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Bromethalin

Bromethalin is a synthetic neurotoxic rodenticide designed to kill rats and mice through disruption of their central nervous system, providing lethality from a single feeding dose in targeted species.^[1] It is formulated as a pale, odorless crystalline powder with the chemical name N-methyl-2,4-dinitro-N-(2,4,6-tribromophenyl)-6-(trifluoromethyl)benzenamine and the molecular formula C₁₄H₇Br₃F₃N₃O₄, typically incorporated into baits at concentrations of 0.01% to 0.025% (0.1–0.25 mg/g).^[2]^[3] As a non-anticoagulant alternative, it targets warfarin-resistant rodent populations and is applied in residential, agricultural, and structural settings, including bait stations and loose pellets.^[4] Developed in the mid-1970s by American Cyanamid Company, bromethalin emerged as a response to growing resistance against first-generation anticoagulants like warfarin, achieving approximately 90% efficacy against resistant strains.^[1]^[3] It gained widespread use in the United States starting in the mid-1980s and became the most common rodenticide following the U.S. Environmental Protection Agency's (EPA) 2008 risk mitigation measures that restricted second-generation anticoagulants for consumer use.^[1]^[5] These regulations classified bromethalin as a restricted-use pesticide in many formulations, limiting its availability to certified applicators while allowing certain consumer products with tamper-resistant stations; however, it has been banned in the European Union since 2008.^[6]^[7] The compound's mechanism involves bioactivation in the liver to desmethylbromethalin, which uncouples mitochondrial oxidative phosphorylation, thereby depleting cellular adenosine triphosphate (ATP) and impairing the sodium-potassium pump function.^[1]^[4] This leads to osmotic dysregulation, cerebral edema, increased intracranial pressure, and demyelination of nerves, resulting in paralysis and respiratory failure typically within 24–36 hours in rodents, though effects can be delayed up to several days.^[3]^[4] Bromethalin exhibits high toxicity to non-target mammals, with median lethal doses (LD₅₀) of 0.4–0.71 mg/kg in cats (highly sensitive) and 2.38–5.6 mg/kg in dogs (moderately sensitive), causing dose-dependent syndromes such as acute convulsions (tremors, seizures) or delayed paralysis (lethargy, ataxia).^[1]^[3] Human exposures are rare but can result in severe neurological effects including coma and cerebral edema, with no specific antidote available; treatment relies on decontamination, osmotic diuretics like mannitol, and supportive care.^[4] Environmental concerns include secondary poisoning in wildlife, such as birds of prey, prompting ongoing EPA evaluations of its ecological impacts.^[8]

Chemical properties

Structure and formula

Bromethalin is a synthetic organohalogen compound classified as a substituted diphenylamine, featuring a central N-methyl nitrogen atom linking two aromatic rings with multiple halogen and nitro substituents.^[9] Its molecular formula is C_{14}H_7Br_3F_3N_3O_4, and the molar mass is 577.93 g/mol.^[9] The preferred IUPAC name for bromethalin is N-methyl-2,4-dinitro-N-(2,4,6-tribromophenyl)-6-(trifluoromethyl)aniline.^[9] Structurally, it consists of a 2,4,6-tribromophenyl group connected via the N-methyl bridge to a benzene ring bearing nitro groups at the 2- and 4-positions and a trifluoromethyl group at the 6-position.^[9] Bromethalin is identified by CAS Registry Number 63333-35-7 and PubChem CID 44465.^[9]

Physical and chemical characteristics

Bromethalin appears as a pale white to off-white, odorless crystalline solid or powder at room temperature.^[10]^[9] It has a melting point of 150–151°C, indicating thermal stability up to moderately elevated temperatures under standard conditions.^[9]^[10] Bromethalin exhibits very low solubility in water, with a value of less than 0.01 mg/L at 20°C, which limits its mobility in aqueous environments. In contrast, it is highly soluble in various organic solvents, such as dichloromethane (300–400 g/L), chloroform (200–300 g/L), and acetone, facilitating its formulation in non-aqueous systems.^[9]^[11] The compound is generally stable under normal storage conditions and resistant to hydrolysis across pH 5-9. However, it degrades under exposure to ultraviolet light.^[11]^[9] Bromethalin has a pKa of 9.0, suggesting it exists predominantly in its neutral form under physiological conditions. Its octanol-water partition coefficient (logP) is approximately 7.68, reflecting high lipophilicity that contributes to its ability to cross the blood-brain barrier.^[11]^[10]

History

Development

Bromethalin was developed in the mid-1970s by researchers at Lilly Research Laboratories, a division of Eli Lilly and Company, as a response to the growing problem of warfarin resistance in rodent populations.^[1]^[12] The motivation stemmed from reports of anticoagulant resistance first identified in Norway rats in the 1950s and 1960s, which reduced the efficacy of multi-feed rodenticides and necessitated a single-feed alternative with a novel non-anticoagulant mechanism.^[12] This effort aimed to provide effective control against resistant strains while minimizing bait shyness in target rodents such as rats and mice.^[13] The compound emerged from a systematic screening program focused on N-alkyldiphenylamine derivatives for their potential neurotoxic effects on rodents.^[12] Key researcher Barry A. Dreikorn led the synthesis and structure-activity relationship studies, identifying bromethalin (N-methyl-2,4,6-tribromo-N-(2,4-dinitro-6-trifluoromethylphenyl)aniline) as a highly potent candidate after evaluating various substitutions for toxicity and palatability.^[14]^[12] The development process involved multi-step chemical synthesis, including coupling of substituted anilines with benzotrifluorides, followed by bromination, nitration, and N-methylation to optimize rodent-specific lethality.^[14] Initial laboratory testing confirmed bromethalin's efficacy, with acute oral LD50 values around 2.0 mg/kg in rats, demonstrating rapid onset of neurotoxic symptoms and death within days after a single feeding at concentrations of 50-100 ppm in bait.^[12] Animal studies, including non-choice and choice feeding trials on warfarin-resistant Norway rats and house mice, achieved over 90% mortality, validating its performance against resistant strains without inducing bait avoidance.^[12] These results supported patent filings, with US Patent 4,084,004 granted in 1978 and US Patent 4,187,318 issued in 1980 to Eli Lilly and Company for the rodenticidal N-alkyldiphenylamines.^[12]^[14]

Commercial introduction

Bromethalin received approval from the U.S. Environmental Protection Agency (EPA) in 1984 as a restricted-use pesticide under the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA), marking its entry into the market as a non-anticoagulant alternative for rodent control.^[15] Initially developed by Eli Lilly Research Laboratories, it was commercialized by Ralston Purina, which launched the first product under the brand name Assault in 1985.^[13]^[16] This pelleted formulation, containing 0.01% bromethalin, targeted warfarin-resistant rodents and quickly gained traction due to its single-feed efficacy.^[17] By the late 1980s, bromethalin had achieved widespread use in the United States for both urban and agricultural rodent management, particularly against Norway rats and house mice in diverse field conditions.^[17] Its adoption was driven by field trials demonstrating high efficacy, with baits placed in bait stations or broadcast applications proving effective in controlling commensal rodent populations.^[17] Over time, the product line expanded through various manufacturers, leading to continued availability under the Assault brand and similar formulations.^[15] Global expansion began in the late 1980s with introductions in Europe by Ciba-Geigy under the trade name DORATID, featuring 0.005% to 0.01% grain-based baits and weather-resistant blocks registered in countries including Switzerland, France, Denmark, and England.^[16] By the 1990s, bromethalin reached Asia and other regions, with adapted formulations to meet local regulatory and environmental needs, such as varying concentrations for field rodents.^[16] This international rollout supported its role in integrated pest management programs beyond the U.S. The compound's popularity surged in the post-1990s era, fueled by increasing resistance to anticoagulant rodenticides like warfarin and second-generation variants, positioning bromethalin as a key option for effective, resistance-independent control.^[13]^[18]

Uses

Rodent control

Bromethalin serves as a primary rodenticide for controlling commensal rodent populations, particularly targeting rats of the genus Rattus (such as Norway rats, Rattus norvegicus, and roof rats, Rattus rattus) and house mice (Mus musculus). It is especially effective against strains resistant to warfarin and other first-generation anticoagulants, addressing a key challenge in urban and agricultural pest management.^[12]^[10] The compound is formulated for single-feed application, with a typical lethal dose achieved at concentrations of 50-250 ppm in bait, often standardized at 0.01% (100 ppm) bromethalin in palatable matrices. In rats, the oral LD50 is approximately 2 mg/kg, while for mice it is approximately 5.3 mg/kg; these values underscore its high potency against target species.^[12]^[10] Key advantages of bromethalin include its rapid onset, with mortality occurring in 2-7 days post-ingestion due to its neurotoxic action disrupting cerebral edema. Unlike anticoagulants, it induces no bait shyness, as rodents do not associate the bait with illness before death, and resistance development remains low, even in populations previously exposed to other rodenticides.^[12]^[10] For effective deployment, bromethalin baits are placed in tamper-resistant stations to minimize access by non-target wildlife and domestic animals, suitable for both indoor and outdoor urban environments such as buildings, sewers, and perimeters. Stations should be positioned along rodent runways, renewed every few days to ensure consumption, and spaced according to species—typically 8-12 feet for mice and 15-50 feet for rats—to cover active areas comprehensively.^[12]^[10]^[19] Field trials demonstrate high efficacy, with bromethalin reducing rodent populations by over 90% in controlled evaluations against Norway rats and house mice under diverse conditions, including anticoagulant-resistant infestations.^[12]

Formulation and application

Bromethalin is commonly formulated as rodenticidal baits containing 0.01% active ingredient by weight, including blocks, pellets, granules, place packs, tablets, and impregnated materials designed for palatability to target rodents. As of 2025, EPA restrictions prohibit pelleted baits in consumer markets, emphasizing enclosed or block formulations in tamper-resistant stations.^[5]^[20]^[21] These baits are often mixed with attractive substances such as grains, fats, peanut butter, or molasses to encourage consumption by rats, mice, and moles.^[22] Examples include Fastrac All-Weather Blox and Bromethalin-100 Conservation Blocks, which are solid forms optimized for weather resistance and ease of deployment.^[10] Application methods emphasize secure placement to target rodents while minimizing non-target exposure, typically using tamper-resistant bait stations positioned in and around structures, burrows, or sewers.^[20]^[10] For rats, 2–12 blocks or place packs (ranging from 0.53 oz to 3 oz each) are placed at intervals of 16–328 feet; for mice, 1–5 units are used at 8–12 foot intervals, with direct insertion into active burrows for place packs.^[10] In sewer systems or hard-to-reach areas, baits may be deployed using poles, slingshots, or unmanned aerial vehicles by trained personnel, adhering to label-specified minimum amounts of 1–2 ounces per station.^[10]^[22] Safety protocols require certified applicators for professional products, while consumer products are available for general use with tamper-resistant bait stations, personal protective equipment such as gloves, long pants, and shoes, especially for loose formulations like pellets or granules that may produce dust.^[6]^[20]^[10] Product labels mandate tamper-resistant stations for most applications, warning signs at treated sites, and regular monitoring to replace consumed bait and remove remnants, with all uses limited to within 50–100 feet of buildings depending on consumer or professional status.^[20] Storage guidelines recommend keeping bromethalin products in their original containers in cool, dry areas away from food, feed, and domestic water supplies to prevent contamination and maintain stability.^[9] While specific shelf life varies by product, formulations remain effective under proper conditions, with no liquid concentrates identified for sewer applications in standard registrations.^[9]

Mechanism of action

Biochemical pathway

Bromethalin, a neurotoxic rodenticide, undergoes rapid absorption from the gastrointestinal tract following ingestion and is primarily metabolized in the liver via cytochrome P450-mediated N-demethylation to its active metabolite, desmethylbromethalin (DMB). This hepatic bioactivation process converts the parent compound into DMB, which is equipotent or more toxic than bromethalin itself and serves as the primary agent responsible for cellular toxicity. The metabolism occurs efficiently, with peak plasma concentrations of bromethalin achieved within 4 hours in rodents, facilitating quick distribution to target tissues.^[23]^[1]^[24] The core biochemical mechanism of bromethalin's toxicity involves DMB uncoupling oxidative phosphorylation in neuronal mitochondria, disrupting the electron transport chain's ability to drive ATP production. Normally, this process couples proton gradient dissipation to the phosphorylation of ADP to ATP; however, uncoupling dissipates the gradient as heat without ATP synthesis, leading to severe energy depletion in high-demand cells like neurons.

\text{ADP} + \text{P}_\text{i} + \text{nH}^+ \rightarrow \text{ATP} + \text{H}_2\text{O}

This reaction is inhibited, resulting in ATP shortages that impair ion pumps and cellular homeostasis. Bromethalin's high lipophilicity (log P ≈ 6.7) and that of DMB (log P ≈ 4.3) enable both compounds to readily cross the blood-brain barrier, leading to selective accumulation in the central nervous system where mitochondrial disruption is most pronounced. Biochemical effects begin within hours of ingestion due to rapid absorption and bioactivation, with energy depletion and related cellular stress peaking at 24-48 hours as the active metabolite concentrates in neural tissues.^[13]^[25]^[26]

Effects on the nervous system

Bromethalin's interference with neuronal energy metabolism leads to rapid depletion of adenosine triphosphate (ATP) in the central nervous system (CNS), primarily through the action of its metabolite desmethylbromethalin (DMB). This uncoupling of oxidative phosphorylation in neuronal mitochondria reduces ATP availability, impairing the function of sodium-potassium ATPase pumps essential for maintaining cellular ionic gradients.^[1]^[10] As a result, neurons experience cytotoxic edema due to sodium influx and osmotic imbalance, causing cellular swelling and disrupted neural signaling.^[27] The accumulation of fluid within myelin sheaths exacerbates these effects, resulting in intramyelinic edema and increased intracranial pressure from brain swelling. This cerebral edema compresses neural tissues, further inhibiting axonal transmission and contributing to widespread CNS dysfunction.^[1]^[10] Pathological examinations reveal characteristic spongiform degeneration and vacuolation in the white matter of the brain and spinal cord, with fluid-filled spaces forming without significant inflammation or neuronal necrosis.^[27]^[24] These disruptions manifest as a progressive syndrome of neurological impairment, beginning with hindlimb weakness and ataxia, advancing to tremors and muscle rigidity, and culminating in convulsions, paralysis, and respiratory failure as edema intensifies.^[1]^[27] The onset and severity vary by species, with smaller mammals like rats and cats exhibiting faster progression due to their higher metabolic rates and greater sensitivity to ATP depletion compared to larger species such as dogs.^[10]^[28]

Toxicology

Acute toxicity in target species

Bromethalin demonstrates high acute toxicity in target rodent species, primarily rats and mice, with oral LD50 values reported as 2.11 mg/kg for male rats, 3.17 mg/kg for female rats, and 5.3 mg/kg for house mice.^[10] These values indicate that a single ingestion of a formulated bait containing approximately 0.01% bromethalin can deliver a lethal dose to most individuals, reflecting its design as a single-feed rodenticide.^[10] At doses equivalent to the LD50, death occurs within 1-3 days in rodents, primarily due to neurotoxic effects leading to respiratory arrest, though higher doses exceeding the LD50 can accelerate onset to 8-12 hours with preceding clonic convulsions.^[27] Sublethal exposures result in hind-limb weakness, paralysis, and loss of tactile sensation, potentially impairing mobility and survival without causing immediate fatality.^[27] Clinical signs in affected rodents progress from initial lethargy and ataxia to more severe manifestations such as tremors and convulsions, culminating in coma and death from respiratory failure.^[27] These symptoms arise from the compound's disruption of neuronal function, though detailed biochemical pathways are addressed elsewhere. Bromethalin's resistance profile remains robust, with low documented incidence in target populations owing to its acute, single-feeding mode of action that limits survival and reproduction of exposed individuals; no cross-resistance with anticoagulant rodenticides has been observed, as its mechanism differs fundamentally.^[10] Post-mortem findings in poisoned rodents consistently include cerebral edema and spongy degeneration of the white matter in the brain and spinal cord, with intramyelinic edema visible on ultramicroscopic examination but without associated inflammation or neuronal destruction.^[27]

Toxicity to non-target organisms

Bromethalin presents significant risks to non-target organisms through secondary poisoning, as predators and scavengers ingest rodents containing residues of the compound and its toxic metabolite, desmethylbromethalin. This metabolite accumulates in the fatty tissues of poisoned prey, facilitating transfer up the food chain to species such as owls, hawks, foxes, and bobcats. Due to bromethalin's lipophilic properties, residues can persist and concentrate in predators, increasing the potential for biomagnification, though exact factors in terrestrial ecosystems remain understudied.^[29]^[30] Avian species are highly susceptible to bromethalin toxicity, with acute oral LD50 values indicating variable sensitivity across taxa; for example, bobwhite quail exhibit an LD50 of 4.56 mg/kg, while mallard ducks have a dietary LC50 of 620 mg/kg. Raptors appear particularly vulnerable, with tolerances similar to small mammals, and exposure often manifests as neurological impairment including ataxia, tremors, and hindlimb paralysis, progressing to death from cerebral edema within hours to days. A 2023 study detected bromethalin exposure in approximately 30% of sampled birds of prey, underscoring the compound's role in wildlife morbidity.^[31]^[10]^[32] Non-target mammals, including domestic and wild species, face comparable risks to target rodents, given similar acute toxicity profiles; dogs, for instance, have an oral LD50 of approximately 4 mg/kg (range 2.4–5.6 mg/kg), similar to the 2.11–3.17 mg/kg reported for rats. A 2025 retrospective study confirmed the oral LD50 in dogs as 2.4–5.6 mg/kg.^[31]^[10]^[18] Chronic sublethal exposures at levels exceeding the no-observed-adverse-effect level (NOAEL) of 0.025 mg/kg/day, such as the LOAEL of 0.125 mg/kg/day, may cause neurological effects like tremors and ataxia, potentially impairing behavior in affected populations.^[31]^[10] Ecological assessments reveal broader impacts on predator communities, with 52 documented wildlife poisoning incidents involving bromethalin between 2010 and 2018, including raptors such as great horned owls and red-tailed hawks. These exposures contribute to elevated mortality in localized raptor populations, exacerbating declines from cumulative rodenticide effects. The persistence of desmethylbromethalin in tissues, with a half-life of approximately 5.6 days in mammalian models but detectable for weeks in adipose, prolongs secondary exposure risks and amplifies ecological disruptions.^[30]^[29]

Poisoning in humans and animals

Symptoms and diagnosis

Bromethalin poisoning in humans typically begins with initial gastrointestinal symptoms such as nausea, vomiting, abdominal pain, and diarrhea, often occurring within 2-24 hours of exposure.^[3] These may progress to neurological manifestations including headache, confusion, agitation, lethargy, muscle weakness, tremors, ataxia, seizures, coma, and respiratory depression, particularly in cases of higher doses or intentional ingestion.^[33]^[34] Accidental exposures in children are usually mild and self-limited, resolving without severe outcomes, but severe cases can lead to cerebral edema and increased intracranial pressure.^[35] In dogs and cats, symptoms vary by dose and species but generally involve central nervous system effects due to brain edema. High doses in dogs (>3.5 mg/kg) cause an acute convulsant syndrome within 4-36 hours, featuring hyperexcitability, muscle tremors, hyperthermia, grand mal seizures, and hindlimb hyperreflexia, often progressing to death.^[1] Lower doses lead to a delayed paralytic syndrome in 1-5 days, with ataxia, hindlimb paresis or paralysis, mild tremors, and possible seizures.^[36] Cats exhibit primarily paralytic signs regardless of dose, including depression, anorexia, vomiting, ataxia, decreased intestinal motility, pelvic limb weakness progressing to paralysis, tremors, seizures, and coma, with onset in 1-7 days.^[37] Symptoms can be exacerbated by stimuli like light or noise.^[38] Diagnosis of bromethalin poisoning relies on a history of exposure to the rodenticide, combined with compatible clinical signs appearing within hours to days.^[1] Confirmatory testing involves detection of bromethalin or its active metabolite, desmethylbromethalin, in serum, fat, liver, kidney, or brain tissue using gas chromatography or high-performance liquid chromatography, though such assays are available only at specialized toxicology labs and often postmortem.^[3] Serum chemistry may show nonspecific changes, including elevated creatine kinase from muscle activity during tremors or seizures and hyperglycemia from stress response; baseline blood glucose and electrolyte panels are recommended.^[1] Magnetic resonance imaging (MRI) can reveal brain edema, such as T2 hyperintensities in white matter, supporting the diagnosis antemortem.^[28] Differential diagnosis includes other neurotoxins and must rule out anticoagulant rodenticides via prothrombin time and partial thromboplastin time tests, and organophosphates or carbamates via serum or red blood cell cholinesterase activity assays.^[3] Additional considerations encompass lead toxicity, ethylene glycol, strychnine, metaldehyde, zinc phosphide, tremorgenic mycotoxins, and other causes of seizures like trauma or infectious diseases.^[1] Prognosis is poor once neurological symptoms develop, with high fatality rates in severe cases, often requiring euthanasia or resulting in death despite intervention.^[36]^[37] Milder exposures may allow recovery over weeks, though permanent neurological deficits can persist.^[1] In humans, unintentional low-dose exposures are rarely fatal, but severe ingestions carry grave risk.^[35]

Treatment and management

Treatment of bromethalin poisoning lacks a specific antidote, unlike anticoagulant rodenticides that respond to vitamin K, necessitating aggressive decontamination to prevent absorption and comprehensive supportive care to manage symptoms such as seizures and cerebral edema.^[1]^[39] Decontamination protocols prioritize early intervention; in asymptomatic animals, emesis is induced within 2-4 hours post-ingestion using agents like apomorphine (dogs) or hydrogen peroxide (dogs), followed by multiple doses of activated charcoal (1-3 g/kg orally every 6-8 hours for 1-3 days) to adsorb the toxin and counter its enterohepatic recirculation.^[40]^[1] In humans, activated charcoal is administered promptly to inhibit absorption, with gastric lavage considered under poison control guidance if ingestion is recent and large.^[39]^[25] For clinically affected patients, IV fluid therapy supports hydration and diuresis, while monitoring for electrolyte imbalances and aspiration risk is essential during charcoal administration.^[1] Supportive care focuses on neurological stabilization; anticonvulsants such as diazepam or benzodiazepines are used to control seizures in both animals and humans, with mannitol (0.25-0.5 g/kg IV over 20-30 minutes, repeatable every 4-8 hours) or hypertonic saline employed to reduce intracranial pressure from cerebral edema in hydrated patients.^[40]^[39]^[1] Hospitalization for at least 24-48 hours in animals and 12 hours or more in humans with suspected significant exposure allows for continuous monitoring of neurological status, vital signs, and intracranial pressure, with prognosis improving markedly if intervention occurs before severe symptoms develop.^[40]^[39] In veterinary cases, particularly for dogs, intravenous lipid emulsion therapy (e.g., 1.5 mL/kg 20% lipid solution as a bolus followed by infusion) is an experimental adjunct for severe toxicity due to bromethalin's lipophilicity, potentially reducing serum toxin levels by up to 75% in reported cases, though it is reserved for non-responders to standard therapies and requires careful monitoring for complications.^[41] Human guidelines emphasize consultation with poison control centers for tailored management, including potential use of lipid emulsion in life-threatening neurological cases, underscoring the need for rapid decontamination and symptom control to mitigate irreversible brain damage.^[39]^[25]

Regulatory status

Approval and restrictions

Bromethalin was first registered by the United States Environmental Protection Agency (EPA) in 1984 as a rodenticide and is classified as a restricted-use pesticide (RUP) for professional and agricultural applications, necessitating use only by certified applicators who have completed mandatory training programs.^[42]^[5] Consumer products containing bromethalin are limited to smaller packaging sizes, typically one pound or less, and are prohibited from sale in general retail outlets like grocery stores, further emphasizing its controlled status to minimize accidental exposures.^[5] Internationally, bromethalin is not approved for use in the European Union under Regulation (EC) No 1107/2009, resulting in its effective ban across EU member states due to concerns over health and environmental risks.^[7] In the United States, regional variations exist, such as in California, where its application is restricted solely to the control of rats and mice, with state-specific formulations required to comply with enhanced regulatory standards for structural pest management.^[43] All bromethalin products must adhere to stringent labeling requirements, including the mandatory use of tamper-resistant bait stations designed to withstand tampering by children under six years of age and domestic animals, as well as child-resistant packaging for formulations accessible to the public.^[5] Labels are required to prominently feature warnings about the dangers to pets and non-target wildlife, along with instructions for secure placement and disposal to prevent secondary poisoning.^[44] As of 2025, the EPA has opened the docket for bromethalin's registration review, assessing its ongoing safety profile in light of evolving alternatives to second-generation anticoagulant rodenticides, which face increasing limitations; while no nationwide bans are in place, urban use restrictions have been tightened to reduce risks in populated areas.^[45]^[5] Bromethalin's international trade is governed by general protocols for hazardous pesticides, though it is not included in Annex III of the Rotterdam Convention and thus exempt from the convention's prior informed consent procedure for exports to participating nations.^[46]

Environmental considerations

Bromethalin exhibits low water solubility, <0.01 mg/L at 25°C, which restricts its mobility in aqueous environments and limits potential runoff into surface waters during typical use scenarios.^[9] In soil, it demonstrates high persistence with an aerobic half-life ranging from 132 to 235 days, depending on environmental conditions, rendering it hardly mobile due to a strong affinity for organic matter (Koc ≈ 55,000 L/kg).^[30] The primary degradation product is desnitrobromethalin, which can accumulate to up to 64% of the parent compound over time, though its ecological toxicity remains incompletely characterized.^[30] Due to its lipophilic nature (log Kow ≈ 7.6), bromethalin shows significant potential for bioaccumulation in fatty tissues of organisms, with a measured bioconcentration factor (BCF) of 120,000 in fish whole-body tissues under laboratory conditions.^[30] This affinity raises concerns for secondary poisoning risks within food chains, particularly for predators consuming contaminated prey shortly after bait exposure, although rapid elimination (plasma half-life of about 5.6 days) may mitigate long-term residue buildup in some species.^[30] Ecological impact studies indicate bromethalin poses moderate to high risks to non-target wildlife, especially birds of prey, with U.S. Environmental Protection Agency (EPA) risk quotients (RQs) for species like the great horned owl and barn owl ranging from 2.4 to 20, exceeding the agency's level of concern (LOC) of 0.5.^[30] Wildlife incident reports from 1996 to 2018 document 56 cases involving non-target species, with over 90% occurring after 2010 and a notable proportion in urban areas such as California and New York, where rodenticide use is intensive.^[30] Recent field studies, including a 2023 analysis of adipose tissues from 44 birds of prey in Massachusetts, detected bromethalin residues in approximately 30% of samples across species like red-tailed hawks and barred owls, suggesting ongoing exposure that may contribute to population-level stresses in raptors.^[29] To mitigate these risks, the use of tamper-resistant bait stations is mandated for above-ground applications, effectively reducing accidental exposure to non-target wildlife by containing baits and limiting access.^[30] Integrated pest management (IPM) approaches are recommended, emphasizing non-chemical methods such as sanitation, exclusion, and monitoring alongside targeted baiting to minimize overall environmental release and ecological disruption.^[42] Post-2020 wildlife studies highlighting bromethalin exposure in avian species have prompted increased monitoring efforts, with calls for enhanced surveillance in urban ecosystems through 2025 to better assess persistence and long-term effects, including potential accumulation in sediments where data gaps persist.^[29]^[32]

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May 2, 2018 · Bromethalin is rapidly absorbed after ingestion and metabolized via N-demethylation in the liver to desmethylbromethalin (DMB) in most species, ...
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Rodenticide Toxicity - StatPearls - NCBI Bookshelf
Jun 22, 2024 · The clinical presentation of rodenticide toxicity varies widely. Common symptoms range from anticoagulant-induced bleeding to neurological and metabolic ...Missing: adoption timeline
[25]
Bromethalin poisoning in a raccoon (Procyon lotor)
Dec 9, 2013 · ... Bromethalin—a promising new rodenticide. In: Proceedings of the Tenth Vertebrate Pest Conference, ed. Marsh RE, pp. 10–16, Monterey, CA). To ...
[26]
The toxicity and mechanism of action of bromethalin: a new single ...
Bromethalin is a new rodenticide for the control of commensal rodents. Doses in excess of the LD50 (2 mg/kg in rats) will cause death within 8-12 hr.Missing: development history
[27]
MRI, Clinical, and Pathological Correlates of Bromethalin Toxicosis ...
Apr 26, 2022 · Bromethalin toxicosis is an increasingly common clinical presentation in dogs that may be fatal depending on the extent of intoxication.
[28]
Active metabolite of the neurotoxic rodenticide bromethalin along ...
Sep 15, 2023 · Bromethalin and anticoagulant rodenticides were studied in birds of prey. •. Bromethalin's active metabolite, desmethylbromethalin, was found in ...
[29]
[PDF] 2020.03.31 08:41:46 -04'00' ELIZABETH DONOVAN
Bromethalin can be characterized as persistent in soil based on the Goring (1975) scale, hardly mobile. (FAO scale) based on an organic carbon partition ...
[30]
Mechanisms of toxicity and residue considerations of rodenticide ...
Jan 28, 2022 · Clinical signs are dose-dependent and appear between 4 hours and 7 days after ingestion. Methods have been recently published for the ...<|control11|><|separator|>
[31]
New Study is First to Find Exposure to Neurotoxic Rodenticide ...
Jul 11, 2023 · They found evidence of bromethalin exposure in about 30% of the birds of prey that were sampled. How Bromethalin Works. In 2008, the U.S. ...
[32]
[PDF] The EPA Rodenticide Ban and Bromethalin - Maryland Poison Center
Aug 18, 2014 · Bromethalin is a rodenticide that has replaced LAA's in many rat and mouse poison products sold to the public. It is a potent neurotoxin, with ...
[33]
Fatal bromethalin poisoning - PubMed
Bromethalin is a neurotoxin found in some rodenticides. A delusional 21-year-old male presented to a hospital with altered mental status the day after ...
[34]
Human bromethalin exposures reported to a U.S. Statewide Poison ...
Adverse effects were minor, self-limited, and mostly gastrointestinal upset. There were no moderate, major, or fatal effects in our study population. The ...
[35]
Bromethalin Rodenticide Poisonings in Dogs - VCA Animal Hospitals
Bromethalin is a common rodenticide that targets the central nervous system, causing delayed paralytic syndrome with lethargy, or acute convulsant syndrome ...
[36]
Bromethalin Rodenticide Poisoning in Cats - VCA Animal Hospitals
Cats are more sensitive to bromethalin and develop toxicity at lower doses than dogs. Also, unlike dogs, cats develop common signs at any toxic dose.Missing: stability | Show results with:stability
[37]
Poison Alert: Beware of Bromethalin - ASPCA
May 30, 2019 · Bromethalin is a neurotoxin that can be very harmful to your pet. Rat poisons often contain peanut butter and/or sugar to attract rodents.Missing: timeline | Show results with:timeline
[38]
None
### Bromethalin Treatment in Humans
[39]
Treating Bromethalin Toxicosis - ASPCApro
There is no effective antidote for bromethalin. Emesis and activated charcoal are used to prevent toxicity. Lipid emulsion therapy's efficacy is unknown.
[40]
Intravenous Lipid Emulsion Therapy for Bromethalin Toxicity in a Dog
Jul 1, 2016 · Use of activated charcoal has been recommended with bromethalin ingestion, as it has significant enterohepatic recirculation. ... However, the ...
[41]
Rodenticides - National Pesticide Information Center
Bromethalin is considered a single-dose rodenticide. Cholecalciferol was first registered as a rodenticide in the United States in 1984. Cholecalciferol is ...
[42]
EU Pesticides Database - Active substances - European Commission
Active substance: Bromethalin. Status under Reg. (EC) No 1107/2009Not approved. (repealing Directive 91/414/EEC). Legislation, 2004/129/EC, Old legislation.
[43]
Rodenticides: Further Restrictions in 2025 | Pests in the Urban ...
Jul 14, 2025 · Toxic baits, or rodenticides, used to control rodents around the home and garden can harm nontarget animals, such as pets, birds, coyotes, ...Missing: target organisms
[44]
[PDF] CA Notice 2025-05 - California Department of Pesticide Regulation
Apr 3, 2025 · Second-generation anticoagulant rodenticide (SGAR) products are those that contain the active ingredients brodifacoum, bromadiolone ...
[45]
Child-Resistant Packaging for Pesticides | US EPA
Sep 22, 2025 · Does Your Pesticide Product Require Child-Resistant Packaging? · Acute oral LD50 of 1.5 g/kg or less. · Acute dermal LD50 of 2000 mg/kg or less.Missing: Bromethalin | Show results with:Bromethalin
[46]
[PDF] Scanned Document - Regulations.gov
Table 3: Bromethalin Use and Usage Information. Summary ofUse. Bromethalin is a non-anticoagulant rodenticide that is used in bait products to control ...
[47]
Annex III Chemicals - Rotterdam Convention
The chemicals listed in Annex III include pesticides and industrial chemicals that have been banned or severely restricted for health or environmental reasons.Missing: Bromethalin | Show results with:Bromethalin