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Laboratory automation

Laboratory automation encompasses the integration of robotic systems, software, and hardware to execute laboratory processes with minimal human intervention, including specimen handling, analysis, and data management, thereby enhancing efficiency and reducing errors in clinical, research, and industrial settings.^[1]^[2] This multidisciplinary field employs technologies such as conveyor tracks, liquid handlers, machine vision, and informatics tools to automate workflows from pre-analytic preparation to post-analytic reporting.^[1]^[3] The origins of laboratory automation trace back to the late 19th century, with the earliest documented U.S. chemical literature reference in 1875 describing an unattended device for washing filtrates.^[4] Significant advancements occurred in the mid-20th century; in 1956, Leonard Skeggs developed the AutoAnalyzer, the first fully automated continuous flow system for measuring analytes like urea, glucose, and calcium in clinical chemistry.^[5] The 1960s and 1970s saw innovations such as discrete analyzers and centrifugal systems, while the 1980s introduced robotics through companies like Zymark, enabling high-throughput screening in pharmaceuticals.^[5]^[6] By the 1990s, total laboratory automation (TLA) emerged, connecting pre-analytic, analytic, and post-analytic phases via track systems, with widespread adoption in Japan, Europe, and North America by the early 2000s.^[1]^[3] Key components of modern laboratory automation systems include automated centrifuges, sorters, immunoassay analyzers, and robotic arms for liquid handling, often scaled across levels from semi-automated tools (e.g., electronic pipettes) to fully automated cloud labs.^[1]^[7] In clinical microbiology, these systems automate inoculation, incubation, and imaging of specimens like agar plates and blood cultures.^[2] TLA platforms, such as the Atellica Solution, further integrate reagent delivery and data handling for seamless operation.^[3] Laboratory automation yields substantial benefits, including a reduction in human errors by over 70%, shortened turnaround times (e.g., up to 50% in clinical labs), and increased productivity—such as 1.4-fold in chemistry and 3.7-fold in serology per worker.^[1]^[3] It enhances reproducibility, minimizes contamination, and improves patient safety by standardizing processes, while allowing staff to shift from repetitive tasks to quality control and complex analyses.^[7]^[2] In research settings, it accelerates high-throughput experiments and facilitates bench-to-bedside translation.^[7] Despite these advantages, challenges persist, including high initial costs, equipment obsolescence, and potential limitations in flexibility for novel protocols, though ongoing innovations in IoT, microfluidics, and AI continue to address these issues.^[7] Applications span clinical diagnostics, life sciences, and drug discovery, with TLA proving particularly vital amid workforce shortages and rising testing demands.^[3]

Fundamentals

Definition and Scope

Laboratory automation refers to the integration of robotic systems, software, and interconnected workflows to execute repetitive laboratory tasks—such as sample preparation, chemical analysis, and data management—with reduced human involvement.^[8] This approach encompasses hardware like liquid handlers and robotic arms alongside computational tools for process control, enabling consistent execution of protocols that would otherwise rely on manual operations.^[9] The scope of laboratory automation extends across wet laboratories, where physical manipulations like pipetting, mixing, and centrifugation occur, and dry laboratories focused on computational tasks such as data processing and simulation modeling.^[7] Unlike industrial automation, which prioritizes high-volume mass production in manufacturing settings, laboratory automation emphasizes precision for variable scientific experiments, scalability for small-to-medium batch sizes, and seamless integration with research-specific protocols to support hypothesis-driven work.^[10] Core principles include enhancing reproducibility by standardizing procedures, increasing throughput to handle greater sample volumes efficiently, and minimizing errors through mechanized precision rather than human variability.^[11] Automation in laboratories operates across varying levels, from semi-automated systems involving human-robot collaboration for setup and oversight, to fully automated end-to-end processes that manage entire workflows autonomously.^[12] Key concepts distinguish total laboratory automation (TLA), which automates pre-analytical, analytical, and post-analytical stages in an integrated track-based system, from task-specific automation that targets isolated functions like individual assays.^[13] For instance, high-throughput screening (HTS) exemplifies an automated process where robotics rapidly test thousands of compounds against biological targets to identify potential hits, accelerating discovery without exhaustive manual screening.^[14]

Importance in Modern Laboratories

Laboratory automation plays a pivotal role in modern laboratories by significantly enhancing operational efficiency and reliability to meet escalating demands for high-volume, precise testing. One of its primary benefits is the substantial increase in throughput, allowing labs to process thousands of samples daily through streamlined workflows that minimize bottlenecks.^[15] This capability enables continuous 24/7 operations, optimizing resource utilization without constant human oversight.^[16] Additionally, automation reduces human error rates dramatically; for instance, in blood group and antibody testing, it can decrease error opportunities by 90-98% compared to manual methods, particularly in pipetting tasks prone to variability.^[17] By handling repetitive procedures, automated systems free scientists to focus on higher-level analysis and innovation, thereby boosting overall productivity.^[3] Economically, laboratory automation delivers long-term cost savings that often yield a strong return on investment (ROI), with payback periods as short as a few years through reduced labor and operational expenses. For example, it minimizes reagent waste by enabling smaller reaction volumes and precise dispensing, lowering consumable costs per test. Furthermore, automated processes support compliance with quality standards by standardizing operations and reducing variability in pre-analytical phases. These efficiencies can cut total hospital expenses over time while maintaining high standards of quality management.^[18] Beyond immediate lab operations, automation has broader implications for advancing scientific discovery, particularly in high-complexity fields like genomics and proteomics, where it accelerates workflows by enabling high-throughput data generation and reproducible experiments.^[19] It also enhances safety by limiting personnel exposure to hazardous materials through robotic handling of toxic substances, thereby reducing health risks in routine procedures.^[20] Typical efficiency gains include at least 5-10 times faster processing in automated setups versus manual ones, as seen in sample preparation and analysis scenarios, allowing labs to scale operations without proportional increases in staff or time.^[21]

History

Origins and Early Developments

The origins of laboratory automation trace back to the late 19th century, when chemists began developing rudimentary mechanical devices to address the tedium of repetitive manual tasks in analytical processes. The earliest documented instance in U.S. chemical literature appeared in 1875, with Thaddeus M. Stevens describing an unattended filtrate-washing apparatus that utilized a lamp chimney and steam jet to generate vacuum for controlled washing of filter residues.^[22] This innovation marked an initial shift toward mechanization, allowing basic operations like filtration and washing to proceed without constant supervision, thereby improving efficiency in chemical purification workflows.^[22] In the late 19th and early 20th centuries, further mechanical aids emerged, inspired by broader industrial automation trends such as assembly lines and powered machinery. For instance, in 1894, E.R. Squibb introduced the "automatic zero burette," featuring an inverted siphon mechanism that reset the zero point after each titration, enabling repeated measurements of liquids with varying densities without manual refilling.^[22] Similarly, fraction collectors for distillation processes began incorporating siphons and simple timers by the 1930s, automating the sequential collection of distillate fractions to support purification in resource-constrained industrial settings.^[22] These developments drew from industrial precedents, like Henry Ford's 1913 assembly line, which emphasized sequential mechanization and influenced laboratory tools by highlighting the benefits of reducing human error in repetitive tasks.^[22] Pre-1950s milestones highlighted the growing integration of photoelectric and conductivity-based technologies in analytical chemistry, driven by needs in industrial quality control and wartime exigencies. In the 1920s, photoelectric analyzers, such as early colorimeters developed by institutions like New York University and Eastman Kodak, enabled automated detection of color changes in titrations, including those for blood sugar levels via reduction methods.^[22] By the 1930s and 1940s, wartime labor shortages spurred further advancements, such as Shell Oil's automated mercaptan titrator, which streamlined chemical analysis for manufacturing quality assurance.^[22] Key figures like H.M. Partridge and Ralph H. Muller championed these photoelectric innovations, fostering a conceptual shift among early adopters in analytical chemistry toward semi-automated systems that handled high-volume, repetitive assays in industrial laboratories.^[22]

Evolution in the 20th and 21st Centuries

The evolution of laboratory automation in the 20th century began with the introduction of the AutoAnalyzer by Technicon Corporation in 1957, marking a pivotal milestone in continuous-flow clinical chemistry analysis. Invented by biochemist Leonard Skeggs, this system automated the processing of multiple samples simultaneously, enabling up to 40 tests per hour and significantly reducing manual labor in diagnostic labs.^[23] This innovation laid the groundwork for scalable, high-volume testing, transitioning laboratories from labor-intensive manual methods to mechanized workflows.^[23] In the 1970s and 1980s, laboratory automation advanced with the integration of industrial robots and microprocessor-controlled systems, enhancing precision in sample handling and analysis. The introduction of robots in the early 1970s facilitated automated liquid handling tasks, while robotic pipettors emerged in the late 1970s and early 1980s, allowing for programmable and repeatable pipetting operations.^[8] By the mid-1980s, microprocessor-driven robots had become commonplace, enabling sophisticated control over electromechanical components for tasks like mixing and dispensing, which improved accuracy and throughput in research settings. The 1990s and 2000s saw rapid expansion driven by high-throughput screening (HTS) in the pharmaceutical industry and the integration of Laboratory Information Management Systems (LIMS). HTS, which originated in natural products screening in the late 1980s and scaled up in the 1990s, allowed pharma companies to test thousands of compounds daily using automated robotic platforms, accelerating drug discovery; by 1992, it contributed hits to about 40% of discovery portfolios at firms like Pfizer.^[24] LIMS, emerging commercially in the late 1980s and gaining prominence around 1990, digitized sample tracking and data management, streamlining workflows across labs.^[25] During the Human Genome Project (1990-2003), automation in DNA sequencing advanced with the adoption of fluorescent dye-labeled Sanger methods in the 1990s, enabling faster and more reliable genomic analysis in research.^[26] From the 2010s onward, laboratory automation incorporated collaborative robots (cobots) and AI-assisted workflows, fostering safer and more adaptive systems. Cobots, designed for human-robot collaboration without extensive safety barriers, began entering labs in the mid-2010s, handling repetitive tasks like sample preparation while allowing technicians to oversee complex processes.^[27] AI integration enhanced workflow optimization, such as predictive error detection and real-time data analysis, further boosting efficiency in diverse applications.^[7] This period also witnessed substantial market growth, from approximately $3.8 billion in 2015 to $6.4 billion as of 2025, reflecting broader industry adoption.^[28]

Technologies

Hardware Systems

Laboratory automation relies on a variety of hardware systems designed to perform precise, repetitive tasks with minimal human intervention, enhancing efficiency and reproducibility in scientific workflows. These systems encompass mechanical components that handle sample manipulation, transport, and processing, often integrated into modular frameworks to accommodate diverse laboratory needs. Core hardware includes liquid handling systems and robotic arms, while supporting elements such as transport mechanisms and storage units enable seamless operation. Liquid handling systems form the backbone of many automated laboratory processes, utilizing robotic pipettors to dispense and transfer precise volumes of liquids into multi-well plates, such as 96-well formats commonly used in assays. These systems employ motorized syringes or peristaltic pumps attached to robotic arms, achieving high precision, typically with coefficients of variation below 5% for volumes in the 10–100 μL range.^[29] For instance, platforms like the Biomek series from Beckman Coulter integrate multi-channel heads for parallel processing, supporting high-throughput applications while minimizing contamination risks through disposable tips.^[30] Robotic arms, often 6-axis manipulators, facilitate sample transfer by gripping and moving containers like tubes or plates between stations, with payload capacities typically ranging from 1–5 kg and positional accuracies of approximately 0.2 mm. Examples include the Nucleus robotic arms from HighRes Biosolutions, which enable flexible navigation in confined lab spaces for tasks such as plate stacking or instrument loading.^[31]^[32] Supporting hardware extends functionality through transport and storage solutions. Conveyor belts and track systems, such as rail-based architectures, automate sample movement across workcells, allowing containers to travel at speeds up to 1 m/s while maintaining orientation for downstream processing. Automated storage and retrieval systems (AS/RS) for reagents and samples use robotic cranes or shuttles to access inventory in climate-controlled environments, with capacities for thousands of tubes or plates stored at temperatures from ambient to -80°C. Programmable centrifuges and shakers integrate directly into these setups, featuring variable speed controls (e.g., 100–10,000 rpm for centrifuges) and orbital shaking up to 3000 rpm to standardize incubation conditions.^[32]^[33] Integration of these components emphasizes modular designs that scale from compact benchtop units processing 100 samples per hour to full walk-away systems handling up to 1000 samples per hour, allowing laboratories to expand without complete overhauls. Sensors, including barcode scanners for sample identification and machine vision systems for position verification, provide real-time feedback to ensure error-free operations, with detection accuracies exceeding 99%. Emerging hardware includes AI-enhanced machine vision for improved anomaly detection in automated workflows.^[7] Materials like autoclavable plastics (e.g., polypropylene for tips and trays) and stainless steel for frames maintain sterility and durability, supporting compliance with relevant standards such as ISO 13485 for medical devices and ISO/IWA 15 for liquid handling performance.^[32]^[34]^[35] This hardware architecture, as analyzed in flexible automation studies, promotes reconfigurability through mechatronic building blocks like interchangeable joints and actuators.

Software and Control

Laboratory automation relies heavily on specialized software to manage workflows, integrate devices, and ensure data integrity. Laboratory Information Management Systems (LIMS) serve as central platforms for tracking samples and data throughout the laboratory process. Key features include sample tracking from collection to storage, audit trails that log all changes for compliance with regulations like 21 CFR Part 11, and inventory management to monitor reagents, equipment, and supplies in real-time. These capabilities enable laboratories to maintain traceability and reduce manual errors in high-volume operations.^[36] Electronic Lab Notebooks (ELN) complement LIMS by facilitating the documentation and scripting of experimental protocols. ELNs allow researchers to create standardized templates for protocols, standard operating procedures (SOPs), and workflows, which can include automated data entry from instruments via APIs. This scripting functionality supports the design and execution of repeatable experiments, with features for annotating raw data. ELNs can integrate with other systems to enhance reproducibility in research settings.^[37] Control systems in laboratory automation use middleware to integrate diverse hardware devices, ensuring seamless communication and interoperability. Middleware platforms, such as those adhering to the Standardization in Laboratory Automation (SiLA) protocol, enable device discovery, command execution, and data exchange across instruments like pipettors and analyzers. The SiLA 2 standard, for instance, provides a framework for workflow orchestration using Ethernet/TCP IP, allowing rapid integration without custom coding for each device. Similarly, the Society for Laboratory Automation and Screening (SLAS) promotes standards like OPC UA Laboratory Automation Device Standard (LADS) for plug-and-play connectivity in automated workflows. Scripting languages further enhance control; Python, with libraries like PyOpticon, is widely used for custom automation scripts due to its flexibility in handling data acquisition and instrument commands. LabVIEW, a graphical programming environment from National Instruments, excels in visual control system design, enabling engineers to build intuitive interfaces for real-time device monitoring and adjustment.^[38]^[39]^[40] Advanced software features address operational efficiency and reliability. Real-time analytics dashboards, integrated into LIMS and ELNs, visualize key performance indicators such as throughput, error rates, and resource utilization, allowing operators to monitor workflows dynamically. Error-handling algorithms, often based on if-then rules, detect anomalies like failed pipetting and trigger corrective actions, such as retrying operations or alerting personnel, thereby minimizing downtime. Cloud-based platforms enable remote monitoring, providing access to live data streams, asset management, and troubleshooting tools from anywhere, which supports distributed teams and predictive maintenance.^[41]^[42]^[43] Standardization protocols ensure the robustness and interoperability of these systems. The ASTM E1578 standard guides the validation of laboratory informatics tools, including LIMS, by outlining requirements for specification, implementation, and ongoing verification to meet operational and regulatory needs across the system lifecycle. For instrument communication, XML-based formats like the Analytical Information Markup Language (AnIML) standardize data exchange, facilitating the transfer of results and metadata between devices and software in a vendor-neutral manner. These efforts promote consistent validation and data flow, essential for scalable automation.^[44]^[45]

Applications

In Clinical Diagnostics

Laboratory automation plays a pivotal role in clinical diagnostics by enhancing the efficiency, accuracy, and scalability of patient sample processing in healthcare settings, where high volumes of tests are required for timely diagnosis and treatment. Automated systems streamline the handling of diverse specimens, such as blood and tissue samples, reducing human error and enabling laboratories to manage increased workloads without proportional staff increases. This is particularly crucial in hospital and reference labs, where automation supports the transition from manual to integrated workflows, ensuring reliable results for conditions ranging from routine blood disorders to infectious diseases.^[46] Primary applications include automated hematology analyzers, which perform complete blood counts and differential white blood cell analyses using technologies like flow cytometry and impedance measurements to provide five- to seven-part differentials with high precision. These analyzers process thousands of samples daily, offering rapid results essential for diagnosing anemias, infections, and leukemias. In molecular diagnostics, integrated high-throughput systems have been instrumental for COVID-19 PCR testing; for instance, platforms like the Roche cobas 6800/8800 enable processing of up to 96 samples per run in about 3 hours, with the cobas 8800 supporting up to 1,056 tests in an 8-hour shift and peak capacities exceeding 10,000 tests per day in large clinical labs with multiple systems during surges. Such systems automate nucleic acid extraction, amplification, and detection, minimizing contamination risks and accelerating outbreak responses. As of 2025, integrations of artificial intelligence (AI) and the Internet of Medical Things (IoMT) further optimize these workflows by enabling predictive analytics and equipment maintenance.^[47]^[48]^[49] Workflow automation spans pre-analytical and post-analytical phases to optimize the total testing process. In pre-analytical stages, robotic sorters and decappers handle sample sorting, labeling, and centrifugation, ensuring traceability and significantly reducing errors like mislabeling in high-volume settings. Post-analytical automation facilitates result validation, archiving, and seamless integration with electronic health records (EHRs) via middleware, allowing automated transmission of data to clinicians for immediate decision-making. This integration enhances interoperability, as seen in systems that flag critical values and route reports directly to EHR platforms like Epic or Cerner. Additionally, automation extends to point-of-care testing, where compact devices perform end-to-end blood analysis— from venipuncture to reporting—in minutes, supporting bedside diagnostics in emergency departments.^[46]^[50]^[51]^[52] Case studies demonstrate significant impacts on operational efficiency; for example, implementation of total laboratory automation at Geisinger Medical Center reduced discrete specimen handling steps by 86%, achieving consistent turnaround times for routine tests of less than 45 minutes. In another instance, Zhongshan People's Hospital integrated automation with informatics, cutting overall turnaround times by 77% while boosting staff satisfaction to 85%. These improvements not only accelerate patient care but also allow reallocation of personnel to complex tasks. Regulatory compliance is ensured through adherence to Clinical Laboratory Improvement Amendments (CLIA) standards, which mandate proficiency testing, quality control, and validation for automated systems to guarantee diagnostic accuracy across pre-, analytical, and post-analytical phases. Labs must verify that automation maintains analytical performance equivalent to manual methods, with FDA categorization of devices under CLIA complexity levels (waived, moderate, or high) guiding implementation.^[53]^[54]^[55]^[56]

In Research and Development

Laboratory automation plays a pivotal role in research and development (R&D) by enabling scientists to conduct complex experiments at unprecedented scales and speeds, particularly in fields like drug discovery and genomics. In high-throughput drug screening (HTS), automated systems facilitate the rapid testing of vast compound libraries against biological targets, allowing researchers to identify potential therapeutic candidates efficiently. For instance, platforms can screen up to 100,000 compounds in a single run, dramatically accelerating the initial phases of drug discovery where manual processes would be prohibitively slow.^[57] These systems integrate robotic liquid handlers, plate readers, and data analysis software to minimize human intervention, ensuring consistent assay conditions across thousands of samples.^[14] Another key application is in automated CRISPR gene editing workflows, which streamline the design, execution, and analysis of genome modifications for functional studies. High-throughput platforms using CRISPR/Cas9 can process thousands of samples per week, enabling parallel editing of multiple genes to explore genetic interactions or validate hypotheses in model organisms.^[58] In genomics laboratories, robotic sequencers automate the entire pipeline from sample preparation to variant calling, processing hundreds of DNA samples daily with reduced error rates compared to manual methods. This automation supports large-scale population studies or personalized medicine research by generating high-quality sequence data for downstream bioinformatics analysis.^[59] Similarly, in biotechnology, automated systems for protein crystallization have revolutionized structural biology; robotic dispensers set up thousands of crystallization trials overnight, optimizing conditions for X-ray crystallography and accelerating the determination of protein structures essential for drug design.^[60] The advantages of laboratory automation in R&D extend to enabling hypothesis testing at scale and supporting iterative experimentation with precise replication. Automated workflows allow researchers to run multiple variations of an experiment simultaneously, collecting reproducible data that strengthens statistical power and facilitates the refinement of scientific models.^[61] In pharmaceutical R&D, companies like Beckman Coulter provide integrated HTS platforms, such as the Biomek series, which combine liquid handling robotics with modular assay tools to handle diverse screening formats, from cell-based assays to biochemical tests.^[62] In academic settings, automation is increasingly adopted in synthetic biology, where open platforms enable the assembly and testing of genetic circuits at high throughput, fostering innovation in bioengineering applications like biofuel production or novel therapeutics. As of 2025, AI-driven self-driving laboratories are emerging to further accelerate processes like enzyme optimization in synthetic biology.^[63]^[64] Overall, these tools shift the focus from routine tasks to creative problem-solving, enhancing the pace and reliability of scientific discovery.^[65]

Challenges and Advancements

Implementation Challenges

One major technical challenge in implementing laboratory automation is the integration of new systems with existing legacy equipment, often complicated by incompatibilities in data storage formats and module interfaces.^[66] Vendor lock-in exacerbates this issue, as proprietary protocols from different manufacturers limit flexibility and increase dependency on specific suppliers for upgrades or repairs.^[67] Additionally, maintenance requirements can result in significant operational downtime, with system failures posing risks of prolonged interruptions that disrupt workflows unless mitigated by robust vendor support and onsite diagnostics.^[68] Human factors present another barrier, particularly the need for comprehensive training of operators to handle complex automated systems effectively. Manufacturer-provided on-site training is typically required, along with the designation of "super users" for troubleshooting, to minimize errors during the initial adoption phase.^[69] Resistance to change among staff accustomed to manual workflows is common, stemming from concerns over job displacement or increased complexity, which can hinder smooth transitions and necessitate change management strategies.^[70] These human-related hurdles can undermine the potential benefits of automation, such as reduced human error in sample handling. Operational challenges include scalability across laboratories of varying sizes and volumes, where systems must be configured to match test demands—often requiring multiple instruments for high-throughput environments—while accommodating diverse tube sizes and specimen types through specialized programming.^[68] Validation processes, such as Installation Qualification (IQ), Operational Qualification (OQ), and Performance Qualification (PQ) protocols, are essential to ensure reproducibility and compliance but add time-intensive steps, including accuracy and precision testing on multiple samples.^[69] Space constraints in older facilities further complicate deployment, demanding remodeling for ventilation, structural support, and efficient layouts.^[68] Economic barriers primarily involve high upfront costs for equipment and infrastructure, ranging from $45,000 to $300,000 for core instruments, plus $15,000 to $30,000 annually for maintenance, excluding full system integrations that can exceed $1 million.^[69]^[71] These investments, while promising long-term savings through productivity gains (with payback periods around 4.75 years via staff cost reductions), restrict adoption, especially in resource-limited settings.^[18]

Future Trends and Innovations

The integration of artificial intelligence (AI) and machine learning (ML) into laboratory automation is poised to enable predictive maintenance and adaptive workflows, minimizing downtime and enhancing operational efficiency. AI-driven predictive maintenance utilizes sensor data and ML models to forecast equipment failures before they occur, allowing for proactive interventions that extend instrument lifespan and reduce unexpected disruptions in lab processes.^[72] For instance, AI algorithms can optimize liquid handling tasks, such as adjusting pipetting parameters in real-time based on fluid viscosity to ensure precise transfers and minimize errors in viscous sample processing.^[73] These advancements will transform workflows by enabling autonomous adjustments to experimental protocols, accelerating research timelines while maintaining high accuracy. Recent U.S. Food and Drug Administration (FDA) approvals for AI-enabled medical devices in diagnostics as of 2024–2025 have further supported integration in clinical settings.^[74]^[75] Emerging technologies like microfluidics and blockchain are set to further revolutionize laboratory automation. Microfluidic lab-on-a-chip systems integrate multiple laboratory functions into compact, automated platforms, facilitating rapid, miniaturized analyses for applications in diagnostics and drug discovery, with ongoing developments in automation principles enhancing their scalability and reliability.^[76] Blockchain technology addresses data integrity challenges in multi-site laboratories by providing immutable audit trails and secure, decentralized data sharing, ensuring transparency and compliance across distributed research networks without compromising privacy.^[77] These innovations promise to create more robust, interconnected systems that support collaborative, high-throughput experimentation. Sustainability is becoming a core driver in laboratory automation design, with energy-efficient systems and modular architectures reducing environmental impact. Automated platforms incorporating low-energy components and optimized protocols can decrease waste generation by up to 30 tons annually in clinical settings through precise resource allocation and reduced consumable use.^[78] Modular, upgradable systems promote longevity by allowing targeted upgrades rather than full replacements, minimizing electronic waste and supporting circular economy principles in lab infrastructure.^[79] Overall, the laboratory automation market is projected to reach USD 18.39 billion by 2033, growing at a CAGR of 9.3% from 2024, fueled by the rise of interconnected "smart labs" that leverage IoT and AI for seamless, data-driven operations.^[80]

Low-Cost Laboratory Automation

Accessible Tools and DIY Approaches

Accessible tools and DIY approaches in laboratory automation emphasize user-assembled hardware solutions that leverage affordable microcontrollers and 3D printing to enable automation in resource-constrained environments. These systems often utilize platforms like Arduino or Raspberry Pi to control custom pipettors, allowing precise liquid handling at a fraction of commercial costs. For instance, open-hardware designs for automated pipetting stations can be built for under $500 using off-the-shelf components, such as stepper motors and 3D-printed chassis, facilitating tasks like reagent dispensing in multi-well plates.^[81]^[82] Examples of such DIY hardware include 3D-printed robotic arms for sample manipulation and low-cost centrifuges constructed from repurposed parts. A low-cost, open-source 3D-printed liquid dispensing robot like Sidekick, controlled by a Raspberry Pi, enables automated pipetting and sample transfer with high precision, assembled using 3D-printed parts and basic electronics for applications in small-scale labs.^[83] Similarly, microcentrifuges like the SeparateDuino repurpose computer DVD drive motors and an Arduino microcontroller to achieve speeds up to 10,000 rpm, separating biological samples such as cells or macromolecules at a total cost below $25.^[84] These designs prioritize accessibility, with full schematics and code available on repositories like GitHub for replication.^[85] Key advantages of DIY approaches lie in their high degree of customization and rapid prototyping capabilities, allowing users to tailor devices to specific experimental needs without lengthy procurement processes. Unlike commercial systems that may take months to deploy, DIY builds can be assembled in days, using modular components for iterative improvements, such as adapting pipettor volumes or integrating sensors for feedback control. Recent advancements as of 2025 include 3D-printed modular components for self-driving labs, further democratizing automation through open-source designs.^[81]^[82]^[86] This flexibility reduces dependency on proprietary hardware and lowers barriers for non-specialists, enabling precise automation for protocols like serial dilutions while minimizing material waste through on-demand 3D printing.^[83] Case studies demonstrate the impact of these tools in educational settings and resource-limited regions. In STEM education, an Arduino-based liquid handling robot costing around $150 was implemented in middle school labs, where students programmed it via block-based interfaces to perform dilution experiments, fostering skills in coding and precision biology over multiple sessions.^[81] In developing countries, low-cost centrifuges from repurposed electronics have automated sample preparation for assays like ELISA by pelleting antigens or antibodies, enhancing diagnostic workflows in under-equipped facilities without reliable electricity, as seen in portable designs achieving separations equivalent to benchtop models.^[84] These implementations highlight how DIY automation supports basic immunoassays, improving throughput in educational and global health contexts.^[83]

Open-Source Platforms

Open-source platforms in laboratory automation provide collaborative, freely accessible software frameworks that democratize access to programmable robotic systems, enabling researchers to develop and share protocols without proprietary restrictions. These platforms emphasize Python-based programming for flexibility and ease of integration, fostering community contributions that accelerate innovation in low-cost setups. A prominent example is the Opentrons OT-2, an open-source liquid handling robot equipped with a Python API that allows users to script precise pipetting protocols for tasks such as sample preparation and reagent dispensing. The API supports atomic and complex commands for operations like aspiration, dispensing, and tip management, making it suitable for automating repetitive workflows in biology and chemistry labs. Another key platform is PyLabRobot, a hardware-agnostic Python SDK designed for controlling diverse laboratory devices, including liquid handlers, plate readers, pumps, scales, and heater shakers, thereby enabling multi-device orchestration in automated experiments.^[87] It abstracts hardware-specific details through standardized interfaces, allowing protocols to be ported across robots like the Opentrons OT-2, Hamilton STAR, and Tecan EVO without extensive reprogramming.^[88] These platforms feature community-driven libraries that extend functionality, such as modules for integrating sensors to monitor environmental conditions or detect liquid levels during operations. For instance, PyLabRobot includes drivers for real-time sensor feedback, enhancing protocol reliability in dynamic lab environments.^[89] The Opentrons OT-2, typically costing around $15,000 fully configured, delivers substantial savings compared to proprietary commercial equivalents that often exceed $50,000, making high-precision automation viable for resource-limited settings.^[90]^[91] Adoption of these platforms is widespread in academic research, particularly for high-throughput screening (HTS) applications like drug discovery and phenotypic assays, where the Opentrons OT-2 has been used to parallelize thousands of reactions efficiently.^[92] Researchers contribute and share protocols via GitHub repositories, such as the Opentrons Protocol Library, which hosts hundreds of user-submitted scripts for tasks ranging from PCR setup to cell-based assays, promoting reproducibility and collaborative refinement.^[93] The ecosystem surrounding these platforms supports seamless integration with free tools like Jupyter notebooks for interactive protocol development and data analysis, allowing scientists to visualize results and iterate on automation scripts in a single environment. Additionally, adherence to open standards like OPC UA facilitates interoperability with industrial-grade sensors and devices, ensuring robust data exchange in larger automated lab networks.^[94]^[95]

References

[1]
Advances in Clinical Laboratory Automation | myadlm.org
Dec 1, 2014 · Laboratory automation is designed to maximize efficiency and minimize errors by integrating mechanical, electronic, and informatics tools.
[2]
Laboratory Automation in Clinical Microbiology
### Summary of Laboratory Automation in Clinical Microbiology
[3]
The Impact of Total Automaton on the Clinical Laboratory Workforce
May 9, 2022 · 5. A total laboratory automation (TLA) means that a track system connects all aspects of the laboratory process, including the preanalytic, ...
[4]
The first 110 years of laboratory automation - PubMed
The earliest mention of automation in the chemical literature of the United States was in 1875, announcing a device to wash filtrates unattended. In the years ...
[5]
Clinical Chemistry Laboratory Automation in the 21st Century
In the 1956, Leonard Skeggs developed the first practical and completely automated system for measuring urea, glucose, and calcium, the AutoAnalyzer, an ...Early Automated Analysis · Lc-Ms/ms · Informatics
[6]
A Short History of Laboratory Robotics - Montclair State University
By the 1950's scientists were beginning to envision how laboratory processes could be automated. The invention of the transistor and the widespread availability ...
[7]
Automation in the Life Science Research Laboratory - Frontiers
We classify the current levels of automation in laboratories and highlight the benefits and limitations of its usage in research.
[8]
Laboratory Automation - 2023 - Wiley Analytical Science
Feb 21, 2023 · Laboratory automation today is a complex integration of robotics, computers, liquid handling systems and numerous other technologies. The term ...
[9]
Laboratory automation systems. An introduction to concepts and ...
A laboratory automation system consists of robots, conveyor systems, machine vision, and computer hardware and software.
[10]
Transferring Industrial Automation Technology to the Laboratory
Laboratory automation has its own set of requirements and constraints, but careful analysis allows selective transfer of techniques from automation in other ...
[11]
Automation in the Life Science Research Laboratory - PMC - NIH
Benefits and limitations of research laboratory automation. Automation can assist in improving reproducibility in three ways: a reduction in human-induced ...
[12]
Transforming science labs into automated factories of discovery
Oct 23, 2024 · We define five levels of laboratory automation, from laboratory assistance to full automation. ... automated instruments operated by humans ...
[13]
Revolutionizing Laboratory Practices: Pioneering Trends in Total ...
TLA refers to the end-to-end automation of pre-analytical, analytical, and post-analytical processes within a clinical laboratory. The pre-analytical phase ...
[14]
High-Throughput Screening (HTS) - Beckman Coulter
HTS is a method using automation to screen large numbers of biological modulators against targets, producing rich data sets quickly.
[15]
Can I benefit from laboratory automation? A decision aid for the ...
Nov 30, 2023 · A guide to help them decide whether to implement laboratory automation and find a suitable system.Missing: scope | Show results with:scope
[16]
6 Benefits of Leveraging Lab Automation - Hudson
Lab automation can benefit your lab by reducing errors, increasing throughput, enhancing data and saving your money.
[17]
How to reduce laboratory errors with automation - Biosero
Another analysis estimated that automation devices led to a 90-98% decrease in opportunities for error during blood group and antibody testing. That amounts to ...
[18]
Economic Evaluation of Total Laboratory Automation in the Clinical ...
TLA can significantly enhance laboratory performance, has a relatively quick payback period, and can reduce total hospital expenses in the long term.
[19]
Automation to Enable High-throughput Chemical Proteomics - PMC
An automated robotic system represents a major technological opportunity to speed up advances in proteomics, open new frontiers in drug-target discovery.Introduction · Proposed Automation Project... · Fig. 2
[20]
Automated Systems for Improved Safety and Contamination Control ...
May 16, 2024 · Reduced Exposure to Hazardous Materials. One of the major advantages of laboratory automation is its power to greatly reduce human contact ...
[21]
Automated workflows for lab teams: Boost efficiency and productivity
Oct 10, 2025 · Providing smarter way to analyze by eliminating data silos and manual errors: ✓Seamless LIMS integration ✓Built-in ICH M10 compliance ✓5–10X ...
[22]
The First 110 Years of Laboratory Automation - ScienceDirect.com
The earliest mention of automation in the chemical literature of the United States was in 1875, announcing a device to wash filtrates unattended. In the ...
[23]
Laboratory Diagnostics - Siemens Global
1957: AutoAnalyzer ... Frustrated by how long blood tests took to perform in a medical laboratory in the 1950s, biochemist Leonard Tucker Skeggs began tinkering ...
[24]
History of Biochemistry Analyzer - Biomedical Diary
One of the first automated biochemistry analyzers was the Technicon AutoAnalyzer, invented by Leonard Skeggs in 1957. This machine could perform multiple tests ...
[25]
In the Laboratory Automation Zone - BioProcess International
By the early to mid-1980s, microprocessor-driven robots were operating in laboratories. The first robot to appear on the market was made by a company called ...
[26]
Origin and evolution of high throughput screening - PMC
This article reviews the origin and evolution of high throughput screening (HTS) through the experience of an individual pharmaceutical company.
[27]
The History and Evolution of LIMS - Labworks
The need for an efficient lab data management system arose. In 1982, the first LIMS systems were introduced to help automate reporting functions. This ...
[28]
Cultivating DNA Sequencing Technology After the Human Genome ...
Aug 31, 2020 · When the Human Genome Project was completed in 2003, automated Sanger DNA sequencing with fluorescent dye labels was the dominant technology.Missing: post- | Show results with:post-
[29]
Collaborative robots in laboratory automation - Universal Robots
Dec 13, 2023 · Cobots can work alongside human operators (with an appropriate risk assessment) to improve lab productivity and safety.Missing: 2010s | Show results with:2010s
[30]
Lab Automation Market Growth, Drivers, and Opportunities
Global lab automation market valued at $5.97B in 2024, reached $6.36B in 2025, and is projected to grow at a robust 7.2% CAGR, hitting $9.01B by 2030.
[31]
Automated Liquid Handling Systems - Beckman Coulter
These systems range from compact, stand-alone instruments that simplify routine liquid handling tasks to sophisticated, multi-component setups that support ...Biomek FX P Liquid Handler · Biomek i7 · Biomek i5 · Biomek NX P Liquid Handler
[32]
Robotic Arms for Lab Automation - HighRes Biosolutions
Explore Nucleus laboratory robotic arms for seamless automation, featuring various models and powered by Cellario for optimal efficiency.
[33]
https://www.hamiltoncompany.com/automated-sample-storage
[34]
https://highresbio.com/blog/automated-labware-management/modularity-in-motion/
[35]
Modularity to Future-Proof Your Automated Lab
Sep 8, 2023 · Finally, we will look at how HighRes Biosolutions has optimized the modular approach to workflows and laboratory design over time. AD-DIG-240821 ...How Modularity Enables... · The Progression Of Lab... · The Evolution Of Nucleustm
[36]
ISO Standards for automated liquid handling systems - Tecan
ISO/IWA 15 is a specification and method for determining the performance of automated liquid handling systems (ALHS). Standardization maximizes precision and ...
[37]
Selecting a Laboratory Information Management System for ... - NIH
In this article, we review the processes, African experience, lessons learned, and make recommendations for choosing a biorepository LIMS in the African ...
[38]
Ten simple rules for implementing electronic lab notebooks (ELNs)
Jun 20, 2024 · Introduction. An electronic lab notebook (ELN) is a software tool for documenting laboratory experiments, research data, and processes.
[39]
The SiLA 2 Manager for rapid device integration and workflow ...
The SiLA 2 Manager uses the emerging SiLA 2 standard and provides a lean and extendable framework for device discovery, management, and workflow design.
[40]
OPC UA LADS - SLAS
OPC UA LADS can provide plug & play interoperability of lab devices along the workflow and generally uses Ethernet/TCP IP as transport layer.
[41]
PyOpticon: An Open-Source Python Package for Laboratory Control ...
Jun 20, 2025 · In this article, we present PyOpticon, a free and open-source Python package for controlling and acquiring data from benchtop experimental setups.
[42]
The SampleManager LIMS Data Analytics Solution - US
SampleManager LIMS offers data analytics solutions including Autonomous Test Revisor (ATR), AI for predicting results, and Business Intelligence (BI) for lab ...
[43]
Autoverification in a core clinical chemistry laboratory at an ... - NIH
Mar 28, 2014 · Autoverification is a process of using computer-based rules to verify clinical laboratory test results without manual intervention.Missing: handling | Show results with:handling
[44]
Thermo Fisher Connect Platform
The platform connects your scientific environment, provides cloud storage, analysis apps, collaboration tools, asset management, and remote troubleshooting.
[45]
E1578 Standard Guide for Laboratory Informatics - ASTM
Aug 23, 2019 · It explains the evolution of laboratory informatics tools used in today's laboratories such as laboratory information management systems (LIMS), ...
[46]
https://www.clinicallab.com/preanalytical-automation-in-the-clinical-lab-25465
[47]
Preanalytical Automation in the Clinical Lab
Mar 23, 2021 · Lab automation enables traceability of samples and results, thus providing an additional layer of safety to reported results. In light of COVID- ...
[48]
Automated hematology analyzers: Recent trends and applications
The modern day analyzers are providing five to seven parts differential white-cell analysis, based on the different technologies.
[49]
cobas® SARS-COV-2 Test - Roche Diagnostics
The tests are for use on the automated, high throughput cobas® 6800/8800 Systems under Emergency Use Authorization. Features and Benefits of the cobas® SARS ...
[50]
Automation of the Pre-Analytical Phase: A Performance Evaluation ...
This paper is about the automation of the pre-analytical phase in a biochemical laboratory, which performs more than 3.7 million tests per year.
[51]
Automate Complex Processes & Simplify Clinical Lab Operations ...
Automation: Simplify clinical lab management and reduce manual tasks ... Streamlined Workflows: The seamless integration with analyzers, EHR systems ...
[52]
Automated end-to-end blood testing at the point-of-care: Integration ...
The system uses a robotic venipuncture, a sample handling module, and a centrifuge-based blood analyzer for automated end-to-end blood testing.
[53]
[PDF] Geisinger Medical Center Transforms their Laboratory with a State ...
86% fewer discrete processing steps in specimen handling. Achieve consistent and. Turnaround time goals met with one system predictable turnaround times and ...
[54]
Case Studies - Laboratory Diagnostics IT - Siemens Healthineers
Zhongshan People's Hospital has reduced errors by 93%, reduced turnaround times by 77%, and achieved 85% employee satisfaction. Learn how the lab used ...
[55]
CLIA Compliance for Pre-Analytic, Analytic, and Post-Analytic ...
Oct 24, 2018 · CLIA compliance addresses the total testing process (TTP), wherein all phases of the testing cycle are assessed, monitored, and improved in ...
[56]
CLIA Categorizations - FDA
Jul 17, 2023 · Test systems receive an initial CLIA categorization from the FDA after the test system is cleared/approved/licensed/granted following review of ...
[57]
High-Throughput Screening of a 100,000 Compound Library ... - NIH
High-Throughput Screening of a 100,000 Compound Library for Inhibitors of ... We screened a library of 100,000 compounds, which were selected for ...
[58]
Automated high-throughput genome editing platform with an AI ...
Nov 30, 2022 · In this study, we devise an automated high-throughput platform, through which thousands of samples are automatically edited within a week, providing edited ...
[59]
Unlocking the efficiency of genomics laboratories with robotic liquid ...
Oct 20, 2020 · More genomics laboratories are now considering liquid-handling automation to make the sequencing workflow more efficient and cost effective.Missing: calling | Show results with:calling
[60]
Approaches to automated protein crystal harvesting - PMC - NIH
Several automated protein crystal harvesting systems have been developed, including systems utilizing microcapillaries, microtools, microgrippers, acoustic ...
[61]
Accelerating discovery in natural science laboratories with AI and ...
Sep 24, 2025 · (A) Science automation aims to replace labor-intensive tasks with autonomous systems to boost precision, efficiency, and reproducibility, ...
[62]
High Throughput Screening - Beckman Coulter
Our series of Biomek automated workstations provide comprehensive, tailored solutions for your high-throughput screening workflows.
[63]
Engineering biology and automation–Replicability as a design ...
Jul 22, 2024 · The relationship between automation, high throughput and replicability is, unfortunately, more complicated than the basic version in Section 3.1 ...<|separator|>
[64]
Towards Robot Scientists for autonomous scientific discovery - PMC
Greater automation improves the accuracy and reliability of experiments, increases the pace of discovery and, in common with conventional laboratory automation, ...
[65]
What Factors Impact Adoption of Total Laboratory Automation?
Jun 17, 2022 · The challenges of adopting total lab automation. Inconsistencies in how data is stored, as well as incompatibilities between automated modules, ...
[66]
Four common pitfalls to avoid when choosing laboratory automation ...
1. Integration frustration between vendors · 2. Quality gaps · 3. User-interface overload · 4. A tortuous route to compliance · Automation roadblocks? Let Tecan ...
[67]
Challenges and Opportunities in Implementing Total Laboratory ...
Oct 2, 2017 · We invited a group of 5 experts to share their perspectives on laboratory automation and provide real-world advice based on their experiences with TLA.
[68]
Implementing laboratory automation for next-generation sequencing
Jul 13, 2023 · A lab can process 4–384 samples per run, depending on their system and needed output. Another added benefit is that some platforms offer modular ...
[69]
Five Tips to Help Staff Adapt to Change in the Lab
Sep 8, 2023 · Managing change can be a difficult challenge for lab managers who must both envision the improvements and overcome the resistance to change among staff.
[70]
Artificial Intelligence and the Future of Lab Automation - Kalleid
Dec 10, 2024 · AI can address this through predictive maintenance, which can use sensor data and ML models to forecast failures before they occur. Sensors ...
[71]
Trends in Lab Automation Liquid Handling - Dispendix
Mar 18, 2025 · To further increase the accuracy of lab automation liquid handling, AI is increasingly being incorporated into liquid handling systems. Machine ...Contactless Liquid Handling... · Miniaturization And... · Sustainability-Driven...
[72]
HPLC 2025 Preview: The Present and Future of Automation in ...
May 22, 2025 · Five key trends will shape the future laboratory. AI is increasingly used for real-time adjustments of laboratory processes, optimizing ...
[73]
Transformative laboratory medicine enabled by microfluidic ...
Mar 1, 2025 · In this review, we will provide a comprehensive review of microfluidic automation, focusing on the microstructure design and automation principles.
[74]
[PDF] A Blockchain Framework for Managing and Monitoring Data in Multi ...
Feb 11, 2019 · To reduce the administrative burden, time, and effort of ensuring data integrity and privacy in multi-site trials, we propose a novel data.
[75]
Reducing the Environmental Impact of Clinical Laboratories - NIH
The new system also meant that the laboratory operations generated 30 tons less material waste each year, with nearly a million sample tubes saved in a single ...
[76]
Modular Product Architecture for Sustainable Flexible Manufacturing ...
The recyclability, flexibility and functionality of modular products make them more conducive to sustainability. ... Companies can also upgrade and redesign ...
[77]
Laboratory Automation Market Growth, Trends, and Future Forecast ...
Rating 4.6 (38) The global laboratory automation market is anticipated to reach $24.84 billion by 2033, witnessing a CAGR of 9.57% between 2023-2033.Missing: projection | Show results with:projection
[78]
The Future of Smart Labs: From Automation to AI Data Analysis
Oct 16, 2025 · Today, smart laboratory systems integrate IoT sensors, AI algorithms, and cloud connectivity to create interconnected and adaptive ecosystems.
[79]
DIY liquid handling robots for integrated STEM education and life ...
Nov 9, 2022 · Here we investigate the design of a low-cost (~$150) open-source DIY Arduino-controlled liquid handling robot (LHR) featuring plastic laser-cut parts.
[80]
FINDUS: An Open-Source 3D Printable Liquid-Handling Workstation ...
We report the successful 3D printing and assembly of a liquid-handling workstation for less than $400. Using this setup, we achieve reliable and flexible liquid ...Missing: DIY | Show results with:DIY
[81]
Establishment of low-cost laboratory automation processes using ...
In this study, we investigate the feasibility of such a strategy based on a low-cost 4-axis robot and freely available software.
[82]
SeparateDuino: Design and Fabrication of a Low-Cost Arduino ...
Jul 30, 2020 · In this paper, a low-cost and portable microcentrifuge is fabricated using the recycled parts of a computer DVD drive and an Arduino microcontroller for less ...
[83]
https://www.hardware-x.com/article/S2468-0672(22)00064-5/fulltext
[84]
PyLabRobot: An open-source, hardware-agnostic interface for liquid ...
Oct 20, 2023 · PyLabRobot increases the accessibility of laboratory automation by using standardized interfaces that enable a hardware-agnostic programming ...
[85]
PyLabRobot: An Open-Source, Hardware Agnostic Interface for ...
PyLabRobot provides a flexible, open, and collaborative programming environment for laboratory automation. Keywords: Liquid handling robots, open source ...
[86]
PyLabRobot/pylabrobot: interactive & hardware agnostic SDK for lab ...
PyLabRobot is a hardware agnostic, pure Python library for liquid handling robots, plate readers, pumps, scales, heater shakers, and other lab automation ...
[87]
https://www.sciencedirect.com/science/article/pii/S2666998623001709
[88]
Adapting a Low-Cost and Open-Source Commercial Pipetting Robot ...
At a total materials cost of less than $6000, including the commercial liquid handler and all modifications, this system is also far less expensive than other ...
[89]
https://github.com/PyLabRobot/pylabrobot
[90]
Opentrons/Protocols: Repository for Public Protocols - GitHub
This is where Opentrons protocols are stored for everyone to use. The master branch populates http://protocols.opentrons.com/, our Protocol Library.
[91]
Welcome to PyLabRobot's documentation! — PyLabRobot ...
PyLabRobot is a hardware- and operating system-agnostic, pure Python Software Development Kit (SDK) for Automated & Autonomous Laboratories.
[92]
OPC UA LADS - GitHub
We want to help you get started and enable you to enter the strong foundations of the OPC UA ecosystem for your laboratory/analytical devices.Missing: Jupyter | Show results with:Jupyter