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Must

Must is the unfermented juice extracted from freshly crushed grapes, typically including the skins, seeds, and sometimes stems, which forms the foundational material in the winemaking process.^[1] This mixture, known as pomace when referring to the solid components, originates from the Latin term vinum mustum, meaning "young wine," and is essential for initiating fermentation.^[2] The composition of must varies based on grape variety, ripeness, climate, and soil conditions, but it generally consists of water, sugars (ranging from 170–220 g/L), organic acids (9–27 g/L), nitrogen compounds (0.1–1 g/L), minerals, polyphenols, vitamins, and aromatic precursors that influence the final wine's flavor, color, and stability.^[3] In red winemaking, the inclusion of skins and seeds imparts tannins and color during maceration, while white wines often use clarified must from free-run juice or gentle pressing to avoid excessive bitterness.^[2] Must weight, measured by scales like Brix (typically 21–25° for table wines), indicates potential alcohol content, as each degree Brix roughly converts to 0.55% alcohol upon full fermentation.^[1] During winemaking, must undergoes alcoholic fermentation driven by yeast such as Saccharomyces cerevisiae, which consumes sugars and produces ethanol, carbon dioxide, and heat; the amino acids and nitrogen in must serve as critical nutrients for this process.^[3] Insufficient nitrogen can hinder fermentation, sometimes requiring additions like diammonium phosphate (up to 96 g/hL in the United States).^[3] Beyond grapes, must can derive from other fruits like apples or berries for cider or fruit wines, though grape must remains central to viticulture worldwide.^[2]

Etymology and definition

Etymology

The term "must" in the context of winemaking derives from the Latin mustum, literally meaning "new" or "young wine," a neuter form of mustus denoting something fresh or undiluted, possibly linked to a Proto-Indo-European root meus- implying dampness or wetness.^[4] This usage appears in ancient Roman texts, with one of the earliest detailed references found in Pliny the Elder's Naturalis Historia (77 CE), where he describes various types of must, including semper mustum (ever-fresh must), a non-fermenting grape juice preserved for sweetness.^[5] The word's application to unfermented grape juice reflects Roman viticultural practices, where mustum was the starting point for wine production. In the evolution of English, the noun "must" entered the language in the Old English period, derived from Latin mustum.^[4] Earlier attestations in Old English exist for the modal verb sense of obligation, but the winemaking meaning specifically denotes unfermented fruit juice. This linguistic path parallels developments in other Romance languages, such as Italian mosto and Spanish mosto, both direct descendants of mustum and retaining the core idea of fresh, pressed grape liquid. Related terms appear in earlier Indo-European languages, including ancient Greek oinos gleukos, translating to "sweet wine" or new, unfermented grape juice, used in classical texts to describe a similar fresh product.^[6] Over time, the terminology shifted from these classical roots to modern oenological usage, where "must" consistently refers to the pre-fermentation stage in fruit processing, maintaining its association with novelty and freshness across centuries of viticultural literature.^[4]

Definition

Must is the freshly pressed liquid obtained from fruits, primarily grapes, that includes the juice along with pulp, skins, seeds, and sometimes stems. The solid components, collectively known as pomace, typically comprise 10–20% of the must's total weight. This unfermented mixture serves as the starting material in various beverage productions, retaining the solids to facilitate the extraction of flavors, colors, and other compounds during subsequent processes.^[7]^[8] Unlike clarified fruit juice, from which solids have been separated, must preserves these elements for their contributory role in the final product; it is distinct from fermented beverages such as wine or distilled spirits like brandy. The term derives from the Latin mustum, meaning "young wine" or "new wine." According to the International Organisation of Vine and Wine (OIV), grape must is specifically the liquid product resulting from fresh grapes through physical means like crushing or maceration.^[8] In winemaking, must is categorized by the extent of skin contact: white must is produced by pressing grapes immediately to minimize color and tannin extraction from the skins; red must involves fermentation in the presence of skins to impart color via anthocyanins and structure from tannins; rosé must receives limited skin contact, often just a few hours, to achieve a pale pink hue. Legally, under European Union regulations aligned with OIV standards, grape must is recognized as an unfermented product from fresh grapes, and in Protected Designation of Origin (PDO) specifications—such as for Traditional Balsamic Vinegar of Modena or Reggio Emilia—it must derive from designated grape varieties with controlled yields ensuring adequate sugar content for concentration and aging.^[8]

Production

Grape must production

Grape must production begins with harvesting, where timing is determined by grape ripeness, typically measured using the Brix scale to assess sugar content. For most still wines, grapes are harvested at 21-25° Brix to achieve optimal balance of sugars, acids, and flavors, while sparkling wine grapes are picked earlier at 18-20° Brix. Harvesting methods vary by scale and terrain: hand-picking is preferred in steep vineyards or for premium quality to select only ripe clusters and avoid damage, whereas mechanical harvesters are used in flatter, large-scale operations for efficiency, though they may include unripe berries or MOG (material other than grapes).^[9] Following harvest, the grapes undergo crushing and destemming to release the juice while minimizing seed breakage, which could introduce bitter tannins. Modern crushers-destemmers employ rubber rollers or paddles to gently break the berry skins, producing a must consisting of free-run juice, pulp, skins, and seeds, with typical juice yields of 70-80% by weight depending on grape variety and processing. This step is crucial for white wines, where destemmed grapes may be pressed immediately to limit skin contact and oxidation, unlike reds where whole clusters might be included for flavor enhancement.^[10]^[11] Pressing extracts additional juice from the crushed grapes, with techniques varying by wine type to preserve quality. For white wines, gentle pneumatic bladder presses apply controlled air pressure to separate juice without excessive phenolics or oxidation, often yielding clearer must suitable for early fermentation. In contrast, traditional basket or vertical screw presses are favored for reds post-maceration, as their slower, firmer action extracts colored juice while allowing some tannin integration, though modern producers may use pneumatic presses for both to standardize efficiency.^[12]^[13] Contemporary practices incorporate temperature control and additives to stabilize the must before fermentation. Grapes are often chilled during transport and processing to 10-15°C, inhibiting indigenous yeasts and enzymes that could cause premature fermentation or spoilage. Sulfur dioxide (SO₂) is routinely added at 25-75 mg/L to the must for antimicrobial action and to prevent enzymatic browning, particularly in whites, ensuring microbial stability without altering flavor profiles.^[14]^[15] Yield factors significantly influence must volume and quality, varying by grape variety and regional traditions. Thin-skinned varieties generally produce higher juice yields than thick-skinned ones due to a lower skin-to-pulp ratio, affecting overall efficiency.^[16] Regional practices, such as foot-treading in Portugal's Douro Valley for Port wine, involve treading grapes in shallow stone lagares to gently crush berries and initiate color extraction, a method retained for its ability to enhance tannin structure over mechanical alternatives.^[17]

Must from other fruits

Must from fruits other than grapes is produced through adapted pressing and extraction techniques that account for variations in fruit texture, sugar content, and composition. For apple must used in cider production, apples are first milled into a pomace—a pulpy mash—that facilitates juice release during pressing.^[18] Hydraulic presses are commonly employed to apply controlled pressure, extracting 65-75% juice by weight from the pomace, depending on apple variety and ripeness.^[19] Specific cider apple varieties, such as bittersweets like Dabinett, are selected for their balanced tannins and acidity, contributing to the must's structure without overpowering astringency.^[20] Pear must for perry follows a similar process to apple must but requires adjustments due to the fruit's softer texture. Pears are milled into pomace and pressed, often using hydraulic or basket presses, though gentler pressure is applied to prevent excessive extraction of tannins that could impart unwanted bitterness.^[21] Perry pears, like Huffcap or Barland, are typically harvested late and allowed to soften slightly before processing to optimize yield while minimizing astringency.^[22] For other fruits, extraction methods vary to suit their properties. Berry must, such as from blackcurrants used in liqueurs, is often produced via steam juicing, where heat vaporizes water from the berries to collect condensed juice, avoiding direct crushing that could oxidize delicate flavors.^[23] Tropical fruits like pineapple and mango, which have high fiber and enzyme content, undergo enzymatic treatment with pectinases and cellulases to break down cell walls and facilitate juice release, improving yield and clarity.^[24] Producing must from non-grape fruits presents challenges, including higher pectin levels in many varieties that cause cloudiness and viscosity, necessitating fining agents like pectinase enzymes to clarify the juice.^[25] Additionally, some fruits have lower natural sugars, requiring concentration techniques such as evaporation or membrane filtration to achieve suitable levels for fermentation.^[26] Historically, must from fruits like mulberries and quinces was utilized in medieval Europe for beverages and preserves, with records indicating mulberry-based wines and quince conserves in monastic and noble households.^[27]

Chemical composition

Main components

Must, the freshly crushed grape juice including skins, seeds, and pulp, contains several key chemical components that influence its fermentation and the resulting wine quality. The primary soluble solids are sugars, predominantly glucose and fructose, which typically constitute 10-25% of the must by weight, equivalent to 100-250 g/L.^[28] These sugars are measured using scales such as Brix (degrees Brix, where 1°Brix approximates 1% sugar) or Oechsle, providing an estimate of ripeness and potential alcohol yield.^[29] During fermentation, yeasts convert these sugars to ethanol, with the theoretical potential alcohol by volume (ABV) calculated as sugar content (in °Brix) multiplied by approximately 0.55, though actual yields vary due to fermentation efficiency.^[30] Organic acids form another major component, accounting for 0.5-1.2% of the must (5-12 g/L total acidity), primarily tartaric and malic acids (comprising about 90% of the total), with smaller amounts of citric acid; ranges vary by climate and ripeness.^[31] These acids contribute to a pH range of 3.0-4.0 in typical grape must, which is crucial for microbial stability by inhibiting the growth of spoilage bacteria and unwanted yeasts.^[32] Suspended solids, known as pomace, make up 7-23% of the must weight, depending on the extent of crushing and grape variety, and include skins, seeds, and pulp.^[33] These solids are rich in phenolics and tannins extracted from the skins and seeds, which provide structure, color, and astringency to the wine, as well as pectins from the pulp that can affect clarity if not managed.^[7] Additional minor components include proteins and enzymes at 0.1-0.5 g/L (0.01-0.05%), which can influence haze formation and enzymatic reactions during processing.^[34] Trace minerals such as potassium (around 1,000 mg/L) and nitrogen compounds (yeast assimilable nitrogen, YAN, at 60-500 mg/L) support yeast nutrition and fermentation vigor.^[35] Aroma precursors, including terpenes (e.g., linalool for floral notes) and cysteine-bound thiols (precursors to tropical fruit aromas like 3-mercaptohexanol), are present in trace amounts and are released during fermentation to define varietal character.^[36] The composition of must varies significantly; late-harvest grapes yield higher sugar levels (up to 30% or more), increasing alcohol potential, while cool-climate regions produce must with elevated acidity (higher tartaric and malic content) for better balance and preservation.^[37]

Physical properties

Must is a cloudy liquid obtained from crushed grapes, characterized by suspended solids including pulp, skins, and seeds that contribute to its opacity and texture. For white grape varieties, the must appears as a pale yellow liquid, while red grape must, often including macerated skins, takes on a deep purple hue. Turbidity levels, arising from these suspended solids, can range from hundreds to several thousand NTU in raw must, with white must typically up to around 1,000 NTU and red must up to 5,000 NTU or more, influencing clarity and processing decisions.^[38]^[39]^[40] The density of must, also known as specific gravity, typically ranges from 1.08 to 1.12 g/cm³, largely due to dissolved sugars that increase its mass relative to water. This property is routinely measured with a hydrometer to assess must weight, providing an indicator of sugar concentration and potential alcohol yield after fermentation.^[41]^[42] Viscosity in must exceeds that of water, generally falling between 3 and 5 cP at ambient temperatures (around 20°C), owing to the presence of pectins and sugars that thicken the liquid; unclarified must with higher solids content may be more viscous. This elevated viscosity affects practical handling, such as pumping through equipment and the rate of settling during clarification.^[43] Must exhibits temperature sensitivity critical to quality preservation, with optimal handling and storage occurring between 10 and 20°C to limit microbial activity and oxidative changes. Temperatures above 25°C heighten the risk of enzymatic browning, mediated by polyphenol oxidase, which oxidizes phenolic compounds and results in undesirable discoloration.^[44]^[45]^[3] The sensory profile of must includes a prominent aroma of fruity and grapey notes, primarily from naturally occurring esters that evoke fresh grape characteristics. Uncontrolled wild yeasts, however, may introduce off-flavors if not managed promptly during processing. These physical traits are shaped by underlying chemical components like sugars and organic acids.^[46]^[47]

Role in winemaking

Fermentation process

The fermentation process transforms grape must into wine primarily through alcoholic fermentation, where yeasts convert the sugars present in the must—typically 22–26° Brix—into ethanol and carbon dioxide.^[48] This biochemical reaction is initiated by the addition of yeast, most commonly Saccharomyces cerevisiae, which is either inoculated commercially at concentrations of 10^6–10^7 cells per milliliter for controlled and reliable fermentation or relies on native yeasts naturally occurring on grape skins for a more spontaneous process that can introduce varietal flavors but risks inconsistency.^[49]^[50] The fermentation begins once yeast is active, with the must's anaerobic environment favoring the glycolytic pathway that yields approximately 1% alcohol per 1.8° Brix of sugar consumed.^[51] Alcoholic fermentation proceeds in stages, starting with the primary phase, which is vigorous and lasts 5–10 days, during which the bulk of sugar conversion occurs at temperatures of 18–28°C for red wines to optimize extraction and flavor development.^[52] This phase generates significant heat and foam from CO₂ release, requiring temperature control to prevent off-flavors from excessive warmth. Following primary fermentation, a secondary phase may occur, often involving malolactic fermentation conducted by lactic acid bacteria such as Oenococcus oeni, which converts sharper malic acid to softer lactic acid, reducing acidity and enhancing mouthfeel, typically at cooler temperatures of 18–20°C over 2–8 weeks.^[53]^[54] For white wines, must is typically clarified by settling or gentle pressing to separate the liquid from the pomace early, minimizing contact with skins and seeds to avoid excessive tannins and bitterness, with fermentation proceeding at similar temperatures but without extended maceration.^[55] For red wines, must management is crucial during fermentation to maximize color and tannin extraction from the skins. The floating cap of skins, seeds, and pulp—known as the pomace—is regularly submerged through punching down, typically twice daily, or pumped over to ensure even contact with the fermenting liquid, promoting anthocyanin release while preventing the cap from drying out and harboring spoilage microbes.^[56] After 7–21 days of maceration, depending on desired extraction levels, the wine is racked off the pomace to separate the liquid from the solids, marking the end of primary skin contact.^[57] Fermentation progress is closely monitored by tracking the decline in Brix, from an initial 22° to approximately -2° for dry wines, indicating near-complete sugar depletion, alongside daily measurements of pH, temperature, and alcohol levels using hydrometers and refractometers. To avert stuck or sluggish fermentations—where yeast activity halts prematurely due to nutrient deficiencies or stress—additions of sulfur dioxide (SO₂) at 30–50 mg/L provide antimicrobial protection without inhibiting yeast, while yeast assimilable nitrogen (YAN) nutrients, often 150–250 mg/L, support robust metabolism.^[58]^[59] Successful completion yields wine with 11–15% alcohol by volume (ABV), varying with initial sugar content and yeast strain efficiency, alongside the formation of lees—sediment primarily composed of dead yeast cells and cellular debris that settle at the bottom, influencing subsequent clarification steps.^[60]^[61] This process not only preserves the must's inherent qualities but also shapes the wine's sensory profile through controlled microbial activity.

Pomace utilization

Grape pomace, the solid residue remaining after pressing or fermentation of must, primarily consists of grape skins, seeds, and pulp. It is rich in tannins and dietary fibers, with seeds contributing up to 35-40% fibers and 7-20% lipids on a dry weight basis, while the overall pomace contains phenolic compounds such as procyanidins and anthocyanins. Additionally, pomace retains 5-15% residual sugars on a dry basis, depending on the processing stage, which can support further microbial activity if not managed promptly.^[62]^[63]^[64] A primary industrial application of pomace is distillation to produce pomace brandies, where the residue undergoes fermentation of its residual sugars before direct distillation without water addition to concentrate alcohol and flavors. In Italy, this yields grappa, a clear spirit traditionally made from fermented pomace in copper pot stills, capturing volatile aromas from the skins and seeds. Similarly, in France, marc is produced through double distillation of fermented pomace, resulting in a robust brandy used in regional cuisines and aperitifs. These processes valorize what would otherwise be waste, with fermentation enhancing ester and higher alcohol profiles critical to the spirits' quality.^[65]^[66] Beyond distillation, pomace serves as animal feed, particularly for ruminants and poultry, due to its fiber and protein content, which can replace up to 20-30% of conventional grains while providing antioxidants that improve gut health and reduce emissions in dairy cattle by 10-11%.^[67]^[68]^[69] As a fertilizer or compost additive, its potassium levels—up to 1,400 mg/100 g—enrich soil nutrients, with a carbon-to-nitrogen ratio of 17:1 to 30:1 making it suitable for aerobic composting in vineyards to enhance microbial activity and structure.^[64] Pomace also supports biogas production through anaerobic digestion, yielding 250-850 mL of biogas per gram of volatile solids, with methane comprising 55-65%, offering a renewable energy source from winery waste.^[70] Recycling pomace through these uses significantly reduces winery waste, which can account for 20-30% of processed grape mass, minimizing landfill disposal and environmental impact. The European Union, via the Waste Framework Directive (2008/98/EC) and Regulation (EC) No 479/2008, mandates sustainable management of organic residues like pomace, encouraging alternatives to distillation such as composting or energy recovery to promote circular economy practices in vineyards.^[71]^[72]^[73]

Other beverages

In mead production

In mead production, the base consists of honey diluted in water to form a fermentable wash analogous to must, with fruit must often incorporated to create flavored variants known as melomels. Fruit must, derived from grapes, apples, or other fruits, is typically added at ratios of approximately 1 part fruit by weight to 1.15 parts honey, or equal volumes when using fruit juice, providing natural sugars that contribute to alcohol content and complexity while diluting the high gravity of the honey wash.^[74] This addition is essential for melomels, where the fruit must supplies acidity and tannins lacking in pure honey, balancing the beverage's sweetness and enhancing structural mouthfeel.^[74] The fermentation process mirrors winemaking but emphasizes lower temperatures of 15–21°C to preserve delicate honey aromas and flavors, with yeast such as Lalvin 71B pitched into the combined must after aeration and nutrient supplementation to manage high gravities (often 1.090–1.130).^[75] Pectic enzyme is commonly added to clarify the must and prevent haze from fruit pectin, followed by primary fermentation lasting 10–14 days, then secondary aging for several weeks to integrate flavors.^[74] Historical medieval European recipes, such as those from Russia, blended honey with fruit juice at proportions of about two-thirds honey to one-third juice, simmering the mixture before fermentation to extract flavors and stabilize the must.^[76] Modern craft meadmakers adapt these traditions, employing specific fruits like berries or stone fruits in melomels to achieve nuanced profiles. Key variations include pyment, which combines honey as the primary fermentable with grape must or juice for a wine-like character, and cyser, where apple must forms the base augmented by 1.75–2.5 pounds of honey per gallon to add body and residual sweetness.^[75]^[77] A primary challenge arises from honey's inherent antimicrobial properties—stemming from high osmolarity, low pH (3.2–4.5), hydrogen peroxide, and phenolic compounds—which can delay fermentation starts or cause "stuck" batches when diluted by fruit must, necessitating careful yeast nutrition and monitoring to balance microbial introduction from the must with controlled yeast activity.^[78]^[79]

In cider and perry

In cider production, apple must—obtained by crushing and pressing apples—is the primary substrate for fermentation, typically using either wild yeasts naturally present on the fruit skins and equipment or commercial yeast strains such as Saccharomyces uvarum or S. bayanus for more controlled outcomes.^[80] Wild yeast fermentations often yield complex, variable flavors but carry risks of off-flavors or stuck ferments, while commercial yeasts ensure consistency and complete conversion of sugars to alcohol.^[81] Cider styles vary based on fermentation extent: dry ciders result from full fermentation, reaching 10-12% alcohol by volume (ABV) as sugars are exhausted, whereas sweet styles involve arrested fermentation to retain residual sugars, typically around 5.5% ABV.^[80] Perry, the fermented beverage from pear must, shares similarities with cider but features distinct characteristics due to pears' higher tannin content, which contributes to greater astringency and structure in the final product. Perry pears, such as Barland or Butt varieties, often exhibit elevated tannin levels compared to cider apples, influencing mouthfeel and requiring careful blending with lower-tannin dessert pears like Comice for balance.^[82] Fermentation in perry typically proceeds more slowly, lasting 3-6 months to allow natural settling and clarity, as the higher tannins and unfermentable sugars like sorbitol promote a protracted process that enhances stability without filtration.^[82] Must for both cider and perry is frequently amended with additives to optimize fermentation and flavor; sugars such as sucrose or glucose syrup are added to boost potential alcohol content and sweetness, while acids like malic are incorporated to adjust pH and acidity for microbial stability.^[81]^[80] Carbonation is commonly achieved through secondary fermentation in bottles or tanks, where added sugars feed residual yeast activity to produce natural CO₂, distinguishing traditional sparkling styles from force-carbonated industrial products.^[80] Regional variations highlight must handling differences: in traditional English keeved cider, the must undergoes keeving—a process where pectin binds nutrients to limit yeast activity—retaining natural cloudiness and enabling a slow, incomplete fermentation for semi-sweet, sparkling results without added sugars.^[83] In contrast, French methods often clarify the must early through settling or fining to promote rapid, clean fermentation and early bottling.^[84] Production scale influences must extraction: artisanal methods rely on manual rack-and-cloth or basket presses, yielding about 70% juice from apples while preserving flavor complexity through gentle pressure, whereas industrial operations employ hydraulic or pneumatic presses—and occasionally centrifuges for pomace dewatering—to achieve higher yields up to 80% and process large volumes efficiently.^[85]^[86]

Culinary uses

Historical applications

In ancient Greece and Rome, boiled grape must was a primary sweetener and preservative in culinary applications, reduced to syrups known as defrutum (a one-third reduction) and sapa (a two-thirds reduction) for use in sauces, desserts, and meat dishes. These syrups provided a concentrated source of sweetness derived from natural grape sugars, often flavored with spices or herbs to enhance flavors in savory preparations. In Roman cuisine, defrutum and sapa appeared frequently in recipes alongside fermented fish sauce (garum), such as in stews and sauces where the syrup balanced umami and acidity. The production of these syrups involved boiling fresh grape must in large vessels to evaporate water and concentrate sugars, reducing the volume to one-third for sapa to ensure long-term preservation without fermentation. Solar evaporation was occasionally employed in sunnier regions to gently reduce must before final boiling, minimizing spoilage during processing. The resulting syrups were stored in sealed amphorae, often coated with resin to prevent leakage and contamination, allowing transport and year-round use in households and trade. A key cultural role for these must-derived syrups is evident in the Roman cookbook Apicius (compiled in the late 4th or early 5th century CE), where defrutum features in dishes like braised pork, glazed biscuits, and steak sauces, underscoring its versatility in elite banquets.^[87] In the Byzantine Empire, concentrated grape must known as pekmez served as a traded commodity, taxed as an unfermented syrup and integral to regional economies for sweetening and preservation.^[88] During the medieval period in Europe, must-based sweeteners persisted in spiced wines like hypocras, where reduced grape syrups complemented spices such as cinnamon and ginger to create medicinal and festive beverages.^[89] In the Middle East, dibs—a thick syrup from boiled grape or date must—functioned as a staple sweetener predating widespread honey or cane sugar use, applied in desserts, breads, and preserves across Levantine cuisines.^[90] The prominence of must syrups in cooking declined after the 15th century, as European imports of refined cane sugar from the Americas and Asia provided a cheaper, more stable alternative, gradually supplanting local fruit-based concentrates in both elite and everyday applications.^[91]

Modern applications

In contemporary culinary practices, grape must serves as a foundational ingredient in the production of traditional balsamic vinegar, particularly Aceto Balsamico di Modena PDO, which is crafted exclusively from cooked and concentrated must derived from Trebbiano, Lambrusco, and Ancellotta grapes grown in the Modena region.^[92] This must undergoes slow aging in a series of wooden barrels for a minimum of 12 years, often extending to over 100 years, resulting in a dense, syrupy condiment prized for its complex sweet-sour profile and used to enhance salads, cheeses, and desserts.^[93] The process preserves the must's natural sugars and acidity, making it a staple in Italian gastronomy since its protected designation in 2000.^[94] Grape must is also transformed into versatile syrups across various cultures, providing a natural sweetener for both sweet and savory dishes. In Greece, petimezi is produced by boiling down fresh grape must and is commonly drizzled over yogurt, pastries, or used in marinades.^[95] This thick molasses-like syrup maintains the fruit's caramelized notes and has seen renewed popularity in modern Mediterranean diets for its antioxidant properties.^[96] Similarly, in Turkey, pekmez—made by concentrating grape must through evaporation—features prominently in baklava, where it replaces or complements sugar syrup to bind layers of phyllo and nuts, offering a deeper, fruit-forward sweetness in this iconic dessert.^[97] In South Africa, moskonfyt, a reduced grape must syrup traditionally from Hanepoot grapes, is a key filling for tarts and bobotie, evoking colonial-era recipes while adapting to contemporary baking with its tangy viscosity.^[98] On the savory side, reduced grape must forms the base for glazes that elevate roasted or grilled meats, such as pork or lamb, by caramelizing during cooking to create a glossy, umami-rich coating often balanced with vinegar or spices.^[99] In Italian cuisine, mostarda di frutta incorporates grape must as a cooking syrup for candied fruits like quince or pears, infused with mustard essence to yield a piquant relish served alongside boiled meats or cheeses, with regional variations from Cremona emphasizing its sweet-heat contrast.^[100] Innovations in culinary techniques have expanded must's role, particularly through reductions applied in molecular gastronomy to intensify flavors in foams, gels, or emulsions, where concentrated must enhances wine-based sauces without overpowering delicate proteins.^[101] Additionally, unpasteurized grape must products have gained traction under organic certifications, such as USDA Organic standards, which require 100% certified organic grapes and prohibit synthetic additives, appealing to health-conscious consumers seeking raw, enzyme-rich ingredients for home cooking or fermentation.^[102] Global trade in grape must derivatives underscores its modern economic significance, with Italy exporting approximately 19 million liters in 2024, primarily as concentrated must for vinegar and syrup production, while Greece contributes through petimezi and related exports to European and North American markets.^[103] To facilitate this commerce, many products are pasteurized for shelf stability, extending usability up to several years without refrigeration, as seen in pure must concentrates that retain varietal aromas for winemaking or direct culinary use.^[104]

Health and historical aspects

Roman lead poisoning hypothesis

The Roman lead poisoning hypothesis posits that chronic exposure to lead, particularly through the processing of must into sweetened grape syrups, contributed significantly to health issues among the Roman elite and possibly to the empire's decline. Proposed by geochemist Jerome O. Nriagu in 1983, the theory highlights the practice of boiling must in lead vessels or lead-lined pots to produce defrutum (reduced to half volume) or sapa (reduced to one-third volume), which concentrated lead acetate—a sweet compound known as "sugar of lead"—used as a sweetener and preservative in wine and cuisine.^[105] Roman agricultural writers like Columella, in his 1st-century CE treatise De Re Rustica, explicitly recommended lead pots for this reduction process, noting their superiority for achieving a desirable sweetness, while Pliny the Elder and Cato also referenced similar methods.^[106] Supporting evidence comes from archaeometric analyses of Roman skeletal remains, which reveal elevated lead levels in bones, often up to 70-100 times higher than modern pre-industrial baselines, indicating widespread chronic exposure among urban and elite populations. For instance, studies of femora from Roman-era burials in Londinium show median bone lead concentrations of 26.5 μg/g, exceeding toxic thresholds and correlating with reduced fertility and infant mortality. Nriagu estimated that defrutum and sapa could account for 5-10% of daily lead intake for heavy wine consumers—such as aristocrats imbibing up to 2 liters daily—leading to blood lead levels around 50 μg/dL, sufficient to cause symptoms like gout (termed "saturnine gout"), infertility, anemia, and cognitive impairment. These effects disproportionately affected the upper classes, who favored sweetened wines, potentially exacerbating political instability through impaired decision-making among leaders.^[107]^[105] However, the hypothesis has faced substantial counterarguments from historians and scientists. Critics like John Scarborough and Hugo A. Waldron contended that Nriagu overstated the prevalence of lead vessels and misinterpreted literary sources, arguing that lead poisoning was not endemic but limited to specific occupations or accidental exposures. Recent isotopic analyses, such as a 2014 study of sediments from Rome's water systems, indicate that while lead pipes (fistulae) contributed to environmental contamination, their impact on potable water was minimal due to protective lime scales, suggesting plumbing as a dominant source over cookware if exposure occurred at all. Additionally, archaeological evidence points to copper or bronze vessels being more common for boiling must in many regions, with lead linings not universally applied, further diluting the role of sapa production in widespread toxicity.^[108]^[109] The legacy of Nriagu's hypothesis remains debated, with some scholars linking it to broader health declines in the late Roman Empire—such as increased gout prevalence among emperors—but others dismissing it as a minor factor amid economic, military, and epidemiological pressures. A January 2025 study in PNAS analyzed ice cores and found that atmospheric lead pollution peaked during the Roman Imperial period, resulting in average blood lead levels of 15–25 μg/dL empire-wide, potentially causing a 2.5–3 point IQ reduction and contributing to health issues like reduced fertility, though not solely responsible for the empire's fall. While it underscored the dangers of ancient metallurgical practices, modern reassessments emphasize multifactorial causes for Rome's fall rather than lead alone.^[105]^[110]^[111]

Nutritional profile

Grape must, the unfermented juice extracted from pressed grapes, is rich in antioxidants, particularly polyphenols derived from grape skins and seeds, which contribute to its potential health benefits. Resveratrol, a key stilbenoid polyphenol concentrated in grape skins at levels of 50–100 μg/g, is present in must at concentrations typically ranging from 1 to 5 mg/L, especially in red varieties where maceration allows greater extraction. These compounds, including flavonoids and anthocyanins, exhibit strong antioxidant properties that help combat oxidative stress. Additionally, must provides notable amounts of vitamins, such as vitamin C (12–30 mg/100 g in fresh grape juice equivalents) and vitamin K (approximately 0.2–0.3 μg/100 g), supporting immune function and blood clotting, respectively.^[112]^[113]^[114] The polyphenols in grape must have been linked to cardiovascular health improvements, with studies on purple grape juice—a close analog to fresh must—demonstrating enhanced endothelial function and reduced susceptibility of low-density lipoprotein (LDL) to oxidation in patients with coronary artery disease after short-term consumption. Research indicates that regular intake of polyphenol-rich grape products may lower cardiovascular disease risk by up to 20% through mechanisms like reduced inflammation and improved vascular reactivity, though direct must-specific trials are limited. Calorically, must derives 60–100 kcal per 100 mL primarily from natural sugars (15–20 g/100 mL), with negligible fat (0.1–0.3 g/100 mL) and protein (0.5–1 g/100 mL), making it a low-fat, carbohydrate-dense beverage.^[115]^[116] Despite these benefits, consuming grape must carries risks, particularly its high natural sugar content, which can elevate blood glucose levels and pose challenges for individuals with diabetes. Potential mycotoxin contamination, such as ochratoxin A from Aspergillus molds on damaged grapes, introduces hepatotoxic and nephrotoxic hazards, with levels varying by vineyard conditions and processing. Unpasteurized must also presents microbial risks, including pathogens like E. coli, due to possible fecal contamination during harvesting or pressing, necessitating pasteurization for safety.^[117]^[118] Recent research in the 2020s highlights must's prebiotic potential, with grape-derived fibers selectively enhancing beneficial gut microbiota such as Bifidobacterium in dysbiotic patients, promoting short-chain fatty acid production and intestinal barrier integrity. These findings support must's incorporation into functional foods, like fortified beverages, to leverage its fibers and polyphenols for gut health modulation. Compared to processed wine, fresher must retains higher levels of heat-sensitive nutrients like vitamin C and certain polyphenols, as fermentation and aging can degrade up to 50% of these compounds.^[119]^[120]^[121]

Religious and liturgical uses

In Christian liturgy

In Christian liturgy, particularly within the Roman Catholic Church, unfermented must—known as mustum—serves as a valid substitute for fermented wine in the Eucharist for individuals unable to consume alcohol due to medical conditions such as alcoholism or intolerance.^[122] This practice is governed by the 1983 Code of Canon Law, Canon 924 §3, which requires that the wine be natural, derived from grapes, pure, and incorrupt, a criterion that extends to mustum as grape juice either fresh or preserved by methods like freezing that suspend fermentation without altering its nature.^[123] The Congregation for Divine Worship and the Discipline of the Sacraments clarified in a 2017 circular letter that mustum constitutes valid matter for consecration, ensuring the sacrament's integrity while accommodating pastoral needs.^[122] Requirements for mustum emphasize its purity: it must be a natural, unpasteurized product from grapes with no additives beyond minimal preservatives like sulfites, and fermentation must be halted early, resulting in less than 1% alcohol content.^[124] In the United States, the United States Conference of Catholic Bishops (USCCB) has approved specific brands of mustum, such as those produced by Benedictine communities, for liturgical use after verifying compliance with these standards.^[124] Permission to use mustum is granted by the local ordinary (bishop) for priests celebrating Mass or for lay faithful receiving Communion, and this authority may be delegated to pastors under Canon 137 §1; no medical certification is required, but the permission applies only to those with a persistent condition.^[125] For minors or others without diagnosed intolerance, widespread use is prohibited, though individual dispensations may be considered for grave reasons.^[124] In practice, mustum is employed during the consecration of the Eucharist, where it undergoes transubstantiation—the Catholic doctrine by which its substance becomes the Blood of Christ while retaining the accidents of grape juice.^[122] It is typically stored as frozen concentrate to preserve its unfermented state without spoilage, then thawed and mixed with a small amount of water as required by liturgical norms.^[126] Debates on the validity of mustum versus fully fermented wine persist among some theologians and laity, centering on interpretations of scriptural references to "wine" (oinos in Greek, implying fermentation) and the potential for mustum to fully effect transubstantiation.^[127] However, official Church documents affirm its licitness, provided it meets the specified criteria, and its use remains rare but has increased alongside sobriety movements and greater awareness of alcohol-related health issues.^[124]

In other traditions

In Jewish tradition, must, or unfermented grape juice, serves as an acceptable alternative to wine in various liturgical rituals, particularly when alcoholic beverages are unsuitable for participants such as children or those observing health restrictions.^[128] This practice aligns with halakhic (Jewish legal) guidelines that prioritize the symbolic act of sanctification over fermentation, allowing grape juice to fulfill the mitzvah (commandment) of reciting blessings over grape products. A primary use occurs during Kiddush, the ritual blessing recited to usher in the Sabbath or Jewish holidays, where a cup of grape juice may be used to recite the blessing "Borei p'ri hagafen" (Who creates the fruit of the vine). Grape juice is especially common in home settings or educational contexts to include all family members, maintaining the ritual's communal and joyful essence without the effects of alcohol.^[129] During the Passover Seder, the consumption of four cups of wine or grape juice symbolizes the four expressions of redemption in the Exodus narrative from the Torah. Unfermented grape juice is permitted and often preferred in observant households to avoid any trace of chametz (leavened substances) or intoxication, ensuring the ritual's focus on storytelling and freedom remains undiluted; rabbinic authorities like Rabbi Moshe Feinstein have even ruled on blending minimal fermented elements with juice to enhance flavor while adhering to Passover laws.^[130] In the Havdalah ceremony concluding the Sabbath, grape juice can substitute for wine in the cup over which blessings are recited to separate the holy from the mundane, emphasizing sensory rituals like smelling spices and lighting a multi-wick candle. This flexibility underscores Judaism's emphasis on accessibility in worship, with kosher certification required for grape juice to prevent idolatrous associations from non-Jewish production processes, as mandated in texts like the Talmud (Avodah Zarah 29b).^[131] Beyond these core rituals, grape juice appears in lifecycle events such as brit milah (circumcision ceremonies) or aufruf (pre-wedding Sabbath honors), where it supports the festive shehecheyanu blessing, though wine remains the traditional preference for its deeper symbolic ties to joy and covenant in Jewish theology. In many Protestant Christian denominations, particularly those influenced by the 19th-century temperance movement, unfermented grape juice is used instead of wine for the Lord's Supper or Holy Communion. This practice, popularized by Thomas Bramwell Welch in 1869 who developed a pasteurization method to prevent fermentation, allows participants to commemorate the Last Supper without alcohol, aligning with abstinence principles while fulfilling the biblical command to use "fruit of the vine."^[132]

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Made uniquely from our pure fresh grape must that has been pasteurised to provide you with a premium shelf-stable product – NO water added or to be added.
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Antioxidant capacity, total phenolic, carotenoid, and vitamin C ...
Grapes are known to be a nutrient source of vitamin C in dietary intake. Here, the ascorbic acid content ranged from 12.33 ± 0.01 (Victoria) to 30.80 ± 4.98 mg/ ...Missing: vitamins must
[119]
Purple Grape Juice Improves Endothelial Function and Reduces the ...
Conclusions—Short-term ingestion of purple grape juice improves FMD and reduces LDL susceptibility to oxidation in CAD patients. Improved endothelium-dependent ...
[120]
Grape Polyphenols' Effects in Human Cardiovascular Diseases and ...
Grape polyphenols may improve vascular health, reduce CVD risk, and have antioxidant, anti-inflammatory, and anti-ageing properties. They may also help with ...Grape Polyphenols' Effects... · 2. Cardiovascular Disease · 2.3. Ldl Oxidation And...
[121]
Review of Mycotoxins in Grapes and Grape Products - IntechOpen
6. Conclusions. The presence of fungal contamination in vineyards can elevate health risks attributed to mycotoxins in grapes and their respective products. ...
[122]
Fungal and mycotoxin problems in grape juice and wine industries
The toxic effects of these mycotoxins are related to nephrotoxicity, hepatotoxicity, cytotoxicity, teratogenicity, and immunotoxicity [14]. Some mycotoxins are ...
[123]
A Novel Grape-Derived Prebiotic Selectively Enhances Abundance ...
Mar 23, 2021 · This study aimed to evaluate the capacity of Previpect, a new prebiotic obtained from grapes fibre, to balance the dysbiosis found in patients ...
[124]
Prebiotic Effects of California Grapes on Gut Health and ...
Apr 20, 2025 · The goal of this clinical trial is to assess the impact of table grape consumption on gut microbiome, intestinal permeability, ...
[125]
Grape Juice: Nutrients, Benefits, Downsides & More - Healthline
Mar 15, 2022 · One cup (237 mL) of unsweetened purple grape juice contains the following nutrients ( 3 ): Calories: 152.
[126]
Circular letter to Bishops on the bread and wine for the Eucharist
Jun 15, 2017 · b) “Mustum, which is grape juice that is either fresh or preserved by methods that suspend its fermentation without altering its nature (for ...
[127]
Code of Canon Law - Function of the Church Liber (Cann. 879-958)
924 §1. The most holy eucharistic sacrifice must be offered with bread and with wine in which a little water must be mixed. §2. The bread must be only wheat ...
[128]
Celiac Disease, Alcohol Intolerance, and the Church's Pastoral ...
The authority to permit the lay faithful to use mustum and low-gluten hosts in the reception of Holy Communion may be delegated to pastors under canon 137 §1 of ...
[129]
Circular Letter to all Presidents of the Episcopal Conferences ...
Jul 24, 2003 · The Ordinary is competent to give permission for an individual priest or layperson to use low-gluten hosts or mustum for the celebration of the ...
[130]
None
### Summary on Mustum in Catholic Liturgy
[131]
The Tradition of Using Wine in the Eucharist - Facebook
Apr 2, 2025 · One solution was to squeeze grapes during the week and serve the juice before it fermented. Grapes were not readily available to every church and a of churches ...Which Greek word is most appropriate to use for unfermented grape ...Is the 'new wine' in the Bible fermented? Also, is there an early ...More results from www.facebook.com
[132]
What Counts as Valid Wine for the Eucharist? - Catholic Answers
Jun 14, 2023 · Mustum, which is grape juice that is either fresh or preserved by methods that suspend its fermentation without altering its nature (for example ...Missing: guidelines | Show results with:guidelines
[133]
13 Facts About Judaism and Wine - Chabad.org
Grape Juice Is a Good Stand-In. While bona fide alcoholic wine is preferred for Kiddush and most other ceremonial purposes, especially the Passover Seder, grape ...
[134]
https://www.reclaimingjudaism.org/teachings/must-we-use-wine-jewish-ritual
[135]
Four Cups of Wine: A Path to Freedom - The Schechter Institutes
Mar 13, 2010 · Rabbi Moshe Feinstein, for example, ruled that on Passover one must mix in a small amount of fermented wine with the grape juice in order to ...
[136]
The Unique Kosher Status of Grape Juice and Wine
Jul 3, 2025 · In Jewish tradition, wine and grape juice are more than just ingredients. They hold deep religious and symbolic meaning, which is why they are ...Missing: (unfermented Islam, Hinduism, pagan