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Microbial fuel cell

A microbial fuel cell (MFC) is a bio-electrochemical system that harnesses microorganisms to convert chemical energy from organic substrates, such as wastewater, into electrical energy through anodic oxidation and cathodic reduction reactions under anaerobic conditions.^[1] These devices, first demonstrated in 1911 by M.C. Potter, who showed microbial generation of electricity from organic matter, have evolved into promising technologies for sustainable energy production and environmental remediation.^[2] In an MFC, electroactive bacteria, such as Geobacter sulfurreducens or Shewanella putrefaciens, form biofilms on the anode, where they oxidize organic compounds like glucose or acetate, releasing electrons that travel through an external circuit to the cathode, while protons migrate through a separator, such as a proton exchange membrane (PEM), often Nafion, to complete the circuit and generate current.^[3] Key components include the anode and cathode electrodes, typically made from carbon-based materials like cloth or felt for high surface area and conductivity, the microbial inoculum, and an electron acceptor at the cathode, usually oxygen reduced to water in aerobic setups.^[1] MFCs have applications in wastewater treatment, achieving up to 80% chemical oxygen demand (COD) removal while producing bioelectricity, as well as in biosensors for detecting biochemical oxygen demand (BOD) and resource recovery from wastes.^[3] Current densities have improved from a few μA/cm² in early 2000s prototypes to several mA/cm² as of 2023, with power densities reaching up to 13 mW/cm² in recent 2024 studies; innovations like nanotechnology-enhanced electrodes, ceramic membranes, and genetic engineering of microbes have significantly boosted efficiency.^[1]^[4] Despite progress, challenges include low overall energy output, electrode scalability, and bacterial kinetics limitations, with ongoing research as of 2025 pointing to integration in renewable energy systems and commercialization, with the market valued at $8 million.^[2]^[5]

Introduction

Definition

A microbial fuel cell (MFC) is a bio-electrochemical system that drives an electric current by using bacteria and a high surface-area electrode to oxidize organic substrates, with electrons transferred to the electrode rather than oxygen.^[6] This process harnesses microbial metabolism to convert chemical energy from organic matter directly into electrical energy, offering a sustainable alternative for energy recovery from waste.^[3] At the anode, microorganisms oxidize organic matter—such as acetate—to carbon dioxide, releasing electrons that flow through an external circuit and protons that migrate to the cathode.^[7] In the cathode, these electrons participate in the reduction of an electron acceptor, typically oxygen to water, completing the circuit and generating power.^[8] Unlike traditional fuel cells, which rely on abiotic chemical catalysts like platinum, MFCs employ live microorganisms as biocatalysts, enabling operation on complex, untreated waste substrates such as wastewater.^[9] The fundamental structure consists of an anode chamber colonized by a microbial biofilm, a cathode chamber, and a proton-exchange separator that facilitates ion transfer while preventing short-circuiting.^[10]

Basic Components

A microbial fuel cell (MFC) comprises several core physical and material elements designed to support the electrochemical processes inherent to its function. These include the anode and cathode electrodes, a proton exchange membrane (PEM) or separator, the microbial inoculum, and the overall reactor configuration, each selected for their ability to maintain separation, conductivity, and biological compatibility in the system.^[11]^[3] The anode, positioned in the anaerobic compartment, acts as the primary site for microbial attachment and electron collection, requiring materials with high surface area to foster biofilm development. Carbon-based materials dominate anode construction due to their conductivity, chemical stability, and cost-effectiveness; representative examples include carbon cloth, graphite rods or plates, carbon felt, and carbon brushes, which provide extensive surface area—often exceeding 1000 m²/m³—for bacterial colonization.^[11]^[3]^[7] Stainless steel mesh and advanced composites like graphene-modified carbons have also been employed to enhance durability and electron transfer efficiency in practical setups.^[11] These choices prioritize biocompatibility to support long-term microbial adhesion without toxicity.^[3] In contrast, the cathode facilitates the reception of electrons from the external circuit, typically interfacing with an aerobic environment or electron acceptor like oxygen. It is commonly constructed from carbon cloth or paper for its porosity and electrical properties, often enhanced with catalysts such as platinum (at loadings of 0.1–0.5 mg/cm²) to promote oxygen reduction, though non-precious alternatives like manganese dioxide (MnO₂) or activated carbon are increasingly used to reduce costs.^[11]^[3]^[7] Air-cathode designs, featuring a waterproof layer like polytetrafluoroethylene (PTFE), enable passive oxygen diffusion from ambient air, simplifying operation in single-chamber systems.^[11] Stainless steel mesh cathodes provide mechanical robustness for scaled prototypes.^[3] The proton exchange membrane (PEM) or alternative separator is crucial for compartmentalization, permitting selective ion transport while blocking unwanted crossover of substrates or oxygen. Nafion, a perfluorosulfonic acid polymer, is the most widely adopted PEM material owing to its high proton conductivity (up to 0.1 S/cm) and chemical resistance, though its high cost (around $500/m²) has prompted alternatives like sulfonated polyether ether ketone (SPEEK), ceramic membranes, or ultrafiltration fabrics such as chitosan or cellulose.^[11]^[3]^[7] In mediatorless designs, porous separators like glass fiber filters maintain physical division without active ion exchange.^[11] These materials ensure efficient proton migration (H⁺ ions) across the cell while minimizing short-circuiting.^[3] The microbial inoculum introduces the electroactive microorganisms essential for anode colonization, typically sourced from natural or waste environments rich in exoelectrogens. Common sources include anaerobic wastewater sludge, activated sludge from treatment plants, marine sediments, or soil, which harbor diverse bacterial consortia capable of extracellular electron transfer.^[11]^[3]^[7] Key examples of exoelectrogens include Geobacter sulfurreducens, known for its metal-reducing capabilities and prevalence in sediments, and Shewanella oneidensis, which thrives in mixed cultures from wastewater; pure cultures of these species have been used in lab-scale MFCs, but mixed inocula from sludge often yield more robust biofilms due to synergistic interactions.^[11]^[3] Inoculation typically involves adding 10–20% (v/v) of the source material to the anode chamber, allowing enrichment over weeks.^[7] Reactor configurations dictate the spatial arrangement of these components, influencing scalability and ease of assembly. Dual-chamber setups, separated by a PEM, provide isolated anaerobic (anode) and aerobic (cathode) environments for precise control, often using H-shaped or flat-plate designs with volumes of 100–500 mL in bench-scale tests.^[11]^[3] Single-chamber configurations eliminate the PEM for cost savings (reducing material expenses by up to 50%), relying on an air-cathode exposed to atmosphere, as seen in tubular or flat-pack prototypes suitable for continuous flow.^[11]^[7] Stackable or modular designs connect multiple units in series or parallel via conductive wiring, enabling voltage multiplication (e.g., 0.6 V per cell to 10 V in stacks of 16), and are exemplified by ceramic-supported MFCs for wastewater integration.^[3]^[7] These variations allow adaptation to specific scales, from lab prototypes to pilot systems.^[11]

History

Early Discoveries

The concept of bioelectricity, which indirectly inspired later work on microbial energy generation, traces back to the 19th century, particularly Michael Faraday's investigations into the electrical discharges of electric eels (Electrophorus electricus) in 1838–1839. Faraday's experiments demonstrated how biological tissues could produce significant electrical potentials, akin to galvanic batteries, through the arrangement of specialized organs that generated voltages up to 600 volts, though with low current. These observations highlighted the potential for living systems to harness electrochemical processes, laying foundational ideas for bioelectrochemical systems despite focusing on animal physiology rather than microorganisms. The first direct demonstration of electricity production from microbial metabolism occurred in 1911, when British botanist M.C. Potter reported electrical effects during the decomposition of organic compounds by microorganisms. Potter constructed a simple galvanic cell using platinum electrodes immersed in cultures of Saccharomyces cerevisiae (baker's yeast) and unspecified bacteria in a nutrient medium containing glucose or other organics. He observed electromotive forces (E.M.F.) ranging from 0.2 to 1.0 volts per cell, with polarity indicating the electrode in the microbial culture as the anode, confirming that microbial oxidation processes liberated electrical energy alongside heat. This pioneering setup marked the initial recognition of microbes as potential biocatalysts for electricity generation, though the yields were modest and the mechanism remained unclear.^[12] Building on Potter's findings, research in the 1930s and 1940s advanced the understanding of bacterial contributions to fuel cell-like systems. In 1931, American microbiologist Barnett Cohen developed microbial half-cells using Escherichia coli suspensions, demonstrating that bacterial respiration could drive anodic reactions. By connecting 24 such half-cells in series, Cohen achieved a total voltage of over 35 volts at a current of about 2 milliamperes, verifying that microbial oxidation of organics served as the electron source without requiring external mediators. These experiments, conducted during a period of growing interest in bioenergetics, confirmed the reproducibility of Potter's observations but highlighted persistent challenges, including low power densities (typically below 1 mW/m²) that limited practical utility. Early efforts to apply these systems, such as exploratory patents for "microbial batteries" in the 1930s and potential wartime considerations for remote, low-maintenance power during World War II, were hampered by inefficient electron transfer and material limitations, relegating the technology to academic curiosity until later decades.

Key Developments

In the 1960s, NASA initiated research into microbial fuel cells (MFCs) for space missions, focusing on their potential to convert astronaut waste into electricity within closed-loop life support systems.^[13] This interest stemmed from the need for sustainable power generation in extraterrestrial environments, where traditional fuel sources were impractical.^[14] By the 1970s, practical applications expanded; in 1977, Karube et al. developed the first MFC-based biosensor using immobilized Clostridium butyricum to measure biochemical oxygen demand (BOD) in wastewater, enabling real-time monitoring of organic pollution levels.^[15] The 1980s and 1990s marked foundational microbiological advances, with the discovery of electroactive bacteria capable of extracellular electron transfer. Shewanella oneidensis MR-1 was isolated in 1988 from Lake Oneida, New York, revealing its ability to reduce metals and transfer electrons directly to electrodes.^[16] Similarly, Geobacter metallireducens was identified around the same period, demonstrating efficient metal reduction and paving the way for mediatorless designs.^[17] Concurrently, Bennetto's work advanced mediated MFC systems by incorporating redox compounds like neutral red as electron shuttles, improving current output from bacterial metabolism of substrates such as glucose. The 2000s saw a surge in MFC research, fueled by global emphasis on renewable energy. A pivotal 1993 study by Allen and Bennetto demonstrated mediatorless electricity generation from carbohydrates and proteins using mixed bacterial cultures on graphite electrodes, achieving power densities up to 0.1 mW/cm² without chemical mediators. This design reduced costs and toxicity concerns, spurring innovation. Post-2000, annual publications on MFCs grew exponentially—from fewer than 50 in 2000 to over 1,000 by 2010—driven by applications in sustainable energy and bioremediation.^[18] From the 2010s onward, MFCs integrated with microbial electrolysis cells (MECs) to enable hydrogen production from organic waste, where MFC-generated electricity powered MEC cathodes for water splitting, yielding up to 0.8 m³ H₂/m³ reactor volume per day in stacked systems.^[19] Scaling efforts advanced through companies like Cambrian Innovation, which deployed modular MFC systems for industrial wastewater treatment, recovering energy while reducing chemical oxygen demand by over 80% in brewery applications.^[20] Acquired in 2023, the firm exemplified the shift toward commercial viability.^[21] In the 2020s up to 2025, focus intensified on 3D-printed electrodes, such as those incorporating carbon nano fibers in microfluidic benthic MFCs, achieving power densities up to 10 mW/m² through enhanced conductivity, surface area, and biofilm adhesion.^[22] Genetic engineering of microbes, including Shewanella strains to enhance extracellular electron transfer pathways, improved MFC efficiencies.^[23] Recent advances as of 2025 include AI-optimized microbial consortia for better performance in pilot-scale wastewater treatment systems.^[24]

Operating Principles

Electron Transfer Mechanisms

In microbial fuel cells (MFCs), electron transfer from microorganisms to the anode occurs primarily through extracellular electron transfer (EET) processes, enabling the conversion of chemical energy from organic substrates into electrical current. EET pathways allow electroactive bacteria to donate electrons generated during anaerobic metabolism directly or indirectly to the electrode surface, bypassing the limitations of traditional intracellular electron transport chains. These mechanisms are essential for sustaining current production and have been extensively studied in model organisms like Geobacter and Shewanella species.^[25] EET is broadly categorized into direct and indirect modes. Direct EET involves physical contact between the bacterial cell or biofilm and the anode, facilitated by specialized protein complexes such as outer membrane cytochromes and conductive pili (nanowires). In Geobacter sulfurreducens, for instance, the outer membrane cytochrome OmcS serves as a key terminal reductase, enabling long-range electron transfer through the biofilm to the electrode; related studies demonstrate localization of cytochromes like OmcZ at the biofilm-electrode interface for efficient conduction. Recent research (as of 2022) has further shown that OmcZ forms conductive nanowire structures that support electron transfer in thick biofilms.^[26]^[27] Conversely, indirect EET relies on diffusible redox mediators secreted by the bacteria to shuttle electrons across the cell envelope. Shewanella oneidensis exemplifies this through the production of flavins, including riboflavin and flavin mononucleotide, which bind to outer membrane proteins like MtrC and facilitate electron transfer to extracellular acceptors like electrodes.^[17] Mediated electron transfer employs exogenous chemical mediators to bridge intracellular electron generation and the anode, particularly useful for non-electroactive microbes lacking natural EET capabilities. These mediators, such as methylene blue, are reduced by accepting electrons from the bacterial respiratory chain during substrate oxidation and then oxidized at the electrode, thereby enhancing electron flux. Early demonstrations with methylene blue in yeast-based MFCs showed power densities up to several hundred mW/m² by improving shuttle kinetics, though mediator toxicity and cost limit widespread adoption.^[28] At the core of these EET processes lies anaerobic respiration, where electroactive bacteria oxidize organic substrates like glucose in the anodic compartment under oxygen-limited conditions. Glucose is metabolized via glycolysis to pyruvate, followed by decarboxylation and further oxidation through the tricarboxylic acid cycle or fermentative pathways, ultimately producing CO₂, protons, and electrons for transfer to the anode. The anodic half-reaction can be represented as:

\text{C}_6\text{H}_{12}\text{O}_6 + 6\text{H}_2\text{O} \rightarrow 6\text{CO}_2 + 24\text{H}^+ + 24\text{e}^-

The overall theoretical MFC reaction, combining anode and aerobic cathode, is:

\text{C}_6\text{H}_{12}\text{O}_6 + 6\text{O}_2 \rightarrow 6\text{CO}_2 + 6\text{H}_2\text{O}

However, microbial efficiencies typically capture only 20-50% of theoretical electrons due to competing metabolic losses like biomass synthesis and side reactions. The driving force for these transfers is influenced by the Nernst equation for electrode potential:

E = E^0 - \frac{RT}{nF} \ln Q

where E is the cell potential, E^0 the standard potential, R the gas constant, T the temperature in Kelvin, n the number of electrons transferred, F Faraday's constant, and Q the reaction quotient; this highlights how shifts in substrate or product concentrations modulate EET thermodynamics.^[29] Several environmental and biological factors modulate EET efficiency. pH affects proton gradients and cytochrome functionality, with neutral pH (around 7) optimizing Geobacter performance by maintaining respiratory chain activity. Temperature influences enzyme kinetics and membrane permeability, with mesophilic ranges of 30-35°C maximizing electron yields in Shewanella systems, while extremes reduce metabolic rates. Biofilm thickness plays a critical role in direct EET; Geobacter forms highly conductive biofilms up to ~130 μm thick via nanowires, enabling efficient long-range transfer, though very thick layers may introduce diffusion limitations.^[30]^[27]

Electricity Generation Process

In a microbial fuel cell (MFC), electricity generation begins at the anode, where electroactive microorganisms oxidize organic substrates, such as acetate, in an anaerobic environment. This anodic reaction releases electrons and protons; for example, the oxidation of acetate proceeds as CH₃COO⁻ + 2H₂O → 2CO₂ + 7H⁺ + 8e⁻.^[31] The electrons are transferred to the anode surface through mechanisms involving microbial outer membranes or mediators, while the protons (H⁺ ions) are released into the anolyte.^[32] The protons then migrate from the anodic chamber to the cathodic chamber through a proton-exchange membrane or separator, maintaining charge balance across the cell.^[31] At the cathode, these protons combine with the electrons and an electron acceptor to complete the reduction reaction. In aerobic MFCs, the typical cathodic reaction uses oxygen as the acceptor: O₂ + 4H⁺ + 4e⁻ → 2H₂O.^[32] Alternative acceptors, such as ferricyanide, can be employed in mediator-based systems: [Fe(CN)₆]³⁻ + e⁻ → [Fe(CN)₆]⁴⁻, which often enhances performance in laboratory setups.^[31] Electrons released at the anode flow externally through a circuit connected to a load, such as a resistor, generating electrical current and completing the circuit. This process produces an open-circuit voltage typically ranging from 0.3 to 0.8 V per cell, depending on the substrates, microbial community, and electrode materials.^[32] The resulting power output is quantified by metrics including current density (often 1–10 A/m² in optimized systems), power density (up to 1–4 W/m² for advanced designs), and Coulombic efficiency, which measures the fraction of substrate-derived electrons captured as current (ranging from 10% to 90%, influenced by competing microbial processes like methanogenesis).^[31]^[32] The startup phase of an MFC is critical for establishing efficient electricity generation, involving the inoculation of the anode with a microbial consortium (e.g., from wastewater sludge) and a period of enrichment lasting days to weeks. During this time, a biofilm forms on the anode, dominated by exoelectrogenic bacteria such as Geobacter species, which adapt to the electrode environment and begin sustained substrate oxidation.^[31] This acclimation typically occurs in fed-batch mode with simple substrates like acetate to promote selective growth of electroactive populations.^[32]

Types

Mediated and Mediatorless Designs

Microbial fuel cells (MFCs) are primarily classified into mediated and mediatorless designs, distinguished by the mechanism of electron transfer from microbial metabolism to the anode electrode. Mediated MFCs utilize exogenous chemical mediators to facilitate electron shuttling from intracellular bacterial processes to the anode surface. These synthetic compounds, such as phenazines and thionines, enter the bacterial cell to intercept electrons from the respiratory chain and subsequently oxidize at the electrode, enabling current generation. This configuration was first demonstrated in the 1980s by Bennetto et al., who reported electricity production from Proteus vulgaris using thionine as a redox mediator in a dual-chamber setup.^[33] Mediated designs offer the advantage of enhanced initial electron transfer efficiency, particularly with non-electroactive bacteria, yielding power densities typically in the range of 10–100 mW/m².^[7] However, drawbacks include mediator toxicity to microbial communities, high production costs, and chemical instability leading to degradation over time, which hampers long-term performance and scalability.^[2] Mediatorless MFCs, by contrast, depend on direct electron transfer (DET) via physical contact between bacterial cells and the anode or through endogenous mediators secreted by the microbes, avoiding synthetic additives. Electroactive bacteria such as Shewanella oneidensis achieve DET through outer-membrane proteins like cytochromes, while flavins (e.g., riboflavin and flavin mononucleotide) serve as natural shuttles to bridge the cell-electrode gap. The seminal demonstration of a mediatorless MFC occurred in 1999 with Shewanella putrefaciens, where direct electron flow to a graphite electrode produced measurable current without added mediators. These systems promote sustainability by eliminating chemical inputs, reducing toxicity risks and operational costs, though they often require longer startup periods for biofilm formation and may exhibit lower initial power outputs. Optimized mediatorless MFCs with mature biofilms can achieve power densities up to 1 W/m². For example, S. oneidensis secretes flavins that significantly boost extracellular electron transfer rates in such configurations.^[34] In comparison, mediated MFCs excel in rapid deployment and higher short-term power generation, but mediatorless designs have dominated research since the late 1990s due to their environmental compatibility and potential for practical, chemical-free operation. This historical transition underscores a focus on leveraging innate bacterial electrogenicity for more viable bioelectrochemical systems.^[2]

Specialized Configurations

Soil-based microbial fuel cells leverage natural soil as both electrolyte and substrate, harnessing organic matter decomposition for electricity generation without requiring synthetic media. In plant-microbial fuel cell (plant-MFC) hybrids, living plants enhance performance by releasing root exudates—such as sugars and organic acids—as substrates for electroactive bacteria in the rhizosphere, enabling continuous energy harvesting. These configurations typically eliminate the need for a proton exchange membrane, simplifying design and lowering costs, while achieving power densities of 10–100 μW/cm² depending on plant species and soil conditions. For instance, systems with wetland plants like Spartina anglica have sustained outputs for over a year, powering small sensors in agricultural or wetland settings.^[35]^[36]^[37] Phototrophic biofilm microbial fuel cells integrate photosynthetic microorganisms, such as algae or cyanobacteria, directly into the cathode biofilm to produce oxygen via photosynthesis, thereby replacing mechanical aeration and facilitating in situ CO₂ fixation. This setup yields dual outputs of bioelectricity and algal biomass, with the biofilm enhancing electron transfer and cathode kinetics. Research demonstrates that oxygenic phototrophic biofilms can double current densities compared to non-biological cathodes, reaching up to 0.14 A/m², while also supporting nutrient recovery from wastewater. Revolving algae biofilm designs further optimize mass transfer, achieving simultaneous electricity generation and microalgal biomass production exceeding 30 g/m².^[38]^[39]^[39] Microbial electrolysis cells (MECs) represent an extension of microbial fuel cell technology, where an external low-voltage input (typically 0.5–1 V) supplements the bioelectrochemical process to produce hydrogen gas from organic substrates at the cathode, rather than relying solely on spontaneous electricity generation. Sharing the same exoelectrogenic anode microbes as MFCs, MECs prioritize hydrogen evolution over power output, with anodic oxidation driving proton and electron production for cathodic reduction. These systems achieve hydrogen recovery efficiencies of 70–90% and are applied in wastewater treatment, converting chemical oxygen demand reductions of 80–95% into valuable biofuel.^[40]^[41]^[42] Membrane-based microbial fuel cells incorporate advanced separators, such as nanoporous ceramics or graphene composites, to minimize biofouling, enhance proton selectivity, and support scalable geometries like tubular designs. Ceramic membranes, often made from materials like alumina or zirconia, provide mechanical robustness and reduced internal resistance, enabling continuous operation in high-fouling environments. Tubular ceramic configurations have demonstrated chemical oxygen demand removals up to 92% and power densities of 0.1–0.3 W/m², facilitating easier integration into stacked systems for larger-scale deployment. Graphene-modified membranes further improve durability and ion flux, mitigating ohmic losses in long-term applications.^[43]^[44]^[45] Sediment microbial fuel cells deploy anodes in anaerobic marine or riverbed sediments and cathodes in overlying aerobic water, exploiting natural geochemical gradients for passive electricity production without external substrates. These benthic systems generate low but sustained power (10–100 mW/m²) to drive underwater sensors for environmental monitoring, such as temperature or pH probes, over periods exceeding 500 days. Additionally, they mitigate sediment pollution by oxidizing sulfides and heavy metals, improving benthic habitats through enhanced microbial activity at the anode.^[46]^[47]^[48] Stacked microbial fuel cells arrange multiple unit cells in series or parallel to amplify voltage and power, addressing limitations of single-cell outputs for practical applications. Ceramic-based stacks, for example, have produced up to 1.5 V and 50 mW total power in standard mode, with supercapacitive operation enabling pulsed energy storage. Microbial desalination cells (MDCs), a stacked variant, insert a saline chamber flanked by ion-exchange membranes between the anode and cathode, enabling simultaneous desalination and power generation. Stacked MDCs achieve salt removals of 90–99% from seawater while maintaining power densities of 0.5–2 W/m², comparable to conventional MFCs.^[44]^[49]^[50]

Applications

Wastewater Treatment and Bioremediation

Microbial fuel cells (MFCs) offer a promising approach for treating domestic and industrial wastewater by leveraging electrogenic bacteria to degrade organic matter while generating electricity. In these systems, wastewater serves as the substrate in the anodic chamber, where microbes oxidize organics such as carbohydrates and proteins, transferring electrons to the anode and thereby reducing chemical oxygen demand (COD). Studies have demonstrated COD removal efficiencies ranging from 65-70% in long-term operations to over 80% under optimized conditions with high organic loading, making MFCs suitable for effluents like municipal sewage and food processing waste.^[51]^[52] Pilot-scale implementations have validated these capabilities, particularly at institutions like Pennsylvania State University, where modular MFC systems installed at wastewater treatment plants in the 2010s achieved consistent pollutant removal and power output from real effluents. For instance, a 6.2 L air-cathode MFC processed domestic wastewater, achieving a power density of 6.3 W/m³.^[53]^[54] Beyond basic organic treatment, MFCs facilitate bioremediation of recalcitrant pollutants, including azo dyes and pharmaceuticals, through anodic or cathodic microbial processes. Electroactive bacteria like Shewanella oneidensis enhance the decolorization of azo dyes such as acid orange 7 by up to 97% in bioelectrochemical setups, breaking down chromophoric groups via extracellular electron transfer. Similarly, these systems degrade pharmaceuticals like antibiotics and hormones, with anodic respiration accelerating mineralization and reducing toxicity in contaminated streams. For heavy metals, MFCs enable recovery through cathodic reduction; for example, Cr(VI) is converted to less toxic Cr(III) at efficiencies exceeding 90%, allowing precipitation and reuse of the metal while mitigating environmental leaching.^[55]^[56]^[57]^[58]^[59] Integrated MFC configurations, such as hybrids with membrane bioreactors (MFC-MBR), improve treatment efficacy by combining biological degradation with physical filtration, achieving higher COD and solids removal while minimizing membrane fouling through bioelectric effects. Nutrient recovery is another key advantage, with MFCs promoting the precipitation of struvite (MgNH₄PO₄·6H₂O) from nitrogen- and phosphorus-rich wastewaters; recoveries of up to 80% for phosphorus and 70% for nitrogen have been reported by adjusting pH and magnesium dosing in the cathodic chamber. These systems thus enable resource reclamation alongside treatment.^[60]^[61]^[62] Environmentally, MFCs provide benefits over conventional activated sludge processes by producing significantly less sludge—often 50-70% reduction—due to direct electron transfer minimizing excess biomass growth. They also mitigate greenhouse gas emissions by favoring aerobic respiration over methanogenesis in anaerobic digesters, potentially lowering the carbon footprint of wastewater treatment by up to 90% through avoided methane release and on-site energy recovery.^[63]^[64]^[65] In the 2020s, field trials have demonstrated MFC viability in remote areas, such as stacked soil MFCs in Northeast Brazil powering off-grid water treatment reactors for rural communities without external energy inputs. These deployments underscore MFCs' role in sustainable sanitation for underserved regions.^[66]

Power Generation and Sensing

Microbial fuel cells (MFCs) have been demonstrated as viable power sources for low-energy applications, particularly in remote or autonomous settings where traditional batteries are impractical. Typical power densities for these systems range from 100 to 500 mW/m², sufficient to harvest energy for sensors and small devices.^[67] For instance, sediment-based MFCs, which utilize organic matter in aquatic sediments as fuel, have powered environmental monitoring equipment such as meteorological buoys. In one seminal deployment, a benthic MFC generated 24-36 mW continuously, equivalent to the annual output of multiple alkaline batteries, to operate sensors measuring air and water parameters with real-time telemetry in marine environments.^[68] To address intermittent power needs, MFCs are often integrated with capacitors or supercapacitors, storing generated electricity for burst operations in low-power electronics.^[7] In educational contexts, MFCs serve as accessible tools for demonstrating bioelectrochemistry principles. Low-cost kits, such as the MudWatt system, enable students to construct simple MFCs using soil microbes to generate electricity, powering small LEDs or clocks while exploring concepts in microbiology, electrochemistry, and renewable energy. These kits, priced affordably for classroom use, include anodes, cathodes, vessels, and apps for monitoring bacterial activity and voltage output, fostering hands-on learning without specialized equipment.^[69] MFCs also function as biosensors by leveraging changes in bioelectric output to detect environmental contaminants in real time. The current generated by microbial metabolism decreases upon exposure to toxins, allowing sensitive monitoring of parameters like biochemical oxygen demand (BOD) and heavy metals. For BOD detection, MFC-based sensors achieve rapid assessments with linear responses up to 200 mg/L, outperforming traditional methods in portability and cost.^[15] In toxicity sensing, these devices identify heavy metals such as copper and cadmium at concentrations as low as parts per billion (ppb), where even minor inhibitions in electron transfer cause measurable voltage drops. Similarly, MFCs detect antibiotics and pathogens by observing disruptions in anodic biofilms, with sensitivities reaching ppb levels for compounds like tetracyclines, enabling early warning in water quality monitoring.^[70]^[15] Beyond sensing, MFCs facilitate biorecovery of valuable metals through electrodeposition at the cathode, coupling energy production with resource extraction. In systems treating e-waste leachates, copper ions are reduced and deposited as pure metal, achieving recovery rates over 90% while generating electricity. This process, demonstrated in saline MFCs, recovers copper at rates up to 1.8 kg/m³ per day, with economic viability enhanced by the dual output of power and metals valued at approximately $0.5-2 per kg depending on market conditions.^[71]^[72] Emerging applications in 2025 highlight portable MFCs for challenging environments, including disaster zones and wearables. Compact, soil- or wastewater-fueled designs power sensors in off-grid areas, such as ocean monitoring buoys for emergency response, eliminating battery replacements. Wearable MFCs, integrated into fabrics or patches, harvest energy from sweat or body fluids to drive health trackers, with recent prototypes achieving stable outputs for prolonged use.^[73]^[74]

Challenges and Future Directions

Technical and Scalability Issues

One of the primary technical limitations of microbial fuel cells (MFCs) is their low power density, typically ranging from 0.1 to 1 W/m³ in practical configurations, which remains below targets of 10–100 W/m³ suggested for economic viability in wastewater treatment applications. This shortfall arises mainly from ohmic losses due to high internal resistances in electrodes and electrolytes, as well as mass transport limitations that hinder efficient substrate delivery to biofilms and oxygen supply to cathodes. These factors collectively restrict current generation and overall energy output, making MFCs unsuitable for high-power applications without significant enhancements. Biofilm instability further exacerbates performance issues, as exoelectrogenic bacteria compete with non-electroactive species like methanogens, which divert electrons toward methane production rather than electrode transfer. Electrode fouling by excessive biomass accumulation and extracellular polymeric substances also increases resistance and blocks active sites, leading to rapid degradation. Consequently, MFC longevity can exceed 1–2 years in pilot systems, though regular maintenance is often required to sustain performance beyond 6–12 months, limiting operational reliability in real-world settings. Scalability to large reactors introduces additional engineering hurdles, including uneven flow distribution that causes inconsistent substrate exposure and biofilm development across the system volume. Material costs, particularly for proton exchange membranes (PEMs) at approximately $500–2000/m², drive up overall expenses, with capital costs estimated at $1000–5000/m³ depending on design and scale. These economic barriers, combined with fabrication complexities, have confined most deployments to laboratory or pilot scales under 1 m³. MFCs exhibit sensitivity to environmental factors that disrupt microbial activity; optimal performance occurs at temperatures of 20–35°C, with efficiency dropping sharply outside this range due to slowed metabolic rates or cell lysis. Similarly, pH values between 6 and 8 support robust electrogenesis, while deviations lead to enzyme inactivation or shifts in community composition. Inhibitors such as high salinity (>10 g/L NaCl) suppress exoelectrogens by osmotic stress, further reducing power output and treatment efficacy. From an economic perspective, the levelized cost of electricity (LCOE) for MFCs exceeds $1/kWh in most assessments, rendering them uncompetitive against established renewables like solar and wind, which achieve LCOE below $0.05/kWh. Achieving break-even viability would necessitate overall energy conversion efficiencies greater than 50%, a threshold rarely met due to inherent low Coulombic efficiencies (typically 10–30%) and parasitic losses.

Recent Advances and Commercialization

Recent advances in microbial fuel cell (MFC) technology have focused on material innovations to enhance electron transfer and power output. Nanomaterials, particularly graphene-based electrodes, have demonstrated significant improvements in performance; for example, graphene-modified stainless steel mesh anodes achieved a maximum power density of 2668 mW/m², representing an 18-fold increase over unmodified electrodes.^[75] Similarly, graphene/polypyrrole composites have shown improved power densities compared to plain carbon electrodes. In 2025, electrophoretic deposition of graphene on stainless steel mesh anodes achieved a maximum power density of 5.65 mW/m², an eightfold increase over the 0.70 mW/m² control, highlighting the role of such coatings in promoting biofilm formation and extracellular electron transfer.^[76] Three-dimensional graphene structures with platinum nanoparticles have been explored as freestanding anodes to enhance performance.^[77] Genetic engineering and synthetic biology have emerged as key strategies to optimize microbial performance in MFCs. CRISPR-Cas9 systems have been adapted for electroactive bacteria like Cupriavidus metallidurans, enabling targeted modifications to enhance electron transfer pathways; a 2022 single-plasmid CRISPR-Cas9 delivery method via conjugation or electroporation improved genetic editing efficiency in such strains.^[78] Comparative studies in 2023 showed that genetic optimizations in Geobacter species, including overexpression of outer-membrane cytochromes, increased current densities by up to 50% compared to wild-type strains.^[79] Synthetic biology approaches have stabilized microbial consortia to maintain high electron transfer rates over extended periods. Hybrid MFC systems integrating renewable energy sources and artificial intelligence (AI) have shown promise in pilot-scale applications. Solar-assisted MFCs, combining microbial anodes with photovoltaic cathodes, have enhanced power generation through photo-electrochemical synergy, as detailed in a 2025 review of light-driven configurations.^[80] AI-optimized stacks, using machine learning for real-time parameter adjustment, addressed scalability issues in 2025 innovations, achieving power densities up to 0.6 W/m² in stacked prototypes.^[81] Commercialization efforts include startups like Plant-e, which deploys plant-MFCs in green roofs for urban energy harvesting; a 2025 study integrated these into green infrastructure, generating bioelectricity while managing stormwater.^[82] Emefcy Ltd. has advanced bio-electrochemical treatment systems for wastewater, with deployments emphasizing energy recovery, contributing to the sector's growth.^[83] The global MFC market is projected to reach USD 295.84 million by 2030, driven by wastewater treatment applications.^[84] Looking ahead, MFCs are poised for integration into circular economy models, converting waste streams into valuable resources like electricity and clean water. NASA grants under the 2025 Research Opportunities in Space and Earth Science (ROSES) program support biological engineering in space, potentially extending MFC applications to long-duration missions.^[85] Regulatory and standardization challenges remain a barrier to widespread adoption, necessitating standardized protocols for commercial viability. As of November 2025, pilot projects in Europe and Asia demonstrate integration with existing wastewater facilities, supported by incentives for bioenergy recovery.^[84]

References

[1]
Microbial fuel cells: a comprehensive review for beginners - PMC - NIH
Microbial fuel cells (MFCs) are a solution for energy security, global warming, and wastewater management, performing dual roles of wastewater treatment and ...
[2]
Microbial fuel cells: From fundamentals to applications. A review
BFCs could be defined as devices able to transform chemical to electrical energy via electrochemical reactions involving biochemical pathways and can be divided ...
[3]
Microbial fuel cell: A state-of-the-art and revolutionizing technology ...
This review provides a comprehensive overview of a standard MFC system with emphasis on its working principle, major components, their latest modifications and ...
[4]
Microbial Fuel Cells, A Current Review - MDPI
Microbial fuel cells (MFCs) are devices that can use bacterial metabolism to produce an electrical current from a wide range organic substrates.
[5]
Microbial fuel cells, a renewable energy technology for bio-electricity ...
Microbial fuel cell (MFC) represents an eco-friendly approach to generating electricity while purifying wastewater concurrently, achieving up to 50% chemical ...
[6]
Electricigens in the anode of microbial fuel cells: pure cultures ...
Feb 19, 2019 · The electrons produced during oxidation flow to the cathode through external electric circuit to produce current. In the cathode, electron ...
[7]
Microbial fuel cell as water-energy-environment nexus
... unlike traditional fuel cells, which are based on stable chemical processes. ... catalyst for high-performance asymmetricneutral-alkaline microbial fuel cell.
[8]
Outline of microbial fuel cells technology and their significant ...
In the anodic chamber, microbes degrade the organic substances and produce electrons, protons (H+), and carbon dioxide (CO2). Then, the produced electrons and H ...Missing: definition | Show results with:definition
[9]
A comprehensive review of microbial fuel cells considering materials ...
Feb 15, 2024 · This review article aims to compile comprehensive information about all constituents of the microbial fuel cell, providing practical insights for researchers.Missing: definition | Show results with:definition
[10]
https://www.frontiersin.org/journals/chemical-engineering/articles/10.3389/fceng.2023.1228510/full
[11]
[PDF] Photosynthetic bacterium for long-term space expeditions
In the 1960's the idea of MFC was really picked up and researched further by NASA to be applied to space flight. NASA's idea was to transfer waste into ...Missing: 1970s | Show results with:1970s
[12]
Microbial fuel cells powering robots and beyond - ScienceDirect.com
The first demonstration of electron flow in biological tissue was published in 1791 by the Italian physicist, Luigi Galvani [1]. This study, De viribus ...
[13]
Microbial Fuel Cell-Based Biosensors - PMC - PubMed Central
Jul 23, 2019 · Karube et al. [45] for the first time proposed the use of MFCs as a BOD sensor. Clostridium butyricum bacteria was immobilized on the electrode ...
[14]
Biofilm Biology and Engineering of Geobacter and Shewanella spp ...
Geobacter and Shewanella spp. were discovered in late 1980s as dissimilatory metal-reducing microorganisms that can transfer electrons from cytoplasmic ...Missing: timeline | Show results with:timeline
[15]
Biofilm Biology and Engineering of Geobacter and Shewanella spp ...
Geobacter and Shewanella spp. were discovered in late 1980s as dissimilatory metal-reducing microorganisms that can transfer electrons from cytoplasmic ...Missing: timeline | Show results with:timeline
[16]
Energy by Microbial Fuel Cells: Scientometric global synthesis and ...
The production of electricity using Microbial Fuel Cells (MFCs) is a renewable and sustainable technology that is becoming more efficient [7], [8]. MFCs are ...
[17]
An MEC-MFC-Coupled System for Biohydrogen Production from ...
Microbial fuel cells (MFCs) are devices that use bacteria as the catalysts to oxidize organic and inorganic matter and generate current, whereas microbial ...
[18]
Microbial Fuel Cells: Making Electricity from Sewage
Oct 15, 2015 · Cambrian Innovation, founded in 2006, sells microbial fuel cells for ... [In 2017, Emefcy merged with RWF Water Solutions to form Fluence.].
[19]
Resources - Cambrian Innovation
Cambrian, a Leader in Wastewater Treatment, Water Reuse and Energy Recovery-As-A-Service, is Acquired by, and Receives $200 Million Growth Equity Commitment ...Missing: cells | Show results with:cells
[20]
Experimental Proof of Principle of 3D-Printed Microfluidic Benthic ...
Microbial fuel cells (MFCs) represent a method of generating electrical power via microbial processes by the connection of an anaerobic anode to an aerobic ...Missing: 2020s | Show results with:2020s
[21]
New horizons in microbial fuel cell technology: applications ...
Jul 18, 2025 · Microbial fuel cells (MFCs) have emerged as a promising technology to convert biomass and organic waste into electricity, offering an eco-friendly and ...
[22]
Full article: A state of the art review on electron transfer mechanisms ...
Dec 4, 2020 · This review discusses electron transfer mechanisms in MFCs and important factors affecting the performance of MFC.
[23]
Cytochromes in Extracellular Electron Transfer in Geobacter
Apr 27, 2021 · Extracellular electron transfer (EET) is an important biological process in microbial physiology as found in dissimilatory metal ...
[24]
Effect of Glucose and Methylene Blue in Microbial Fuel Cells Using ...
Dec 4, 2023 · Electron transfer can also be achieved by using exogenous mediators ... electron transfer mediated by methylene blue in a microbial fuel ...
[25]
Electricity generation from acetate and glucose by sedimentary ...
Microbial-anode fuel cells (MAFCs) with high electron recovery (>50%) from acetate and glucose have been constructed in this study.
[26]
Important Factors Influencing Microbial Fuel Cell Performance
Variables such as bacterial type, pH, temperature, and substrate type also impact performance [31][32] [33] , with and biofilm-electrode interactions ...
[27]
Microbial Fuel Cells: Methodology and Technology†
### Summary of Electricity Generation Process in Microbial Fuel Cells (MFCs)
[28]
https://www.sciencedirect.com/science/article/pii/S0378775318306013
[29]
Microbial fuel-cells | Applied Biochemistry and Biotechnology
Microbial fuel cells containingProteus vulgaris and oxidation-reduction (“redox”) mediators were investigated.
[30]
Shewanella secretes flavins that mediate extracellular electron transfer
Electron transfer by two strains of Shewanella to these surfaces is mediated by flavins, which are actively secreted by the cells.
[31]
Energy harvesting from plants using hybrid microbial fuel cells
Jan 31, 2024 · One advantage of plant based MFCs is that they operate in the absence of a PEM separator, because the soil supporting the plants growth also ...
[32]
Plant microbial fuel cells: A comprehensive review of influential ...
This review delves into the multifaceted landscape of plant microbial fuel cells (PMFCs), investigating the affecting factors, configurations, applications, ...
[33]
Microbial Fuel Cell Technology as a New Strategy for Sustainable ...
This article reviews the current state of the art in the field of application of MFC technology to various soil-based ecosystems.
[34]
Algae-assisted microbial fuel cells: A practical overview
By incorporating photosynthetic organisms such as algae in the cathode instead, we get electron acceptors, biomass, as well as dissolved oxygen from ...Missing: phototrophic | Show results with:phototrophic
[35]
A revolving algae biofilm based photosynthetic microbial fuel cell for ...
Photosynthetic microbial fuel cell (PMFC) based on algal cathode can integrate of wastewater treatment with microalgal biomass production.Missing: phototrophic | Show results with:phototrophic
[36]
Oxygenic phototrophic biofilms for improved cathode performance in ...
It is shown here that the growth of oxygenic phototrophs as a biofilm, increases the current output by two fold.Missing: biofilm | Show results with:biofilm
[37]
New insights into microbial electrolysis cells (MEC) and microbial ...
Jan 1, 2024 · Microbial fuel cell (MFC) and microbial electrolysis cell (MEC) are the notable systems used for hydrogen production from organic wastes and ...
[38]
Microbial electrolysis: a promising approach for treatment and ...
Mar 17, 2022 · The microbial electrolysis cell (MEC) is one of the most efficient technologies for waste-to-product conversion that uses electrochemically active bacteria.
[39]
Microbial Fuel Cells and Microbial Electrolysis Cells for the ... - MDPI
Two promising bioelectrochemical systems that use microorganisms to produce hydrogen and energy from organic matter are microbial fuel cells (MFCs) and ...
[40]
Tubular ceramic performance as separator for microbial fuel cell
Aug 9, 2025 · Commercially produced ceramic structures have been proven to reduce Chemical Oxygen Demand up to 92% and allow high power output. It is also ...
[41]
Ceramic Microbial Fuel Cells Stack: power generation in standard ...
Feb 19, 2018 · In this work, a microbial fuel cell (MFC) stack containing 28 ceramic MFCs was tested in both standard and supercapacitive modes.
[42]
A recent development of low-cost membranes for microbial fuel cell ...
This review focuses on membranes that are low in cost and have higher performance when compared with Nafion membranes.
[43]
Sediment microbial fuel cells capable of powering outdoor ...
In this study, Sediment Microbial Fuel Cells (SMFCs) prototypes have been developed to operate under open-air conditions and power sensors for environmental ...
[44]
Sediment microbial fuel cells as a barrier to sulfide accumulation ...
Aug 4, 2020 · In conclusion, we have demonstrated that SMFCs have the potential to remove sulfide from sediments at a scale that could benefit the benthic ...
[45]
Benthic microbial fuel cell systems for marine applications
Feb 28, 2022 · Sediment microbial fuel cells (SMFCs) provide a promising sustainable technological platform that has been proposed for multiple applications, ...
[46]
Series Assembly of Microbial Desalination Cells Containing Stacked ...
Jun 15, 2011 · These results demonstrate that stacked MDCs can be used for efficient desalination of seawater while at the same time achieving power densities comparable to ...
[47]
Performance of microbial desalination cells with different cathode ...
May 9, 2025 · Unlike traditional microbial fuel cells (MFCs), MDCs introduce an additional desalination chamber between the anode and cathode, forming a three ...
[48]
Long-Term Performance of Liter-Scale Microbial Fuel Cells Treating ...
Mar 21, 2013 · In general, both MFCs achieved similar COD removal efficiency of 65–70% or COD removal rate of ∼0.40 kg of TCOD m–3 day–1 or ∼0.22 kg of SCOD m– ...
[49]
Optimization of Microbial Fuel Cell Performance for Fertilizer ...
Sep 27, 2025 · Results demonstrate that MFCs can achieve significant treatment efficiencies (COD > 80%, ammonia > 70%) while generating stable power outputs up ...<|separator|>
[50]
Pilot-Scale MFCs | Microbial fuel cells & METs - Sites at Penn State
The effect of flow modes and electrode combinations on the performance of a multiple module microbial fuel cell installed at wastewater treatment plant.
[51]
Impact of reactor configuration on pilot-scale microbial fuel cell ...
Oct 15, 2022 · The authors acknowledge funding by Penn State. ... An integrated 45 L pilot microbial fuel cell system at a full-scale wastewater treatment plant.
[52]
Bioelectrochemical reduction of an azo dye by a Shewanella ...
In this study, we investigated the decolorization of a model azo dye, acid orange 7 (AO7) in a biocathode inoculated with a model electrogenic microorganism ...Missing: pharmaceuticals | Show results with:pharmaceuticals
[53]
Degradation of Azo Dye (Acid Orange 7) in a Microbial Fuel Cell
System 1 was dye in the anode with Shewanella oneidensis MR1, and laccase enzyme in the cathode, subsequently to be referred to as “MFC Dye Anode.” System 2 was ...
[54]
Microbe mediated remediation of dyes, explosive waste and ...
Dec 18, 2021 · This review covers the research carried out in the field of bioremediation of dyes, PAHs, pharmaceuticals, explosive waste, and pesticides in the last decade
[55]
Biological Chromium(VI) Reduction in the Cathode of a Microbial ...
In this study, we demonstrated biological Cr(VI) reduction in the cathode of a MFC and identified putative Cr(VI) reducing microorganisms. The MFC was ...
[56]
The Potential of Microbial Fuel Cells for Remediation of Heavy ... - NIH
Dec 13, 2019 · One typical Cr(VI) reduction in MFC is presented in Equation (3) and the low pH could positively affect the metal's recovery. Higher H+ serving ...
[57]
A Perspective Review on Microbial Fuel Cells in Treatment and ...
Jan 12, 2023 · This review discusses the developments in MFC technologies, including improvements to their structural architecture, integration with different novel ...
[58]
Nutrient removal and recovery from wastewater by microbial fuel cell ...
Jun 24, 2022 · Microbial fuel cell (MFC) is a green innovative technology that can be employed for nutrient removal/recovery as well as for energy production from wastewater.
[59]
Nutrient removal and recovery from wastewater by microbial fuel cell ...
Jul 12, 2025 · Recovery of nutrients as struvite or cattiite by precipitation, as microalgal biomass and as ammonium salts are common. This review shows that ...
[60]
A Critical Review on Microbial Fuel Cells Technology
Aug 27, 2021 · This paper provides critical insights about the MFC processes, application of MFCs in wastewater treatment, and challenges of MFC technology. 2.2. Microbial Fuel Cell · 2.1. Microbial Fuel Cell... · 2.4. ExoelectrogensMissing: seminal | Show results with:seminal
[61]
Towards sustainable wastewater treatment by using microbial fuel ...
Nov 28, 2013 · MFCs may also have a low carbon footprint, arising from less fossil-related CO2 production as a result of low energy consumption as well as ...
[62]
Greenhouse gas emissions in a microbial fuel cell-assisted ...
Aug 6, 2025 · Although sufficient oxygen can reduce GHG emissions by serving as an electron acceptor, elevated bacterial activity may enhance GHG production ...
[63]
Development of a functional stack of soil microbial fuel cells to ...
Aug 9, 2025 · Development of a functional stack of soil microbial fuel cells to power a water treatment reactor: From the lab to field trials in North East ...Missing: 2020s | Show results with:2020s
[64]
Assessment of Microbial Fuel Cell Configurations and Power Densities
Jul 30, 2015 · In general, power densities might be expected to increase when using a higher-strength wastewater, but electrode packing density is more ...Missing: mediated | Show results with:mediated
[65]
https://www.sciencedirect.com/science/article/abs/pii/S0013935125017566
[66]
MudWatt Microbial Fuel Cell Kit
In stock 1-day deliveryThe MudWatt is the perfect educational kit for classrooms and hobbyists since it incorporates a wide range of scientific topics.
[67]
Effect of external resistance on the sensitivity of microbial fuel cell ...
The relatively poor sensitivity is the main bottleneck restricting the application of microbial fuel cell biosensor (MFC-biosensor) for toxicity monitoring.Missing: pathogens | Show results with:pathogens
[68]
Improving Scalability of copper recovery in saline microbial fuel cells ...
Microbial fuel cells (MFC) are emerging energy-efficient systems for copper (Cu) electrowinning from waste streams by coupling it with anodic oxidation of ...
[69]
High rate copper and energy recovery in microbial fuel cells - PMC
Copper recovery as a cathode reaction can potentially lead to high current densities due to its low overpotential. It is therefore, a suitable cathode reaction ...
[70]
$$7.8M Award Aims to Revolutionize Renewable Energy…
Jan 2, 2025 · The $7.8M award is for a microbial fuel cell using ocean microbes to power ocean sensing devices, eliminating the need for batteries and cables.<|separator|>
[71]
A review on recent advancements in wearable microbial fuel cells
This review article focuses on how wearable MFCs are changing the energy landscape and ushering in a new era of inventive electronics.Missing: portable disaster zones
[72]
Applications of Nanomaterials in Microbial Fuel Cells: A Review - NIH
Nov 2, 2022 · [60] employed graphene to improve stainless steel mesh and achieved a maximum power density of 2668 m W/m2, which was 18 times higher than the ...
[73]
Microbial Fuel Cell Performance Boost through the Use of Graphene ...
Jan 4, 2023 · Authors obtained 36 times more electricity generation than that of pure carbon paper as anode (maximum power density of 5 mW/m3 and electrode ...
[74]
[PDF] Performance Enhancement of Microbial Fuel Cells Using Graphene ...
Aug 8, 2025 · Experimental results show that the EPD-coated graphene anode significantly boosts power output, raising the maximum power density from. 0.70 mW/ ...
[75]
Three-dimensional graphene/Pt nanoparticle composites as ...
Nov 13, 2015 · Unfortunately, the power density of the MFCs at present is significantly lower than the theoretical value because of technical limitations ...
[76]
Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR ...
Oct 24, 2022 · A single-plasmid CRISPR-Cas9 system that can be delivered by both conjugation and electroporation was developed, and its efficiency was ...
[77]
Microbe–Anode Interactions: Comparing the impact of genetic and ...
Feb 18, 2023 · Genetic optimizations for Geobacter species. Though most research groups rely on genetic modification of S. oneidensis, there are also studies ...
[78]
A Review of Recent Advances in Microbial Fuel Cells - ResearchGate
Oct 13, 2025 · This review presents the latest progress in microbial community selection and genetic engineering techniques for enhancing microbial electricity production.<|separator|>
[79]
A brief review on factors affecting the performance of microbial fuel ...
Jul 28, 2025 · Renewed interest in MFCs began in 1931, when Barnet Cohen successfully generated 35 V by connecting multiple cells in series [32]. Despite ...Missing: Barnett | Show results with:Barnett
[80]
https://link.springer.com/article/10.1007/s43621-025-01619-6
[81]
Integrating plant microbial fuel cells into green infrastructure
Sep 29, 2025 · This study investigates bioelectricity generation using plant microbial fuel cells (PMFCs) integrated into a GI system constructed with recycled ...
[82]
Microbial Fuel Cell Market Companies
Top Players in Microbial Fuel Cell Market · Emefcy Ltd. · Cambrian Innovation Inc. · Microbial Robotics · Protonex Technology Corporation · Prongineer · ElectroChem ...
[83]
Microbial Fuel Cell Market Size, Share & 2030 Trends Report
Sep 23, 2025 · The Microbial Fuel Cell Market size is estimated at USD 230.92 million in 2025, and is expected to reach USD 295.84 million by 2030, at a CAGR ...
[84]
Research Opportunities in Space and Earth Science (ROSES)-2025 ...
Jul 9, 2025 · Team members from government agencies other than NASA are welcome at no cost (as unfunded Co-Is or collaborators). Awards range from under $100K ...
[85]
Recent Applications, Challenges, and Future Prospects of Microbial ...
Microbial fuel cells (MFCs) are used for wastewater treatment, bioelectricity, and more. Challenges include system complexity and low power output. Future ...