missing: microfauna | Show results with:microfauna
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qPCR-based assessment of microfaunal indicators of oil for ...
Aug 7, 2025 · Here, we evaluate more specifically the potential of microfauna-specific gene quantification in relation to both petroleum-related discharge ...
[59]
Comparing flow cytometry and microscopy in the quantification of ...
In contrast to microscopy, flow cytometry is a technique capable of rapidly counting aquatic organisms (Hofstraat et al. Citation1990; Joachimsthal et al.
[60]
Pesticide effects on soil fauna communities—A meta‐analysis
Jun 7, 2023 · Our study highlights that pesticide use has significant detrimental non-target effects on soil biodiversity, eroding a substantial part of ...
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Conservation tillage practices affect soil microbial diversity and ...
Aug 2, 2023 · In this study, we hypothesized that tillage decreases microbial diversity and richness, while straw retention increases microbial diversity and ...
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[PDF] Aquatic ecosystems & Global climate change
Increases in water temperatures as a result of climate change will alter fundamental ecological processes and the geographic distribution of aquatic species.
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(PDF) soil microfauna as indicators of soil health - ResearchGate
Oct 29, 2017 · Numerous studies showed that soil free-living nematode communities are among the best biological tools for assessing soil disturbances.
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Steinernema carpocapsae, Beneficial nematode (Sc) - Cornell CALS
S. carpocapsae is particularly effective against caterpillars, including various webworms, cutworms, armyworms, girdlers, some weevils, and wood-borers.
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Biological Control: Using Beneficial Nematodes - UMass Amherst
Dec 20, 2021 · Steinernema feltiae is primarily used against fungus gnat larvae and western flower thrips pupae in the soil. Fungus gnat larvae may be ...
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The forgotten impacts of plastic contamination on terrestrial micro ...
Aug 15, 2023 · This review discusses how soil micro- and mesofauna play a key role in facilitating the (bio-)degradation and movement of MP and NP through soil systems.
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[PDF] Tardigrades in Wastewater Biomonitoring: Microscopic Organisms ...
The heterologous expression of tardigrade-derived genes in target microorganisms offers the potential to develop innovative bioremediation systems capable of ...Missing: microfauna | Show results with:microfauna
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Human Microbiome and Its Medical Applications - PMC
The aim of this review is to summarize recent advances about the roles of the microbiome in certain diseases and the underlying mechanisms, as well as the use ...Missing: microfauna | Show results with:microfauna
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Global distribution of soil fauna functional groups and their ...
Oct 17, 2022 · Microfauna are mostly predators of soil bacteria and fungi (e.g. protists and some nematodes), but some groups of soil microfauna are ...
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[PDF] Soil Biodiversity in California Agriculture - CDFA
Thus, either directly (by grazing and predation) or indirectly (by altering soil environmental conditions), macrofauna exert a strong role in decomposition and.<|separator|>
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(PDF) Feeding habits and multifunctional classification of soil-associated consumers from protists to vertebrates
### Summary of Feeding Habits and Ecological Roles
[72]
[PDF] Feeding habits and multifunctional classification of soil‐associated ...
... macrofauna (invertebrates), and soil- associated vertebrates. We have ... In fact, considering that the mobility of animals in the soil matrix is ...
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[PDF] Soil Fungi and Macrofauna in the Neotropics - USDA Forest Service
Fragmentation is largely a consequence of feeding activity of soil macrofauna and chemical alteration is primarily associated with the activity of bacteria and ...
[74]
Changes in Biomass and Diversity of Soil Macrofauna along a ...
Jan 14, 2022 · We classified soil macrofauna (Data S1) into four trophic groups [8]: herbivores (feed on live plant tissues, mainly roots), predators (feed on ...Missing: 1941 | Show results with:1941
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https://edepot.wur.nl/562886
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Soil Microflora - an overview | ScienceDirect Topics
Soil microflora is a diverse community of microorganisms in soil, including bacteria, archaea, viruses, and fungi, that play crucial roles in nutrient cycling.
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Gut Microflora and Microfauna of Earthworm Species in the Soils of ...
Isolated microflora included bacteria, yeasts and moulds while microfauna identified were protozoa and nematodes. For all samples of soil, casts and earthworms, ...
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Exploring the microbial savanna: predator-prey interactions in the soil
Apr 8, 2024 · (A) An ameba can stretch its thin pseudopodia between micropores to reach its bacterial prey, attracted by chemotactic cues produced by the ...
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Nematode grazing increases the allocation of plant-derived carbon ...
Increased grazing through the addition of nematodes most likely results in higher carbon transfer to soil food webs via bacterial and fungal channels.
[80]
Nematodes and Microorganisms Interactively Stimulate Soil Organic ...
Nov 26, 2018 · The intra-aggregate architecture of soil macroaggregates provides suitable microhabitats for nematodes to graze on microorganisms.
[81]
Predation increases multiple components of microbial diversity in ...
Dec 1, 2021 · For example, protozoa may excrete growth-stimulating substances that boost bacterial activity [4]. Predation plays also an important role ...